##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233039_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80126331
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.565020904301733	34.0	31.0	34.0	28.0	34.0
2	31.860474841909333	34.0	31.0	34.0	29.0	34.0
3	31.988942486334487	34.0	31.0	34.0	29.0	34.0
4	35.28605394399002	37.0	35.0	37.0	32.0	37.0
5	35.110454227587184	37.0	35.0	37.0	32.0	37.0
6	35.0328409021998	37.0	35.0	37.0	32.0	37.0
7	34.932304600344175	37.0	35.0	37.0	32.0	37.0
8	34.884689378826046	37.0	35.0	37.0	32.0	37.0
9	36.51322024216983	39.0	37.0	39.0	32.0	39.0
10-11	36.44743724132333	39.0	37.0	39.0	32.0	39.0
12-13	36.23349277655057	39.0	37.0	39.0	32.0	39.0
14-15	37.747037287904774	40.5	38.0	41.0	32.0	41.0
16-17	37.55017125394148	40.5	38.0	41.0	32.0	41.0
18-19	37.167501217546075	40.0	37.5	41.0	31.0	41.0
20-21	36.74272040485667	40.0	37.0	41.0	30.0	41.0
22-23	36.43159550759912	40.0	37.0	41.0	29.5	41.0
24-25	36.09911356729912	40.0	37.0	41.0	27.0	41.0
26-27	35.630951746686115	40.0	36.0	41.0	23.0	41.0
28-29	35.20040568811269	40.0	36.0	41.0	17.0	41.0
30-31	34.811177139759465	40.0	36.0	41.0	9.0	41.0
32-33	34.49851111365626	40.0	36.0	41.0	4.5	41.0
34-35	34.288119257575886	40.0	36.0	41.0	2.0	41.0
36-37	34.12235998925247	40.0	35.0	41.0	2.0	41.0
38-39	33.99267697032078	40.0	35.0	41.0	2.0	41.0
40-41	33.91730474967087	40.0	35.0	41.0	2.0	41.0
42-43	33.65323527043813	40.0	35.0	41.0	2.0	41.0
44-45	33.49793685174478	40.0	34.5	41.0	2.0	41.0
46-47	33.34613073697334	40.0	34.0	41.0	2.0	41.0
48-49	33.192691912974276	39.0	34.0	41.0	2.0	41.0
50-51	32.99148894637395	39.0	34.0	40.0	2.0	41.0
52-53	32.87427176092713	39.0	33.5	40.0	2.0	41.0
54-55	32.73089873539823	39.0	33.0	40.0	2.0	41.0
56-57	32.52243108448333	38.5	33.0	40.0	2.0	41.0
58-59	32.2556377753026	38.0	33.0	40.0	2.0	41.0
60-61	31.970775455823627	38.0	32.5	40.0	2.0	41.0
62-63	31.628477535056483	38.0	32.0	40.0	2.0	41.0
64-65	31.328885756668427	37.0	31.0	40.0	2.0	41.0
66-67	30.948464762476146	37.0	31.0	39.5	2.0	40.5
68-69	30.592168883909086	36.0	31.0	39.0	2.0	40.0
70-71	30.089828005477997	35.5	30.0	38.5	2.0	40.0
72-73	29.607643266481276	35.0	29.0	38.0	2.0	40.0
74-75	29.109680568051967	35.0	29.0	37.5	2.0	39.0
76-77	28.215661022841545	34.0	28.0	36.0	2.0	39.0
78-79	28.332447388611868	34.0	28.0	36.0	2.0	39.0
80-81	27.956327084288933	34.0	28.0	36.0	2.0	38.0
82-83	27.468668139815364	34.0	26.5	35.5	2.0	37.0
84-85	26.69325453701356	33.0	25.0	35.0	2.0	36.5
86-87	26.591341378154453	33.0	25.5	35.0	2.0	36.0
88-89	26.281142056785306	33.0	25.0	34.5	2.0	36.0
90-91	25.940552800302314	32.0	24.5	34.0	2.0	35.0
92-93	25.417348075004206	32.0	22.5	34.0	2.0	35.0
94-95	24.798133518680643	31.5	19.0	34.0	2.0	35.0
96-97	24.15608235574895	31.0	11.0	34.0	2.0	35.0
98-99	23.517406650006226	31.0	2.0	34.0	2.0	35.0
100-101	20.308377248672475	27.0	2.0	31.5	2.0	33.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.316919153618862
1101	2	0.4039924306677243
1101	3	0.3927644274894888
1101	4	0.36341462455951046
1101	5	0.39911703160050394
1101	6	0.39213462986352
1101	7	0.313618944465766
1101	8	0.24936443112991924
1101	9	0.3545818839112087
1101	10-11	0.49925033961932996
1101	12-13	0.7381574899252925
1101	14-15	0.674452260279125
1101	16-17	0.6138331477117234
1101	18-19	0.5871433241883395
1101	20-21	0.6010290841932289
1101	22-23	0.580904618133097
1101	24-25	0.5946762001477381
1101	26-27	0.5711252613042248
1101	28-29	0.604769565063016
1101	30-31	0.6332157118893562
1101	32-33	0.6520909687498673
1101	34-35	0.6861948902193618
1101	36-37	0.7078448494426155
1101	38-39	0.7424670549320211
1101	40-41	0.6849599717514678
1101	42-43	0.8045934879530279
