##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47342719 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.484604232384708 31.0 30.0 34.0 25.0 34.0 2 30.16465190771996 31.0 30.0 34.0 26.0 34.0 3 27.93245863212884 31.0 27.0 34.0 16.0 34.0 4 31.38143791445523 35.0 30.0 37.0 19.0 37.0 5 32.57366960693576 35.0 32.0 37.0 23.0 37.0 6 33.142194431207045 35.0 33.0 37.0 28.0 37.0 7 33.534517335178826 36.0 35.0 37.0 29.0 37.0 8 33.59707098783236 37.0 35.0 37.0 29.0 37.0 9 35.11338731938907 39.0 35.0 39.0 29.0 39.0 10-11 35.000955722462834 39.0 35.0 39.0 28.5 39.0 12-13 34.93851261479088 39.0 35.0 39.0 28.0 39.0 14-15 36.028498384302765 40.0 36.0 41.0 27.0 41.0 16-17 35.83169485892857 40.0 36.0 41.0 27.0 41.0 18-19 35.529562307141674 40.0 35.5 41.0 27.0 41.0 20-21 35.196110018522596 39.5 35.0 41.0 25.0 41.0 22-23 34.928726474286364 39.0 35.0 41.0 23.0 41.0 24-25 34.628863532320565 39.0 34.5 41.0 18.5 41.0 26-27 34.2278951173041 39.0 34.0 41.0 13.0 41.0 28-29 33.88356595023619 39.0 34.0 41.0 9.0 41.0 30-31 33.599457901858145 39.0 33.5 41.0 3.5 41.0 32-33 33.34899971630273 39.0 33.0 41.0 2.0 41.0 34-35 33.3277552246207 39.0 33.0 41.0 2.0 41.0 36-37 33.24158337842827 39.0 33.0 41.0 2.0 41.0 38-39 33.157250442248575 39.0 33.0 41.0 2.0 41.0 40-41 33.044344104950966 39.0 33.0 41.0 2.0 41.0 42-43 32.94531698739145 39.0 33.0 41.0 2.0 41.0 44-45 32.81470169679101 39.0 33.0 41.0 2.0 41.0 46-47 32.69498365736028 39.0 33.0 41.0 2.0 41.0 48-49 32.7000051053257 39.0 33.0 41.0 2.0 41.0 50-51 32.67925235768567 39.0 33.0 41.0 2.0 41.0 52-53 32.561977101484175 39.0 33.0 41.0 2.0 41.0 54-55 32.43519500855031 39.0 32.0 41.0 2.0 41.0 56-57 32.3032180513333 39.0 32.0 41.0 2.0 41.0 58-59 32.12222115928745 38.0 32.0 40.5 2.0 41.0 60-61 31.925890758407856 38.0 31.5 40.0 2.0 41.0 62-63 31.682825293578937 38.0 31.0 40.0 2.0 41.0 64-65 31.2498934524652 37.5 31.0 40.0 2.0 41.0 66-67 31.07839902477929 37.0 30.5 40.0 2.0 41.0 68-69 30.70033618052229 36.5 30.0 40.0 2.0 41.0 70-71 30.457557084965906 36.0 29.5 39.0 2.0 41.0 72-73 30.18069242072894 36.0 29.0 39.0 2.0 41.0 74-75 29.833988052101528 35.0 29.0 38.5 2.0 40.0 76-77 28.688177077450916 34.0 28.0 36.5 2.0 39.0 78-79 29.13428522134523 35.0 29.0 37.0 2.0 39.0 80-81 28.960747976895874 35.0 29.0 37.0 2.0 39.0 82-83 28.65626028999306 35.0 29.0 36.0 2.0 39.0 84-85 28.344705655794716 35.0 29.0 36.0 2.0 37.0 86-87 27.985956784611375 34.0 28.5 35.5 2.0 37.0 88-89 27.718432817515193 34.0 28.0 35.0 2.0 36.5 90-91 27.46051408665396 34.0 27.0 35.0 2.0 36.0 92-93 27.21563845963304 34.0 26.5 35.0 2.0 36.0 94-95 26.964059584325945 34.0 26.0 35.0 2.0 36.0 96-97 26.69155966728485 34.0 25.5 35.0 2.0 35.5 98-99 26.383797548679027 34.0 25.0 35.0 2.0 35.0 100-101 25.48106161794383 33.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.2235352007980325 1101 2 1.8400906983161995 1101 3 4.071613246445377 1101 4 4.020606085595805 1101 5 2.6307118349926597 1101 6 2.012364631219583 1101 7 1.6815249754727546 1101 8 1.5532874286181695 1101 9 1.7228922708671988 1101 10-11 1.6626130998699296 1101 12-13 1.6446855265132498 1101 14-15 1.8808838411332403 1101 16-17 1.926466325254495 1101 18-19 1.8799131381784804 1101 20-21 1.885369427669879 1101 22-23 1.8914452643954007 1101 24-25 1.8768072552378783 1101 26-27 1.8519812238187185 1101 28-29 1.8826539427753062 1101 30-31 1.823205267214803 1101 32-33 1.795653283341224 1101 34-35 1.8197573588138383 1101 36-37 1.82869589867299 1101 38-39 1.8793476669576918 1101 40-41 1.916074785589693 1101 42-43 1.8693800368411289 1101 44-45 1.9247763842410706 1101 46-47 1.8686201225054475 1101 48-49 1.974606387168734 1101 50-51 1.8869024483268362 1101 52-53 1.8097177113848062 1101 54-55 1.7884587014234228 1101 56-57 1.831958855408132 1101 58-59 1.8662794882731717 1101 60-61 1.8289770373221472 1101 62-63 1.8222320740135771 1101 64-65 1.4024727845389613 1101 66-67 1.7494316560776042 1101 68-69 1.6345579587977568 1101 70-71 1.7044305919046323 1101 72-73 1.725640770401828 1101 74-75 1.80657659797971 1101 76-77 1.6814622991591825 1101 78-79 1.8588706084081181 1101 80-81 1.807673903761735 1101 82-83 1.801147852420307 1101 84-85 1.8437661600878457 