##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233036_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26865909 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.534744646086608 31.0 30.0 34.0 25.0 34.0 2 29.94714796361441 31.0 30.0 34.0 26.0 34.0 3 28.814066704387333 31.0 28.0 34.0 16.0 34.0 4 31.844824383198798 35.0 32.0 37.0 19.0 37.0 5 32.557570897750004 35.0 33.0 37.0 22.0 37.0 6 32.935399282414004 35.0 33.0 37.0 26.0 37.0 7 33.21642081792207 37.0 35.0 37.0 28.0 37.0 8 33.23586765666481 37.0 35.0 37.0 27.0 37.0 9 34.718938860397394 39.0 35.0 39.0 27.0 39.0 10-11 34.599021719309775 39.0 35.0 39.0 27.0 39.0 12-13 34.50381312242217 39.0 35.0 39.0 27.0 39.0 14-15 35.605785365386296 40.0 36.0 41.0 27.0 41.0 16-17 35.45312872160774 40.0 36.0 41.0 26.0 41.0 18-19 35.221630598838104 39.5 35.5 41.0 25.0 41.0 20-21 34.97509531503289 39.0 35.0 41.0 23.5 41.0 22-23 34.757417792936025 39.0 35.0 41.0 21.0 41.0 24-25 34.46509753308551 39.0 34.0 41.0 18.0 41.0 26-27 34.07380595236886 39.0 34.0 41.0 12.0 41.0 28-29 33.80734586348818 39.0 34.0 41.0 9.0 41.0 30-31 33.515387437663094 39.0 33.0 41.0 3.5 41.0 32-33 33.242102379636584 39.0 33.0 41.0 2.0 41.0 34-35 33.041910325833385 39.0 33.0 41.0 2.0 41.0 36-37 32.94725853124865 38.5 33.0 40.5 2.0 41.0 38-39 32.95477889841732 39.0 33.0 41.0 2.0 41.0 40-41 32.88418783820045 39.0 33.0 41.0 2.0 41.0 42-43 32.786523601341756 39.0 33.0 41.0 2.0 41.0 44-45 32.69665872835347 39.0 33.0 41.0 2.0 41.0 46-47 32.5547991880714 38.5 32.0 41.0 2.0 41.0 48-49 32.44015424901499 38.5 32.0 41.0 2.0 41.0 50-51 32.33798834053968 38.0 32.0 41.0 2.0 41.0 52-53 32.16616990327779 38.0 32.0 40.5 2.0 41.0 54-55 32.00917113952853 38.0 31.0 40.0 2.0 41.0 56-57 31.85162087759621 38.0 31.0 40.0 2.0 41.0 58-59 31.7138394051733 38.0 31.0 40.0 2.0 41.0 60-61 31.65243567600858 38.0 31.0 40.0 2.0 41.0 62-63 31.47032828853846 38.0 31.0 40.0 2.0 41.0 64-65 31.039133218980233 37.0 30.0 40.0 2.0 41.0 66-67 30.87187232339691 37.0 30.0 40.0 2.0 41.0 68-69 30.509103358460717 36.0 29.0 39.5 2.0 41.0 70-71 30.224300618304035 36.0 29.0 39.0 2.0 41.0 72-73 29.94575631146521 35.5 29.0 39.0 2.0 41.0 74-75 29.607789224626643 35.0 29.0 38.5 2.0 40.0 76-77 28.48829693422992 34.0 27.0 37.0 2.0 39.0 78-79 28.912552112046534 35.0 28.5 37.0 2.0 39.0 80-81 28.729934449640247 35.0 29.0 37.0 2.0 39.0 82-83 28.424858153878212 35.0 29.0 36.0 2.0 39.0 84-85 28.118050835354204 34.5 28.0 36.0 2.0 37.0 86-87 27.77171139081875 34.0 27.0 35.5 2.0 37.0 88-89 27.50409500382064 34.0 26.5 35.0 2.0 36.5 90-91 27.239735886100114 34.0 26.0 35.0 2.0 36.0 92-93 26.982130271490163 34.0 26.0 35.0 2.0 36.0 94-95 26.713997244612123 34.0 25.5 35.0 2.0 36.0 96-97 26.462644089206137 34.0 25.0 35.0 2.0 35.5 98-99 26.14202294439395 34.0 24.0 35.0 2.0 35.0 100-101 25.24952087048311 33.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6987402128491809 1101 2 1.5925336307820217 1101 3 2.7384952673621825 1101 4 3.047331265323038 1101 5 2.0315048494756667 1101 6 1.6230267300667833 1101 7 1.4306544511029955 1101 8 1.3861405789957502 1101 9 1.4327662042395914 1101 10-11 1.5001238004598605 1101 12-13 1.4650170259425437 1101 14-15 1.617324256117044 1101 16-17 1.6714355281152038 1101 18-19 1.6527436584422972 1101 20-21 1.6230808752980863 1101 22-23 1.5811140254212788 1101 24-25 1.7291888753741702 1101 26-27 1.5849624931577537 1101 28-29 1.7259162586407868 1101 30-31 1.7547932192179516 1101 32-33 1.7583884591231538 1101 34-35 1.8019856526696074 1101 36-37 1.7336145415178024 1101 38-39 1.6934390910067947 1101 40-41 1.7570570745462106 1101 42-43 1.7834076100548941 1101 44-45 1.8039774699515618 1101 46-47 1.73122901581754 1101 48-49 1.6887365422244045 1101 50-51 1.6717450439163102 1101 52-53 1.6916965466647937 1101 54-55 1.6909102585109608 1101 56-57 1.6568359542969588 1101 58-59 1.6585237214102122 1101 60-61 1.6129061207818687 1101 62-63 1.7414989858009164 1101 64-65 1.6724390715939528 1101 66-67 1.754067242861101 1101 68-69 1.685157834296934 1101 70-71 1.7389560261138826 1101 72-73 1.7564826522310746 1101 74-75 1.7201971855939 1101 76-77 1.5905321136758452 1101 78-79 1.7255426003027488 1101 80-81 1.7254874185287576 1101 82-83 1.7510853727232956 1101 84-85 1.796764719233007 1101 86-87 1.7496683208380652 1101 88-89 1.6938182390856227 1101 90-91 1.7678456899625807 1101 92-93 1.8329066684174435 1101 94-95 1.861704837690727 1101 96-97 1.8777463110934498 1101 98-99 1.9472000921262875 1101 100-101 1.9626728555277353 1102 1 1.6491212650881835 1102 2 1.518237721927207 1102 3 2.6806876355304716 1102 4 