##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233031_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87980627 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.001177679718058 31.0 30.0 33.0 25.0 34.0 2 30.3884727032009 31.0 31.0 34.0 26.0 34.0 3 30.268147429774512 31.0 31.0 34.0 26.0 34.0 4 33.5449417631452 37.0 35.0 37.0 27.0 37.0 5 33.39380943488843 37.0 35.0 37.0 26.0 37.0 6 33.394839798084185 37.0 35.0 37.0 26.0 37.0 7 33.65683550993561 37.0 35.0 37.0 28.0 37.0 8 33.54937456856269 37.0 35.0 37.0 28.0 37.0 9 34.83563935046746 38.0 35.0 39.0 27.0 39.0 10-11 34.49797400852803 38.0 34.0 39.0 26.5 39.0 12-13 34.388827093719165 38.0 34.0 39.0 26.0 39.0 14-15 35.1939370413898 38.0 34.0 40.0 25.0 41.0 16-17 35.14612899382952 38.0 34.0 40.0 25.0 41.0 18-19 34.884864664581215 38.5 34.0 40.0 24.5 41.0 20-21 34.58619554961798 38.0 34.0 40.0 22.5 41.0 22-23 34.25220764225743 38.0 33.5 40.0 18.5 41.0 24-25 33.967883378462396 38.0 33.0 40.0 17.5 41.0 26-27 33.60035247305069 38.0 33.0 40.0 15.0 41.0 28-29 33.29054594598422 38.0 32.5 40.0 10.0 41.0 30-31 33.04887360600419 38.0 32.0 40.0 9.5 41.0 32-33 32.81642416574276 38.0 32.0 40.0 9.0 41.0 34-35 32.559260619954436 38.0 31.0 40.0 8.0 41.0 36-37 32.316516464471206 38.0 31.0 40.0 7.5 41.0 38-39 32.16106129250477 38.0 30.0 40.0 2.0 41.0 40-41 32.00072954128868 38.0 30.0 40.0 2.0 41.0 42-43 31.806145863225094 38.0 30.0 40.0 2.0 41.0 44-45 31.627100537712693 38.0 30.0 40.0 2.0 41.0 46-47 31.37885644984094 37.0 30.0 40.0 2.0 41.0 48-49 31.147068439282663 37.0 30.0 40.0 2.0 41.0 50-51 30.575024016366694 36.5 28.5 39.5 2.0 40.5 52-53 30.574964190696207 37.0 29.0 39.0 2.0 40.0 54-55 30.760295672818973 37.0 29.0 40.0 2.0 40.5 56-57 30.557708295259136 37.0 28.0 40.0 2.0 41.0 58-59 30.27013425921595 36.0 28.0 39.5 2.0 41.0 60-61 30.001199519753364 36.0 28.0 39.0 2.0 41.0 62-63 29.737141700524596 36.0 27.0 39.0 2.0 40.0 64-65 29.406620630243975 35.5 26.0 39.0 2.0 40.0 66-67 29.076667565690343 35.0 26.0 39.0 2.0 40.0 68-69 28.648185139667167 35.0 26.0 38.0 2.0 40.0 70-71 28.211766415349597 34.0 25.0 38.0 2.0 40.0 72-73 27.771434619350917 34.0 23.5 37.5 2.0 39.5 74-75 27.298011231495316 34.0 22.0 37.0 2.0 39.0 76-77 26.842885337700537 33.5 22.0 36.0 2.0 39.0 78-79 26.300976475196066 33.0 20.5 36.0 2.0 38.5 80-81 25.782366838554125 32.0 18.5 35.0 2.0 37.5 82-83 25.305166772680536 32.0 17.5 35.0 2.0 37.0 84-85 24.79388044711252 31.5 10.5 35.0 2.0 36.5 86-87 24.20545710023185 31.0 7.0 34.5 2.0 36.0 88-89 23.70223339053949 31.0 3.5 34.0 2.0 36.0 90-91 23.210413424309877 31.0 2.0 34.0 2.0 35.0 92-93 22.63132065426176 30.0 2.0 34.0 2.0 35.0 94-95 21.960218929787803 30.0 2.0 34.0 2.0 35.0 96-97 21.146324337970448 29.0 2.0 34.0 2.0 35.0 98-99 20.027434011126108 29.0 2.0 34.0 2.0 35.0 100-101 16.18401996612277 13.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46523230623073175 1101 2 0.391251893029974 1101 3 0.44001739108820104 1101 4 0.5208071330003889 1101 5 0.5148657060972397 1101 6 0.4560539823121701 1101 7 0.47686558214218877 1101 8 0.5381104187785795 1101 9 0.5883825312585316 1101 10-11 0.7152007024823277 1101 12-13 0.7200658908828785 1101 14-15 0.7636651370092977 1101 16-17 0.7117899064039861 1101 18-19 0.7428095294467667 1101 20-21 0.7655676967744967 1101 22-23 0.8406729892593248 1101 24-25 0.7797388402672425 1101 26-27 0.6524589571809187 1101 28-29 0.7186065773425554 1101 30-31 0.7945936098619768 1101 32-33 0.8224899287681922 1101 34-35 0.8445711217760135 1101 36-37 0.8626067150382397 1101 38-39 0.9078820582432883 1101 40-41 0.8766618582053596 1101 42-43 0.9904747071866673 1101 44-45 0.9881155565170054 1101 46-47 0.7861558787825444 1101 48-49 0.8919299403924583 1101 50-51 0.7629124295637517 1101 52-53 0.7371394585936741 1101 54-55 0.918928393921405 1101 56-57 0.9553821055936034 1101 58-59 0.9922456714597416 1101 60-61 1.04879085109161 1101 62-63 1.027987175788077 1101 64-65 1.1479401342782758 1101 66-67 1.123654911827309 1101 68-69 1.203150992449963 1101 70-71 1.1563115927131626 1101 72-73 1.12195532319857 