##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60010497 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.239482902466214 31.0 31.0 34.0 26.0 34.0 2 30.429062802129433 31.0 31.0 34.0 26.0 34.0 3 30.376767051271045 31.0 31.0 34.0 25.0 34.0 4 33.78016929271557 37.0 35.0 37.0 28.0 37.0 5 33.750171757451035 37.0 35.0 37.0 28.0 37.0 6 33.782568173031464 37.0 35.0 37.0 28.0 37.0 7 33.91419207876249 37.0 35.0 37.0 28.0 37.0 8 33.87847666050824 37.0 35.0 37.0 28.0 37.0 9 35.32565729292327 39.0 35.0 39.0 28.0 39.0 10-11 35.23489667149399 39.0 35.0 39.0 27.5 39.0 12-13 35.124540011724946 39.0 35.0 39.0 27.0 39.0 14-15 36.111144055347516 39.5 36.0 41.0 27.0 41.0 16-17 35.9228979306737 39.0 36.0 41.0 27.0 41.0 18-19 35.657199714576606 39.0 35.5 41.0 26.5 41.0 20-21 35.453142506051904 39.0 35.0 41.0 25.5 41.0 22-23 35.20887601547443 39.0 34.0 41.0 25.0 41.0 24-25 34.98168702052243 39.0 34.0 41.0 24.0 41.0 26-27 34.668729822384236 39.0 34.0 41.0 20.5 41.0 28-29 34.526466053097344 39.0 34.0 41.0 18.5 41.0 30-31 34.515469443620844 39.0 34.0 41.0 18.0 41.0 32-33 34.40087171749302 39.0 34.0 41.0 17.0 41.0 34-35 34.264639609633626 39.0 34.0 41.0 16.0 41.0 36-37 34.11600225540542 39.0 34.0 41.0 16.0 41.0 38-39 33.98975974153322 39.0 33.5 41.0 15.5 41.0 40-41 33.809169294165315 38.5 33.0 41.0 15.0 41.0 42-43 33.6667952525039 38.0 33.0 40.5 13.5 41.0 44-45 33.40724332777981 38.0 33.0 40.0 9.0 41.0 46-47 33.31993588555015 38.0 33.0 40.0 9.0 41.0 48-49 33.1966369067065 38.0 33.0 40.0 9.0 41.0 50-51 32.704387392425694 37.5 32.0 39.5 8.0 40.5 52-53 32.84595050095986 38.0 32.5 40.0 7.5 41.0 54-55 33.08508975521399 38.0 33.0 40.0 6.0 41.0 56-57 32.96831159388665 38.0 32.5 40.0 4.0 41.0 58-59 32.77931668354621 38.0 32.0 40.0 2.0 41.0 60-61 32.68400857436658 38.0 32.0 40.0 2.0 41.0 62-63 32.44976997940877 38.0 31.5 40.0 2.0 41.0 64-65 32.16476744893481 37.0 31.0 40.0 2.0 41.0 66-67 31.900309415867696 37.0 31.0 40.0 2.0 41.0 68-69 31.548696947135767 36.5 31.0 39.0 2.0 41.0 70-71 31.178219220547366 36.0 30.5 39.0 2.0 41.0 72-73 30.83973837110531 35.5 30.0 39.0 2.0 40.5 74-75 30.428624512141603 35.0 30.0 38.0 2.0 40.0 76-77 30.032389625101757 35.0 29.0 37.0 2.0 39.5 78-79 29.652608184531452 35.0 29.0 37.0 2.0 39.0 80-81 29.251050853653155 35.0 29.0 36.0 2.0 39.0 82-83 28.874277161877195 34.0 29.0 36.0 2.0 38.0 84-85 28.521141901224382 34.0 28.5 36.0 2.0 37.0 86-87 28.143341505736906 34.0 27.0 35.0 2.0 37.0 88-89 27.854509128627946 34.0 27.0 35.0 2.0 36.0 90-91 27.568551548573247 34.0 26.0 35.0 2.0 36.0 92-93 27.238326513109865 34.0 26.0 35.0 2.0 36.0 94-95 26.835396855653435 34.0 25.0 35.0 2.0 35.5 96-97 26.51680128561508 34.0 25.0 35.0 2.0 35.0 98-99 26.11649729379845 34.0 24.5 35.0 2.0 35.0 100-101 24.524842628782096 32.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1590311713264754 1101 2 1.279402061367108 1101 3 1.3496944564101554 1101 4 1.3790919573360654 1101 5 1.2999088833770287 1101 6 1.349447614309483 1101 7 1.305915018512799 1101 8 1.2393522580118344 1101 9 1.4691420156029338 1101 10-11 1.3777531580277085 1101 12-13 1.3595868028950946 1101 14-15 1.5234058795092693 1101 16-17 1.5318879120045779 1101 18-19 1.6023595704066764 1101 20-21 1.5578476670396242 1101 22-23 1.704987938717295 1101 24-25 1.665466205087398 1101 26-27 1.5556534220494882 1101 28-29 1.5726058161394079 1101 30-31 1.5299482480178455 1101 32-33 1.671717519251267 1101 34-35 1.597175749534479 1101 36-37 1.5629113548729379 1101 38-39 1.6112039221634618 1101 40-41 1.6247107869417121 1101 42-43 1.6241479276868986 1101 44-45 1.61885722745928 1101 46-47 1.6070869761255295 1101 48-49 1.499987601083859 1101 50-51 1.5514103036345617 1101 52-53 1.7056257473843672 1101 54-55 1.665890317718457 1101 56-57 1.6976626568344884 1101 58-59 1.7633312338674614 1101 60-61 1.7925837655994812 1101 62-63 1.7317321719552012 1101 64-65 1.6656269526821745 1101 66-67 1.683096132872901 1101 68-69 1.762265955624379 1101 70-71 1.6917734449776987 1101 72-73 1.751085308204658 1101 74-75 1.762670850878422 1101 76-77 1.671479682521369 1101 78-79 1.7233326746940563 1101 80-81 1.7576245130019217 1101 82-83 1.7280402642942718 1101 84-85 1.7749366834256506 1101 86-87 1.8458906186690491 1101 88-89 1.776434622844988 1101 90-91 1.7926279094495357 1101 92-93 1.7067267552198722 1101 94-95 1.8632216362259975 1101 96-97 1.9964452618325375 1101 98-99 2.0578960109437467 1101 100-101 2.1831459895471816 1102 1 1.0364406758974063 1102 2 1.1932252014321563 