FastQCFastQC Report
Fri 27 May 2016
SRR1233038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50899296
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1270750.24965964165791213No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1215020.2387105707709592No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA874980.1719041457862207No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC806070.15836564812212725No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT757940.14890972165901864No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA606070.11907237381043542No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA597910.11746920821851838No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT592550.11641614846696505No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT549860.10802899906513443No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC546970.10746121125133046No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC544240.10692485805697588No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT531850.10449063971336657No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA524010.10295034336034825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT301450.065.988031
GCGTGTT427450.046.8072432
CGTGTTT613500.033.250533
GTTTTTT5663700.031.8697871
TCGACTC40550.031.8438723
ACTCGTC461400.031.39871242-43
TCGTCTA479100.030.15316244-45
TTCGACT45550.029.4916382
CTAACGA488100.028.51065894-95
CCCGTAT51450.026.4890651
TTAGGCA431300.025.7551528
GTCTATG572200.025.51952246-47
CCCGAAA532050.025.5171281
GCCTAAC558450.025.11530592-93
CCTTTAG447900.025.0097375
GTGGCAA581800.024.97835252-53
CAAAACG329400.024.9612149
TAGGCAT441100.024.9469729
ATGTGGC622700.023.37050850-51
CTATGTG624250.023.31237648-49
Produced by FastQC (version 0.11.5)