##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233037_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64827754 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.628864159014363 31.0 30.0 34.0 25.0 34.0 2 30.031503497714883 31.0 30.0 34.0 26.0 34.0 3 28.89088437338119 31.0 28.0 34.0 16.0 34.0 4 31.939622696168065 35.0 32.0 37.0 19.0 37.0 5 32.66362815839648 35.0 33.0 37.0 25.0 37.0 6 33.037446507864516 35.0 33.0 37.0 27.0 37.0 7 33.312612218526034 36.0 35.0 37.0 28.0 37.0 8 33.31403304516766 37.0 35.0 37.0 28.0 37.0 9 34.75918648978646 39.0 35.0 39.0 27.0 39.0 10-11 34.636422920035145 39.0 35.0 39.0 27.0 39.0 12-13 34.52010369663586 39.0 35.0 39.0 27.0 39.0 14-15 35.58726287972278 40.0 36.0 41.0 27.0 41.0 16-17 35.42047952178013 39.5 36.0 41.0 26.0 41.0 18-19 35.23339580760425 39.5 35.0 41.0 25.0 41.0 20-21 35.03980840520867 39.0 35.0 41.0 24.0 41.0 22-23 34.862438454986425 39.0 35.0 41.0 22.5 41.0 24-25 34.6280693065504 39.0 34.0 41.0 18.5 41.0 26-27 34.313725044369114 39.0 34.0 41.0 17.0 41.0 28-29 34.15442053568599 39.0 34.0 41.0 15.0 41.0 30-31 33.96003214148064 39.0 34.0 41.0 10.0 41.0 32-33 33.76497033970975 39.0 34.0 41.0 9.0 41.0 34-35 33.608406794410925 39.0 33.0 41.0 8.5 41.0 36-37 33.53118253179032 39.0 33.0 40.5 8.0 41.0 38-39 33.56154662553942 39.0 33.0 41.0 4.5 41.0 40-41 33.508069776719395 39.0 33.0 41.0 2.0 41.0 42-43 33.43499062916787 39.0 33.0 41.0 2.0 41.0 44-45 33.349218337874234 39.0 33.0 41.0 2.0 41.0 46-47 33.19738660080681 39.0 33.0 41.0 2.0 41.0 48-49 33.100450518461585 39.0 33.0 41.0 2.0 41.0 50-51 33.01454702564584 39.0 33.0 41.0 2.0 41.0 52-53 32.851102847092314 38.5 32.5 40.5 2.0 41.0 54-55 32.70325217498666 38.0 32.0 40.0 2.0 41.0 56-57 32.550411950103964 38.0 32.0 40.0 2.0 41.0 58-59 32.415025484301054 38.0 32.0 40.0 2.0 41.0 60-61 32.36703808217696 38.0 32.0 40.0 2.0 41.0 62-63 32.17853042541008 38.0 32.0 40.0 2.0 41.0 64-65 31.742941449120696 37.5 31.0 40.0 2.0 41.0 66-67 31.563614960345532 37.0 31.0 40.0 2.0 41.0 68-69 31.17689477565427 36.5 31.0 39.5 2.0 41.0 70-71 30.877328281340738 36.0 30.0 39.0 2.0 41.0 72-73 30.58309489636183 36.0 30.0 39.0 2.0 40.5 74-75 30.205232306829572 35.0 30.0 38.5 2.0 40.0 76-77 29.058500823582442 34.0 28.5 36.5 2.0 39.0 78-79 29.51002570596538 35.0 29.0 37.0 2.0 39.0 80-81 29.318054008164466 35.0 29.0 36.5 2.0 39.0 82-83 29.005010585435368 35.0 29.0 36.0 2.0 38.5 84-85 28.712015906026917 35.0 29.0 36.0 2.0 37.0 86-87 28.34204632478861 34.0 29.0 35.0 2.0 37.0 88-89 28.08967991548805 34.0 29.0 35.0 2.0 36.5 90-91 27.838052387253768 34.0 29.0 35.0 2.0 36.0 92-93 27.584244388599366 34.0 28.0 35.0 2.0 36.0 94-95 27.310887733361856 34.0 27.0 35.0 2.0 36.0 96-97 27.05783533700705 34.0 26.5 35.0 2.0 35.0 98-99 26.73141365502189 34.0 26.0 35.0 2.0 35.0 100-101 25.82020235499752 33.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8630768655036185 1101 2 1.7509697438418854 1101 3 2.917128752440931 1101 4 3.251225830370778 1101 5 2.249504767063982 1101 6 1.8636658707824267 1101 7 1.651294348384809 1101 8 1.6142327865636474 1101 9 1.6981828184753383 1101 10-11 1.743077754520641 1101 12-13 1.7055106545499328 1101 14-15 1.8688645082168662 1101 16-17 1.927607774401423 1101 18-19 1.8994091728037006 1101 20-21 1.832186099797397 1101 22-23 1.7928973356965159 1101 24-25 1.9050197631458303 1101 26-27 1.785784432739419 1101 28-29 1.9147089899108636 1101 30-31 1.9247982562026706 1101 32-33 1.915642706454932 1101 34-35 1.9592104875833343 1101 36-37 1.898768230849086 1101 38-39 1.876340804618117 1101 40-41 1.937942157318922 1101 42-43 1.9471778606572485 1101 44-45 1.9543416788971726 1101 46-47 1.9120843964688987 1101 48-49 1.8885192487300415 1101 50-51 1.8909962951578194 1101 52-53 1.8955540604636454 1101 54-55 1.9039436866258939 1101 56-57 1.863658171261008 1101 58-59 1.8301993168811492 1101 60-61 1.7642228214007503 1101 62-63 1.9232319396784519 1101 64-65 1.843481366320006 1101 66-67 1.9233058431665668 1101 68-69 1.8539825661367075 1101 70-71 1.877406612505208 1101 72-73 1.9420496155347493 1101 74-75 1.8794776287460913 1101 76-77 1.7497670294249978 1101 78-79 1.909160601778165 1101 80-81 1.9248449330736825 1101 82-83 1.9190057848041029 1101 84-85 1.9720693899978947 1101 86-87 1.9182862465184591 1101 88-89 1.8829544642210756 1101 90-91 1.9496539052558894 1101 92-93 2.034849081698365 1101 94-95 2.0263163400587025 1101 96-97 2.0846315720874493 1101 98-99 2.120143062941846 