FastQCFastQC Report
Fri 27 May 2016
SRR1233034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47342719
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1825480.38558833091103195No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1475530.3116698895135279No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC1208460.25525783595150076No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA997470.2106913208766062No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG627700.13258638566999076No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC572220.12086758261603014No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT518070.1094297097722672No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA475040.10034066695662326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT354100.069.903031
GCGTGTT453100.054.1155052
GTTTTTT7021450.039.507051
CGTGTTT689800.036.6685643
CCTTTAG562700.035.3563165
TAGGCAT573000.034.6941039
TTAGGCA593150.033.594938
TTTAGGC631200.031.9378687
TCGTCTA426450.031.06506744-45
ACTCGTC430750.030.86853442-43
CTAACGA420450.030.09875994-95
CCCGAAA558350.028.5551511
CTTTAGG723550.027.7427586
TCCTTTA772100.026.7749214
CGCGCGT13900.026.419861
GCCTAAC503400.025.65121892-93
CCGAAAC654100.024.3900242
GTCTATG548950.024.32260746-47
GAGGTGA537850.023.49426582-83
CGAAACC703050.023.0902353
Produced by FastQC (version 0.11.5)