##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233038_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50899296 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.143514008523812 31.0 30.0 34.0 23.0 34.0 2 29.82508815053159 31.0 30.0 34.0 25.0 34.0 3 27.59802974485148 31.0 26.0 34.0 10.0 34.0 4 31.019104920429548 35.0 30.0 37.0 17.0 37.0 5 32.187570492134114 35.0 32.0 37.0 19.0 37.0 6 32.73112028897217 35.0 33.0 37.0 26.0 37.0 7 33.119593579447546 36.0 35.0 37.0 27.0 37.0 8 33.16363660511139 37.0 35.0 37.0 27.0 37.0 9 34.61478883715798 39.0 35.0 39.0 27.0 39.0 10-11 34.500201790610234 39.0 35.0 39.0 27.0 39.0 12-13 34.42238456500459 39.0 35.0 39.0 27.0 39.0 14-15 35.46002342547135 40.0 36.0 41.0 26.5 41.0 16-17 35.277032643044805 40.0 35.0 41.0 25.0 41.0 18-19 35.054356576955406 39.5 35.0 41.0 24.5 41.0 20-21 34.843160149012675 39.5 34.5 41.0 21.5 41.0 22-23 34.65168382682542 39.0 34.0 41.0 18.5 41.0 24-25 34.45024940030605 39.0 34.0 41.0 18.0 41.0 26-27 34.153116685543154 39.0 34.0 41.0 13.5 41.0 28-29 33.93503063382252 39.0 34.0 41.0 10.0 41.0 30-31 33.74461607484709 39.0 33.5 41.0 8.5 41.0 32-33 33.56231734521436 39.0 33.0 41.0 7.5 41.0 34-35 33.59342914094529 39.0 33.0 41.0 2.0 41.0 36-37 33.529698063014465 39.0 33.0 41.0 2.0 41.0 38-39 33.45052119188446 39.0 33.0 41.0 2.0 41.0 40-41 33.34560714749375 39.0 33.0 41.0 2.0 41.0 42-43 33.24310702647047 39.0 33.0 41.0 2.0 41.0 44-45 33.113954778470806 39.0 33.0 41.0 2.0 41.0 46-47 32.987153486366495 39.0 33.0 41.0 2.0 41.0 48-49 33.00364453960228 39.0 33.0 41.0 2.0 41.0 50-51 32.979504176246365 39.0 33.0 41.0 2.0 41.0 52-53 32.851231311725805 39.0 33.0 41.0 2.0 41.0 54-55 32.71322744817532 39.0 32.5 41.0 2.0 41.0 56-57 32.57761530729226 38.5 32.0 41.0 2.0 41.0 58-59 32.38121977168407 38.0 32.0 40.0 2.0 41.0 60-61 32.18363574223109 38.0 32.0 40.0 2.0 41.0 62-63 31.920024571656157 38.0 31.0 40.0 2.0 41.0 64-65 31.453473452756597 37.0 31.0 40.0 2.0 41.0 66-67 31.260731199504214 37.0 31.0 40.0 2.0 41.0 68-69 30.849256186175936 36.0 30.0 39.5 2.0 41.0 70-71 30.574420361334663 36.0 30.0 39.0 2.0 41.0 72-73 30.277196859461476 35.5 30.0 39.0 2.0 40.0 74-75 29.89241181253273 35.0 29.0 38.0 2.0 40.0 76-77 28.729236657811533 34.0 28.5 36.5 2.0 39.0 78-79 29.187681918036745 35.0 29.0 37.0 2.0 39.0 80-81 29.007050460972977 35.0 29.0 36.5 2.0 39.0 82-83 28.690968260150395 35.0 29.0 36.0 2.0 38.0 84-85 28.382398795849753 34.0 29.0 36.0 2.0 37.0 86-87 28.024421035214317 34.0 28.5 35.0 2.0 37.0 88-89 27.76662862881247 34.0 28.0 35.0 2.0 36.0 90-91 27.517173046951378 34.0 27.5 35.0 2.0 36.0 92-93 27.266528764955808 34.0 27.0 35.0 2.0 36.0 94-95 27.004565711478605 34.0 26.0 35.0 2.0 35.5 96-97 26.725494739652195 34.0 26.0 35.0 2.0 35.0 98-99 26.40854770368533 34.0 25.0 35.0 2.0 35.0 100-101 25.47404472942023 33.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.375812128803233 1101 2 1.9818922723555445 1101 3 4.172548820950524 1101 4 4.150021686158716 1101 5 2.812543482188154 1101 6 2.2204797501930926 1101 7 1.9097091045620473 1101 8 1.7812648256997434 1101 9 1.9786929834987887 1101 10-11 1.915910530736113 1101 12-13 1.9071875499395858 1101 14-15 2.154541065003855 1101 16-17 2.186046027154589 1101 18-19 2.1337214966718747 1101 20-21 2.1662480685494927 1101 22-23 2.1346403467032147 1101 24-25 2.1425843480029982 1101 26-27 2.077245220135552 1101 28-29 2.109119355768499 1101 30-31 2.0773198551112557 1101 32-33 2.0677245740533294 1101 34-35 2.1151601500741606 1101 36-37 2.1241411073059737 1101 38-39 2.14939011883461 1101 40-41 2.186236680100521 1101 42-43 2.1389922185482106 1101 44-45 2.172721665219484 1101 46-47 2.129515398613222 1101 48-49 2.253569372283309 1101 50-51 2.1842995360603155 1101 52-53 2.1147579176472675 1101 54-55 2.083855084466016 1101 56-57 2.10621167541715 1101 58-59 2.1329557481974817 1101 60-61 2.113837128939373 1101 62-63 2.1113096443060897 1101 64-65 1.680770056447038 1101 66-67 2.0397201770848845 1101 68-69 1.9133330444291765 1101 70-71 1.9935092600512796 1101 72-73 2.022607835411911 1101 74-75 2.1264535979022305 1101 76-77 1.9373488856436687 1101 78-79 2.1174641631036373 1101 80-81 2.079749144030526 1101 82-83 2.0750397420662523 