##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233038_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50899296 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.875304306762906 31.0 30.0 34.0 25.0 34.0 2 30.043671782808154 31.0 31.0 34.0 25.0 34.0 3 30.046213939776298 31.0 30.0 34.0 25.0 34.0 4 33.392917497326486 37.0 35.0 37.0 26.0 37.0 5 33.27027601717713 37.0 35.0 37.0 26.0 37.0 6 33.389455622333166 37.0 35.0 37.0 26.0 37.0 7 33.43200953113379 37.0 35.0 37.0 26.0 37.0 8 33.34291886866176 37.0 35.0 37.0 26.0 37.0 9 34.81636003374192 39.0 35.0 39.0 27.0 39.0 10-11 34.721721769982835 39.0 35.0 39.0 26.5 39.0 12-13 34.62881397220111 39.0 35.0 39.0 26.5 39.0 14-15 35.523689581089684 39.0 35.0 41.0 25.0 41.0 16-17 35.30117896915509 39.0 34.5 41.0 25.0 41.0 18-19 35.01104787382521 39.0 34.0 41.0 24.0 41.0 20-21 34.815323545928806 39.0 34.0 41.0 20.5 41.0 22-23 34.629514011352924 39.0 34.0 41.0 18.5 41.0 24-25 34.36354827972473 39.0 34.0 41.0 18.0 41.0 26-27 34.12889016186 39.0 34.0 41.0 17.0 41.0 28-29 34.02697525521768 39.0 34.0 41.0 15.5 41.0 30-31 33.84938521546546 39.0 33.5 41.0 11.5 41.0 32-33 33.72020751524737 39.0 33.0 41.0 10.0 41.0 34-35 33.54802330664849 39.0 33.0 41.0 9.0 41.0 36-37 33.42022760982785 38.0 33.0 41.0 9.0 41.0 38-39 33.276921325198686 38.0 33.0 41.0 9.0 41.0 40-41 33.1436324973925 38.0 33.0 41.0 8.0 41.0 42-43 33.00524662462915 38.0 32.5 40.5 7.5 41.0 44-45 32.74389978792634 38.0 32.0 40.0 2.0 41.0 46-47 32.621791301396385 38.0 31.0 40.0 2.0 41.0 48-49 32.542997441457736 38.0 31.0 40.0 2.0 41.0 50-51 32.001327366099524 37.5 31.0 39.5 2.0 40.5 52-53 32.041299186141984 38.0 31.0 40.0 2.0 40.5 54-55 32.320139535918145 38.0 31.0 40.0 2.0 41.0 56-57 32.21714415264211 38.0 31.0 40.0 2.0 41.0 58-59 32.052116320037115 38.0 31.0 40.0 2.0 41.0 60-61 31.956800512525753 38.0 31.0 40.0 2.0 41.0 62-63 31.712571496077274 38.0 31.0 40.0 2.0 41.0 64-65 31.4266496239948 37.0 30.0 40.0 2.0 41.0 66-67 31.167198078731776 37.0 30.0 40.0 2.0 41.0 68-69 30.832480472814396 36.0 29.5 39.0 2.0 41.0 70-71 30.455038533342385 36.0 29.0 39.0 2.0 41.0 72-73 30.10059437757253 35.5 29.0 39.0 2.0 40.5 74-75 29.676689329848493 35.0 28.5 38.0 2.0 40.0 76-77 29.27176609672558 35.0 28.0 37.0 2.0 39.5 78-79 28.90238394456379 35.0 28.0 37.0 2.0 39.0 80-81 28.48711791612992 34.5 27.0 36.0 2.0 39.0 82-83 28.10308841992628 34.0 26.0 36.0 2.0 37.5 84-85 27.767539476380968 34.0 26.0 35.5 2.0 37.0 86-87 27.377109321904964 34.0 26.0 35.0 2.0 37.0 88-89 27.086485881847953 34.0 25.5 35.0 2.0 36.0 90-91 26.80300313976838 34.0 25.0 35.0 2.0 36.0 92-93 26.46870172821251 34.0 24.5 35.0 2.0 36.0 94-95 26.08547957126951 34.0 23.5 35.0 2.0 35.5 96-97 25.73458921710823 34.0 20.0 35.0 2.0 35.0 98-99 25.297221016573587 34.0 10.0 35.0 2.0 35.0 100-101 23.575661498343713 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1876408875606508 1101 2 1.510420736874778 1101 3 1.5847999260301293 1101 4 1.654788929135286 1101 5 1.6725439305735321 1101 6 1.4014885804126322 1101 7 1.6168432844618437 1101 8 1.7263697596915009 1101 9 1.676741656651977 1101 10-11 1.7845694167896085 1101 12-13 1.815471934196644 1101 14-15 1.9006946966088307 1101 16-17 1.9201862812392037 1101 18-19 2.0984842756404376 1101 20-21 2.116289339400325 1101 22-23 2.0131977648946844 1101 24-25 2.0468683223919513 1101 26-27 1.9450129387474604 1101 28-29 1.9705258331103366 1101 30-31 2.0156468798263845 1101 32-33 1.9469009105620856 1101 34-35 2.011180792879138 1101 36-37 1.8812716073330193 1101 38-39 1.8115936257720335 1101 40-41 1.9067672887329934 1101 42-43 1.9678018112403706 1101 44-45 1.9056528996441813 1101 46-47 1.9649926072119612 1101 48-49 2.016821007253597 1101 50-51 2.011824302537086 1101 52-53 1.9583170825532008 1101 54-55 2.0609687970032553 1101 56-57 2.1513592070667684 1101 58-59 2.2381233606056554 1101 60-61 2.2473041634161746 1101 62-63 2.2584507979050272 1101 64-65 2.2328859587730747 1101 66-67 2.2039883352664376 1101 68-69 2.1846678317381 1101 70-71 2.1814343537272016 1101 72-73 2.2006075563385927 1101 74-75 2.1613279564898313 1101 76-77 2.116443163219337 1101 78-79 2.1149489920318985 1101 80-81 2.0802744250232834 1101 82-83 1.9385487186441388 1101 84-85 2.0043124378698103 1101 86-87 2.0955680667384904 1101 88-89 2.1465116835929834 1101 90-91 2.113320494993232 1101 92-93 2.173752816974428 1101 94-95 2.1975360862965942 1101 96-97 2.2480489030806226 1101 98-99 