##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233039_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80126331
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6837801421358	34.0	31.0	34.0	30.0	34.0
2	31.89156948918577	34.0	31.0	34.0	30.0	34.0
3	31.830494085645828	34.0	31.0	34.0	30.0	34.0
4	35.08867774065432	37.0	35.0	37.0	33.0	37.0
5	35.0916696909534	37.0	35.0	37.0	33.0	37.0
6	35.032852471430395	37.0	35.0	37.0	32.0	37.0
7	35.09572466259562	37.0	35.0	37.0	33.0	37.0
8	35.060034322549974	37.0	35.0	37.0	32.0	37.0
9	36.66284687364507	39.0	37.0	39.0	32.0	39.0
10-11	36.62755270798559	39.0	37.0	39.0	32.0	39.0
12-13	36.60039898120382	39.0	37.0	39.0	32.5	39.0
14-15	37.89890427529996	41.0	38.0	41.0	32.5	41.0
16-17	37.62172546001139	40.5	38.0	41.0	32.0	41.0
18-19	37.11679475252648	40.0	37.0	41.0	31.0	41.0
20-21	36.68096871301894	40.0	36.5	41.0	29.5	41.0
22-23	36.272071324468854	40.0	36.0	41.0	27.5	41.0
24-25	35.91266397184716	40.0	36.0	41.0	26.0	41.0
26-27	35.472585647282415	40.0	36.0	41.0	22.0	41.0
28-29	35.0587760720006	40.0	36.0	41.0	17.0	41.0
30-31	34.70019215031823	40.0	35.5	41.0	10.0	41.0
32-33	34.61705410547252	40.0	36.0	41.0	9.5	41.0
34-35	34.48074757098263	40.0	35.5	41.0	8.0	41.0
36-37	34.32586390983009	40.0	35.0	41.0	7.0	41.0
38-39	34.21853575823907	40.0	35.0	41.0	4.5	41.0
40-41	34.12452120140132	40.0	35.0	41.0	2.0	41.0
42-43	34.14985530786378	40.0	35.0	41.0	2.0	41.0
44-45	34.110152004838454	40.0	35.0	41.0	2.0	41.0
46-47	33.740380406286164	40.0	35.0	41.0	2.0	41.0
48-49	33.77104069572336	40.0	35.0	41.0	2.0	41.0
50-51	33.17230132501636	39.5	33.5	40.5	2.0	41.0
52-53	33.28903757617455	39.0	34.0	40.5	2.0	41.0
54-55	33.15583199485323	39.5	33.5	41.0	2.0	41.0
56-57	33.12968786752509	39.0	34.0	41.0	2.0	41.0
58-59	32.61661121111361	39.0	32.5	41.0	2.0	41.0
60-61	32.94577700057176	39.0	34.0	41.0	2.0	41.0
62-63	32.82986751883098	39.0	34.0	41.0	2.0	41.0
64-65	32.535829613863136	39.0	33.0	41.0	2.0	41.0
66-67	32.2258901446517	38.0	33.0	41.0	2.0	41.0
68-69	31.93273737318635	37.5	32.5	40.0	2.0	41.0
70-71	31.545199511256794	37.0	32.0	40.0	2.0	41.0
72-73	31.169050065452268	36.5	31.5	39.5	2.0	41.0
74-75	30.740000992932025	36.0	31.0	39.0	2.0	41.0
76-77	30.304091341708883	35.5	31.0	39.0	2.0	40.5
78-79	29.790874843626625	35.0	30.0	37.5	2.0	39.5
80-81	29.127968320426405	35.0	29.0	37.0	2.0	39.0
82-83	28.81293601849809	35.0	29.0	36.5	2.0	39.0
84-85	28.50014172619485	35.0	29.0	36.0	2.0	37.5
86-87	28.053349778364367	35.0	28.5	36.0	2.0	37.0
88-89	27.60495840624476	34.0	26.5	35.0	2.0	37.0
90-91	27.203412246593444	34.0	26.0	35.0	2.0	36.0
92-93	26.62319088340635	34.0	25.0	35.0	2.0	36.0
94-95	26.03603655582333	34.0	21.5	35.0	2.0	35.5
96-97	25.41420509445266	33.5	17.5	35.0	2.0	35.0
98-99	24.699306985615003	33.0	2.0	35.0	2.0	35.0
100-101	23.122705924223588	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4849928105322583
1101	2	0.3960030351552781
1101	3	0.49081258749203727
1101	4	0.5102255266919045
1101	5	0.48291078177700086
1101	6	0.4624436296078258
1101	7	0.36664199167495326
1101	8	0.33617959172911327
1101	9	0.4324650551509066
1101	10-11	0.4858068995362643
1101	12-13	0.49929489411298533
1101	14-15	0.6137911293424665
1101	16-17	0.6073003399828565
1101	18-19	0.6413712608641973
1101	20-21	0.6843404677966376
1101	22-23	0.6983935120104334
1101	24-25	0.6345264755668225
1101	26-27	0.597252174861957
1101	28-29	0.5011831792046308
1101	30-31	0.6260689447898571
1101	32-33	0.6504685889674278
1101	34-35	0.5789209788731569
1101	36-37	0.5910197059043512
1101	38-39	0.5633456427279881
1101	40-41	0.6568392915056194