1101 84-85 2.0953779289779675 1101 86-87 2.0755109747639793 1101 88-89 2.114745429261223 1101 90-91 2.1845526416987795 1101 92-93 2.148468860871276 1101 94-95 2.161159142270897 1101 96-97 2.269320952602005 1101 98-99 2.2090745519099997 1101 100-101 2.290368669785309 1102 1 2.324175190873799 1102 2 1.9690442761616787 1102 3 4.17250590965352 1102 4 4.164204618648434 1102 5 2.7616642042527317 1102 6 2.1472578396678657 1102 7 1.8179565292789803 1102 8 1.6657011833871422 1102 9 1.835803433857599 1102 10-11 1.8859320096332368 1102 12-13 1.9093097167406512 1102 14-15 2.1104688771999562 1102 16-17 2.0621481951302414 1102 18-19 2.0654334381363313 1102 20-21 2.054266978657438 1102 22-23 2.09604064127533 1102 24-25 2.123192658442342 1102 26-27 2.0369399305739435 1102 28-29 2.0872080642457718 1102 30-31 2.064772992677817 1102 32-33 2.0628852977914818 1102 34-35 2.1193585534447053 1102 36-37 2.0892305123439385 1102 38-39 2.118643697394596 1102 40-41 2.15517534125361 1102 42-43 2.1586727636147174 1102 44-45 2.1754870414773535 1102 46-47 2.184037559173852 1102 48-49 2.189933314703204 1102 50-51 2.1965204241997753 1102 52-53 2.2380123966351704 1102 54-55 2.249225566921858 1102 56-57 2.2448873871888324 1102 58-59 2.257333951754177 1102 60-61 2.215171838517307 1102 62-63 2.217838356526144 1102 64-65 2.161388967328101 1102 66-67 2.180830992417736 1102 68-69 1.9876538960453196 1102 70-71 2.083303982799311 1102 72-73 2.1169745733372167 1102 74-75 2.171860619594373 1102 76-77 2.0868242535000014 1102 78-79 2.1685010983923725 1102 80-81 2.129660935255579 1102 82-83 2.1164245537277715 1102 84-85 2.112943218712193 1102 86-87 2.07394148240364 1102 88-89 2.062508668426908 1102 90-91 2.075179552018721 1102 92-93 2.132786586279824 1102 94-95 2.1518730717237347 1102 96-97 2.2092217667510425 1102 98-99 2.1201222429489164 1102 100-101 2.178567019136466 1103 1 2.2007839617386615 1103 2 1.8297712439492102 1103 3 4.043350452609648 1103 4 4.058723730580311 1103 5 2.6776813302764424 1103 6 2.076761246253234 1103 7 1.6994143525982253 1103 8 1.6492124101093637 1103 9 1.8120500769080579 1103 10-11 1.809250055605986 1103 12-13 1.8130902748373643 1103 14-15 1.9502560261748911 1103 16-17 1.9960225233840987 1103 18-19 2.0031960449246142 1103 20-21 1.9798812457998451 1103 22-23 1.993867007543102 1103 24-25 1.9612717945794103 1103 26-27 1.9704888947762953 1103 28-29 1.9986314120376178 1103 30-31 1.9596774057068131 1103 32-33 1.9816149387890576 1103 34-35 1.9888273942557078 1103 36-37 2.0201943166619216 1103 38-39 2.042884934176982 1103 40-41 2.062922787739531 1103 42-43 2.039715510276295 1103 44-45 2.037533217998515 1103 46-47 1.9912039339628436 1103 48-49 2.039232018614314 1103 50-51 1.996393003799973 1103 52-53 2.0252743583951514 1103 54-55 2.10369660928378 1103 56-57 2.0750103629257026 1103 58-59 2.0504373263256213 1103 60-61 2.144209345155211 1103 62-63 2.162073600197701 1103 64-65 2.04486039632701 1103 66-67 2.100603015526655 1103 68-69 1.9765019838071645 1103 70-71 1.9760016497728223 1103 72-73 1.9764141860067497 1103 74-75 2.0145489709438458 1103 76-77 1.9084803719591115 1103 78-79 2.0205433693574584 1103 80-81 2.0228389001295604 1103 82-83 2.06288554281549 1103 84-85 2.0428870839136266 1103 86-87 1.9928221683185612 1103 88-89 1.8923695420460085 1103 90-91 1.8783759213358913 1103 92-93 1.8259738964643368 1103 94-95 1.8831963439456239 1103 96-97 1.9121889978412163 1103 98-99 1.884870912281194 1103 100-101 1.9770497658170392 1104 1 2.1814730707007755 1104 2 1.7925605443131936 1104 3 3.9857159141588347 1104 4 4.020295596514188 1104 5 2.6309822261010396 1104 6 2.0767781627643984 1104 7 1.695242524763266 1104 8 1.64184917446849 1104 9 1.7377269896350782 1104 10-11 1.7249506744934067 1104 12-13 1.6990919531255315 1104 14-15 1.873385538593503 1104 16-17 1.8914719687761163 1104 18-19 1.8136386091203818 1104 20-21 1.8388936685301616 1104 22-23 1.8162839072319272 1104 24-25 1.8065523536122754 1104 26-27 1.8304320491983646 1104 28-29 1.8407356577656486 1104 30-31 1.8651031252222197 1104 32-33 1.8236625699899847 1104 34-35 1.7927590239850346 1104 36-37 1.8351509931764554 1104 38-39 1.845560075136632 1104 40-41 1.8953381823042221 1104 42-43 