##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233034_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47342719 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.196543253039607 31.0 31.0 34.0 25.0 34.0 2 30.36569570497208 33.0 31.0 34.0 25.0 34.0 3 30.377112539733936 31.0 31.0 34.0 25.0 34.0 4 33.74898142204295 37.0 35.0 37.0 28.0 37.0 5 33.629748071715106 37.0 35.0 37.0 28.0 37.0 6 33.74679791838741 37.0 35.0 37.0 28.0 37.0 7 33.785320779738065 37.0 35.0 37.0 28.0 37.0 8 33.71227252494729 37.0 35.0 37.0 28.0 37.0 9 35.24917096544455 39.0 35.0 39.0 28.0 39.0 10-11 35.1496595073046 39.0 35.0 39.0 27.5 39.0 12-13 35.07678702399835 39.0 35.0 39.0 27.0 39.0 14-15 36.02700639141575 40.0 36.0 41.0 27.0 41.0 16-17 35.782367643480725 39.0 36.0 41.0 27.0 41.0 18-19 35.40881230332377 39.0 35.0 41.0 25.0 41.0 20-21 35.1110051790646 39.0 34.0 41.0 24.5 41.0 22-23 34.81982886323027 39.0 34.0 41.0 22.5 41.0 24-25 34.43223776395268 39.0 34.0 41.0 18.0 41.0 26-27 34.09323939548128 39.0 34.0 41.0 16.5 41.0 28-29 33.86092709419583 39.0 34.0 41.0 10.5 41.0 30-31 33.602390718201036 39.0 33.0 41.0 9.5 41.0 32-33 33.41116177759034 39.0 33.0 41.0 8.5 41.0 34-35 33.20603274814022 39.0 33.0 41.0 7.5 41.0 36-37 33.05617742403008 38.5 33.0 41.0 3.5 41.0 38-39 32.9126448461906 38.0 33.0 41.0 2.0 41.0 40-41 32.763776231356715 38.0 32.0 41.0 2.0 41.0 42-43 32.62833197222999 38.0 32.0 41.0 2.0 41.0 44-45 32.37829725200194 38.0 31.0 40.0 2.0 41.0 46-47 32.257023598496744 38.0 31.0 40.0 2.0 41.0 48-49 32.175077777007274 38.0 31.0 40.0 2.0 41.0 50-51 31.64512257946148 37.5 30.5 39.5 2.0 40.5 52-53 31.696913658465625 38.0 30.5 40.0 2.0 40.5 54-55 31.98025480961497 38.0 31.0 40.0 2.0 41.0 56-57 31.886256860321012 38.0 31.0 40.5 2.0 41.0 58-59 31.7337354345871 38.0 31.0 40.0 2.0 41.0 60-61 31.630170301372004 38.0 31.0 40.0 2.0 41.0 62-63 31.407496747282302 38.0 30.0 40.0 2.0 41.0 64-65 31.14598518306479 37.0 30.0 40.0 2.0 41.0 66-67 30.914022154071887 37.0 29.5 40.0 2.0 41.0 68-69 30.605696400749608 36.5 29.0 39.5 2.0 41.0 70-71 30.25356720639556 36.0 29.0 39.0 2.0 41.0 72-73 29.92058626797502 36.0 28.5 39.0 2.0 41.0 74-75 29.535569513867593 35.0 28.0 38.5 2.0 40.0 76-77 29.14906996575334 35.0 28.0 37.5 2.0 40.0 78-79 28.771478006998287 35.0 27.5 37.0 2.0 39.0 80-81 28.3671440903088 35.0 26.0 36.5 2.0 39.0 82-83 27.999138378596296 34.0 26.0 36.0 2.0 38.5 84-85 27.667299315867346 34.0 26.0 36.0 2.0 37.0 86-87 27.277166759264503 34.0 26.0 35.0 2.0 37.0 88-89 26.978978795028652 34.0 25.0 35.0 2.0 36.5 90-91 26.68720680153584 34.0 25.0 35.0 2.0 36.0 92-93 26.366384617664227 34.0 24.0 35.0 2.0 36.0 94-95 26.004559560679226 34.0 21.5 35.0 2.0 36.0 96-97 25.662920870683408 34.0 19.5 35.0 2.0 35.0 98-99 25.239606675315798 34.0 9.5 35.0 2.0 35.0 100-101 23.57136503925767 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0749053563307172 1101 2 1.3718100027616096 1101 3 1.4274925255236575 1101 4 1.4715089418430622 1101 5 1.521733641318697 1101 6 1.264225932574611 1101 7 1.4427928112028994 1101 8 1.5607387864556088 1101 9 1.4765369825846548 1101 10-11 1.6039466332547718 1101 12-13 1.62615645500712 1101 14-15 1.7340016205593258 1101 16-17 1.705795033993681 1101 18-19 1.8426244764114728 1101 20-21 1.8612389847469757 1101 22-23 1.7886348192482444 1101 24-25 1.788874544761157 1101 26-27 1.6580661832137196 1101 28-29 1.6649952293768067 1101 30-31 1.6735384367879647 1101 32-33 1.5847192645253827 1101 34-35 1.6506624502452922 1101 36-37 1.5058161940235166 1101 38-39 1.4289678176750726 1101 40-41 1.5319909391946211 1101 42-43 1.5906868812669188 1101 44-45 1.536174479314738 1101 46-47 1.6078917300085749 1101 48-49 1.6296988517160926 1101 50-51 1.6350535289561812 1101 52-53 1.60866002261778 1101 54-55 1.6961401931962676 1101 56-57 1.7349768404408294 1101 58-59 1.8219941009428773 1101 60-61 1.8266789598537407 1101 62-63 1.8218459515829188 1101 64-65 1.8123099828919145 1101 66-67 1.7595555440668598 1101 68-69 1.783177476578345 1101 70-71 1.7970459037877973 1101 72-73 1.8197388761061184 1101 74-75 1.7720857216994368 1101 76-77 1.7455699256610977 