2.2682883042752913 1101 100-101 2.2868255329240306 1102 1 1.2220372475102863 1102 2 1.5408702305812447 1102 3 1.5130846492375554 1102 4 1.5790292154715786 1102 5 1.6423867311786893 1102 6 1.5662197315252087 1102 7 1.6559661887030401 1102 8 1.7369129037288218 1102 9 1.8559324268099786 1102 10-11 1.8371578455695428 1102 12-13 1.7782969704786993 1102 14-15 2.030644999022762 1102 16-17 2.0271652648867544 1102 18-19 1.9793945582868488 1102 20-21 2.023504836059118 1102 22-23 1.8845527482202584 1102 24-25 1.8505722852217374 1102 26-27 1.8599015016440816 1102 28-29 1.8891597050528546 1102 30-31 1.8916456290724923 1102 32-33 1.8131936457430768 1102 34-35 1.7882366264667624 1102 36-37 1.836609074135147 1102 38-39 1.8598965553891134 1102 40-41 1.875474824267556 1102 42-43 1.8683585451605964 1102 44-45 1.8166293724594027 1102 46-47 1.9287782176906418 1102 48-49 1.9210482853487747 1102 50-51 1.8748365442793826 1102 52-53 1.903919395726959 1102 54-55 2.0034992179072617 1102 56-57 2.069828234522639 1102 58-59 2.2166256838105127 1102 60-61 2.1625586356042135 1102 62-63 2.126182639239886 1102 64-65 2.1148556592333705 1102 66-67 2.148321794730826 1102 68-69 2.0570138385959886 1102 70-71 2.1194205758690607 1102 72-73 2.0780595441978953 1102 74-75 2.060958523080828 1102 76-77 2.026569509282531 1102 78-79 2.047301148889005 1102 80-81 2.0867880021925984 1102 82-83 1.9906846485265497 1102 84-85 2.0766066639701926 1102 86-87 2.081167340638263 1102 88-89 2.0542688389774604 1102 90-91 2.09762005057463 1102 92-93 2.1031985770682624 1102 94-95 2.116006707967273 1102 96-97 2.2476719369021403 1102 98-99 2.2486368450603003 1102 100-101 2.224260677765912 1103 1 1.172400111549912 1103 2 1.4614299849955117 1103 3 1.4122105124022575 1103 4 1.4884703065288676 1103 5 1.5342336424051268 1103 6 1.4539615340135512 1103 7 1.4902813139990698 1103 8 1.5602817254929704 1103 9 1.596301116528572 1103 10-11 1.6294503630513262 1103 12-13 1.6006977451301339 1103 14-15 1.866074496195182 1103 16-17 1.8635208621459043 1103 18-19 1.9129782556954282 1103 20-21 1.9032456515306677 1103 22-23 1.7593674450807057 1103 24-25 1.7770102637087675 1103 26-27 1.765511015383943 1103 28-29 1.7874792531625658 1103 30-31 1.822541985660088 1103 32-33 1.7122550892005037 1103 34-35 1.8047724528487024 1103 36-37 1.7746161789460686 1103 38-39 1.8290511230254154 1103 40-41 1.8266695931260841 1103 42-43 1.857146309952313 1103 44-45 1.8017059386954557 1103 46-47 1.7862638054881117 1103 48-49 1.903390813897687 1103 50-51 1.8473764417493257 1103 52-53 1.8237436670777285 1103 54-55 1.9422817638120904 1103 56-57 1.944137696821734 1103 58-59 2.0960182469382573 1103 60-61 2.045772088909004 1103 62-63 1.954605053450642 1103 64-65 1.925307379720472 1103 66-67 1.9336684725026245 1103 68-69 1.9524724689130757 1103 70-71 1.97809222844295 1103 72-73 1.9798517004543434 1103 74-75 1.9647699254521385 1103 76-77 1.928825695236874 1103 78-79 1.9710738436253301 1103 80-81 1.998152897263882 1103 82-83 1.9252851382825646 1103 84-85 1.9405816615240248 1103 86-87 1.8695926866878665 1103 88-89 1.8987544111004588 1103 90-91 1.9973630367231436 1103 92-93 1.9684048222531132 1103 94-95 2.0496826413346163 1103 96-97 2.105064516891826 1103 98-99 2.1117704974878926 1103 100-101 2.170515909714858 1104 1 1.142133615390449 1104 2 1.3602011287309779 1104 3 1.334351672495238 1104 4 1.3749049482258329 1104 5 1.4975121971271648 1104 6 1.3968401761257354 1104 7 1.4947223145390538 1104 8 1.5709191990721223 1104 9 1.6342596625797015 1104 10-11 1.4855229783155508 1104 12-13 1.5423459627269693 1104 14-15 1.8309385934316893 1104 16-17 1.7760490947342973 1104 18-19 1.760142454212847 1104 20-21 1.7531604375341345 1104 22-23 1.668191285302541 1104 24-25 1.6935571042182005 1104 26-27 1.6890333490007237 1104 28-29 1.6855545641736285 1104 30-31 1.6531974379440086 1104 32-33 1.6835451909943515 1104 34-35 1.7291962320416658 1104 36-37 1.6466316949751558 1104 38-39 1.6804747543131526 1104 40-41 1.6842980426302887 1104 42-43 1.7151278698811936 1104 44-45 1.6678520823652434 1104 46-47 1.698286690649148 1104 48-49 1.7517472959154858 1104 50-51 1.629956420131471 1104 52-53 1.7250850320588071 1104 54-55 1.8954591398788239 1104 56-57 1.8094069868324354 1104 58-59 1.9415056418080354 1104 60-61 1.9248683455125217 1104 62-63 1.8466178514953242 1104 64-65 1.748325661613059 1104 66-67 1.8298906751655934 1104 68-69 1.8118691020752742 1104 70-71 