1102 3 1.246685958996121 1102 4 1.2614529204079403 1102 5 1.2838030892808732 1102 6 1.2225144198700733 1102 7 1.2178681971956067 1102 8 1.212210886084769 1102 9 1.3009748517451385 1102 10-11 1.3392194212626052 1102 12-13 1.3772149619658194 1102 14-15 1.5658082196117462 1102 16-17 1.5502463661038277 1102 18-19 1.565793078379265 1102 20-21 1.5892046698715205 1102 22-23 1.5345940449291788 1102 24-25 1.550761247384557 1102 26-27 1.5122286030823489 1102 28-29 1.5236910108561545 1102 30-31 1.5629114643792903 1102 32-33 1.5489461106578304 1102 34-35 1.5893182197174482 1102 36-37 1.5816254704793522 1102 38-39 1.5418681218614338 1102 40-41 1.5787482381540627 1102 42-43 1.5344774819344806 1102 44-45 1.674503365915264 1102 46-47 1.6301895588978965 1102 48-49 1.6155190443193561 1102 50-51 1.566874920500716 1102 52-53 1.6944629113322662 1102 54-55 1.7223073504469042 1102 56-57 1.6863428095807862 1102 58-59 1.800179984034287 1102 60-61 1.7578323936088651 1102 62-63 1.7266234396820295 1102 64-65 1.7710983106238558 1102 66-67 1.7471146473768293 1102 68-69 1.7717809279926655 1102 70-71 1.7973380811625148 1102 72-73 1.7808488375187395 1102 74-75 1.725492906724476 1102 76-77 1.6765736013413708 1102 78-79 1.6677142948872792 1102 80-81 1.702530436488857 1102 82-83 1.6736434235240445 1102 84-85 1.7579191885184677 1102 86-87 1.7401040692507657 1102 88-89 1.7306778670120089 1102 90-91 1.7233696823777578 1102 92-93 1.7076823476416543 1102 94-95 1.7290157034466418 1102 96-97 1.8741875669380335 1102 98-99 1.9485006207688222 1102 100-101 2.0605555512532767 1103 1 0.9802672774097267 1103 2 1.1052073304296144 1103 3 1.1439741604154428 1103 4 1.1473453181615128 1103 5 1.1636792169290686 1103 6 1.2296089365912835 1103 7 1.1957420549786377 1103 8 1.180316831840706 1103 9 1.26564168664585 1103 10-11 1.2821652580600826 1103 12-13 1.287146503717409 1103 14-15 1.5611797839096653 1103 16-17 1.4988103928857228 1103 18-19 1.530395930836555 1103 20-21 1.5474400213758486 1103 22-23 1.474630005795575 1103 24-25 1.4863694913799037 1103 26-27 1.4665737274821282 1103 28-29 1.5261838990367877 1103 30-31 1.5373490916960932 1103 32-33 1.5586708262036097 1103 34-35 1.5446663346017218 1103 36-37 1.5865580075195425 1103 38-39 1.58217601805665 1103 40-41 1.5726485749784374 1103 42-43 1.535487048429239 1103 44-45 1.6318385698923095 1103 46-47 1.6749644837848336 1103 48-49 1.6406894526006965 1103 50-51 1.5904302540375141 1103 52-53 1.6619247140396212 1103 54-55 1.7442440696738188 1103 56-57 1.7235278368695575 1103 58-59 1.8926355772105339 1103 60-61 1.7858345102866622 1103 62-63 1.7309128458219618 1103 64-65 1.7255507328364388 1103 66-67 1.7044886046714929 1103 68-69 1.6212802704188363 1103 70-71 1.812284231816541 1103 72-73 1.7626322301445185 1103 74-75 1.689531467422782 1103 76-77 1.7320638495642733 1103 78-79 1.687792866734771 1103 80-81 1.7336951386792414 1103 82-83 1.7331456046397804 1103 84-85 1.7385128483120198 1103 86-87 1.6889969607902025 1103 88-89 1.705319133916607 1103 90-91 1.7290724189910556 1103 92-93 1.7132344539657218 1103 94-95 1.7955318527882334 1103 96-97 1.853727351918458 1103 98-99 1.9212536563269502 1103 100-101 1.996903615480658 1104 1 0.8664637027485291 1104 2 0.9702769622082386 1104 3 1.0460314483822728 1104 4 1.0373597745026828 1104 5 0.9940965261815151 1104 6 0.9889810801402348 1104 7 1.004721240783148 1104 8 1.0293455269570444 1104 9 1.0595841421298289 1104 10-11 1.0354054157272614 1104 12-13 1.1191449842076793 1104 14-15 1.305006006740669 1104 16-17 1.2477416246584667 1104 18-19 1.3209014695771373 1104 20-21 1.3181801610868362 1104 22-23 1.3504839614264483 1104 24-25 1.3881261091588541 1104 26-27 1.358047201769942 1104 28-29 1.349739000899099 1104 30-31 1.3414051483073663 1104 32-33 1.3760824375848273 1104 34-35 1.3860722282640623 1104 36-37 1.4212353006129916 1104 38-39 1.4047789753689912 1104 40-41 1.4089775591971332 1104 42-43 1.4232839388559881 1104 44-45 1.5424720922338224 1104 46-47 1.5003395761340457 1104 48-49 1.413180648196203 1104 50-51 1.302529331144278 1104 52-53 1.4378221637970796 1104 54-55 1.5389721520697037 1104 56-57 1.5632188462962588 1104 58-59 1.6011214953973507 1104 60-61 1.5135360296752935 1104 62-63 1.4367700086692707 1104 64-65 1.371722701972871 1104 66-67 1.5141998226867592 1104 68-69 1.4976735524212899 1104 70-71 1.4866685718678667 1104 72-73 1.4878211739246225 1104 74-75 1.550335071847151 1104 76-77 1.5521873205898054 1104 78-79 1.4733763046746269 1104 80-81 