##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233035_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73746655 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.753558924130186 31.0 30.0 34.0 25.0 34.0 2 29.934826874520613 31.0 30.0 34.0 23.0 34.0 3 29.9221530657899 31.0 30.0 34.0 23.0 34.0 4 33.24561949284344 37.0 35.0 37.0 25.0 37.0 5 33.12252692681451 37.0 33.0 37.0 25.0 37.0 6 33.23449173389627 37.0 35.0 37.0 26.0 37.0 7 33.2895385831398 37.0 35.0 37.0 26.0 37.0 8 33.19455773282191 37.0 35.0 37.0 25.0 37.0 9 34.67844287988384 39.0 35.0 39.0 26.0 39.0 10-11 34.56387191391935 39.0 35.0 39.0 26.0 39.0 12-13 34.47215172674611 38.5 35.0 39.0 25.0 39.0 14-15 35.35947681288053 39.0 34.5 41.0 25.0 41.0 16-17 35.10574644368616 39.0 34.0 41.0 24.5 41.0 18-19 34.76599956296323 39.0 34.0 41.0 19.0 41.0 20-21 34.535539035634905 39.0 34.0 41.0 18.0 41.0 22-23 34.270377863240036 39.0 34.0 41.0 18.0 41.0 24-25 33.90983757161596 39.0 33.0 41.0 16.0 41.0 26-27 33.63751720671263 39.0 33.0 41.0 10.5 41.0 28-29 33.494377595838074 39.0 33.0 41.0 10.0 41.0 30-31 33.28922067719546 39.0 33.0 41.0 8.5 41.0 32-33 33.138668194509975 39.0 33.0 41.0 8.0 41.0 34-35 32.95788966292776 38.5 32.0 41.0 4.5 41.0 36-37 32.82526526796368 38.0 32.0 41.0 2.0 41.0 38-39 32.68972457123648 38.0 31.5 41.0 2.0 41.0 40-41 32.555987793615856 38.0 31.0 41.0 2.0 41.0 42-43 32.424597732602244 38.0 31.0 40.5 2.0 41.0 44-45 32.15207695860917 38.0 31.0 40.0 2.0 41.0 46-47 32.05275641993525 38.0 30.5 40.0 2.0 41.0 48-49 31.976335455486083 38.0 30.0 40.0 2.0 41.0 50-51 31.430167720827473 37.5 30.5 39.5 2.0 40.5 52-53 31.478801424959546 38.0 30.0 40.0 2.0 40.5 54-55 31.759036820042887 38.0 30.5 40.0 2.0 41.0 56-57 31.66552734493517 38.0 30.0 40.0 2.0 41.0 58-59 31.525193861335133 38.0 30.0 40.0 2.0 41.0 60-61 31.423509120515362 38.0 30.0 40.0 2.0 41.0 62-63 31.213773553525918 38.0 29.5 40.0 2.0 41.0 64-65 30.97712473196242 37.0 29.0 40.0 2.0 41.0 66-67 30.733560742517746 37.0 29.0 40.0 2.0 41.0 68-69 30.42984810633106 36.0 28.5 39.5 2.0 41.0 70-71 30.102745229597193 36.0 28.0 39.0 2.0 41.0 72-73 29.76893217055065 35.5 28.0 39.0 2.0 41.0 74-75 29.39169798955627 35.0 28.0 39.0 2.0 40.0 76-77 28.983398236842064 35.0 27.0 37.5 2.0 40.0 78-79 28.611134566035574 35.0 26.0 37.0 2.0 39.0 80-81 28.217374849069426 34.5 26.0 37.0 2.0 39.0 82-83 27.83026874235855 34.0 26.0 36.0 2.0 38.5 84-85 27.485591258342495 34.0 26.0 36.0 2.0 37.0 86-87 27.11514865318298 34.0 25.0 35.0 2.0 37.0 88-89 26.7939799439039 34.0 25.0 35.0 2.0 36.5 90-91 26.483377435898618 34.0 24.0 35.0 2.0 36.0 92-93 26.162403413415838 34.0 22.0 35.0 2.0 36.0 94-95 25.810255421076384 34.0 20.0 35.0 2.0 36.0 96-97 25.45376709628389 34.0 17.5 35.0 2.0 35.0 98-99 25.01960831308213 34.0 2.0 35.0 2.0 35.0 100-101 23.354418264801296 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2191170457359561 1101 2 1.5158223776625483 1101 3 1.58393573777197 1101 4 1.6648104738034064 1101 5 1.7157095851213384 1101 6 1.4153412997346067 1101 7 1.6227330928815036 1101 8 1.730080553692062 1101 9 1.6444719885700465 1101 10-11 1.7977794255870094 1101 12-13 1.7926865818900382 1101 14-15 1.9125220848451505 1101 16-17 1.9371724904377388 1101 18-19 2.095707795066417 1101 20-21 2.1178904255045765 1101 22-23 2.04187514752568 1101 24-25 2.100299077197178 1101 26-27 1.983083181951983 1101 28-29 2.0415373849551344 1101 30-31 2.0493597464837663 1101 32-33 1.9517020684864619 1101 34-35 2.012261389670549 1101 36-37 1.8874941918702746 1101 38-39 1.809652346101899 1101 40-41 1.9473184903947924 1101 42-43 1.9672740248318448 1101 44-45 1.8789730220607908 1101 46-47 1.9558051816771638 1101 48-49 2.0208506531923334 1101 50-51 1.9719194411510053 1101 52-53 1.9489915947887084 1101 54-55 2.0772787691819232 1101 56-57 2.1368123782086954 