1.8076291561609885 1104 72-73 1.8464647826279794 1104 74-75 1.8186241326845547 1104 76-77 1.8169031063378114 1104 78-79 1.8722792490776214 1104 80-81 1.8977718776112056 1104 82-83 1.9144928054443362 1104 84-85 1.860282890329028 1104 86-87 1.8206035327195558 1104 88-89 1.836218064188433 1104 90-91 1.9000450862439955 1104 92-93 1.8717842568602379 1104 94-95 1.878832692872738 1104 96-97 1.8533022582459218 1104 98-99 1.9214229976365012 1104 100-101 1.9586783762127915 1105 1 0.9092471959896642 1105 2 1.1527773720930092 1105 3 1.1116258270491635 1105 4 1.1573848734223233 1105 5 1.2449506288900594 1105 6 1.1581416570489296 1105 7 1.1826176041802228 1105 8 1.2572854600953676 1105 9 1.3512314538007857 1105 10-11 1.267377056643987 1105 12-13 1.2656176690910712 1105 14-15 1.525015871534812 1105 16-17 1.4364062069806849 1105 18-19 1.4738143852945527 1105 20-21 1.4855115158077439 1105 22-23 1.4009076112560592 1105 24-25 1.460981381648331 1105 26-27 1.3887945056763868 1105 28-29 1.375166432010694 1105 30-31 1.4357167716414807 1105 32-33 1.3720706378837306 1105 34-35 1.3237582969937876 1105 36-37 1.249720592593306 1105 38-39 1.275717419164721 1105 40-41 1.3302529960207607 1105 42-43 1.321807585903791 1105 44-45 1.2637086636545263 1105 46-47 1.3230702394232239 1105 48-49 1.2674497342341056 1105 50-51 1.262191160469527 1105 52-53 1.3121427327769979 1105 54-55 1.3980191673562814 1105 56-57 1.467027202632707 1105 58-59 1.5460312704849883 1105 60-61 1.5098901658391384 1105 62-63 1.4666671803553157 1105 64-65 1.4198666277581324 1105 66-67 1.4726596913299588 1105 68-69 1.536058660283718 1105 70-71 1.5580521457401169 1105 72-73 1.573207840976007 1105 74-75 1.551109771314234 1105 76-77 1.5822748352005078 1105 78-79 1.5239475861288199 1105 80-81 1.4990827157802222 1105 82-83 1.4475613936634097 1105 84-85 1.4695767608370183 1105 86-87 1.4922578812646314 1105 88-89 1.4675001344633571 1105 90-91 1.5372718108646097 1105 92-93 1.5416177930988084 1105 94-95 1.5368659066396013 1105 96-97 1.540039983779316 1105 98-99 1.4787321158024618 1105 100-101 1.498583669923164 1106 1 0.8212695062806823 1106 2 1.0706523927544218 1106 3 1.0533692591054802 1106 4 1.1634755682168105 1106 5 1.21719644836692 1106 6 1.117957441100721 1106 7 1.212642901633778 1106 8 1.2227694967103204 1106 9 1.2911185754586256 1106 10-11 1.1918732736020061 1106 12-13 1.1580727438047802 1106 14-15 1.2763855709417697 1106 16-17 1.3054294202075596 1106 18-19 1.3308769197831438 1106 20-21 1.224181424322424 1106 22-23 1.2456050860661065 1106 24-25 1.1241424166547702 1106 26-27 1.134903239318362 1106 28-29 1.0569051054179752 1106 30-31 1.1481916640515735 1106 32-33 1.1687764137444674 1106 34-35 1.206696745214792 1106 36-37 1.1533799590804534 1106 38-39 1.1304746560344938 1106 40-41 1.1750701865905029 1106 42-43 1.2283440746922594 1106 44-45 1.188588976055371 1106 46-47 1.2669749200048486 1106 48-49 1.2282935287189503 1106 50-51 1.098871921846957 1106 52-53 1.1102252757600581 1106 54-55 1.2044301384626621 1106 56-57 1.2651998694606092 1106 58-59 1.365277830164004 1106 60-61 1.3185460513036702 1106 62-63 1.3987132202730521 1106 64-65 1.3737923981647882 1106 66-67 1.3737893865312216 1106 68-69 1.1895528277411138 1106 70-71 1.2797999883577056 1106 72-73 1.2688691559740057 1106 74-75 1.3024100234041782 1106 76-77 1.2799642058503835 1106 78-79 1.3719745424789878 1106 80-81 1.392122882986346 1106 82-83 1.3129177576864386 1106 84-85 1.2874330674411567 1106 86-87 1.347947742782818 1106 88-89 1.2861368072821158 1106 90-91 1.321562280170884 1106 92-93 1.3691362038229116 1106 94-95 1.3045912172116232 1106 96-97 1.3755936078104796 1106 98-99 1.4188502346025054 1106 100-101 1.3274771841226283 1107 1 0.7732418129947582 1107 2 1.0039464276203844 1107 3 0.9037134636842836 1107 4 0.9953739703178641 1107 5 0.96872072395103 1107 6 0.9322455049445821 1107 7 1.0121804751975034 1107 8 1.106474508945169 1107 9 1.1482463363038278 1107 10-11 1.1102135909682644 1107 12-13 1.0796954292477636 1107 14-15 1.1999839623051116 1107 16-17 1.158520967985666 1107 18-19 1.1503725648740968 1107 20-21 1.1771890942359775 1107 22-23 1.1257923197903494 1107 24-25 1.03141347435011 1107 26-27 0.9663834126171409 1107 28-29 1.0219179587147167 1107 30-31 1.058637343045092 1107 32-33 1.0573791640955221 1107 34-35 1.0779798973424093 1107 36-37 0.9957620476251492 1107 38-39 0.8950530482710377 1107 40-41 0.9302841948431535 1107 42-43 