FastQCFastQC Report
Fri 27 May 2016
SRR1233035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73746655
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT523150.073.584461
GCGTGTT711550.054.05142
CGTGTTT1068900.036.4694563
GTTTTTT9207150.036.10491
CGCGTTT55450.025.7792741
GTGTTTT2965700.020.674661
TCGTCTA116400.019.14178744-45
ACTCGTC117050.018.77227842-43
TTTAGGC420350.017.8316737
TTAGGCA416750.017.4398738
CATTCCG221300.017.37863512-13
CTGGAAT1217750.017.2958221
CCGGTGT214150.017.2717916-17
CGCGCGT26550.017.1716671
TAGGCAT423400.016.9535589
TATGGCG845300.016.9484277
TCCGGTG205550.016.43521516-17
CGGACGT20250.016.416431
CGAGCTA135350.016.32952112-13
TCGACTC74350.016.2828163
Produced by FastQC (version 0.11.5)