3.016988143356066 1102 5 2.020751052914079 1102 6 1.6039354519864801 1102 7 1.3793578386235765 1102 8 1.3355475557793852 1102 9 1.4098671959304028 1102 10-11 1.4673737383961267 1102 12-13 1.5047396268540751 1102 14-15 1.6669294374633097 1102 16-17 1.6084799552160476 1102 18-19 1.5868775314709254 1102 20-21 1.599409843270088 1102 22-23 1.6496273950663678 1102 24-25 1.6991679475962727 1102 26-27 1.6627814113289077 1102 28-29 1.5873116125412068 1102 30-31 1.6286062414757936 1102 32-33 1.631962442345305 1102 34-35 1.6694615471295933 1102 36-37 1.6833903751581545 1102 38-39 1.7146644549561572 1102 40-41 1.7075485496894771 1102 42-43 1.6802906958354598 1102 44-45 1.7157995102536816 1102 46-47 1.7442533872137034 1102 48-49 1.7240085089689856 1102 50-51 1.666882672012889 1102 52-53 1.7113512846300125 1102 54-55 1.7259139833952943 1102 56-57 1.6929926826842987 1102 58-59 1.6256737777341073 1102 60-61 1.6478846013485757 1102 62-63 1.6755573644473714 1102 64-65 1.676696101195379 1102 66-67 1.6714908797280295 1102 68-69 1.640628793064277 1102 70-71 1.5903905748879197 1102 72-73 1.6209999073627834 1102 74-75 1.6537748353830963 1102 76-77 1.5870077171148793 1102 78-79 1.6446436809892795 1102 80-81 1.6528560971819743 1102 82-83 1.6523915244975385 1102 84-85 1.6905644903888977 1102 86-87 1.6165779455800937 1102 88-89 1.507874215059747 1102 90-91 1.567664303752835 1102 92-93 1.6409817557095892 1102 94-95 1.6814627778194406 1102 96-97 1.712035594397289 1102 98-99 1.74572477779963 1102 100-101 1.6961164179634274 1103 1 1.6626777709267984 1103 2 1.5297533676671584 1103 3 2.687285807773634 1103 4 3.034867954753807 1103 5 2.067592107723577 1103 6 1.690196326107099 1103 7 1.4407513914953967 1103 8 1.3380863916532988 1103 9 1.4799653463727225 1103 10-11 1.4976525161626455 1103 12-13 1.5279127119535119 1103 14-15 1.6539124347563217 1103 16-17 1.6842685533414326 1103 18-19 1.6508737771701334 1103 20-21 1.702423494232697 1103 22-23 1.749275066020246 1103 24-25 1.7515885410000962 1103 26-27 1.7892208704358339 1103 28-29 1.7435332158556207 1103 30-31 1.7593849364858727 1103 32-33 1.735953830035264 1103 34-35 1.7434927639334674 1103 36-37 1.7132040275524574 1103 38-39 1.7951637439340686 1103 40-41 1.7307816006711363 1103 42-43 1.723783544832827 1103 44-45 1.819846355877445 1103 46-47 1.829916256556352 1103 48-49 1.853892284704017 1103 50-51 1.8191024780048863 1103 52-53 1.7829334720270467 1103 54-55 1.7868239113592281 1103 56-57 1.829820488599502 1103 58-59 1.831134494942873 1103 60-61 1.8568473165885315 1103 62-63 1.903880192882525 1103 64-65 1.8432252268535017 1103 66-67 1.8187738110978806 1103 68-69 1.7902778685999827 1103 70-71 1.7881780385558912 1103 72-73 1.7777394027719176 1103 74-75 1.8154903466085806 1103 76-77 1.713976883886577 1103 78-79 1.7585133123142107 1103 80-81 1.7868837892447083 1103 82-83 1.791309384769523 1103 84-85 1.7905225657907522 1103 86-87 1.7824945257845357 1103 88-89 1.5504715346837834 1103 90-91 1.5926332877119656 1103 92-93 1.6156895010076013 1103 94-95 1.6261936762116704 1103 96-97 1.5793257281807236 1103 98-99 1.5916385455865694 1103 100-101 1.5921960321557123 1104 1 1.495624679069639 1104 2 1.3941507164568883 1104 3 2.5388576270911543 1104 4 2.928937230813844 1104 5 1.8721075011766786 1104 6 1.5298417175236807 1104 7 1.3113479226261688 1104 8 1.1983240539327369 1104 9 1.3156233871113727 1104 10-11 1.3138259219091424 1104 12-13 1.3565415175054696 1104 14-15 1.4766096882364366 1104 16-17 1.4764174538097805 1104 18-19 1.4405382499969974 1104 20-21 1.4623772737102954 1104 22-23 1.4171846164654767 1104 24-25 1.4899114843999612 1104 26-27 1.4896237037251154 1104 28-29 1.4499958745298045 1104 30-31 1.4443495772817272 1104 32-33 1.4233454490462023 1104 34-35 1.4302206606117593 1104 36-37 1.4372653948937781 1104 38-39 1.4870064336904747 1104 40-41 1.4331221955798767 1104 42-43 1.443409337191504 1104 44-45 1.432487601587134 1104 46-47 1.4335103973922045 1104 48-49 1.3951379672427393 1104 50-51 1.3796234035875727 1104 52-53 1.4276686776650038 1104 54-55 1.4906403372059103 1104 56-57 1.465345070658259 1104 58-59 1.4696268812849667 1104 60-61 1.4463295117329942 1104 62-63 1.4753151255093826 1104 64-65 1.4536240986472144 1104 66-67 1.5195126364331877 1104 68-69 1.474684180654819 1104 70-71 1.4291582612042504 1104 72-73 1.3866671402473685 1104 74-75 1.4530929048916938 1104 76-77 1.327284718265691 1104 78-79 1.382838567091067 1104 80-81 1.4130473776573034 1104 82-83 1.4429127180257026 1104 84-85 1.4067787588830178 1104 86-87 1.38515615527405 1104 88-89 1.3606764863467369 