##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233037_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64827754 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.949468031855616 31.0 30.0 34.0 25.0 34.0 2 30.150100696069156 31.0 31.0 34.0 25.0 34.0 3 30.071707466527375 31.0 30.0 34.0 25.0 34.0 4 33.435470385106974 37.0 35.0 37.0 26.0 37.0 5 33.41459264499584 37.0 35.0 37.0 26.0 37.0 6 33.44489318571796 37.0 35.0 37.0 26.0 37.0 7 33.59713595815767 37.0 35.0 37.0 28.0 37.0 8 33.55050781182393 37.0 35.0 37.0 27.0 37.0 9 34.96647088837907 39.0 35.0 39.0 27.0 39.0 10-11 34.86954881238057 39.0 35.0 39.0 27.0 39.0 12-13 34.752929000131644 39.0 35.0 39.0 27.0 39.0 14-15 35.70741288522814 39.0 35.5 41.0 26.5 41.0 16-17 35.50527638208783 39.0 35.0 41.0 25.5 41.0 18-19 35.259034926614916 39.0 34.0 41.0 25.0 41.0 20-21 35.050754118367266 39.0 34.0 41.0 24.5 41.0 22-23 34.81226638670839 39.0 34.0 41.0 23.0 41.0 24-25 34.588090588484675 39.0 34.0 41.0 18.5 41.0 26-27 34.266596680181145 39.0 34.0 41.0 17.5 41.0 28-29 34.095181972523676 39.0 34.0 41.0 17.0 41.0 30-31 34.062875847896876 39.0 34.0 41.0 16.0 41.0 32-33 33.94754817512265 39.0 34.0 41.0 15.5 41.0 34-35 33.81000220985598 39.0 33.0 41.0 13.5 41.0 36-37 33.655286144881714 38.0 33.0 41.0 9.5 41.0 38-39 33.53511871628315 38.0 33.0 41.0 9.0 41.0 40-41 33.365082723674185 38.0 33.0 41.0 9.0 41.0 42-43 33.226553460420675 38.0 33.0 40.5 9.0 41.0 44-45 32.989004516491505 38.0 32.5 40.0 8.0 41.0 46-47 32.89745368935657 38.0 32.0 40.0 8.0 41.0 48-49 32.78908040682699 38.0 32.0 40.0 7.0 41.0 50-51 32.30831547858345 37.5 31.0 39.5 2.0 40.5 52-53 32.44559187566486 38.0 31.5 40.0 2.0 41.0 54-55 32.69042446696518 38.0 32.0 40.0 2.0 41.0 56-57 32.58162927409146 38.0 31.5 40.0 2.0 41.0 58-59 32.398496676284665 38.0 31.0 40.0 2.0 41.0 60-61 32.31722096218234 38.0 31.0 40.0 2.0 41.0 62-63 32.09298653937633 38.0 31.0 40.0 2.0 41.0 64-65 31.802134445379675 37.5 31.0 40.0 2.0 41.0 66-67 31.55308691552078 37.0 31.0 40.0 2.0 41.0 68-69 31.216571709394714 36.5 30.0 39.5 2.0 41.0 70-71 30.851239671206255 36.0 30.0 39.0 2.0 41.0 72-73 30.51336213961693 36.0 29.5 39.0 2.0 40.5 74-75 30.09014736188454 35.0 29.0 38.0 2.0 40.0 76-77 29.718113649286693 35.0 29.0 37.0 2.0 39.5 78-79 29.32977080310387 35.0 29.0 37.0 2.0 39.0 80-81 28.910780003268354 35.0 28.0 36.5 2.0 39.0 82-83 28.545817066252212 34.0 28.0 36.0 2.0 38.0 84-85 28.195389809432548 34.0 27.0 36.0 2.0 37.0 86-87 27.799947210881314 34.0 26.0 35.0 2.0 37.0 88-89 27.53029313648596 34.0 26.0 35.0 2.0 36.0 90-91 27.26061714246648 34.0 26.0 35.0 2.0 36.0 92-93 26.93094435293871 34.0 25.5 35.0 2.0 36.0 94-95 26.521819998576536 34.0 25.0 35.0 2.0 35.5 96-97 26.214816458086762 34.0 24.5 35.0 2.0 35.0 98-99 25.831203800458674 34.0 21.5 35.0 2.0 35.0 100-101 24.273894912354976 32.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2067021830372013 1101 2 1.3347035738655038 1101 3 1.4009622060492255 1101 4 1.4471528015314874 1101 5 1.410777019026277 1101 6 1.4610181138246148 1101 7 1.3955012075882465 1101 8 1.3539241932641914 1101 9 1.5518999647117084 1101 10-11 1.4599477357092638 1101 12-13 1.464964634132194 1101 14-15 1.6483994267853035 1101 16-17 1.6619052570917248 1101 18-19 1.7136456468435526 1101 20-21 1.6751751892270548 1101 22-23 1.8074050031486522 1101 24-25 1.7496540384366028 1101 26-27 1.6844959188253483 1101 28-29 1.6562593722966383 1101 30-31 1.6496533967585947 1101 32-33 1.7501359447641534 1101 34-35 1.6875586401413614 1101 36-37 1.6577161248955932 1101 38-39 1.7156614562904267 1101 40-41 1.7681500052056123 1101 42-43 1.7356024407936559 1101 44-45 1.7719436109829019 1101 46-47 1.7552168029330701 1101 48-49 1.6615447521219266 1101 50-51 1.7161269698521053 1101 52-53 1.872898875186884 1101 54-55 1.8262047237307826 1101 56-57 1.8545391050993913 1101 58-59 1.9271098508061897 1101 60-61 1.9845830433296499 1101 62-63 1.9469481010914294 1101 64-65 1.857608181356344 1101 66-67 1.8509954376201208 1101 68-69 1.906605750451316 1101 70-71 1.8707712599057196 1101 72-73 1.9180406506487628 1101 74-75 1.925937787130298 1101 76-77 1.8258216908757774 1101 78-79 1.8708471433521794 1101 80-81 1.9020640503140953 1101 82-83 1.8463254136440916 1101 84-85 1.9194401230679787 1101 86-87 1.9849040668124047 1101 88-89 1.9067936374978487 1101 90-91 1.9414905661736483 1101 92-93 1.8817753127059156 1101 94-95 2.0232978315665626 1101 96-97 2.1107801315024552 1101 98-99 2.15166179206582 1101 100-101 2.266704121514721 1102 1 1.088865607773208 1102 2 1.2504755459847772 1102 