1101	44-45	0.8543008857964551
1101	46-47	0.8310843876801499
1101	48-49	0.6973292053808109
1101	50-51	0.6934505023366455
1101	52-53	0.6729337029157207
1101	54-55	0.6638518165206762
1101	56-57	0.6740681658321606
1101	58-59	0.6864438062585307
1101	60-61	0.6145530614055374
1101	62-63	0.5613128745488609
1101	64-65	0.5694623773360057
1101	66-67	0.48102821059795886
1101	68-69	0.5043353015374485
1101	70-71	0.5120628139778773
1101	72-73	0.5103049418222412
1101	74-75	0.47289256083274367
1101	76-77	0.29767097755038563
1101	78-79	0.5236004823587415
1101	80-81	0.694102682030902
1101	82-83	0.7791596597059431
1101	84-85	1.1535160009907344
1101	86-87	0.7575588385542105
1101	88-89	0.7158438528190878
1101	90-91	0.6187982609022775
1101	92-93	0.5516530262339181
1101	94-95	0.5623699520409318
1101	96-97	0.6192352940466037
1101	98-99	0.6238803939686228
1101	100-101	0.9719121737709884
1102	1	0.3028299682520341
1102	2	0.34463246668274294
1102	3	0.27385877433138006
1102	4	0.2758678435866315
1102	5	0.2899691046472128
1102	6	0.30791101706849133
1102	7	0.31690790817830106
1102	8	0.3112767904087761
1102	9	0.40060877222784086
1102	10-11	0.41239421285291655
1102	12-13	0.624945536016476
1102	14-15	0.49719623499024834
1102	16-17	0.4554989964540752
1102	18-19	0.4283213484758832
1102	20-21	0.4533477290049106
1102	22-23	0.42751855371981407
1102	24-25	0.39571364012044086
1102	26-27	0.4569492352191631
1102	28-29	0.49545183129256287
1102	30-31	0.49273231137061657
1102	32-33	0.4756612545610679
1102	34-35	0.5775362300472864
1102	36-37	0.568244180542294
1102	38-39	0.5997910181883768
1102	40-41	0.5635798982218745
1102	42-43	0.6850644300535649
1102	44-45	0.6780627347317818
1102	46-47	0.7147950335354665
1102	48-49	0.6142852737036719
1102	50-51	0.6087728429558155
1102	52-53	0.5688785704202317
1102	54-55	0.551381029164574
1102	56-57	0.586967179531463
1102	58-59	0.6117701466104961
1102	60-61	0.5535119772314658
1102	62-63	0.5085404427245521
1102	64-65	0.4676208244257296
1102	66-67	0.4426464084917292
1102	68-69	0.4369948715567382
1102	70-71	0.4220483606864711
1102	72-73	0.44258293778012714
1102	74-75	0.483572853197753
1102	76-77	0.4747112449307629
1102	78-79	0.4830141155439236
1102	80-81	0.6451653307673659
1102	82-83	0.7334696349382632
1102	84-85	1.1179691046160016
1102	86-87	0.7029414613432259
1102	88-89	0.6799543294428467
1102	90-91	0.6030391550009533
1102	92-93	0.49916867887985816
1102	94-95	0.6366062536368737
1102	96-97	0.6909111743715428
1102	98-99	0.7505478031738839
1102	100-101	0.9963250568915427
1103	1	0.1521779658157314
1103	2	0.23248640657723385
1103	3	0.19382616485225412
1103	4	0.19762382350635477
1103	5	0.16121713849084784
1103	6	0.17714590406178132
1103	7	0.16666407417424978
1103	8	0.17503950782755595
1103	9	0.24398355097368096
1103	10-11	0.2618028330987485
1103	12-13	0.4514611586681454
1103	14-15	0.37677808612941277
1103	16-17	0.31597206210340545
1103	18-19	0.29978971408491617
1103	20-21	0.3173336725160141
1103	22-23	0.32960434861708876
1103	24-25	0.2834976574303525
1103	26-27	0.2560883840530792
1103	28-29	0.2886002271882333
1103	30-31	0.3154902342131649
1103	32-33	0.3318619730941563
1103	34-35	0.3735885531225378
1103	36-37	0.3850552692162239
1103	38-39	0.367262475790632
1103	40-41	0.3185938391328591
1103	42-43	0.43059807583229315
1103	44-45	0.4813377439390649
1103	46-47	0.5184342223731235
1103	48-49	0.39047587837773534
1103	50-51	0.3791659966560701
1103	52-53	0.37663689074600626
1103	54-55	0.3156618931684534
1103	56-57	0.3064656835393649
1103	58-59	0.28574440190507744
1103	60-61	0.30751181373982917
1103	62-63	0.32794221254623324
1103	64-65	0.2592726277318498
1103	66-67	0.22138694402809023