1101 58-59 2.212669901543766 1101 60-61 2.196100243979174 1101 62-63 2.1855589550586068 1101 64-65 2.185995283707676 1101 66-67 2.147612043949131 1101 68-69 2.1920897696239052 1101 70-71 2.1910946065254358 1101 72-73 2.194563257378494 1101 74-75 2.1312501540208615 1101 76-77 2.103453097273274 1101 78-79 2.132847550054045 1101 80-81 2.060716723743255 1101 82-83 1.9382338908709116 1101 84-85 1.9786332994498608 1101 86-87 2.0377034441459294 1101 88-89 2.083672110986427 1101 90-91 1.9956408437103264 1101 92-93 2.0707729060723565 1101 94-95 2.120948626306852 1101 96-97 2.1439780397730033 1101 98-99 2.1725467071648836 1101 100-101 2.179889913966015 1102 1 1.2511411368402783 1102 2 1.5487374873102198 1102 3 1.5427297344610267 1102 4 1.6091315449013592 1102 5 1.6943143152703968 1102 6 1.630563964451241 1102 7 1.7247569185514422 1102 8 1.7877546210615733 1102 9 1.9178152617255222 1102 10-11 1.8864766869359784 1102 12-13 1.8349872235042568 1102 14-15 2.1060608939336802 1102 16-17 2.0894418027063395 1102 18-19 2.0693517277251345 1102 20-21 2.1006411015493995 1102 22-23 1.972179910415889 1102 24-25 1.8961260312623338 1102 26-27 1.9244947061683249 1102 28-29 1.9488674376359683 1102 30-31 1.993401957279488 1102 32-33 1.8887127880031045 1102 34-35 1.858573483122349 1102 36-37 1.8705088571023083 1102 38-39 1.9029361490779166 1102 40-41 1.9395718434012963 1102 42-43 1.9687896618829939 1102 44-45 1.8643760115943309 1102 46-47 1.971057608187941 1102 48-49 1.9863480775177536 1102 50-51 1.9206811123823577 1102 52-53 1.9551580347975133 1102 54-55 2.006465086915533 1102 56-57 2.062617087784119 1102 58-59 2.225091091806359 1102 60-61 2.1408279714589753 1102 62-63 2.1437609232234642 1102 64-65 2.1141140456849357 1102 66-67 2.143720701846462 1102 68-69 2.060547405821687 1102 70-71 2.1371242570519904 1102 72-73 2.068292079670723 1102 74-75 2.034719033175275 1102 76-77 2.008395533706075 1102 78-79 2.050150253868999 1102 80-81 2.0864965160526268 1102 82-83 1.9914458449564343 1102 84-85 2.0753621767989188 1102 86-87 2.044614887490372 1102 88-89 2.040909608926661 1102 90-91 2.1004384694232137 1102 92-93 2.1166297816720174 1102 94-95 2.1346359169720586 1102 96-97 2.22859999840167 1102 98-99 2.2384495170815484 1102 100-101 2.215249162012565 1103 1 1.1804975581791624 1103 2 1.4796297261460545 1103 3 1.4353590396876221 1103 4 1.5338459440607508 1103 5 1.6033912776387425 1103 6 1.5071576974005794 1103 7 1.578931470794096 1103 8 1.6537548606539332 1103 9 1.6583124623018435 1103 10-11 1.7055342237269713 1103 12-13 1.6549109905295865 1103 14-15 1.946440093197971 1103 16-17 1.9482965007076558 1103 18-19 1.9838737729511848 1103 20-21 1.9625600166641988 1103 22-23 1.8546913071776885 1103 24-25 1.8500567624755107 1103 26-27 1.850717155814678 1103 28-29 1.90353942058384 1103 30-31 1.8939776321944635 1103 32-33 1.8021655297135197 1103 34-35 1.893553343365113 1103 36-37 1.8537363788057348 1103 38-39 1.8981458944059852 1103 40-41 1.93740992145284 1103 42-43 1.9487666464370434 1103 44-45 1.8978921062682588 1103 46-47 1.9027715985158693 1103 48-49 1.9746808778491882 1103 50-51 1.9445276916998218 1103 52-53 1.9416530518668722 1103 54-55 2.0689416084442414 1103 56-57 2.0620911950119947 1103 58-59 2.209949938343282 1103 60-61 2.1416547414292566 1103 62-63 2.070358385148875 1103 64-65 2.059062043415146 1103 66-67 2.0746485892322752 1103 68-69 2.085126813452767 1103 70-71 2.1532662276206445 1103 72-73 2.151177007883806 1103 74-75 2.1107580594808795 1103 76-77 2.0773628679064515 1103 78-79 2.114975076480629 1103 80-81 2.182158515980454 1103 82-83 2.123250358737007 1103 84-85 2.0904826397060248 1103 86-87 2.036212267561851 1103 88-89 2.0450889542080617 1103 90-91 2.138195912242278 1103 92-93 2.114557973618261 1103 94-95 2.2015185113890574 1103 96-97 