1101 78-79 1.7604899319969753 1101 80-81 1.6976605887093257 1101 82-83 1.5786664907056043 1101 84-85 1.6430124330675788 1101 86-87 1.6876462235674552 1101 88-89 1.7145148942118382 1101 90-91 1.6235443473904354 1101 92-93 1.7437217963497744 1101 94-95 1.756191884035463 1101 96-97 1.78936355031896 1101 98-99 1.8023927388895729 1101 100-101 1.8475653726764563 1102 1 1.0992128744473746 1102 2 1.3734198923433816 1102 3 1.367114973446121 1102 4 1.384576807936753 1102 5 1.4737939380672174 1102 6 1.4308603725783655 1102 7 1.4874727200328408 1102 8 1.5704584876362304 1102 9 1.657292189811848 1102 10-11 1.6238104226132606 1102 12-13 1.5999117780796226 1102 14-15 1.8403078972110478 1102 16-17 1.8412375152743863 1102 18-19 1.770240550098947 1102 20-21 1.815556429374034 1102 22-23 1.708321169393166 1102 24-25 1.6290124488887372 1102 26-27 1.6558980777350314 1102 28-29 1.6256258096620542 1102 30-31 1.6290725979511507 1102 32-33 1.5498021698392606 1102 34-35 1.4795577653485452 1102 36-37 1.5425747248279364 1102 38-39 1.536096519817221 1102 40-41 1.593531582596853 1102 42-43 1.590706291798007 1102 44-45 1.4861295334964524 1102 46-47 1.5847001865110215 1102 48-49 1.6038422385602118 1102 50-51 1.5337467947141015 1102 52-53 1.5674357355450717 1102 54-55 1.6442467873932607 1102 56-57 1.659205854728036 1102 58-59 1.8198198194490836 1102 60-61 1.790679321104907 1102 62-63 1.7443820367313485 1102 64-65 1.7363800527617919 1102 66-67 1.7473715560606884 1102 68-69 1.6383779916175527 1102 70-71 1.712098690984071 1102 72-73 1.6665991856444506 1102 74-75 1.6390054428959964 1102 76-77 1.642273522626045 1102 78-79 1.6873736339198224 1102 80-81 1.7038319738764685 1102 82-83 1.6147213880265134 1102 84-85 1.6961279666931581 1102 86-87 1.69413325048615 1102 88-89 1.6636017333896547 1102 90-91 1.7099452553339276 1102 92-93 1.714183883361322 1102 94-95 1.7457319850899218 1102 96-97 1.8623858698944638 1102 98-99 1.8945135359263503 1102 100-101 1.8826851689438762 1103 1 1.0807133857598075 1103 2 1.3674972058947432 1103 3 1.3533887539815304 1103 4 1.3930779922694754 1103 5 1.4472392087087513 1103 6 1.344204409854413 1103 7 1.4081766126186324 1103 8 1.4655979858659833 1103 9 1.4718720923186552 1103 10-11 1.5252587020751847 1103 12-13 1.4834800222223095 1103 14-15 1.7573020079519424 1103 16-17 1.7386434524332728 1103 18-19 1.7694348893551677 1103 20-21 1.7855943041027942 1103 22-23 1.6838250550911766 1103 24-25 1.7152828979298818 1103 26-27 1.72089700106892 1103 28-29 1.7649085746889952 1103 30-31 1.7868213625051652 1103 32-33 1.689597870336847 1103 34-35 1.7242739120334747 1103 36-37 1.6784688161755739 1103 38-39 1.7280001940889775 1103 40-41 1.7287917020500316 1103 42-43 1.7758289320373422 1103 44-45 1.6789218782441608 1103 46-47 1.7203958257805994 1103 48-49 1.7592426256115061 1103 50-51 1.7337574264969255 1103 52-53 1.7283156427523956 1103 54-55 1.8576414478711705 1103 56-57 1.866730533581098 1103 58-59 2.021494192050813 1103 60-61 1.9745008262510844 1103 62-63 1.8771593016343537 1103 64-65 1.8631106297174789 1103 66-67 1.8890509040985002 1103 68-69 1.8773906920514989 1103 70-71 1.94297942522023 1103 72-73 1.9525946076947562 1103 74-75 1.8910554583361865 1103 76-77 1.8664898217571846 1103 78-79 1.8896497042843095 1103 80-81 1.9431394283781316 1103 82-83 1.903168277320173 1103 84-85 1.9199109715000198 1103 86-87 1.8660233663405528 1103 88-89 1.8751746464799375 1103 90-91 1.9806121586670677 1103 92-93 1.9490539808241643 1103 94-95 2.0420703975133137 1103 96-97 2.059418983171586 1103 98-99 2.0278068306332315 1103 100-101 2.068949224753105 1104 1 1.0230443908989528 1104 2 1.229168286526889 1104 3 1.1957174228825203 1104 4 1.249130686008705 1104 5 1.3289491698083111 1104 6 1.2118673434655562 1104 7 1.3100683503394634 1104 8 1.4209810378346504 1104 9 1.4431775151917279 1104 10-11 1.3273800653329175 1104 12-13 1.357372004210589 1104 14-15 1.6536307506234138 1104 16-17 1.6045965573633865 1104 18-19 1.5956443389681567 1104 20-21 1.5875028760104684 1104 22-23 1.5190615620685861 1104 24-25 1.5631319346125139 1104 26-27 1.5207336250034942 1104 28-29 