1101	42-43	0.6120318732091974
1101	44-45	0.6123683141859217
1101	46-47	0.867491053552726
1101	48-49	0.705239163240897
1101	50-51	0.48207847231732615
1101	52-53	0.5347979577460436
1101	54-55	0.9515487603254869
1101	56-57	0.8458559489556734
1101	58-59	1.2092189292835727
1101	60-61	0.7348081762497074
1101	62-63	0.6220202738060365
1101	64-65	0.5968792665336835
1101	66-67	0.598841194552783
1101	68-69	0.5801305868096307
1101	70-71	0.6019161505607293
1101	72-73	0.5275664747695359
1101	74-75	0.5832178947154745
1101	76-77	0.6280643046933996
1101	78-79	0.6505839953249968
1101	80-81	0.885010208586678
1101	82-83	0.8062874959833799
1101	84-85	0.6253777887339957
1101	86-87	0.6672125109429636
1101	88-89	0.6525584880109605
1101	90-91	0.5894036080026979
1101	92-93	0.7068687709295993
1101	94-95	0.7522326544474502
1101	96-97	0.7052071371242405
1101	98-99	0.6281486293121823
1101	100-101	0.7221414133799762
1102	1	0.36830092223557287
1102	2	0.3215249368061244
1102	3	0.31238980504659963
1102	4	0.35487874235835193
1102	5	0.3491874468058569
1102	6	0.36469857940893036
1102	7	0.3359718348481735
1102	8	0.31588173811276476
1102	9	0.4135447145043827
1102	10-11	0.4273322306321532
1102	12-13	0.4346650725206658
1102	14-15	0.541700485236781
1102	16-17	0.5575709184975111
1102	18-19	0.5768753146753696
1102	20-21	0.664582362954981
1102	22-23	0.6823236643829276
1102	24-25	0.5947886669830424
1102	26-27	0.4994092787758717
1102	28-29	0.4670651072629539
1102	30-31	0.4798853640318228
1102	32-33	0.5418637750775446
1102	34-35	0.5049291183472704
1102	36-37	0.5538588194146215
1102	38-39	0.5451141962933619
1102	40-41	0.621508448124267
1102	42-43	0.5680958315454987
1102	44-45	0.5642748645928748
1102	46-47	0.8487935846547998
1102	48-49	0.6316697317617823
1102	50-51	0.5303647684491253
1102	52-53	0.558196204599362
1102	54-55	0.9442759004553167
1102	56-57	0.8742670217474497
1102	58-59	1.2539595279676305
1102	60-61	0.715350945220429
1102	62-63	0.5773768514353108
1102	64-65	0.5927054023263025
1102	66-67	0.5851814862394278
1102	68-69	0.5609806906410597
1102	70-71	0.5562199959317873
1102	72-73	0.5368153467626122
1102	74-75	0.58579554512065
1102	76-77	0.6100147520570189
1102	78-79	0.6681107399695314
1102	80-81	0.9358373224502152
1102	82-83	0.8491534021665252
1102	84-85	0.7380794881634678
1102	86-87	0.749203967376296
1102	88-89	0.7711202539059983
1102	90-91	0.7629635961338579
1102	92-93	0.8773081645006826
1102	94-95	0.9508795453439127
1102	96-97	1.0610746833620865
1102	98-99	1.1173483164441826
1102	100-101	1.2319788852754314
1103	1	0.23355510231391818
1103	2	0.21482463570309918
1103	3	0.2559203170798625
1103	4	0.23941444633298659
1103	5	0.23364054313006477
1103	6	0.21774634762670786
1103	7	0.16037597220905297
1103	8	0.1941400038651011
1103	9	0.20879903864556582
1103	10-11	0.20841339161206207
1103	12-13	0.20158693217762647
1103	14-15	0.22954534667188398
1103	16-17	0.24136767403697945
1103	18-19	0.3045889369517596
1103	20-21	0.3844973505708964
1103	22-23	0.4177905240689981
1103	24-25	0.36486870982998454
1103	26-27	0.335482024649707
1103	28-29	0.3374020545471694
1103	30-31	0.4082780575789613
1103	32-33	0.35696380303646436
1103	34-35	0.26682308812176814
1103	36-37	0.3188618140844497
1103	38-39	0.3326282294901617
1103	40-41	0.37268197774882594
1103	42-43	0.3579911291797302
1103	44-45	0.347200605617779
1103	46-47	0.6035444856657364
1103	48-49	0.4510193029256584
1103	50-51	0.4262997789865395
1103	52-53	0.37018007821727394
1103	54-55	0.7521115906708715
1103	56-57	0.6787078243910614
1103	58-59	1.0767712617704817
1103	60-61	0.5770878722537631
1103	62-63	0.3939750363087384