1.8318284877933735 1104 44-45 1.905508770547911 1104 46-47 1.8947838001168194 1104 48-49 1.9218555806141708 1104 50-51 1.902734018951648 1104 52-53 1.8569273484406352 1104 54-55 1.9326664557905175 1104 56-57 1.9311063766962135 1104 58-59 1.9118505999093642 1104 60-61 1.9455142922024038 1104 62-63 1.9675985313513706 1104 64-65 1.85210088218615 1104 66-67 1.9592596151132788 1104 68-69 1.8417734141868074 1104 70-71 1.8736265781004668 1104 72-73 1.916796440613446 1104 74-75 1.9243189313620483 1104 76-77 1.8645163554265203 1104 78-79 1.9119267487428182 1104 80-81 1.8277568432960827 1104 82-83 1.8405506031941243 1104 84-85 1.9592426348204839 1104 86-87 1.9311450534098924 1104 88-89 1.856232165816813 1104 90-91 1.9418556380325072 1104 92-93 1.9187287514320026 1104 94-95 1.950961260184009 1104 96-97 1.8868561152947478 1104 98-99 2.00553874306501 1104 100-101 2.049773518504338 1105 1 1.8656519571810897 1105 2 1.5422386023389265 1105 3 3.1004641708320015 1105 4 3.5254234655402072 1105 5 2.1663427475784047 1105 6 1.6622140227606295 1105 7 1.403730480278476 1105 8 1.3240618902627332 1105 9 1.3821695584699754 1105 10-11 1.4168391089852221 1105 12-13 1.3965890791965379 1105 14-15 1.5324169642849057 1105 16-17 1.5517465835591082 1105 18-19 1.5275289713778761 1105 20-21 1.5346054860786893 1105 22-23 1.50519666694845 1105 24-25 1.6361477465375955 1105 26-27 1.6119354579391043 1105 28-29 1.6068706629875251 1105 30-31 1.5561887559558087 1105 32-33 1.6000704694598227 1105 34-35 1.5436926159138622 1105 36-37 1.5081586823756439 1105 38-39 1.5602124203424808 1105 40-41 1.595286718279489 1105 42-43 1.5511228377110484 1105 44-45 1.627344707054263 1105 46-47 1.6795802612005701 1105 48-49 1.6489373384250001 1105 50-51 1.6578120441758273 1105 52-53 1.6780480222309535 1105 54-55 1.6386720439388895 1105 56-57 1.6952512164486393 1105 58-59 1.6601686166969216 1105 60-61 1.651924334720249 1105 62-63 1.6788847404741674 1105 64-65 1.5143204392462977 1105 66-67 1.5965689110216736 1105 68-69 1.537846669482878 1105 70-71 1.5477225149374085 1105 72-73 1.6230135937123755 1105 74-75 1.5935350790049903 1105 76-77 1.5278301667962673 1105 78-79 1.632032270172811 1105 80-81 1.662378618220476 1105 82-83 1.6540590515991305 1105 84-85 1.6739139590170602 1105 86-87 1.666577047400601 1105 88-89 1.6250806393685835 1105 90-91 1.6209582936109754 1105 92-93 1.7347791230871792 1105 94-95 1.76431040871352 1105 96-97 1.670547267329411 1105 98-99 1.7231142024880128 1105 100-101 1.79644719630722 1106 1 1.8568899244288346 1106 2 1.507554241677294 1106 3 3.707845514773517 1106 4 3.7599688292493596 1106 5 2.280342213490279 1106 6 1.6912389746514975 1106 7 1.343196094854548 1106 8 1.247102372640768 1106 9 1.3040511816025955 1106 10-11 1.3117490606983395 1106 12-13 1.3187762837469563 1106 14-15 1.4457933129679361 1106 16-17 1.4309670530535286 1106 18-19 1.291688775733867 1106 20-21 1.338495506124552 1106 22-23 1.2486018222469895 1106 24-25 1.295658074895293 1106 26-27 1.3447142612115783 1106 28-29 1.4196529181250455 1106 30-31 1.312214809112021 1106 32-33 1.3644925053066146 1106 34-35 1.347739648276658 1106 36-37 1.302705082131503 1106 38-39 1.3336886218834536 1106 40-41 1.3495224670392432 1106 42-43 1.4783252473653832 1106 44-45 1.4149585518513916 1106 46-47 1.3460563349014691 1106 48-49 1.3846303257963513 1106 50-51 1.2981458390803553 1106 52-53 1.525521000052258 1106 54-55 1.6231984182784984 1106 56-57 1.5611638194461435 1106 58-59 1.5225997358701662 1106 60-61 1.563790266616138 1106 62-63 1.5523365048426143 1106 64-65 1.4856276185352186 1106 66-67 1.5551590716300971 1106 68-69 1.5068624657893999 1106 70-71 1.4740126839054675 1106 72-73 1.4273500477904975 1106 74-75 1.4280547430013222 1106 76-77 1.3471107606156814 1106 78-79 1.4978244533084073 1106 80-81 1.4807749701554727 1106 82-83 1.5043274044480839 1106 84-85 1.5264474180298748 1106 86-87 1.5578112868487857 1106 88-89 1.5256282059181387 1106 90-91 1.5371358053493616 1106 92-93 1.5710190325150357 1106 94-95 1.5105703576290708 1106 96-97 1.5113161650820572 1106 98-99 1.5515282934428036 1106 100-101 1.513840991978924 1107 1 1.6295795043184285 1107 2 1.3392006868294537 1107 3 2.2657950361276917 1107 4 3.0639437297011014 