1.4871338967403318 1104 30-31 1.4734666388162054 1104 32-33 1.5035561335286332 1104 34-35 1.5384040387436144 1104 36-37 1.4840979225713085 1104 38-39 1.536601019630119 1104 40-41 1.5151286359944152 1104 42-43 1.5160264457127397 1104 44-45 1.460125955590172 1104 46-47 1.4651909547707334 1104 48-49 1.4898407504803544 1104 50-51 1.3334504636520883 1104 52-53 1.4631593878469538 1104 54-55 1.6732543534669126 1104 56-57 1.5820046409801094 1104 58-59 1.7292902522851925 1104 60-61 1.7221087137853779 1104 62-63 1.681050730387259 1104 64-65 1.5692587877938529 1104 66-67 1.6203223380784557 1104 68-69 1.63792405064018 1104 70-71 1.6185123175123763 1104 72-73 1.634919396728428 1104 74-75 1.5991077371899571 1104 76-77 1.6007543081868008 1104 78-79 1.633206255042925 1104 80-81 1.6405077559593977 1104 82-83 1.6504664042736543 1104 84-85 1.6368996468492654 1104 86-87 1.5570386848518218 1104 88-89 1.5986081436753317 1104 90-91 1.6794186736363699 1104 92-93 1.64015783839519 1104 94-95 1.653842694669283 1104 96-97 1.6357967510917817 1104 98-99 1.7138671564008021 1104 100-101 1.7411023650864799 1105 1 0.8647342968028688 1105 2 1.1090552769584185 1105 3 1.0901612315119102 1105 4 1.1364897404594103 1105 5 1.209366521776289 1105 6 1.1138148572436108 1105 7 1.170949952066664 1105 8 1.2120687984074223 1105 9 1.2901266552385238 1105 10-11 1.2200327953335801 1105 12-13 1.1882940088581933 1105 14-15 1.469756049134844 1105 16-17 1.3865712393878482 1105 18-19 1.4092145506160847 1105 20-21 1.42013852174167 1105 22-23 1.399149909020153 1105 24-25 1.4643591388724317 1105 26-27 1.3357421522335216 1105 28-29 1.3249899390726085 1105 30-31 1.3701415987319265 1105 32-33 1.292530976758087 1105 34-35 1.2502310417890428 1105 36-37 1.1792969433185831 1105 38-39 1.2101331157863058 1105 40-41 1.241799746649221 1105 42-43 1.2657016921333195 1105 44-45 1.2254782148879286 1105 46-47 1.290111350606999 1105 48-49 1.221779823980846 1105 50-51 1.1874216184442155 1105 52-53 1.2505028176185213 1105 54-55 1.3458799366999976 1105 56-57 1.3992612800786404 1105 58-59 1.4828666509072583 1105 60-61 1.4458047210291376 1105 62-63 1.4108401901945662 1105 64-65 1.416266884222754 1105 66-67 1.4626510058705087 1105 68-69 1.4929089535975848 1105 70-71 1.503022905837053 1105 72-73 1.4876146704399744 1105 74-75 1.4805406590091685 1105 76-77 1.524367543808264 1105 78-79 1.460592345487985 1105 80-81 1.4658869021710146 1105 82-83 1.389139666137165 1105 84-85 1.397889005901451 1105 86-87 1.425902121562995 1105 88-89 1.3698830795869483 1105 90-91 1.4779606084022916 1105 92-93 1.542300442361217 1105 94-95 1.5167553457674288 1105 96-97 1.5245832838495517 1105 98-99 1.4950961415636748 1105 100-101 1.4740121571580715 1106 1 0.8573814172579048 1106 2 1.0712508407229429 1106 3 1.024781261934379 1106 4 1.131819340387601 1106 5 1.2167916535872294 1106 6 1.1144879022915148 1106 7 1.197908197941132 1106 8 1.2306010143975925 1106 9 1.28508944941823 1106 10-11 1.1433579979110249 1106 12-13 1.120953524158324 1106 14-15 1.2828911237370164 1106 16-17 1.3098017534142343 1106 18-19 1.3602498027353533 1106 20-21 1.2652881280249488 1106 22-23 1.2920997185581768 1106 24-25 1.1838898778268998 1106 26-27 1.2017210927173352 1106 28-29 1.1416978557196273 1106 30-31 1.1924983985884978 1106 32-33 1.2639787532314415 1106 34-35 1.28497507899079 1106 36-37 1.2841739730442825 1106 38-39 1.2583985696399012 1106 40-41 1.2986338573736376 1106 42-43 1.3066213129774553 1106 44-45 1.2554167477004512 1106 46-47 1.33292821800584 1106 48-49 1.3198616482529246 1106 50-51 1.1795971930247582 1106 52-53 1.1926677846515297 1106 54-55 1.2544616383423026 1106 56-57 1.3288512962316332 1106 58-59 1.4408091090392539 1106 60-61 1.3924692629207662 1106 62-63 1.450589631359552 1106 64-65 1.4222882231383274 1106 66-67 1.4405298813478176 1106 68-69 1.2880127289206378 1106 70-71 1.3798092309882435 1106 72-73 1.3724041938683342 1106 74-75 1.4157934302944035 1106 76-77 1.3682827225885994 1106 78-79 1.403268201854214 1106 80-81 1.378557400656657 1106 82-83 1.3562912283579145 1106 84-85 1.3165138752333938 1106 86-87 1.3983128363387145 1106 88-89 