1101 86-87 1.799021272311009 1101 88-89 1.8540831671427078 1101 90-91 1.9241500273111143 1101 92-93 1.8986691695775697 1101 94-95 1.8800812523225687 1101 96-97 2.0162760812372724 1101 98-99 2.012055871476285 1101 100-101 2.0833760256380565 1102 1 2.1651107324513816 1102 2 1.8153701269796656 1102 3 4.044528050990845 1102 4 4.020298617574415 1102 5 2.609652083445795 1102 6 1.96103873997059 1102 7 1.6310690184817602 1102 8 1.4488669839079549 1102 9 1.600195401519457 1102 10-11 1.6502410301764456 1102 12-13 1.640759230894595 1102 14-15 1.8470693632515918 1102 16-17 1.8080938052577409 1102 18-19 1.7860249908824386 1102 20-21 1.8142320603208546 1102 22-23 1.8515691814696638 1102 24-25 1.8758817811760693 1102 26-27 1.8171583237532118 1102 28-29 1.8494744833758432 1102 30-31 1.7802999056828241 1102 32-33 1.7692209528082117 1102 34-35 1.7865971921883315 1102 36-37 1.7196362054228302 1102 38-39 1.7523042226397862 1102 40-41 1.8103151929484511 1102 42-43 1.7748489176078195 1102 44-45 1.8483893555684787 1102 46-47 1.83303005552159 1102 48-49 1.8027166653628868 1102 50-51 1.811289236386969 1102 52-53 1.8957759599350865 1102 54-55 1.9311605883331922 1102 56-57 1.8997315150454384 1102 58-59 1.9240634118648146 1102 60-61 1.8927856913517758 1102 62-63 1.8717014673147148 1102 64-65 1.8134841151853855 1102 66-67 1.8406659663601062 1102 68-69 1.6731076046355682 1102 70-71 1.7173466330385025 1102 72-73 1.7830309335475434 1102 74-75 1.8232637169817139 1102 76-77 1.7539707598704055 1102 78-79 1.8217947346079661 1102 80-81 1.8094269763153896 1102 82-83 1.7943084310572281 1102 84-85 1.755707643217562 1102 86-87 1.7412616953522502 1102 88-89 1.7201750224694585 1102 90-91 1.7377302061872406 1102 92-93 1.7893116782444203 1102 94-95 1.8222633273988045 1102 96-97 1.8810708944263936 1102 98-99 1.8074991069567652 1102 100-101 1.9071390153923815 1103 1 2.1328141569869565 1103 2 1.7576646448754651 1103 3 3.9770922250231635 1103 4 4.012520929490709 1103 5 2.582209151189531 1103 6 1.9455829152361375 1103 7 1.5635799785246647 1103 8 1.4887941154511566 1103 9 1.6355239177638552 1103 10-11 1.6382156992654302 1103 12-13 1.6350012421028453 1103 14-15 1.7661762404426185 1103 16-17 1.8177514620757478 1103 18-19 1.7734643706688047 1103 20-21 1.7845537459904222 1103 22-23 1.8023535820197623 1103 24-25 1.769365983093131 1103 26-27 1.7664837703471008 1103 28-29 1.7877808688095485 1103 30-31 1.708799589269546 1103 32-33 1.7497600863674805 1103 34-35 1.7317777653595599 1103 36-37 1.7150115260145284 1103 38-39 1.766468026682908 1103 40-41 1.7791393382251641 1103 42-43 1.7736285678787098 1103 44-45 1.7624167538043167 1103 46-47 1.7099662567094924 1103 48-49 1.7138344046189644 1103 50-51 1.686256235598357 1103 52-53 1.726932047117998 1103 54-55 1.7899927876434063 1103 56-57 1.7841201565113067 1103 58-59 1.7996348019092139 1103 60-61 1.8082318396914374 1103 62-63 1.8143802849885233 1103 64-65 1.6675765209452855 1103 66-67 1.7706246308787073 1103 68-69 1.6607426276591823 1103 70-71 1.6493466459418613 1103 72-73 1.660374101937517 1103 74-75 1.7399360788850373 1103 76-77 1.6310593814169927 1103 78-79 1.7287626189357859 1103 80-81 1.7562095171633452 1103 82-83 1.7650045130648628 1103 84-85 1.7561288546931948 1103 86-87 1.7325530412024222 1103 88-89 1.662383836438842 1103 90-91 1.6549035758226225 1103 92-93 1.567728992770956 1103 94-95 1.629060516480866 1103 96-97 1.6859092716577848 1103 98-99 1.6787732807748519 1103 100-101 1.7322766019468858 1104 1 2.080547574522857 1104 2 1.6658962187675534 1104 3 3.9003677970152353 1104 4 3.9326554733770003 1104 5 2.5027412930667623 1104 6 1.894399996668227 1104 7 1.5219049392571478 1104 8 1.4328964149450059 1104 9 1.499529340412984 1104 10-11 1.4975353371706461 1104 12-13 1.4776484162658576 1104 14-15 1.6445113875020212 1104 16-17 1.6630069244439554 1104 18-19 1.6049575317639153 1104 20-21 1.6244978100333682 1104 22-23 1.610396827188751 1104 24-25 1.6613101925327456 1104 26-27 1.6762178268121417 1104 28-29 1.6682140483042218 1104 30-31 1.6829496378139197 1104 32-33 1.6515622649778692 1104 34-35 1.6295564769072612 1104 36-37 1.6472246503001458 1104 38-39 