1103	68-69	0.20089126720811734
1103	70-71	0.232108984904972
1103	72-73	0.2218461007710708
1103	74-75	0.24829223382330312
1103	76-77	0.21967231682603838
1103	78-79	0.24838838088064108
1103	80-81	0.33796387240031933
1103	82-83	0.4506124708880286
1103	84-85	0.8431610207223414
1103	86-87	0.4222539525085942
1103	88-89	0.3905802455211358
1103	90-91	0.3094985332563063
1103	92-93	0.23966181132779596
1103	94-95	0.36471157367024176
1103	96-97	0.35225876962495306
1103	98-99	0.4211512311808967
1103	100-101	0.817476956736165
1104	1	0.05245512861186441
1104	2	0.13161244938168082
1104	3	0.08175942276866266
1104	4	0.10380229392038132
1104	5	0.08254975272200227
1104	6	0.0627129066820089
1104	7	0.06591623044828054
1104	8	0.06598600667950194
1104	9	0.055319063970884486
1104	10-11	0.09549134055214381
1104	12-13	0.29660626082582553
1104	14-15	0.16513043803661986
1104	16-17	0.11124160508924774
1104	18-19	0.12468480030461393
1104	20-21	0.1059309231700638
1104	22-23	0.10690148025268797
1104	24-25	0.05939298773822088
1104	26-27	0.12101626146870359
1104	28-29	0.1155163716944756
1104	30-31	0.09808918465528649
1104	32-33	0.1322101498322681
1104	34-35	0.19534573569207936
1104	36-37	0.21750146918247282
1104	38-39	0.21152073286733497
1104	40-41	0.12719419770589013
1104	42-43	0.25515093556515467
1104	44-45	0.29684908569932844
1104	46-47	0.3208669399098838
1104	48-49	0.22643235498746606
1104	50-51	0.20120512000457325
1104	52-53	0.18614253051600116
1104	54-55	0.15429893876714118
1104	56-57	0.16289475843690582
1104	58-59	0.17162182297039408
1104	60-61	0.14302036163122267
1104	62-63	0.1397243598844966
1104	64-65	0.10827967281582218
1104	66-67	0.1111233378532468
1104	68-69	0.05261537417982254
1104	70-71	0.030676041135485832
1104	72-73	0.07051933139513622
1104	74-75	0.12451556125786922
1104	76-77	0.07568930645848226
1104	78-79	0.09990952481500415
1104	80-81	0.1654915511950179
1104	82-83	0.30239124347339796
1104	84-85	0.6902882447253411
1104	86-87	0.29171226870734657
1104	88-89	0.23476105270129466
1104	90-91	0.20561710286656876
1104	92-93	0.17299734885604323
1104	94-95	0.25041754218878864
1104	96-97	0.2715641884133788
1104	98-99	0.2807214842168335
1104	100-101	0.5553072998994111
1105	1	-0.10242671188881403
1105	2	-0.027232769437215865
1105	3	-0.07541692774374198
1105	4	-0.024424364846773017
1105	5	-0.07561415518859604
1105	6	-0.06548479798819073
1105	7	0.002330023379521151
1105	8	0.020361326202149144
1105	9	-0.022463297898056567
1105	10-11	-0.041746535683245156
1105	12-13	0.11479692202127723
1105	14-15	-0.022167403112000272
1105	16-17	-0.10912065217743105
1105	18-19	-0.13054262370629743
1105	20-21	-0.13481687659205477
1105	22-23	-0.14949977143285764
1105	24-25	-0.17030780059003092
1105	26-27	-0.1537527196502353
1105	28-29	-0.17931239082461303
1105	30-31	-0.1333354414116812
1105	32-33	-0.11741702870633475
1105	34-35	-0.0753224028317021
1105	36-37	-0.055420773535324486
1105	38-39	-0.10556724982945553
1105	40-41	-0.1447172234229015
1105	42-43	-0.012871687333415593
1105	44-45	0.07430373494344877
1105	46-47	0.08866187378822588
1105	48-49	-0.006870304136960215
1105	50-51	-0.06144209579825599
1105	52-53	-0.13219069545474582
1105	54-55	-0.17556307724930775
1105	56-57	-0.11776926102661633
1105	58-59	-0.10086569029292036
1105	60-61	-0.1373508301124886
1105	62-63	-0.1638467320302901
1105	64-65	-0.20214542459804008
1105	66-67	-0.1947690605721526
1105	68-69	-0.2519527322138231
1105	70-71	-0.24380070977621315
1105	72-73	-0.26907582246992234
1105	74-75	-0.16394516385966185
1105	76-77	-0.1785805635625799
1105	78-79	-0.19617782042466914
1105	80-81	-0.08669205944864089
1105	82-83	0.02554430728093493