1103	64-65	0.42301764934812525
1103	66-67	0.37213378840405653
1103	68-69	0.4310168327964803
1103	70-71	0.4543386812314161
1103	72-73	0.4364624712734546
1103	74-75	0.4524862911269736
1103	76-77	0.46484583384314604
1103	78-79	0.5583865270388273
1103	80-81	0.7995408423973878
1103	82-83	0.7223654669109365
1103	84-85	0.5959605611890595
1103	86-87	0.6559216015420049
1103	88-89	0.632127196414686
1103	90-91	0.6732876938415906
1103	92-93	0.7558089709948952
1103	94-95	0.8419470864659004
1103	96-97	0.9207115651979976
1103	98-99	0.9247496302875327
1103	100-101	1.0004792453249252
1104	1	0.08146279525159628
1104	2	0.1091794956471368
1104	3	0.14943805975136826
1104	4	0.12112019205757463
1104	5	0.14034005723637222
1104	6	0.12624933445068365
1104	7	0.10104036339206601
1104	8	0.1131994432198482
1104	9	0.1347939579041295
1104	10-11	0.11369659933539111
1104	12-13	0.1036693508009634
1104	14-15	0.13355380189302224
1104	16-17	0.15762105418707506
1104	18-19	0.16915469358568203
1104	20-21	0.19001577136447878
1104	22-23	0.2478439761727813
1104	24-25	0.15599768713276063
1104	26-27	0.10553340421754598
1104	28-29	0.12768652812205517
1104	30-31	0.232656174419283
1104	32-33	0.15920986120181624
1104	34-35	0.14039043017364605
1104	36-37	0.137212195953488
1104	38-39	0.10833389872330912
1104	40-41	0.17374757589249867
1104	42-43	0.1853076711886672
1104	44-45	0.1352973000168376
1104	46-47	0.4120743105954574
1104	48-49	0.2154121890419134
1104	50-51	0.10057099596674135
1104	52-53	0.08058841256777072
1104	54-55	0.4646029699869416
1104	56-57	0.4382562641943437
1104	58-59	0.8397946342460969
1104	60-61	0.2991935573235267
1104	62-63	0.19244896840736203
1104	64-65	0.19907084723987367
1104	66-67	0.19979269041180459
1104	68-69	0.18989751444414082
1104	70-71	0.18429121282817462
1104	72-73	0.1811971143633606
1104	74-75	0.20422591055171324
1104	76-77	0.2371784308994762
1104	78-79	0.27274124571436076
1104	80-81	0.49747926779762963
1104	82-83	0.4424497736336228
1104	84-85	0.2863090090372644
1104	86-87	0.27236225303300543
1104	88-89	0.2776275107623505
1104	90-91	0.29680867296705316
1104	92-93	0.4181152326228421
1104	94-95	0.4494238648035953
1104	96-97	0.5062234870583175
1104	98-99	0.4507800552401662
1104	100-101	0.4960897704873055
1105	1	-0.07774174745249596
1105	2	-0.03962928673439592
1105	3	-0.019613549808170205
1105	4	-0.0011100671254808958
1105	5	-0.009559800346288228
1105	6	-0.02620263641961884
1105	7	-0.01573704123666886
1105	8	0.005818108130313249
1105	9	-0.03051241733118104
1105	10-11	-0.03565516953531045
1105	12-13	-0.02867583727837797
1105	14-15	-0.0775968866804746
1105	16-17	-0.06815542435062838
1105	18-19	-0.023102003554228645
1105	20-21	-1.9283701863770375E-4
1105	22-23	0.0639935959833835
1105	24-25	-0.01507470218616902
1105	26-27	-0.041781474471811464
1105	28-29	-0.01717387428281114
1105	30-31	0.08962357883756766
1105	32-33	0.009967881170759085
1105	34-35	0.004283275521196117
1105	36-37	-0.011501547095711828
1105	38-39	-0.04210361877829172
1105	40-41	0.001511112407904136
1105	42-43	-0.02662693008255701
1105	44-45	-0.04741567627801402
1105	46-47	0.24844100970679506
1105	48-49	0.01733680290342221
1105	50-51	-0.13892971142261246
1105	52-53	-0.09357535246402904
1105	54-55	0.25298292795916666
1105	56-57	0.14254054728778698
1105	58-59	0.5669021650710775
1105	60-61	0.11241416981525987
1105	62-63	-0.044248254310751634
1105	64-65	-0.01716451691815024
1105	66-67	0.011089292590092725
1105	68-69	0.004759026135836564
1105	70-71	0.018793884818176565
1105	72-73	0.008356210954424625
1105	74-75	0.11172588956548424
1105	76-77	0.1267023660943103