0.9244148031831259 1107 44-45 0.9218605423821771 1107 46-47 0.9512094532712467 1107 48-49 0.9437473241986254 1107 50-51 0.9199469655600225 1107 52-53 0.8932634273842837 1107 54-55 1.0953532450551648 1107 56-57 1.0641179582698683 1107 58-59 1.1471100348828571 1107 60-61 1.154708749519557 1107 62-63 1.1407086286501809 1107 64-65 1.125128826458429 1107 66-67 1.09557856137463 1107 68-69 1.0853413281644215 1107 70-71 1.1075055209424427 1107 72-73 1.0826598437883739 1107 74-75 1.1123142804375377 1107 76-77 1.0936909194677824 1107 78-79 1.0889633635208114 1107 80-81 1.121188785429208 1107 82-83 1.0752271814205976 1107 84-85 1.088317278441668 1107 86-87 1.111761300129185 1107 88-89 1.1216103197621408 1107 90-91 1.125416632400082 1107 92-93 1.1151461039225623 1107 94-95 1.129961916365179 1107 96-97 1.1434452763690857 1107 98-99 1.183230211533676 1107 100-101 1.1308504834270394 1108 1 0.6286310329785785 1108 2 0.8894765128042721 1108 3 0.8043306525200116 1108 4 0.877475829143421 1108 5 0.9471007061145826 1108 6 0.8739157300213449 1108 7 0.9212743859189132 1108 8 0.9340000696884942 1108 9 0.9012802175088126 1108 10-11 0.9331410081357845 1108 12-13 0.8058489266931872 1108 14-15 0.9940925100459381 1108 16-17 0.8678297361364784 1108 18-19 0.9700958794333516 1108 20-21 0.9265198068737419 1108 22-23 0.7576282982575151 1108 24-25 0.8802030690381386 1108 26-27 0.819011167767961 1108 28-29 0.8989746757657926 1108 30-31 0.8799872156996003 1108 32-33 0.8950739898539481 1108 34-35 0.8694640371491147 1108 36-37 0.8871177555488714 1108 38-39 0.8742201150860822 1108 40-41 0.9002417139866168 1108 42-43 0.8183981714500632 1108 44-45 0.9233955467707062 1108 46-47 0.8819641479422913 1108 48-49 0.8841408031696574 1108 50-51 0.764905261782495 1108 52-53 0.7098166517272944 1108 54-55 0.8356208813282393 1108 56-57 0.805574372927218 1108 58-59 0.9538624539509613 1108 60-61 0.8398559871504858 1108 62-63 0.9118394633618436 1108 64-65 0.8229341286625314 1108 66-67 0.8276555014152436 1108 68-69 0.8556999991980554 1108 70-71 0.9211918573377567 1108 72-73 0.7750491973954539 1108 74-75 0.8466530827583583 1108 76-77 0.8413641963431289 1108 78-79 0.8779923565066099 1108 80-81 0.7890103609157286 1108 82-83 0.7104906466345717 1108 84-85 0.7827580679995343 1108 86-87 0.7741471961769903 1108 88-89 0.8747699941815874 1108 90-91 0.8982711179950158 1108 92-93 0.8174732094466783 1108 94-95 0.7501862952374694 1108 96-97 0.7931239498383498 1108 98-99 0.7527892532724216 1108 100-101 0.6885882036962911 1109 1 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2.246999236182468 2204 54-55 2.178225519199003 2204 56-57 2.2409038436833484 2204 58-59 2.1536586334426318 2204 60-61 2.1631943487592054 2204 62-63 2.186094021184516 2204 64-65 2.2081567026907365 2204 66-67 2.1636898126377773 2204 68-69 2.1402394006145578 2204 70-71 2.153083465864629 2204 72-73 2.172379029977332 2204 74-75 2.129339316971354 2204 76-77 2.135038629715851 2204 78-79 2.1038113622528343 2204 80-81 2.1975109870769423 2204 82-83 2.1407471956408237 2204 84-85 2.0940811184312516 2204 86-87 2.091036055402956 2204 88-89 2.1041784024680297 2204 90-91 2.1319986786830327 2204 92-93 2.1668303593427005 2204 94-95 2.167279503842746 2204 96-97 2.2258497066235705 2204 98-99 2.2836989410435784 2204 100-101 2.1373414526924925 2205 1 1.2316865305406033 2205 2 1.3282666184723482 2205 3 1.2506454250517223 2205 4 1.2044573420187419 2205 5 1.3075121207040539 2205 6 1.2217540146149801 2205 7 1.325506659471678 2205 8 1.3106814065011179 2205 9 1.437433406475762 2205 10-11 1.4702619567664001 2205 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1.6829358917214812 2205 80-81 1.71936716769115 2205 82-83 1.6860527769864255 2205 84-85 1.7141540774147082 2205 86-87 1.7158686767611648 2205 88-89 1.7495468664824259 2205 90-91 1.7530817706442043 2205 92-93 1.700673905523196 2205 94-95 1.6992612794257234 2205 96-97 1.7617364444933123 2205 98-99 1.7813355471715688 2205 100-101 1.6501334739936553 2206 1 1.2852561963066371 2206 2 1.4043284263316274 2206 3 1.2079040699031687 2206 4 1.1919047462789152 2206 5 1.2851103555163021 2206 6 1.1669068106094969 2206 7 1.3303537888248371 2206 8 1.3867776378830001 2206 9 1.3833816692604657 2206 10-11 1.3910363249520046 2206 12-13 1.3637426579558678 2206 14-15 1.640532958941833 2206 16-17 1.6510970939392848 2206 18-19 1.6938225809030243 2206 20-21 1.6717780370575497 2206 22-23 1.6210021948800062 2206 