1107 5 1.953833196391713 1107 6 1.4795620688155111 1107 7 1.1752489115515132 1107 8 1.1040264810118074 1107 9 1.1970714551864177 1107 10-11 1.0626437997492602 1107 12-13 1.1205592245196527 1107 14-15 1.172093537403157 1107 16-17 1.2237050073860445 1107 18-19 1.2415717358280958 1107 20-21 1.244276683650149 1107 22-23 1.2370325514507172 1107 24-25 1.261695285654156 1107 26-27 1.2640771342679002 1107 28-29 1.2212431727355835 1107 30-31 1.203538112633396 1107 32-33 1.2742463665011314 1107 34-35 1.275300510459104 1107 36-37 1.268151259420982 1107 38-39 1.2803802311904349 1107 40-41 1.297572095240561 1107 42-43 1.2863220538637279 1107 44-45 1.3242921974757138 1107 46-47 1.2752085971861291 1107 48-49 1.3416384594220858 1107 50-51 1.3314977032479902 1107 52-53 1.2591341384903671 1107 54-55 1.123615032326363 1107 56-57 1.1681966110319806 1107 58-59 1.2597704279398343 1107 60-61 1.3275795689496732 1107 62-63 1.3339272785063798 1107 64-65 1.2340934434822657 1107 66-67 1.358160770187279 1107 68-69 1.2480257749162114 1107 70-71 1.3151184887032912 1107 72-73 1.3631308566587705 1107 74-75 1.30781655431376 1107 76-77 1.1957988296828326 1107 78-79 1.27060255725549 1107 80-81 1.337892820411863 1107 82-83 1.3718267145921175 1107 84-85 1.349077358354105 1107 86-87 1.3501793791929835 1107 88-89 1.3146693327044332 1107 90-91 1.2467457709675998 1107 92-93 1.2903843279051301 1107 94-95 1.2587206908375492 1107 96-97 1.2103024852844655 1107 98-99 1.306241411755515 1107 100-101 1.317217933241917 1108 1 1.4307350451119305 1108 2 1.1583502607441538 1108 3 1.4078100437695689 1108 4 2.6657656331149653 1108 5 1.618072617357484 1108 6 1.2206177183396036 1108 7 0.996018751664252 1108 8 0.9033775585005941 1108 9 0.8781949473002371 1108 10-11 0.9452623035532142 1108 12-13 0.930620099754222 1108 14-15 0.9580147354019957 1108 16-17 1.0085114066395349 1108 18-19 0.9843327532563677 1108 20-21 0.969920436902342 1108 22-23 0.9808815225813206 1108 24-25 1.0062013048223974 1108 26-27 0.9874078720494879 1108 28-29 1.0255920916876846 1108 30-31 1.0195476885021932 1108 32-33 0.9853666679232305 1108 34-35 0.9948390925722421 1108 36-37 0.9750124443969952 1108 38-39 0.9905141129985964 1108 40-41 0.9653797452004227 1108 42-43 0.9938068092635888 1108 44-45 1.0366737757173041 1108 46-47 0.9896292017171078 1108 48-49 0.9963619963830723 1108 50-51 0.9473560418793028 1108 52-53 0.9527866602823707 1108 54-55 0.959952309625983 1108 56-57 0.8901891311030923 1108 58-59 0.9542154179828017 1108 60-61 0.8568982297521046 1108 62-63 0.9339396535722386 1108 64-65 0.895053039054801 1108 66-67 0.9645655913468509 1108 68-69 0.8620056156405518 1108 70-71 0.8001402281060805 1108 72-73 0.8053280041946174 1108 74-75 0.8473618834600174 1108 76-77 0.7513611812869812 1108 78-79 0.8700986055690301 1108 80-81 0.9355871321295552 1108 82-83 0.9256891272816787 1108 84-85 0.9429701584248065 1108 86-87 0.9518161466672339 1108 88-89 0.9327095060311095 1108 90-91 0.8966454762112974 1108 92-93 0.9621212633866385 1108 94-95 0.9212375884108575 1108 96-97 0.878661414872159 1108 98-99 0.835624755638154 1108 100-101 0.8377294726197064 1109 1 1.0138628460260009 1109 2 0.7952344885380036 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2.191636838561717 2204 68-69 2.2285028475214794 2204 70-71 2.1794552462678034 2204 72-73 2.191403041873407 2204 74-75 2.1683997283095557 2204 76-77 2.178716985448741 2204 78-79 2.099836941030599 2204 80-81 2.0299300757672505 2204 82-83 2.0427254595015043 2204 84-85 2.044089683503209 2204 86-87 2.119318757567534 2204 88-89 2.0600195570670365 2204 90-91 2.11701599248196 2204 92-93 2.1565532716712497 2204 94-95 2.2115615077067297 2204 96-97 2.2610742819242375 2204 98-99 2.3258748100398883 2204 100-101 2.3579934459294662 2205 1 2.0825400056125716 2205 2 1.7172408593476547 2205 3 3.925742285068843 2205 4 3.8963014339107787 2205 5 2.3973880558983183 2205 6 1.790988905051627 2205 7 1.432904613978117 2205 8 1.3141949726592657 2205 9 1.3981691127806926 2205 10-11 1.4217814438248908 2205 12-13 1.4944212880578078 2205 14-15 1.684559025251687 2205 16-17 1.7055754006506731 2205 18-19 1.6905354181978325 2205 20-21 1.6787241515054774 2205 22-23 1.6319775819304496 