1105	78-79	0.170995991331651
1105	80-81	0.3666370624976061
1105	82-83	0.3027225896658514
1105	84-85	0.14501990806457954
1105	86-87	0.19003207364176333
1105	88-89	0.14973098243351757
1105	90-91	0.21870592192536265
1105	92-93	0.28174997150784975
1105	94-95	0.3246446790666191
1105	96-97	0.35410183200180967
1105	98-99	0.35181900096132424
1105	100-101	0.3983474895892698
1106	1	-0.23576901249881033
1106	2	-0.22082596247399522
1106	3	-0.21798294999128132
1106	4	-0.20588390930791434
1106	5	-0.19861460792827046
1106	6	-0.19430930990596096
1106	7	-0.2476185782808571
1106	8	-0.18093586486108393
1106	9	-0.225607328431785
1106	10-11	-0.2956832281081674
1106	12-13	-0.2961694564300572
1106	14-15	-0.3481628188317316
1106	16-17	-0.3435135714549631
1106	18-19	-0.3617612029512429
1106	20-21	-0.29883899999476427
1106	22-23	-0.2779913029729286
1106	24-25	-0.3982385003099864
1106	26-27	-0.4376465855978964
1106	28-29	-0.3313783031838966
1106	30-31	-0.23363086158374102
1106	32-33	-0.2859272854540862
1106	34-35	-0.29361027865122225
1106	36-37	-0.29455672109839526
1106	38-39	-0.40576769323656237
1106	40-41	-0.315737260028925
1106	42-43	-0.3317420396606465
1106	44-45	-0.4094609435372192
1106	46-47	-0.0942379709810055
1106	48-49	-0.2894943336994018
1106	50-51	-0.5825465866021418
1106	52-53	-0.5605454818663489
1106	54-55	-0.24935443106129895
1106	56-57	-0.2797250078233162
1106	58-59	0.05672150924194597
1106	60-61	-0.4589392905291234
1106	62-63	-0.549936027734752
1106	64-65	-0.6032279389673931
1106	66-67	-0.5251720184663995
1106	68-69	-0.5327702074833311
1106	70-71	-0.5036128956151806
1106	72-73	-0.4426980658106636
1106	74-75	-0.4631314407589855
1106	76-77	-0.3910004767178812
1106	78-79	-0.3538503583630046
1106	80-81	-0.09756244431367023
1106	82-83	-0.11852122247923447
1106	84-85	-0.2870875962883943
1106	86-87	-0.29968234148695316
1106	88-89	-0.4066793222569487
1106	90-91	-0.33678466713259425
1106	92-93	-0.29691414169031916
1106	94-95	-0.28023760702390277
1106	96-97	-0.21444981543728403
1106	98-99	-0.21375529859729525
1106	100-101	-0.1392315122270844
1107	1	-0.6301337574495882
1107	2	-0.4882013857644125
1107	3	-0.5579621890650479
1107	4	-0.6443607778527891
1107	5	-0.5276451489916241
1107	6	-0.5346455523648217
1107	7	-0.49052386372346746
1107	8	-0.4096688218872231
1107	9	-0.5643365716463222
1107	10-11	-0.619410356062879
1107	12-13	-0.6351819607825391
1107	14-15	-0.8102117748844009
1107	16-17	-0.8516882070329785
1107	18-19	-0.787412699350206
1107	20-21	-0.7616542829008708
1107	22-23	-0.7594944030346298
1107	24-25	-0.8133723460547202
1107	26-27	-0.8877121211388541
1107	28-29	-0.8559801059624732
1107	30-31	-0.7739791113632606
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2308	60-61	-1.5654197617121817
2308	62-63	-1.610497030867542
2308	64-65	-1.9284968023164453
2308	66-67	-1.769490909302423
2308	68-69	-1.7524305198853085
2308	70-71	-1.7703253151548886
2308	72-73	-1.7458485399051007
2308	74-75	-1.7754855705254222
2308	76-77	-1.863148825745629
2308	78-79	-1.8251662283731243
2308	80-81	-1.8923835144627041
2308	82-83	-1.9790659144050373
2308	84-85	-2.179591554695879
2308	86-87	-2.374387687705113
2308	88-89	-2.707159935876028
2308	90-91	-2.921879868257733
2308	92-93	-3.20139923187622
2308	94-95	-3.452679230908796
2308	96-97	-3.872793814861307
2308	98-99	-4.341517108679589
2308	100-101	-4.678908761877594
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	915356.0
3	213433.0
4	190380.0
5	215748.0
6	305764.0
7	501155.0
8	815776.0
9	1217305.0
10	1498205.0
11	1348474.0
12	903302.0
13	666087.0
14	540415.0
