##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233040_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81607113 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.197566993945735 31.0 31.0 34.0 26.0 34.0 2 30.879531003627097 33.0 31.0 34.0 27.0 34.0 3 30.3522617691426 33.0 31.0 34.0 25.0 34.0 4 33.75129781885557 37.0 35.0 37.0 28.0 37.0 5 33.88787561937156 37.0 35.0 37.0 28.0 37.0 6 34.05814888954594 37.0 35.0 37.0 30.0 37.0 7 34.234015912313914 37.0 35.0 37.0 31.0 37.0 8 34.23024896126395 37.0 35.0 37.0 31.0 37.0 9 35.75415377088515 39.0 37.0 39.0 30.0 39.0 10-11 35.56426216180445 39.0 35.0 39.0 30.0 39.0 12-13 35.36355318071355 39.0 35.0 39.0 30.0 39.0 14-15 36.52723931674926 40.0 37.0 41.0 30.0 41.0 16-17 36.35165138730002 40.0 36.5 41.0 29.0 41.0 18-19 35.89498250599797 40.0 35.5 41.0 27.5 41.0 20-21 35.43458689685542 40.0 35.0 41.0 26.0 41.0 22-23 35.16285137424234 40.0 35.0 41.0 24.0 41.0 24-25 34.80555216430705 40.0 35.0 41.0 19.0 41.0 26-27 34.25054487591051 40.0 35.0 41.0 9.5 41.0 28-29 33.73991670922117 40.0 34.0 41.0 2.0 41.0 30-31 33.309743986409615 40.0 34.0 41.0 2.0 41.0 32-33 32.97167646403568 39.5 33.0 41.0 2.0 41.0 34-35 32.7423943180051 39.0 33.0 41.0 2.0 41.0 36-37 32.570795863100805 39.0 33.0 41.0 2.0 41.0 38-39 32.496934440016275 39.0 33.0 41.0 2.0 41.0 40-41 32.408459364320365 39.0 33.0 41.0 2.0 41.0 42-43 32.27225382302153 39.0 32.0 41.0 2.0 41.0 44-45 32.116879646263186 39.0 32.0 41.0 2.0 41.0 46-47 31.967058128866782 39.0 32.0 40.5 2.0 41.0 48-49 31.804195425955086 38.5 31.5 40.0 2.0 41.0 50-51 31.586612756414993 38.0 31.0 40.0 2.0 41.0 52-53 31.314995464917377 38.0 30.5 40.0 2.0 41.0 54-55 31.14301000943386 38.0 30.0 40.0 2.0 41.0 56-57 31.00602598820032 38.0 30.0 40.0 2.0 41.0 58-59 30.840906741793454 38.0 30.0 40.0 2.0 41.0 60-61 30.614213328438662 38.0 29.0 40.0 2.0 41.0 62-63 30.337389284926672 37.0 29.0 40.0 2.0 41.0 64-65 29.99550859616857 37.0 28.0 40.0 2.0 41.0 66-67 29.69272476897939 36.0 28.0 39.0 2.0 40.5 68-69 29.349031107129107 36.0 28.0 39.0 2.0 40.0 70-71 28.900560696222644 35.5 26.5 39.0 2.0 40.0 72-73 28.393270112373663 35.0 26.0 38.0 2.0 40.0 74-75 27.99923140890917 34.5 26.0 37.5 2.0 39.5 76-77 27.17671395874524 34.0 25.0 36.0 2.0 39.0 78-79 27.28145884783352 34.0 25.0 36.0 2.0 39.0 80-81 27.04028424581078 34.0 24.5 36.0 2.0 38.5 82-83 26.686208137273525 34.0 23.5 36.0 2.0 37.5 84-85 26.251691130159205 33.5 21.5 35.0 2.0 37.0 86-87 25.795179165325944 33.0 20.0 35.0 2.0 36.5 88-89 25.376234495637654 33.0 18.0 35.0 2.0 36.0 90-91 24.956479816900277 32.0 13.5 34.5 2.0 35.5 92-93 24.48267658972325 32.0 4.5 34.0 2.0 35.0 94-95 24.085268155485416 32.0 2.0 34.0 2.0 35.0 96-97 23.620331814703455 31.0 2.0 34.0 2.0 35.0 98-99 22.93388701301074 31.0 2.0 34.0 2.0 35.0 100-101 20.22034394844969 27.0 2.0 32.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5964411845662951 1101 2 1.2315793794514072 1101 3 1.7805290317935842 1101 4 1.7233294230457261 1101 5 1.424234336918417 1101 6 1.2295454855648345 1101 7 1.098703003783612 1101 8 1.0857580874245372 1101 9 1.2570502377256076 1101 10-11 1.3044017441844389 1101 12-13 1.4184433616553918 1101 14-15 1.5821712655626783 1101 16-17 1.546863959101735 1101 18-19 1.6103089240744026 1101 20-21 1.662960568929698 1101 22-23 1.5125034527205514 1101 24-25 1.540271082398263 1101 26-27 1.5693423194850098 1101 28-29 1.5786797431876565 1101 30-31 1.5920462510583206 1101 32-33 1.5776982239597714 1101 34-35 1.614002997993147 1101 36-37 1.6464343305349658 1101 38-39 1.6341147944650842 1101 40-41 1.6303356147580956 1101 42-43 1.6136452389457503 1101 44-45 1.687240169238251 1101 46-47 1.6325657606762363 1101 48-49 1.5677387531456723 