3 1.32458628220256 1102 4 1.3627483300603203 1102 5 1.3884671423386905 1102 6 1.305309339730833 1102 7 1.3060974808057964 1102 8 1.3304325366621796 1102 9 1.413374956631138 1102 10-11 1.439394881034218 1102 12-13 1.4826976054524152 1102 14-15 1.6681233649090075 1102 16-17 1.655268783094904 1102 18-19 1.6562563010753166 1102 20-21 1.7216232639707272 1102 22-23 1.680368051379837 1102 24-25 1.7099684648925546 1102 26-27 1.6607645884861526 1102 28-29 1.65104786518652 1102 30-31 1.694609895181543 1102 32-33 1.6958363192586674 1102 34-35 1.716367285688932 1102 36-37 1.6871798725398222 1102 38-39 1.6930771777618077 1102 40-41 1.6899354682962269 1102 42-43 1.6407568295362012 1102 44-45 1.775539812004105 1102 46-47 1.7466427522494854 1102 48-49 1.7847952580644915 1102 50-51 1.7030255246752688 1102 52-53 1.8436227233273712 1102 54-55 1.8584062567319393 1102 56-57 1.809835053092506 1102 58-59 1.9647122614562207 1102 60-61 1.8911020394036058 1102 62-63 1.8600295675023872 1102 64-65 1.9027932629425912 1102 66-67 1.8831160278960581 1102 68-69 1.8855567779469347 1102 70-71 1.9246074178555936 1102 72-73 1.885537328611619 1102 74-75 1.8292193971560486 1102 76-77 1.777561807930006 1102 78-79 1.7889327125434633 1102 80-81 1.8122283262852434 1102 82-83 1.7859635764323478 1102 84-85 1.8455663262166162 1102 86-87 1.8624389435043653 1102 88-89 1.8347117677575397 1102 90-91 1.8416362070088823 1102 92-93 1.8662254077236504 1102 94-95 1.8708498297621787 1102 96-97 1.9824852700381577 1102 98-99 2.055499355484539 1102 100-101 2.1230337946855258 1103 1 1.0155663663558094 1103 2 1.1433772339168584 1103 3 1.1612208925045024 1103 4 1.1706419426102315 1103 5 1.2142563024459463 1103 6 1.2479405662178564 1103 7 1.2199344384749367 1103 8 1.2470141416312046 1103 9 1.3024089356866853 1103 10-11 1.3373261818871782 1103 12-13 1.3641461867055824 1103 14-15 1.6315226189896137 1103 16-17 1.538301903372087 1103 18-19 1.5760794026935159 1103 20-21 1.579235147446255 1103 22-23 1.5176876750242414 1103 24-25 1.5278246991612008 1103 26-27 1.4885753621347106 1103 28-29 1.5687686809034034 1103 30-31 1.5559167174650526 1103 32-33 1.5696933700348055 1103 34-35 1.5546328313293287 1103 36-37 1.5918014038738377 1103 38-39 1.6039518907619907 1103 40-41 1.5931523823110325 1103 42-43 1.549143829791106 1103 44-45 1.646915572870455 1103 46-47 1.6767401194785165 1103 48-49 1.638843910368827 1103 50-51 1.5954417533454617 1103 52-53 1.6748244071268914 1103 54-55 1.7544071807221542 1103 56-57 1.763966279414909 1103 58-59 1.9214941442085731 1103 60-61 1.8068478770745173 1103 62-63 1.7617867598721304 1103 64-65 1.7263033823602392 1103 66-67 1.7232469851718193 1103 68-69 1.6401551700161896 1103 70-71 1.7932443584294582 1103 72-73 1.7947011592092252 1103 74-75 1.7377427851126583 1103 76-77 1.7569132412103698 1103 78-79 1.706494034324642 1103 80-81 1.78661384374605 1103 82-83 1.7842239413423115 1103 84-85 1.777563206838913 1103 86-87 1.7476323282153565 1103 88-89 1.7911774234147728 1103 90-91 1.772874258730944 1103 92-93 1.761030021412676 1103 94-95 1.849308290513946 1103 96-97 1.9407513030680406 1103 98-99 2.00545625555441 1103 100-101 2.059196301195957 1104 1 0.9446503593613258 1104 2 1.055649010101952 1104 3 1.115459433446837 1104 4 1.1194557933599611 1104 5 1.0638421661989952 1104 6 1.0670934257586708 1104 7 1.0872187342720778 1104 8 1.0819840785419501 1104 9 1.1385564006053315 1104 10-11 1.1081796850403123 1104 12-13 1.1967480797646814 1104 14-15 1.3531247267523554 1104 16-17 1.335129337864764 1104 18-19 1.3992211895079336 1104 20-21 1.4051969264178723 1104 22-23 1.4413433304822334 1104 24-25 1.4475999907340196 1104 26-27 1.4355695788170593 1104 28-29 1.4435742802888 1104 30-31 1.4431093277821532 1104 32-33 1.4791386024534816 1104 34-35 1.505984198779153 1104 36-37 1.5202099657566208 1104 38-39 1.4971324554168959 1104 40-41 1.4784665612856713 1104 42-43 1.4971352773991953 1104 44-45 1.6331045357042981 1104 46-47 1.6137246692645348 1104 48-49 1.5353949339582869 1104 50-51 1.4278662644926499 1104 52-53 1.5545911433182695 1104 54-55 1.6455869432980847 1104 56-57 1.6729165242911535 1104 58-59 1.7465896463947743 1104 60-61 1.6647788392898377 1104 62-63 1.566701609018324 1104 64-65 1.5477563530532912 1104 66-67 1.698901502312303 1104 68-69 1.6422293472618357 1104 70-71 1.6321168241089588 1104 72-73 1.621964771088436 1104 74-75 1.677798216656118 1104 76-77 1.6933409666484494 1104 78-79 1.6293240862278466 1104 80-81 1.6586988291534581 1104 82-83 1.5576542220861072 1104 84-85 1.654667548802312 1104 