1.5067425124353946 1104 82-83 1.4168917405377677 1104 84-85 1.496432212678446 1104 86-87 1.4692633809540538 1104 88-89 1.4938001014202236 1104 90-91 1.5001618514635098 1104 92-93 1.5416855208033375 1104 94-95 1.5479342686634467 1104 96-97 1.6052920511800508 1104 98-99 1.7304694682524406 1104 100-101 1.809159581673491 1105 1 0.8383887464377118 1105 2 0.9238519939160419 1105 3 0.9797972954545493 1105 4 1.0423377219617151 1105 5 1.0346982989274522 1105 6 1.0638166021754003 1105 7 1.0177274861847678 1105 8 0.9815216840077028 1105 9 1.087306186548794 1105 10-11 1.0653641621821137 1105 12-13 1.0189942825131624 1105 14-15 1.1366017768129524 1105 16-17 1.1008735746493343 1105 18-19 1.139045741467946 1105 20-21 1.1313457775805063 1105 22-23 1.1533331907996214 1105 24-25 1.1884398060196943 1105 26-27 1.1736556006112338 1105 28-29 1.1877320332204562 1105 30-31 1.2326904884003866 1105 32-33 1.1906057031919843 1105 34-35 1.1485647191818416 1105 36-37 1.2116483225189967 1105 38-39 1.1961663389839643 1105 40-41 1.251734652383881 1105 42-43 1.2155509969622855 1105 44-45 1.2985596576304346 1105 46-47 1.265433781506644 1105 48-49 1.288975560382113 1105 50-51 1.2071482400443259 1105 52-53 1.210063991304942 1105 54-55 1.3886194260116014 1105 56-57 1.389549964243649 1105 58-59 1.4925593392666414 1105 60-61 1.4127278652196793 1105 62-63 1.3889162023971693 1105 64-65 1.384928013884121 1105 66-67 1.448491663829227 1105 68-69 1.404430471569043 1105 70-71 1.447988746814417 1105 72-73 1.354150003778095 1105 74-75 1.440169374253479 1105 76-77 1.4171695336288188 1105 78-79 1.4241094262676732 1105 80-81 1.4156950250115905 1105 82-83 1.3929102325387497 1105 84-85 1.3838835053113456 1105 86-87 1.3507097157193364 1105 88-89 1.3566759220236086 1105 90-91 1.2957114416326014 1105 92-93 1.424157197265938 1105 94-95 1.3548429961330832 1105 96-97 1.4402121066519342 1105 98-99 1.4293989294470286 1105 100-101 1.5251106508491965 1106 1 0.6540597487267128 1106 2 0.745580438966627 1106 3 0.76976049164195 1106 4 0.8275604888091692 1106 5 0.8093592882862879 1106 6 0.7776464187322603 1106 7 0.8252456509837387 1106 8 0.8412884408470731 1106 9 0.9186864189042865 1106 10-11 0.81695040359903 1106 12-13 0.8430651366111874 1106 14-15 0.9652981171351058 1106 16-17 0.9588465716251875 1106 18-19 1.0355732925468573 1106 20-21 0.945172736520739 1106 22-23 1.0184665868977802 1106 24-25 1.023374600844143 1106 26-27 1.0139355475308918 1106 28-29 0.9494540514956427 1106 30-31 0.9392404055030781 1106 32-33 0.9632771265061066 1106 34-35 0.9756730128130826 1106 36-37 1.0280077066017839 1106 38-39 0.9969094646106882 1106 40-41 1.0425893236888442 1106 42-43 0.9800602702144587 1106 44-45 1.1087333584518007 1106 46-47 1.0490598715729078 1106 48-49 0.9608908116626083 1106 50-51 0.9413687143699434 1106 52-53 1.0101653420285572 1106 54-55 1.0842705297781379 1106 56-57 1.0261544305619523 1106 58-59 1.1403588599796066 1106 60-61 1.1384925242426078 1106 62-63 1.0926346793412165 1106 64-65 1.1663061195388025 1106 66-67 1.1137355250830971 1106 68-69 1.1548659608955987 1106 70-71 1.1644147675229348 1106 72-73 1.1828085214086244 1106 74-75 1.1477926588359004 1106 76-77 1.1555194401112239 1106 78-79 1.1657975705578494 1106 80-81 1.16776972513577 1106 82-83 1.1007921996744834 1106 84-85 1.1944247334982379 1106 86-87 1.1938976067868161 1106 88-89 1.151981144100052 1106 90-91 1.1669995011013228 1106 92-93 1.1981168817110905 1106 94-95 1.155388730459979 1106 96-97 1.1029342202984225 1106 98-99 1.187063924664816 1106 100-101 1.3106266866577307 1107 1 0.5598351916046056 1107 2 0.6733654453653664 1107 3 0.6499591583882705 1107 4 0.7379842989197982 1107 5 0.7652746130364747 1107 6 0.733413715883465 1107 7 0.7108645809503074 1107 8 0.7078622192935029 1107 9 0.7858536665702616 1107 10-11 0.8154193821609752 1107 12-13 0.8402653106175677 1107 14-15 0.9377681462961789 1107 16-17 0.8936829707050933 1107 18-19 0.8581937025970134 1107 20-21 0.8921520523874449 1107 22-23 0.8063201299545995 1107 24-25 0.876667519877202 1107 26-27 0.8903576586550557 1107 28-29 0.8338307220670629 1107 30-31 0.8031041425267986 1107 32-33 0.7439445128442372 1107 34-35 0.7858329021004664 1107 36-37 0.8519197686841977 1107 38-39 0.7770497416240758 1107 40-41 0.8711704720578624 1107 42-43 0.9205753495009716 1107 44-45 0.9711050049352394 1107 46-47 0.8026599244251358 1107 48-49 0.7306571077095327 1107 50-51 0.7800670080049414 1107 52-53 0.7914536446207237 1107 54-55 0.9376418156562494 