2.217609899202621 1103 98-99 2.198958195331862 1103 100-101 2.2457955448283258 1104 1 1.1615838732098318 1104 2 1.357686336504333 1104 3 1.337388500981902 1104 4 1.3954148046959887 1104 5 1.535810537094818 1104 6 1.4077612335484133 1104 7 1.4939686173774263 1104 8 1.5940377360630862 1104 9 1.6245354466594577 1104 10-11 1.5362578097464308 1104 12-13 1.5321252362898576 1104 14-15 1.8054620184370052 1104 16-17 1.7815139087035732 1104 18-19 1.7655976458358609 1104 20-21 1.759088458070039 1104 22-23 1.665975800093321 1104 24-25 1.674054648393998 1104 26-27 1.6678592629167497 1104 28-29 1.6504480256122136 1104 30-31 1.653839840068919 1104 32-33 1.6920726864613442 1104 34-35 1.7064620369308514 1104 36-37 1.648906876484233 1104 38-39 1.6467287895198979 1104 40-41 1.651870880550348 1104 42-43 1.6729926097085013 1104 44-45 1.6463879568123048 1104 46-47 1.634387632599065 1104 48-49 1.678650186959878 1104 50-51 1.5594490654031894 1104 52-53 1.658844292330869 1104 54-55 1.8393698840923705 1104 56-57 1.7532855158309744 1104 58-59 1.8931169933636092 1104 60-61 1.8707181245232434 1104 62-63 1.828230709498964 1104 64-65 1.7228694584390212 1104 66-67 1.8030900352991424 1104 68-69 1.7926270452408986 1104 70-71 1.7668462611312101 1104 72-73 1.8077871080889167 1104 74-75 1.7804751721047296 1104 76-77 1.7854827580338934 1104 78-79 1.8370855805175133 1104 80-81 1.8354030194672006 1104 82-83 1.8435088852772985 1104 84-85 1.8046025731662851 1104 86-87 1.7427951003390376 1104 88-89 1.7567439077939824 1104 90-91 1.839739860669198 1104 92-93 1.810254204643197 1104 94-95 1.8212356727350034 1104 96-97 1.7951771786447281 1104 98-99 1.840219747084383 1104 100-101 1.87056210999663 1105 1 0.9599335717096444 1105 2 1.1865982849142043 1105 3 1.1749874112070735 1105 4 1.21492895767458 1105 5 1.2934956322553148 1105 6 1.182700099181801 1105 7 1.251731597329659 1105 8 1.3051557651834287 1105 9 1.405437872679009 1105 10-11 1.3298434102813133 1105 12-13 1.3350214210299072 1105 14-15 1.5930101106493808 1105 16-17 1.4809342194433555 1105 18-19 1.558507808793408 1105 20-21 1.5664122873852833 1105 22-23 1.497966424540877 1105 24-25 1.5656090659848374 1105 26-27 1.4993643311235871 1105 28-29 1.4625828756266088 1105 30-31 1.4825974355187341 1105 32-33 1.4369697706337234 1105 34-35 1.4325534671867715 1105 36-37 1.3406352341762329 1105 38-39 1.364644016609013 1105 40-41 1.4110679865188587 1105 42-43 1.4438306829205203 1105 44-45 1.3641759682301 1105 46-47 1.4460089933834865 1105 48-49 1.364815324474968 1105 50-51 1.3358171216315817 1105 52-53 1.4189829745918843 1105 54-55 1.518160226849865 1105 56-57 1.5562774310845882 1105 58-59 1.63839276715375 1105 60-61 1.645313510177374 1105 62-63 1.6217186954437004 1105 64-65 1.5523542404406712 1105 66-67 1.5828954759160894 1105 68-69 1.6256190295321247 1105 70-71 1.6730410135441538 1105 72-73 1.6713439510996544 1105 74-75 1.6565578783940538 1105 76-77 1.6956384976595125 1105 78-79 1.627580928571323 1105 80-81 1.6060813652846413 1105 82-83 1.556446979385644 1105 84-85 1.5957996775808958 1105 86-87 1.5987860793839275 1105 88-89 1.5404555822346424 1105 90-91 1.5936333947179548 1105 92-93 1.6026747255253966 1105 94-95 1.5771806298241025 1105 96-97 1.589202801045733 1105 98-99 1.544355285230676 1105 100-101 1.5512852781137312 1106 1 0.9439963608179447 1106 2 1.1607534687574628 1106 3 1.1333733502102348 1106 4 1.2382187167045302 1106 5 1.3031805873206395 1106 6 1.201608511980531 1106 7 1.2959517808986334 1106 8 1.3203003983052 1106 9 1.3674698630744118 1106 10-11 1.268280231989685 1106 12-13 1.2456838049932628 1106 14-15 1.3857344846714668 1106 16-17 1.3864418861083863 1106 18-19 1.431284742651556 1106 20-21 1.351225938095439 1106 22-23 1.331578414874798 1106 24-25 1.2444153391497537 1106 26-27 1.27750063293945 1106 28-29 