1.6497272623437738 1104 40-41 1.6584821300057229 1104 42-43 1.6095580359927553 1104 44-45 1.70104173623821 1104 46-47 1.7116955603824735 1104 48-49 1.7453962104349188 1104 50-51 1.7290189160735139 1104 52-53 1.6592837995132044 1104 54-55 1.7445793598664565 1104 56-57 1.7380288128590422 1104 58-59 1.7210249970687101 1104 60-61 1.7768258389988887 1104 62-63 1.7581183250076649 1104 64-65 1.6724884642642444 1104 66-67 1.789410820143722 1104 68-69 1.6589786394526769 1104 70-71 1.6764634776584444 1104 72-73 1.7123919286451432 1104 74-75 1.7250443746341482 1104 76-77 1.6976367325114907 1104 78-79 1.7455755680191025 1104 80-81 1.6471903508712877 1104 82-83 1.637414499308811 1104 84-85 1.7450547752097236 1104 86-87 1.7714070143165053 1104 88-89 1.7235439959660361 1104 90-91 1.80013233205759 1104 92-93 1.7534998122000864 1104 94-95 1.787838786529715 1104 96-97 1.7318095121960688 1104 98-99 1.817987298926628 1104 100-101 1.9139030576634077 1105 1 1.7858219204624852 1105 2 1.442917572720976 1105 3 3.0717075844453277 1105 4 3.4877086415485117 1105 5 2.072186245393759 1105 6 1.55952584432179 1105 7 1.2792997045741075 1105 8 1.1873797380005087 1105 9 1.2294654651776682 1105 10-11 1.2415276343262889 1105 12-13 1.2273291837833469 1105 14-15 1.3659141890402466 1105 16-17 1.4035974120889776 1105 18-19 1.3760901352862476 1105 20-21 1.3720806978989586 1105 22-23 1.380442817616455 1105 24-25 1.4587260652515752 1105 26-27 1.4196867597797151 1105 28-29 1.388847018569237 1105 30-31 1.353418637606758 1105 32-33 1.381679041103716 1105 34-35 1.3247518409001628 1105 36-37 1.291078115580774 1105 38-39 1.3429994001992753 1105 40-41 1.366606169911151 1105 42-43 1.3290627427977029 1105 44-45 1.3834253133253966 1105 46-47 1.4713110988960239 1105 48-49 1.3884091817623698 1105 50-51 1.3997640928261958 1105 52-53 1.417252571553469 1105 54-55 1.3954897998694165 1105 56-57 1.4414116323389834 1105 58-59 1.4188107156984628 1105 60-61 1.4028312616076448 1105 62-63 1.4137063493786357 1105 64-65 1.2679301290653378 1105 66-67 1.376233826018705 1105 68-69 1.3101179854211793 1105 70-71 1.3439357906581009 1105 72-73 1.4365759520089192 1105 74-75 1.3956634586002465 1105 76-77 1.326014854249749 1105 78-79 1.4113818396358973 1105 80-81 1.4391816409172868 1105 82-83 1.4557243547623635 1105 84-85 1.4875744550554906 1105 86-87 1.4448331440540443 1105 88-89 1.3891447307200693 1105 90-91 1.43951342781779 1105 92-93 1.51012989848698 1105 94-95 1.5272134568739766 1105 96-97 1.4919275156932912 1105 98-99 1.5379419111440846 1105 100-101 1.655553303172141 1106 1 1.841473482507606 1106 2 1.4676420076234713 1106 3 3.6926675260385764 1106 4 3.759731168149127 1106 5 2.2588367132919345 1106 6 1.6365072552638296 1106 7 1.3039953209066866 1106 8 1.2049627877115583 1106 9 1.2360335452388256 1106 10-11 1.2460192087709245 1106 12-13 1.2669732362816362 1106 14-15 1.3926506311812474 1106 16-17 1.3581133953491502 1106 18-19 1.2294197094790604 1106 20-21 1.243209081620499 1106 22-23 1.1529059945801805 1106 24-25 1.1930112203628624 1106 26-27 1.1958587399044873 1106 28-29 1.2782957090225153 1106 30-31 1.184289152464956 1106 32-33 1.2176382007260855 1106 34-35 1.2023456676816338 1106 36-37 1.1368243655968868 1106 38-39 1.1643050231610843 1106 40-41 1.2199756229108587 1106 42-43 1.3072300412353783 1106 44-45 1.2665161395194033 1106 46-47 1.1904052076339866 1106 48-49 1.2161078219025825 1106 50-51 1.1116174462441322 1106 52-53 1.3392548985690027 1106 54-55 1.4544135268026679 1106 56-57 1.357333924687346 1106 58-59 1.3479618047536022 1106 60-61 1.3738561041575181 1106 62-63 1.3860577400030891 1106 64-65 1.325344359833874 1106 66-67 1.3869839348760564 1106 68-69 1.3447021182709022 1106 70-71 1.3279903972100264 1106 72-73 1.2685110399537898 1106 74-75 1.284092222152001 1106 76-77 1.2086938498590278 1106 78-79 1.3357028317196473 1106 80-81 1.3329975797605549 1106 82-83 1.357152245818238 1106 84-85 1.4035649432694086 1106 86-87 1.4324166389137503 1106 88-89 1.3753917870583585 1106 90-91 1.3692630949488453 1106 92-93 1.4089649504767543 1106 94-95 1.3796302148887385 1106 96-97 1.387616357049243 1106 98-99 1.4246392885941184 1106 100-101 