1101 50-51 1.6130175906346658 1101 52-53 1.6901126203374446 1101 54-55 1.7555718519587877 1101 56-57 1.715167214057221 1101 58-59 1.7088596857465888 1101 60-61 1.6621329274479457 1101 62-63 1.6761608403287163 1101 64-65 1.7102740107951035 1101 66-67 1.682762151418821 1101 68-69 1.6458058321099003 1101 70-71 1.6577911900195552 1101 72-73 1.7460495876465387 1101 74-75 1.6238162415800232 1101 76-77 1.6006233337883522 1101 78-79 1.6972584160460507 1101 80-81 1.648698900944762 1101 82-83 1.621399934048977 1101 84-85 1.5851929216327534 1101 86-87 1.5756561416159371 1101 88-89 1.67200065445968 1101 90-91 1.6717839871838507 1101 92-93 1.6091157474117388 1101 94-95 1.5530073196073175 1101 96-97 1.641062331347154 1101 98-99 1.6148355289727832 1101 100-101 1.7892835662766906 1102 1 1.4305865976661387 1102 2 1.0774650203938911 1102 3 1.636239149765622 1102 4 1.582526118881212 1102 5 1.2358100848990148 1102 6 1.0444892375853314 1102 7 0.9429955039211819 1102 8 0.9007207362460363 1102 9 1.0341638966576596 1102 10-11 1.1081690698206543 1102 12-13 1.1936664089639493 1102 14-15 1.2721320668024987 1102 16-17 1.2554884668935742 1102 18-19 1.2785303992897994 1102 20-21 1.2868404113618865 1102 22-23 1.1990957209225073 1102 24-25 1.2487098586110932 1102 26-27 1.3137665567640227 1102 28-29 1.3476409865693952 1102 30-31 1.3490062500326516 1102 32-33 1.3396397072523882 1102 34-35 1.3443122763303847 1102 36-37 1.354382539975397 1102 38-39 1.367334981223813 1102 40-41 1.3386565105205008 1102 42-43 1.3657944536327875 1102 44-45 1.4176814132757656 1102 46-47 1.402913099123687 1102 48-49 1.4088186201105977 1102 50-51 1.3996377223221792 1102 52-53 1.4680872043860802 1102 54-55 1.5050010205517275 1102 56-57 1.4935196865343734 1102 58-59 1.5005962029804039 1102 60-61 1.4676227865958928 1102 62-63 1.4743823883004303 1102 64-65 1.4753994278594007 1102 66-67 1.5031280129651776 1102 68-69 1.4492270649471912 1102 70-71 1.4293886977640042 1102 72-73 1.4997156261281148 1102 74-75 1.3783557714661505 1102 76-77 1.3371810716510986 1102 78-79 1.474581354943215 1102 80-81 1.4469468354815191 1102 82-83 1.4151375111432962 1102 84-85 1.3822273912279854 1102 86-87 1.3966799368434515 1102 88-89 1.5102308050810578 1102 90-91 1.4822662746325257 1102 92-93 1.3916248924496522 1102 94-95 1.397911167280462 1102 96-97 1.48500574189449 1102 98-99 1.4443734915272088 1102 100-101 1.6418742697210575 1103 1 1.2447537300424578 1103 2 0.9122379580624269 1103 3 1.480549996334993 1103 4 1.4547738640253698 1103 5 1.07302461269407 1103 6 0.9226212986556774 1103 7 0.808599716281293 1103 8 0.7779946042065404 1103 9 0.8604777348631885 1103 10-11 0.8810856098094462 1103 12-13 0.9859454564597812 1103 14-15 1.0946990599903614 1103 16-17 1.0654691344983576 1103 18-19 1.0822277463709824 1103 20-21 1.1465419047233851 1103 22-23 1.104172190291159 1103 24-25 1.069651574411509 1103 26-27 1.0705502725171812 1103 28-29 1.054764434567197 1103 30-31 1.0636629963169497 1103 32-33 1.0498459533274982 1103 34-35 1.083576716650164 1103 36-37 1.0651252307294854 1103 38-39 1.075371862894876 1103 40-41 1.0578096976397688 1103 42-43 1.0605546912038264 1103 44-45 1.1248266980004686 1103 46-47 1.108284882550116 1103 48-49 1.1134423939861016 1103 50-51 1.1293189013889027 1103 52-53 1.1922111708413325 1103 54-55 1.1956242366098984 1103 56-57 1.0964334968247051 1103 58-59 1.134250129704867 1103 60-61 1.1370323793875166 1103 62-63 1.1257003486399277 1103 64-65 1.1821750788138914 1103 66-67 1.1646844266415393 1103 68-69 1.1094729846597922 1103 70-71 1.1089095640243833 1103 72-73 1.2006088074770354 1103 74-75 1.0958597718483851 1103 76-77 1.0349489563047207 1103 78-79 1.1695179051619142 1103 