86-87 1.59419769404456 1104 88-89 1.5999354403020476 1104 90-91 1.5919840623039043 1104 92-93 1.6375290969070626 1104 94-95 1.6624921593712472 1104 96-97 1.7096121824317088 1104 98-99 1.82453996195202 1104 100-101 1.8863892465938932 1105 1 0.8437443642237596 1105 2 0.9376845278313368 1105 3 1.0024940840760017 1105 4 1.0712242927494628 1105 5 1.0550541003223657 1105 6 1.077011157078978 1105 7 1.0483053168054113 1105 8 1.011337458989125 1105 9 1.1128030509459563 1105 10-11 1.0900958415931967 1105 12-13 1.0414376597511534 1105 14-15 1.1577193254731952 1105 16-17 1.1308036487276851 1105 18-19 1.1393457413532744 1105 20-21 1.1592689112290557 1105 22-23 1.1670531975016942 1105 24-25 1.2057319366224633 1105 26-27 1.1931049908635174 1105 28-29 1.1908639695165988 1105 30-31 1.2308277775061356 1105 32-33 1.2005407046487662 1105 34-35 1.1748788163975874 1105 36-37 1.209782342475222 1105 38-39 1.2287514251981975 1105 40-41 1.2463368730879125 1105 42-43 1.2612006258419513 1105 44-45 1.332752238722101 1105 46-47 1.294779448489166 1105 48-49 1.3264529700371028 1105 50-51 1.2403749516147826 1105 52-53 1.2595545797057 1105 54-55 1.4458296348590167 1105 56-57 1.4131364832965474 1105 58-59 1.5082494674782225 1105 60-61 1.4655169301136652 1105 62-63 1.44504971868583 1105 64-65 1.4092360390527539 1105 66-67 1.4713778431402744 1105 68-69 1.4208117542972936 1105 70-71 1.4910152331212707 1105 72-73 1.3805825609262428 1105 74-75 1.4702885382333228 1105 76-77 1.4333716577203646 1105 78-79 1.456251108238824 1105 80-81 1.4296785184948142 1105 82-83 1.3823315897323205 1105 84-85 1.4128676767245167 1105 86-87 1.3687457981524176 1105 88-89 1.3704537025192778 1105 90-91 1.3498044322650102 1105 92-93 1.446172708167392 1105 94-95 1.3703869861586213 1105 96-97 1.438280156831766 1105 98-99 1.4326021748261688 1105 100-101 1.5483561485553565 1106 1 0.7295114831721143 1106 2 0.8096949715452695 1106 3 0.8353362220891505 1106 4 0.8936885448834744 1106 5 0.9041195749544357 1106 6 0.8587192985221606 1106 7 0.9064440549570136 1106 8 0.9253254412428191 1106 9 0.9759605217390543 1106 10-11 0.9214949460656499 1106 12-13 0.9411274634410915 1106 14-15 1.0699046245940593 1106 16-17 1.0698535231531494 1106 18-19 1.1384930258392458 1106 20-21 1.0543412483091927 1106 22-23 1.0827072553030064 1106 24-25 1.1513081613667921 1106 26-27 1.1092512804896586 1106 28-29 1.0598495941003634 1106 30-31 1.0543604012314916 1106 32-33 1.0693146879424802 1106 34-35 1.0594049014891596 1106 36-37 1.1470575780578258 1106 38-39 1.1021727941638702 1106 40-41 1.1628966082312573 1106 42-43 1.1193076129761863 1106 44-45 1.2092756119371941 1106 46-47 1.1455004476188648 1106 48-49 1.075399283002831 1106 50-51 1.051307874493176 1106 52-53 1.1398084718384354 1106 54-55 1.1810879652111765 1106 56-57 1.1294038194815883 1106 58-59 1.2410848103249066 1106 60-61 1.219371683898487 1106 62-63 1.1867644555903922 1106 64-65 1.2183063480892322 1106 66-67 1.170595812738199 1106 68-69 1.1742049170914477 1106 70-71 1.2126698863878254 1106 72-73 1.223338274393349 1106 74-75 1.2225810037258817 1106 76-77 1.1908227770292648 1106 78-79 1.1985501484960324 1106 80-81 1.215152042167233 1106 82-83 1.1716256094324393 1106 84-85 1.2472949714694863 1106 86-87 1.229064626677708 1106 88-89 1.1853707798908957 1106 90-91 1.216963518817586 1106 92-93 1.2551354209051837 1106 94-95 1.2190591017212569 1106 96-97 1.1511210669872867 1106 98-99 1.2174631631841386 1106 100-101 1.304874326669097 1107 1 0.5930616586964668 1107 2 0.6828705175194401 1107 3 0.6406755785725906 1107 4 0.7411990773953789 1107 5 0.7618221062100758 1107 6 0.7441161821068292 1107 7 0.7196354466847055 1107 8 0.7264620358406262 1107 9 0.8209031811700456 1107 10-11 0.8292703100436114 1107 12-13 0.8527247611195321 1107 14-15 0.9325068529784986 1107 16-17 0.896456341374325 1107 18-19 0.8394480626140179 1107 20-21 0.9161048386695398 1107 22-23 0.8064355024195962 1107 24-25 0.8949889490973675 1107 26-27 0.889517628296943 1107 28-29 0.8326546452984189 1107 30-31 0.8457224932211744 1107 32-33 0.7863548544405106 1107 34-35 0.7938705084137325 1107 36-37 0.8695162086843808 1107 38-39 0.7841671639514729 1107 40-41 0.8998480297506504 1107 42-43 0.9208020522743681 1107 44-45 0.965166383231221 1107 46-47 0.8251090583954337 1107 48-49 0.7383083106108543 1107 50-51 0.7913024273644425 1107 52-53 0.7841059019470009 1107 54-55 0.9775364275961067 1107 56-57 0.9084655646828352 1107 58-59 0.9764985927944068 1107 60-61 0.9563699493357092 1107 62-63 0.8412805843792341 1107 64-65 0.8760339115105715 