1.31426936694006 1106 90-91 1.3398738852069627 1106 92-93 1.40441306325069 1106 94-95 1.3417093701947138 1106 96-97 1.4119992701572954 1106 98-99 1.4276036381219726 1106 100-101 1.384312464219608 1107 1 0.6878747025387213 1107 2 0.9562294769757358 1107 3 0.8230485784837178 1107 4 0.9131516023018804 1107 5 0.9382313380803424 1107 6 0.8899142376182283 1107 7 0.9506305732381222 1107 8 1.0495383005184422 1107 9 1.0995255502056764 1107 10-11 1.045765672048148 1107 12-13 1.028952708549184 1107 14-15 1.154999968178295 1107 16-17 1.1322933527716756 1107 18-19 1.0961325152477315 1107 20-21 1.1320010092771113 1107 22-23 1.0463984058757063 1107 24-25 1.0135012103307588 1107 26-27 0.9846297509236379 1107 28-29 0.9761096817682073 1107 30-31 1.0007030673298445 1107 32-33 1.0081788156818163 1107 34-35 0.9828063498049033 1107 36-37 0.9440677709556766 1107 38-39 0.8533829606902259 1107 40-41 0.8799341395126419 1107 42-43 0.8997461570872574 1107 44-45 0.904522531397518 1107 46-47 0.9808983672638334 1107 48-49 0.9691288054809419 1107 50-51 0.9462198885530064 1107 52-53 0.8993894823594992 1107 54-55 1.067256190566031 1107 56-57 1.0299107831878374 1107 58-59 1.1040321108012172 1107 60-61 1.0855042905970471 1107 62-63 1.084971129122561 1107 64-65 1.091992460963123 1107 66-67 1.0957141325815378 1107 68-69 1.0545219932938608 1107 70-71 1.06617570193821 1107 72-73 1.0310566868759352 1107 74-75 1.0985228393871083 1107 76-77 1.0907353728996299 1107 78-79 1.0811721410308905 1107 80-81 1.1174101839600468 1107 82-83 1.1081104086783746 1107 84-85 1.0676601942169235 1107 86-87 1.0942491700452202 1107 88-89 1.0917422096439324 1107 90-91 1.0840085707259703 1107 92-93 1.0480972309013623 1107 94-95 1.0726951997458016 1107 96-97 1.0882497366443218 1107 98-99 1.1504910494619551 1107 100-101 1.1005905626517816 1108 1 0.5843667186943691 1108 2 0.8160617139467377 1108 3 0.7437259956558968 1108 4 0.7803800114594566 1108 5 0.877284621426135 1108 6 0.7854529149112324 1108 7 0.8620920963091123 1108 8 0.8770975319576522 1108 9 0.8680605753384043 1108 10-11 0.8368025265693078 1108 12-13 0.7394732717719563 1108 14-15 0.9285985882659986 1108 16-17 0.7849921072853192 1108 18-19 0.8783182164941508 1108 20-21 0.799416788558851 1108 22-23 0.6409693242659245 1108 24-25 0.7065878451842309 1108 26-27 0.6754094043102157 1108 28-29 0.7440154785818862 1108 30-31 0.7177032868868451 1108 32-33 0.7164955044331123 1108 34-35 0.7032588497172796 1108 36-37 0.690354868223487 1108 38-39 0.7028382077175337 1108 40-41 0.7374495886571992 1108 42-43 0.6454222527825664 1108 44-45 0.7072929545996018 1108 46-47 0.6637896449678777 1108 48-49 0.6744790646212877 1108 50-51 0.6184334884035287 1108 52-53 0.5198815189121966 1108 54-55 0.6751833546071389 1108 56-57 0.6344315179549014 1108 58-59 0.7952206864742557 1108 60-61 0.7037154900616649 1108 62-63 0.7545885501087675 1108 64-65 0.6533008481296321 1108 66-67 0.6435791731594591 1108 68-69 0.6729910455492565 1108 70-71 0.7716357580712696 1108 72-73 0.6317440028058208 1108 74-75 0.6752460626342547 1108 76-77 0.7130373282156661 1108 78-79 0.7282517541482108 1108 80-81 0.6510194521440376 1108 82-83 0.5597969255924546 1108 84-85 0.66113405477223 1108 86-87 0.6170362074419948 1108 88-89 0.7440516345856807 1108 90-91 0.7761769508448921 1108 92-93 0.6950121176736062 1108 94-95 0.666312275763751 1108 96-97 0.7105353350688972 1108 98-99 0.6928550499594053 1108 100-101 0.6314077046325117 1109 1 0.40417547503555795 1109 2 0.638867218056415 1109 3 0.5718945518869027 1109 4 0.5831599141271795 1109 5 0.6087289127900419 1109 6 0.6021048907906561 1109 7 0.6429503174102322 1109 8 0.725409597903159 1109 9 0.6059668814305113 1109 10-11 0.5895298787756715 1109 12-13 0.5311027017504983 1109 14-15 0.6518619158482863 1109 16-17 0.6112502722708868 1109 18-19 0.6615934004166988 1109 20-21 0.5999826255871668 1109 22-23 0.5436744331628489 1109 24-25 0.3908331456493528 1109 26-27 0.4926336747176663 1109 28-29 0.39849386718214674 1109 30-31 0.45096398325772213 1109 32-33 0.520968869415448 1109 34-35 0.41503594269126864 1109 36-37 0.3817247570112201 1109 38-39 