15	472379.0
16	428052.0
17	412727.0
18	413343.0
19	427951.0
20	455806.0
21	483654.0
22	525479.0
23	583403.0
24	657979.0
25	696830.0
26	710616.0
27	788544.0
28	885837.0
29	1007524.0
30	1177035.0
31	1395200.0
32	1714400.0
33	2172101.0
34	2896878.0
35	4144565.0
36	6554049.0
37	1.2955654E7
38	2.0832899E7
39	7984876.0
40	17150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.732202131595	15.588774382867253	8.262207680540696	45.41681580499705
2	14.282785149935137	16.32849918494797	55.56851183680035	13.820203828316538
3	19.581731164306035	19.92782267438583	38.11325270319031	22.377193458117826
4	20.332221992424813	25.303556654096006	35.7734335960854	18.59078775739378
5	20.41409569248584	29.825564525412346	35.7986322647884	13.96170751731341
6	15.665543323123925	33.41387036948664	36.91953688211974	14.001049425269695
7	13.036527320759808	18.441891291923397	54.43552968932324	14.086051697993565
8	13.112830359180885	21.74635199537955	48.06752019712188	17.073297448317682
9	14.529915246069185	21.266528799862343	47.02449555893114	17.179060395137327
10-11	17.219710975830917	31.368582985833765	36.946574174744114	14.465131863591205
12-13	16.809648259959097	25.387247586273244	41.750692775057516	16.052411378710143
14-15	17.573899767771557	27.360944609939203	39.53558764407064	15.529567978218598
16-17	18.77883116085313	27.668753275618652	37.523296303390744	16.029119260137474
18-19	19.126647380961515	27.937789400007134	36.34056307101557	16.59500014801577
20-21	19.28155180257525	28.65691369379741	35.08651513748043	16.975019366146906
22-23	19.218929650200128	29.231414891707107	35.08943875996395	16.460216698128814
24-25	18.855093160144605	29.69895405698543	34.22459581126243	17.221356971607534
26-27	18.681700436573536	30.398312838802305	33.66957185035601	17.250414874268152
28-29	19.25891902771468	30.07280067572003	32.890669062392675	17.777611234172618
30-31	19.36430803071464	30.483898225795937	32.509075043783945	17.64271869970548
32-33	18.90744497839747	29.965113081911465	32.90201418568689	18.225427754004176
34-35	19.223927713375804	30.770516428999283	31.98385339400811	18.0217024636168
36-37	19.048101645188257	30.59174286200013	32.334265604175854	18.02588988863576
38-39	19.25889470825459	29.91585001657004	32.616094079913815	18.209161195261554
40-41	19.543056714765463	30.07454573084039	32.49154795856175	17.890849595832396
42-43	19.34221273144334	30.11487237515926	32.72246306627408	17.82045182712332
44-45	19.620871168451686	29.542727556950055	32.460994737116486	18.375406537481776
46-47	18.91282564032657	30.692414863206224	31.800269530538916	18.594489965928293
48-49	18.59481356729248	30.446453134741013	32.138978429376394	18.81975486859011
50-51	18.599620491225757	30.327054209731706	32.62565131864843	18.447673980394107
52-53	18.912209959021773	30.527344337371364	31.997340923117644	18.56310478048922
54-55	19.098158434156943	30.909379414680494	30.949414690551176	19.04304746061139
56-57	19.632025221429743	30.7360442651955	31.315502005605495	18.316428507769263
58-59	18.865333249516087	31.440374005486365	31.193037335187174	18.501255409810376
60-61	19.333436234263736	31.819625788697337	30.324369445375353	18.52256853166357
62-63	19.012258512635633	32.440262281429675	30.063993727963144	18.483485477971552
64-65	19.124376491335067	31.290717319811908	30.675304872040353	18.909601316812672
66-67	19.28722279983287	31.961198932542047	30.197294213448288	18.5542840541768
68-69	19.66096374409321	31.43630241482267	30.460930564049438	18.441803277034683
70-71	18.89313617083453	31.40110171131755	30.3534364254976	19.352325692350323