1.4017069586308608 1107 1 1.5748909324108773 1107 2 1.2737975864619528 1107 3 2.24307404030694 1107 4 3.036751033298632 1107 5 1.8771128778458603 1107 6 1.3734168160196774 1107 7 1.074775867201815 1107 8 0.981104459903662 1107 9 1.067360189889719 1107 10-11 0.9496612366677653 1107 12-13 0.9834247305267283 1107 14-15 1.042330576757223 1107 16-17 1.0750411987796298 1107 18-19 1.0782150007695037 1107 20-21 1.0632904771535223 1107 22-23 1.0670329002019372 1107 24-25 1.0779136896558512 1107 26-27 1.0270998570969283 1107 28-29 1.016587102687339 1107 30-31 0.9931647853741765 1107 32-33 1.0830181286520926 1107 34-35 1.0640675115823157 1107 36-37 1.0476693141667681 1107 38-39 1.063984240184027 1107 40-41 1.0813851528295118 1107 42-43 1.0485697929370232 1107 44-45 1.099014313055477 1107 46-47 1.0690188182024372 1107 48-49 1.1453021171943192 1107 50-51 1.1219930888397585 1107 52-53 1.067271728560435 1107 54-55 0.9423106776315677 1107 56-57 0.9440436395686049 1107 58-59 1.0284059589870154 1107 60-61 1.1329727531295504 1107 62-63 1.1378481048750544 1107 64-65 1.021651407083322 1107 66-67 1.1339471604080842 1107 68-69 1.0569149806844251 1107 70-71 1.1313911905552914 1107 72-73 1.1944299577332806 1107 74-75 1.1055692460483009 1107 76-77 0.9879745467571368 1107 78-79 1.0916384816507936 1107 80-81 1.1396418016973406 1107 82-83 1.185219902893028 1107 84-85 1.1901185846597713 1107 86-87 1.165359927879571 1107 88-89 1.1680174878813396 1107 90-91 1.0999742362898708 1107 92-93 1.139345112955187 1107 94-95 1.0763965747098787 1107 96-97 1.0398151138816871 1107 98-99 1.0995475099077332 1107 100-101 1.145277251978051 1108 1 1.3223818881710976 1108 2 1.0625649947623828 1108 3 1.4329336368549797 1108 4 2.609484539865573 1108 5 1.5519321339782124 1108 6 1.1065841469343738 1108 7 0.8768255307153723 1108 8 0.7839878662877595 1108 9 0.8007459681164946 1108 10-11 0.8434924520464193 1108 12-13 0.8182755679605194 1108 14-15 0.8147082509191677 1108 16-17 0.8480646606505218 1108 18-19 0.8358546822333821 1108 20-21 0.7811850130347793 1108 22-23 0.8248825717901411 1108 24-25 0.8787533691464589 1108 26-27 0.8506523083902309 1108 28-29 0.8538996115217543 1108 30-31 0.86325217654705 1108 32-33 0.8581489470302088 1108 34-35 0.8486108164515045 1108 36-37 0.8478411894804339 1108 38-39 0.8530914629422526 1108 40-41 0.7865917143231442 1108 42-43 0.8017579731511901 1108 44-45 0.86063026251027 1108 46-47 0.8367861300528432 1108 48-49 0.8314778161276308 1108 50-51 0.7651938676083603 1108 52-53 0.7846965786587035 1108 54-55 0.8250804307959569 1108 56-57 0.7555095609886848 1108 58-59 0.8128699227794058 1108 60-61 0.7117925308284327 1108 62-63 0.78924014603313 1108 64-65 0.7790073516248945 1108 66-67 0.840618900947053 1108 68-69 0.7434099673770227 1108 70-71 0.6451469452405192 1108 72-73 0.663882672945082 1108 74-75 0.6972959408414638 1108 76-77 0.6112688685935801 1108 78-79 0.7353888417436849 1108 80-81 0.7974669581415839 1108 82-83 0.8285333704377074 1108 84-85 0.8700136352871475 1108 86-87 0.8550155039496588 1108 88-89 0.8187244562562341 1108 90-91 0.7695788024941237 1108 92-93 0.8407843812592226 1108 94-95 0.8043261048310519 1108 96-97 0.7592405430424485 1108 98-99 0.7157661353202975 1108 100-101 0.7148613299830302 1109 1 0.974239830880709 1109 2 0.7505594430852369 1109 3 -1.11620054476926 1109 4 1.684879729311529 1109 5 1.0144937713457765 1109 6 0.7771231994643912 1109 7 0.6219122703528797 1109 8 0.5564828006116969 1109 9 0.5737832526493065 1109 10-11 0.6131691622861482 1109 12-13 0.6009320703419192 1109 14-15 0.5762870674277565 1109 16-17 0.6092753710545367 1109 18-19 0.6025638146112939 1109 20-21 0.5478067983673043 1109 22-23 0.5756900629085919 1109 24-25 0.5641640267342112 1109 26-27 0.5369160956400734 1109 28-29 0.4695030626088794 1109 30-31 0.5051609497984657 1109 32-33 0.4995691483490603 1109 34-35 0.5083138677765291 1109 36-37 0.5697250234365754 1109 38-39 0.5592577900285463 1109 40-41 0.5428661852341037 1109 42-43 0.47462833929295556 1109 44-45 0.48182175969223806 1109 46-47 0.490809879984198 1109 48-49 0.43980942382040666 1109 50-51 0.4523927708733453 1109 52-53 0.4984319261496921 1109 54-55 