0.37380195348940504 1109 40-41 0.4515555903911661 1109 42-43 0.43517590576009013 1109 44-45 0.46842333346333476 1109 46-47 0.40543644669532597 1109 48-49 0.4127389882507373 1109 50-51 0.3460512237119566 1109 52-53 0.42510090735975226 1109 54-55 0.5558153082194899 1109 56-57 0.528668437094268 1109 58-59 0.6426085396672683 1109 60-61 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100-101 -2.3342879572836566 2316 1 -5.44178406795745 2316 2 -6.687264364850947 2316 3 -4.361891140365415 2316 4 -4.158677004667556 2316 5 -3.961636530728697 2316 6 -3.117142404987952 2316 7 -4.7253368871703465 2316 8 -3.6098233798054196 2316 9 -3.0642735711986475 2316 10-11 -4.9193096616774525 2316 12-13 -4.362217296267083 2316 14-15 -5.885807785242758 2316 16-17 -4.219082974120816 2316 18-19 -6.487565101713756 2316 20-21 -5.972203916770624 2316 22-23 -4.281011681225216 2316 24-25 -4.283410892012043 2316 26-27 -3.9050485437364344 2316 28-29 -3.862886900865462 2316 30-31 -3.452258224258138 2316 32-33 -3.655335292528413 2316 34-35 -3.692374685182301 2316 36-37 -3.6372608344763897 2316 38-39 -3.5926903044432237 2316 40-41 -3.680328735470738 2316 42-43 -3.7975561291799558 2316 44-45 -3.842138714878086 2316 46-47 -3.5601672362456576 2316 48-49 -3.8906989436024695 2316 50-51 -4.166383057188618 2316 52-53 -3.973982070179634 2316 54-55 -4.2969077896731385 2316 56-57 -4.30569920876443 2316 58-59 -4.537977857399131 2316 60-61 -4.529879245149921 2316 62-63 -4.426144755855496 2316 64-65 -4.133909463163068 2316 66-67 -4.052114499185151 2316 68-69 -4.037634396077301 2316 70-71 -4.258083272782052 2316 72-73 -3.8244162353267974 2316 74-75 -3.7878176403966215 2316 76-77 -3.706791408050382 2316 78-79 -3.6812616898825645 2316 80-81 -3.6758814327262357 2316 82-83 -3.680714089584118 2316 84-85 -3.489017838484795 2316 86-87 -3.513268887313931 2316 88-89 -3.6064553547129066 2316 90-91 -3.482423959501812 2316 92-93 -3.600479373934448 2316 94-95 -3.5329091816711298 2316 96-97 -3.555921650251527 2316 98-99 -3.6027927479691186 2316 100-101 -3.5035648476966657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 728751.0 3 202806.0 4 199723.0 5 217480.0 6 273700.0 7 388098.0 8 555592.0 9 754635.0 10 844340.0 11 708520.0 12 526927.0 13 405141.0 14 334835.0 15 295907.0 16 278636.0 17 273540.0 18 276223.0 19 286112.0 20 308339.0 21 336078.0 22 370769.0 23 410768.0 24 457877.0 25 513146.0 26 573817.0 27 652013.0 28 746158.0 29 860635.0 30 1008007.0 31 1196378.0 32 1441146.0 33 1779139.0 34 2266566.0 35 3015423.0 36 4296211.0 37 7016859.0 38 9430424.0 39 3104513.0 40 7487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.00848413938127 15.808498303418677 11.297392669370982 46.88562488782907 2 13.727572703331617 18.432694904566844 54.89772036782314 12.942012024278396 3 19.24571020491214 21.829263029126075 36.71662350938505 22.208403256576734 4 20.463887127137415 29.093480054199883 32.79530177821018 17.647331040452517 5 20.270983632421487 32.30272191014396 32.82308468573779 14.60320977169676 6 15.868423032890961 34.8762467953986 34.175728841428445 15.079601330281992 7 12.983058203068884 19.269025673604204 51.645135857598724 16.102780265728185 8 13.369410577014829 21.37399993706253 45.36353624352607 19.893053242396576 9 14.855327460396204 21.658696004270155 44.726557300005055 18.75941923532859 10-11 17.965896370782023 32.91879194529396 33.52171945614761 15.593592227776403 12-13 16.928697854869124 26.206015092064334 39.45977326907176 17.405513783994778 14-15 17.95197460283515 27.37927434714117 37.88697549361053 16.78177555641315 16-17 18.51669785842266 27.136261003402996 36.575311504422324 17.771729633752017 18-19 18.93817592495305 28.08445285683855 35.384129338359784 17.593241879848613 20-21 19.132073777286397 27.69189649955239 34.949367121002226 18.226662602158985 22-23 18.815691442587468 29.211816771515842 34.97414195478647 16.998349831110225 24-25 18.60270439358326 29.164820847787254 34.286512510866615 17.945962247762875 26-27 18.77451804722925 29.694177721569393 33.45335091763283 18.07795331356853 28-29 18.602129986901243 29.58421213667532 33.296471154911 