24-25 1.6557787021939632 2206 26-27 1.5799909829829417 2206 28-29 1.6470954190954714 2206 30-31 1.6682514371242902 2206 32-33 1.6581116080142024 2206 34-35 1.6766913997367396 2206 36-37 1.6864845885328137 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-5.7912813146848094 2316 2 -7.042845912330019 2316 3 -4.6880915283941675 2316 4 -4.562738874022859 2316 5 -4.458925777555951 2316 6 -3.6185592507243847 2316 7 -5.1299553049389495 2316 8 -4.030605102761271 2316 9 -3.5969999187918376 2316 10-11 -5.383316286964359 2316 12-13 -4.866633438153105 2316 14-15 -6.346473984777859 2316 16-17 -4.743748245393267 2316 18-19 -6.9999046426903035 2316 20-21 -6.432506799090135 2316 22-23 -4.774651757118278 2316 24-25 -4.748046217517199 2316 26-27 -4.323333518999966 2316 28-29 -4.3553678901557475 2316 30-31 -3.9826560034674756 2316 32-33 -4.192365777289883 2316 34-35 -4.291613276028379 2316 36-37 -4.151901488622197 2316 38-39 -4.147550080491218 2316 40-41 -4.235158077806702 2316 42-43 -4.3745879031016415 2316 44-45 -4.42271903434186 2316 46-47 -4.115976548899528 2316 48-49 -4.437943841961715 2316 50-51 -4.765423405740293 2316 52-53 -4.562857433458582 2316 54-55 -4.853964523734746 2316 56-57 -4.896198273753157 2316 58-59 -5.1066470331256895 2316 60-61 -5.103266087290528 2316 62-63 -5.047120588356819 2316 64-65 -4.742920408978023 2316 66-67 -4.642957695036813 2316 68-69 -4.652343517931708 2316 70-71 -4.901746895394478 2316 72-73 -4.4332088763041355 2316 74-75 -4.437877648960722 2316 76-77 -4.331453022257101 2316 78-79 -4.28138563249734 2316 80-81 -4.291164306863077 2316 82-83 -4.304159130631003 2316 84-85 -4.107335332552914 2316 86-87 -4.1055145061296585 2316 88-89 -4.176050472276312 2316 90-91 -4.038681768573646 2316 92-93 -4.144421914543653 2316 94-95 -4.088408179053918 2316 96-97 -4.096446811416566 2316 98-99 -4.101738784944704 2316 100-101 -3.9638970205942776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1011545.0 3 269751.0 4 252097.0 5 258309.0 6 306581.0 7 398958.0 8 502698.0 9 617895.0 10 657605.0 11 540082.0 12 431921.0 13 365528.0 14 330280.0 15 310871.0 16 305571.0 17 307011.0 18 318310.0 19 336107.0 20 362732.0 21 397649.0 22 438837.0 23 488151.0 24 542748.0 25 603333.0 26 673958.0 27 758576.0 28 862764.0 29 989485.0 30 1149035.0 31 1355648.0 32 1623654.0 33 1994702.0 34 2533517.0 35 3372253.0 36 4749549.0 37 7756445.0 38 9882980.0 39 2835785.0 40 6375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.647906869017202 15.063029003844461 12.656160283331358 45.632903843806986 2 14.667059238275213 18.4416261878113 52.786606675060256 14.104707898853233 3 19.474445265290267 22.605906703516617 35.5044347258349 22.415213305358215 4 21.18682664454007 30.0926005959382 30.613876509949055 18.106696249572675 5 20.874090784208843 32.52956022986757 31.454492993327786 15.1418559925958 6 15.82982928156611 35.728962289901126 32.41414566135755 16.02706276717521 7 13.318308390508186 19.431799584017558 49.83735325982169 17.41253876565257 8 14.028225019983894 21.522151656885452 42.848806872709055 21.600816450421597 9 15.858659475250578 22.813065269638663 41.15430584163029 20.173969413480467 10-11 19.030530820424357 33.55435295187704 30.822421863096473 16.592694364602124 12-13 17.896789299804723 26.527158340430173 37.36431372515852 18.21173863460659 14-15 18.81162106105696 27.905925257168064 35.8815709476267 17.40088273414828 16-17 19.5607918443266 27.660074651470513 34.341812509223004 18.43732099497988 18-19 19.54767302445487 28.34992754576377 33.58203413897287 18.52036529080849 20-21 19.939465635110636 27.7808926185049 32.97713191245445 19.302509833930014 22-23 19.529188612511224 29.334908634871105 33.46525819878657 17.670644553831096 24-25 19.170829956076215 29.328745338637702 32.48925706690437 19.011167638381714 26-27 19.343312646992544 29.83812279585556 32.13122567203497 18.687338885116922 28-29 19.652588941074896 29.3701617593607 31.946404209004186 19.03084509056022 30-31 18.916658750365674 29.789373738527026 31.936383746233098 19.357583764874196 32-33 19.00648242568857 29.39373324604288 32.28697951551279 19.312804812755758 34-35 19.409975531000732 30.499162351792414 30.788252007988948 19.3026101092179 36-37 19.76523697475401 30.19675725182292 31.28566296440587 18.752342809017204 38-39 19.50360328218993 29.658433710996423 31.539917315670756 19.298045691142892 40-41 19.7892508500258 29.885335291725628 30.91129277695949 