FastQCFastQC Report
Fri 27 May 2016
SRR1233039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80126331
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT2425330.3026882636121202No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1912120.2386381575365032No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1794700.22398379878394784No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1423970.17771561261178925Illumina Single End PCR Primer 1 (100% over 50bp)
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT1295260.16165222890338007No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA1252740.15634560878620538No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC1204250.15029391524241886No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC1039200.12969519345644318No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT995140.12419637684396158No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT938620.11714251586036055No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG895780.11179595880909611Illumina Single End PCR Primer 1 (100% over 50bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA803990.10034029887129113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC152650.060.9490179
CGCGTGT699700.060.3163531
GCGTGTT896200.047.1084062
AAGACGG203700.046.8580368
GTTTTTT12988750.044.1454051
CCCGAAA169250.038.5652771
ACCACCG84750.037.275231
CCACCGA87000.036.366642
CGGCATA133100.033.57473812-13
CGTGTTT1307100.032.688173
CCGAAAC204150.031.6706452
ACCGAGA103250.030.7801824
CAAAACG947050.029.7681839
GTGTTTT3195300.029.609351
CCGAGAT165950.029.427561
GAGCGGC114050.029.2477659
GACGGCA157550.028.89741910-11
CGGCCTT110700.028.8805771
TATGCGT124500.028.61254594-95
CGAGCTA186850.028.43264612-13
Produced by FastQC (version 0.11.5)