1105	84-85	0.4840771006747602
1105	86-87	0.013109289452064132
1105	88-89	-0.034714998060174196
1105	90-91	-0.12359878167803728
1105	92-93	-0.24129031383059285
1105	94-95	-0.11039335848776588
1105	96-97	-0.06704758874462158
1105	98-99	-0.028206640549228723
1105	100-101	0.1584383066467403
1106	1	-0.2533094687698174
1106	2	-0.1478894463830862
1106	3	-0.20339140642838416
1106	4	-0.1273293413087302
1106	5	-0.21515936952970094
1106	6	-0.2091705194578779
1106	7	-0.12510965571092925
1106	8	-0.13454290846377148
1106	9	-0.22699560830347565
1106	10-11	-0.2925744803541832
1106	12-13	-0.14512271044380753
1106	14-15	-0.36373500014306615
1106	16-17	-0.42826874813426485
1106	18-19	-0.4366462519020402
1106	20-21	-0.4394798140939571
1106	22-23	-0.44840270242308833
1106	24-25	-0.5121632385218575
1106	26-27	-0.48296054481507866
1106	28-29	-0.4746185934343785
1106	30-31	-0.507014728594271
1106	32-33	-0.4497508913847028
1106	34-35	-0.420940835846352
1106	36-37	-0.47387458998947096
1106	38-39	-0.37571913398633683
1106	40-41	-0.488147042948583
1106	42-43	-0.35846987812054465
1106	44-45	-0.3024227139386326
1106	46-47	-0.3799069482151012
1106	48-49	-0.38223562112035836
1106	50-51	-0.4760406439999656
1106	52-53	-0.5825140695379147
1106	54-55	-0.5940757958386058
1106	56-57	-0.6250269614399784
1106	58-59	-0.6197798090262872
1106	60-61	-0.6099624653188549
1106	62-63	-0.6359991462962995
1106	64-65	-0.6090917500660389
1106	66-67	-0.5712244601838883
1106	68-69	-0.6553360999347291
1106	70-71	-0.6692816917075284
1106	72-73	-0.5925076762688697
1106	74-75	-0.5671771371018721
1106	76-77	-0.5980822352273911
1106	78-79	-0.539288765880638
1106	80-81	-0.36860277771762284
1106	82-83	-0.28969705838099813
1106	84-85	0.17747292406614434
1106	86-87	-0.2417070204371683
1106	88-89	-0.34869960106384923
1106	90-91	-0.3545161083720565
1106	92-93	-0.4866717041986739
1106	94-95	-0.4381481055137435
1106	96-97	-0.4551879873221658
1106	98-99	-0.4137710840795279
1106	100-101	-0.34677394093005987
1107	1	-0.5000701852665834
1107	2	-0.33868189198016196
1107	3	-0.3977415629139571
1107	4	-0.3193206271384028
1107	5	-0.44791708270970787
1107	6	-0.4887531341447229
1107	7	-0.35397910883686734
1107	8	-0.3352384303095519
1107	9	-0.473461452747884
1107	10-11	-0.6047451459801678
1107	12-13	-0.44670335367370484
1107	14-15	-0.6421190232427492
1107	16-17	-0.73728976203126
1107	18-19	-0.7943344092103501
1107	20-21	-0.8091172100227908
1107	22-23	-0.779493351202639
1107	24-25	-0.8303865765512057
1107	26-27	-0.8360775895084842
1107	28-29	-0.8504476111023465
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2308	48-49	-1.69454675200096
2308	50-51	-1.6379832985114646
2308	52-53	-1.7530539422237723
2308	54-55	-1.654493465111159
2308	56-57	-1.7038148645542677
2308	58-59	-1.6995365915374023
2308	60-61	-1.6932650143326171
2308	62-63	-1.7773980192768875
2308	64-65	-1.766036895320493
2308	66-67	-1.7017069002025202
2308	68-69	-1.6081366931728063
2308	70-71	-1.5098981255489115
2308	72-73	-1.7242239441154688
2308	74-75	-1.9234501113355371
2308	76-77	-1.7281639668563678
2308	78-79	-1.892056923766443
2308	80-81	-1.7282478535457244
2308	82-83	-1.534954690429572
2308	84-85	-1.2239174538177409
2308	86-87	-1.5642207613082313
2308	88-89	-1.9002289592370118
2308	90-91	-2.0182771011317335
2308	92-93	-2.0581657454794815
2308	94-95	-1.770096540591755
2308	96-97	-1.6308075270291198
2308	98-99	-2.0195319544542976
2308	100-101	-2.281731488429017
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1269832.0
3	438096.0
4	291992.0
5	339427.0
6	791628.0
7	716560.0
8	573872.0
9	866171.0
10	1348434.0
11	1384232.0
12	965514.0