19.414121081289085 42-43 19.540758461168103 30.129260855028583 31.238368388372823 19.09161229543049 44-45 19.751163036265947 29.77247945326255 31.15827722532416 19.318080285147346 46-47 19.497159332916 29.873408517988253 31.22263651386245 19.406795635233294 48-49 19.634655675731697 29.429408599485896 30.940287892144745 19.995647832637662 50-51 19.039100729943886 29.903209251193864 31.88616584471341 19.17152417414884 52-53 20.1777222214296 29.60090260751806 30.86859441799108 19.352780753061257 54-55 19.52839901763242 30.114150597601625 30.630948708324386 19.726501676441572 56-57 20.392757394366352 29.74421264033728 30.311675251475044 19.55135471382133 58-59 19.269957471343105 30.571242973262365 30.461240990157705 19.697558565236832 60-61 19.708519912893706 30.361039430369836 29.826698788840005 20.103741867896456 62-63 19.519653767708668 30.81074602916738 30.223784492030497 19.445815711093452 64-65 19.920938780636938 30.175946584656277 30.161611116267757 19.74150351843903 66-67 19.886267861599126 30.52473838276623 29.735722741988894 19.853271013645745 68-69 20.087694054865143 30.348673213873305 30.218270153533172 19.345362577728377 70-71 19.768327667223666 30.59117817727188 29.86950532868689 19.770988826817565 72-73 19.741102163691764 30.499947548917227 29.996927696516856 19.762022590874157 74-75 19.63351743976657 29.877439570241428 31.194307407221867 19.294735582770137 76-77 19.541917978261058 30.402320240866505 30.414206317625357 19.64155546324708 78-79 19.701683734401897 30.65141949270318 30.01699239245839 19.629904380436535 80-81 19.848903877893683 30.48947606494783 30.163172323185865 19.498447733972625 82-83 20.144473138727264 30.60288431790325 29.71759081646767 19.535051726901813 84-85 19.737922618620342 30.3390762495784 29.86204021188578 20.060960919915473 86-87 19.706741984291593 31.20028691429378 29.653460542704636 19.439510558709998 88-89 19.627020502652755 30.47808835671166 30.340323453532996 19.554567687102587 90-91 19.70831296986674 31.133289196855955 29.752076483252083 19.406321350025223 92-93 20.158262706548598 30.4298926564707 29.71327140708628 19.69857322989443 94-95 20.014303458156867 30.704471280455163 29.567614674688684 19.713610586699282 96-97 20.003630537362305 30.672519187296544 29.54331291549408 19.780537359847074 98-99 20.264760952171077 30.53933505686624 29.326768516657296 19.869135474305388 100-101 21.09733526347918 31.438487332043323 28.127743676757483 19.336433727720014 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24315.0 1 29814.0 2 29594.5 3 25393.5 4 28631.0 5 32077.0 6 33831.0 7 35469.0 8 38350.0 9 41618.0 10 45639.5 11 50215.5 12 55851.0 13 61820.0 14 68646.0 15 77585.0 16 88149.0 17 100053.5 18 114725.0 19 132056.0 20 154984.5 21 186274.5 22 223261.5 23 269354.0 24 336133.5 25 429918.5 26 577239.5 27 795679.0 28 943420.0 29 1044393.0 30 1228395.5 31 1444973.5 32 1634783.5 33 1816424.0 34 2073714.0 35 2366017.5 36 2585604.5 37 2830698.5 38 2946557.0 39 2513519.0 40 2228417.0 41 2193528.5 42 1896724.5 43 1682724.5 44 1577034.0 45 1470440.5 46 1350857.5 47 1262241.0 48 1214813.0 49 1187386.0 50 1101610.5 51 953606.5 52 837876.5 53 749083.0 54 677237.0 55 608787.0 56 535360.5 57 452411.5 58 372518.0 59 292651.5 60 211873.0 61 150432.0 62 106575.5 63 75069.5 64 52170.0 65 36171.0 66 25572.5 67 17965.0 68 12463.0 69 8611.5 70 6768.0 71 6442.5 72 5793.5 73 6011.0 74 5952.0 75 4097.5 76 3261.0 77 3128.5 78 2833.0 79 2151.0 80 1593.0 81 1232.5 82 964.0 83 715.5 84 629.0 85 614.0 86 548.0 87 397.0 88 251.5 89 188.5 90 141.5 91 112.0 92 80.5 93 57.5 94 41.0 95 25.0 96 13.5 97 9.5 98 6.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6696084755278344 2 0.3217215420818394 3 0.32824226095386466 4 0.3304505429701818 5 0.31535996097077645 6 0.22358266016095785 7 0.1105810972316788 8 0.1269388873276361 9 0.12039262782730827 10-11 0.11964409095167053 12-13 0.11508803579522986 14-15 0.12852437094611288 16-17 0.1146744740830993 18-19 0.131366257010706 20-21 0.13587614256983044 22-23 0.11441415614078435 24-25 0.12120010461441352 26-27 0.11366954859257777 28-29 0.11620396478568192 30-31 0.12842810242404923 32-33 0.11604875635215073 34-35 0.13768756251559944 36-37 0.13141144427616444 38-39 0.12555674640372236 40-41 0.13455195922552643 42-43 0.09874203368156606 44-45 0.34752543532232744 46-47 0.10215563688739428 48-49 0.08678803730409158 50-51 0.0906505661689309 52-53 0.23265645953138525 54-55 