1107 66-67 0.9234029447754679 1107 68-69 0.9696259362001207 1107 70-71 0.9813811369634458 1107 72-73 0.9325596006250336 1107 74-75 0.9907990605519608 1107 76-77 0.9605732892312275 1107 78-79 0.9883167625190055 1107 80-81 0.9620692498866532 1107 82-83 0.9484922691060902 1107 84-85 0.9490016029819159 1107 86-87 0.9281165173358623 1107 88-89 1.0063718426273738 1107 90-91 0.973262800260212 1107 92-93 1.0220527505581423 1107 94-95 0.9407032398442965 1107 96-97 1.0141931581364787 1107 98-99 1.1194356173045854 1107 100-101 1.161252652022025 1108 1 0.40577408390301883 1108 2 0.49221685537980875 1108 3 0.5336913441040707 1108 4 0.5343244629435304 1108 5 0.5923223210831949 1108 6 0.5005845255049621 1108 7 0.5763969803175186 1108 8 0.5229485909627485 1108 9 0.49057345172815303 1108 10-11 0.5887772299319067 1108 12-13 0.4724576654144954 1108 14-15 0.5999283125237937 1108 16-17 0.5974550483036083 1108 18-19 0.6275189363336935 1108 20-21 0.5812066345585194 1108 22-23 0.5830487173197767 1108 24-25 0.6161123614692627 1108 26-27 0.5318458757011228 1108 28-29 0.5649263758441023 1108 30-31 0.5597094437210046 1108 32-33 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-0.03851476623437833 2311 58-59 -0.18603415192595207 2311 60-61 -0.27626196370474787 2311 62-63 -0.2032138058981019 2311 64-65 -0.14602724346228158 2311 66-67 -0.21930367649857985 2311 68-69 -0.21004586475962483 2311 70-71 -0.14902505886477257 2311 72-73 -0.09517538075265719 2311 74-75 -0.1961030725681212 2311 76-77 -0.21687440718696038 2311 78-79 -0.17359543294287505 2311 80-81 -0.1173190572114251 2311 82-83 -0.16144419120111664 2311 84-85 -0.15153144033865118 2311 86-87 -0.11192255493935122 2311 88-89 -0.075293920138364 2311 90-91 -0.06220734003342798 2311 92-93 -0.06440063584640754 2311 94-95 -0.036446545958447985 2311 96-97 -0.10616556162409907 2311 98-99 -0.04557999122382128 2311 100-101 0.0899603673918179 2312 1 0.1624077828174464 2312 2 0.23772426036430616 2312 3 0.1171494875678718 2312 4 0.0876550870760866 2312 5 0.08830641224484026 2312 6 0.11376799027221551 2312 7 0.16490994568022188 2312 8 0.11806570960751372 2312 9 0.028236998504752364 2312 10-11 0.020910595308002655 2312 12-13 -0.03571158928595253 2312 14-15 0.015465841745225362 2312 16-17 0.00308420014267341 2312 18-19 -0.02393507831521191 2312 20-21 -0.12784384759955003 2312 22-23 -0.16929049190800072 2312 24-25 0.01030315453709818 2312 26-27 0.0049450554948435865 2312 28-29 -0.0178923907551507 2312 30-31 -0.1611895822063758 2312 32-33 -0.06863978960824113 2312 34-35 0.030146233829384528 2312 36-37 0.06649861754501529 2312 38-39 0.06687068125873452 2312 40-41 0.15796421761503154 2312 42-43 0.1256520391562077 2312 44-45 0.179088730484402 2312 46-47 0.13387617278610975 2312 48-49 0.08483898490080577 2312 50-51 -0.05221270134023115 2312 52-53 -0.13714678037345607 2312 54-55 6.138644249915615E-4 2312 56-57 0.08449119988637932 2312 58-59 -0.010025197335188807 2312 60-61 0.04419709256575999 2312 62-63 0.05466730446264023 2312 64-65 0.01963752645176342 2312 66-67 0.014112636006800017 2312 68-69 -0.003649492621494943 2312 70-71 0.00970973620502491 2312 72-73 -0.003768289058847074 2312 74-75 0.061138154534056355 2312 76-77 -0.01327684679585417 2312 78-79 0.01990671199774141 2312 80-81 0.07388268779874352 2312 82-83 -0.09306750743194314 2312 84-85 0.023475776640083268 2312 86-87 0.1573215524359668 2312 88-89 -0.17720960681636555 2312 90-91 -0.15392882414806763 2312 92-93 0.026620976501252613 2312 94-95 0.07802495629022133 2312 96-97 0.10100411186525449 2312 98-99 0.1376595472850788 2312 100-101 0.1848556322608772 2313 1 -0.5988369066520178 2313 2 -0.4759705805050203 2313 3 -0.9907892962731353 2313 4 -1.0759549045947026 2313 5 -1.185330197937418 2313 6 -0.8454017878764404 2313 7 -0.7664626995572306 2313 8 -0.7005876051820294 2313 9 -0.9338885324759119 2313 10-11 -1.0578908881723237 2313 12-13 -0.9930189557873916 2313 14-15 -1.0434752554054683 2313 16-17 -1.2022858032489339 2313 18-19 -1.0248262927683243 2313 20-21 -1.1165664675513725 2313 22-23 -1.1225617813856346 2313 24-25 -1.085850162031889 2313 26-27 -1.0843337506877049 2313 28-29 -1.0986173570935165 2313 30-31 -1.1149201222369172 2313 32-33 -1.1614215106465267 2313 34-35 -1.1916408841327168 2313 36-37 -1.1005611131227155 2313 38-39 -1.1531637144843643 2313 40-41 -1.1254304178399224 2313 42-43 -1.0985766475416696 2313 44-45 -0.9918154975098048 2313 46-47 -1.050190512154579 2313 48-49 -1.1181111892708593 2313 50-51 -1.069915849743417 2313 52-53 -1.1070576187749097 2313 54-55 -1.2787422002979127 2313 56-57 -1.1213005743328779 2313 58-59 -1.2420980600138662 2313 60-61 -1.151904937343275 2313 62-63 -1.182526446537441 2313 64-65 -1.1223284378262868 2313 66-67 -1.2558310095817724 2313 68-69 -1.1547279165598319 2313 70-71 -1.141217495625444 2313 72-73 -1.2164953295690815 2313 74-75 -1.2586445217417541 2313 76-77 -1.2493242642590374 2313 78-79 -1.2637430391889453 2313 80-81 -1.2520437359390293 2313 82-83 -1.1235478267634207 2313 84-85 -1.0728269069547203 2313 86-87 -1.076367037409657 2313 88-89 -1.162835226920798 2313 90-91 -1.1012549235370237 2313 92-93 -1.0382005113389816 2313 94-95 -1.0405558942548119 2313 96-97 -1.0873871299569622 2313 98-99 -1.047511529038239 2313 100-101 -1.055552041302093 2315 1 -2.5963455043501753 2315 2 -2.2057980010089544 2315 3 -2.315487769525422 2315 4 -2.6556799640843494 2315 5 -3.087071136851428 2315 6 -2.964198232249913 2315 7 -2.64614230108603 2315 8 -2.605473366807022 2315 9 -3.0637922554785995 2315 10-11 -2.9453605302522448 2315 12-13 -3.060647705052773 2315 14-15 -3.5666718925471628 2315 16-17 -3.4156282787465386 2315 18-19 -3.510689751518864 2315 20-21 -3.4039153153161408 2315 22-23 -3.6252638391296195 2315 24-25 -3.5674651707762273 2315 26-27 -3.742797926880126 2315 28-29 -3.947832130706278 2315 30-31 -3.9514105237374864 2315 32-33 -4.058173885559299 2315 34-35 -3.6602952964165603 2315 36-37 -3.5310514450197488 2315 38-39 -3.596412111335958 2315 40-41 -3.610877350508808 2315 42-43 -3.529937981809784 2315 44-45 -3.3690502344610778 2315 46-47 -3.4805581318293264 2315 48-49 -3.484761981863109 2315 50-51 -3.5112212337444006 2315 52-53 -3.5518737077901292 2315 54-55 -3.5783375126306574 2315 56-57 -3.503592877322827 2315 58-59 -3.6255767346429693 2315 60-61 -3.8842760901958755 2315 62-63 -3.865891222407363 2315 64-65 -3.6203801376886453 2315 66-67 -3.7083760987339787 2315 68-69 -3.7028567489090882 2315 70-71 -3.726960393628776 2315 72-73 -3.685376299495214 2315 74-75 -3.5448063387302966 2315 76-77 -3.7321571469781887 2315 78-79 -3.743884894186902 2315 80-81 -3.647625375461999 2315 82-83 -3.6857374041425253 2315 84-85 -3.5987523213664616 2315 86-87 -3.572670086948495 2315 88-89 -3.68231251952281 2315 90-91 -3.506723240921392 2315 92-93 -3.428182374821997 2315 94-95 -3.4048066466998463 2315 96-97 -3.499928272214216 2315 98-99 -3.612175357124233 2315 100-101 -3.56784511757591 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1216521.0 3 315639.0 4 291884.0 5 295576.0 6 346655.0 7 448245.0 8 570774.0 9 712403.0 10 771840.0 11 664379.0 12 530407.0 13 446515.0 14 399623.0 15 378180.0 16 370994.0 17 374328.0 18 386570.0 19 409371.0 20 443137.0 21 487557.0 22 540060.0 23 604620.0 24 677844.0 25 753371.0 26 850894.0 27 960984.0 28 1096499.0 29 1265483.0 30 1471404.0 31 1739239.0 32 2084998.0 33 2562085.0 34 3248795.0 35 4303513.0 36 6104106.0 37 1.0035988E7 38 1.2934244E7 39 3724788.0 40 8241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.549681895233018 15.792369124481938 13.625879248232748 45.03206973205229 2 15.437820469400718 19.905173331612456 49.75423096678836 14.902775232198467 3 19.388554500583417 24.26176155577783 34.18326330281714 22.166420640821617 4 21.063384858671636 31.41052121650077 28.948820301863886 18.577273622963705 5 19.967260845193156 34.66785307422365 30.431717433244078 14.933168647339116 6 15.839784908081365 37.40273336913088 30.848938196310442 15.908543526477306 7 13.13355054914994 20.353761927434288 49.12645447976974 17.386233043646026 8 14.07523493303593 22.360010484790298 41.48528404092156 22.079470541252206 9 15.425080659654405 24.529910548318583 40.34526175282111 19.699747039205903 10-11 18.88529109807459 35.32058787920089 29.457324295742364 16.33679672698215 12-13 17.84997800694954 27.536383022434997 36.32059164551873 18.293047325096733 14-15 18.871668469416974 28.909041893356253 35.23064478720974 16.98864485001703 16-17 19.353768730778853 28.093164619198717 34.06722000163967 18.485846648382758 18-19 19.026557571835383 29.347138284266627 33.54701422698083 18.079289916917165 20-21 19.476406921611115 28.456904213907467 33.013531685965205 19.053157178516212 22-23 19.19225567831013 30.615858432745696 33.04236077952985 17.149525109414324 24-25 18.433848088994893 30.49211591398404 32.516553438333005 18.55748255868806 26-27 18.88209971458148 30.992703253546317 31.71508596228237 18.410111069589835 28-29 18.69635195348783 30.598855740451903 32.180799626074084 18.52399267998618 30-31 18.016872682552425 30.721463109412 32.51470822273457 18.746955985301007 