13	657304.0
14	505502.0
15	457108.0
16	424536.0
17	400465.0
18	389858.0
19	390191.0
20	397114.0
21	417394.0
22	452857.0
23	501912.0
24	558935.0
25	628545.0
26	719144.0
27	817766.0
28	868690.0
29	1000137.0
30	1196564.0
31	1464386.0
32	1848623.0
33	2456306.0
34	3504683.0
35	5611167.0
36	1.1050562E7
37	2.1016506E7
38	1.3000192E7
39	404122.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.96469431130322	15.944791075023158	8.426819652318459	44.66369496135516
2	14.718370169726105	16.605979375244324	54.983242649660326	13.69240780536925
3	19.696331783867652	20.011903452811286	38.54058536637601	21.751179396945055
4	20.36518557176916	25.6183313822269	35.81951855501783	18.196964490986115
5	20.496067663786793	29.765377746756194	36.16560692497831	13.572947664478699
6	15.827533648083799	33.250263262397475	37.1991312069437	13.72307188257503
7	12.703791466503064	18.566303753506446	54.6285115688125	14.101393211177985
8	13.003609262977484	21.598388175292836	48.30891233494767	17.089090226782005
9	14.757212582215951	20.817109421870324	47.45498330629915	16.97069468961458
10-11	17.198099960423747	31.112447292763225	37.273811401647734	14.4156413451653
12-13	16.608167170414927	25.36508504301788	42.0631789567402	15.963568829826988
14-15	17.246069210382288	27.523800509472974	39.64625249095706	15.583877789187678
16-17	18.651608420707543	27.576718195171075	37.55987841250338	16.21179497161801
18-19	18.820031831982924	27.708749699271767	36.75233363674171	16.718884832003603
20-21	19.145840460360027	28.24087252915649	35.46934902086057	17.143937989622913
22-23	18.464221205885494	29.739658864449943	35.28383634588235	16.512283583782214
24-25	18.623868475894646	29.25335805030184	34.417166187230016	17.7056072865735
26-27	18.397819188800746	29.799539929015346	33.853090065985924	17.949550816197988
28-29	19.217235842235183	29.91410214452475	32.882061578484105	17.98660043475596
30-31	18.976276974419306	30.37279343291034	32.58151430894796	18.0694152837224
32-33	18.699008366395866	30.134348428849876	32.418176057845464	18.74846714690879
34-35	19.17777003916478	30.414171216700286	32.0212303243986	18.38682841973633
36-37	19.19254561449632	30.925074616097227	31.641899718344074	18.24048005106238
38-39	19.326125765074654	30.151952795642174	32.028311018009795	18.493610421273377
40-41	18.925734996866446	30.688394955525727	32.46893081135579	17.916939236252034
42-43	19.10398125709317	30.218248962560466	32.49517474448668	18.182595035859674
44-45	19.306695120839542	29.581445368876686	32.27973105954219	18.832128450741575
46-47	18.770971243134927	30.778489525277923	32.05530050242215	18.395238729164998
48-49	18.654426539219795	30.49764257072854	32.41931627109659	18.428614618955073
50-51	18.405648170912652	30.26704721552506	32.368430895936804	18.958873717625476
52-53	19.289199892155896	30.195226722074313	31.716000206802086	18.799573178967705
54-55	19.02427961979258	30.68829078146878	31.248285324584963	19.039144274153674
56-57	19.81791088652976	30.511434960571883	31.094638557936545	18.576015594961813
58-59	19.114994292071856	31.004058135632373	31.339339515263827	18.541608057031944
60-61	19.559982042553965	31.066698640437274	30.43880081337049	18.934518503638266
62-63	19.44460081234471	31.57571611729803	30.442812739425023	18.53687033093224
64-65	19.234693165981284	31.197490929843426	30.472037528097506	19.09577837607778
66-67	19.415891752184027	31.995633695625536	30.03844469261366	18.550029859576778
68-69	19.559115990009076	31.769176286721773	30.245064746645618	18.426642976623533
70-71	18.94481345001757	31.64518957090593	30.09979718439446	19.31019979468204
72-73	19.041432411149454	31.756677079049172	30.02698176003717	19.174908749764203
