FastQCFastQC Report
Fri 27 May 2016
SRR1233036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26865909
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT795470.29608899516483883No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA672010.2501348456141945No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA622990.23188867348579198No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA516910.19240368900229654No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC384380.14307351372328403No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT314790.11717079812933187No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC294520.10962592034388266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT207800.079.360641
GCGTGTT280000.059.0601352
CGTGTTT407450.041.519283
CCCGAAA245700.034.2069241
TCGTCTA244400.033.03060544-45
ACTCGTC245950.032.93850342-43
CCTTTAG231700.032.471525
CTAACGA243400.032.22988594-95
TAGGCAT233350.031.9013379
TTAGGCA236550.031.4490668
GTTTTTT3126850.031.3207991
TTTAGGC255550.030.0768747
GCCTAAC279850.028.33798492-93
TCGTACC26450.027.9945342
CTTTAGG277850.027.3857696
CCGAAAC314850.026.9096982
CCCGTAT28800.026.874541
GTCTATG309550.026.33145946-47
CCAAACG349800.026.1531288
CGCGCGT11300.025.2126621
Produced by FastQC (version 0.11.5)