1107 56-57 0.9095809286902394 1107 58-59 0.9914391331700116 1107 60-61 0.9279924564697808 1107 62-63 0.835805953357891 1107 64-65 0.8589139856027543 1107 66-67 0.8887750909002285 1107 68-69 0.9344521324867827 1107 70-71 0.9378695782443387 1107 72-73 0.892808133242017 1107 74-75 1.0078654643646274 1107 76-77 0.9593828774722439 1107 78-79 0.992914213708449 1107 80-81 0.9434413483580784 1107 82-83 0.9148173408935314 1107 84-85 0.9599999545583984 1107 86-87 0.9037948198371772 1107 88-89 1.0032960909385444 1107 90-91 0.9928566049494023 1107 92-93 1.057071621917963 1107 94-95 0.9781925456230276 1107 96-97 1.0635721365181183 1107 98-99 1.1684053524502893 1107 100-101 1.213302189910685 1108 1 0.40798933564018824 1108 2 0.49472406150828974 1108 3 0.5239081542007575 1108 4 0.5295449292807817 1108 5 0.59215187172871 1108 6 0.4907363529350093 1108 7 0.5544909281765626 1108 8 0.4916377177901907 1108 9 0.4825439730756358 1108 10-11 0.5811010916533732 1108 12-13 0.47045769403656124 1108 14-15 0.620426373476576 1108 16-17 0.5399788477437468 1108 18-19 0.6121045648414878 1108 20-21 0.5872789775943446 1108 22-23 0.5635483509005894 1108 24-25 0.5970318829628667 1108 26-27 0.5529180541226708 1108 28-29 0.5909926571586297 1108 30-31 0.5542409187322406 1108 32-33 0.5789784220071041 1108 34-35 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-0.2578302072143259 2311 50-51 -0.27791517252822473 2311 52-53 -0.231350129508769 2311 54-55 -0.23958573229231916 2311 56-57 -0.14955600996495377 2311 58-59 -0.30659069888633184 2311 60-61 -0.3817915968919863 2311 62-63 -0.3144789656789584 2311 64-65 -0.26738235382039477 2311 66-67 -0.32288597071685743 2311 68-69 -0.3193576336020314 2311 70-71 -0.23441389690694336 2311 72-73 -0.21138108033174774 2311 74-75 -0.28609639791703145 2311 76-77 -0.34065890391372733 2311 78-79 -0.27125388152578367 2311 80-81 -0.1934250646811151 2311 82-83 -0.21269642448951132 2311 84-85 -0.23510274393495934 2311 86-87 -0.19439049875091285 2311 88-89 -0.15277863747855136 2311 90-91 -0.13678260911689222 2311 92-93 -0.18091732418452366 2311 94-95 -0.15808615178248075 2311 96-97 -0.221763737600849 2311 98-99 -0.16367697977679896 2311 100-101 -0.03110018126259817 2312 1 0.027721839451729124 2312 2 0.09662207359653152 2312 3 0.0035473888641348594 2312 4 -0.043692152399806616 2312 5 -0.0846820158182382 2312 6 -0.06841857002282836 2312 7 0.015796504583853732 2312 8 -0.007513156110078967 2312 9 -0.14205181105790388 2312 10-11 -0.14495943635785835 2312 12-13 -0.1936145869463246 2312 14-15 -0.11849149015647953 2312 16-17 -0.1450073660083575 2312 18-19 -0.19582995793619773 2312 20-21 -0.287476810376873 2312 22-23 -0.339580902183215 2312 24-25 -0.12263964864131083 2312 26-27 -0.1347986020865548 2312 28-29 -0.1498031990974411 2312 30-31 -0.3094961987796765 2312 32-33 -0.19292996981804578 2312 34-35 -0.11032698089933746 2312 36-37 -0.10195861920709604 2312 38-39 -0.09758551119416126 2312 40-41 -0.011779180941530853 2312 42-43 -0.021574738428576268 2312 44-45 0.03430685053488958 2312 46-47 0.007506301118937131 2312 48-49 -0.02899366508171397 2312 50-51 -0.18488594246475998 2312 52-53 -0.26717133717862396 2312 54-55 -0.13933564765510198 2312 56-57 -0.06064675643915507 2312 58-59 -0.1766055918924465 2312 60-61 -0.1200157806550024 2312 62-63 -0.09503704594055051 2312 64-65 -0.10983036717172467 2312 66-67 -0.11531755749105344 2312 68-69 -0.1277008527285588 2312 70-71 -0.13048438267295026 2312 72-73 -0.15531221073418067 2312 74-75 -0.08315612099644909 2312 76-77 -0.14180603600527064 2312 78-79 -0.1182154221909606 2312 80-81 -0.05593523446629689 2312 82-83 -0.2798327586743383 2312 84-85 -0.14178658386579457 2312 86-87 0.005303309752648033 2312 88-89 -0.33278224996169925 2312 90-91 -0.31452349325937234 2312 92-93 -0.12420414352506626 2312 94-95 -0.05422749269341054 2312 96-97 -0.052048602407793965 2312 98-99 -0.0029311371670956987 2312 100-101 0.033990572695525856 2313 1 -0.5659405911049262 2313 2 -0.4426504571520766 2313 3 -0.9723384630605061 2313 4 -1.04310455526047 2313 5 -1.1402120585200777 2313 6 -0.7839206646072086 2313 7 -0.7088218210872625 2313 8 -0.6608062878162997 2313 9 -0.9009980275777565 2313 10-11 -1.0005683867034776 2313 12-13 -0.9343902809326181 2313 14-15 -0.9860133031276774 2313 16-17 -1.173423549142349 2313 18-19 -0.9646682331710963 2313 20-21 -1.0544365452260536 2313 22-23 -1.0556230908642732 2313 24-25 -1.012106463573815 2313 26-27 -1.0128563648548337 2313 28-29 -1.0452884564404954 2313 30-31 -1.0671889064240645 2313 32-33 -1.1106735743175804 2313 34-35 -1.1145519950124125 2313 36-37 -1.0244242359687235 2313 38-39 -1.07243583765203 2313 40-41 -1.026546081673395 2313 42-43 -0.9945404648774314 2313 44-45 -0.8863027175874123 2313 46-47 -0.9554630392536794 2313 48-49 -1.0473309384123155 2313 50-51 -1.0115968394129062 2313 52-53 -1.024201512247874 2313 54-55 -1.1848051446844288 2313 56-57 -1.0775304231137355 2313 58-59 -1.1691079852742625 2313 60-61 -1.0781582203281062 2313 62-63 -1.1376561709145179 2313 64-65 -1.0489925327437177 2313 66-67 -1.2178358448286026 2313 68-69 -1.1105154646644806 2313 70-71 -1.0639790738374089 2313 72-73 -1.145201001995332 2313 74-75 -1.1728579610224443 2313 76-77 -1.198878997054038 2313 78-79 -1.205116862998306 2313 80-81 -1.1749849585832095 2313 82-83 -1.0922626765479357 2313 84-85 -1.016468841103336 2313 86-87 -1.0304576618897805 2313 88-89 -1.0946267967923724 2313 90-91 -1.0451134612316153 2313 92-93 -0.9876847520149106 2313 94-95 -0.9733949911573525 2313 96-97 -1.0289258665038865 2313 98-99 -1.0061756028149027 2313 100-101 -1.0021121938828657 2315 1 -2.391067894207737 2315 2 -1.9854390072341097 2315 3 -2.0809088841971715 2315 4 -2.3744102561647793 2315 5 -2.8430326263450425 2315 6 -2.6938758701495686 2315 7 -2.4090096501794207 2315 8 -2.3908995064597676 2315 9 -2.8420073219984374 2315 10-11 -2.7384664752562387 2315 12-13 -2.7807808257546327 2315 14-15 -3.3190101069927778 2315 16-17 -3.1733026925624728 2315 18-19 -3.2652984015364694 2315 20-21 -3.1808641943977918 2315 22-23 -3.4254179011875507 2315 24-25 -3.3473600049043384 2315 26-27 -3.513100760747143 2315 28-29 -3.718507514841029 2315 30-31 -3.747381617895904 2315 32-33 -3.8312326000847783 2315 34-35 -3.461108658965596 2315 36-37 -3.309465039958475 2315 38-39 -3.384391219498447 2315 40-41 -3.3915661517433904 2315 42-43 -3.343605460308119 2315 44-45 -3.1989363417112884 2315 46-47 -3.2841019283399078 2315 48-49 -3.2851860481605826 2315 50-51 -3.2900561427056587 2315 52-53 -3.3104137391429447 2315 54-55 -3.3219406610041773 2315 56-57 -3.201996303644883 2315 58-59 -3.342958044848494 2315 60-61 -3.599529935553612 2315 62-63 -3.6139299257742294 2315 64-65 -3.3435606206802007 2315 66-67 -3.398223011980175 2315 68-69 -3.4358060549184586 2315 70-71 -3.44517221699099 2315 72-73 -3.4048833451287095 2315 74-75 -3.267573436006913 2315 76-77 -3.4798161559379714 2315 78-79 -3.499372325501785 2315 80-81 -3.3606684058679654 2315 82-83 -3.418825668087589 2315 84-85 -3.349136019095056 2315 86-87 -3.3001981447204223 2315 88-89 -3.403941614649092 2315 90-91 -3.2307211239274203 2315 92-93 -3.168686474088023 2315 94-95 -3.1834662201925212 2315 96-97 -3.2727661086317816 2315 98-99 -3.341566150343464 2315 100-101 -3.2842950536432376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 929909.0 3 245358.0 4 232474.0 5 241240.0 6 293054.0 7 392029.0 8 506740.0 9 624474.0 10 650163.0 11 540896.0 12 434539.0 13 374816.0 14 344303.0 15 327211.0 16 323338.0 17 328957.0 18 339897.0 19 361187.0 20 393890.0 21 435044.0 22 484595.0 23 543803.0 24 609497.0 25 683192.0 26 772051.0 27 877283.0 28 1006581.0 29 1170329.0 30 1369815.0 31 1629640.0 32 1974345.0 33 2452830.0 34 3151916.0 35 4251927.0 36 6007681.0 37 9474708.0 38 1.1723957E7 39 3498844.0 40 7984.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.7935348857889 15.361130968151194 14.278081796454895 43.56725234960501 2 16.427349329259155 19.525634289262435 49.33383638882671 14.713179992651696 3 20.4748802014636 23.83115147740886 33.3249677674423 22.369000553685236 4 22.288999510391236 30.951911395852864 28.469504726479144 18.289584367276756 5 21.119597035118513 33.50147551707453 30.142128779772403 15.23679866803455 6 16.590337254977914 36.576545951296495 30.53593824234176 16.297178551383833 7 13.569507356675963 19.558063873873134 49.25741054553266 17.61501822391824 8 15.036020726428562 21.955717184229346 40.930229089928396 22.078032999413693 9 16.35826341470868 23.426102210730907 39.7248091407661 20.490825233794315 10-11 19.84034129524509 34.15262365249055 29.11422586092177 16.892809191342593 12-13 18.537705974730905 27.012726450789565 35.65326251617771 18.79630505830182 14-15 19.18564665274851 28.40173921387415 34.61366791171952 17.798946221657825 16-17 20.119767929051008 27.97285398143287 33.042359481654124 18.865018607861995 18-19 20.223090114150263 28.817742417089676 32.42694917035463 18.532218298405432 20-21 