1101 100-101 2.1583731860302606 1102 1 1.7709427121910934 1102 2 1.6535061986271309 1102 3 2.840448581298876 1102 4 3.1968611241408595 1102 5 2.1909520743936994 1102 6 1.7833382413975727 1102 7 1.5401856537244925 1102 8 1.4992664776097797 1102 9 1.5777424535324158 1102 10-11 1.6349369422313202 1102 12-13 1.6727994186131454 1102 14-15 1.845475764186837 1102 16-17 1.8067071680132898 1102 18-19 1.796368046320417 1102 20-21 1.7582145741329924 1102 22-23 1.842320273830893 1102 24-25 1.8881212445956521 1102 26-27 1.8471037023378898 1102 28-29 1.7436208159260573 1102 30-31 1.7949867841593559 1102 32-33 1.8007597213509783 1102 34-35 1.8102240535042853 1102 36-37 1.8552615189923998 1102 38-39 1.911938402018066 1102 40-41 1.8990649571439704 1102 42-43 1.8795616208230825 1102 44-45 1.8811614826699525 1102 46-47 1.9370962529271765 1102 48-49 1.8888366952396325 1102 50-51 1.87026731154954 1102 52-53 1.9311595815438451 1102 54-55 1.919944244749118 1102 56-57 1.9286494294720242 1102 58-59 1.863308579978451 1102 60-61 1.8375039550704528 1102 62-63 1.9133061419656272 1102 64-65 1.8719992220675081 1102 66-67 1.898002414198416 1102 68-69 1.8243582312215239 1102 70-71 1.7541202498446005 1102 72-73 1.7965287538494934 1102 74-75 1.8396000688567788 1102 76-77 1.7549362815687317 1102 78-79 1.8467932494559705 1102 80-81 1.864350305932856 1102 82-83 1.8774472369750725 1102 84-85 1.8875498472944514 1102 86-87 1.80340391476523 1102 88-89 1.6613295829386132 1102 90-91 1.7500363166785746 1102 92-93 1.8207246210514079 1102 94-95 1.8337493995790837 1102 96-97 1.8811319238479811 1102 98-99 1.8707943611825115 1102 100-101 1.8435148219785162 1103 1 1.729495057923213 1103 2 1.5897286184650774 1103 3 2.768737421374233 1103 4 3.1205681461510366 1103 5 2.181376961306114 1103 6 1.7584680992590123 1103 7 1.522292966566738 1103 8 1.4288145511483847 1103 9 1.5929423871480353 1103 10-11 1.5897270515957231 1103 12-13 1.610858942942457 1103 14-15 1.7436870411476164 1103 16-17 1.7850120663456366 1103 18-19 1.7563029704240805 1103 20-21 1.7520697109309253 1103 22-23 1.791216906868037 1103 24-25 1.8097586653262212 1103 26-27 1.8493306198600052 1103 28-29 1.7895971512280227 1103 30-31 1.8219502803009178 1103 32-33 1.827360541828547 1103 34-35 1.8358303451598772 1103 36-37 1.8128599950617215 1103 38-39 1.8571291776180914 1103 40-41 1.769821767132278 1103 42-43 1.7945043108181835 1103 44-45 1.8644225214179286 1103 46-47 1.8745131886785487 1103 48-49 1.9094947784275718 1103 50-51 1.9012694318159618 1103 52-53 1.882592285731235 1103 54-55 1.8691972165372306 1103 56-57 1.90104707106768 1103 58-59 1.8908363211654304 1103 60-61 1.9067881898833434 1103 62-63 1.954590762059432 1103 64-65 1.9076353526019432 1103 66-67 1.9016447707007984 1103 68-69 1.8661590164337127 1103 70-71 1.8685336320921913 1103 72-73 1.8262383967384963 1103 74-75 1.8376458790505659 1103 76-77 1.744059727449173 1103 78-79 1.7898004964565608 1103 80-81 1.8010070426980391 1103 82-83 1.8052712967400453 1103 84-85 1.8028604155628791 1103 86-87 1.7638264204895044 1103 88-89 1.527847695788175 1103 90-91 1.573350867054014 1103 92-93 1.5927895620208297 1103 94-95 1.6353458528280278 1103 96-97 1.6358147447555424 1103 98-99 1.6203986556138972 1103 100-101 1.624428591880779 1104 1 1.6372903496863138 1104 2 1.5062813807671525 1104 3 2.6690108636496674 1104 4 3.0613451866442105 1104 5 2.015772185792578 1104 6 1.6469708816559319 1104 7 1.441488141606854 1104 8 1.3304657829667832 1104 9 1.4435005935672223 1104 10-11 1.4756648505227759 1104 12-13 1.5264204842113216 1104 14-15 1.6617678639742977 1104 16-17 1.6532155261865569 1104 18-19 1.6272730561883648 1104 20-21 1.6139020281955396 1104 22-23 1.5457651732893751 1104 24-25 1.6277841272795044 1104 26-27 1.6290628234190194 1104 28-29 1.6334273334511167 1104 30-31 1.6702268297432283 1104 32-33 1.645091740733072 1104 34-35 1.659293996762898 1104 36-37 1.7032056837152254 1104 38-39 1.7364804537436669 1104 40-41 1.690602235318849 1104 42-43 1.6451442667725473 1104 44-45 1.6378029759596373 1104 46-47 1.6439070714450281 1104 48-49 1.6177603807940102 1104 50-51 1.6014941722727727 1104 52-53 1.6456983856099328 1104 54-55 1.7124714981872486 1104 56-57 1.681707110422039 1104 58-59 1.6708544700247927 1104 60-61 1.670136621667055 1104 62-63 1.6899377144134817 1104 64-65 1.6460755556334306 1104 66-67 1.7236089086157307 1104 68-69 1.664783616606023 1104 70-71 1.6569275503714174 1104 72-73 1.6258370933719348 