FastQCFastQC Report
Fri 27 May 2016
SRR1233033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60010497
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1515550.25254748348443107No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1415960.23595205352156973No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1277570.21289108803748116No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA996990.16613593451825603No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC995190.1658359869940754No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT992250.16534607270458035No Hit
CACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCA946930.15779406059576545No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT756340.12603461691043819No Hit
ATACGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACT675270.11252531369636881No Hit
CGACTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCAC645010.10748286253986532No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC641030.10681964523639922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT427700.055.214831
GCGTGTT578600.040.4260642
CGTGTTT757200.031.2869473
TCGTCTA514150.031.04327444-45
GTTTTTT5947950.030.9587481
ACTCGTC538950.029.73836342-43
TCGACTC41800.027.5940993
CTAACGA567750.027.12450894-95
TTAGGCA503750.025.2682578
CCCGAAA602350.024.723141
GTCTATG651750.024.63109246-47
TAGGCAT522550.024.2867249
CCTTTAG526350.024.151475
ATGTGGC678400.023.58571450-51
GCCTAAC654800.023.45401892-93
CTATGTG704050.022.96286248-49
CGCGTTT31650.021.9056261
GAGGTGA710150.021.81996582-83
CCCGTAT54950.021.6911471
CAAAACG335250.021.2715429
Produced by FastQC (version 0.11.5)