FastQCFastQC Report
Fri 27 May 2016
SRR1233033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60010497
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1536220.25599188088710545No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1524930.2541105433604391No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC1451700.24190767825168985No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1312370.21869007350497363No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1279550.21322103031407988No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA1075540.17922531119847251No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT904180.15067030689647512No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT835150.13916731934414742No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT800070.13332167537289352No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT684480.11406004519509312No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC680000.11331350913491019No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT665050.11082227830907648No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC636770.1061097694291717No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA632360.10537489799492912No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC608510.10140059329953557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT441100.055.5534441
GCGTGTT600900.040.4690172
GTTTTTT6232400.035.382481
TCGTCTA524800.031.34147344-45
CGTGTTT788100.031.2726043
ACTCGTC545200.030.26468842-43
CTAACGA577050.027.45341994-95
TTAGGCA537200.027.3077288
CCTTTAG551050.026.8729325
TAGGCAT560150.026.0954489
GTCTATG671050.024.72756246-47
CAAAACG396250.024.317459
ATGTGGC686200.024.3016650-51
GCCTAAC670750.023.94724792-93
CCCGAAA680400.023.9352321
CTATGTG721650.023.11308948-49
CCCGTAT59950.022.7570171
GAGGTGA709150.022.22392882-83
TTTAGGC707200.022.1924367
CACCAAT552350.022.0655631
Produced by FastQC (version 0.11.5)