1104 90-91 1.447372613667575 1104 92-93 1.467283150953051 1104 94-95 1.4530178815216317 1104 96-97 1.3774730133106274 1104 98-99 1.484357921638967 1104 100-101 1.5288155942243549 1105 1 1.3231584751024599 1105 2 1.2011210716790899 1105 3 2.3478894463269953 1105 4 2.7253429819021093 1105 5 1.6770692021362734 1105 6 1.2357572668016132 1105 7 0.9875311224329266 1105 8 0.877689635831949 1105 9 0.9270110032416952 1105 10-11 0.9777276840045914 1105 12-13 1.0608643624343657 1105 14-15 1.211585724013446 1105 16-17 1.177664724203936 1105 18-19 1.1687831893740324 1105 20-21 1.1825742083889637 1105 22-23 1.184966088898058 1105 24-25 1.2593068248344181 1105 26-27 1.2021543157800991 1105 28-29 1.1662333822573885 1105 30-31 1.1615876921260693 1105 32-33 1.1447317714155432 1105 34-35 1.1483896170542849 1105 36-37 1.2176316127076845 1105 38-39 1.246615779536988 1105 40-41 1.2243483583359804 1105 42-43 1.2304534503413365 1105 44-45 1.1867473242838784 1105 46-47 1.260066742664712 1105 48-49 1.2318746642183385 1105 50-51 1.2220082283404992 1105 52-53 1.2323713734592587 1105 54-55 1.1099659364023964 1105 56-57 1.211847507679991 1105 58-59 1.2517862409708584 1105 60-61 1.2348436816851027 1105 62-63 1.2087050798958359 1105 64-65 1.1835014377743498 1105 66-67 1.2833948791809426 1105 68-69 1.285676284929604 1105 70-71 1.2632932365901013 1105 72-73 1.234841295788538 1105 74-75 1.3044677009755468 1105 76-77 1.2722726678014666 1105 78-79 1.3737776105646553 1105 80-81 1.4034758672045768 1105 82-83 1.3746071622878873 1105 84-85 1.390668358992862 1105 86-87 1.345412640790201 1105 88-89 1.354909810492213 1105 90-91 1.384821184099529 1105 92-93 1.426266423095683 1105 94-95 1.4063975130371347 1105 96-97 1.3900769776377437 1105 98-99 1.4568078350142386 1105 100-101 1.4221464641867954 1106 1 1.25575002832133 1106 2 1.1323716569275852 1106 3 2.3427645615411663 1106 4 2.738000298798262 1106 5 1.7676392891761523 1106 6 1.163304013897033 1106 7 1.0154891348093216 1106 8 0.861679874617451 1106 9 0.9026058193901463 1106 10-11 0.9828743544666452 1106 12-13 1.0330497497957012 1106 14-15 1.086175683367074 1106 16-17 1.0980951189900878 1106 18-19 1.0352766887068015 1106 20-21 0.9887381221126503 1106 22-23 0.9489270395518687 1106 24-25 1.0266334724750479 1106 26-27 1.048393819203497 1106 28-29 1.0911310906450282 1106 30-31 1.0381379417062533 1106 32-33 0.9610091710528152 1106 34-35 1.0800588785405623 1106 36-37 1.0846741169354672 1106 38-39 1.0705304265629891 1106 40-41 1.0632075737954594 1106 42-43 1.0503404933094558 1106 44-45 1.0930703334493401 1106 46-47 1.0194252538854514 1106 48-49 1.1181276970827625 1106 50-51 1.1763259755314834 1106 52-53 1.162170619951631 1106 54-55 1.2411442122427516 1106 56-57 1.3116421452532236 1106 58-59 1.2884905749240971 1106 60-61 1.263957552353741 1106 62-63 1.2835637335926435 1106 64-65 1.256167244530797 1106 66-67 1.3047820056171808 1106 68-69 1.2736823267240744 1106 70-71 1.265008123432537 1106 72-73 1.2836979667942217 1106 74-75 1.3013672703849757 1106 76-77 1.1556682889656464 1106 78-79 1.2533818020504874 1106 80-81 1.2957692803193837 1106 82-83 1.2528566192680728 1106 84-85 1.29248268665226 1106 86-87 1.2861666056913954 1106 88-89 1.2322511526096633 1106 90-91 1.2659636375146661 1106 92-93 1.2532812828067108 1106 94-95 1.242610455346302 1106 96-97 1.2313318591488631 1106 98-99 1.2465445807751792 1106 100-101 1.2419972920118667 1107 1 1.1145554921502878 1107 2 1.0297912935326181 1107 3 2.1773999046227175 1107 4 2.577549694721874 1107 5 1.5326234213652228 1107 6 1.0956989219789293 1107 7 0.9297810082983489 1107 8 0.8682513432882288 1107 9 0.8801506219318398 1107 10-11 0.8789265311215502 1107 12-13 0.8764059001671356 1107 14-15 0.9090738949420967 1107 16-17 0.8897015141763944 1107 18-19 0.8995703778592201 1107 20-21 0.9334196663536787 1107 22-23 0.9100172909744302 1107 24-25 0.8660291242168299 1107 26-27 0.7776919578291697 1107 28-29 0.8613623298575561 1107 30-31 0.8772529920515879 1107 32-33 0.8720175091943574 1107 34-35 0.8874819271832735 1107 36-37 0.8715926346922558 1107 38-39 0.8586543813326486 1107 40-41 0.8600178769499109 1107 42-43 0.8849972853065964 1107 44-45 0.8638135689670321 1107 46-47 0.7774108983406123 1107 48-49 0.8351632489127425 1107 50-51 0.7830525647148932 1107 52-53 0.8041911680034701 1107 54-55 0.7569489133525451 1107 56-57 0.7692708151267382 1107 58-59 0.8562007666835854 1107 60-61 0.8374127963431768 1107 62-63 0.9233452355826302 1107 64-65 0.8350386861396579 1107 66-67 0.8625097440290865 1107 68-69 0.8934859647306901 1107 70-71 