1104 74-75 1.6788109609107487 1104 76-77 1.5832690681620427 1104 78-79 1.638712353159022 1104 80-81 1.6649026046312478 1104 82-83 1.7009829587014238 1104 84-85 1.6777395506916015 1104 86-87 1.6571012701666952 1104 88-89 1.6337571690296926 1104 90-91 1.6900756697579524 1104 92-93 1.7072167373077711 1104 94-95 1.6775231389893293 1104 96-97 1.6031873957463247 1104 98-99 1.717045172386456 1104 100-101 1.7712496705677907 1105 1 1.4102111798389707 1105 2 1.2783313462250092 1105 3 2.4429118746871694 1105 4 2.8361940726637513 1105 5 1.7293810902697615 1105 6 1.2604706317299588 1105 7 1.0381388710761712 1105 8 0.9327383366350048 1105 9 1.0021593055037457 1105 10-11 1.0713509061931106 1105 12-13 1.1412934068675966 1105 14-15 1.3124825200478583 1105 16-17 1.2901088471105595 1105 18-19 1.2764741966919573 1105 20-21 1.2831164800791512 1105 22-23 1.2776760470992201 1105 24-25 1.3318077526803407 1105 26-27 1.2706117254353728 1105 28-29 1.250611382205797 1105 30-31 1.2541577415210767 1105 32-33 1.2368265519781616 1105 34-35 1.1999338765233318 1105 36-37 1.2803328392061601 1105 38-39 1.322234143169105 1105 40-41 1.2659209361288575 1105 42-43 1.2987352105236027 1105 44-45 1.2621664685526 1105 46-47 1.3349711605756553 1105 48-49 1.3072618095232684 1105 50-51 1.3001735769719005 1105 52-53 1.3068109668437344 1105 54-55 1.2126050321011235 1105 56-57 1.251182966893012 1105 58-59 1.2893564644193987 1105 60-61 1.2854107573548461 1105 62-63 1.2526015662633299 1105 64-65 1.23427867903726 1105 66-67 1.3256237401790827 1105 68-69 1.3213978396495492 1105 70-71 1.2954657843726203 1105 72-73 1.2363923143927735 1105 74-75 1.2881541041424711 1105 76-77 1.236890772400507 1105 78-79 1.3646356587313058 1105 80-81 1.38095319687665 1105 82-83 1.345647741394444 1105 84-85 1.3737043881703919 1105 86-87 1.3413951469354792 1105 88-89 1.3594571701374178 1105 90-91 1.377966536909799 1105 92-93 1.4094035820162993 1105 94-95 1.4180262051403965 1105 96-97 1.3751256601677753 1105 98-99 1.4271497630376828 1105 100-101 1.4341471339443714 1106 1 1.3509759158720698 1106 2 1.2128635609847862 1106 3 2.4323740000916168 1106 4 2.8341039271483766 1106 5 1.8165143613621026 1106 6 1.231485769421944 1106 7 1.0803107859305783 1106 8 0.9300478619818051 1106 9 1.0061128022028427 1106 10-11 1.0710012638196815 1106 12-13 1.1113381093106511 1106 14-15 1.1770912085421728 1106 16-17 1.175826902800651 1106 18-19 1.0780662510173613 1106 20-21 1.0629898606841195 1106 22-23 0.997111394918015 1106 24-25 1.0486131140099033 1106 26-27 1.044039540525688 1106 28-29 1.0991163077358763 1106 30-31 1.0624007947446756 1106 32-33 0.9544953049863025 1106 34-35 1.0445370683067807 1106 36-37 1.0604766760664148 1106 38-39 1.0688744501883676 1106 40-41 1.0208407312334273 1106 42-43 1.0445606297214454 1106 44-45 1.076513978564634 1106 46-47 1.0164317522379491 1106 48-49 1.0871179963291269 1106 50-51 1.1363042572608464 1106 52-53 1.139252077376959 1106 54-55 1.2269141226008742 1106 56-57 1.2679115831173249 1106 58-59 1.2540681867569958 1106 60-61 1.2518893216738505 1106 62-63 1.2651609501939802 1106 64-65 1.2240566369681432 1106 66-67 1.2617943582038151 1106 68-69 1.243205591267781 1106 70-71 1.2447777542088367 1106 72-73 1.2444802189984756 1106 74-75 1.2386715911478383 1106 76-77 1.1024961001375324 1106 78-79 1.2031115411346143 1106 80-81 1.2467686028058083 1106 82-83 1.224424963909719 1106 84-85 1.2503305980482224 1106 86-87 1.2570835481278806 1106 88-89 1.2093773262503191 1106 90-91 1.2254703546610948 1106 92-93 1.1998075850207748 1106 94-95 1.1924427564036186 1106 96-97 1.2231584995417037 1106 98-99 1.216554360099444 1106 100-101 1.2115354866252446 1107 1 1.1546612232713187 1107 2 1.0767818825307636 1107 3 2.2087426388333427 1107 4 2.6205137337629125 1107 5 1.579287624014043 1107 6 1.1391413797138341 1107 7 0.9827886863052768 1107 8 0.9160652451769167 1107 9 0.943623292612763 1107 10-11 0.9233037484472035 1107 12-13 0.9013702695812782 1107 14-15 0.9593507890662565 1107 16-17 0.908186773660546 1107 18-19 0.9082501286629991 1107 20-21 0.8948455147627783 1107 22-23 0.8890964343568513 1107 24-25 0.8547286693246434 1107 26-27 0.8182510741154729 1107 28-29 0.8818960918046201 1107 30-31 0.8850905249533625 1107 32-33 0.870398616041129 1107 34-35 0.8813462590074437 1107 36-37 0.8358411526219527 1107 38-39 0.8267612260891539 1107 40-41 0.8309123051805969 1107 42-43 0.8911784407079324 1107 44-45 0.8732672209354106 1107 46-47 