2205 24-25 1.679595268172129 2205 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18-19 -4.667132418036324 2316 20-21 -4.700699978140719 2316 22-23 -4.74531770766929 2316 24-25 -4.7647981274694615 2316 26-27 -4.840532780990195 2316 28-29 -4.787281968972124 2316 30-31 -4.910766803159767 2316 32-33 -4.859221182848515 2316 34-35 -4.891782987544097 2316 36-37 -4.966294042805281 2316 38-39 -5.013649274419471 2316 40-41 -4.956814560692205 2316 42-43 -4.891687951862544 2316 44-45 -4.884426898567256 2316 46-47 -5.020514406191317 2316 48-49 -4.9678974535737055 2316 50-51 -4.984917708885071 2316 52-53 -5.0221697221688935 2316 54-55 -5.001815041994785 2316 56-57 -5.073212477742292 2316 58-59 -5.024620903575915 2316 60-61 -5.078062418729115 2316 62-63 -5.088897511731616 2316 64-65 -4.950770427067347 2316 66-67 -5.080389109861397 2316 68-69 -4.978996735232013 2316 70-71 -4.94677681213593 2316 72-73 -4.910911781596635 2316 74-75 -4.876111171247604 2316 76-77 -4.720858389382588 2316 78-79 -4.916113930079529 2316 80-81 -4.8892490349194055 2316 82-83 -4.993882162474918 2316 84-85 -4.922201068488498 2316 86-87 -4.849743898410601 2316 88-89 -4.799305025997548 2316 90-91 -4.821673260475475 2316 92-93 -4.839642818625467 2316 94-95 -4.863758908416621 2316 96-97 -4.796558016662146 2316 98-99 -4.790912498105801 2316 100-101 -4.677030715147843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2007150.0 3 480665.0 4 291679.0 5 283740.0 6 409205.0 7 358662.0 8 315328.0 9 388644.0 10 469392.0 11 404370.0 12 316878.0 13 266947.0 14 249672.0 15 247138.0 16 249721.0 17 250173.0 18 249769.0 19 249023.0 20 251747.0 21 265553.0 22 291896.0 23 335672.0 24 388940.0 25 454504.0 26 532148.0 27 624794.0 28 736935.0 29 864466.0 30 1020233.0 31 1242718.0 32 1466353.0 33 1830886.0 34 2382866.0 35 3258884.0 36 4757431.0 37 8199414.0 38 1.1404267E7 39 3095262.0 40 6171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.211674003546104 16.017116581221742 14.084390938372099 43.686818476860054 2 15.640654204046701 19.496464152203057 51.0148186729858 13.848062970764444 3 21.34685020424705 22.89335111968277 34.935602307414484 20.8241963686557 4 22.58846982394452 30.463842255044277 29.888418031363194 17.059269889648007 5 21.13444053502603 33.09301788929375 30.982089167048617 14.79045240863161 6 15.852276481331163 36.457559217575024 31.95346069415835 15.736703606935464 7 13.366277532650495 19.55851433519475 49.801324328717556 17.273883803437197 8 14.192463082549677 21.574097892318704 42.719486399439845 21.513952625691772 9 16.14217468028436 22.71187851435336 40.98585482611625 20.16009197924603 10-11 19.217730544482478 33.77658133453765 30.42093205064951 16.584756070330364 12-13 17.98870106839213 26.677233896212332 37.12501896909078 18.209046066304754 14-15 18.933826344063824 28.02410546861362 35.61653651781155 17.42553166951101 16-17 19.80875794257662 27.82557084513802 33.97104207266607 18.394629139619283 18-19 19.74923661256937 28.361406313739863 33.31528500210738 18.574072071583384 20-21 20.133420050497403 27.8301247287169 32.70035190089191 19.336103319893787 22-23 19.60847831002868 29.475494556344195 33.157384513922224 17.758642619704897 24-25 19.40040608567245 29.308200424183077 32.19597575529912 19.09541773484535 26-27 19.514501329091644 29.737010580862762 31.922091742784502 18.826396347261095 28-29 19.861088124978725 29.392719935920926 31.61207630122933 19.134115637871023 30-31 19.053780680406266 29.684912156674457 31.74504084113344 19.51626632178583 32-33 19.252393106288544 29.37873296091974 31.928725809870922 19.440148122920796 34-35 19.590995404346973 30.400931685840305 30.59448663764133 19.413586272171393 36-37 19.981738907436387 30.164962682842333 30.979968237119383 18.8733301726019 38-39 19.65872618293659 29.608304081555776 31.338225917666684 19.394743817840958 40-41 19.86669509534256 29.921825604605413 30.743188021723917 19.468291278328113 42-43 19.64340414563044 30.112956220353524 31.01264534276452 19.23099429125151 44-45 19.89337639263478 29.71649551995286 30.997733009550892 19.39239507786146 46-47 19.638799284444904 29.8057343402316 31.124231277531457 19.43123509779204 48-49 19.669820527780516 29.37485836395271 