0.2800264663778454 56-57 0.1840064742742218 58-59 0.26117944735424237 60-61 0.07192732095940974 62-63 0.058672717202218276 64-65 0.10487669613347894 66-67 0.1021919831661326 68-69 0.08548448292880122 70-71 0.1115899520496315 72-73 0.13945968918705673 74-75 0.13023854003796045 76-77 0.12280130554261498 78-79 0.10588653328328942 80-81 0.09682157489958211 82-83 0.10322932560796125 84-85 0.10048469039728959 86-87 0.12418442879838652 88-89 0.12601844237688475 90-91 0.15945996581170788 92-93 0.16399244500356153 94-95 0.12581117035489056 96-97 0.10379023709876066 98-99 0.13064620775894425 100-101 0.12730136778316148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.0899296E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.36602967860904 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34080689244004 21.93851623678525 2 8.6620884360945 4.914181153139856 3 3.6610336994786774 3.1154697172140002 4 2.1120309803602226 2.396397338841593 5 1.3899347314895185 1.9713464922380586 6 1.0081984202776917 1.7159151786914253 7 0.7390485238207002 1.467471065244113 8 0.5998203680953648 1.3611617890581853 9 0.48389259221417547 1.2353500468805727 >10 3.412461495635951 18.90710076968555 >50 0.3531568412402043 6.890852875102309 >100 0.20421887747938072 10.761434397425464 >500 0.016492037120773243 3.226174511073385 >1k 0.013687187832154283 8.008522669964394 >5k 0.0018659979577795936 3.6612559859103855 >10k+ 0.0012629184631205062 8.428849772745611 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 134413 0.2640763440028719 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 125241 0.24605644840352997 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 121045 0.23781271945293703 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 119830 0.23542565303850174 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 84199 0.16542272018850712 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 81746 0.16060340009417812 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 74022 0.14542833755500273 No Hit CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA 69856 0.1372435485158773 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA 65575 0.12883282314945968 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 63824 0.12539269698347105 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 59609 0.11711163942228198 No Hit GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT 56367 0.11074219965635675 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56164 0.11034337292209306 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 55556 0.10914885738301763 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 52390 0.10292873205947682 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 51356 0.10089726977756235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03277452010338218 0.0 0.0 1.1787982293507557E-5 0.0 2 0.03302010306783025 3.929327431169185E-6 0.0 1.1787982293507557E-5 0.0 3 0.03315173553677442 5.8939911467537785E-6 0.0 1.571730972467674E-5 1.9646637155845927E-6 4 0.04100646107168162 5.8939911467537785E-6 1.9646637155845927E-6 1.9646637155845928E-5 1.9646637155845927E-6 5 0.04132866592103749 5.8939911467537785E-6 1.9646637155845927E-6 2.161130087143052E-5 1.9646637155845927E-6 6 0.041701952026998564 5.8939911467537785E-6 1.9646637155845927E-6 2.75052920181843E-5 1.9646637155845927E-6 7 0.042287421814242776 5.8939911467537785E-6 1.9646637155845927E-6 4.125793802727645E-5 1.9646637155845927E-6 8 0.04898299575695507 5.8939911467537785E-6 1.9646637155845927E-6 4.322260174286104E-5 1.9646637155845927E-6 9 0.04930520060631094 5.8939911467537785E-6 1.9646637155845927E-6 5.108125660519941E-5 3.929327431169185E-6 10-11 0.05126789965817995 9.823318577922964E-6 1.9646637155845927E-6 5.3045920320784E-5 3.929327431169185E-6 12-13 0.053564591541698334 1.1787982293507557E-5 1.9646637155845927E-6 5.697524775195319E-5 3.929327431169185E-6 14-15 0.056267968814342734 1.1787982293507557E-5 1.9646637155845927E-6 6.0904575183122375E-5 3.929327431169185E-6 16-17 0.05887704222863908 1.2770314151299854E-5 2.946995573376889E-6 6.483390261429156E-5 3.929327431169185E-6 18-19 0.06193798829751987 1.4734977866884446E-5 4.911659288961482E-6 8.05512123389683E-5 3.929327431169185E-6 20-21 0.06528381060516043 1.669964158246904E-5 5.8939911467537785E-6 9.233919463247586E-5 3.929327431169185E-6 22-23 0.0687308130941536 1.7681973440261336E-5 