72-73	18.993546266346502	31.57021235523418	30.3681842953874	19.068057083031913
74-75	19.438692701646048	31.04491979213438	30.103754698062712	19.412632808156864
76-77	19.02948204479906	31.418162407204726	30.308077991360012	19.244277556636206
78-79	19.073595811054307	32.23115638266794	29.985777353349995	18.709470452927754
80-81	19.18306234861562	32.15110847861344	30.076987751475308	18.588841421295633
82-83	19.48940959725155	31.739338839698338	30.148228443863477	18.623023119186634
84-85	19.21035405685561	31.851483354033842	30.17858002101169	18.759582568098853
86-87	19.17417732304504	32.1639781156049	30.15785420351956	18.503990357830506
88-89	19.417909625366313	31.77937569589006	30.061601611150106	18.741113067593513
90-91	18.76627130005088	32.43967343403072	29.866998997538836	18.927056268379555
92-93	19.811226396272154	31.587170211195016	30.09664993129406	18.50495346123877
94-95	19.671243873302437	32.12351454521872	29.31399509335083	18.891246488128015
96-97	19.257025401372296	32.576800298637544	29.64735904136099	18.518815258629175
98-99	20.045702790440007	31.93127286649114	29.24705565221491	18.775968690853944
100-101	20.283806669862734	32.72182292021334	28.37576736046855	18.618603049455373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52621.0
1	57860.5
2	55646.5
3	51954.0
4	63994.5
5	84149.5
6	106164.0
7	128691.0
8	153763.5
9	178350.0
10	201740.0
11	223724.0
12	245558.5
13	266572.0
14	291216.0
15	317864.5
16	341547.0
17	368751.5
18	403717.0
19	445098.0
20	497310.5
21	568912.0
22	670990.5
23	787709.5
24	916458.0
25	1103848.5
26	1299183.5
27	1534887.5
28	1779146.0
29	2018779.0
30	2289603.5
31	2449094.5
32	2547064.0
33	2733041.0
34	2993382.5
35	3234691.5
36	3452372.0
37	3526479.5
38	3583919.5
39	3536896.0
40	3543728.0
41	3635777.0
42	3199760.0
43	2689338.5
44	2418393.0
45	2225960.5
46	2105551.0
47	1931669.0
48	1789131.0
49	1672912.5
50	1452534.0
51	1219816.0
52	1059194.0
53	937948.0
54	822308.0
55	708649.5
56	616581.5
57	538568.5
58	471787.5
59	391963.0
60	291838.5
61	208893.5
62	160027.0
63	124466.5
64	89570.0
65	59113.5
66	37887.5
67	25842.5
68	18287.5
69	13255.5
70	12094.5
71	14053.5
72	14730.0
73	17829.5
74	17729.0
75	12599.0
76	10566.5
77	8787.5
78	6916.0
79	4880.5
80	3091.5
81	1887.5
82	1302.5
83	869.5
84	540.0
85	353.0
86	253.0
87	177.5
88	135.0
89	103.5
90	67.0
91	42.0
92	34.0
93	25.5
94	20.5
95	14.0
96	8.0
97	6.5
98	8.5
99	8.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13869473194772888
2	0.15255409610606033
3	0.1584610182637715
4	0.11791754198753966
5	0.1147937249242075
6	0.17671843728873596
7	0.19458771923551574
8	0.18900902875485462
9	0.2054405810744036
10-11	0.2000403587679561
12-13	0.20743929982267625
14-15	0.22551575461504658
16-17	0.258675890201437
18-19	0.2736996655943225
20-21	0.26991002495796296
22-23	0.29704155554058753
24-25	0.2935608021288283
26-27	0.2808252882563661
28-29	0.233763979533769
30-31	0.24255135306270295
32-33	0.29587402423305764
34-35	0.3022408451473961
36-37	0.30715371205502967
38-39	0.3047687282723578
40-41	0.3011999888026821
42-43	0.3107998293345043
44-45	0.3157601213513695
46-47	0.308814838907325
48-49	0.047554904267362495
50-51	0.0711825928982072
52-53	0.09014951651785978
54-55	0.10847557714829098
56-57	0.11344585339867864
58-59	0.09879773479207479
60-61	0.11005371005943101
62-63	0.1025362062316319
64-65	0.10138053120140993
66-67	0.10836887065251996
68-69	0.10513709906422647
70-71	0.09491324393725203
72-73	0.09427300496262583
74-75	0.08063142189800254
76-77	0.09021254199197015
78-79	0.07533228995596966
80-81	0.10279330024483462
82-83	0.1264322211383921