0.9203580035346306 1107 72-73 0.92883193037202 1107 74-75 0.9126615516655185 1107 76-77 0.8074861630711361 1107 78-79 0.8945990876193868 1107 80-81 0.9250161473927463 1107 82-83 0.9435061448130284 1107 84-85 0.9212416098839817 1107 86-87 0.9392490941782441 1107 88-89 0.8859543860336103 1107 90-91 0.8539896476271736 1107 92-93 0.8060539734759367 1107 94-95 0.8329926660111795 1107 96-97 0.8483853264267545 1107 98-99 0.9029792201622087 1107 100-101 0.8887732795364371 1108 1 1.176802855738046 1108 2 1.0547553109337287 1108 3 2.177747534423723 1108 4 2.619181627587807 1108 5 1.5319116980924932 1108 6 1.1093524202681309 1108 7 0.8737717878067954 1108 8 0.8632200549795854 1108 9 0.7892266689015273 1108 10-11 0.8204561421901602 1108 12-13 0.8716265983889926 1108 14-15 0.8863629394061832 1108 16-17 0.8846613798855216 1108 18-19 0.8518313690209709 1108 20-21 0.8600289789779154 1108 22-23 0.8919034253610434 1108 24-25 0.8840094049998797 1108 26-27 0.9807133835739421 1108 28-29 0.910567995353162 1108 30-31 0.9136518602816253 1108 32-33 0.8917766008284431 1108 34-35 0.843268825719619 1108 36-37 0.8529370195778014 1108 38-39 0.8775951040267032 1108 40-41 0.8880814738830054 1108 42-43 0.8933724687566027 1108 44-45 0.9153950236724171 1108 46-47 0.8867520320962754 1108 48-49 0.8817677510418562 1108 50-51 0.9330588488273293 1108 52-53 0.9062014913253478 1108 54-55 0.8767931173634551 1108 56-57 0.9594399597705419 1108 58-59 0.8212380932290415 1108 60-61 0.9288522596221078 1108 62-63 0.9381237750300215 1108 64-65 0.9275536921135625 1108 66-67 0.9063010330590124 1108 68-69 0.899135886729308 1108 70-71 0.7509092101482935 1108 72-73 0.7418117897892103 1108 74-75 0.7202556003327594 1108 76-77 0.618312127598255 1108 78-79 0.744114782833666 1108 80-81 0.7074001265812626 1108 82-83 0.7741088968818417 1108 84-85 0.7701908926221499 1108 86-87 0.7917420462465223 1108 88-89 0.8004846417730818 1108 90-91 0.8034105846127773 1108 92-93 0.8076210712413712 1108 94-95 0.7011280748715052 1108 96-97 0.5715728506665805 1108 98-99 0.6325978424879963 1108 100-101 0.5766239014550756 1109 1 0.7152503534527632 1109 2 0.6431414132533178 1109 3 0.07563273229293799 1109 4 1.4217698663463452 1109 5 0.8096047608033601 1109 6 0.6321648735854737 1109 7 0.5488264231397366 1109 8 0.4901503565900285 1109 9 0.5043979617131527 1109 10-11 0.4867672049483929 1109 12-13 0.4791279246866722 1109 14-15 0.4440747861083594 1109 16-17 0.5375089289263428 1109 18-19 0.505483151400469 1109 20-21 0.5053556655584899 1109 22-23 0.5447294948462869 1109 24-25 0.5679189217565508 1109 26-27 0.5965278668174889 1109 28-29 0.5172437791437545 1109 30-31 0.4374702677258995 1109 32-33 0.48686735914325396 1109 34-35 0.4458654788627143 1109 36-37 0.43105627623253184 1109 38-39 0.525877577219326 1109 40-41 0.5437955276357158 1109 42-43 0.5528286919554333 1109 44-45 0.5195227808610241 1109 46-47 0.5376651579196476 1109 48-49 0.567937537881555 1109 50-51 0.5169857924708481 1109 52-53 0.5328182276560938 1109 54-55 0.5265007314053705 1109 56-57 0.5096271103010608 1109 58-59 0.47375613599697175 1109 60-61 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-0.7146411371606547 2311 84-85 -0.7310243745299196 2311 86-87 -0.6883995453805234 2311 88-89 -0.6029273306842917 2311 90-91 -0.6233678968251368 2311 92-93 -0.639659497556071 2311 94-95 -0.6072263232224344 2311 96-97 -0.584419861783676 2311 98-99 -0.5872411329734994 2311 100-101 -0.5357245396041037 2312 1 0.31343685083032113 2312 2 0.43603192481749176 2312 3 1.6646835829739395 2312 4 1.6956695471761307 2312 5 0.692628957653838 2312 6 0.35047304869078033 2312 7 0.2143773493314285 2312 8 0.15250268076389517 2312 9 0.08875396746622499 2312 10-11 0.04665390370877276 2312 12-13 0.03484592456076996 2312 14-15 -0.21366256333708122 2312 16-17 -0.1742125391270406 2312 18-19 -0.6491107245703631 2312 20-21 -0.6495460834264648 2312 22-23 -0.3246150406697481 2312 24-25 -0.03854736074137577 2312 26-27 0.020093634461396448 2312 28-29 -0.06471348174476788 2312 30-31 -0.5589332742202728 2312 32-33 -0.6322651918675817 2312 34-35 -0.4541792845563535 2312 36-37 -0.3491641096623965 2312 38-39 -0.2521744296851409 2312 40-41 -0.775658406898593 2312 42-43 -0.44949422316950916 2312 44-45 -0.19166214299734463 2312 46-47 -0.37459764581442556 2312 48-49 -0.14276749381962617 2312 50-51 -0.10033079446212412 2312 52-53 -0.4964668285204681 2312 54-55 -0.21005546186516 2312 56-57 -0.13968860647264592 2312 58-59 -0.08819558535446248 2312 60-61 -0.09336324633516213 2312 62-63 -0.1222892608304349 2312 64-65 -0.03230165204895741 2312 