0.7784241333593371 1107 48-49 0.8517830396788568 1107 50-51 0.8174324923589822 1107 52-53 0.8259321072084305 1107 54-55 0.7181133326450251 1107 56-57 0.7590529030276514 1107 58-59 0.8493655214273588 1107 60-61 0.8377928738453804 1107 62-63 0.9329723688051601 1107 64-65 0.8251691863637518 1107 66-67 0.8378287912373352 1107 68-69 0.824550899359707 1107 70-71 0.8269726783102556 1107 72-73 0.8525091289257531 1107 74-75 0.8566085430348949 1107 76-77 0.7509316335933818 1107 78-79 0.8398465445505181 1107 80-81 0.8827584748784112 1107 82-83 0.8910532826899384 1107 84-85 0.8375830557742034 1107 86-87 0.8820548088546651 1107 88-89 0.8200098192478862 1107 90-91 0.8293088394819357 1107 92-93 0.8006888119828979 1107 94-95 0.7642504692683723 1107 96-97 0.7639580511669024 1107 98-99 0.8406088764013617 1107 100-101 0.8393824542227577 1108 1 1.1520758641977054 1108 2 1.0529362437365002 1108 3 2.1653723905409343 1108 4 2.627855760110201 1108 5 1.473945190992545 1108 6 1.0541014264071293 1108 7 0.8315248148545678 1108 8 0.8125719903086619 1108 9 0.7735552972268493 1108 10-11 0.772010266579052 1108 12-13 0.8241653173527226 1108 14-15 0.8092989648482032 1108 16-17 0.8312298336261534 1108 18-19 0.7987231185575396 1108 20-21 0.8102163551038686 1108 22-23 0.826553356947521 1108 24-25 0.8418848853128793 1108 26-27 0.9450367583208887 1108 28-29 0.8893341983281999 1108 30-31 0.8764415687066034 1108 32-33 0.8762305589994455 1108 34-35 0.8346226169691988 1108 36-37 0.8382941150488179 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-0.3258185417980215 2311 96-97 -0.2913427369828767 2311 98-99 -0.2786061650434313 2311 100-101 -0.23668172057364245 2312 1 0.3679654218724977 2312 2 0.4639573328114679 2312 3 1.7063308544090496 2312 4 1.7502252252100305 2312 5 0.7173857394495045 2312 6 0.4415729094276699 2312 7 0.2953385157729471 2312 8 0.235312155400635 2312 9 0.1640879981678438 2312 10-11 0.1577602155086879 2312 12-13 0.1552883938547751 2312 14-15 -0.05928056356577116 2312 16-17 -0.005111119763313354 2312 18-19 -0.5402362664909575 2312 20-21 -0.5608745421046848 2312 22-23 -0.21656287980830058 2312 24-25 0.05645733255369123 2312 26-27 0.12625509956687608 2312 28-29 0.057692278118217644 2312 30-31 -0.41478437957456293 2312 32-33 -0.5186260098590267 2312 34-35 -0.3859336277800054 2312 36-37 -0.24223851708793376 2312 38-39 -0.1494551387492109 2312 40-41 -0.6508581744368414 2312 42-43 -0.35158102276758996 2312 44-45 -0.10298733914036262 2312 46-47 -0.254742649170538 2312 48-49 -0.03634159859340258 2312 50-51 -0.026442166792932653 2312 52-53 -0.3921296513493715 2312 54-55 -0.10820422039335398 2312 56-57 -0.059377838654000925 2312 58-59 0.01909742400580683 2312 60-61 0.024332606554573033 2312 62-63 -0.04849014144740238 2312 64-65 0.06941801113713097 2312 66-67 -0.16937046211506157 2312 68-69 -0.007746321745639051 2312 70-71 0.028713506290383606 2312 72-73 0.0650791244051021 2312 74-75 -0.051168012730194334 2312 76-77 -0.36273357853830746 2312 78-79 -0.16753575460266745 2312 80-81 -0.127899062112661 2312 82-83 -0.1214271223457537 2312 84-85 -0.11601633151260415 2312 86-87 -0.11468029530622559 2312 88-89 -0.09582822333976893 2312 90-91 -0.13156581991879435 2312 92-93 -0.2144136409093953 2312 94-95 -0.25883533751848375 2312 96-97 -0.34775311356458616 2312 98-99 -0.35401646264847386 2312 100-101 -0.4305805013430799 2313 1 -1.4238022939910735 2313 2 -0.9150364844338839 2313 3 -3.860334735133513 2313 4 -4.961915855626614 2313 5 -2.904165131091027 2313 6 -2.5843140548094574 2313 7 -1.6952269521446937 2313 8 -1.299492845979188 2313 9 -1.2906847911627963 2313 10-11 -1.199736089277856 2313 12-13 -1.189595247721705 2313 14-15 -1.3002469426003103 2313 16-17 -1.4989032325420624 2313 18-19 -1.418821869723665 2313 20-21 -1.3136520332746073 2313 22-23 -1.7279796907048208 2313 24-25 -1.4519886460110953 2313 26-27 -1.911517682356461 2313 28-29 -1.9976037936172588 2313 30-31 -1.6334362743803936 2313 32-33 -1.6766964474451456 2313 34-35 -1.611436105069636 2313 36-37 -1.9168909827742908 2313 38-39 -2.4983992310029066 2313 40-41 -2.264768143625677 2313 42-43 -1.8290659710078145 2313 44-45 -1.6829253332364829 2313 46-47 -1.5009253440688965 2313 48-49 -1.551891728654141 2313 50-51 -1.5360624206226632 2313 52-53 -1.9310404102303096 2313 54-55 -1.7076918962547225 2313 56-57 -1.6960977490714662 2313 58-59 -1.6139516445368578 2313 60-61 -1.6916224008779785 2313 62-63 -1.621377786738961 2313 