84-85	0.12947741236273505
86-87	0.13197971088929555
88-89	0.12451774436046499
90-91	0.10835576634602175
92-93	0.10271405039125028
94-95	0.10804875116520686
96-97	0.1200678962824343
98-99	0.12034183868970615
100-101	0.12456766552807715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8.0126331E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.644114550132233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01846709446235	18.919658021074547
2	8.277136553557424	3.914111296387969
3	3.116680529437831	2.210734543625845
4	1.785521579878965	1.688683070655653
5	1.1020292003226326	1.3028252325009475
6	0.7820323403307776	1.1094277342013341
7	0.5712222910972011	0.9454231699003843
8	0.4744140442460772	0.89736800050766
9	0.35173496910028595	0.7484815710624944
>10	2.742063275910015	13.158170469630512
>50	0.3856675854785666	6.378373930641448
>100	0.3235536267149096	15.42052492835452
>500	0.03860213907624169	6.291773066386338
>1k	0.026363187483161982	12.045397098095846
>5k	0.0026865149076198405	4.4005216634405455
>10k+	0.0018250679954970206	10.568526203533617
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	242533	0.3026882636121202	No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	191212	0.2386381575365032	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	179470	0.22398379878394784	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	142397	0.17771561261178925	Illumina Single End PCR Primer 1 (100% over 50bp)
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	129526	0.16165222890338007	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	125274	0.15634560878620538	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	120425	0.15029391524241886	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	103920	0.12969519345644318	No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT	99514	0.12419637684396158	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93862	0.11714251586036055	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	89578	0.11179595880909611	Illumina Single End PCR Primer 1 (100% over 50bp)
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	80399	0.10034029887129113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.2356066946332536	0.0	0.0	6.240145951522478E-6	0.0
2	0.23729278206935495	1.2480291903044956E-6	0.0	1.2480291903044956E-5	0.0
3	0.23746376206842668	1.2480291903044956E-6	0.0	1.372832109334945E-5	0.0
4	0.28864793522119464	1.2480291903044956E-6	0.0	1.4976350283653947E-5	0.0
5	0.28978988193032323	1.2480291903044956E-6	0.0	1.8720437854567433E-5	0.0
6	0.2904438492260428	1.2480291903044956E-6	0.0	2.246452542548092E-5	0.0
7	0.29350900891743065	1.2480291903044956E-6	0.0	2.8704671377003398E-5	0.0
8	0.3307689203939714	1.2480291903044956E-6	0.0	2.9952700567307894E-5	0.0
9	0.3314166475437394	1.2480291903044956E-6	0.0	3.868890489943936E-5	0.0
10-11	0.3398539488847929	2.496058380608991E-6	0.0	3.868890489943936E-5	0.0
12-13	0.34428632455415936	3.744087570913487E-6	0.0	3.931291949459161E-5	0.0
14-15	0.34775210161563497	5.616131356370231E-6	0.0	4.804912382672308E-5	0.0
16-17	0.3505495590457025	6.240145951522478E-6	0.0	5.8033357349159044E-5	0.0
18-19	0.3531635561847952	6.240145951522478E-6	0.0	6.864160546674725E-5	0.0
20-21	0.357031822660144	6.240145951522478E-6	0.0	8.112189736979221E-5	0.0
22-23	0.3612645136590617	6.240145951522478E-6	0.0	8.611401413101019E-5	0.0
24-25	0.3643671142261587	6.240145951522478E-6	0.0	1.0296240820012088E-4	0.0
26-27	0.3680600326002697	6.240145951522478E-6	0.0	1.7846817421354286E-4	0.0
28-29	0.3719295471048088	7.488175141826974E-6	0.0	2.283893418257227E-4	0.0