18.51718672151244 30-31 18.092626711810368 30.160950925657502 33.15294993335194 18.593472429180192 32-33 18.406464561417486 30.233914684010305 32.699800630255005 18.659820124317203 34-35 18.81038229947201 30.803103089908745 31.507708485759945 18.878806124859302 36-37 19.207805861661143 30.35039114118306 31.92015233652608 18.521650660629717 38-39 18.965174345256518 30.497407267028887 31.740037729629027 18.79738065808557 40-41 19.11008484630599 30.260819474153006 31.934374607035625 18.694721072505377 42-43 19.05684896088947 30.55849855285087 31.988442681271305 18.396209804988352 44-45 19.37717197536348 30.167576626954933 31.850571379703407 18.604680017978175 46-47 19.26611392774915 29.953644669149636 31.80418632567533 18.976055077425883 48-49 19.293117322941615 29.951163975838547 31.609342206609142 19.1463764946107 50-51 18.575484253091442 30.34554681697155 32.399097909411644 18.67987102052536 52-53 19.34613624292264 30.285653101303208 31.667206599235904 18.70100405653825 54-55 19.09744939708141 30.51909444293186 31.47821403469645 18.905242125290282 56-57 19.59622717349023 30.373003403665383 30.960377047765846 19.07039237507854 58-59 18.898500638436783 30.99531347134275 30.874236493595138 19.231949396625332 60-61 19.274942714824796 30.6643287849878 30.61325174083435 19.447476759353055 62-63 19.12809775515513 31.26646503799036 30.751357037105482 18.854080169749025 64-65 19.570200343748127 30.68703033523144 30.579635343987434 19.163133977033002 66-67 19.339415542068966 30.977198980133487 30.43795302818093 19.24543244961662 68-69 19.487918772484207 31.00101064866398 30.689057580611767 18.82201299824005 70-71 19.61374364577435 30.869409313604862 30.352733500529094 19.164113540091694 72-73 19.044793612624964 31.12447111514606 30.446424429893028 19.38431084233595 74-75 18.880603370851937 30.423742060733133 31.68888012073711 19.006774447677817 76-77 18.97783546171533 30.919311443046706 31.024969300672865 19.077883794565096 78-79 19.377893382693433 30.929510885906364 30.573654681449327 19.11894104995088 80-81 19.081880447361062 30.839199420273804 30.92207589278423 19.156844239580906 82-83 19.58343178170732 31.33377228031321 30.17335523042956 18.909440707549912 84-85 19.28163946666669 30.695727314762767 30.65952727411199 19.363105944458553 86-87 19.249442068995425 31.69561379357776 30.221323285356867 18.833620852069952 88-89 19.259588986123262 31.150880306692745 30.678608669501113 18.91092203768288 90-91 19.174266917792107 31.539543006575236 30.466963486859683 18.81922658877297 92-93 19.564934604502223 31.167375244876826 30.161790205764817 19.105899944856137 94-95 19.31375820529332 31.14824306681919 30.267690426340838 19.270308301546645 96-97 19.580688754809128 31.135541552722838 30.21564989427536 19.068119798192676 98-99 19.440077473025124 31.014315795043846 30.11738683546323 19.4282198964678 100-101 19.97329370494217 31.858171315330132 29.019482512634376 19.149052467093327 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10702.0 1 14627.0 2 17454.5 3 19010.0 4 24722.0 5 31094.0 6 37562.5 7 44432.5 8 52444.0 9 61406.5 10 70743.5 11 80272.0 12 89874.5 13 99711.0 14 109693.0 15 120438.0 16 131799.5 17 145624.0 18 163952.0 19 185046.0 20 214695.5 21 258158.0 22 320322.5 23 392848.0 24 470867.0 25 575496.0 26 747622.5 27 966090.5 28 1126494.5 29 1222027.5 30 1361430.0 31 1574473.0 32 1735406.0 33 1818222.0 34 1936192.0 35 2185939.5 36 2370233.5 37 2434269.5 38 2348825.5 39 2028058.5 40 1817461.0 41 1732377.5 42 1593335.5 43 1463739.0 44 1392212.0 45 1335580.0 46 1243525.0 47 1148802.0 48 1071673.5 49 1004369.5 50 918500.5 51 812870.0 52 727428.0 53 645937.5 54 558960.0 55 477989.0 56 406322.0 57 333858.0 58 266052.5 59 209954.5 60 157873.0 61 115548.5 62 84869.5 63 59323.0 64 39987.5 65 28090.0 66 19835.0 67 14141.0 68 10217.0 69 6989.5 70 5641.5 71 6309.5 72 6049.0 73 7017.0 74 7170.0 75 4595.0 76 3525.5 77 3144.5 78 2652.5 79 1867.5 80 1269.5 81 846.5 82 553.5 83 347.0 84 236.5 85 185.5 86 162.5 87 121.5 88 73.0 89 55.5 90 48.0 91 29.5 92 22.0 93 17.5 94 11.5 95 6.5 96 5.5 97 3.