20.4352176876567 28.44027859157512 31.71660639414082 19.40789732662736 22-23 19.950291595077672 29.877531218216934 32.09000915150494 18.08216803520046 24-25 19.40395812074776 29.91414372460655 31.526665934197535 19.155232220448156 26-27 19.67320226604721 30.115654086338655 31.123136381108996 19.088007266505137 28-29 19.82580849210351 29.659057027029206 31.180573805671806 19.334560675195476 30-31 19.175260657188804 30.124327196918166 31.181054073397043 19.519358072495987 32-33 19.330847747861927 29.477145636928594 31.392684116374546 19.79932249883493 34-35 19.81822125788483 30.45712261827549 30.111350121547765 19.613306002291917 36-37 20.14735466172128 30.07962766352787 30.633372839574296 19.139644835176554 38-39 19.8333772052278 29.75281629749016 30.939429072240245 19.474377425041798 40-41 20.12303539475166 29.944011916749236 30.334686406897827 19.598266281601276 42-43 20.02030198030325 30.08663619799108 30.574071186152317 19.31899063555335 44-45 19.917391157332514 29.710678494242053 30.639379756322572 19.732550592102868 46-47 19.82232632545897 29.542264827394604 30.93349802634371 19.701910820802716 48-49 19.690051066532018 29.45776045839439 30.615921312838328 20.23626716223526 50-51 19.21435070502024 29.75448242761115 31.62143816865653 19.40972869871208 52-53 20.332647895426913 29.65198720927168 30.583384814111824 19.431980081189586 54-55 19.65158708794442 30.177867834705612 30.10344898479738 20.067096092552582 56-57 20.254308746761392 29.784695037347063 29.96630776383531 19.994688452056227 58-59 19.433342424291663 30.481502628893786 30.2859474684076 19.799207478406956 60-61 19.98023574582113 30.208017561970152 29.587923697314245 20.223822994894476 62-63 19.778258452644728 30.528834325076808 30.022269518586636 19.670637703691828 64-65 20.38752848989684 29.629936449939155 30.112931825829637 19.869603234334367 66-67 20.04976330644721 30.340493546172116 29.606028897670083 20.00371424971059 68-69 20.139518379802183 30.23125956335772 30.10864126151933 19.520580795320765 70-71 19.987364669957135 30.398604395536204 29.827340620327515 19.786690314179147 72-73 19.974230989327204 30.652235275430545 29.658894098242506 19.714639636999742 74-75 19.65613465417391 29.57392360925033 30.98689161576793 19.783050120807825 76-77 19.68042524007661 30.185741032943465 30.343943345582126 19.789890381397807 78-79 19.87324049155029 30.708844644185007 29.826871918602464 19.591042945662238 80-81 19.896312553144714 30.54836256730552 30.112997050082548 19.442327829467217 82-83 20.223784124555216 30.40378814091363 29.833687265396534 19.538740469134616 84-85 20.084875912060067 30.33962290775445 29.48996225194521 20.085538928240265 86-87 19.85557347666738 30.830487752641 29.66302021094619 19.650918559745435 88-89 19.770610135663706 30.32236736797752 30.224260482403164 19.682762013955607 90-91 19.758187655357016 30.853114851059406 29.814370153717046 19.57432733986654 92-93 20.203982511326444 30.053348958956903 29.78577639908904 19.956892130627605 94-95 19.977640862789638 30.51353346886479 29.286152330772563 20.222673337573006 96-97 20.015216192481432 30.43442993479959 29.349122796277943 20.201231076441033 98-99 20.303370743590783 30.191775762608884 29.523760237287817 19.98109325651252 100-101 20.967863098679153 31.19688310658765 28.18325587744 19.651997917293198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 37033.0 1 34112.0 2 26218.5 3 22860.5 4 25730.5 5 29650.0 6 32056.5 7 33258.5 8 36209.5 9 39345.5 10 43249.0 11 47876.0 12 53915.5 13 59808.0 14 66768.5 15 76298.5 16 87796.5 17 100176.5 18 115297.0 19 136444.0 20 170773.5 21 219098.5 22 286775.5 23 346054.5 24 415830.0 25 541542.5 26 742097.5 27 1044681.5 28 1240093.0 29 1311232.0 30 1499559.5 31 1746919.5 32 1925947.0 33 2133349.0 34 2441599.0 35 2785980.5 36 3000892.5 37 3183339.5 38 3298795.5 39 2807902.0 40 2401051.0 41 2289484.5 42 1954609.5 43 1745540.5 44 1656880.5 45 1563399.5 46 1470985.5 47 1433488.5 48 1414644.5 49 1348255.0 50 1292339.5 51 1257120.0 52 1135158.5 53 1006738.5 54 929837.5 55 838918.0 56 758637.5 57 661015.0 58 565011.0 59 477584.0 60 380845.0 61 286968.5 62 214837.5 63 156107.5 64 105295.0 65 73647.5 66 51347.0 67 38137.5 68 29058.5 69 21335.0 70 19021.5 71 22578.5 72 23102.5 73 26327.5 74 27179.5 75 19819.5 76 16198.0 77 15885.5 78 14178.0 79 10485.0 80 7203.5 81 4802.0 82 3534.0 83 2733.0 84 2185.0 85 1995.5 86 1911.0 87 1514.0 88 980.0 89 625.0 90 517.0 91 445.0 92 356.5 93 239.0 94 161.5 95 97.5 96 43.5 97 22.5 98 15.0 99 10.