66-67 -0.2808423467165646 2312 68-69 -0.11912780644245302 2312 70-71 -0.05743391896558592 2312 72-73 -0.06053361189662354 2312 74-75 -0.20688851985054768 2312 76-77 -0.4735182221355778 2312 78-79 -0.25700891504957113 2312 80-81 -0.2554526882108732 2312 82-83 -0.25434275441165255 2312 84-85 -0.26276620179762844 2312 86-87 -0.2515062361529168 2312 88-89 -0.2627429671513788 2312 90-91 -0.28389216060669753 2312 92-93 -0.3644485346740396 2312 94-95 -0.35670640525778907 2312 96-97 -0.4710762126408703 2312 98-99 -0.4769279584725794 2312 100-101 -0.5586109796395675 2313 1 -1.5216511480580444 2313 2 -0.994933528055757 2313 3 -3.8023559302475647 2313 4 -4.928782980415118 2313 5 -3.0098418919196384 2313 6 -2.7274234283870413 2313 7 -1.8193184542187488 2313 8 -1.449690429449852 2313 9 -1.4052328989889773 2313 10-11 -1.3348537922729662 2313 12-13 -1.3643951781082464 2313 14-15 -1.4611989504865193 2313 16-17 -1.6993036628665266 2313 18-19 -1.6158323006217117 2313 20-21 -1.5512146818991752 2313 22-23 -1.9828665712596987 2313 24-25 -1.652499775511707 2313 26-27 -2.134824903989962 2313 28-29 -2.2421427339621047 2313 30-31 -1.8992251526285742 2313 32-33 -1.9621721488346182 2313 34-35 -1.9131310747591073 2313 36-37 -2.1579966196039138 2313 38-39 -2.7369005557342945 2313 40-41 -2.5031228309011695 2313 42-43 -2.05812508779859 2313 44-45 -1.9211199519740418 2313 46-47 -1.7798531159664464 2313 48-49 -1.81558182685108 2313 50-51 -1.7875898209409797 2313 52-53 -2.154557210026624 2313 54-55 -1.9782786299955575 2313 56-57 -1.964015910766534 2313 58-59 -1.8827685640194858 2313 60-61 -1.9332863509424136 2313 62-63 -1.8419675639154853 2313 64-65 -1.799869064821344 2313 66-67 -1.9940759739670533 2313 68-69 -1.959813272175161 2313 70-71 -1.9106365659890656 2313 72-73 -1.9256709012263507 2313 74-75 -1.750867295567705 2313 76-77 -1.692113544733008 2313 78-79 -1.8159090253220853 2313 80-81 -1.803095369071272 2313 82-83 -1.8408150891012127 2313 84-85 -1.7960291073496073 2313 86-87 -1.8003225010380426 2313 88-89 -1.7196795608984452 2313 90-91 -1.7324774540140808 2313 92-93 -1.6250279231545228 2313 94-95 -1.6061446678738172 2313 96-97 -1.5782659770636194 2313 98-99 -1.5474080658428697 2313 100-101 -1.5255245629717251 2315 1 -3.884793551824192 2315 2 -5.679917671423013 2315 3 -4.918924850121055 2315 4 -5.141526806675422 2315 5 -4.028622459324843 2315 6 -3.394393172289341 2315 7 -3.064551820194474 2315 8 -2.9795159640356808 2315 9 -3.3091486910483567 2315 10-11 -3.207787711343144 2315 12-13 -3.2079770489862867 2315 14-15 -3.5000483992533873 2315 16-17 -3.5002828338910987 2315 18-19 -3.4769719759111695 2315 20-21 -3.5437619394213407 2315 22-23 -3.5963252661822693 2315 24-25 -3.639003295105269 2315 26-27 -3.5759521640541614 2315 28-29 -3.630597069048921 2315 30-31 -3.6865239328694877 2315 32-33 -3.7907558697458157 2315 34-35 -3.8038896581302453 2315 36-37 -3.6416906127718818 2315 38-39 -3.606193727660397 2315 40-41 -3.6093416570105745 2315 42-43 -3.629692863869515 2315 44-45 -3.621042779957083 2315 46-47 -3.706590196982873 2315 48-49 -3.7229024730202553 2315 50-51 -3.6305987048184036 2315 52-53 -3.600212001762287 2315 54-55 -3.56476808111357 2315 56-57 -3.621165298818447 2315 58-59 -3.612585022623641 2315 60-61 -3.665572667150439 2315 62-63 -3.707820589155588 2315 64-65 -3.5677431260938697 2315 66-67 -3.6155601447633323 2315 68-69 -3.6540643922814766 2315 70-71 -3.67214527067172 2315 72-73 -3.6940810694116806 2315 74-75 -3.719188413827993 2315 76-77 -3.62029088685901 2315 78-79 -3.7528046346523247 2315 80-81 -3.806535939936044 2315 82-83 -3.7268426775873564 2315 84-85 -3.757232635803632 2315 86-87 -3.9126194370940652 2315 88-89 -3.886505135461764 2315 90-91 -3.9381688759150393 2315 92-93 -3.846547018123232 2315 94-95 -3.8397292472867406 2315 96-97 -3.8147895864857553 2315 98-99 -3.6466768861531165 2315 100-101 -3.6617756864498148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1083722.0 3 229724.0 4 140057.0 5 126924.0 6 187486.0 7 180911.0 8 168328.0 9 239150.0 10 335715.0 11 321498.0 12 247260.0 13 188913.0 14 159871.0 15 146556.0 16 141073.0 17 136181.0 18 132194.0 19 129442.0 20 130139.0 21 135258.0 22 148229.0 23 170957.0 24 197631.0 25 230801.0 26 271655.0 27 318532.0 28 374045.0 29 441164.0 30 524201.0 31 636039.0 32 763660.0 33 955380.0 34 1240209.0 35 1683858.0 36 2464239.0 37 4138931.0 38 5762978.0 39 1977275.0 40 5723.