64-65 -1.5072795778065249 2313 66-67 -1.7720810444504451 2313 68-69 -1.6823595545153864 2313 70-71 -1.663360576376128 2313 72-73 -1.6431569680834741 2313 74-75 -1.487138988354058 2313 76-77 -1.4077998771649725 2313 78-79 -1.533870427171557 2313 80-81 -1.5616867411473407 2313 82-83 -1.6019667452900208 2313 84-85 -1.585816059147028 2313 86-87 -1.5940670229766276 2313 88-89 -1.4745907342219553 2313 90-91 -1.5039507352691714 2313 92-93 -1.4153175478489182 2313 94-95 -1.396614804134682 2313 96-97 -1.4012791170276806 2313 98-99 -1.3548678075102103 2313 100-101 -1.3296621167743687 2315 1 -4.045347719844177 2315 2 -5.867275153132237 2315 3 -5.158672384268893 2315 4 -5.4329406015277435 2315 5 -4.205283203612833 2315 6 -3.5773368610162244 2315 7 -3.224694303092523 2315 8 -3.117515336492932 2315 9 -3.5132105402685596 2315 10-11 -3.3733622250251862 2315 12-13 -3.4088541231837546 2315 14-15 -3.636792034062527 2315 16-17 -3.632123209461355 2315 18-19 -3.6510787909640356 2315 20-21 -3.664716166149283 2315 22-23 -3.703544992518843 2315 24-25 -3.751551117484773 2315 26-27 -3.67960016632626 2315 28-29 -3.7266404541952944 2315 30-31 -3.7907526796686 2315 32-33 -3.9174283952005595 2315 34-35 -3.920493710479235 2315 36-37 -3.791658995283065 2315 38-39 -3.788256968610721 2315 40-41 -3.7743378053329764 2315 42-43 -3.772544721598713 2315 44-45 -3.779923123888249 2315 46-47 -3.8707422544902457 2315 48-49 -3.9002364833398175 2315 50-51 -3.791581973709331 2315 52-53 -3.811051287851164 2315 54-55 -3.7553697372586825 2315 56-57 -3.8131132805618577 2315 58-59 -3.8034592254266286 2315 60-61 -3.8388061736327543 2315 62-63 -3.8730319615853332 2315 64-65 -3.7281121263757058 2315 66-67 -3.7748620061162086 2315 68-69 -3.7923071016608283 2315 70-71 -3.8230347998087737 2315 72-73 -3.8649760775997635 2315 74-75 -3.870157404097231 2315 76-77 -3.7748265083148524 2315 78-79 -3.8846268321603503 2315 80-81 -3.918323076240682 2315 82-83 -3.8571298039137574 2315 84-85 -3.8722262242054413 2315 86-87 -4.0028807677898115 2315 88-89 -4.00791884195673 2315 90-91 -4.05568350991588 2315 92-93 -3.972427336671423 2315 94-95 -3.974595152099244 2315 96-97 -3.92601379648503 2315 98-99 -3.7724325845965936 2315 100-101 -3.80541954189896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2331860.0 3 619880.0 4 355171.0 5 321390.0 6 459812.0 7 415180.0 8 374417.0 9 473397.0 10 585756.0 11 525111.0 12 416231.0 13 342474.0 14 311638.0 15 303336.0 16 304599.0 17 306551.0 18 305777.0 19 305688.0 20 310290.0 21 326612.0 22 359893.0 23 415739.0 24 488499.0 25 574640.0 26 679681.0 27 800739.0 28 941671.0 29 1109864.0 30 1318610.0 31 1599368.0 32 1921653.0 33 2401576.0 34 3105393.0 35 4202481.0 36 6100707.0 37 1.0413872E7 38 1.4567475E7 39 4121203.0 40 9520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.391708034919873 16.883983946056773 14.763526897917323 42.96078112110603 2 16.107533324619514 20.942378929628788 48.49025490024786 14.459832845503842 3 20.39588248402001 24.988339587315107 33.86443161089864 20.75134631776624 4 22.08483173061052 31.858449794443374 28.57880238420637 17.477916090739736 5 20.06374080996085 35.04648164689155 30.25899020303115 14.630787340116447 6 15.626191975103614 38.01578136047798 30.818908417554276 15.539118246864133 7 13.0389474560527 20.448650979117886 49.308254664648096 17.204146900181318 8 14.127974906039341 22.416283646633335 41.59321508031394 21.862526367013384 9 15.602928584002804 24.366787659805667 40.41691279162274 19.613370964568787 10-11 19.016332028952792 35.34143970112672 29.384090668065603 16.258137601854887 12-13 17.8361187760066 27.58284690875876 36.411451006295266 18.169583308939373 14-15 18.882053900986975 28.889662691870477 35.23957480214452 16.988708604998028 16-17 19.505836325687063 28.194957365911367 33.92182046915789 18.37738583924368 18-19 19.201141573043014 29.234973403612297 33.50136574207786 18.062519281266827 20-21 19.626745510834525 28.417696415268612 33.02625637324785 18.929301700649017 22-23 19.154298204190013 30.688799822248736 32.994422197605935 17.162479775955315 24-25 18.560252516388275 30.376278144323315 32.48820977136236 18.575259567926057 26-27 18.983800036943546 30.754190286331333 31.75778983354811 18.504219843177008 28-29 18.82897350096206 30.556604512902254 32.069964341372525 18.544457644763163 30-31 18.152195692422097 30.53843827618763 32.440644166357025 18.868721865033248 32-33 18.28721775745571 30.37598989335705 32.54830314922487 