1101 74-75 1.209357611601252 1101 76-77 1.2018736833642265 1101 78-79 1.2366025834220338 1101 80-81 1.3317633619129055 1101 82-83 1.4153593092578198 1101 84-85 1.370438363561643 1101 86-87 1.442424663676789 1101 88-89 1.5025263493543548 1101 90-91 1.5968371089521085 1101 92-93 1.5909643914052474 1101 94-95 1.654236518457818 1101 96-97 1.8142911047562578 1101 98-99 1.9641948421562248 1101 100-101 2.6993877096393284 1102 1 0.40942401038788034 1102 2 0.2978192481374329 1102 3 0.3523576727147173 1102 4 0.4526343750953501 1102 5 0.43579295830272 1102 6 0.4984848838813605 1102 7 0.4411214409276738 1102 8 0.5476062092084035 1102 9 0.5892548509583548 1102 10-11 0.5766161353343975 1102 12-13 0.5867680238459556 1102 14-15 0.5845669608349908 1102 16-17 0.5640710361522494 1102 18-19 0.4878511630522837 1102 20-21 0.6169969763487373 1102 22-23 0.655976759901101 1102 24-25 0.6510157567265651 1102 26-27 0.7503572095841875 1102 28-29 0.6265739487645234 1102 30-31 0.6443110029328807 1102 32-33 0.6918835318148453 1102 34-35 0.733462090197385 1102 36-37 0.7899635437449604 1102 38-39 0.7773005454419177 1102 40-41 0.7932736875606281 1102 42-43 0.7720620553220705 1102 44-45 0.7583198112666842 1102 46-47 0.8655132663772562 1102 48-49 0.9060917809815052 1102 50-51 0.8501445800522625 1102 52-53 0.8185079733851381 1102 54-55 0.8519054729955791 1102 56-57 0.9277943723144659 1102 58-59 0.9406672521731672 1102 60-61 0.9385803049815706 1102 62-63 0.9781363958570779 1102 64-65 1.0545912140711806 1102 66-67 1.0952050941728118 1102 68-69 1.0496044692184299 1102 70-71 1.0673449165337203 1102 72-73 1.0962440684381534 1102 74-75 1.1620396925381087 1102 76-77 1.1318914726968288 1102 78-79 1.1928855538638388 1102 80-81 1.362444061872381 1102 82-83 1.42173070493644 1102 84-85 1.435479589774122 1102 86-87 1.4572276642396318 1102 88-89 1.4463702682156452 1102 90-91 1.6049607007039342 1102 92-93 1.5946899702990862 1102 94-95 1.5918452939659105 1102 96-97 1.6614849885087786 1102 98-99 1.8255559904032665 1102 100-101 2.6084424124420167 1103 1 0.39088008649967065 1103 2 0.34968467805661874 1103 3 0.3232882803609556 1103 4 0.4250837425321805 1103 5 0.3763924977212554 1103 6 0.41435236254104524 1103 7 0.4273587083745838 1103 8 0.45565898976989416 1103 9 0.5737158089033088 1103 10-11 0.538521913340027 1103 12-13 0.5833921650100535 1103 14-15 0.6718746201596346 1103 16-17 0.6103693462729751 1103 18-19 0.6006573565140556 1103 20-21 0.5729359060743917 1103 22-23 0.6611502725164371 1103 24-25 0.7114059419606704 1103 26-27 0.6325478244200013 1103 28-29 0.634432878928898 1103 30-31 0.7398381569356332 1103 32-33 0.6829045680020371 1103 34-35 0.8005382318065912 1103 36-37 0.8478397733825034 1103 38-39 0.8724790966082736 1103 40-41 0.8203774905972168 1103 42-43 0.8798214514797742 1103 44-45 0.8376971575336718 1103 46-47 0.8354194662711834 1103 48-49 0.9335653406962479 1103 50-51 0.829278945386303 1103 52-53 0.8058590833653874 1103 54-55 0.9168132874310224 1103 56-57 1.0151714999096733 1103 58-59 1.0219153016928217 1103 60-61 0.975708825416131 1103 62-63 1.0096745885119276 1103 64-65 1.154272318306237 1103 66-67 1.1259430756847166 1103 68-69 1.0704422219940604 1103 70-71 1.130554335926849 1103 72-73 1.2411597314479437 1103 74-75 1.2683265152366516 1103 76-77 1.2429395564212804 1103 78-79 1.2294808319987354 1103 80-81 1.3622564437571363 1103 82-83 1.4901506732430185 1103 84-85 1.5014042169106538 1103 86-87 1.5373173159731301 1103 88-89 1.6215719612382031 1103 90-91 1.7129927261274567 1103 92-93 1.72052239829695 1103 94-95 1.7999367798901744 1103 96-97 1.947354776764417 1103 98-99 2.0741071440692807 1103 100-101 2.7568838782472227 1104 1 0.20392438813385994 1104 2 0.1386088392394491 1104 3 0.09635942347012261 1104 4 0.13413952461434064 1104 5 0.16721611680881665 1104 6 0.1867819521393841 1104 7 0.18381116556157195 1104 8 0.292550499792263 1104 9 0.3098878782358696 1104 10-11 0.3453443215306109 1104 12-13 0.32721314473974417 1104 14-15 0.4096094498140559 1104 16-17 0.3463738579505957 1104 18-19 0.2555381285246128 