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6209761631984002 2 0.30566474223839996 3 0.3115917359964053 4 0.3137187790164735 5 0.29871119147170233 6 0.2114453967039789 7 0.10631624263067779 8 0.12186668028086854 9 0.11514336555109984 10-11 0.11430585556355562 12-13 0.10991764118997897 14-15 0.12266088899541237 16-17 0.11021441332932315 18-19 0.1269529956655003 20-21 0.13070647674460775 22-23 0.1106991763612056 24-25 0.1175830226396587 26-27 0.11073825311976694 28-29 0.11302265930269023 30-31 0.12440455733013561 32-33 0.1128948677409086 34-35 0.13335735955511976 36-37 0.12791618495760668 38-39 0.12222576400818888 40-41 0.13069485932990033 42-43 0.0959313300108513 44-45 0.32612406566678187 46-47 0.09921694611583251 48-49 0.08434876754755045 50-51 0.08809063121195046 52-53 0.21924279422987936 54-55 0.26995492168500085 56-57 0.1807595799472354 58-59 0.25668255344607477 60-61 0.07138373273406624 62-63 0.05848840240882658 64-65 0.1036685282059951 66-67 0.10035334049994045 68-69 0.08434348690450162 70-71 0.10951314393243869 72-73 0.1366757156470037 74-75 0.12776621469501995 76-77 0.12055391241892971 78-79 0.10447012982080729 80-81 0.09551310308138407 82-83 0.1023863880737395 84-85 0.09968164270412945 86-87 0.12245705617372757 88-89 0.1244415218314774 90-91 0.1578035684853673 92-93 0.1612782316114966 94-95 0.12331252034763782 96-97 0.1022321932967137 98-99 0.1282425286980243 100-101 0.12485658037511534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.7342719E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.439106825680096 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96435337263718 24.511296342774124 2 8.242184584231245 5.182538432412396 3 3.5656707069929254 3.3630450678704653 4 2.114236774404571 2.658788632211465 5 1.3992090073413697 2.199494072662956 6 0.9896034937693705 1.866734997340889 7 0.7479128045389438 1.6459597390735694 8 0.6016918218816955 1.5133322771421365 9 0.46473805536392704 1.3149854431690802 >10 3.331258543602776 20.63757624263434 >50 0.35112721722224094 7.644416573386192 >100 0.2026399206231143 11.82387630979882 >500 0.014525063912212957 3.1198102040690325 >1k 0.009260315453358087 5.754216318983101 >5k 0.0010269298625135072 2.217385696836457 >10k+ 5.613881625739107E-4 4.546543649634897 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 182548 0.38558833091103195 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 147553 0.3116698895135279 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 120846 0.25525783595150076 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 99747 0.2106913208766062 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 62770 0.13258638566999076 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 57222 0.12086758261603014 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 51807 0.1094297097722672 No Hit CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA 47504 0.10034066695662326 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01495055659984379 2.1122572195314763E-6 0.0 6.3367716585944294E-6 0.0 2 0.015039271403064111 2.1122572195314763E-6 0.0 8.449028878125905E-6 0.0 3 0.015163894579016469 4.224514439062953E-6 0.0 1.0561286097657381E-5 2.1122572195314763E-6 4 0.015991899409072807 4.224514439062953E-6 0.0 1.689805775625181E-5 2.1122572195314763E-6 5 0.016262268333172836 4.224514439062953E-6 0.0 1.689805775625181E-5 2.1122572195314763E-6 6 0.016560096601126777 4.224514439062953E-6 0.0 1.901031497578329E-5 2.1122572195314763E-6 7 0.016760761036982267 4.224514439062953E-6 0.0 3.1683858292972144E-5 2.1122572195314763E-6 8 0.017204335053083875 4.224514439062953E-6 0.0 3.1683858292972144E-5 2.1122572195314763E-6 9 0.017495826549379218 4.224514439062953E-6 0.0 3.59083727320351E-5 2.1122572195314763E-6 10-11 0.018630108676267625 4.224514439062953E-6 0.0 3.802062995156658E-5 2.1122572195314763E-6 12-13 0.02034314928130765 4.224514439062953E-6 0.0 4.3301273000395264E-5 2.1122572195314763E-6 14-15 0.02256841226208406 4.224514439062953E-6 0.0 5.0694173268755436E-5 2.1122572195314763E-6 16-17 0.025122131240497614 4.224514439062953E-6 0.0 5.597481631758412E-5 3.1683858292972147E-6 