FastQCFastQC Report
Fri 27 May 2016
SRR1233034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47342719
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1392350.2941001339614651No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1352690.28572292182880327No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA993810.2099182347342577No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC795520.16803428632816803No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC585360.1236430886024945No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG510070.107739903996642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT364600.070.205751
GCGTGTT458850.055.9804532
CGTGTTT686700.038.2672733
CCTTTAG524450.033.2088245
GTTTTTT6667900.033.0026471
TAGGCAT548200.031.4812839
TCGTCTA425300.031.09429744-45
TTAGGCA558950.030.942898
ACTCGTC429600.030.8337842-43
TTTAGGC594600.029.5249737
CTAACGA433250.029.51124494-95
CCCGAAA496150.028.7915741
TCGACTC39450.026.1149793
CTTTAGG685750.025.6324146
CGCGTTT36150.024.9600731
GCCTAAC517500.024.88201392-93
CCGAAAC588400.024.623032
GTCTATG554800.024.1087746-47
CCAAACG668350.024.0736668
TCCTTTA718100.023.712964
Produced by FastQC (version 0.11.5)