5.8939911467537785E-6 1.0118018135260652E-4 3.929327431169185E-6 24-25 0.07178095351259868 1.7681973440261336E-5 5.8939911467537785E-6 1.1100349993052949E-4 3.929327431169185E-6 26-27 0.07464052155063206 1.7681973440261336E-5 5.8939911467537785E-6 1.355617963753369E-4 3.929327431169185E-6 28-29 0.07776335452655377 1.9646637155845928E-5 5.8939911467537785E-6 1.4931444238442906E-4 3.929327431169185E-6 30-31 0.08106202490502029 2.2593632729222815E-5 5.8939911467537785E-6 1.630670883935212E-4 5.8939911467537785E-6 32-33 0.08416619357564395 2.3575964587015114E-5 5.8939911467537785E-6 1.6699641582469037E-4 5.8939911467537785E-6 34-35 0.08771437624598973 2.3575964587015114E-5 5.8939911467537785E-6 1.699434113980673E-4 5.8939911467537785E-6 36-37 0.09120165434115238 2.3575964587015114E-5 5.8939911467537785E-6 1.8369605740715944E-4 5.8939911467537785E-6 38-39 0.09491486876360726 2.3575964587015114E-5 5.8939911467537785E-6 1.895900485539132E-4 7.85865486233837E-6 40-41 0.0988245495576206 2.5540628302599706E-5 5.8939911467537785E-6 1.974487034162516E-4 7.85865486233837E-6 42-43 0.10302500058154046 2.5540628302599706E-5 5.8939911467537785E-6 2.210246680032667E-4 1.1787982293507557E-5 44-45 0.10758694972912788 2.75052920181843E-5 5.8939911467537785E-6 2.3281265029677424E-4 1.669964158246904E-5 46-47 0.11334144975207516 2.8487623875976596E-5 5.8939911467537785E-6 2.6621193346171236E-4 1.7681973440261336E-5 48-49 0.118487886355049 2.9469955733768892E-5 5.8939911467537785E-6 2.7701758389742754E-4 1.7681973440261336E-5 50-51 0.12454690925391187 3.143461944935348E-5 5.8939911467537785E-6 2.8094691132859675E-4 2.161130087143052E-5 52-53 0.13122971288247287 3.241695130714578E-5 5.8939911467537785E-6 2.8487623875976596E-4 2.161130087143052E-5 54-55 0.1387887565281846 3.634627873831497E-5 5.8939911467537785E-6 2.9175256176431204E-4 2.161130087143052E-5 56-57 0.14668768699669243 3.9293274311691856E-5 5.8939911467537785E-6 3.01575880342235E-4 2.161130087143052E-5 58-59 0.15544517550891077 3.9293274311691856E-5 5.8939911467537785E-6 3.18275521924704E-4 2.161130087143052E-5 60-61 0.16511623264887593 4.125793802727645E-5 7.85865486233837E-6 3.271165086448347E-4 2.161130087143052E-5 62-63 0.17618907734991068 4.322260174286104E-5 7.85865486233837E-6 3.34975163507173E-4 2.161130087143052E-5 64-65 0.18814602072295852 4.420493360065334E-5 7.85865486233837E-6 3.6051579180977275E-4 2.3575964587015114E-5 66-67 0.2025470057581936 4.518726545844564E-5 7.85865486233837E-6 3.6935677852990346E-4 2.5540628302599706E-5 68-69 0.2187142234737392 4.518726545844564E-5 7.85865486233837E-6 3.762331015344495E-4 2.5540628302599706E-5 70-71 0.23729404823202271 4.518726545844564E-5 7.85865486233837E-6 3.791800971078264E-4 2.5540628302599706E-5 72-73 0.25726780189651344 4.8134261031822524E-5 7.85865486233837E-6 3.840917563967879E-4 2.5540628302599706E-5 74-75 0.2806610527579792 4.911659288961482E-5 7.85865486233837E-6 3.9489740683250313E-4 2.5540628302599706E-5 76-77 0.30841192774061155 4.911659288961482E-5 7.85865486233837E-6 4.400846722909488E-4 2.5540628302599706E-5 78-79 0.3397984129289332 5.206358846299171E-5 7.85865486233837E-6 4.567843138734178E-4 2.5540628302599706E-5 80-81 0.37486176626097145 5.40282521785763E-5 7.85865486233837E-6 4.7053695988251E-4 2.5540628302599706E-5 82-83 0.4168829761417525 5.5992915894160896E-5 7.85865486233837E-6 4.8428960589160213E-4 2.5540628302599706E-5 84-85 0.46706638142892976 5.697524775195319E-5 7.85865486233837E-6 4.931305926117327E-4 2.5540628302599706E-5 86-87 0.5279748859394833 6.0904575183122375E-5 7.85865486233837E-6 5.078655704786172E-4 2.6522960160392005E-5 88-89 0.5986035641828916 6.286923889870697E-5 7.85865486233837E-6 5.13759561625371E-4 2.8487623875976596E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 31630 0.0 65.96247 1 GCGTGTT 44495 0.0 46.28087 2 GTTTTTT 618040 0.0 38.29226 1 CGTGTTT 64560 0.0 32.632336 3 ACTCGTC 44195 0.0 31.81427 42-43 TCGTCTA 45565 0.0 30.71899 44-45 CTAACGA 45910 0.0 28.937572 94-95 TTAGGCA 47955 0.0 28.758972 8 CCTTTAG 48910 0.0 28.506304 5 TAGGCAT 48375 0.0 28.26455 9 CAAAACG 36750 0.0 27.097185 9 CGCGCGT 1615 0.0 26.298649 1 GTCTATG 54840 0.0 25.755192 46-47 GTGGCAA 55710 0.0 25.254547 52-53 GCCTAAC 53790 0.0 25.23989 92-93 CCCGTAT 5805 0.0 25.237833 1 GCGCGTT 1575 0.0 25.056595 2 TTTAGGC 58735 0.0 24.49003 7 CCCGAAA 61185 0.0 24.264479 1 ATGTGGC 59310 0.0 23.763418 50-51 >>END_MODULE