18-19 0.027934601728303775 4.224514439062953E-6 0.0 5.914320214688133E-5 4.224514439062953E-6 20-21 0.030983645024697463 5.280643048828691E-6 0.0 6.231158797617855E-5 5.280643048828691E-6 22-23 0.03406437217938412 6.3367716585944294E-6 0.0 6.336771658594429E-5 6.3367716585944294E-6 24-25 0.03671208660406683 8.449028878125905E-6 0.0 6.759223102500724E-5 6.3367716585944294E-6 26-27 0.0395826441654101 1.0561286097657381E-5 0.0 9.188318904961922E-5 6.3367716585944294E-6 28-29 0.04202652576840802 1.161741470742312E-5 0.0 1.0983737541563677E-4 6.3367716585944294E-6 30-31 0.0444376673845032 1.2673543317188859E-5 0.0 1.1406188985469972E-4 6.3367716585944294E-6 32-33 0.046756925811548763 1.2673543317188859E-5 0.0 1.1511801846446546E-4 6.3367716585944294E-6 34-35 0.049441604737573266 1.3729671926954598E-5 0.0 1.161741470742312E-4 6.3367716585944294E-6 36-37 0.051872812797254 1.4785800536720335E-5 0.0 1.2251091873282562E-4 6.3367716585944294E-6 38-39 0.054283954413349184 1.4785800536720335E-5 0.0 1.2567930456212282E-4 8.449028878125905E-6 40-41 0.05685245919229945 1.689805775625181E-5 0.0 1.2990381900118578E-4 8.449028878125905E-6 42-43 0.05953291360388489 1.901031497578329E-5 0.0 1.3940897648907744E-4 8.449028878125905E-6 44-45 0.06236439440666684 1.901031497578329E-5 0.0 1.4257736231837464E-4 8.449028878125905E-6 46-47 0.06527086034074216 1.901031497578329E-5 0.0 1.5419477702579778E-4 8.449028878125905E-6 48-49 0.06837271006762413 1.901031497578329E-5 0.0 1.5736316285509501E-4 8.449028878125905E-6 50-51 0.07176499516219167 1.901031497578329E-5 0.0 1.6369993451368942E-4 8.449028878125905E-6 52-53 0.07563042587393429 1.901031497578329E-5 0.0 1.6686832034298665E-4 8.449028878125905E-6 54-55 0.07949163207123781 1.901031497578329E-5 0.0 1.6792444895275238E-4 8.449028878125905E-6 56-57 0.08360630913488513 2.323482941484624E-5 0.0 1.7214896339181533E-4 8.449028878125905E-6 58-59 0.08824588211758602 2.323482941484624E-5 0.0 1.795418636601755E-4 9.505157487891642E-6 60-61 0.09335015168858384 2.323482941484624E-5 0.0 1.8376637809923844E-4 1.0561286097657381E-5 62-63 0.09830339486838514 2.429095802461198E-5 0.0 1.911592783675986E-4 1.0561286097657381E-5 64-65 0.10369704367845878 2.5347086634377718E-5 0.0 2.0383282168478747E-4 1.0561286097657381E-5 66-67 0.10971063998246489 2.7459343853909195E-5 0.0 2.0911346473361618E-4 1.0561286097657381E-5 68-69 0.11635157668067185 3.0627729683206405E-5 0.0 2.1016959334338193E-4 1.0561286097657381E-5 70-71 0.12383213562364256 3.1683858292972144E-5 0.0 2.1650636500197633E-4 1.0561286097657381E-5 72-73 0.132166046483304 3.379611551250362E-5 0.0 2.207308794410393E-4 1.0561286097657381E-5 74-75 0.1419141135514418 3.59083727320351E-5 0.0 2.291799083191652E-4 1.0561286097657381E-5 76-77 0.15331819027969223 3.59083727320351E-5 0.0 2.6931279549026325E-4 1.0561286097657381E-5 78-79 0.1660741116284428 3.802062995156658E-5 0.0 2.851547246367493E-4 1.0561286097657381E-5 80-81 0.18017870921186424 4.0132887171098054E-5 0.0 3.031089110027669E-4 1.0561286097657381E-5 82-83 0.19697326636435902 4.0132887171098054E-5 0.0 3.126140684906585E-4 1.0561286097657381E-5 84-85 0.21699957706273693 4.0132887171098054E-5 0.0 3.178947115394872E-4 1.0561286097657381E-5 86-87 0.24098214553329733 4.0132887171098054E-5 0.0 3.242314831980816E-4 1.2673543317188859E-5 88-89 0.26940362255070305 4.0132887171098054E-5 0.0 3.3056825485667607E-4 1.2673543317188859E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 35410 0.0 69.90303 1 GCGTGTT 45310 0.0 54.115505 2 GTTTTTT 702145 0.0 39.50705 1 CGTGTTT 68980 0.0 36.668564 3 CCTTTAG 56270 0.0 35.356316 5 TAGGCAT 57300 0.0 34.694103 9 TTAGGCA 59315 0.0 33.59493 8 TTTAGGC 63120 0.0 31.937868 7 TCGTCTA 42645 0.0 31.065067 44-45 ACTCGTC 43075 0.0 30.868534 42-43 CTAACGA 42045 0.0 30.098759 94-95 CCCGAAA 55835 0.0 28.555151 1 CTTTAGG 72355 0.0 27.742758 6 TCCTTTA 77210 0.0 26.774921 4 CGCGCGT 1390 0.0 26.41986 1 GCCTAAC 50340 0.0 25.651218 92-93 CCGAAAC 65410 0.0 24.390024 2 GTCTATG 54895 0.0 24.322607 46-47 GAGGTGA 53785 0.0 23.494265 82-83 CGAAACC 70305 0.0 23.090235 3 >>END_MODULE