0.4920286405975318 1109 56-57 0.48214932207123695 1109 58-59 0.48210129136243296 1109 60-61 0.5067738939565061 1109 62-63 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-5.740254050894034 2316 4 -6.88850483288039 2316 5 -5.090713307083835 2316 6 -3.9060478724172967 2316 7 -3.539638131025324 2316 8 -3.2863850571019952 2316 9 -3.4946954591099555 2316 10-11 -3.49676351494454 2316 12-13 -3.722356654293691 2316 14-15 -3.8948373044500784 2316 16-17 -3.970532167504693 2316 18-19 -4.0395302363372885 2316 20-21 -4.066708925578915 2316 22-23 -4.12301947973139 2316 24-25 -4.139517628920871 2316 26-27 -4.212625692792397 2316 28-29 -4.1708575017244485 2316 30-31 -4.3171598062729615 2316 32-33 -4.3143948481150005 2316 34-35 -4.294363012888034 2316 36-37 -4.358670859471655 2316 38-39 -4.3865959439237265 2316 40-41 -4.355899173774496 2316 42-43 -4.25978019324528 2316 44-45 -4.257644797437678 2316 46-47 -4.394083819867589 2316 48-49 -4.344588266324351 2316 50-51 -4.3671187635542665 2316 52-53 -4.407484388261679 2316 54-55 -4.396653720434294 2316 56-57 -4.437249312891581 2316 58-59 -4.42706993940282 2316 60-61 -4.468811501122065 2316 62-63 -4.456765398435387 2316 64-65 -4.344044235569466 2316 66-67 -4.484075184338124 2316 68-69 -4.377326391714401 2316 70-71 -4.344825102792328 2316 72-73 -4.31512336995673 2316 74-75 -4.247813101822409 2316 76-77 -4.119866142498413 2316 78-79 -4.2599256429063175 2316 80-81 -4.2664219054571895 2316 82-83 -4.401235679994915 2316 84-85 -4.302304180839492 2316 86-87 -4.277939107496593 2316 88-89 -4.199206836384015 2316 90-91 -4.219601519219726 2316 92-93 -4.219124682752469 2316 94-95 -4.2550409642270814 2316 96-97 -4.183402776341833 2316 98-99 -4.197148621392678 2316 100-101 -4.112952456454501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1488706.0 3 375971.0 4 239677.0 5 250129.0 6 436724.0 7 411307.0 8 357828.0 9 489789.0 10 648354.0 11 592293.0 12 434480.0 13 316903.0 14 263095.0 15 241480.0 16 230795.0 17 222179.0 18 215898.0 19 211281.0 20 211341.0 21 220393.0 22 244281.0 23 280838.0 24 325483.0 25 378693.0 26 444820.0 27 524666.0 28 626545.0 29 743161.0 30 884568.0 31 1085804.0 32 1295034.0 33 1628558.0 34 2135451.0 35 2927528.0 36 4319413.0 37 7470572.0 38 1.0764636E7 39 3396991.0 40 7054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.560691118264028 16.878468742155487 12.588763780060514 44.97207635951997 2 14.62567914075453 19.40355171524429 53.2129354068254 12.757833737175774 3 21.105032451270905 22.138177842603483 36.03389535494365 20.72289435118196 4 21.965259504487044 29.46433812822182 31.8415364215121 16.72886594577903 5 20.424236841059933 32.84036732372273 32.45351405121843 14.281881783998912 6 15.775047031511102 35.571391357452555 33.8313153245685 14.822246286467847 7 12.978193657238899 19.350337607690754 51.68045107485894 15.99101766021141 8 13.421969616303572 21.386560791485007 45.36633844226604 19.82513114994538 9 14.995568563631148 21.60494579354994 44.63890341211927 18.760582230699644 10-11 18.054022901183085 33.098317044479764 33.261942862109045 15.585717192228104 12-13 16.98273784381425 26.353013153118926 39.30288056068667 17.361368442380158 14-15 18.04874530127853 27.43283137710667 37.73615882216818 16.782264499446622 16-17 18.688382187661766 27.267670242692205 36.33815546552726 17.70579210411877 18-19 19.076134006733632 28.089040004002797 35.20823541350243 17.62659057576115 20-21 19.236017638455632 27.76980384672761 34.72556536510196 18.268613149714806 22-23 18.89437431669345 29.243713082591405 34.80012338378105 17.06178921693409 24-25 18.75213736517924 29.146456710572906 34.10391040897239 17.997495515275464 26-27 18.88351259389177 29.609298876109346 33.37481114576659 18.132377384232292 28-29 18.757973185180557 29.569942139213246 33.07574235881716 18.596342316789034 30-31 18.235786106240866 30.022961314328107 33.028300965382584 18.712951614048443 32-33 18.592235195432266 30.151041011086054 32.52008189741173 18.736641896069948 34-35 18.921941605810527 30.722640102682146 31.38059084773098 18.97482744377635 36-37 19.335137790495317 30.27521996093811 31.75778966532822 18.63185258323835 38-39 19.047510747844267 