1.1819365451426762 1106 30-31 1.2708128896102622 1106 32-33 1.328250923858377 1106 34-35 1.3332380203714322 1106 36-37 1.300241077272858 1106 38-39 1.2731778291739175 1106 40-41 1.287338479144438 1106 42-43 1.3299418109892898 1106 44-45 1.26746203287518 1106 46-47 1.3604739636353855 1106 48-49 1.3089572208348343 1106 50-51 1.2023049607591396 1106 52-53 1.20805119884011 1106 54-55 1.2508052319639447 1106 56-57 1.2959097379230329 1106 58-59 1.449822293828408 1106 60-61 1.3726614820891854 1106 62-63 1.4535362418279725 1106 64-65 1.428272913361539 1106 66-67 1.453060403874467 1106 68-69 1.2887508256973241 1106 70-71 1.3973795172600632 1106 72-73 1.3761647453693122 1106 74-75 1.4485982451234989 1106 76-77 1.4138508794502371 1106 78-79 1.4709994096767858 1106 80-81 1.4679056374437067 1106 82-83 1.415722251199039 1106 84-85 1.3592859356473532 1106 86-87 1.4269740469216856 1106 88-89 1.403107250519227 1106 90-91 1.4169484271961856 1106 92-93 1.4682514087768723 1106 94-95 1.3896605874747188 1106 96-97 1.4470442846771547 1106 98-99 1.4732352954197836 1106 100-101 1.3783355252974339 1107 1 0.7697872474602825 1107 2 1.0041073048626643 1107 3 0.9193039596227699 1107 4 0.9933099774957483 1107 5 1.018071364627275 1107 6 0.9668503267168873 1107 7 1.0102552140641308 1107 8 1.0996136441534148 1107 9 1.162996842659446 1107 10-11 1.1303509374442768 1107 12-13 1.0972475214905373 1107 14-15 1.2084186719577943 1107 16-17 1.1998193459300666 1107 18-19 1.171915110903612 1107 20-21 1.2098764650118596 1107 22-23 1.185590550722253 1107 24-25 1.102949761249583 1107 26-27 1.064032968010892 1107 28-29 1.1041765849029161 1107 30-31 1.1602200115111998 1107 32-33 1.175706176575332 1107 34-35 1.1659409313980902 1107 36-37 1.0739475994366217 1107 38-39 1.0018480095827726 1107 40-41 1.0253249593844345 1107 42-43 1.0479714353392993 1107 44-45 1.0111128407692576 1107 46-47 1.054037940602285 1107 48-49 1.038369528035851 1107 50-51 0.9903062681287231 1107 52-53 0.9919039102209979 1107 54-55 1.179636172749717 1107 56-57 1.1616009774668754 1107 58-59 1.2446951692517167 1107 60-61 1.249915788613805 1107 62-63 1.1951246241091091 1107 64-65 1.1933290325719739 1107 66-67 1.1837993728425324 1107 68-69 1.1488920717776736 1107 70-71 1.1488821447832755 1107 72-73 1.1257785111905676 1107 74-75 1.173856446418828 1107 76-77 1.1276868016603245 1107 78-79 1.1331425308115186 1107 80-81 1.1627843576995822 1107 82-83 1.1494995833819779 1107 84-85 1.1006622926928458 1107 86-87 1.137089310992888 1107 88-89 1.1770002372362356 1107 90-91 1.1734189833147894 1107 92-93 1.1275014307874685 1107 94-95 1.1382433574811337 1107 96-97 1.1902904987483822 1107 98-99 1.2250452335237298 1107 100-101 1.2131182254433988 1108 1 0.6470486796192638 1108 2 0.9086193319128313 1108 3 0.8312956576525004 1108 4 0.9167189834985052 1108 5 0.9961216671997377 1108 6 0.9173431264835514 1108 7 0.959452711206545 1108 8 0.9832277312106683 1108 9 0.9561365549365064 1108 10-11 0.9538210779855234 1108 12-13 0.8796850140966797 1108 14-15 1.0577984464197883 1108 16-17 0.9217625439633537 1108 18-19 1.0062542747622985 1108 20-21 0.9374334204780439 1108 22-23 0.7803573255470013 1108 24-25 0.8862703393013902 1108 26-27 0.8564910478484933 1108 28-29 0.9202139196913421 1108 30-31 0.8881400043637697 1108 32-33 0.8592261334363229 1108 34-35 0.857608462211175 1108 36-37 0.8660117038630091 1108 38-39 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-5.035107491890802 2316 14-15 -6.46683455745011 2316 16-17 -4.900784378363667 2316 18-19 -7.108761284202085 2316 20-21 -6.58186538107978 2316 22-23 -5.000774980842905 2316 24-25 -4.943178680034638 2316 26-27 -4.586379251101945 2316 28-29 -4.552537720413142 2316 30-31 -4.172608888073853 2316 32-33 -4.380034577818833 2316 34-35 -4.444741897278572 2316 36-37 -4.35825044100423 