74-75	19.540298823917876	31.159841021705777	29.934077555810816	19.365782598565527
76-77	18.957730319031928	31.638372518065538	30.11084961809572	19.293047544806814
78-79	18.975378558650696	32.415596727799745	29.776689078727898	18.83233563482166
80-81	19.093306744165154	32.22282993481791	30.04776446270432	18.636098858312614
82-83	19.405322563862086	31.808284861572396	30.02274096536105	18.76365160920447
84-85	19.255807156764867	31.780894233373658	29.857864606735856	19.105434003125623
86-87	19.014405059877568	32.28598525964409	30.030592914772182	18.669016765706154
88-89	19.279543103246493	31.976144621554354	29.89308928055926	18.85122299463989
90-91	18.62124676775309	32.69357401442781	29.621273537161525	19.063905680657584
92-93	19.518681647846588	31.888307627983316	29.905687835227823	18.68732288894228
94-95	19.498211027212424	32.333488924404	29.050667738198673	19.117632310184902
96-97	18.9423874495171	32.798343566403894	29.418646184099305	18.840622799979702
98-99	19.735731839205215	32.17823453642155	29.050091434996972	19.035942189376264
100-101	19.614602485144044	33.384684297657415	28.104329044114035	18.896384173084506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20333.0
1	25089.0
2	27739.5
3	30740.0
4	43195.5
5	60499.5
6	80154.5
7	101533.0
8	126131.0
9	151518.0
10	177168.0
11	203352.0
12	229269.0
13	254082.5
14	281575.5
15	310476.0
16	337794.0
17	368538.5
18	406026.5
19	449007.0
20	501623.5
21	574691.5
22	677589.0
23	793986.5
24	924126.5
25	1112222.0
26	1307580.5
27	1544520.0
28	1793335.0
29	2041515.5
30	2313276.5
31	2470113.0
32	2565632.5
33	2742477.5
34	2985958.0
35	3228055.5
36	3460937.0
37	3541997.5
38	3596204.0
39	3545444.5
40	3554731.5
41	3628410.0
42	3195234.0
43	2701878.0
44	2442213.0
45	2272197.5
46	2160521.0
47	1983093.0
48	1828274.0
49	1691490.0
50	1455278.5
51	1219042.5
52	1058570.0
53	938019.0
54	815632.5
55	688846.0
56	600064.0
57	533014.0
58	468172.5
59	386433.5
60	284900.0
61	201179.0
62	152776.0
63	119408.5
64	84534.5
65	54389.5
66	34837.0
67	24531.5
68	17765.5
69	13067.5
70	12416.5
71	15133.0
72	16991.0
73	21581.5
74	19690.0
75	11096.5
76	8276.5
77	7523.0
78	5022.0
79	1771.0
80	920.5
81	551.5
82	368.5
83	276.0
84	221.5
85	182.0
86	123.0
87	74.0
88	66.0
89	59.0
90	38.0
91	22.5
92	20.0
93	17.0
94	9.0
95	7.5
96	5.5
97	7.5
98	7.5
99	4.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21747907064408079
2	0.0
3	0.0
4	0.0
5	5.491328437339781E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.5600364878806195E-5
34-35	0.0
36-37	2.6832627591546653E-5
38-39	0.0
40-41	3.744087570913487E-6
42-43	0.0016218139328006919
44-45	0.001541316050026052
46-47	0.0027163355326977347
48-49	0.0012748618178960423
50-51	0.003007750348633834
52-53	0.0017640892604954045
54-55	1.8096423259415185E-5
56-57	5.659812378030888E-4
58-59	6.208945221764865E-4
60-61	2.964069326973177E-4
62-63	1.553796341929097E-4
64-65	2.914148159360997E-4
66-67	5.572450334709572E-4
68-69	5.042037928830162E-4
70-71	0.002278901301496009
72-73	0.0025178988914393197
74-75	0.004611467858175111
76-77	0.002006206923414477
78-79	0.003966860781382839
80-81	0.004341269538474188
82-83	0.006592090183188346
84-85	0.007046996823054334
86-87	0.013177316205830018
88-89	0.02584169241444488
90-91	0.020256137773237114
92-93	0.004469192530480399
94-95	0.011563614462766304
96-97	0.013220373212895521
98-99	0.012978255549976449
100-101	0.005209897854926117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8.0126331E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.391746474595802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8726378357813	18.683604945118045