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6427008928121358 2 0.28468852707552145 3 0.30976747284729206 4 0.2779463732819943 5 0.31115056420879167 6 0.19610235855903677 7 0.09248048720543008 8 0.10671133085266733 9 0.10112564140237 10-11 0.0895318364052209 12-13 0.09285292204795438 14-15 0.09244799289031051 16-17 0.08324293664823339 18-19 0.10530990936468998 20-21 0.09725131921503666 22-23 0.09038085453616557 24-25 0.1042792563441026 26-27 0.1014947434946256 28-29 0.09421851647054347 30-31 0.10463669381041787 32-33 0.10227377386992811 34-35 0.09893685766341845 36-37 0.10569567520828899 38-39 0.09587572654164153 40-41 0.1054415530003026 42-43 0.08552087145687196 44-45 0.35316321409569396 46-47 0.07395706121214093 48-49 0.07123337105506725 50-51 0.06550687290591845 52-53 0.23371744446642395 54-55 0.26449622638519393 56-57 0.20663051665777737 58-59 0.26937703915366673 60-61 0.08032594697557663 62-63 0.06696161839819458 64-65 0.1077894755645833 66-67 0.10048325378808311 68-69 0.09289041548847696 70-71 0.10187051108741858 72-73 0.1268186464111437 74-75 0.1084551924307509 76-77 0.10058573585884482 78-79 0.0954541336326543 80-81 0.09377859343507854 82-83 0.10109564664995192 84-85 0.09533165506028053 86-87 0.11719533001034801 88-89 0.127270234072549 90-91 0.14636189398664703 92-93 0.15016539523077105 94-95 0.11201040377985871 96-97 0.09760625711865042 98-99 0.11572975307969871 100-101 0.11223036529759119 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6.0010497E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.117919028100797 #Duplication Level Percentage of deduplicated Percentage of total 1 78.45069204503717 16.567153623055663 2 7.994583603207536 3.3765793839183815 3 3.121823194450347 1.977792283213482 4 1.855158331731073 1.5670833373521345 5 1.228988518391838 1.2976840008932202 6 0.912951427900863 1.156778059859966 7 0.6860252386838839 1.014119780923185 8 0.5435283452050761 0.9182550066814732 9 0.4492534666191039 0.8538568498040239 >10 3.7429657774290614 16.30585478942662 >50 0.5384137664670372 7.9858180966672005 >100 0.40987839283124505 16.921361267923768 >500 0.03626720993984984 5.275784108353959 >1k 0.024503832696982975 10.294116947336574 >5k 0.0029155009688143605 4.26400523496073 >10k+ 0.002051348440482281 10.223757229630012 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 153622 0.25599188088710545 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 152493 0.2541105433604391 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 145170 0.24190767825168985 No Hit GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT 131237 0.21869007350497363 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 127955 0.21322103031407988 No Hit CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA 107554 0.17922531119847251 No Hit GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT 90418 0.15067030689647512 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 83515 0.13916731934414742 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 80007 0.13332167537289352 No Hit ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT 68448 0.11406004519509312 No Hit CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC 68000 0.11331350913491019 No Hit CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT 66505 0.11082227830907648 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 63677 0.1061097694291717 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 63236 0.10537489799492912 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 60851 0.10140059329953557 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01957157595278706 0.0 0.0 6.665500537347658E-6 3.332750268673829E-6 2 0.01985652610075867 0.0 0.0 1.16646259403584E-5 3.332750268673829E-6 3 0.020006499862848994 0.0 0.0 1.16646259403584E-5 3.332750268673829E-6 4 0.021134635828795087 1.6663751343369144E-6 0.0 1.16646259403584E-5 3.332750268673829E-6 5 0.021367928347602254 1.6663751343369144E-6 0.0 1.16646259403584E-5 3.332750268673829E-6 6 0.021642880244767845 1.6663751343369144E-6 0.0 1.499737620903223E-5 3.332750268673829E-6 7 0.02181285050847021 1.6663751343369144E-6 0.0 2.33292518807168E-5 3.332750268673829E-6 8 0.022679365578325405 1.6663751343369144E-6 0.0 2.33292518807168E-5 3.332750268673829E-6 9 0.023030970731670493 1.6663751343369144E-6 0.0 2.8328377283727545E-5 3.332750268673829E-6 10-11 0.024001634247421747 3.332750268673829E-6 0.0 2.8328377283727545E-5 