FastQCFastQC Report
Fri 27 May 2016
SRR1233031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87980627
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA6106950.6941244008183756No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT3107170.35316524852681486No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA2990500.33990437463011036No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT2766030.3143908033299195No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2650530.3012629132547555TruSeq Adapter, Index 12 (100% over 49bp)
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC2494410.28351809768302744No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1931050.21948581930428843No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT1897890.21571680774677815No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1322000.15026035220230927No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC1085920.12342717221144606No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT936940.10649389893527357No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT906180.10299767470400047No Hit
CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG899060.10218840563616352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT577750.068.343191
GCGTGTT719650.054.1593972
AGATCGG794700.043.717251
ATCGGAA836250.041.5451553
GATCGGA854300.040.840562
GTTTTTT11974050.040.6770551
CCGAAAC1668750.040.2514721
CGGAAGA887550.039.9885255
TCGGAAG893000.039.163064
CTCGCAT157300.037.767651
CCTTTAG948800.037.55225
CGTGTTT1060600.036.9771273
TATGCCG592950.036.3332246-47
TGCCGTC592500.035.64298248-49
CGAAACC1907500.035.0378342
CCGTCTT609200.034.5840850-51
TAACGGC94550.034.557979
CCAAACG1984850.034.0772257
TAACGAG1007900.033.95404494-95
CGTCTGA661900.033.72499516-17
Produced by FastQC (version 0.11.5)