2	8.302260492432612	3.884087452100731
3	3.2146242592828402	2.2558702705268856
4	1.7063701666976288	1.5965991332481884
5	1.1620167624390352	1.3590800753102266
6	0.7611031514292367	1.0682119175549163
7	0.5908603277056999	0.9674878491311828
8	0.4691235141809603	0.877889464719398
9	0.3484857387585209	0.733652104594639
>10	2.7731189959929603	13.095602725467002
>50	0.40190805303499944	6.581982186278703
>100	0.32799597580207596	15.511550298643554
>500	0.03820315789814761	6.168029673349901
>1k	0.026700790717430214	12.093127815322882
>5k	0.0026902308531876373	4.315330403605865
>10k+	0.001900546993416761	10.807893685028205
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	248544	0.31019016707504055	TruSeq Adapter, Index 4 (100% over 50bp)
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	198517	0.24775501077167755	No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	193727	0.241776950950119	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	192510	0.24025809942551843	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG	150210	0.18746646467563827	TruSeq Adapter, Index 4 (100% over 49bp)
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	146897	0.1833317439681595	No Hit
GAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTC	116213	0.14503721629285635	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	106005	0.13229733431822804	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	102715	0.12819131828212627	No Hit
GTCGTATTATCCTATAGTGAGTCGTATTATCCTATAGTGAGTCGTATTAT	95481	0.11916307511946353	No Hit
AAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGA	89927	0.11223152099651236	No Hit
ATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTC	89342	0.11150142392018425	No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC	82296	0.10270781024529875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.24131642817889665	0.0	0.0	9.984233522435965E-6	0.0
2	0.24309736583346117	0.0	0.0	1.6224379473958444E-5	0.0
3	0.2433569559050445	0.0	0.0	1.8720437854567433E-5	0.0
4	0.256130534667811	0.0	0.0	1.996846704487193E-5	0.0
5	0.2573860520332573	0.0	0.0	2.246452542548092E-5	0.0
6	0.2581835426858619	1.2480291903044956E-6	0.0	2.4960583806089912E-5	0.0
7	0.26086430938663596	1.2480291903044956E-6	0.0	3.244875894791689E-5	0.0
8	0.2995919031909748	2.496058380608991E-6	0.0	3.369678813822138E-5	0.0
9	0.30026958304131013	3.744087570913487E-6	0.0	4.1184963280048356E-5	0.0
10-11	0.30837865270531356	3.744087570913487E-6	0.0	4.5553065446114086E-5	0.0
12-13	0.3138887015805079	3.744087570913487E-6	0.0	4.742510923157083E-5	0.0
14-15	0.3183229492936598	3.744087570913487E-6	0.0	5.179321139763656E-5	0.0
16-17	0.32149731153919925	3.744087570913487E-6	0.0	5.3665255183093306E-5	0.0
18-19	0.32429164889629103	3.744087570913487E-6	6.240145951522478E-7	5.8033357349159044E-5	0.0
20-21	0.33848236480464833	4.368102166065734E-6	1.2480291903044956E-6	6.864160546674725E-5	0.0
22-23	0.3533495125341506	4.992116761217982E-6	2.496058380608991E-6	7.300970763281299E-5	0.0
24-25	0.356519506677524	4.992116761217982E-6	2.496058380608991E-6	9.360218927283717E-5	0.0
26-27	0.35991913819191346	5.616131356370231E-6	3.120072975761239E-6	1.5849970716867094E-4	0.0
28-29	0.36342360415828845	6.240145951522478E-6	3.744087570913487E-6	2.053008018050895E-4	0.0
30-31	0.36738110472074403	7.488175141826974E-6	3.744087570913487E-6	2.1590904992267772E-4	0.0
32-33	0.37124749915230737	7.488175141826974E-6	3.744087570913487E-6	2.1590904992267772E-4	0.0
34-35	0.3756973472303381	1.0608248117588213E-5	3.744087570913487E-6	2.1715707911298222E-4	1.2480291903044956E-6