80-81 1.0621452743451663 1103 82-83 1.0539286670559882 1103 84-85 1.0186526562949467 1103 86-87 1.0574055587517108 1103 88-89 1.1444041435765797 1103 90-91 1.0288268356519197 1103 92-93 0.99348504953279 1103 94-95 1.0013866004904735 1103 96-97 1.030654808718534 1103 98-99 1.0169611326613897 1103 100-101 1.2114570716398738 1104 1 1.0995292665646978 1104 2 0.7548413238072804 1104 3 1.3007012669523554 1104 4 1.298848186453064 1104 5 0.8922267504529344 1104 6 0.7268283622957341 1104 7 0.6589556710873552 1104 8 0.6189804800391059 1104 9 0.6448069947996729 1104 10-11 0.6588277738439459 1104 12-13 0.7543435330053114 1104 14-15 0.8432474329008954 1104 16-17 0.8238149000276707 1104 18-19 0.8544507753059847 1104 20-21 0.8814886447284707 1104 22-23 0.7973038401153687 1104 24-25 0.8172449518337999 1104 26-27 0.815445794592975 1104 28-29 0.7822979805192602 1104 30-31 0.8255876229475092 1104 32-33 0.8561791990520078 1104 34-35 0.8233933110372433 1104 36-37 0.8047766011116977 1104 38-39 0.8035249585769648 1104 40-41 0.8034155305447541 1104 42-43 0.7980941709104528 1104 44-45 0.8481912609569306 1104 46-47 0.8256504026415072 1104 48-49 0.7960655487861423 1104 50-51 0.8327074711408606 1104 52-53 0.9372055558785704 1104 54-55 0.8869133208429183 1104 56-57 0.8453165714473023 1104 58-59 0.880136423794049 1104 60-61 0.8304375367034105 1104 62-63 0.8221458384327498 1104 64-65 0.8652676001146453 1104 66-67 0.8367739917372816 1104 68-69 0.7983463188916566 1104 70-71 0.8290783846251983 1104 72-73 0.9462951286073604 1104 74-75 0.7703733279689082 1104 76-77 0.7698968464895444 1104 78-79 0.8712119857414713 1104 80-81 0.7937405539982585 1104 82-83 0.7952928946073676 1104 84-85 0.7851867835777426 1104 86-87 0.8068091386537901 1104 88-89 0.8250405592688956 1104 90-91 0.8314905745723244 1104 92-93 0.7299081681925905 1104 94-95 0.7675199530356558 1104 96-97 0.7652937141366358 1104 98-99 0.641570419868259 1104 100-101 0.8872206179848163 1105 1 0.7926331913659652 1105 2 0.51477907823649 1105 3 1.0732641333302446 1105 4 1.1071551251129534 1105 5 0.6489750099481952 1105 6 0.4436206526780637 1105 7 0.3957095356838849 1105 8 0.35313567394953793 1105 9 0.3357670678592868 1105 10-11 0.32157511462445854 1105 12-13 0.39769616765318716 1105 14-15 0.4434622327854214 1105 16-17 0.4353076552724531 1105 18-19 0.49624977942322346 1105 20-21 0.5356648230766154 1105 22-23 0.47397771994377536 1105 24-25 0.43655984206542087 1105 26-27 0.4059026004234738 1105 28-29 0.3999821320819805 1105 30-31 0.3873946998275528 1105 32-33 0.4269630403186824 1105 34-35 0.40778297753924875 1105 36-37 0.3973000040589483 1105 38-39 0.3986833832789003 1105 40-41 0.40267140906306764 1105 42-43 0.3983407630063027 1105 44-45 0.4355530913761996 1105 46-47 0.4522505140327624 1105 48-49 0.4073818080257574 1105 50-51 0.4347754866156528 1105 52-53 0.46214624494932366 1105 54-55 0.45072800508013344 1105 56-57 0.364878107027387 1105 58-59 0.42166261786783465 1105 60-61 0.4405836659855247 1105 62-63 0.37655735845217464 1105 64-65 0.4772074019281831 1105 66-67 0.5038033578586152 1105 68-69 0.45808904320698574 1105 70-71 0.43081169089719396 1105 72-73 0.5225833700595928 1105 74-75 0.4068414952374724 1105 76-77 0.3810888726451971 1105 78-79 0.3797441050907615 1105 80-81 0.36837156545432137 1105 82-83 0.35672352852382616 1105 84-85 0.3463692695811531 1105 86-87 0.3578707471872278 1105 88-89 0.36719541863663707 1105 90-91 0.3445900964036781 1105 92-93 0.3014346095534144 1105 94-95 0.31130423958644826 1105 96-97 0.2760100163104191 1105 98-99 0.15572538315262108 1105 100-101 0.4473695990550013 1106 1 0.5101534057565651 1106 2 0.24530554143479932 1106 3 0.8026821594481355 1106 4 0.8396042286144123 