18.78848919996236 34-35 18.971132756520646 31.235232573179992 31.0037018327321 18.78993283756726 36-37 19.486022440268354 30.90428044723873 31.474182500687665 18.135514611805252 38-39 18.79844804609934 30.46919685674785 32.01086806606977 18.721487031083043 40-41 19.110514433263987 31.025317973713623 30.935208913607266 18.928958679415125 42-43 18.94991167083663 31.035644904346206 31.37506480803543 18.639378616781737 44-45 19.336766249011976 30.532192350205467 31.193569940565997 18.937471460216557 46-47 19.121254200770846 30.154421695625306 31.791233000381304 18.933091103222548 48-49 19.000675939337054 29.810075466780756 31.611715857316135 19.577532736566056 50-51 18.316076476879882 30.50282964925418 32.58968901925338 18.59140485461256 52-53 19.68099570515059 30.313145412981545 31.454513865111167 18.551345016756695 54-55 19.37090550103816 30.344213912028945 31.059700826952973 19.225179759979923 56-57 19.914730399623576 30.383180130487148 30.40200763673576 19.300081833153516 58-59 18.742151487643675 31.428814195302724 30.90713244626988 18.921901870783724 60-61 19.18613032509914 30.95776569098146 30.12630895278684 19.72979503113256 62-63 19.249164193566706 31.1446825076354 30.700555563679593 18.905597735118295 64-65 19.911550610405733 30.80653254596769 30.304291568136225 18.977625275490354 66-67 19.42406640569999 31.43522975044541 29.99535404076874 19.14534980308586 68-69 19.313751957287874 31.375172512039974 30.668064686315276 18.643010844356876 70-71 19.535613345475163 31.36803243145243 30.10871848444564 18.987635738626768 72-73 19.338259593423253 31.241973793365545 30.256981470122053 19.162785143089152 74-75 18.936280970802006 30.526299320683986 32.21209274943169 18.325326959082325 76-77 18.779185202810837 31.18034494760514 31.261315506807946 18.779154342776078 78-79 19.35584554240363 31.263540805243217 30.673544168651794 18.707069483701353 80-81 19.131942184210573 31.04188498307961 30.873107710773056 18.953065121936756 82-83 19.73196400872548 31.433493097164256 30.085989234444078 18.74855365966619 84-85 19.366045841625045 30.92381964849403 30.421244754933596 19.288889754947323 86-87 19.043845582852367 31.80187981560747 30.340614961341068 18.813659640199095 88-89 18.74612382070608 31.31601616955883 30.97269517277806 18.96516483695703 90-91 19.058848148865497 31.727418798600876 30.629547790137007 18.58418526239662 92-93 19.365192167725464 30.733711049163837 30.49351575927912 19.407581023831575 94-95 19.0683431271306 31.154716279486028 30.42073042066716 19.356210172716214 96-97 19.482917981866823 31.22111511156503 29.992531221307217 19.30343568526093 98-99 19.489625389083585 31.033339817738337 30.238014945190837 19.23901984798724 100-101 20.348761629347536 31.99018708473228 28.925120594069853 18.73593069185033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24098.0 1 24781.0 2 21210.0 3 18131.0 4 21456.0 5 26475.0 6 30171.5 7 33017.0 8 37529.0 9 42649.0 10 48430.5 11 55567.5 12 63981.0 13 72778.5 14 82296.5 15 92795.0 16 104398.0 17 117890.0 18 135099.5 19 156180.0 20 187484.5 21 229351.0 22 277386.0 23 332835.5 24 413485.0 25 549000.5 26 801713.5 27 1187397.0 28 1407466.0 29 1491990.5 30 1765663.0 31 2109802.5 32 2358424.0 33 2647645.0 34 3092700.0 35 3657452.5 36 4006811.5 37 4259602.5 38 4156913.0 39 3219394.0 40 2701031.0 41 2595767.5 42 2207476.5 43 1958210.0 44 1833040.5 45 1709142.0 46 1526876.0 47 1375180.0 48 1300135.0 49 1232248.5 50 1111405.0 51 954736.0 52 826367.5 53 725044.0 54 642740.0 55 557731.5 56 480751.5 57 401865.0 58 326399.5 59 256952.0 60 190556.5 61 136829.0 62 99604.5 63 72849.5 64 51236.0 65 35513.5 66 24683.5 67 18377.0 68 14016.0 69 10500.0 70 9754.5 71 10827.5 72 10835.5 73 12543.5 74 12957.0 75 9103.5 76 6753.5 77 6288.0 78 5373.5 79 3797.0 80 2789.0 81 2075.0 82 1602.5 83 1360.5 84 1247.0 85 1149.5 86 893.5 87 590.5 88 383.0 89 275.5 90 193.0 91 128.0 92 76.5 93 40.0 94 21.0 95 11.0 96 5.5 97 5.0 98 5.