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.456302492310694 17.84038147902259 11.674566601577565 47.02874942708915 2 14.906390998336574 19.142713362864548 52.726340234608124 13.224555404190749 3 19.89819730394794 23.30744819205907 35.006360481587514 21.78799402240548 4 22.196847292466707 30.20136646434841 29.90530576492571 17.696480478259172 5 20.96272902276596 33.563406931419024 30.969305426210436 14.504558619604577 6 16.236703563088696 36.575002469453395 31.799614299511923 15.388679667945986 7 13.402191432057888 20.025544734418716 50.41283446276519 16.1594293707582 8 13.76696809224641 21.916183927862974 43.91891091174412 20.397937068146497 9 15.23649590569539 22.43745711515145 43.322463612423796 19.00358336672937 10-11 18.51303904375283 33.868755763700776 31.687356277284547 15.930848915261848 12-13 17.506742814048405 26.797286987666997 38.02825792344252 17.667712274842085 14-15 18.5188707144797 28.066329298693894 36.479682855641194 16.93511713118522 16-17 19.45824359379101 27.706802040312184 34.891285787249075 17.94366857864774 18-19 19.423985456931593 28.356390619871487 34.19604142846415 18.023582494732775 20-21 19.642349413543982 28.032209322381803 33.78101000469544 18.54443125937878 22-23 18.897987388198974 29.85545646177722 33.87092082337726 17.37563532664655 24-25 18.76018637592023 29.62086292051067 33.21533128319821 18.403619420370887 26-27 18.962228472939696 29.81944514082101 32.778213828590964 18.44011255764833 28-29 19.002993783381072 29.851182451979568 32.477050792317684 18.66877297232167 30-31 18.674047350226626 30.111843701136376 32.32460958877398 18.88949935986302 32-33 18.76614336023505 30.02264367623234 32.249906319386575 18.961306644146035 34-35 19.260896003697987 30.93100433470557 30.816368771977466 18.99173088961898 36-37 19.688529037778405 30.576507118761302 31.137047148843305 18.59791669461699 38-39 19.37522984626301 30.564356618032296 31.23976622448926 18.82064731121544 40-41 19.431420507245445 30.47863481221541 31.11997186989929 18.969972810639852 42-43 19.32691462913847 30.314236675910294 31.586201102343814 18.772647592607424 44-45 19.364410038433373 30.052707794993466 31.590052049870664 18.992830116702496 46-47 19.247818288115308 29.889989866241244 31.792350213035686 19.06984163260776 48-49 19.261846631543573 29.744348236638718 31.63098235589757 19.36282277592014 50-51 18.894028628863843 30.165247538263586 32.18092528671138 18.759798546161196 52-53 19.989799965619497 29.83080439844457 31.376923901817648 18.802471734118285 54-55 19.47675344959895 30.272306704194317 30.977293681113956 19.273646165092774 56-57 20.062137974244752 30.095127689343173 30.689732856761566 19.153001479650513 58-59 19.28643242787127 30.959257703544818 30.69915930814599 19.055150560437927 60-61 19.32570742089636 30.859839838196695 30.08950545329182 19.72494728761512 62-63 19.606431224097186 30.891272223215154 30.616709438083383 18.885587114604277 64-65 19.87735002492702 30.612494012975798 30.37791699644573 19.132238965651453 66-67 19.68945030727219 31.170586266987772 29.932519710993315 19.207443714746727 68-69 19.79231411598705 31.031237217724545 30.312595900622075 18.863852765666326 70-71 19.697762840316198 31.199023393381154 29.9665798204596 19.136633945843048 72-73 19.424286685023496 31.22847032261666 30.12501133318476 19.222231659175083 74-75 19.345213612268743 30.514845657512936 31.245953063231074 18.893987666987247 76-77 19.18374758599387 31.034123587879954 30.570277718494452 19.211851107631723 78-79 19.571966292675494 31.146569238111706 30.25594596898283 19.025518500229975 80-81 19.450209270540295 31.034670900812138 30.393056087795973 19.12206374085159 82-83 19.805354502269815 31.486177100193668 29.778597295435333 18.929871102101185 84-85 19.49629792115946 30.946245123137956 30.199559898283653 19.357897057418935 86-87 19.440694209986926 31.738372119538695 30.020996700598257 18.799936969876125 88-89 19.354624747431696 31.26713647751548 30.5193877907571 18.85885098429572 90-91 19.305214866946713 31.766699294600087 30.193951008218033 18.73413483023517 92-93 19.870092522223146 30.970959372456345 30.117518318960908 19.041429786359604 94-95 19.400997594527773 31.42164732130881 30.034724817600367 19.14263026656305 96-97 19.562553988442748 31.49418227987609 29.97288104670559 18.970382684975576 98-99 19.642715786917687 31.372992546571528 29.69697575452025 19.28731591199054 100-101 20.022067079454146 32.14257660096615 28.90279242091218 18.932563898667524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6474.0 1 8684.5 2 9549.5 3 9435.5 4 12634.0 5 16970.0 6 20886.5 7 24830.0 8 29747.5 9 34969.5 10 40427.0 11 46153.5 12 52167.5 13 57862.5 14 63448.5 15 69556.5 16 75870.0 17 82769.0 18 92593.5 19 105772.5 20 