1106 5 0.3908936124913893 1106 6 0.1554136377337869 1106 7 0.10772386195742456 1106 8 0.08937418981169998 1106 9 -0.045435066787483436 1106 10-11 -0.017724807542506937 1106 12-13 0.013099037891755927 1106 14-15 -0.06987848786340578 1106 16-17 -0.144039913775714 1106 18-19 -0.07780948337693161 1106 20-21 0.005330264155752218 1106 22-23 -0.02125307775970242 1106 24-25 -0.08540181563861893 1106 26-27 -0.10752803039773795 1106 28-29 -0.11770409624494249 1106 30-31 -0.08425892836632443 1106 32-33 -0.08273396769426711 1106 34-35 -0.1416683459642556 1106 36-37 -0.12192189040258938 1106 38-39 -0.12348920751436054 1106 40-41 -0.1298588246997383 1106 42-43 -0.1383392688053391 1106 44-45 -0.08667805415009155 1106 46-47 -0.11231387928250314 1106 48-49 -0.2646083147247644 1106 50-51 -0.282248390425206 1106 52-53 -0.24626153315808352 1106 54-55 -0.35100596866041656 1106 56-57 -0.3766647333916957 1106 58-59 -0.2538828632279646 1106 60-61 -0.2221118929461774 1106 62-63 -0.2545447513537624 1106 64-65 -0.20796401773758078 1106 66-67 -0.1767965813692527 1106 68-69 -0.26680203868147956 1106 70-71 -0.24676102829576152 1106 72-73 -0.229367751570571 1106 74-75 -0.3585446684760356 1106 76-77 -0.38813345832934587 1106 78-79 -0.1882081978586605 1106 80-81 -0.2206189347594254 1106 82-83 -0.2596489107011912 1106 84-85 -0.2934280937158533 1106 86-87 -0.1936646137577469 1106 88-89 -0.19399445395339754 1106 90-91 -0.2004867605988565 1106 92-93 -0.21673953755093223 1106 94-95 -0.2473563971272199 1106 96-97 -0.291584171874149 1106 98-99 -0.3898749285276395 1106 100-101 -0.08855157543009184 1107 1 -0.6234217051710402 1107 2 -0.6850010219558378 1107 3 -5.043779489626527 1107 4 -1.8248307222974418 1107 5 -1.2955313912706288 1107 6 -1.03098210039974 1107 7 -0.9165025832158875 1107 8 -0.8227221486824661 1107 9 -0.8810435733565285 1107 10-11 -0.928318533980466 1107 12-13 -0.9026256245499837 1107 14-15 -1.0732964275354746 1107 16-17 -1.0870826720453834 1107 18-19 -0.9720603772144827 1107 20-21 -0.945716884749153 1107 22-23 -1.2028239202558453 1107 24-25 -1.2199358336690196 1107 26-27 -1.269624164230251 1107 28-29 -1.2167149031714857 1107 30-31 -1.2203091481848105 1107 32-33 -1.2624065525433323 1107 34-35 -1.3108072117669138 1107 36-37 -1.263486556466944 1107 38-39 -1.288219420933963 1107 40-41 -1.2926553769714175 1107 42-43 -1.2810011487620834 1107 44-45 -1.1636565955234808 1107 46-47 -1.1905864172274647 1107 48-49 -1.29087835896134 1107 50-51 -1.2958505414093473 1107 52-53 -1.216829351605199 1107 54-55 -1.2973949668010611 1107 56-57 -1.247683725993621 1107 58-59 -1.245311867285011 1107 60-61 -1.2065951202805927 1107 62-63 -1.2206876225927097 1107 64-65 -1.3370738087315566 1107 66-67 -1.2113226907590295 1107 68-69 -1.2080336529953009 1107 70-71 -1.2001264114471013 1107 72-73 -1.117687489239806 1107 74-75 -1.30466676507357 1107 76-77 -1.309863161775592 1107 78-79 -1.1360283653173795 1107 80-81 -1.1848933854505042 1107 82-83 -1.2774055410962752 1107 84-85 -1.2262817129743766 1107 86-87 -1.2098365113588976 1107 88-89 -1.29707406318607 1107 90-91 -1.3612598645766703 1107 92-93 -1.3597414643366328 1107 94-95 -1.3283803016089557 1107 96-97 -1.3941134933643973 1107 98-99 -1.6175783182190564 1107 100-101 -1.2716855178839772 1201 1 1.524707794033496 1201 2 1.1861751863018135 1201 3 1.7522959940047471 1201 4 1.6853923720935455 1201 5 1.3613228813760259 1201 6 1.182189839149224 1201 7 1.0587839056324313 1201 8 1.0371478470075601 1201 9 1.176722578291134 1201 10-11 1.204494738280708 1201 12-13 1.3168659747371692 1201 14-15 1.434909005094113 1201 16-17 1.4105816228530017 1201 18-19 1.443520726138999 1201 20-21 1.4678827772066754 1201 22-23 