FastQCFastQC Report
Fri 27 May 2016
SRR1233036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26865909
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT843250.31387361581549317No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA777070.28924016678534864No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA666710.24816208526575448No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC552540.2056658496088854No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA540340.20112477861813646No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT363550.13532019333498077No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328430.1222478643845626No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT321260.11957905463016345No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA304480.11333322092321536No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC288300.10731071857646804No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC286110.10649555911173526No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT271810.10117282836028367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT209300.078.3728261
GCGTGTT283550.057.7287142
CGTGTTT422850.039.7456133
CCTTTAG245350.037.7645535
TAGGCAT255650.035.8526089
GTTTTTT3281650.035.3124921
TTAGGCA266100.034.6764768
TTTAGGC277800.034.001597
CCCGAAA270900.032.8648381
ACTCGTC244400.032.38532642-43
TCGTCTA244100.032.32478344-45
CTAACGA237400.032.1302694-95
CTTTAGG299500.031.3147586
GCCTAAC270750.028.36518392-93
CAAAACG158150.027.7480169
TCGTACC25850.027.2198472
CGCGAGT4950.026.02481
CCGAAAC342450.025.9198042
TCCTTTA370150.025.8929214
GTCTATG313300.025.41795546-47
Produced by FastQC (version 0.11.5)