30.97903699854538 19.976284109721394 50-51 19.22018522738702 29.86568378125345 31.649396707111343 19.26473428424819 52-53 20.34432853401425 29.53190520483908 30.67663136472463 19.447134896422043 54-55 19.743517782133768 29.881958363254068 30.54201781191009 19.83250604270208 56-57 20.588090349140675 29.672556485099516 30.09333896429944 19.646014201460364 58-59 19.511745197622567 30.44526357566791 30.32086789299622 19.722123333713306 60-61 19.888832832883306 30.2099418318549 29.72528902626385 20.175936308997947 62-63 19.779635307375028 30.56306486967051 30.10423355730541 19.553066265649054 64-65 20.098041783339447 30.173600458553963 29.977858595739754 19.750499162366836 66-67 20.038885172408385 30.535341829590717 29.582461561794627 19.843311436206267 68-69 20.254393887321072 30.412126715366384 30.00181873318849 19.331660664124055 70-71 19.926139471433995 30.69599347248707 29.636084158763314 19.74178289731562 72-73 19.927903275775403 30.53520720975887 29.8273918296424 19.709497684823326 74-75 19.814919564213927 29.998226587523742 30.941447390896986 19.24540645736535 76-77 19.649318477399877 30.418807937695313 30.343745650953956 19.588127933950847 78-79 19.823305440897627 30.707553044484833 29.883023041334116 19.586118473283427 80-81 19.93757438323595 30.520675085850357 30.064949846917084 19.476800683996608 82-83 20.1848596207047 30.669249424827104 29.640375324931505 19.50551562953669 84-85 19.790083732967595 30.441069785700453 29.8372979976154 19.931548483716554 86-87 19.7320406208003 31.21132948611808 29.610445752874693 19.446184140206928 88-89 19.589920720678762 30.66476153781867 30.191522540200623 19.55379520130195 90-91 19.678703161182256 31.29049270036367 29.575043754139717 19.455760384314353 92-93 20.073899272237426 30.591183664066897 29.560496941108518 19.77442012258716 94-95 19.900938673130767 30.917917612283784 29.41274431696488 19.768399397620566 96-97 19.882448684418357 30.889912568040234 29.403609046271168 19.82402970127025 98-99 20.16015765828679 30.799471596826127 29.181531882849608 19.858838862037473 100-101 20.860543205100647 31.510593102487245 28.295368753466434 19.333494938945677 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23921.0 1 24270.5 2 21020.0 3 18313.5 4 20970.5 5 24793.5 6 27489.0 7 29788.0 8 33034.0 9 36806.5 10 41098.0 11 46485.5 12 53142.0 13 60063.0 14 68483.5 15 78082.0 16 88357.0 17 100283.5 18 114354.0 19 130435.5 20 152372.5 21 182569.5 22 217780.0 23 261503.5 24 324732.0 25 416730.5 26 559193.0 27 765754.0 28 916002.5 29 1024348.0 30 1209800.0 31 1413743.0 32 1608737.0 33 1816494.5 34 2079458.5 35 2361603.5 36 2599919.0 37 2888412.5 38 2982269.0 39 2518276.0 40 2242302.5 41 2204484.5 42 1900305.5 43 1680685.5 44 1576359.0 45 1472735.0 46 1354075.0 47 1272306.0 48 1229412.5 49 1192096.5 50 1099482.5 51 960434.0 52 850106.0 53 763088.0 54 690147.0 55 620612.0 56 550175.0 57 469102.5 58 384894.0 59 297930.5 60 211909.0 61 148366.0 62 104400.0 63 73769.5 64 50616.0 65 34396.0 66 24861.5 67 18460.5 68 13335.0 69 9603.5 70 8188.5 71 8263.0 72 7760.5 73 8120.5 74 8033.0 75 5560.0 76 4203.0 77 4014.0 78 3555.5 79 2533.0 80 1878.0 81 1585.0 82 1290.0 83 1072.0 84 1050.0 85 935.0 86 706.0 87 482.0 88 274.0 89 160.0 90 100.5 91 66.0 92 40.0 93 22.0 94 10.5 95 5.5 96 4.0 97 2.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043379774840107806 2 0.003980408687774385 3 0.030118294759911807 4 0.007811502933164341 5 0.030141870724498823 6 0.11862050115585096 7 0.14093318697374518 8 0.13888404271839044 9 0.15547562779650234 10-11 0.15710531634857977 12-13 0.16321542050404783 14-15 0.17387666815666764 16-17 0.1859043394234765 18-19 0.1811380652494683 20-21 0.1837235626991776 22-23 0.15600215767227898 24-25 0.16775870534633722 26-27 0.1767617768230036 28-29 0.1821174501116872 30-31 0.19325905804276744 32-33 0.19461074667908965 34-35 0.19601253424015924 36-37 0.18792892538238642 38-39 0.20590559837998546 40-41 0.2257261082746606 42-43 0.21518961676798043 44-45 0.24371456925455312 46-47 0.23351894690252692 48-49 0.2313037885632053 50-51 0.23222030418652548 52-53 0.23359458645557693 54-55 0.23711133450647334 56-57 0.23453664270719973 58-59 0.2432646612636843 60-61 0.2488462708796601 