1104 20-21 0.2891183554203707 1104 22-23 0.33362975424856955 1104 24-25 0.3294121657583702 1104 26-27 0.34886525568323634 1104 28-29 0.3380296540088068 1104 30-31 0.36103744928449544 1104 32-33 0.413354584288264 1104 34-35 0.395408029643157 1104 36-37 0.3761195295131188 1104 38-39 0.38299420556627695 1104 40-41 0.49306379437932435 1104 42-43 0.49753536906582596 1104 44-45 0.5238054039710285 1104 46-47 0.5391664695238276 1104 48-49 0.5373658169417688 1104 50-51 0.4348554755420224 1104 52-53 0.5046832664741672 1104 54-55 0.6033023057960172 1104 56-57 0.6139007558667693 1104 58-59 0.6032243846209404 1104 60-61 0.6061834985150831 1104 62-63 0.6639443600039385 1104 64-65 0.8050894618906099 1104 66-67 0.7926358941843752 1104 68-69 0.7294985440730706 1104 70-71 0.7431810693334633 1104 72-73 0.869740289399541 1104 74-75 0.9351668426620456 1104 76-77 0.9170690421219376 1104 78-79 1.045492523946205 1104 80-81 1.1014271125526562 1104 82-83 1.1930612110634016 1104 84-85 1.1537343870030732 1104 86-87 1.2682904897128005 1104 88-89 1.2954463834348715 1104 90-91 1.3665973517167842 1104 92-93 1.4477010432709392 1104 94-95 1.5030279994752753 1104 96-97 1.5863922900479857 1104 98-99 1.6143603733093173 1104 100-101 2.163257211417509 1105 1 -0.032699194289847355 1105 2 -0.02853570612674261 1105 3 -0.027260887647898357 1105 4 0.03233746351930478 1105 5 0.06536128929991492 1105 6 0.07594493688246473 1105 7 0.026104601833431218 1105 8 0.09782918705987242 1105 9 0.10117545885418622 1105 10-11 0.12469668144263579 1105 12-13 0.06714037716052701 1105 14-15 0.056037382809563496 1105 16-17 0.032687963404974596 1105 18-19 0.027076749080933382 1105 20-21 -8.159375799579038E-4 1105 22-23 0.06871020143046991 1105 24-25 0.09402948454628302 1105 26-27 0.062080296967025106 1105 28-29 0.017159460147155414 1105 30-31 0.06640524052592411 1105 32-33 0.1237231475108942 1105 34-35 0.08380226666158563 1105 36-37 0.16933436258261736 1105 38-39 0.13236973816842124 1105 40-41 0.20611829537018878 1105 42-43 0.16358020668021211 1105 44-45 0.21361372387626787 1105 46-47 0.2537378675271995 1105 48-49 0.2960973232795503 1105 50-51 0.1933709738474434 1105 52-53 0.2098878591960336 1105 54-55 0.22875627900295825 1105 56-57 0.25196984313286563 1105 58-59 0.22990490274581532 1105 60-61 0.3069946607029834 1105 62-63 0.3988851218620937 1105 64-65 0.4266906516091602 1105 66-67 0.4349941310559515 1105 68-69 0.38984919454253486 1105 70-71 0.436959752831374 1105 72-73 0.4231322836515474 1105 74-75 0.5271856939250696 1105 76-77 0.5630001216155094 1105 78-79 0.6310837564680369 1105 80-81 0.6995765316073488 1105 82-83 0.725288188527113 1105 84-85 0.6622785519345449 1105 86-87 0.7621064040254701 1105 88-89 0.7596803787152027 1105 90-91 0.9117937719905704 1105 92-93 1.0235097770193065 1105 94-95 1.1377853486662062 1105 96-97 1.2908072458519833 1105 98-99 1.4796958944616243 1105 100-101 2.171042267151826 1106 1 -0.47109073643245836 1106 2 -0.5053829125542997 1106 3 -0.5920852273989325 1106 4 -0.5616634581868354 1106 5 -0.5433697101924508 1106 6 -0.5939251744080778 1106 7 -0.541309918493539 1106 8 -0.5991692573954381 1106 9 -0.7476650700960192 1106 10-11 -0.7370018197438881 1106 12-13 -0.7493813147758388 1106 14-15 -0.7026380164687964 1106 16-17 -0.7643614808015684 1106 18-19 -0.6976262425690507 1106 20-21 -0.7474783699329066 1106 22-23 -0.7206900766778475 1106 24-25 -0.6666582576648352 1106 26-27 -0.7193163933068831 1106 28-29 -0.8251297923130991 1106 30-31 -0.7012410933601956 1106 32-33 -0.7320133724039621 1106 34-35 -0.6789655895816722 1106 36-37 -0.639524044606091 1106 38-39 -0.6178946791448254 1106 40-41 -0.7246629362054868 1106 42-43 -0.8439161824189689 1106 44-45 -0.7123763307689224 1106 46-47 -0.7140163838813436 1106 48-49 -0.6766010590501992 1106 50-51 -0.7128167604799849 1106 52-53 -0.7651279515165292 1106 54-55 -0.6361634517431263 1106 56-57 -0.7511985483801205 1106 58-59 -0.6373334066826501 1106 60-61 -0.6784050085323443 1106 62-63 -0.5658233981904992 1106 64-65 -0.4994908744628681 1106 66-67 -0.5792385228920836 