30.369909310438857 31.676280811607825 18.906299130109048 40-41 19.2330441864729 30.217487792736865 31.767987009799782 18.78148101099046 42-43 19.172806687517795 30.444910077267384 31.87258048928617 18.509702745928646 44-45 19.48434758948961 30.050430310274407 31.79689397396223 18.668328126273753 46-47 19.350234394484797 29.83740166165809 31.769546035618816 19.0428179082383 48-49 19.26653284168903 29.843970365420947 31.69212379350304 19.19737299938698 50-51 18.740868587122474 30.234251012506608 32.291517174738885 18.73336322563203 52-53 19.464857436257986 30.140120605150994 31.5898040644253 18.80521789416571 54-55 19.269737354637474 30.28231372861587 31.42816464079179 19.019784275954873 56-57 19.723138193267378 30.23514847580882 30.880977842455344 19.16073548846846 58-59 19.053941790631583 30.84642789488307 30.822269477066648 19.277360837418698 60-61 19.38667460819059 30.546956155372406 30.538257408498044 19.528111827938957 62-63 19.304011415067876 31.073856744879237 30.651837412257095 18.970294427795796 64-65 19.709291679835975 30.632217915565807 30.446159331773547 19.212331072824668 66-67 19.47668481325642 30.946512017880284 30.30990733317413 19.266895835689166 68-69 19.644714137221353 30.923512777057642 30.55418719433704 18.877585891383962 70-71 19.742277785261024 30.903590007846983 30.1895617874358 19.164570419456194 72-73 19.246641656148217 31.086548638227228 30.319660914233342 19.347148791391216 74-75 19.080884711877882 30.394675609581462 31.520841158732377 19.003598519808275 76-77 19.06469079694697 30.867065044344464 30.986114755012668 19.082129403695898 78-79 19.45287929940417 30.92660440476308 30.481104911417745 19.139411384415002 80-81 19.15858413629921 30.8265605851711 30.837606045957333 19.17724923257235 82-83 19.621036116606184 31.29510914757142 30.147905159669282 18.935949576153117 84-85 19.35191845969891 30.71677861838002 30.617486063965206 19.31381685795587 86-87 19.34764746696465 31.58755653069231 30.215327192425196 18.849468809917845 88-89 19.297542335771436 31.227114332333763 30.555732040806298 18.919611291088504 90-91 19.20573953285706 31.56222884603951 30.353220765759094 18.878810855344337 92-93 19.58123743089747 31.217083273725077 30.06446079874623 19.137218496631228 94-95 19.25485270918286 31.220651248399868 30.215040685290173 19.309455357127106 96-97 19.52898124035491 31.229838558886463 30.171025516306777 19.070154684451857 98-99 19.406524531893652 31.145388607587783 30.01692947502782 19.431157385490742 100-101 19.838826247880313 31.823714102727074 29.204879078492286 19.132580570900327 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9016.0 1 10530.5 2 11699.5 3 13459.0 4 18354.5 5 24734.0 6 31285.0 7 38183.5 8 46383.5 9 55303.0 10 64828.0 11 74930.5 12 85576.0 13 96593.5 14 107473.0 15 118784.0 16 130431.0 17 143894.5 18 161191.0 19 181475.5 20 210079.0 21 252042.0 22 311137.5 23 381842.0 24 458675.0 25 564644.0 26 735394.0 27 946859.5 28 1108046.5 29 1213565.5 30 1358933.5 31 1553811.0 32 1713826.5 33 1822014.0 34 1953318.0 35 2190778.5 36 2385420.0 37 2483390.5 38 2379771.0 39 2036885.0 40 1824936.0 41 1731707.5 42 1592642.0 43 1469247.0 44 1400162.5 45 1342427.0 46 1250908.0 47 1156567.0 48 1078515.0 49 1008666.0 50 922241.0 51 819706.5 52 735655.5 53 653157.0 54 564260.5 55 482789.0 56 411052.5 57 338176.5 58 268829.5 59 210364.0 60 156759.5 61 114128.0 62 82830.0 63 57532.0 64 39084.5 65 27884.0 66 20250.5 67 15074.0 68 11417.0 69 8496.5 70 7462.0 71 8148.0 72 8056.0 73 9470.0 74 9399.0 75 5919.5 76 4182.0 77 3729.5 78 3199.5 79 2058.5 80 1391.0 81 1074.0 82 776.0 83 565.0 84 523.5 85 416.0 86 275.5 87 196.0 88 130.0 89 88.0 90 57.0 91 34.0 92 22.0 93 12.5 94 6.5 95 4.0 96 2.5 97 0.