3.332750268673829E-6 12-13 0.025769658264953213 3.332750268673829E-6 0.0 3.1661127552401374E-5 5.8323129701792E-6 14-15 0.027591839474350628 3.332750268673829E-6 0.0 3.4160690253906744E-5 6.665500537347658E-6 16-17 0.029871440658123527 3.332750268673829E-6 0.0 3.915981565691749E-5 6.665500537347658E-6 18-19 0.03216603921810546 4.999125403010743E-6 0.0 4.999125403010743E-5 6.665500537347658E-6 20-21 0.03450646309428165 7.498688104516114E-6 0.0 5.998950483612892E-5 6.665500537347658E-6 22-23 0.0368352223445175 1.0831438373189943E-5 0.0 6.332225510480275E-5 9.998250806021486E-6 24-25 0.0390565003985886 1.16646259403584E-5 0.0 7.082094320931887E-5 1.4164188641863772E-5 26-27 0.041331102456958486 1.16646259403584E-5 0.0 9.998250806021486E-5 1.499737620903223E-5 28-29 0.0436157027661344 1.16646259403584E-5 0.0 1.1248032156774172E-4 1.499737620903223E-5 30-31 0.04605194321253497 1.16646259403584E-5 0.0 1.2331175994093166E-4 1.499737620903223E-5 32-33 0.04832154614550185 1.3331001074695315E-5 0.0 1.291440729111109E-4 1.499737620903223E-5 34-35 0.05107356467985926 1.6663751343369143E-5 0.0 1.3497638588129006E-4 1.499737620903223E-5 36-37 0.05362645138566341 1.7496938910537598E-5 0.0 1.4247507398580618E-4 1.499737620903223E-5 38-39 0.05630764897681151 1.9996501612042972E-5 0.0 1.4664101182164846E-4 1.499737620903223E-5 40-41 0.05921797314893093 2.1662876746379887E-5 0.0 1.5247332479182768E-4 1.499737620903223E-5 42-43 0.0623674221528277 2.4995627015053716E-5 0.0 1.633047631650176E-4 1.6663751343369143E-5 44-45 0.06598178981920447 2.666200214939063E-5 0.0 1.6747070100085992E-4 1.6663751343369143E-5 46-47 0.07007357396156876 2.8328377283727545E-5 0.0 1.7663576423971293E-4 1.7496938910537598E-5 48-49 0.07439531787247154 2.999475241806446E-5 0.0 1.8163488964272368E-4 1.8330126477706057E-5 50-51 0.07923113851231728 3.0827939985232915E-5 0.0 1.8663401504573444E-4 1.8330126477706057E-5 52-53 0.08456187256706105 3.3327502686738285E-5 0.0 1.9579907828458744E-4 1.8330126477706057E-5 54-55 0.09043751129073302 3.3327502686738285E-5 0.0 2.0496414152344048E-4 1.8330126477706057E-5 56-57 0.09683639180658676 3.5827065388243656E-5 0.0 2.1329601719512507E-4 1.9163314044874516E-5 58-59 0.10397347650695177 3.6660252955412115E-5 0.0 2.2329426800114652E-4 1.9996501612042972E-5 60-61 0.11166629731461813 3.832662808974903E-5 0.0 2.299597685384942E-4 1.9996501612042972E-5 62-63 0.12028728907211017 4.165937835842286E-5 0.0 2.4079120691168415E-4 1.9996501612042972E-5 64-65 0.130292205378669 4.165937835842286E-5 0.0 2.53289020419211E-4 1.9996501612042972E-5 66-67 0.1424825726739107 4.332575349275977E-5 0.0 2.5995452095655867E-4 2.0829689179211428E-5 68-69 0.15554362097684343 4.415894105992823E-5 0.0 2.649536463595694E-4 2.1662876746379887E-5 70-71 0.17017689421902304 4.582531619426515E-5 0.0 2.691195841954117E-4 2.1662876746379887E-5 72-73 0.18624491645186675 5.332400429878126E-5 0.0 2.724523344640855E-4 2.33292518807168E-5 74-75 0.20544739031239817 5.415719186594972E-5 0.0 2.841169604044439E-4 2.33292518807168E-5 76-77 0.2278093114276324 5.499037943311818E-5 0.0 2.999475241806446E-4 2.33292518807168E-5 78-79 0.25313904665712067 5.499037943311818E-5 0.0 3.0827939985232915E-4 2.33292518807168E-5 80-81 0.2825422359024955 5.499037943311818E-5 8.331875671684572E-7 3.266095263300352E-4 2.33292518807168E-5 82-83 0.3178852193142143 5.748994213462355E-5 1.6663751343369144E-6 3.424400901062359E-4 2.33292518807168E-5 84-85 0.35965541161907055 5.832312970179201E-5 1.6663751343369144E-6 3.5077196577792047E-4 2.4995627015053716E-5 86-87 0.41201958384047377 5.915631726896047E-5 1.6663751343369144E-6 3.641029668526158E-4 2.666200214939063E-5 88-89 0.4735146586104761 5.998950483612892E-5 1.6663751343369144E-6 3.682689046884581E-4 2.666200214939063E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 44110 0.0 55.553444 1 GCGTGTT 60090 0.0 40.469017 2 GTTTTTT 623240 0.0 35.38248 1 TCGTCTA 52480 0.0 31.341473 44-45 CGTGTTT 78810 0.0 31.272604 3 ACTCGTC 54520 0.0 30.264688 42-43 CTAACGA 57705 0.0 27.453419 94-95 TTAGGCA 53720 0.0 27.307728 8 CCTTTAG 55105 0.0 26.872932 5 TAGGCAT 56015 0.0 26.095448 9 GTCTATG 67105 0.0 24.727562 46-47 CAAAACG 39625 0.0 24.31745 9 ATGTGGC 68620 0.0 24.30166 50-51 GCCTAAC 67075 0.0 23.947247 92-93 CCCGAAA 68040 0.0 23.935232 1 CTATGTG 72165 0.0 23.113089 48-49 CCCGTAT 5995 0.0 22.757017 1 GAGGTGA 70915 0.0 22.223928 82-83 TTTAGGC 70720 0.0 22.192436 7 CACCAAT 55235 0.0 22.065563 1 >>END_MODULE