1106 68-69 -0.6529024177551861 1106 70-71 -0.6307297524947018 1106 72-73 -0.38212227785643194 1106 74-75 -0.2847014498234621 1106 76-77 -0.26030235571810323 1106 78-79 -0.19168626264911381 1106 80-81 -0.16274355768471693 1106 82-83 -0.11618429787993634 1106 84-85 -0.04664290770120871 1106 86-87 0.10306279485401504 1106 88-89 0.10060070460903958 1106 90-91 0.2470188574605423 1106 92-93 0.27283429599439657 1106 94-95 0.33854090062774134 1106 96-97 0.4851190307286828 1106 98-99 0.6441230986796747 1106 100-101 1.4154779133844002 1108 1 -2.052332186739772 1108 2 -2.0273301832080257 1108 3 -2.047127084797058 1108 4 -2.2270973354757864 1108 5 -2.6015717908275597 1108 6 -2.559817188936197 1108 7 -2.457394339391282 1108 8 -3.0355888189566294 1108 9 -3.226768900377806 1108 10-11 -3.3295823829057554 1108 12-13 -3.234875403057707 1108 14-15 -3.713395332917365 1108 16-17 -3.409991257245437 1108 18-19 -3.4349261437346854 1108 20-21 -3.679948605313573 1108 22-23 -3.8114851320721463 1108 24-25 -3.6726328574366462 1108 26-27 -3.4643610022429137 1108 28-29 -3.204570035563542 1108 30-31 -3.0353351096357386 1108 32-33 -3.1178766191356537 1108 34-35 -3.85897681135933 1108 36-37 -3.983122099254917 1108 38-39 -3.40806280403509 1108 40-41 -3.11297096086626 1108 42-43 -3.0774511795610415 1108 44-45 -2.9844317383684107 1108 46-47 -2.9969878366422904 1108 48-49 -3.090175628119777 1108 50-51 -3.109972947821454 1108 52-53 -3.008687389660082 1108 54-55 -3.104062193762953 1108 56-57 -3.2679650454482463 1108 58-59 -3.204140498639191 1108 60-61 -3.4204143947187546 1108 62-63 -3.2157564035212545 1108 64-65 -3.0054815764957716 1108 66-67 -2.961892232039947 1108 68-69 -3.1156305852353867 1108 70-71 -2.9701194900487877 1108 72-73 -2.9177839465313653 1108 74-75 -2.939814219213826 1108 76-77 -2.858384805447791 1108 78-79 -3.1144829071775817 1108 80-81 -3.111804097370044 1108 82-83 -2.758840831910529 1108 84-85 -2.749081962502583 1108 86-87 -2.6597796779080625 1108 88-89 -2.7312348617953646 1108 90-91 -2.871861163175403 1108 92-93 -2.9050603958329297 1108 94-95 -2.8211860257142014 1108 96-97 -2.7196656763130385 1108 98-99 -2.6359965651283375 1108 100-101 -1.9591576673640443 1201 1 0.6141876701927522 1201 2 0.5897268495501642 1201 3 0.647033807963826 1201 4 0.7115373141072396 1201 5 0.7499513068987937 1201 6 0.67491874802419 1201 7 0.6704709391767523 1201 8 0.6900131831759921 1201 9 0.7908245986319926 1201 10-11 0.7561878721297077 1201 12-13 0.7618811321058274 1201 14-15 0.8651280592164738 1201 16-17 0.893159753605623 1201 18-19 0.8377732459494922 1201 20-21 0.9423500117614907 1201 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2304 76-77 0.3594192372424523 2304 78-79 0.3415799119474201 2304 80-81 0.38576796277262915 2304 82-83 0.5313898578373362 2304 84-85 0.5277159409677239 2304 86-87 0.5364175977801793 2304 88-89 0.6403254075244504 2304 90-91 0.5975363247058034 2304 92-93 0.5010303174917254 2304 94-95 0.4219305994614473 2304 96-97 0.5090184439696408 2304 98-99 0.6669018631475296 2304 100-101 0.6006378514813449 2305 1 -0.20154585126125113 2305 2 -0.2755797537762632 2305 3 -0.2992354126832808 2305 4 -0.2979202249654449 2305 5 -0.23278836846501605 2305 6 -0.21660206731322518 2305 7 -0.2599721440472962 2305 8 -0.3571321373286622 2305 9 -0.30165502273035827 2305 10-11 -0.3565883846596307 2305 12-13 -0.3629581818237284 2305 14-15 -0.4128235608287696 2305 16-17 -0.38638275665192623 2305 18-19 -0.36891114194512653 2305 20-21 -0.3778410530013687 2305 22-23 -0.46910589529716873 2305 24-25 -0.45496203153855674 2305 26-27 -0.2978725091896308 2305 28-29 -0.31469079966694125 2305 30-31 -0.31748594537022257 2305 32-33 -0.38536019113539766 2305 34-35 -0.288786042737712 2305 36-37 -0.3699616489618549 2305 38-39 -0.3696181153043483 2305 40-41 -0.3538071247411523 2305 42-43 -0.46421817003847465 2305 44-45 -0.3933723019085029 2305 46-47 -0.275163909459895 2305 48-49 -0.2933092373973132 2305 50-51 -0.2583447075045271 2305 52-53 -0.3071226278082335 2305 54-55 -0.4068063233441954 2305 56-57 -0.3598319695126371 