1.3778500647894205 1201 24-25 1.372772812227133 1201 26-27 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66-67 -5.029270268737719 2307 68-69 -5.043878591992037 2307 70-71 -4.94904095464241 2307 72-73 -4.804052989050746 2307 74-75 -4.924145313944333 2307 76-77 -5.152382098231566 2307 78-79 -5.120893869452967 2307 80-81 -4.878459290861613 2307 82-83 -4.927532009600849 2307 84-85 -4.9193374296410965 2307 86-87 -4.831089756606698 2307 88-89 -4.9151296375515585 2307 90-91 -4.891988089649427 2307 92-93 -4.998282055961095 2307 94-95 -4.932765455900633 2307 96-97 -4.790659312894128 2307 98-99 -4.7012608046960445 2307 100-101 -3.977518305576588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2015680.0 3 601292.0 4 433523.0 5 468848.0 6 893876.0 7 830375.0 8 721305.0 9 1096483.0 10 1692168.0 11 1632064.0 12 1106232.0 13 736442.0 14 586203.0 15 528112.0 16 494111.0 17 468381.0 18 455589.0 19 450073.0 20 462201.0 21 484458.0 22 524081.0 23 579586.0 24 650011.0 25 739325.0 26 848255.0 27 975880.0 28 1058821.0 29 1231058.0 30 1465818.0 31 1777821.0 32 2168799.0 33 2783139.0 34 3760870.0 35 5473926.0 36 9165202.0 37 1.6839916E7 38 1.4384856E7 39 1022315.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.8908246963906 17.285779756330715 9.011640572478607 39.811754974800074 2 15.678509940741293 16.751090452196834 53.94942189886005 13.620977708201826 3 19.599776312796624 21.38986335136181 37.54961442312893 21.460745912712632 4 20.81665969484792 26.16718839202166 35.57804575196772 17.438106161162693 5 19.59038471537119 29.22977876278453 37.92673900684954 13.25309751499474 6 15.270042038929049 34.16426798497222 37.51666386278066 13.049026113318076 7 11.963862066366014 19.541979563763697 55.13941121373739 13.354747156132895 8 12.352730604400566 22.169387723867157 49.32647727261726 16.15140439911501 9 13.67406086765649 21.981252074505715 47.78094748955262 16.563739568285182 10-11 16.15293203669636 31.19206471622149 38.36778847941845 14.287214767663697 12-13 15.788794047891388 25.55369346297056 43.258909306104236 15.398603183033813 14-15 16.04335825481291 27.976054611808166 41.38932472712275 14.591262406256181 16-17 17.6217660095389 27.68115200938428 39.5961066531051 15.100975327971716 18-19 17.90049661479876 27.911454850755472 38.977237805238865 15.2108107292069 20-21 18.22674513678728 28.205269312737478 37.71623436795271 15.851751182522534 22-23 17.647981861581112 29.511331322307655 36.98103374885961 15.859653067251624 24-25 17.349652393341387 29.56687150441382 36.16378624262159 16.919689859623197 26-27 17.106237144952548 30.24014162992045 35.6202062199283 17.033415005198695 28-29 17.7275776290959 30.543358331495234 34.57155478763816 17.1575092517707 30-31 17.775982617586187 30.786738253267686 33.92306731041148 17.514211818734644 32-33 17.579868657703752 30.897252485383653 33.46653405045881 18.056344806453787 34-35 17.868343321248986 31.677398799979663 32.9972343041861 17.457023574585254 36-37 18.691943506845732 31.677694493255647 32.60294610857557 17.027415891323045 38-39 18.303385427117544 31.169015127810447 33.53080376636877 16.99679567870324 40-41 18.183869907101517 31.3931881537484 33.63326553898407 16.78967640016601 42-43 17.88291053290184 31.27657475247796 33.70877408877542 17.131740625844778 44-45 18.038559479106475 30.26946860999985 33.90139452581188 17.790577385081797 46-47 17.610193804747404 31.37268705142167 33.70860340151569 17.308515742315233 48-49 17.397362348336166 31.429358572078087 33.67577454622132 17.49750453336442 50-51 17.300013836958765 31.277093331104844 33.47667347502061 17.946219356915776 52-53 18.264549443402785 30.94790485568909 33.04436718805741 17.743178512850715 54-55 18.258226125404075 