32-33 18.043082404084625 30.423093152979558 32.814013150870636 18.719811292065177 34-35 18.73840613423384 31.50704117802049 31.062077911797015 18.692474775948657 36-37 19.304637438421597 31.02003549733651 31.669555857572025 18.005771206669866 38-39 18.6407339256215 30.56352733393496 32.02853004397077 18.76720869647278 40-41 19.060619366706323 31.066069772598055 30.91261632260618 18.96069453808944 42-43 18.840045921264256 31.182344415719037 31.460105477121363 18.517504185895344 44-45 19.238016013415997 30.62408227156319 31.20487207938715 18.93302963563366 46-47 19.054093745111878 30.22316832642688 31.7494598664915 18.973278061969744 48-49 18.942166568428952 29.897889146320434 31.418916746889607 19.741027538361006 50-51 18.140246962462868 30.606910229736773 32.69001664639984 18.56282616140052 52-53 19.497335409797287 30.465117963633194 31.551542322280557 18.48600430428896 54-55 19.14125804752468 30.625787229691063 31.086311475909305 19.14664324687495 56-57 19.718159294288572 30.54985769531249 30.473888543964506 19.258094466434436 58-59 18.472789713674985 31.646595894710156 30.89425436479513 18.98636002681973 60-61 19.044149335351523 31.25484707376148 30.021999902556797 19.6790036883302 62-63 18.967256374664274 31.540938874175072 30.688913185148515 18.802891566012136 64-65 19.756702664685065 30.816172840767265 30.392983392572088 19.03414110197558 66-67 19.32762305244084 31.456191111063585 30.010829000041266 19.205356836454307 68-69 19.14599551109603 31.431108314958944 30.70426512113246 18.718631052812558 70-71 19.44094253674489 31.3484340947928 30.15994318274157 19.050680185720744 72-73 19.150526699859693 31.30102983103508 30.288767195949916 19.259676273155304 74-75 18.737871351134505 30.433248449632664 32.419386832216254 18.40949336701658 76-77 18.635475751922627 31.229830699919525 31.252249193374688 18.882444354783168 78-79 19.284907196384278 31.289678723634523 30.651895574490922 18.773518505490273 80-81 19.104971028760023 31.038954151206283 30.832554910103312 19.023519909930382 82-83 19.77596763263374 31.451919280833902 29.986703211808813 18.78540987472355 84-85 19.38496933128675 30.843026272449787 30.300947001889966 19.4710573943735 86-87 19.05405378588199 31.925878733841493 30.217475029245307 18.80259245103121 88-89 18.77138650415564 31.232936909353736 30.98031549867793 19.015361087812693 90-91 19.095536096467892 31.693566315305162 30.63055140133253 18.58034618689442 92-93 19.45726011961155 30.653337918206365 30.517906610499036 19.371495351683052 94-95 19.159373446175366 31.00697460790198 30.42088325792126 19.412768688001393 96-97 19.631443925735095 31.041465781523947 30.036843718371447 19.29024657436951 98-99 19.62324385602828 30.798442576051364 30.284175348880677 19.294138219039677 100-101 20.599636482403604 32.032894172985884 28.65117135592527 18.716297988685245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26845.0 1 30603.5 2 29001.0 3 25008.0 4 28117.0 5 31891.0 6 34444.5 7 36759.0 8 40795.5 9 45185.0 10 50198.0 11 56562.0 12 63550.0 13 70715.5 14 78990.0 15 88819.5 16 99874.0 17 112562.0 18 130069.0 19 152262.0 20 184787.0 21 227673.0 22 277619.0 23 335015.5 24 420764.0 25 556870.5 26 816641.5 27 1226818.5 28 1442871.0 29 1501837.0 30 1763069.0 31 2137885.0 32 2384776.5 33 2632584.5 34 3056661.0 35 3636459.0 36 3988834.5 37 4184506.0 38 4104888.0 39 3224390.0 40 2701460.0 41 2591286.5 42 2209772.0 43 1966085.5 44 1838145.5 45 1713867.0 46 1533660.0 47 1376081.5 48 1298323.0 49 1244044.0 50 1128595.0 51 959385.0 52 826953.0 53 724175.0 54 639727.0 55 554207.5 56 476951.0 57 397155.5 58 321831.5 59 255703.0 60 192898.0 61 140380.5 62 102749.5 63 74534.0 64 52464.0 65 36368.5 66 24759.5 67 17641.5 68 12866.0 69 9199.5 70 8137.0 71 8903.5 72 8670.0 73 10026.0 74 10336.5 75 7380.0 76 5876.0 77 5418.0 78 4736.0 79 3480.5 80 2328.5 81 1576.5 82 1179.5 83 886.0 84 735.5 85 703.5 86 632.5 87 468.0 88 334.0 89 227.5 90 186.0 91 162.0 92 122.0 93 76.5 94 49.5 95 36.0 96 19.0 97 7.5 98 6.5 99 4.