36-37	0.38007543362992624	1.372832109334945E-5	4.992116761217982E-6	2.271413126354182E-4	1.2480291903044956E-6
38-39	0.38454587418959696	1.372832109334945E-5	4.992116761217982E-6	2.2776532723057044E-4	1.2480291903044956E-6
40-41	0.38954672715514704	1.5600364878806195E-5	4.992116761217982E-6	2.3712554615785416E-4	1.2480291903044956E-6
42-43	0.3948446110679896	1.684839406911069E-5	4.992116761217982E-6	2.489818234657469E-4	1.2480291903044956E-6
44-45	0.4005699449785115	1.7472408664262937E-5	4.992116761217982E-6	2.6333415915424853E-4	1.2480291903044956E-6
46-47	0.4073111746499412	1.7472408664262937E-5	4.992116761217982E-6	3.007750348633834E-4	1.2480291903044956E-6
48-49	0.41452291132611574	1.8096423259415185E-5	4.992116761217982E-6	3.1013525379066715E-4	1.8720437854567434E-6
50-51	0.42228315683142914	1.934445244971968E-5	4.992116761217982E-6	3.107592683858194E-4	2.496058380608991E-6
52-53	0.4309332471494296	1.996846704487193E-5	4.992116761217982E-6	3.1263131217127614E-4	3.120072975761239E-6
54-55	0.4408575752707309	2.1216496235176423E-5	4.992116761217982E-6	3.1637539974218963E-4	3.744087570913487E-6
56-57	0.45215286345758177	2.1216496235176423E-5	4.992116761217982E-6	3.194954727179509E-4	3.744087570913487E-6
58-59	0.464445950982081	2.1216496235176423E-5	4.992116761217982E-6	3.288556916452346E-4	3.744087570913487E-6
60-61	0.4776257632462917	2.184051083032867E-5	4.992116761217982E-6	3.35095837596757E-4	3.744087570913487E-6
62-63	0.4929590498783727	2.246452542548092E-5	4.992116761217982E-6	3.488241586901065E-4	3.744087570913487E-6
64-65	0.5104601632140127	2.496058380608991E-5	4.992116761217982E-6	3.6754459654467393E-4	3.744087570913487E-6
66-67	0.528860481581267	3.120072975761239E-5	4.992116761217982E-6	3.868890489943936E-4	3.744087570913487E-6
68-69	0.5491815168723999	3.182474435276464E-5	4.992116761217982E-6	3.9250518035076386E-4	3.744087570913487E-6
70-71	0.5742693996559008	3.494481732852587E-5	4.992116761217982E-6	3.9500123873137285E-4	3.744087570913487E-6
72-73	0.6016792407479634	3.494481732852587E-5	4.992116761217982E-6	3.993693408974386E-4	3.744087570913487E-6
74-75	0.6332325387518367	3.556883192367812E-5	4.992116761217982E-6	4.237059101083762E-4	3.744087570913487E-6
76-77	0.672031270220023	3.619284651883037E-5	4.992116761217982E-6	5.104439388345387E-4	3.744087570913487E-6
78-79	0.7171712380041462	3.619284651883037E-5	4.992116761217982E-6	5.460127707582168E-4	3.744087570913487E-6
80-81	0.7696096804931702	3.619284651883037E-5	4.992116761217982E-6	5.865737194431129E-4	3.744087570913487E-6
82-83	0.831711613002722	3.619284651883037E-5	6.240145951522478E-6	6.090382448685938E-4	3.744087570913487E-6
84-85	0.907295380840538	3.619284651883037E-5	6.240145951522478E-6	6.17150434605573E-4	3.744087570913487E-6
86-87	1.0024114295212145	3.619284651883037E-5	6.240145951522478E-6	6.215185367716388E-4	3.744087570913487E-6
88-89	1.1185667542920443	3.7440875709134866E-5	6.240145951522478E-6	6.27134668128009E-4	3.744087570913487E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	71265	0.0	62.733692	1
GCGTGTT	90855	0.0	49.275433	2
GTTTTTT	1276445	0.0	46.082603	1
CCCGAAA	14910	0.0	43.387123	1
CGGTGGT	10575	0.0	41.547066	1
CCGAAAC	18930	0.0	33.721004	2
CGTGTTT	135100	0.0	33.461254	3
GTGTTTT	332870	0.0	31.064487	1
CGAAACC	21605	0.0	29.611824	3
CGGCCTT	9860	0.0	29.159433	1
CGAGCTA	17850	0.0	28.430702	12-13
CCGAGAT	10825	0.0	28.274967	1
CAAAACG	88585	0.0	28.250055	9
TCGTCTA	17675	0.0	28.162966	44-45
CCCAGAT	45820	0.0	27.613338	1
CGGAGAT	9760	0.0	27.556093	1
CTAACGA	16880	0.0	27.510191	94-95
TATGCGT	14500	0.0	27.4061	94-95
ACTCGTC	18740	0.0	26.49909	42-43
CTCGTAT	89025	0.0	26.43409	42-43
>>END_MODULE