2316 38-39 -4.343519663656878 2316 40-41 -4.439420620972935 2316 42-43 -4.580798954237913 2316 44-45 -4.585778876339777 2316 46-47 -4.281510610293807 2316 48-49 -4.627800146867749 2316 50-51 -4.934539176165448 2316 52-53 -4.729992340598159 2316 54-55 -4.995680184452723 2316 56-57 -4.990252975212414 2316 58-59 -5.241192925687766 2316 60-61 -5.204159113028627 2316 62-63 -5.173251975613063 2316 64-65 -4.839851770049158 2316 66-67 -4.787593586704272 2316 68-69 -4.771340955881534 2316 70-71 -5.041407261039939 2316 72-73 -4.6138030148219755 2316 74-75 -4.550990900293645 2316 76-77 -4.436572653538224 2316 78-79 -4.39488189377953 2316 80-81 -4.399852197653679 2316 82-83 -4.427003426783639 2316 84-85 -4.25093192216147 2316 86-87 -4.231957557158122 2316 88-89 -4.281718653586459 2316 90-91 -4.1713022943399345 2316 92-93 -4.254962788411234 2316 94-95 -4.220997067222125 2316 96-97 -4.19668738621619 2316 98-99 -4.202170974915774 2316 100-101 -4.058977658668038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1617799.0 3 430818.0 4 407977.0 5 423064.0 6 503491.0 7 656669.0 8 865271.0 9 1102480.0 10 1155307.0 11 938657.0 12 716352.0 13 572865.0 14 492267.0 15 450898.0 16 433794.0 17 436805.0 18 448046.0 19 471512.0 20 508848.0 21 559469.0 22 618181.0 23 689079.0 24 764315.0 25 849136.0 26 948483.0 27 1065999.0 28 1209879.0 29 1385314.0 30 1606400.0 31 1886887.0 32 2257232.0 33 2765485.0 34 3500559.0 35 4622397.0 36 6568694.0 37 1.0546356E7 38 1.4372478E7 39 4883411.0 40 13981.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.6271437940083 15.026301380403181 11.842484773508357 45.50407005208016 2 14.869135276979955 18.344996341303414 54.01878584383986 12.767082537876773 3 20.466079656590928 21.59908943558592 35.803612944384795 22.131217963438353 4 21.265318360596133 29.61252574505525 32.1040726758376 17.01808321851102 5 21.24295792284165 31.481824080913217 32.749020480262516 14.52619751598262 6 16.386133220665123 34.22708186033454 34.50018847498566 14.886596444014666 7 13.700826647235948 18.664648171970775 51.2903056162675 16.344219564525776 8 14.140337670087987 21.740687806005226 44.516640641087996 19.602333882818787 9 16.273562238862493 20.86230403592637 43.58960545873821 19.274528266472924 10-11 18.923414627589995 32.031365801346844 32.794062363685754 16.2511572073774 12-13 17.640664269271742 26.15501288404 38.60767615411927 17.59664669256899 14-15 18.13605430686815 27.285506949876186 37.3484461609031 17.22999258235256 16-17 19.053716508847426 27.373013056087885 35.60534866067971 17.967921774384973 18-19 19.599031600020204 27.862063763793067 34.457777071125705 18.081127565061024 20-21 19.579624851686816 27.88576739220249 34.13345196917048 18.401155786940215 22-23 19.411677115925222 28.690153344764497 34.30341697702025 17.594752562290033 24-25 19.439888564403233 28.75464260963789 33.63220161338218 18.173267212576693 26-27 19.47518663954564 29.06676734908696 33.05990460334342 18.39814140802398 28-29 19.42802720819876 29.548891814747385 32.19660090223144 18.82648007482242 30-31 19.3044621654289 29.610927301523066 31.970137478890976 19.114473054157056 32-33 19.704685343499975 29.915206581977465 31.51990528939701 18.86020278512555 34-35 19.537556662434312 30.52014750895079 30.817029759207422 19.125266069407473 36-37 19.69588578880291 29.996363258975357 31.1355751514179 19.172175800803835 38-39 19.834999142342404 29.74884073666393 31.252721674619018 19.163438446374652 40-41 20.124532349049833 29.610728835557577 31.133172126501275 19.131566688891315 42-43 19.779426269709184 29.991642830591964 31.204004850105026 19.024926049593827 44-45 20.150496318906928 29.92066901743908 30.965369187677144 18.963465475976847 46-47 19.92025726971313 29.596307331469312 31.20395866676036 19.279476732057198 48-49 20.079943018302814 