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05199007820014866 2 0.005449826319758047 3 0.005736740470755782 4 0.00281052464041867 5 0.022592175567273238 6 0.10264739389243688 7 0.10959349293514009 8 0.11710879263224205 9 0.12468733684649941 10-11 0.13173370158713194 12-13 0.1415813356729897 14-15 0.14512071480989452 16-17 0.15167886272907125 18-19 0.15190253236291357 20-21 0.15828328712421535 22-23 0.12856916190556286 24-25 0.14117410268447678 26-27 0.1489439229993993 28-29 0.15546042209020539 30-31 0.16594435772061453 32-33 0.16956317814126337 34-35 0.1650134292790708 36-37 0.1196902487166222 38-39 0.18058469216749357 40-41 0.19118123388942335 42-43 0.18260466034346956 44-45 0.2000524343323694 46-47 0.19380742390057196 48-49 0.1976946478818316 50-51 0.19396322136966213 52-53 0.18907796805670607 54-55 0.1881269864755765 56-57 0.19621919957307174 58-59 0.19620685917948044 60-61 0.20346918080796075 62-63 0.21031115777973736 64-65 0.37225954180056897 66-67 0.17998849690211388 68-69 0.3381576353856097 70-71 0.011140290314546452 72-73 0.012434489092434085 74-75 0.02493607907502086 76-77 0.029328487918924354 78-79 0.019365162643148182 80-81 0.02240552711420482 82-83 0.04052276745543275 84-85 0.02010867135702403 86-87 0.021026488130377 88-89 0.02596573066529499 90-91 0.008868115344548263 92-93 0.013622251975596748 94-95 0.03174411996442141 96-97 0.030803936227684212 98-99 0.00858274374274944 100-101 0.017174742780692354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.4827754E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.106645061396613 #Duplication Level Percentage of deduplicated Percentage of total 1 76.20725196349898 17.60893922224991 2 8.805328829347161 4.069232158172156 3 3.8420673576982103 2.6633186020893147 4 2.2486130126506385 2.0783161105502415 5 1.5222198288599398 1.7586696645443263 6 1.0700767988656088 1.483553086789387 7 0.823301410882801 1.3316613435871156 8 0.6156530035827147 1.1380540347814823 9 0.5273472470275116 1.0966703095052412 >10 3.6650432158482196 16.698437833801997 >50 0.38890911088419666 6.2297290404995955 >100 0.23677212434390876 10.36977084135685 >500 0.021996361154797823 3.5228688397089827 >1k 0.01998989733132405 9.774363225609047 >5k 0.0029604554316979047 4.722365540135189 >10k+ 0.002469382592033989 15.45405014661902 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 277213 0.42761469107814537 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 270902 0.4178796630838082 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 221340 0.3414278396873043 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 166392 0.25666784630545736 No Hit CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA 161191 0.24864504792191322 No Hit GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT 151734 0.2340571601477972 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 110759 0.17085120672235538 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 110357 0.1702311019443925 No Hit CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT 100063 0.1543521004907867 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 97631 0.15060062083903136 No Hit CAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCT 82488 0.1272417983198986 No Hit CTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAAC 80821 0.12467036880531139 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA 79556 0.122719044068687 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 77241 0.11914804267320445 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 76604 0.11816543883349714 No Hit CCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCC 71788 0.1107365218915343 No Hit CCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT 70930 0.10941301467886733 No Hit CAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCAT 69300 0.10689865948463985 No Hit CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC 68801 0.1061289274343825 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 68297 0.10535148263813059 No Hit CAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCG 66101 0.10196404459731861 No Hit GGAAAAGTCAGATTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 65762 0.10144112041888727 No Hit CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 65434 0.10093516428164392 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.023223078189628474 1.5425491989125522E-6 0.0 6.170196795650209E-6 0.0 2 0.02331100349396649 4.627647596737656E-6 0.0 7.71274599456276E-6 0.0 3 0.02348685410264252 4.627647596737656E-6 0.0 9.255295193475312E-6 0.0 4 0.024216479873728155 4.627647596737656E-6 0.0 1.542549198912552E-5 0.0 5 0.024494138729532416 4.627647596737656E-6 0.0 2.005313958586318E-5 0.0 6 0.02479185072492254 4.627647596737656E-6 0.0 2.6223336381513386E-5 0.0 7 0.024978499177990957 4.627647596737656E-6 0.0 3.54786315749887E-5 0.0 8 0.02573434828545811 4.627647596737656E-6 0.0 3.702118077390125E-5 0.0 9 0.02601817733805802 