31.52000773332997 32.34644058457548 17.875325556690473 56-57 18.645554820515066 31.731730169907767 32.283869926644634 17.33884508293253 58-59 18.38479017379556 32.073264360453166 32.27434140003233 17.26760406571895 60-61 18.53783443164381 32.441384893350765 31.05064343768369 17.970137237321737 62-63 18.429658542970458 32.67242007696114 31.768208043443714 17.12971333662469 64-65 18.457102980592506 32.3465916040858 31.66350183891978 17.53280357640191 66-67 18.1713338119181 33.46566301388799 30.783489323212184 17.579513850981726 68-69 17.98858869646264 33.015344083658896 31.35086567294237 17.645201546936097 70-71 17.61659588914816 32.87583216621256 31.481024616370306 18.026547328268972 72-73 17.8782521677522 33.20847383408792 31.00099158684939 17.91228241131049 74-75 18.25325299416875 32.444089495546656 31.330899301710787 17.971758208573814 76-77 17.8375813737129 33.268259922617894 30.85032723866652 18.043831465002686 78-79 17.97378789333614 33.66202295182883 30.81030592273693 17.5538832320981 80-81 18.075180826535142 33.82536106099218 30.71122946353213 17.388228648940544 82-83 18.1444649126357 33.557743211072086 30.874408862413915 17.423383013878304 84-85 17.90956535796079 33.82821083204141 30.588961662208774 17.67326214778903 86-87 17.64695061053895 33.90786860083049 30.82877916324678 17.61640162538378 88-89 17.907987656647116 33.7366278826775 30.662488374453602 17.692896086221776 90-91 17.345644433907307 34.58096356286733 30.396423512979027 17.676968490246335 92-93 17.969897398999894 33.53846656085725 30.685506508494885 17.80612953164796 94-95 17.908354889849186 34.450245737570036 29.891829456457607 17.74956991612317 96-97 17.94499009417881 34.47746047117924 30.167074258073313 17.41047517656864 98-99 18.402163429224156 34.08680837708682 29.77181045833247 17.73921773535655 100-101 18.364898170432507 35.07859973618367 29.028014485420144 17.528487607963676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 27177.0 1 29300.0 2 31625.5 3 39339.5 4 56934.0 5 80911.5 6 107393.5 7 135943.0 8 169080.0 9 203514.5 10 239225.5 11 275557.5 12 311519.0 13 346315.5 14 382259.5 15 418131.5 16 452617.0 17 492072.0 18 539697.5 19 587248.0 20 644147.5 21 724607.5 22 827822.5 23 946587.0 24 1126593.0 25 1392812.5 26 1628327.0 27 1872849.0 28 2115898.5 29 2343862.5 30 2646821.0 31 2969357.0 32 3257786.5 33 3659344.0 34 3919897.0 35 3987686.5 36 3951566.0 37 3815963.5 38 3827253.5 39 3682123.0 40 3450065.0 41 3246239.5 42 2808788.0 43 2383135.5 44 2112614.5 45 1893403.5 46 1717241.5 47 1549593.0 48 1414965.0 49 1266596.5 50 1063239.0 51 859237.0 52 708187.0 53 583743.0 54 466514.5 55 377062.5 56 319488.5 57 273668.0 58 225584.5 59 178526.5 60 130075.5 61 88798.0 62 65747.0 63 50707.0 64 36686.5 65 24499.0 66 16037.5 67 11192.0 68 7553.0 69 5018.5 70 3699.5 71 3012.0 72 2590.0 73 2237.5 74 1825.0 75 1315.5 76 1042.0 77 905.0 78 726.0 79 533.0 80 389.0 81 279.0 82 207.0 83 157.5 84 121.0 85 99.0 86 70.5 87 42.5 88 29.0 89 17.5 90 9.5 91 7.5 92 4.5 93 3.5 94 3.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18469468464103123 2 4.558426175424194E-4 3 3.6638963076662204E-4 4 0.0 5 2.4507667609807494E-6 6 1.642013729857102E-4 7 1.4704600565884496E-5 8 3.5536118034220867E-4 9 3.970242152788814E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.0634584512259364E-6 26-27 7.71991529708936E-5 28-29 0.0013638517024857871 30-31 4.2888418317163115E-5 32-33 2.3894975919562308E-4 34-35 1.9973749101993106E-4 36-37 5.471336793889523E-4 38-39 1.5684907270276796E-4 40-41 0.00484026435293698 42-43 0.007842453635138398 44-45 0.008414094982137157 46-47 0.005791161856197511 48-49 0.005685166193785093 50-51 0.003919388742498463 