29.915783245834394 30.538978486469677 19.465295249393115 50-51 19.660328238506867 29.933969357886824 31.14266822697657 19.263034176629738 52-53 19.860376557425845 29.885928364107876 30.913331300783437 19.340363777682843 54-55 19.680222314548203 30.24289070381726 30.721062187519678 19.35582479411486 56-57 19.986652529159457 29.833260277046804 30.620785919348236 19.559301274445502 58-59 19.846085505505403 29.974144451185786 30.34357945668812 19.836190586620692 60-61 19.99323985634793 30.243443877203806 30.12382270307744 19.63949356337082 62-63 19.577360028928744 30.718957378776064 30.257530512494828 19.446152079800363 64-65 19.6168216139012 30.518804727823696 30.36699325260613 19.497380405668974 66-67 19.847239696215528 30.167551723187973 30.445545026526993 19.53966355406951 68-69 19.986395934203216 30.361076536285797 30.273306574879555 19.379220954631435 70-71 19.827460540876828 30.67421340025532 29.860418068382682 19.637907990485168 72-73 19.557438244211443 30.853079521972532 30.01755646520781 19.571925768608217 74-75 19.758407478394332 30.178139117947822 30.465419936765947 19.5980334668919 76-77 19.928175650693227 30.451572152320168 29.926226140786767 19.69402605619984 78-79 19.708366641703208 30.56583030249226 29.84219332578321 19.883609730021323 80-81 20.04118310890422 30.320178949683317 30.112156824505572 19.526481116906893 82-83 19.945895708831085 30.55354083856527 29.875961560937327 19.62460189166632 84-85 19.65508285955837 30.485502896076206 30.087452928140205 19.77196131622522 86-87 19.86680979516372 30.63181372629151 29.74997856168134 19.75139791686342 88-89 19.784640664695342 30.63172159942486 29.884430842903846 19.69920689297595 90-91 19.686494269780813 30.79378227389105 29.83988640448732 19.679837051840813 92-93 19.620315571037597 30.90982308052615 29.811018984635712 19.658842363800538 94-95 19.581982353139683 30.9717973601906 29.75944677240149 19.68677351426823 96-97 19.791737238201634 30.53564522955744 29.73008146577778 19.942536066463145 98-99 19.774755403812733 30.818835820207312 29.66355096509618 19.742857810883777 100-101 20.300410980664765 31.30941806930054 28.67576924800178 19.71440170203291 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12359.0 1 16580.0 2 19589.0 3 20887.5 4 25735.0 5 31330.0 6 37611.0 7 44841.5 8 53443.5 9 62970.5 10 73533.0 11 84959.0 12 97194.5 13 110993.0 14 125041.0 15 140070.0 16 158001.5 17 178361.0 18 204732.0 19 239300.5 20 283361.0 21 341612.5 22 412605.5 23 511797.0 24 635408.5 25 778060.0 26 956930.0 27 1167875.0 28 1386913.0 29 1602477.0 30 1847527.5 31 2108212.5 32 2406806.0 33 2739821.0 34 3051684.0 35 3339756.5 36 3538665.0 37 3599862.0 38 3487074.5 39 3240624.5 40 3058185.0 41 2879019.0 42 2667140.5 43 2530587.5 44 2415542.5 45 2314149.5 46 2183301.5 47 2061986.5 48 1979333.5 49 1869857.5 50 1711048.5 51 1519150.0 52 1366350.5 53 1247902.5 54 1087309.0 55 891465.5 56 711821.5 57 552265.0 58 419464.0 59 315052.0 60 230485.5 61 163598.0 62 114097.5 63 80049.0 64 56449.0 65 39511.0 66 28135.0 67 20981.5 68 15403.5 69 10624.0 70 7931.5 71 6185.5 72 4761.5 73 4263.5 74 3692.5 75 2603.5 76 2068.0 77 1672.0 78 1311.5 79 937.5 80 690.0 81 500.5 82 335.0 83 235.0 84 183.0 85 152.5 86 127.0 87 100.0 88 66.5 89 45.5 90 31.0 91 25.5 92 15.5 93 9.5 94 7.5 95 3.5 96 2.5 97 2.5 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6750651944823802 2 0.3249001598784379 3 0.33101433549765746 4 0.333754798776975 5 0.3181283273119303 6 0.22590719538398046 7 0.11100028875885964 8 0.1276179916228065 9 0.12114447767156356 10-11 0.12031054154252827 12-13 0.11549201790915127 14-15 0.12918687634035741 16-17 0.11505606593275315 18-19 0.13198564734902213 20-21 0.13635262507838491 22-23 0.11467706569199647 24-25 0.12122787128446706 26-27 0.11391770921677735 28-29 0.1164032456794142 