4.627647596737656E-6 0.0 4.3191377569551464E-5 0.0 10-11 0.026844983708675146 4.627647596737656E-6 0.0 4.3191377569551464E-5 0.0 12-13 0.028153836703952446 4.627647596737656E-6 0.0 4.704775056683284E-5 0.0 14-15 0.02955292882736613 4.627647596737656E-6 0.0 5.6303045760308153E-5 0.0 16-17 0.03120499901940147 4.627647596737656E-6 0.0 6.710089015269602E-5 0.0 18-19 0.03318640346540465 5.398922196193932E-6 0.0 8.869657893747175E-5 0.0 20-21 0.03535445636447624 6.170196795650209E-6 0.0 9.872314873040334E-5 0.0 22-23 0.03757109956331357 6.170196795650209E-6 0.0 1.0180824712822844E-4 0.0 24-25 0.03933963221986682 6.170196795650209E-6 0.0 1.1337736612007258E-4 0.0 26-27 0.040921516423351637 6.170196795650209E-6 0.0 1.318879565070232E-4 0.0 28-29 0.042668453391120104 6.170196795650209E-6 0.0 1.4037197710104225E-4 0.0 30-31 0.04460975155795155 6.170196795650209E-6 0.0 1.4962727229451755E-4 0.0 32-33 0.04665054414811286 7.71274599456276E-6 0.0 1.5271237069234268E-4 0.0 34-35 0.048867958621549655 1.1569118991844143E-5 0.0 1.5888256748799288E-4 0.0 36-37 0.051236542916479874 1.2340393591300418E-5 0.0 1.7199423567874957E-4 0.0 38-39 0.053784062918483956 1.2340393591300418E-5 0.0 1.743080594771184E-4 0.0 40-41 0.05658995991130589 1.2340393591300418E-5 0.0 1.773931578749435E-4 0.0 42-43 0.05977840910545813 1.2340393591300418E-5 0.0 2.0053139585863178E-4 0.0 44-45 0.06337177746432493 1.2340393591300418E-5 0.0 2.1518561324830105E-4 0.0 46-47 0.06750195294441329 1.388294279021297E-5 0.0 2.3446747823470794E-4 0.0 48-49 0.0718496278615483 1.388294279021297E-5 0.0 2.4680787182600834E-4 0.0 50-51 0.07679041294566522 1.4654217389669246E-5 0.0 2.5297806862165854E-4 0.0 52-53 0.0827053178488954 1.542549198912552E-5 0.0 2.5914826541730874E-4 1.5425491989125522E-6 54-55 0.08926655086646994 1.6968041188038075E-5 0.0 2.676322860113278E-4 1.5425491989125522E-6 56-57 0.09664610623406758 1.8510590386950625E-5 0.0 2.745737574064343E-4 1.5425491989125522E-6 58-59 0.10533374332234308 1.8510590386950625E-5 0.0 2.838290525999096E-4 1.5425491989125522E-6 60-61 0.1147055009803363 1.8510590386950625E-5 0.0 2.884567001966473E-4 1.5425491989125522E-6 62-63 0.12552339851231004 2.1595688784775732E-5 7.712745994562761E-7 2.9848326998957887E-4 1.5425491989125522E-6 64-65 0.13818387106238478 2.1595688784775732E-5 1.5425491989125522E-6 3.0850983978251045E-4 1.5425491989125522E-6 66-67 0.15397726103545095 2.1595688784775732E-5 1.5425491989125522E-6 3.154513111776169E-4 1.5425491989125522E-6 68-69 0.17176825222110886 2.1595688784775732E-5 1.5425491989125522E-6 3.2085023337381086E-4 2.3138237983688285E-6 70-71 0.19166405178868298 2.3138237983688282E-5 1.5425491989125522E-6 3.2856297936837363E-4 3.0850983978251044E-6 72-73 0.21392535055278947 2.6223336381513386E-5 1.5425491989125522E-6 3.3396190156456755E-4 3.0850983978251044E-6 74-75 0.24082432348342656 2.6223336381513386E-5 1.5425491989125522E-6 3.3704699996239265E-4 3.85637299728138E-6 76-77 0.2729949583013473 2.6223336381513386E-5 1.5425491989125522E-6 3.5941396334662464E-4 4.627647596737656E-6 78-79 0.30937752370689875 2.6223336381513386E-5 1.5425491989125522E-6 3.709830823384688E-4 4.627647596737656E-6 80-81 0.3496126057367343 2.8537160179882214E-5 1.5425491989125522E-6 3.840947505292255E-4 4.627647596737656E-6 82-83 0.3996505879256591 3.239353317716359E-5 1.5425491989125522E-6 3.956638695210696E-4 4.627647596737656E-6 84-85 0.4582057555163796 3.4707356975532425E-5 1.5425491989125522E-6 4.0260534091617615E-4 4.627647596737656E-6 86-87 0.5279660004880009 3.54786315749887E-5 1.5425491989125522E-6 4.1108936151019516E-4 4.627647596737656E-6 88-89 0.6070987743922147 3.779245537335753E-5 1.5425491989125522E-6 4.164882837063891E-4 6.170196795650209E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 45620 0.0 71.761444 1 GCGTGTT 63970 0.0 51.17491 2 CGTGTTT 87150 0.0 38.03798 3 CCTTTAG 81150 0.0 33.02598 5 ACTCGTC 93085 0.0 32.684673 42-43 TCGTCTA 94980 0.0 31.957357 44-45 TTAGGCA 86490 0.0 30.689993 8 TAGGCAT 88050 0.0 30.049683 9 GTCTATG 103280 0.0 29.455288 46-47 CTAACGA 99875 0.0 29.424833 94-95 CCCGTAT 8715 0.0 29.31501 1 GTTTTTT 696445 0.0 29.07337 1 TCCTTTA 91370 0.0 28.318563 4 ATGTGGC 108735 0.0 27.8148 50-51 CCCGAAA 104115 0.0 27.6717 1 CTATGTG 112045 0.0 27.097382 48-49 GCCTAAC 109525 0.0 26.88064 92-93 GTGGCAA 113435 0.0 26.788567 52-53 CTTTAGG 100625 0.0 26.686281 6 GAGGTGA 109965 0.0 26.656391 82-83 >>END_MODULE