123735.5 21 152490.0 22 196438.0 23 232162.5 24 268537.5 25 336886.5 26 429667.5 27 531925.5 28 607108.5 29 658607.0 30 735076.5 31 820499.0 32 904243.0 33 987996.5 34 1061496.5 35 1164890.0 36 1265048.5 37 1379337.5 38 1427330.5 39 1198074.5 40 1023926.0 41 998121.0 42 889693.0 43 796585.5 44 744421.0 45 713605.5 46 671293.5 47 637774.0 48 620725.5 49 592345.0 50 547561.5 51 497851.5 52 444431.5 53 391227.5 54 347308.5 55 298113.5 56 253809.0 57 211422.5 58 174532.0 59 142756.5 60 110819.5 61 83505.0 62 61745.5 63 44632.0 64 31238.0 65 22539.0 66 16945.0 67 13514.5 68 11179.5 69 9570.0 70 10196.0 71 13522.5 72 13866.5 73 17549.5 74 18482.0 75 11679.5 76 8335.5 77 7673.5 78 6729.5 79 4368.0 80 2954.5 81 2103.5 82 1565.5 83 1191.0 84 961.0 85 757.5 86 530.5 87 369.5 88 273.5 89 188.5 90 117.0 91 77.5 92 52.0 93 28.5 94 13.0 95 7.5 96 4.0 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052110650713512056 2 0.005404618916858536 3 0.005922003234656977 4 0.0030149733627103403 5 0.0232376280288897 6 0.1041691907763106 7 0.11110735169988106 8 0.11861500759196349 9 0.12610777472669918 10-11 0.1333157943771789 12-13 0.143155401888691 14-15 0.14707672835488275 16-17 0.15368361442748876 18-19 0.15375805821422234 20-21 0.16025327860672794 22-23 0.1300067680568709 24-25 0.14296557023252032 26-27 0.15091244446633093 28-29 0.15754166367495698 30-31 0.16782235062286557 32-33 0.17144031865811798 34-35 0.1668489981113239 36-37 0.12130242829304604 38-39 0.18265155294019644 40-41 0.19328398678042125 42-43 0.1845945357739431 44-45 0.20217071382174337 46-47 0.19564199372520769 48-49 0.1996414861674697 50-51 0.19586160289607174 52-53 0.19092411874096646 54-55 0.18989307229470628 56-57 0.19786600185387362 58-59 0.19791439031525046 60-61 0.2050349385163182 62-63 0.211876322517135 64-65 0.3788369863085593 66-67 0.18529802955857552 68-69 0.3415983430897499 70-71 0.011527620375696202 72-73 0.012746637383458716 74-75 0.025532357754952568 76-77 0.030019457000319622 78-79 0.01986718558452647 80-81 0.023003130100678896 82-83 0.041271635365101554 84-85 0.020574401558495565 86-87 0.021352339129861564 88-89 0.02637357254504212 90-91 0.009022586952110945 92-93 0.013824211196427412 94-95 0.03202757814745818 96-97 0.030927671198469404 98-99 0.008804838875915199 100-101 0.017230014439489093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.6865909E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.00065207469741 #Duplication Level Percentage of deduplicated Percentage of total 1 76.82078949699662 26.119569357909512 2 9.492569913227168 6.45507133828755 3 4.054311641999304 4.135477186260404 4 2.2438579156270655 3.051705291771664 5 1.4634468945484047 2.487907434566835 6 1.0091645344993414 2.058735133418165 7 0.72460058841763 1.72458247499362 8 0.5311118895937093 1.4446520456648648 9 0.4559946015920299 1.3953702417003786 >10 2.7854690036319916 17.927240096429447 >50 0.23707704007142846 5.591753195024764 >100 0.15354470645912507 10.104733060510396 >500 0.01532436708323846 3.5812634530381504 >1k 0.011054068675796653 7.317522969958389 >5k 0.001084566492610892 2.49843133719911 >10k+ 5.987710844611549E-4 4.105985383266666 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 79547 0.29608899516483883 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 67201 0.2501348456141945 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 62299 0.23188867348579198 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGATTACGTCTACAAAA 51691 0.19240368900229654 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 38438 0.14307351372328403 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 31479 0.11717079812933187 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 29452 0.10962592034388266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.051596988585050296 0.0 0.0 7.444378673358865E-6 0.0 2 0.05188731935331129 1.8610946683397164E-5 0.0 7.444378673358865E-6 0.0 3 0.05249031402585336 2.2333136020076595E-5 0.0 7.444378673358865E-6 0.0 4 0.05423229863541933 2.605532535675603E-5 0.0 1.488875734671773E-5 0.0 5 0.05490601490535831 2.605532535675603E-5 0.0 1.488875734671773E-5 0.0 6 0.05566161934070424 2.605532535675603E-5 0.0 1.8610946683397164E-5 0.0 7 0.05616783709049264 2.605532535675603E-5 0.0 1.8610946683397164E-5 0.0 8 0.058144319628269416 2.605532535675603E-5 0.0 2.2333136020076595E-5 0.0 9 0.058702648028771336 2.605532535675603E-5 0.0 2.2333136020076595E-5 0.0 10-11 0.06109601577225621 2.605532535675603E-5 0.0 2.2333136020076595E-5 0.0 12-13 0.06480889963559394 2.977751469343546E-5 0.0 