30-31 0.12855159328921428 32-33 0.11611509701694267 34-35 0.13781303029947595 36-37 0.13142765593910666 38-39 0.12550128544813322 40-41 0.13484747206500416 42-43 0.09869803586345711 44-45 0.35355908684943066 46-47 0.10236125285953647 48-49 0.08684868486577459 50-51 0.09064478924501727 52-53 0.23378077283640863 54-55 0.2898104327579332 56-57 0.19315926939330333 58-59 0.26957968466501975 60-61 0.07381216137870932 62-63 0.05991390389164092 64-65 0.10886866665342312 66-67 0.10475661574074105 68-69 0.08715107146215649 70-71 0.1138614354779888 72-73 0.1410009715016905 74-75 0.13145206382581012 76-77 0.12508499538046844 78-79 0.10710316284853869 80-81 0.09866345802396054 82-83 0.10527528333318982 84-85 0.10203988235127412 86-87 0.12677320754412522 88-89 0.12863973288008793 90-91 0.16245211392977757 92-93 0.16619940253561333 94-95 0.12678066550950684 96-97 0.10484000935364458 98-99 0.13139172210590433 100-101 0.12685999114129312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7.3746655E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.367446029992365 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71866092578277 26.36624438860124 2 8.620919503450192 5.9255797152746625 3 3.891217574605942 4.011936299586826 4 2.202221766894704 3.027389508793127 5 1.4868571851836418 2.554974203305259 6 1.0667467390410865 2.1996816588998938 7 0.7930776308924662 1.9079236874103729 8 0.6248411935711006 1.7179356797896652 9 0.4923963813415288 1.5230165455006681 >10 3.558788213752976 23.77900202195513 >50 0.34734544911694765 8.246885383158421 >100 0.1825233967897291 11.198083345454998 >500 0.00910566942688719 2.096158593586329 >1k 0.004677741134489225 2.992548853561694 >5k 3.859283813863622E-4 0.8858323229202782 >10k+ 2.347006342990361E-4 1.566807792201514 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008765143313957766 0.0 0.0 2.7119874114968333E-6 1.3559937057484167E-6 2 0.00883023101183369 2.7119874114968333E-6 0.0 8.1359622344905E-6 1.3559937057484167E-6 3 0.008971254357231525 2.7119874114968333E-6 0.0 1.3559937057484167E-5 2.7119874114968333E-6 4 0.010083169195945227 2.7119874114968333E-6 0.0 3.7967823760955666E-5 2.7119874114968333E-6 5 0.010342163993743174 4.06798111724525E-6 0.0 4.474779228969775E-5 2.7119874114968333E-6 6 0.010753030086584944 4.06798111724525E-6 0.0 4.8815773406943E-5 2.7119874114968333E-6 7 0.010938801224272477 6.7799685287420835E-6 0.0 5.423974822993667E-5 2.7119874114968333E-6 8 0.011513742555509805 8.1359622344905E-6 0.0 6.644369158167241E-5 2.7119874114968333E-6 9 0.011889352812002117 8.1359622344905E-6 0.0 6.779968528742084E-5 2.7119874114968333E-6 10-11 0.013494849359608242 8.1359622344905E-6 0.0 7.322366011041449E-5 2.7119874114968333E-6 12-13 0.01594174000163126 9.491955940238916E-6 0.0 8.271561605065341E-5 2.7119874114968333E-6 14-15 0.01855270588204984 1.220394335173575E-5 0.0 8.88175877265213E-5 3.3899842643710413E-6 16-17 0.021608437697953894 1.2881940204609959E-5 0.0 9.7631546813886E-5 4.06798111724525E-6 18-19 0.024924520305361644 1.4915930763232584E-5 0.0 1.057675090483765E-4 4.06798111724525E-6 20-21 0.02869486080419512 1.6271924468981E-5 0.0 1.1051348701849596E-4 4.06798111724525E-6 22-23 0.032236038366757105 1.6271924468981E-5 0.0 1.1458146813574121E-4 4.06798111724525E-6 24-25 0.0353066861134244 1.8305915027603623E-5 0.0 1.1864944925298645E-4 4.06798111724525E-6 26-27 0.03822817455245936 2.033990558622625E-5 0.0 1.2339542722310593E-4 4.06798111724525E-6 28-29 0.04114356101981846 2.033990558622625E-5 0.0 1.2881940204609958E-4 4.06798111724525E-6 30-31 0.04382368257923021 2.2373896144848877E-5 1.3559937057484167E-6 1.322093863104706E-4 4.745977970119458E-6 32-33 0.04650109215123045 2.5763880409219917E-5 1.3559937057484167E-6 1.3424337686909326E-4 5.423974822993667E-6 34-35 