62-63 0.2523777539084234 64-65 0.416767060982533 66-67 0.21563461309956036 68-69 0.375100472902415 70-71 0.01619668767127938 72-73 0.003065857728169757 74-75 0.022651590308832563 76-77 0.037102674268815035 78-79 0.018220291298331512 80-81 0.024546508462513904 82-83 0.04404776050340657 84-85 0.02508482632058408 86-87 0.016451111622447587 88-89 0.030645806967546266 90-91 0.008639608689283247 92-93 0.02740804116426286 94-95 0.02899156011902404 96-97 0.024722345865058722 98-99 0.020633880672927186 100-101 0.011491318072454284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.0899296E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.63511971307249 #Duplication Level Percentage of deduplicated Percentage of total 1 75.35672029228446 20.824919864618003 2 9.211891079904461 5.091434255538888 3 4.103396811802951 3.4019358637324366 4 2.335449112846563 2.5816166326921484 5 1.5523092123465567 2.144912545745118 6 1.0806074588511012 1.7917629892913547 7 0.8369398436458845 1.6190252940255934 8 0.6536256671296621 1.4450418846932065 9 0.5262353939890533 1.308832029912902 >10 3.7345552372255844 20.17877882365075 >50 0.3677266168609365 7.012472847643782 >100 0.20709113463277976 10.562743534124907 >500 0.016773777105385263 3.198104437867845 >1k 0.013647587860766055 7.8144788168380455 >5k 0.0018109808813834927 3.429822438278697 >10k+ 0.0012197926324756263 7.594117741346558 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 127075 0.24965964165791213 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 121502 0.2387105707709592 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 87498 0.1719041457862207 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 80607 0.15836564812212725 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 75794 0.14890972165901864 No Hit CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA 60607 0.11907237381043542 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA 59791 0.11746920821851838 No Hit GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT 59255 0.11641614846696505 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 54986 0.10802899906513443 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 54697 0.10746121125133046 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 54424 0.10692485805697588 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 53185 0.10449063971336657 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 52401 0.10295034336034825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.028348132752170088 0.0 0.0 1.7681973440261336E-5 0.0 2 0.028599609707764918 5.8939911467537785E-6 0.0 1.7681973440261336E-5 0.0 3 0.02879804074303896 5.8939911467537785E-6 0.0 1.7681973440261336E-5 0.0 4 0.030917912892154738 5.8939911467537785E-6 0.0 1.9646637155845928E-5 0.0 5 0.03136978554673919 5.8939911467537785E-6 0.0 2.75052920181843E-5 1.9646637155845927E-6 6 0.03182165820132365 5.8939911467537785E-6 0.0 3.143461944935348E-5 1.9646637155845927E-6 7 0.0323501527408159 5.8939911467537785E-6 0.0 3.7328610596107264E-5 3.929327431169185E-6 8 0.03898285744462949 5.8939911467537785E-6 0.0 3.7328610596107264E-5 3.929327431169185E-6 9 0.039391507497471084 5.8939911467537785E-6 0.0 3.9293274311691856E-5 3.929327431169185E-6 10-11 0.04137778251392711 5.8939911467537785E-6 0.0 4.322260174286104E-5 4.911659288961482E-6 12-13 0.0439544389769163 5.8939911467537785E-6 0.0 4.911659288961482E-5 6.876323004546075E-6 14-15 0.046886699572426305 5.8939911467537785E-6 1.9646637155845927E-6 5.108125660519941E-5 7.85865486233837E-6 16-17 0.04951149029644732 5.8939911467537785E-6 1.9646637155845927E-6 5.40282521785763E-5 7.85865486233837E-6 18-19 0.05251546111757617 5.8939911467537785E-6 1.9646637155845927E-6 6.385157075649927E-5 7.85865486233837E-6 20-21 0.056393707292140154 8.840986720130668E-6 1.9646637155845927E-6 7.563955305000682E-5 7.85865486233837E-6 22-23 0.06033482270560285 9.823318577922964E-6 1.9646637155845927E-6 7.760421676559142E-5 7.85865486233837E-6 24-25 0.06290853217301866 9.823318577922964E-6 1.9646637155845927E-6 8.349820791234518E-5 7.85865486233837E-6 26-27 0.0652238883618351 9.823318577922964E-6 1.9646637155845927E-6 9.430385834806046E-5 