FastQCFastQC Report
Fri 27 May 2016
SRR1233032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71406128
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA2949270.4130275765687785No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA2076560.2908097747577071No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA2062610.2888561609166093No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT2049740.2870537945986933No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1947550.2727426979376336No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG1639320.22957693490956405No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC1591760.22291644212944858No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1558580.21826978211169779No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT1460550.20454126850289375No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1448490.20285233782736406No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA1407170.19706571962563213No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1338300.18742088914273575No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1228230.172006245738461No Hit
CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC1191760.16689884095101754No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1167030.16343555275816105No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT1078670.1510612646578456No Hit
TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA1043120.14608270035311255No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC1036440.14514720641343276No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1008510.1412357774111488No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT981440.13744478625139847No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT934470.1308669194330212No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT927300.12986280393189784No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT873840.12237605153440052No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT873370.12231023085301587No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT858850.12027679193023881No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA843620.11814392176537004No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA836350.11712580186395206No Hit
CTTTAATACGACTCACTATAGGGACTTTAATACGACTCACTATAGGGACT807710.1131149416195764No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT791360.11082522217140804No Hit
ATACGACTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCA767800.10752578546199844No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT763010.10685497468788674No Hit
CGACTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTA749450.10495597800793792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTT451850.048.032842
GTTTTTT7599000.043.9207461
CAAAACG520300.043.210019
CGTCGTA103100.040.363441
CGCGTGT595200.037.329071
CCTTTAG691150.035.718065
CCGAAAC890650.035.480351
CGTGTTT630750.034.5520363
TTAGGCA710800.034.1487778
TAACGAG557200.034.0700894-95
TAGGCAT727400.033.526199
TCGTCTA725300.032.12444344-45
TAACGGC61100.032.10789
ACTCGTC726800.032.06990442-43
TTTAGGC789850.031.4948547
CTAACGA684100.031.39686894-95
CGAAACC1012450.031.0736712
CTTTGCT836300.030.0778371
CCAAACG1053300.029.958077
TCCTTTA856650.029.388674
Produced by FastQC (version 0.11.5)