0.049355458901830865 2.7119874114968334E-5 1.3559937057484167E-6 1.3966735169208691E-4 5.423974822993667E-6 36-37 0.05217185782867033 2.7119874114968334E-5 1.3559937057484167E-6 1.4441332966220636E-4 5.423974822993667E-6 38-39 0.05483977544473034 2.9831861526465168E-5 2.7119874114968333E-6 1.4848131077945163E-4 6.101971675867875E-6 40-41 0.05775516191208944 3.2543848937962E-5 2.7119874114968333E-6 1.5254929189669687E-4 7.457965381616291E-6 42-43 0.06070444822209224 3.2543848937962E-5 2.7119874114968333E-6 1.5797326671969055E-4 8.1359622344905E-6 44-45 0.0635262711237547 3.2543848937962E-5 2.7119874114968333E-6 1.6000725727831317E-4 8.1359622344905E-6 46-47 0.06653725514736905 3.2543848937962E-5 2.7119874114968333E-6 1.633972415426842E-4 8.1359622344905E-6 48-49 0.06965468467688467 3.389984264371042E-5 2.7119874114968333E-6 1.6746522265992944E-4 8.813959087364708E-6 50-51 0.0730372109758741 3.389984264371042E-5 2.7119874114968333E-6 1.715332037771747E-4 1.0169952793113125E-5 52-53 0.07663466227722465 3.389984264371042E-5 2.7119874114968333E-6 1.7627918174729416E-4 1.0847949645987333E-5 54-55 0.08047822643616853 3.389984264371042E-5 3.3899842643710413E-6 1.884831250990299E-4 1.0847949645987333E-5 56-57 0.08461265124499545 3.389984264371042E-5 4.745977970119458E-6 1.959410904806462E-4 1.0847949645987333E-5 58-59 0.08900742684532607 3.389984264371042E-5 6.101971675867875E-6 2.0136506530363986E-4 1.0847949645987333E-5 60-61 0.09375476080915127 3.525583634945883E-5 8.1359622344905E-6 2.0407705271513672E-4 1.0847949645987333E-5 62-63 0.09907161212939081 3.525583634945883E-5 9.491955940238916E-6 2.0882303068525616E-4 1.0847949645987333E-5 64-65 0.10491187702004925 3.525583634945883E-5 9.491955940238916E-6 2.11535018096753E-4 1.0847949645987333E-5 66-67 0.11111487022699539 3.593383320233304E-5 9.491955940238916E-6 2.1763698977262088E-4 1.0847949645987333E-5 68-69 0.1182067173080596 3.9323817466704086E-5 1.0169952793113125E-5 2.2373896144848874E-4 1.0847949645987333E-5 70-71 0.1262897957880259 4.000181431957829E-5 1.0847949645987333E-5 2.2916293627148242E-4 1.1525946498861542E-5 72-73 0.13540885345918402 4.06798111724525E-5 1.0847949645987333E-5 2.3119692683010504E-4 1.220394335173575E-5 74-75 0.14612459371886088 4.2713801731075126E-5 1.0847949645987333E-5 2.379768953588471E-4 1.220394335173575E-5 76-77 0.15823836348916437 4.339179858394933E-5 1.1525946498861542E-5 2.4679085444621185E-4 1.220394335173575E-5 78-79 0.17255901301557336 4.474779228969775E-5 1.220394335173575E-5 2.501808387105829E-4 1.220394335173575E-5 80-81 0.1893902848881756 4.6781782848320374E-5 1.3559937057484167E-5 2.5492681668070233E-4 1.220394335173575E-5 82-83 0.20916541909595765 4.949377025981721E-5 1.3559937057484167E-5 2.5696080723932495E-4 1.220394335173575E-5 84-85 0.23386552244301792 5.084976396556563E-5 1.3559937057484167E-5 2.6509676947381543E-4 1.3559937057484167E-5 86-87 0.2640546883109478 5.1527760818439834E-5 1.3559937057484167E-5 2.7119874114968335E-4 1.3559937057484167E-5 88-89 0.30060956663051364 5.3561751377062455E-5 1.3559937057484167E-5 2.8001270023704804E-4 1.4915930763232584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 51030 0.0 74.35726 1 GCGTGTT 70910 0.0 52.974537 2 GTTTTTT 981195 0.0 42.577812 1 CGTGTTT 106145 0.0 36.02444 3 CGCGCGT 2910 0.0 23.612507 1 CGCGGGT 2605 0.0 20.51554 1 TTTAGGC 44695 0.0 20.506886 7 TTAGGCA 43970 0.0 20.079119 8 TAGGCAT 44310 0.0 19.561262 9 CGGACGT 2020 0.0 19.370243 1 GCGCGTT 3100 0.0 19.17083 2 CCGGTGT 23115 0.0 18.99064 16-17 ACTCGTC 11175 0.0 18.908466 42-43 CATTCCG 24730 0.0 18.581491 12-13 TCGTCTA 11740 0.0 18.31648 44-45 CTGGAAT 120650 0.0 17.872595 1 TCCGGTG 22690 0.0 17.84047 16-17 TTCCGGT 25705 0.0 17.381754 14-15 CTCGTAT 6905 0.0 17.345333 1 TATGGCG 81690 0.0 17.294474 7 >>END_MODULE