FastQCFastQC Report
Fri 27 May 2016
SRR1233031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87980627
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA6398260.7272350991542718No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA3570680.4058484375202282No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT3312700.37652607317745074No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC3198970.36359936375538676No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT2438120.2771200982689064No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2308700.2624100417015669Illumina Single End PCR Primer 1 (100% over 50bp)
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT1971060.22403341135543398No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1785460.20293785812642595No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1399460.15906456315661402No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1381510.15702434127913184No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC1039460.11814646422103811No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG977750.11113242009516483No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT976140.11094942526381404No Hit
CTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATT963920.10956048312772311No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT917690.10430591725607957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT562100.067.1216051
GCGTGTT690350.053.929962
AGAGCGT707700.047.1170589
AAGAGCG767550.044.4394578
ATCGGAA823600.042.637443
GATCGGA835550.042.0283622
GTTTTTT12211850.041.7376371
AGATCGG847550.041.2420731
CCTTTAG1013350.041.1081925
CTCGCAT149000.040.8102951
CGGAAGA852500.039.871655
TCGGAAG872450.039.6130874
CGCCGTA457850.038.40854346-47
CCGAAAC1827150.038.3524631
CCGTATC460600.038.31289748-49
GTCGCCG470800.037.44302444-45
CGTGTTT1025100.036.5731743
TTAGGCA1138050.036.2659388
TAACGAG1121350.035.4295694-95
CAAAACG473400.035.118029
Produced by FastQC (version 0.11.5)