FastQCFastQC Report
Fri 27 May 2016
SRR1233039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80126331
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2485440.31019016707504055TruSeq Adapter, Index 4 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1985170.24775501077167755No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1937270.241776950950119No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1925100.24025809942551843No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1502100.18746646467563827TruSeq Adapter, Index 4 (100% over 49bp)
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT1468970.1833317439681595No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC1162130.14503721629285635No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA1060050.13229733431822804No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC1027150.12819131828212627No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT954810.11916307511946353No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA899270.11223152099651236No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC893420.11150142392018425No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC822960.10270781024529875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT712650.062.7336921
GCGTGTT908550.049.2754332
GTTTTTT12764450.046.0826031
CCCGAAA149100.043.3871231
CGGTGGT105750.041.5470661
CCGAAAC189300.033.7210042
CGTGTTT1351000.033.4612543
GTGTTTT3328700.031.0644871
CGAAACC216050.029.6118243
CGGCCTT98600.029.1594331
CGAGCTA178500.028.43070212-13
CCGAGAT108250.028.2749671
CAAAACG885850.028.2500559
TCGTCTA176750.028.16296644-45
CCCAGAT458200.027.6133381
CGGAGAT97600.027.5560931
CTAACGA168800.027.51019194-95
TATGCGT145000.027.406194-95
ACTCGTC187400.026.4990942-43
CTCGTAT890250.026.4340942-43
Produced by FastQC (version 0.11.5)