2305 58-59 -0.42191741165365926 2305 60-61 -0.4270545608036187 2305 62-63 -0.31126624231820443 2305 64-65 -0.2590466913981757 2305 66-67 -0.22462546424065977 2305 68-69 -0.17001367016290203 2305 70-71 -0.2124481001439591 2305 72-73 -0.32053165844944687 2305 74-75 -0.21859049945050302 2305 76-77 -0.25797555705318587 2305 78-79 -0.15874282992248467 2305 80-81 -0.10424597280184145 2305 82-83 -0.019908766795712296 2305 84-85 -0.06308923507063113 2305 86-87 -0.12891224032510706 2305 88-89 -0.049079916717914784 2305 90-91 -0.05646569777899657 2305 92-93 -0.054503767867835506 2305 94-95 -0.008029801086838262 2305 96-97 0.06850665291342395 2305 98-99 0.05903956552941736 2305 100-101 -0.08832998968903283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1540636.0 3 432515.0 4 403732.0 5 432473.0 6 543429.0 7 765243.0 8 1075734.0 9 1386958.0 10 1413256.0 11 1226087.0 12 999425.0 13 832161.0 14 742648.0 15 703398.0 16 701961.0 17 720294.0 18 756540.0 19 807842.0 20 873037.0 21 953903.0 22 1048223.0 23 1158751.0 24 1272565.0 25 1387059.0 26 1556915.0 27 1768659.0 28 2025406.0 29 2340052.0 30 2742324.0 31 3246388.0 32 3915774.0 33 4806886.0 34 6040981.0 35 7795772.0 36 1.0204974E7 37 1.1992381E7 38 6778151.0 39 588034.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.56535160874013 16.34490848543378 11.115086201452716 47.974653704373374 2 13.144042904308998 17.26279923602845 56.48572837581801 13.107429483844543 3 16.332101718776947 23.32285828431557 39.84048474919526 20.50455524771222 4 18.642411744589086 30.958023934623203 32.976328526531525 17.42323579425619 5 18.36592083523906 33.607962940904216 33.04189470946764 14.984221514389088 6 15.023629611912739 36.392854202676304 33.12629843407227 15.457217751338687 7 12.742200016351344 18.352912531097644 51.6271127101668 17.277774742384217 8 13.055883029566772 21.578866249358946 44.2121172304928 21.15313349058148 9 15.2544590265355 23.501138927696775 41.764882427119396 19.47951961864833 10-11 19.298162441617475 34.89451865568878 30.472677751788975 15.33464115090477 12-13 17.874282388480108 25.952376871385518 38.397595637264764 17.775745102869614 14-15 18.735287562863224 27.621629855968443 37.34375965682505 16.299322924343286 16-17 18.665355925295792 27.09892788254421 36.29465554371015 17.94106064844985 18-19 19.193237922400037 28.784107581158697 34.49963766579241 17.523016830648853 20-21 19.044049806945957 28.180207302469295 34.55907186070545 18.216671029879297 22-23 19.307829118210034 30.672092993359122 33.6787420093522 16.341335879078645 24-25 18.129129501926023 30.931871555310124 33.14062797935866 17.798370963405187 26-27 18.22150468899678 30.992641659896226 32.6160070352001 18.1698466159069 28-29 18.318278480201197 31.244130835059153 32.35881397275413 18.078776711985522 30-31 17.799142592761157 30.36534712225568 33.505031647389465 18.330478637593693 32-33 18.178616639972823 30.382233366328588 33.455984982826166 17.98316501087243 34-35 18.694157632672688 31.871284359871428 30.959836584634594 18.47472142282129 36-37 19.081849153201688 31.36077361650449 31.73126322227276 17.826114008021065 38-39 18.707140511895762 30.546246377018655 32.097863463252196 18.648749647833387 40-41 19.475793962508032 30.62944540407009 31.151212816653256 18.743547816768626 42-43 19.059168996257224 31.16265257299545 31.71232423188044 18.06585419886688 44-45 19.73130250106984 30.15909063785266 31.485780063389157 18.62382679768835 46-47 19.42991537305389 29.492671959114436 31.981529253403906 19.095883414427767 48-49 19.30975585537903 29.90210414238637 31.337701359780155 19.45043864245444 50-51 18.222925703473106 30.367060832434074 33.05878593833761 18.351227525755217 52-53 19.557189802902144 30.473676105865295 32.006114032324554 17.96302005890801 54-55 19.543319454423614 30.522545145063635 31.180684209388502 18.753451191124253 56-57 19.572522778584037 30.79347434188619 30.816265229809247 18.817737649720527 58-59 18.838824704715794 31.612738060418316 30.636895064583765 18.911542170282125 60-61 19.135755688996845 31.804904007410595 