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04357586643893436 2 0.003483112155007405 3 0.029056210311874987 4 0.006780345674696039 5 0.030498881992814985 6 0.11899823497674479 7 0.14118538481070342 8 0.13923365913985633 9 0.15550648875912682 10-11 0.15737583639841218 12-13 0.1634665300909312 14-15 0.1740278161885886 16-17 0.18602543719552736 18-19 0.1810954288451409 20-21 0.18375476068453103 22-23 0.1561919162268648 24-25 0.1679328979816305 26-27 0.17683289377612638 28-29 0.1822360477436879 30-31 0.1932799846160082 32-33 0.19462654859345951 34-35 0.19607978156049718 36-37 0.18796554545166702 38-39 0.20555325519009587 40-41 0.22559646394623004 42-43 0.2146760941212523 44-45 0.24326655171622058 46-47 0.23314883963466482 48-49 0.23104714370123103 50-51 0.23196280720589793 52-53 0.23321009509403123 54-55 0.2366879266059898 56-57 0.2340718960396001 58-59 0.24264449196506857 60-61 0.2482451419826563 62-63 0.25172086123739534 64-65 0.38961429317145896 66-67 0.20570533770990213 68-69 0.34731317396451183 70-71 0.01549340670526338 72-73 0.0027543834142690453 74-75 0.021549248153660123 76-77 0.03525462912259011 78-79 0.017340575643743656 80-81 0.023358396462188832 82-83 0.041806851017576745 84-85 0.02402798200078031 86-87 0.01603625681068297 88-89 0.030086991835006353 90-91 0.008350808917417692 92-93 0.02718052590093104 94-95 0.028541875678919075 96-97 0.024614133379200296 98-99 0.020259715121136158 100-101 0.011187570363248465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.7342719E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.758342613170488 #Duplication Level Percentage of deduplicated Percentage of total 1 75.8480547463433 23.32960454430539 2 8.91338458615639 5.483218738879021 3 4.07351075719262 3.7588331852449848 4 2.288338150813046 2.815419554299867 5 1.5353296387211801 2.3612097525970657 6 1.1248088568621446 2.0758353716216686 7 0.81116907827373 1.7465151498727165 8 0.6607208198575808 1.6258141879067494 9 0.4978823489538852 1.3782632283156335 >10 3.661436710883402 21.9312335423938 >50 0.35748566774160356 7.586569443383598 >100 0.20369788828445193 11.520986115577173 >500 0.013844875188101407 2.910722317227992 >1k 0.008839736519029185 5.4134958213546405 >5k 9.689277128701565E-4 2.030234768205451 >10k+ 5.272104966220684E-4 4.032044278814141 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 139235 0.2941001339614651 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 135269 0.28572292182880327 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 99381 0.2099182347342577 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 79552 0.16803428632816803 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 58536 0.1236430886024945 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 51007 0.107739903996642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.013112892818851406 0.0 0.0 0.0 0.0 2 0.01326286308143814 0.0 0.0 2.1122572195314763E-6 0.0 3 0.013480425575049882 6.3367716585944294E-6 0.0 4.224514439062953E-6 4.224514439062953E-6 4 0.015332875156578987 6.3367716585944294E-6 0.0 6.3367716585944294E-6 4.224514439062953E-6 5 0.015685622112240744 6.3367716585944294E-6 0.0 8.449028878125905E-6 4.224514439062953E-6 6 0.016103849041707977 6.3367716585944294E-6 0.0 1.0561286097657381E-5 4.224514439062953E-6 7 0.016346758621954095 6.3367716585944294E-6 0.0 1.689805775625181E-5 4.224514439062953E-6 8 0.016914955814008065 6.3367716585944294E-6 0.0 1.901031497578329E-5 4.224514439062953E-6 9 0.017265590512450288 6.3367716585944294E-6 0.0 2.323482941484624E-5 4.224514439062953E-6 10-11 0.01857624611716957 6.3367716585944294E-6 0.0 2.429095802461198E-5 4.224514439062953E-6 12-13 0.020445593756454924 6.3367716585944294E-6 1.0561286097657382E-6 2.5347086634377718E-5 4.224514439062953E-6 14-15 0.02282082699981807 6.3367716585944294E-6 2.1122572195314763E-6 2.7459343853909192E-5 4.224514439062953E-6 16-17 0.025372433721012097 6.3367716585944294E-6 2.1122572195314763E-6 2.851547246367493E-5 4.224514439062953E-6 18-19 0.028196521623525678 6.3367716585944294E-6 2.1122572195314763E-6 3.273998690273788E-5 5.280643048828691E-6 20-21 0.030967803095550975 6.3367716585944294E-6 2.1122572195314763E-6 4.1189015780863786E-5 6.3367716585944294E-6 22-23 0.03369367103735635 7.392900268360167E-6 2.1122572195314763E-6 4.3301273000395264E-5 6.3367716585944294E-6 24-25 0.03591048498925463 8.449028878125905E-6 2.1122572195314763E-6 4.752578743945822E-5 8.449028878125905E-6 26-27 0.03826248340320293 9.505157487891642E-6 2.1122572195314763E-6 6.231158797617855E-5 8.449028878125905E-6 28-29 0.040090642026707424 