52-53 0.00922836223847301 54-55 0.008672650875420627 56-57 0.02237427514437375 58-59 0.018079306395754988 60-61 0.011934621434286004 62-63 0.01793409846516688 64-65 0.016115629528519162 66-67 0.025402197477565468 68-69 0.04419222623400487 70-71 0.044996077731606555 72-73 0.052570172406417565 74-75 0.044953802004979636 76-77 0.040546097985355765 78-79 0.042043516476315 80-81 0.036806840599789385 82-83 0.049556954673791724 84-85 0.062086499739305814 86-87 0.05072106888525759 88-89 0.036472923628605755 90-91 0.03809288045761403 92-93 0.04169857105470696 94-95 0.02572386061494419 96-97 0.01861357354964879 98-99 0.015866876702279615 100-101 0.0036645089993564656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8.1607113E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.55261474696271 #Duplication Level Percentage of deduplicated Percentage of total 1 79.67809482050708 21.156617555408896 2 8.592936943706938 4.5632988842238715 3 3.3371325761085355 2.6582878695884746 4 1.7939703298801706 1.9053841214715914 5 1.1229799983974798 1.490902763299654 6 0.8131353448721371 1.2954521729717112 7 0.5832416009749319 1.0840612674562384 8 0.4491592906614382 0.9541082883961773 9 0.35318856358905415 0.8440271875811968 >10 2.607721995621319 13.840778651342717 >50 0.3413072434019588 6.350677951481567 >100 0.27407939793789116 14.562349080709977 >500 0.029944555591411664 5.5071407600742495 >1k 0.019712418037899842 10.14043395722157 >5k 0.0018744091480238578 3.4322753652925893 >10k+ 0.0015205115636189742 10.21420412347914 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC 230069 0.28192272896604 TruSeq Adapter, Index 10 (100% over 50bp) GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT 218558 0.2678173408732153 No Hit GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT 206964 0.2536102459598099 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG 195668 0.2397683152937906 TruSeq Adapter, Index 10 (100% over 49bp) GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT 194860 0.23877820552235443 No Hit ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 140700 0.17241144163499572 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 102629 0.12575987095634666 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 101672 0.12458717906121738 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91663 0.11232231680588921 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 86347 0.10580817875520238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.2876759039374423 0.0 0.0 2.2056900848826745E-5 0.0 2 0.28833393481276565 1.2253833804903747E-6 0.0 2.695843437078824E-5 0.0 3 0.2884025562820731 2.4507667609807494E-6 0.0 2.940920113176899E-5 0.0 4 0.2955269352562441 2.4507667609807494E-6 0.0 3.676150141471124E-5 0.0 5 0.2965109181107779 3.676150141471124E-6 0.0 3.921226817569199E-5 0.0 6 0.2972167389379404 3.676150141471124E-6 0.0 5.269148536108611E-5 0.0 7 0.29995302002657537 3.676150141471124E-6 0.0 6.984685268795136E-5 0.0 8 0.34102542017385173 3.676150141471124E-6 0.0 7.22976194489321E-5 0.0 9 0.3414261205392721 3.676150141471124E-6 0.0 9.312913691726848E-5 0.0 10-11 0.349687042598848 3.676150141471124E-6 0.0 1.0477027903192703E-4 0.0 12-13 0.35527662888895484 3.676150141471124E-6 0.0 1.0844642917339815E-4 0.0 14-15 0.358625601667835 4.901533521961499E-6 0.0 1.2743987157099896E-4 0.0 16-17 0.3604158867867314 4.901533521961499E-6 0.0 1.458206222783546E-4 0.0 18-19 0.36208363356757883 6.126916902451873E-6 0.0 1.6787752312718134E-4 0.0 20-21 0.3640013585580463 8.577683663432623E-6 0.0 1.972867242589503E-4 0.0 22-23 0.36607715800459695 8.577683663432623E-6 0.0 2.0525171623213774E-4 0.0 24-25 0.3679507691933668 