30.23722962841699 18.822110675175573 62-63 19.367435808591857 32.41119491249684 30.53320489797696 17.68816438093435 64-65 19.811425409231155 31.22745925794777 30.632833142823124 18.328282189997953 66-67 19.414027067585284 32.169762501537484 29.874852394219843 18.54135803665739 68-69 19.539264272360082 31.874079040447416 30.41764696104126 18.169009726151238 70-71 19.884418329330813 31.63838358244984 29.952395298652135 18.52480278956721 72-73 18.748599690786268 31.593659977280435 31.010584064426588 18.647156267506706 74-75 18.65633594841782 30.022999766345844 32.84803901739135 18.47262526784499 76-77 18.815259966104676 31.562477674404395 30.77061708922329 18.85164527026764 78-79 19.647738149737393 31.481588049584065 30.51863772745454 18.352036073224003 80-81 19.01501542624415 31.563333188074015 31.10397161937521 18.317679766306625 82-83 19.953711853120204 31.983908547681004 29.66779628282469 18.394583316374106 84-85 19.743802501831016 30.78571360577514 30.35191210558582 19.118571786808022 86-87 19.68949057558504 31.95272553541036 29.89938431497883 18.458399574025773 88-89 18.910314594348772 31.597255055775914 30.934595223851975 18.557835126023342 90-91 19.20213364826346 32.19840761553563 30.40062951623486 18.19882921996605 92-93 19.61097787921064 31.288295252108355 29.908440762238463 19.19228610644254 94-95 19.207099369550722 31.069165470794687 30.475500506749047 19.248234652905545 96-97 19.579395583761553 31.50349615694616 30.150411857090482 18.766696402201806 98-99 20.055025813056098 30.456924376146137 30.1321742612064 19.35587554959136 100-101 20.82501959894387 31.89047352778977 28.61303902297626 18.671467850290096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10789.0 1 17493.0 2 19452.0 3 16673.0 4 21344.0 5 28104.0 6 36090.0 7 45076.5 8 56101.5 9 68113.5 10 80664.5 11 94639.5 12 110538.5 13 126539.5 14 144324.0 15 165274.0 16 187865.0 17 217227.0 18 252632.0 19 291129.5 20 352123.5 21 444370.0 22 570049.0 23 672699.5 24 794732.5 25 985999.5 26 1273187.0 27 1675196.0 28 1994447.5 29 2138662.0 30 2404431.0 31 2912973.5 32 3477436.0 33 3908075.5 34 4187367.5 35 4659285.0 36 5018060.5 37 5441811.5 38 5334460.5 39 4248603.5 40 3667185.0 41 3379956.5 42 2889787.5 43 2589928.5 44 2408844.0 45 2229343.5 46 2017619.5 47 1844920.5 48 1722889.5 49 1632431.0 50 1463251.0 51 1223811.0 52 1076748.0 53 960554.5 54 844513.0 55 725228.5 56 612561.5 57 509250.5 58 405694.0 59 312096.5 60 235124.0 61 172871.5 62 124841.0 63 87059.5 64 60539.5 65 43520.0 66 32107.5 67 24468.5 68 18794.5 69 14308.5 70 14280.5 71 16860.0 72 16606.5 73 20916.0 74 22427.0 75 15899.5 76 13087.5 77 13805.0 78 12843.5 79 8790.5 80 5621.5 81 3398.5 82 2059.5 83 1255.5 84 877.0 85 741.5 86 635.0 87 499.5 88 362.5 89 252.5 90 195.0 91 129.0 92 75.5 93 49.5 94 31.0 95 16.5 96 8.5 97 5.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015201073754566446 2 0.0022254899365516 3 5.114762366946986E-4 4 3.3984754393714424E-4 5 7.615312857454402E-5 6 1.4775980171180186E-4 7 1.0229524733893974E-4 8 8.94515107286062E-4 9 0.0010706902554809027 10-11 2.4039383124650838E-4 12-13 2.557381183473493E-4 14-15 4.8817565257860686E-4 16-17 0.001024089087248719 18-19 4.4157448434642324E-4 20-21 8.291598103750726E-4 22-23 0.0036917218150764033 24-25 3.216617221879994E-4 26-27 2.1254679169313036E-4 28-29 7.865367906505146E-4 30-31 1.6765054424992901E-4 32-33 3.307546330625718E-4 34-35 1.8299483135076998E-4 36-37 3.307546330625718E-4 38-39 2.8869992026767436E-4 40-41 8.149521371335532E-4 42-43 0.0016691174524137002 44-45 3.148420390320701E-4 46-47 0.004053165022340656 48-49 0.005734785227206894 50-51 0.0033200490830782555 52-53 7.387990085590093E-5 54-55 1.511696432897665E-4 56-57 3.2961801920325026E-4 58-59 4.4100617741676246E-4 60-61 1.2673244531435313E-4 62-63 2.0515880160754026E-4 64-65 2.455085936134554E-4 66-67 8.922418795674188E-5 68-69 4.489624744320133E-5 70-71 2.7392394009649417E-4 72-73 6.706021769997161E-5 74-75 1.9095112836602085E-4 76-77 5.8535613755059966E-5 78-79 2.7278732623717265E-5 80-81 4.660116823218366E-5 