FastQCFastQC Report
Fri 27 May 2016
SRR1233032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71406128
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA2881120.4034835777680033No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1999560.2800263865308591No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1846720.25862206112058056No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1788680.25049390718959025No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG1535120.2149843498025828No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1487140.20826503854122994No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1456290.20394468105034347No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT1436030.20110738955065594No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1380990.19339936762850382No Hit
ATACGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGA1347800.1887513071707235No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1334820.1869335360124834No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC1277360.17888660760320177No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1248670.1748687451586788No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT1207890.16915775071853778No Hit
TTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACAA1102500.15439851324805065No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1091900.15291404681682222No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1083560.15174607983225194No Hit
CGACTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTC1081360.15143798302577055No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC998510.13983533738168802No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT969690.13579926921678206No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT950210.13307121203939248No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT892250.1249542616286378No Hit
ATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTATTACA877570.1228984156653894No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT876520.122751369462296No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA852270.11935530239085361No Hit
ATACGACTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCA803630.1125435620875564No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT800790.11214583711918955No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT790550.11071178652902172No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT754330.10563939274231476No Hit
CGACTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTA715310.10017487574735882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTT456850.049.3677862
GTTTTTT7414900.043.764221
CAAAACG458950.039.7549829
CGCGTGT579950.039.5925941
CCGAAAC846000.036.887511
CGTCGTA113400.035.7251321
CGTGTTT641050.035.3527073
CCTTTAG620450.034.3594065
TAACGAG540250.033.2804794-95
CGAAACC969150.032.236932
ACTCGTC726750.031.827342-43
TTAGGCA663650.031.7873948
TCGTCTA725750.031.78674744-45
CCAAACG1009100.031.1154617
TAGGCAT677550.031.0861519
TAACGGC61300.031.0739569
CTAACGA672200.031.01398394-95
CCCGAAA523550.030.44341
CAAACGA1072300.029.1135048
TTTAGGC750850.028.8862867
Produced by FastQC (version 0.11.5)