1.0561286097657381E-5 2.1122572195314763E-6 7.287287407383594E-5 8.449028878125905E-6 30-31 0.041939923222407235 1.0561286097657381E-5 2.1122572195314763E-6 7.392900268360168E-5 9.505157487891642E-6 32-33 0.04373322960178945 1.0561286097657381E-5 2.1122572195314763E-6 7.392900268360168E-5 1.0561286097657381E-5 34-35 0.045708190102051384 1.3729671926954598E-5 2.1122572195314763E-6 7.392900268360168E-5 1.0561286097657381E-5 36-37 0.04746664423731134 1.5841929146486072E-5 2.1122572195314763E-6 8.237803156172759E-5 1.0561286097657381E-5 38-39 0.049157506141546285 1.901031497578329E-5 2.1122572195314763E-6 8.237803156172759E-5 1.2673543317188859E-5 40-41 0.05085048030300077 1.901031497578329E-5 2.1122572195314763E-6 8.55464173910248E-5 1.3729671926954598E-5 42-43 0.05270081762731034 2.323482941484624E-5 2.1122572195314763E-6 9.39954462691507E-5 1.4785800536720335E-5 44-45 0.05476449293079259 2.323482941484624E-5 2.1122572195314763E-6 9.716383209844792E-5 1.4785800536720335E-5 46-47 0.05689259207947055 2.5347086634377718E-5 2.1122572195314763E-6 1.1406188985469972E-4 1.4785800536720335E-5 48-49 0.05920868212068682 2.5347086634377718E-5 2.1122572195314763E-6 1.246231759523571E-4 1.4785800536720335E-5 50-51 0.06171593144027068 2.5347086634377718E-5 2.1122572195314763E-6 1.2884769039142005E-4 1.4785800536720335E-5 52-53 0.06466675477595615 2.5347086634377718E-5 2.1122572195314763E-6 1.3095994761095153E-4 1.4785800536720335E-5 54-55 0.06760068005388536 2.5347086634377718E-5 2.1122572195314763E-6 1.3518446205001449E-4 1.689805775625181E-5 56-57 0.07077857104067048 2.5347086634377718E-5 2.1122572195314763E-6 1.4257736231837464E-4 1.9010314975783285E-5 58-59 0.07434300509862984 2.5347086634377718E-5 2.1122572195314763E-6 1.468018767574376E-4 2.21787008050805E-5 60-61 0.07843867184730138 2.6403215244143457E-5 2.1122572195314763E-6 1.520825198062663E-4 2.323482941484624E-5 62-63 0.08257024696870495 2.7459343853909192E-5 2.1122572195314763E-6 1.658121917332209E-4 2.323482941484624E-5 64-65 0.08711159999069762 2.7459343853909192E-5 2.1122572195314763E-6 1.7848573505040974E-4 2.429095802461198E-5 66-67 0.09237640111037983 2.851547246367493E-5 2.1122572195314763E-6 1.8587863531876992E-4 2.5347086634377718E-5 68-69 0.09838366064272734 2.957160107344067E-5 2.1122572195314763E-6 1.8693476392853565E-4 2.5347086634377718E-5 70-71 0.1051851288896187 2.957160107344067E-5 2.1122572195314763E-6 1.9221540697736435E-4 2.5347086634377718E-5 72-73 0.11287480129732304 2.957160107344067E-5 2.1122572195314763E-6 1.9432766419689583E-4 2.5347086634377718E-5 74-75 0.12211592663277324 2.957160107344067E-5 2.1122572195314763E-6 2.0172056446525602E-4 2.5347086634377718E-5 76-77 0.1329898267989213 2.957160107344067E-5 2.1122572195314763E-6 2.429095802461198E-4 2.5347086634377718E-5 78-79 0.14532012831793628 2.957160107344067E-5 2.1122572195314763E-6 2.587515093926059E-4 2.5347086634377718E-5 80-81 0.159058249273769 2.957160107344067E-5 2.1122572195314763E-6 2.672005382707318E-4 2.5347086634377718E-5 82-83 0.17552646268584615 2.957160107344067E-5 2.1122572195314763E-6 2.756495671488577E-4 2.5347086634377718E-5 84-85 0.195413364407735 2.957160107344067E-5 2.1122572195314763E-6 2.7881795297815494E-4 2.5347086634377718E-5 86-87 0.21929137614592858 2.957160107344067E-5 2.1122572195314763E-6 2.819863388074521E-4 2.5347086634377718E-5 88-89 0.2478353640820672 2.957160107344067E-5 2.1122572195314763E-6 2.872669818562808E-4 2.5347086634377718E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 36460 0.0 70.20575 1 GCGTGTT 45885 0.0 55.980453 2 CGTGTTT 68670 0.0 38.267273 3 CCTTTAG 52445 0.0 33.208824 5 GTTTTTT 666790 0.0 33.002647 1 TAGGCAT 54820 0.0 31.481283 9 TCGTCTA 42530 0.0 31.094297 44-45 TTAGGCA 55895 0.0 30.94289 8 ACTCGTC 42960 0.0 30.83378 42-43 TTTAGGC 59460 0.0 29.524973 7 CTAACGA 43325 0.0 29.511244 94-95 CCCGAAA 49615 0.0 28.791574 1 TCGACTC 3945 0.0 26.114979 3 CTTTAGG 68575 0.0 25.632414 6 CGCGTTT 3615 0.0 24.960073 1 GCCTAAC 51750 0.0 24.882013 92-93 CCGAAAC 58840 0.0 24.62303 2 GTCTATG 55480 0.0 24.10877 46-47 CCAAACG 66835 0.0 24.073666 8 TCCTTTA 71810 0.0 23.71296 4 >>END_MODULE