82-83 4.546455437286211E-6 84-85 7.331159392624016E-5 86-87 7.461869986445993E-4 88-89 4.7169475161844436E-5 90-91 5.967222761438151E-5 92-93 1.7560684126517989E-4 94-95 2.2163970256770278E-4 96-97 6.762852462963239E-4 98-99 9.405479685885848E-4 100-101 0.0378333289213772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8.7980627E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.7663419032899 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8894856292206 19.462135137486015 2 6.657950318391588 3.1647024728402458 3 2.8120152329684633 2.0049394639196367 4 1.5938534578530266 1.5152026489230352 5 1.0644328156189984 1.2648837114541325 6 0.7584447806242489 1.081527478264896 7 0.5836448646819264 0.9709772382891021 8 0.472998180740922 0.8993149186498289 9 0.3918700663992498 0.8381986181738537 >10 2.9967960046162396 14.505378137411057 >50 0.403191867538914 6.716016854823764 >100 0.31822224605675326 14.95639812405388 >500 0.032016372292567256 5.239654448625367 >1k 0.020849086093873308 9.546046184159893 >5k 0.002418761864908622 3.974885180791818 >10k+ 0.0018103150373139439 13.859739382133471 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 639826 0.7272350991542718 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 357068 0.4058484375202282 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 331270 0.37652607317745074 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 319897 0.36359936375538676 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 243812 0.2771200982689064 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 230870 0.2624100417015669 Illumina Single End PCR Primer 1 (100% over 50bp) CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT 197106 0.22403341135543398 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 178546 0.20293785812642595 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 139946 0.15906456315661402 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 138151 0.15702434127913184 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 103946 0.11814646422103811 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 97775 0.11113242009516483 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 97614 0.11094942526381404 No Hit CTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATT 96392 0.10956048312772311 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 91769 0.10430591725607957 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.38701474587127005 0.0 0.0 4.54645543728621E-6 0.0 2 0.38843892303699995 2.7278732623717265E-5 0.0 1.250275245253708E-5 0.0 3 0.388499163571544 2.7278732623717265E-5 0.0 1.4775980171180186E-5 0.0 4 0.40015627531274583 4.0918098935575895E-5 0.0 1.818582174914484E-5 0.0 5 0.40040292052021864 4.0918098935575895E-5 0.0 1.818582174914484E-5 0.0 6 0.40069389366820496 4.205471279489745E-5 0.0 1.9322435608466394E-5 0.0 7 0.40122696556822673 4.3191326654219E-5 0.0 2.3868891045752606E-5 0.0 8 0.40431855526558136 4.3191326654219E-5 0.0 2.7278732623717265E-5 0.0 9 0.40462316777987956 6.592360384065006E-5 0.0 3.0688574201681925E-5 1.1366138593215526E-6 10-11 0.4066236081722855 7.274328699657937E-5 0.0 3.2393494990664254E-5 1.1366138593215526E-6 12-13 0.4085047041094626 8.695096023809878E-5 0.0 3.864487121693279E-5 1.1366138593215526E-6 14-15 0.4104079640168966 9.206572260504577E-5 0.0 4.375963358387978E-5 1.1366138593215526E-6 16-17 0.4137831388721519 1.0513678198724363E-4 0.0 5.455746524743453E-5 1.1366138593215526E-6 18-19 0.4173293741132352 1.6196747495332125E-4 0.0 7.047005927793626E-5 1.1366138593215526E-6 20-21 0.4199691598015095 1.682188511795898E-4 0.0 8.41094255897949E-5 2.273227718643105E-6 22-23 0.4228243338161252 1.6878715810925057E-4 5.683069296607763E-7 8.808757409742033E-5 2.273227718643105E-6 24-25 0.4253265892274216 1.7390192047619756E-4 1.1366138593215526E-6 1.0002201962029664E-4 2.273227718643105E-6 26-27 0.4277794019358375 1.7674345512450144E-4 2.273227718643105E-6 1.2445921759571003E-4 2.273227718643105E-6 28-29 0.4307130023067465 1.7958498977280532E-4 