FastQCFastQC Report
Fri 27 May 2016
SRR1233038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50899296
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1344130.2640763440028719No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC1252410.24605644840352997No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1210450.23781271945293703No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1198300.23542565303850174No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT841990.16542272018850712No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT817460.16060340009417812No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC740220.14542833755500273No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA698560.1372435485158773No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA655750.12883282314945968No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA638240.12539269698347105No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT596090.11711163942228198No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT563670.11074219965635675No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561640.11034337292209306No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC555560.10914885738301763No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG523900.10292873205947682No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT513560.10089726977756235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT316300.065.962471
GCGTGTT444950.046.280872
GTTTTTT6180400.038.292261
CGTGTTT645600.032.6323363
ACTCGTC441950.031.8142742-43
TCGTCTA455650.030.7189944-45
CTAACGA459100.028.93757294-95
TTAGGCA479550.028.7589728
CCTTTAG489100.028.5063045
TAGGCAT483750.028.264559
CAAAACG367500.027.0971859
CGCGCGT16150.026.2986491
GTCTATG548400.025.75519246-47
GTGGCAA557100.025.25454752-53
GCCTAAC537900.025.2398992-93
CCCGTAT58050.025.2378331
GCGCGTT15750.025.0565952
TTTAGGC587350.024.490037
CCCGAAA611850.024.2644791
ATGTGGC593100.023.76341850-51
Produced by FastQC (version 0.11.5)