30-31	0.37608298825014214	1.4352335688501699E-5	0.0	2.3462948777724517E-4	0.0
32-33	0.38022145304519184	1.4976350283653947E-5	0.0	2.389975899433109E-4	0.0
34-35	0.38499953280027266	1.4976350283653947E-5	0.0	2.4211766291907212E-4	0.0
36-37	0.38977636452616304	1.4976350283653947E-5	0.0	2.5834204239303055E-4	0.0
38-39	0.39466177479161	1.4976350283653947E-5	0.0	2.639581737494008E-4	0.0
40-41	0.4003821165853707	1.6224379473958444E-5	0.0	2.783105094379025E-4	0.0
42-43	0.4064643868443196	1.684839406911069E-5	0.0	2.8829474296033846E-4	0.0
44-45	0.4131507032313759	1.8096423259415185E-5	0.0	2.9578291810216544E-4	0.0
46-47	0.42744438154793335	1.8720437854567433E-5	0.0	3.762808008768054E-4	0.0
48-49	0.4357774724515965	2.184051083032867E-5	0.0	3.850170052089369E-4	0.0
50-51	0.4442085336466985	2.246452542548092E-5	0.0	3.8876109277985037E-4	0.0
52-53	0.4538813838861535	2.246452542548092E-5	0.0	3.9000912197015487E-4	0.0
54-55	0.4650762057231848	2.246452542548092E-5	0.0	3.931291949459161E-4	1.2480291903044956E-6
56-57	0.478159919739742	2.3712554615785416E-5	0.0	3.981213117071341E-4	1.8720437854567434E-6
58-59	0.49248292674227157	2.496058380608991E-5	0.0	4.0124138468289534E-4	2.496058380608991E-6
60-61	0.507231511698695	2.6832627591546653E-5	1.2480291903044956E-6	4.056094868489611E-4	2.496058380608991E-6
62-63	0.5237123611712609	2.8704671377003398E-5	1.2480291903044956E-6	4.15593720371397E-4	2.496058380608991E-6
64-65	0.5410312622451163	2.9952700567307894E-5	1.2480291903044956E-6	4.374342312017257E-4	2.496058380608991E-6
66-67	0.5605585509712157	3.244875894791689E-5	1.2480291903044956E-6	4.517865668902274E-4	2.496058380608991E-6
68-69	0.5820034365482178	3.494481732852587E-5	1.2480291903044956E-6	4.5241058148537964E-4	2.496058380608991E-6
70-71	0.6075724345845812	3.619284651883037E-5	1.2480291903044956E-6	4.6052277122235886E-4	2.496058380608991E-6
72-73	0.6354615188857207	3.681686111398262E-5	1.2480291903044956E-6	4.623948150078156E-4	2.496058380608991E-6
74-75	0.6676650400977426	3.7440875709134866E-5	1.2480291903044956E-6	4.711310193399471E-4	3.120072975761239E-6
76-77	0.706894965651179	3.7440875709134866E-5	1.2480291903044956E-6	5.36652551830933E-4	3.744087570913487E-6
78-79	0.7520424216104441	3.8064890304287114E-5	1.2480291903044956E-6	5.634851794224798E-4	3.744087570913487E-6
80-81	0.8042362503781684	3.931291949459161E-5	1.2480291903044956E-6	5.884457632285697E-4	3.744087570913487E-6
82-83	0.8661035533999428	3.993693408974386E-5	1.2480291903044956E-6	6.077902156782893E-4	3.744087570913487E-6
84-85	0.9414140028450823	3.993693408974386E-5	1.2480291903044956E-6	6.109102886540506E-4	3.744087570913487E-6
86-87	1.0349138537243143	4.1184963280048356E-5	1.2480291903044956E-6	6.115343032492028E-4	3.744087570913487E-6
88-89	1.1481456701168558	4.1184963280048356E-5	1.2480291903044956E-6	6.14654376224964E-4	3.744087570913487E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGC	15265	0.0	60.949017	9
CGCGTGT	69970	0.0	60.316353	1
GCGTGTT	89620	0.0	47.108406	2
AAGACGG	20370	0.0	46.858036	8
GTTTTTT	1298875	0.0	44.145405	1
CCCGAAA	16925	0.0	38.565277	1
ACCACCG	8475	0.0	37.27523	1
CCACCGA	8700	0.0	36.36664	2
CGGCATA	13310	0.0	33.574738	12-13
CGTGTTT	130710	0.0	32.68817	3
CCGAAAC	20415	0.0	31.670645	2
ACCGAGA	10325	0.0	30.780182	4
CAAAACG	94705	0.0	29.768183	9
GTGTTTT	319530	0.0	29.60935	1
CCGAGAT	16595	0.0	29.42756	1
GAGCGGC	11405	0.0	29.247765	9
GACGGCA	15755	0.0	28.897419	10-11
CGGCCTT	11070	0.0	28.880577	1
TATGCGT	12450	0.0	28.612545	94-95
CGAGCTA	18685	0.0	28.432646	12-13
>>END_MODULE