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.63629845945303 2 0.2755625314429372 3 0.3007631577055716 4 0.2695974936907424 5 0.3018213464560256 6 0.18994642325569386 7 0.09004785203571915 8 0.1037811675536376 9 0.09866607441004356 10-11 0.08671594576606803 12-13 0.09013192096705988 14-15 0.08977636337671054 16-17 0.0811141783502171 18-19 0.10264662261783741 20-21 0.09505033908779255 22-23 0.08807570288490944 24-25 0.10224633110071961 26-27 0.09939801400492758 28-29 0.09231154298512331 30-31 0.10249545279634399 32-33 0.09998418270051435 34-35 0.09661371270089042 36-37 0.10353667350560995 38-39 0.09422199016797651 40-41 0.10346263114406216 42-43 0.08372340032017768 44-45 0.34965271201590603 46-47 0.07251678039007799 48-49 0.06982734586177396 50-51 0.06428342404088225 52-53 0.23164846957369525 54-55 0.2607710271745648 56-57 0.2042566521740056 58-59 0.26359389220857476 60-61 0.07953075159753337 62-63 0.06664583813901681 64-65 0.10651379345951119 66-67 0.09896455768003316 68-69 0.09160042780442462 70-71 0.1003235435242751 72-73 0.12467191135451029 74-75 0.10666650583020354 76-77 0.09912189769832223 78-79 0.0942027083029901 80-81 0.09266710057547266 82-83 0.0998738904327921 84-85 0.09399523543573636 86-87 0.11577680139898106 88-89 0.12565682901801598 90-91 0.14463095543923982 92-93 0.14815413780955608 94-95 0.1098456997291623 96-97 0.09610698528904765 98-99 0.11379925332597517 100-101 0.11045269283893439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.4827754E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.85426015164076 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06608415477434 18.622086804478677 2 8.112813168035588 3.8705031174395548 3 3.6112262709930474 2.5842939280412307 4 2.097776093537473 2.0016358670054224 5 1.3500028777105946 1.6101659925186096 6 0.9701108200880019 1.3884765526980456 7 0.7450050293365145 1.244008064885178 8 0.5654452381426208 1.079062224972843 9 0.4809953386782145 1.0326409146501014 >10 3.3543122101123437 15.833699027138968 >50 0.3729991276571011 6.1554049138294635 >100 0.22691663070657253 10.270444773279198 >500 0.021759234729218258 3.590539743851868 >1k 0.019169135957629334 9.642361634125443 >5k 0.0029708536430014428 4.8859944236730515 >10k+ 0.0024138158982947603 16.188682017412802 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 298634 0.4606576374680511 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 288846 0.44555916590909506 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 282091 0.43513924607044074 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 240157 0.3704539879632418 No Hit CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA 182972 0.2822433120234275 No Hit GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT 139475 0.21514704951832825 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 126927 0.1957911421703735 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 107583 0.1659520704666091 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 107163 0.16530419980306585 No Hit CTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAAC 103079 0.15900442887470698 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 102132 0.15754363478333677 No Hit CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT 94649 0.14600073912787415 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA 84687 0.13063386400830732 No Hit CAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCT 83669 0.12906354892381433 No Hit CCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCC 79519 0.12266196974832723 No Hit CTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATT 75584 0.11659203865060634 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 75383 0.11628198626162493 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 74353 0.11469316058674499 No Hit CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 71779 0.11072263894874408 No Hit CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT 69483 0.10718094598804087 No Hit CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC 66658 0.10282324450111291 No Hit GCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAA 65922 0.10168792829071327 No Hit CACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGC 64932 0.10016080458378983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025527646692803826 3.0850983978251044E-6 0.0 1.5425491989125522E-6 0.0 2 0.025593976308357066 4.627647596737656E-6 0.0 1.5425491989125522E-6 0.0 3 0.025740518482253758 6.170196795650209E-6 0.0 3.0850983978251044E-6 0.0 4 0.026699984083977366 6.170196795650209E-6 0.0 6.170196795650209E-6 0.0 5 0.026905143127432734 7.71274599456276E-6 0.0 1.0797844392387866E-5 0.0 6 0.027145780802463094 7.71274599456276E-6 0.0 1.6968041188038075E-5 0.0 7 0.0273185463127413 7.71274599456276E-6 0.0 2.4680787182600836E-5 0.0 8 0.02802657639504216 7.71274599456276E-6 0.0 2.6223336381513386E-5 0.0 9 0.028271841717669256 7.71274599456276E-6 0.0 3.085098397825104E-5 0.0 10-11 0.029036174845730428 1.0797844392387866E-5 0.0 3.470735697553242E-5 0.0 12-13 0.03023627812248439 