2.2333136020076595E-5 0.0 14-15 0.06845664518553979 2.977751469343546E-5 0.0 2.2333136020076595E-5 0.0 16-17 0.07258641425458562 3.1638609361775175E-5 0.0 2.605532535675603E-5 0.0 18-19 0.07661382311687276 3.536079869845461E-5 0.0 3.536079869845461E-5 0.0 20-21 0.08039928967227575 3.722189336679433E-5 0.0 3.9082988035134045E-5 0.0 22-23 0.08371576037125712 4.466627204015319E-5 0.0 4.8388461376832625E-5 0.0 24-25 0.08680889971003772 5.211065071351206E-5 0.0 5.9555029386870915E-5 0.0 26-27 0.08929346109227125 5.211065071351206E-5 0.0 6.513831339189006E-5 0.0 28-29 0.09215582469217773 5.211065071351206E-5 0.0 7.444378673358865E-5 0.0 30-31 0.0947222742398182 5.583284005019149E-5 0.0 7.816597607026809E-5 0.0 32-33 0.09680856136302703 5.583284005019149E-5 0.0 7.816597607026809E-5 0.0 34-35 0.09949970425344626 5.583284005019149E-5 0.0 8.00270707386078E-5 0.0 36-37 0.1016436853113736 5.7693934718531205E-5 0.0 9.305473341698581E-5 1.8610946683397163E-6 38-39 0.10384908249335617 5.955502938687092E-5 1.8610946683397163E-6 9.677692275366525E-5 7.444378673358865E-6 40-41 0.10625547789951942 6.327721872355035E-5 3.7221893366794326E-6 1.0049911209034468E-4 7.444378673358865E-6 42-43 0.10836223706407998 6.513831339189006E-5 3.7221893366794326E-6 1.0980458543204326E-4 7.444378673358865E-6 44-45 0.11058438409807761 7.072159739690922E-5 3.7221893366794326E-6 1.1166568010038297E-4 9.305473341698582E-6 46-47 0.11302614030293931 7.072159739690922E-5 3.7221893366794326E-6 1.3027662678378012E-4 1.488875734671773E-5 48-49 0.1154902296438211 7.072159739690922E-5 3.7221893366794326E-6 1.3958210012547873E-4 1.8610946683397164E-5 50-51 0.11843262031446619 7.258269206524893E-5 3.7221893366794326E-6 1.4144319479381844E-4 1.8610946683397164E-5 52-53 0.12185889559887961 7.816597607026809E-5 3.7221893366794326E-6 1.5260976280385676E-4 1.8610946683397164E-5 54-55 0.12552525209550885 8.188816540694752E-5 3.7221893366794326E-6 1.600541414772156E-4 1.8610946683397164E-5 56-57 0.12954521657912266 8.188816540694752E-5 3.7221893366794326E-6 1.6749852015057447E-4 1.8610946683397164E-5 58-59 0.13430589674073562 8.188816540694752E-5 3.7221893366794326E-6 1.7866508816061276E-4 1.8610946683397164E-5 60-61 0.13975704302430267 8.561035474362696E-5 3.7221893366794326E-6 1.8797056150231137E-4 1.8610946683397164E-5 62-63 0.1457237125309998 8.933254408030638E-5 3.7221893366794326E-6 1.954149401756702E-4 1.8610946683397164E-5 64-65 0.1526116238985251 8.933254408030638E-5 3.7221893366794326E-6 2.0285931884902908E-4 1.8610946683397164E-5 66-67 0.1605417482803206 9.305473341698581E-5 3.7221893366794326E-6 2.1216479219072766E-4 1.8610946683397164E-5 68-69 0.1699086377460744 9.305473341698581E-5 3.7221893366794326E-6 2.1402588685906737E-4 2.0472041351736878E-5 70-71 0.18107706685078104 9.305473341698581E-5 3.7221893366794326E-6 2.1960917086408653E-4 2.4194230688416312E-5 72-73 0.19349615157261196 9.305473341698581E-5 3.7221893366794326E-6 2.1960917086408653E-4 2.605532535675603E-5 74-75 0.20863987888889224 9.305473341698581E-5 3.7221893366794326E-6 2.3077573887412482E-4 2.605532535675603E-5 76-77 0.2269511893306867 9.491582808532554E-5 3.7221893366794326E-6 2.7544201091427804E-4 2.605532535675603E-5 78-79 0.24865713644753282 1.0422130142702412E-4 3.7221893366794326E-6 2.8102529491929717E-4 2.605532535675603E-5 80-81 0.2740461899130232 1.0422130142702412E-4 3.7221893366794326E-6 3.0335843093937375E-4 2.605532535675603E-5 82-83 0.3048361401060355 1.0794349076370355E-4 3.7221893366794326E-6 3.201082829544312E-4 2.605532535675603E-5 84-85 0.34252516823458307 1.0794349076370355E-4 3.7221893366794326E-6 3.275526616277901E-4 2.605532535675603E-5 86-87 0.3893633377526887 1.0794349076370355E-4 3.7221893366794326E-6 3.312748509644695E-4 2.605532535675603E-5 88-89 0.44377244038160035 1.0980458543204326E-4 3.7221893366794326E-6 3.312748509644695E-4 2.605532535675603E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 20780 0.0 79.36064 1 GCGTGTT 28000 0.0 59.060135 2 CGTGTTT 40745 0.0 41.51928 3 CCCGAAA 24570 0.0 34.206924 1 TCGTCTA 24440 0.0 33.030605 44-45 ACTCGTC 24595 0.0 32.938503 42-43 CCTTTAG 23170 0.0 32.47152 5 CTAACGA 24340 0.0 32.229885 94-95 TAGGCAT 23335 0.0 31.901337 9 TTAGGCA 23655 0.0 31.449066 8 GTTTTTT 312685 0.0 31.320799 1 TTTAGGC 25555 0.0 30.076874 7 GCCTAAC 27985 0.0 28.337984 92-93 TCGTACC 2645 0.0 27.994534 2 CTTTAGG 27785 0.0 27.385769 6 CCGAAAC 31485 0.0 26.909698 2 CCCGTAT 2880 0.0 26.87454 1 GTCTATG 30955 0.0 26.331459 46-47 CCAAACG 34980 0.0 26.153128 8 CGCGCGT 1130 0.0 25.212662 1 >>END_MODULE