9.19037535367781E-6 0.0 2.3466091736390674E-4 0.0 26-27 0.3701043804845786 9.803067043922998E-6 0.0 3.094093035738196E-4 0.0 28-29 0.37244425004962495 9.803067043922998E-6 0.0 3.590373304836798E-4 0.0 30-31 0.37510823351881106 9.803067043922998E-6 0.0 3.884465316154488E-4 0.0 32-33 0.3778261338567387 1.0415758734168185E-5 0.0 3.9641152358863615E-4 0.0 34-35 0.3808718242489475 1.2866525495148933E-5 0.0 4.123415075350111E-4 0.0 36-37 0.383994101102437 1.5317292256129683E-5 0.0 4.331730250033474E-4 0.0 38-39 0.3876053059247421 1.6542675636620058E-5 0.0 4.429760920472704E-4 0.0 40-41 0.3917023752574117 1.7155367326865245E-5 0.0 4.515537757107031E-4 0.0 42-43 0.3960555497166037 1.7155367326865245E-5 0.0 4.889279688156595E-4 0.0 44-45 0.4006868862031671 1.7155367326865245E-5 0.0 5.103721779742411E-4 0.0 46-47 0.40605100195126376 1.838075070735562E-5 0.0 5.428448375572359E-4 0.0 48-49 0.4121478969608936 1.9606134087845995E-5 0.0 5.606128965743464E-4 0.0 50-51 0.41925757133449876 1.9606134087845995E-5 0.0 5.814444140426827E-4 1.2253833804903747E-6 52-53 0.4270834822940005 2.0218825778091183E-5 0.0 5.998251647500384E-4 1.2253833804903747E-6 54-55 0.4359577087355118 2.083151746833637E-5 0.0 6.182059154573941E-4 1.2253833804903747E-6 56-57 0.4456210820740589 2.083151746833637E-5 0.0 6.298470575720526E-4 1.2253833804903747E-6 58-59 0.4564283507982938 2.083151746833637E-5 0.0 6.50678575040389E-4 1.2253833804903747E-6 60-61 0.46899343198184207 2.3894975919562304E-5 0.0 6.647704839160282E-4 1.2253833804903747E-6 62-63 0.4837501113413974 2.5733050990297866E-5 0.0 6.917289182868166E-4 1.2253833804903747E-6 64-65 0.5002909489029467 2.695843437078824E-5 0.0 7.150112025161336E-4 1.2253833804903747E-6 66-67 0.5185540628057753 2.695843437078824E-5 0.0 7.297158030820181E-4 1.2253833804903747E-6 68-69 0.5400276811654886 2.8183817751278616E-5 0.0 7.438077119576574E-4 1.838075070735562E-6 70-71 0.5658281282417135 2.8183817751278616E-5 0.0 7.548361623820707E-4 2.4507667609807494E-6 72-73 0.5959143536912034 2.8183817751278616E-5 6.126916902451874E-7 7.65251921116239E-4 2.4507667609807494E-6 74-75 0.631482331693317 2.8183817751278616E-5 1.2253833804903747E-6 7.922103554870272E-4 2.4507667609807494E-6 76-77 0.6744485618551412 2.8183817751278616E-5 1.2253833804903747E-6 8.736983502896371E-4 2.4507667609807494E-6 78-79 0.7256904186771072 2.8796509441523804E-5 1.2253833804903747E-6 9.098471600141032E-4 2.4507667609807494E-6 80-81 0.7872028263026534 3.0634584512259366E-5 1.2253833804903747E-6 9.380309777653818E-4 2.4507667609807494E-6 82-83 0.8604268355872361 3.3085351273240116E-5 1.2253833804903747E-6 9.631513370654346E-4 2.4507667609807494E-6 84-85 0.9518428865385791 3.431073465373049E-5 1.2253833804903747E-6 9.80919396082545E-4 2.4507667609807494E-6 86-87 1.062980257615534 3.431073465373049E-5 1.2253833804903747E-6 9.913351548167131E-4 2.4507667609807494E-6 88-89 1.198876499895297 3.431073465373049E-5 1.2253833804903747E-6 9.950113049581842E-4 2.4507667609807494E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 76290 0.0 72.571495 1 GCGTGTT 102945 0.0 54.333874 2 CGGTGGT 8005 0.0 51.821556 1 GTTTTTT 1454355 0.0 41.618 1 CCCGAAA 19205 0.0 38.34528 1 CGTGTTT 158995 0.0 35.520138 3 TATGCCG 76195 0.0 34.410564 46-47 TGCCGTC 75335 0.0 33.741333 48-49 CCGAAAC 22140 0.0 33.008705 2 CTCGTAT 82085 0.0 32.046597 42-43 CCGTCTT 79795 0.0 31.994797 50-51 CGGCCTT 6280 0.0 31.7402 1 GTGTTTT 389710 0.0 31.233175 1 ATCTCGT 83410 0.0 31.201231 40-41 CGGCAAC 5995 0.0 30.868513 1 CCCAGAT 40575 0.0 30.565943 1 CGGAGAT 8520 0.0 30.486555 1 TCGTCTA 19560 0.0 29.926857 44-45 ACTAGCT 92430 0.0 29.89294 32-33 TCAACGG 18410 0.0 29.688297 16-17 >>END_MODULE