1.0797844392387866E-5 0.0 4.3191377569551464E-5 0.0 14-15 0.0315466736669606 1.0797844392387866E-5 0.0 4.8590299765745396E-5 0.0 16-17 0.033182547092407366 1.0797844392387866E-5 0.0 5.707432035976443E-5 0.0 18-19 0.0351770632066013 1.1569118991844143E-5 0.0 7.404236154780251E-5 0.0 20-21 0.03739524895463755 1.2340393591300418E-5 0.0 8.021255834345271E-5 0.0 22-23 0.03975072158137702 1.2340393591300418E-5 0.0 8.484020594019036E-5 0.0 24-25 0.0417159292607916 1.2340393591300418E-5 0.0 9.409550113366569E-5 0.0 26-27 0.04361866369765024 1.2340393591300418E-5 0.0 1.1491991531898513E-4 0.0 28-29 0.0457219295303675 1.2340393591300418E-5 0.0 1.2648903431082927E-4 0.0 30-31 0.04797559390997874 1.542549198912552E-5 0.0 1.5039854689397384E-4 0.0 32-33 0.050266279470363884 2.1595688784775732E-5 0.0 1.5425491989125523E-4 0.0 34-35 0.052836166435752195 2.1595688784775732E-5 0.0 1.5888256748799288E-4 0.0 36-37 0.0555402551814459 2.3138237983688282E-5 0.0 1.7045168647983702E-4 0.0 38-39 0.05837623188364663 2.3138237983688282E-5 0.0 1.812495308722249E-4 0.0 40-41 0.06155773960640376 2.545206178205711E-5 1.5425491989125522E-6 1.9127610066515646E-4 2.3138237983688285E-6 42-43 0.06506009756253471 2.776588558042594E-5 1.5425491989125522E-6 2.059303180548257E-4 3.0850983978251044E-6 44-45 0.0690290766513367 2.9308434779338493E-5 1.5425491989125522E-6 2.128717894499322E-4 3.0850983978251044E-6 46-47 0.07354566070575266 2.9308434779338493E-5 1.5425491989125522E-6 2.221270846434075E-4 3.0850983978251044E-6 48-49 0.07824580811483921 2.9308434779338493E-5 1.5425491989125522E-6 2.2521218304123261E-4 3.0850983978251044E-6 50-51 0.0835043583339321 3.085098397825104E-5 1.5425491989125522E-6 2.3138237983688284E-4 3.0850983978251044E-6 52-53 0.08974319856893392 3.239353317716359E-5 1.5425491989125522E-6 2.3755257663253304E-4 3.85637299728138E-6 54-55 0.09667387211964801 3.54786315749887E-5 1.5425491989125522E-6 2.4140894962981443E-4 6.170196795650209E-6 56-57 0.10443058076637979 3.54786315749887E-5 1.5425491989125522E-6 2.4912169562437715E-4 6.170196795650209E-6 58-59 0.11354241888435623 3.54786315749887E-5 1.5425491989125522E-6 2.5914826541730874E-4 6.170196795650209E-6 60-61 0.12332449462926018 3.779245537335753E-5 1.5425491989125522E-6 2.660897368124153E-4 6.170196795650209E-6 62-63 0.1345619655433381 3.933500457227008E-5 1.5425491989125522E-6 2.761163066053468E-4 6.170196795650209E-6 64-65 0.14745382047324979 4.010627917172636E-5 1.5425491989125522E-6 2.853716017988221E-4 6.170196795650209E-6 66-67 0.16348630557214738 4.010627917172636E-5 1.5425491989125522E-6 2.899992493955598E-4 7.71274599456276E-6 68-69 0.181459317563277 4.164882837063891E-5 1.5425491989125522E-6 2.9616944619121E-4 7.71274599456276E-6 70-71 0.20135280330705274 4.396265216900774E-5 1.5425491989125522E-6 3.015683683874039E-4 7.71274599456276E-6 72-73 0.22350689490183479 4.8590299765745396E-5 1.5425491989125522E-6 3.1082366358087927E-4 7.71274599456276E-6 74-75 0.25013129407506546 5.090412356411422E-5 1.5425491989125522E-6 3.2007895877435457E-4 7.71274599456276E-6 76-77 0.282031211508577 5.090412356411422E-5 1.5425491989125522E-6 3.4167464755913027E-4 7.71274599456276E-6 78-79 0.31792324626887425 5.398922196193933E-5 1.5425491989125522E-6 3.4938739355369305E-4 7.71274599456276E-6 80-81 0.3576122658822948 5.7074320359764435E-5 1.5425491989125522E-6 3.571001395482558E-4 7.71274599456276E-6 82-83 0.40683346827039546 6.0159418757589535E-5 1.5425491989125522E-6 3.648128855428186E-4 7.71274599456276E-6 84-85 0.46455797311750147 6.0159418757589535E-5 1.5425491989125522E-6 3.732969061368376E-4 8.484020594019036E-6 86-87 0.5332268645308922 6.0159418757589535E-5 1.5425491989125522E-6 3.825522013303129E-4 9.255295193475312E-6 88-89 0.6110947480919977 6.170196795650209E-5 1.5425491989125522E-6 3.8640857432759436E-4 9.255295193475312E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 45130 0.0 72.69425 1 GCGTGTT 62875 0.0 51.90868 2 CGTGTTT 86090 0.0 38.57936 3 CCTTTAG 87370 0.0 36.96662 5 TTAGGCA 94035 0.0 34.212513 8 TAGGCAT 95060 0.0 33.743267 9 GTTTTTT 711115 0.0 33.259487 1 ACTCGTC 93245 0.0 33.0383 42-43 TCCTTTA 100820 0.0 32.33303 4 TCGTCTA 95300 0.0 32.255573 44-45 CTAACGA 98915 0.0 29.959118 94-95 CTTTAGG 109105 0.0 29.769714 6 GTCTATG 104180 0.0 29.633987 46-47 CTTTCCT 120365 0.0 28.314417 1 ATGTGGC 109040 0.0 28.314205 50-51 CTATGTG 113295 0.0 27.393917 48-49 TTTAGGC 122940 0.0 27.307236 7 GTGGCAA 113820 0.0 27.242262 52-53 GCCTAAC 110260 0.0 27.157595 92-93 CCCGAAA 116735 0.0 26.996277 1 >>END_MODULE