2.273227718643105E-6 1.3809858390756866E-4 2.273227718643105E-6 30-31 0.4334817936680537 1.846997521397523E-4 2.273227718643105E-6 1.488964155711234E-4 2.273227718643105E-6 32-33 0.4359459725150629 1.898145145066993E-4 2.273227718643105E-6 1.5230625714908805E-4 2.273227718643105E-6 34-35 0.4385079001539737 1.949292768736463E-4 2.273227718643105E-6 1.5685271258637426E-4 2.273227718643105E-6 36-37 0.4407993136943659 2.0061234617025405E-4 2.273227718643105E-6 1.824265244211092E-4 2.273227718643105E-6 38-39 0.44353798478840123 2.0743202932618337E-4 2.273227718643105E-6 1.8867790064737775E-4 2.273227718643105E-6 40-41 0.44635962869416695 2.26186158004989E-4 2.273227718643105E-6 1.9777081152195017E-4 2.273227718643105E-6 42-43 0.44915456217423866 2.3016430651261443E-4 2.273227718643105E-6 2.1595663327109502E-4 2.273227718643105E-6 44-45 0.452238195574578 2.3414245502023987E-4 2.273227718643105E-6 2.2561785107532822E-4 2.273227718643105E-6 46-47 0.4561254149734577 2.3471076194990064E-4 2.273227718643105E-6 2.4096213817616916E-4 2.273227718643105E-6 48-49 0.45921984620546064 2.381206035278653E-4 2.273227718643105E-6 2.4891843519142006E-4 2.273227718643105E-6 50-51 0.4623858841106009 2.3868891045752605E-4 2.273227718643105E-6 2.580113460659925E-4 2.273227718643105E-6 52-53 0.4659821303614943 2.4380367282447307E-4 2.273227718643105E-6 2.665359500109041E-4 2.273227718643105E-6 54-55 0.4697198850378732 2.449402866837946E-4 2.273227718643105E-6 2.8017531632276276E-4 2.273227718643105E-6 56-57 0.47358835030807406 2.500550490507416E-4 2.273227718643105E-6 2.8869992026767436E-4 2.273227718643105E-6 58-59 0.477679591894702 2.523282767693847E-4 2.273227718643105E-6 2.9665621728292523E-4 2.273227718643105E-6 60-61 0.48202941313432557 2.568747322066709E-4 2.273227718643105E-6 3.0290759350919377E-4 2.273227718643105E-6 62-63 0.48698504956096755 2.6824087079988646E-4 2.273227718643105E-6 3.114321974541054E-4 2.273227718643105E-6 64-65 0.4920486643042451 2.7108240544819034E-4 2.273227718643105E-6 3.267764845549464E-4 2.273227718643105E-6 66-67 0.4979192748876409 2.875633064083528E-4 2.273227718643105E-6 3.45530613233752E-4 2.273227718643105E-6 68-69 0.5046662147565737 3.0063436579055066E-4 2.273227718643105E-6 3.5689675182696754E-4 2.273227718643105E-6 70-71 0.5122980085149882 3.1086389052444464E-4 2.8415346483038817E-6 3.8303887059136326E-4 2.8415346483038817E-6 72-73 0.5205935847672465 3.1256881131342697E-4 3.409841577964658E-6 4.012246923405081E-4 3.409841577964658E-6 74-75 0.5300803323440739 3.1313711824308775E-4 3.409841577964658E-6 4.427110982057448E-4 4.54645543728621E-6 76-77 0.540688349493122 3.137054251727485E-4 3.409841577964658E-6 5.268205237955397E-4 4.54645543728621E-6 78-79 0.5531666647476836 3.193884944693563E-4 3.409841577964658E-6 5.597823257158647E-4 4.54645543728621E-6 80-81 0.5671703158014547 3.210934152583386E-4 3.409841577964658E-6 6.336622265717656E-4 4.54645543728621E-6 82-83 0.5836478069200393 3.262081776252856E-4 3.409841577964658E-6 7.007224442717372E-4 4.54645543728621E-6 84-85 0.6039176101802501 3.2734479148460716E-4 3.409841577964658E-6 7.319793254030799E-4 4.54645543728621E-6 86-87 0.628374130591272 3.3757431621850114E-4 3.409841577964658E-6 7.461869986445993E-4 4.54645543728621E-6 88-89 0.6569201876681329 3.3984754393714424E-4 3.409841577964658E-6 7.609629788157795E-4 4.54645543728621E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 56210 0.0 67.121605 1 GCGTGTT 69035 0.0 53.92996 2 AGAGCGT 70770 0.0 47.117058 9 AAGAGCG 76755 0.0 44.439457 8 ATCGGAA 82360 0.0 42.63744 3 GATCGGA 83555 0.0 42.028362 2 GTTTTTT 1221185 0.0 41.737637 1 AGATCGG 84755 0.0 41.242073 1 CCTTTAG 101335 0.0 41.108192 5 CTCGCAT 14900 0.0 40.810295 1 CGGAAGA 85250 0.0 39.87165 5 TCGGAAG 87245 0.0 39.613087 4 CGCCGTA 45785 0.0 38.408543 46-47 CCGAAAC 182715 0.0 38.352463 1 CCGTATC 46060 0.0 38.312897 48-49 GTCGCCG 47080 0.0 37.443024 44-45 CGTGTTT 102510 0.0 36.573174 3 TTAGGCA 113805 0.0 36.265938 8 TAACGAG 112135 0.0 35.42956 94-95 CAAAACG 47340 0.0 35.11802 9 >>END_MODULE