FastQCFastQC Report
Fri 27 May 2016
SRR1233037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64827754
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA2772130.42761469107814537No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT2709020.4178796630838082No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA2213400.3414278396873043No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC1663920.25666784630545736No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA1611910.24864504792191322No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT1517340.2340571601477972No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT1107590.17085120672235538No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1103570.1702311019443925No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT1000630.1543521004907867No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG976310.15060062083903136No Hit
CAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCT824880.1272417983198986No Hit
CTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAAC808210.12467036880531139No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA795560.122719044068687No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC772410.11914804267320445No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC766040.11816543883349714No Hit
CCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCC717880.1107365218915343No Hit
CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT709300.10941301467886733No Hit
CAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCAT693000.10689865948463985No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC688010.1061289274343825No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT682970.10535148263813059No Hit
CAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCG661010.10196404459731861No Hit
GGAAAAGTCAGATTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA657620.10144112041888727No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA654340.10093516428164392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT456200.071.7614441
GCGTGTT639700.051.174912
CGTGTTT871500.038.037983
CCTTTAG811500.033.025985
ACTCGTC930850.032.68467342-43
TCGTCTA949800.031.95735744-45
TTAGGCA864900.030.6899938
TAGGCAT880500.030.0496839
GTCTATG1032800.029.45528846-47
CTAACGA998750.029.42483394-95
CCCGTAT87150.029.315011
GTTTTTT6964450.029.073371
TCCTTTA913700.028.3185634
ATGTGGC1087350.027.814850-51
CCCGAAA1041150.027.67171
CTATGTG1120450.027.09738248-49
GCCTAAC1095250.026.8806492-93
GTGGCAA1134350.026.78856752-53
CTTTAGG1006250.026.6862816
GAGGTGA1099650.026.65639182-83
Produced by FastQC (version 0.11.5)