FastQCFastQC Report
Fri 27 May 2016
SRR1233037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64827754
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT2986340.4606576374680511No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA2888460.44555916590909506No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA2820910.43513924607044074No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC2401570.3704539879632418No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA1829720.2822433120234275No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1394750.21514704951832825No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG1269270.1957911421703735No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1075830.1659520704666091No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT1071630.16530419980306585No Hit
CTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAAC1030790.15900442887470698No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1021320.15754363478333677No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT946490.14600073912787415No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA846870.13063386400830732No Hit
CAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCT836690.12906354892381433No Hit
CCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCC795190.12266196974832723No Hit
CTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATT755840.11659203865060634No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC753830.11628198626162493No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT743530.11469316058674499No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA717790.11072263894874408No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT694830.10718094598804087No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC666580.10282324450111291No Hit
GCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAA659220.10168792829071327No Hit
CACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGC649320.10016080458378983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT451300.072.694251
GCGTGTT628750.051.908682
CGTGTTT860900.038.579363
CCTTTAG873700.036.966625
TTAGGCA940350.034.2125138
TAGGCAT950600.033.7432679
GTTTTTT7111150.033.2594871
ACTCGTC932450.033.038342-43
TCCTTTA1008200.032.333034
TCGTCTA953000.032.25557344-45
CTAACGA989150.029.95911894-95
CTTTAGG1091050.029.7697146
GTCTATG1041800.029.63398746-47
CTTTCCT1203650.028.3144171
ATGTGGC1090400.028.31420550-51
CTATGTG1132950.027.39391748-49
TTTAGGC1229400.027.3072367
GTGGCAA1138200.027.24226252-53
GCCTAAC1102600.027.15759592-93
CCCGAAA1167350.026.9962771
Produced by FastQC (version 0.11.5)