7.85865486233837E-6 28-29 0.06777205720094831 9.823318577922964E-6 1.9646637155845927E-6 9.823318577922964E-5 7.85865486233837E-6 30-31 0.07047248747801935 1.375264600909215E-5 1.9646637155845927E-6 1.06091840641568E-4 7.85865486233837E-6 32-33 0.07300592133926567 1.375264600909215E-5 1.9646637155845927E-6 1.0707417249936031E-4 7.85865486233837E-6 34-35 0.0757839558331023 1.375264600909215E-5 1.9646637155845927E-6 1.090388362149449E-4 8.840986720130668E-6 36-37 0.0786916581321675 1.375264600909215E-5 1.9646637155845927E-6 1.1787982293507557E-4 9.823318577922964E-6 38-39 0.08174671020990153 1.4734977866884446E-5 1.9646637155845927E-6 1.2180915036624475E-4 9.823318577922964E-6 40-41 0.08486463152653428 1.571730972467674E-5 1.9646637155845927E-6 1.2475614593962164E-4 9.823318577922964E-6 42-43 0.08838432657300407 1.7681973440261336E-5 1.9646637155845927E-6 1.335971326597523E-4 9.823318577922964E-6 44-45 0.09226453741128365 1.7681973440261336E-5 1.9646637155845927E-6 1.4734977866884448E-4 9.823318577922964E-6 46-47 0.09669878341735808 1.7681973440261336E-5 1.9646637155845927E-6 1.7092574325585958E-4 1.669964158246904E-5 48-49 0.10117232269774418 1.7681973440261336E-5 1.9646637155845927E-6 1.8271372554936715E-4 1.7681973440261336E-5 50-51 0.10655550127844597 1.7681973440261336E-5 1.9646637155845927E-6 1.895900485539132E-4 1.7681973440261336E-5 52-53 0.11256737224813482 1.7681973440261336E-5 1.9646637155845927E-6 1.9646637155845925E-4 1.7681973440261336E-5 54-55 0.11932286843417245 1.7681973440261336E-5 2.946995573376889E-6 2.0432502642079764E-4 1.7681973440261336E-5 56-57 0.12642414543415295 1.7681973440261336E-5 5.8939911467537785E-6 2.1709534057209748E-4 1.7681973440261336E-5 58-59 0.13441541509729327 1.8664305298053632E-5 5.8939911467537785E-6 2.3674197772794342E-4 1.7681973440261336E-5 60-61 0.14342830989253763 2.0628969013638224E-5 5.8939911467537785E-6 2.426359688746972E-4 1.7681973440261336E-5 62-63 0.15385674489486062 2.161130087143052E-5 5.8939911467537785E-6 2.544239511682048E-4 1.7681973440261336E-5 64-65 0.16539128556905777 2.3575964587015114E-5 5.8939911467537785E-6 2.711235927506738E-4 1.7681973440261336E-5 66-67 0.17929422835239214 2.75052920181843E-5 5.8939911467537785E-6 2.888055661909351E-4 1.7681973440261336E-5 68-69 0.1950744073159676 3.143461944935348E-5 5.8939911467537785E-6 2.927348936221043E-4 2.0628969013638224E-5 70-71 0.21338409081335818 3.3399283164938075E-5 5.8939911467537785E-6 2.966642210532735E-4 2.161130087143052E-5 72-73 0.23336275613713792 3.3399283164938075E-5 5.8939911467537785E-6 2.986288847688581E-4 2.161130087143052E-5 74-75 0.25682280556493353 3.438161502273037E-5 5.8939911467537785E-6 3.0550520777340414E-4 2.161130087143052E-5 76-77 0.28485266279517896 3.536394688052267E-5 5.8939911467537785E-6 3.565864643786036E-4 2.161130087143052E-5 78-79 0.31660064610716815 3.536394688052267E-5 5.8939911467537785E-6 3.723037741032803E-4 2.161130087143052E-5 80-81 0.35223080492115255 3.634627873831497E-5 5.8939911467537785E-6 3.8900341568574935E-4 2.161130087143052E-5 82-83 0.39511155517750185 3.9293274311691856E-5 5.8939911467537785E-6 4.017737298370492E-4 2.161130087143052E-5 84-85 0.44641383645070454 3.9293274311691856E-5 5.8939911467537785E-6 4.0865005284159534E-4 2.3575964587015114E-5 86-87 0.5088321850266848 3.9293274311691856E-5 5.8939911467537785E-6 4.194557032773105E-4 2.3575964587015114E-5 88-89 0.581325329136183 3.9293274311691856E-5 5.8939911467537785E-6 4.2633202628185665E-4 2.3575964587015114E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 30145 0.0 65.98803 1 GCGTGTT 42745 0.0 46.807243 2 CGTGTTT 61350 0.0 33.25053 3 GTTTTTT 566370 0.0 31.869787 1 TCGACTC 4055 0.0 31.843872 3 ACTCGTC 46140 0.0 31.398712 42-43 TCGTCTA 47910 0.0 30.153162 44-45 TTCGACT 4555 0.0 29.491638 2 CTAACGA 48810 0.0 28.510658 94-95 CCCGTAT 5145 0.0 26.489065 1 TTAGGCA 43130 0.0 25.755152 8 GTCTATG 57220 0.0 25.519522 46-47 CCCGAAA 53205 0.0 25.517128 1 GCCTAAC 55845 0.0 25.115305 92-93 CCTTTAG 44790 0.0 25.009737 5 GTGGCAA 58180 0.0 24.978352 52-53 CAAAACG 32940 0.0 24.961214 9 TAGGCAT 44110 0.0 24.946972 9 ATGTGGC 62270 0.0 23.370508 50-51 CTATGTG 62425 0.0 23.312376 48-49 >>END_MODULE