FastQCFastQC Report
Fri 27 May 2016
SRR1233035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73746655
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT510300.074.357261
GCGTGTT709100.052.9745372
GTTTTTT9811950.042.5778121
CGTGTTT1061450.036.024443
CGCGCGT29100.023.6125071
CGCGGGT26050.020.515541
TTTAGGC446950.020.5068867
TTAGGCA439700.020.0791198
TAGGCAT443100.019.5612629
CGGACGT20200.019.3702431
GCGCGTT31000.019.170832
CCGGTGT231150.018.9906416-17
ACTCGTC111750.018.90846642-43
CATTCCG247300.018.58149112-13
TCGTCTA117400.018.3164844-45
CTGGAAT1206500.017.8725951
TCCGGTG226900.017.8404716-17
TTCCGGT257050.017.38175414-15
CTCGTAT69050.017.3453331
TATGGCG816900.017.2944747
Produced by FastQC (version 0.11.5)