##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233031_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87980627 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.936006002776043 31.0 30.0 34.0 26.0 34.0 2 30.362878125430953 31.0 30.0 34.0 27.0 34.0 3 29.81968952096693 31.0 30.0 34.0 26.0 34.0 4 33.20588044911296 35.0 33.0 37.0 27.0 37.0 5 33.03489063563959 35.0 33.0 37.0 26.0 37.0 6 33.10153885354784 35.0 33.0 37.0 27.0 37.0 7 33.3052475177291 35.0 35.0 37.0 28.0 37.0 8 33.18407508052881 35.0 35.0 37.0 27.0 37.0 9 34.440623763683796 38.0 35.0 39.0 27.0 39.0 10-11 34.08427579744345 37.5 34.0 39.0 26.0 39.0 12-13 33.912943408552884 37.0 33.0 39.0 25.0 39.0 14-15 34.756484168952326 38.0 33.5 40.0 25.0 41.0 16-17 34.69345749831949 38.0 34.0 40.0 24.5 41.0 18-19 34.417329891272544 38.5 34.0 40.0 22.0 41.0 20-21 34.1329827701728 38.0 33.0 40.0 18.5 41.0 22-23 33.85781162368848 38.0 33.0 40.0 18.0 41.0 24-25 33.596388583363925 38.0 33.0 40.0 13.5 41.0 26-27 33.221114234614404 38.0 33.0 40.0 9.5 41.0 28-29 32.89555481344773 38.0 32.0 40.0 5.0 41.0 30-31 32.65314852780033 38.0 32.0 40.0 2.0 41.0 32-33 32.402905028171716 38.0 31.5 40.0 2.0 41.0 34-35 32.15312420426374 38.0 31.0 40.0 2.0 41.0 36-37 31.911403046718455 38.0 30.5 40.0 2.0 41.0 38-39 31.733997121889118 38.0 30.0 40.0 2.0 41.0 40-41 31.513096729806207 38.0 30.0 40.0 2.0 41.0 42-43 31.297152786828853 37.0 30.0 40.0 2.0 41.0 44-45 31.090675479046084 37.0 30.0 40.0 2.0 41.0 46-47 30.803233761905332 37.0 29.5 40.0 2.0 41.0 48-49 30.648994505347183 37.0 29.0 40.0 2.0 41.0 50-51 30.533490441026295 37.0 29.0 40.0 2.0 41.0 52-53 30.22803845783004 36.5 28.0 39.5 2.0 40.5 54-55 29.884945489192752 36.0 27.5 39.0 2.0 40.0 56-57 29.681164172653602 36.0 27.0 39.0 2.0 40.0 58-59 29.35132147898878 35.5 26.5 39.0 2.0 40.0 60-61 28.946472363739804 35.0 26.0 39.0 2.0 40.0 62-63 28.5265311703223 34.5 25.0 38.5 2.0 40.0 64-65 28.0258820956118 34.0 23.0 38.0 2.0 40.0 66-67 27.5931276438846 34.0 22.0 38.0 2.0 40.0 68-69 27.03015154120236 33.5 21.5 37.5 2.0 39.5 70-71 26.441093503459577 32.5 18.5 37.0 2.0 39.0 72-73 25.94073077019558 32.0 17.5 36.0 2.0 39.0 74-75 25.42193481412675 31.5 13.0 36.0 2.0 39.0 76-77 24.766306513137263 31.0 12.5 35.0 2.0 38.0 78-79 24.92877768420541 31.0 8.0 35.0 2.0 38.0 80-81 24.510327682706787 31.0 7.0 35.0 2.0 37.0 82-83 23.92850049818354 31.0 2.0 35.0 2.0 36.5 84-85 23.33936322140555 30.5 2.0 34.0 2.0 36.0 86-87 22.59298697655337 30.0 2.0 34.0 2.0 36.0 88-89 22.00959466906277 29.0 2.0 34.0 2.0 35.0 90-91 21.39852797934709 29.0 2.0 34.0 2.0 35.0 92-93 20.603357350476713 29.0 2.0 34.0 2.0 35.0 94-95 19.70966144058055 26.5 2.0 33.5 2.0 35.0 96-97 18.631614548507365 25.5 2.0 33.0 2.0 34.5 98-99 17.23381006366322 21.0 2.0 32.5 2.0 34.0 100-101 12.895114938201111 2.0 2.0 28.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5147431781213818 1101 2 0.47976903210050637 1101 3 1.0633356989231437 1101 4 0.9641888748579177 1101 5 0.7716257324136393 1101 6 0.6473055258738043 1101 7 0.6083441537009904 1101 8 0.6184296757798364 1101 9 0.6227190397126421 1101 10-11 0.6793846870400699 1101 12-13 0.7097207218752573 1101 14-15 0.7583300657028076 1101 16-17 0.7251226911827047 1101 18-19 0.7600290200863995 1101 20-21 0.789605898546661 1101 22-23 0.7813129862052861 1101 24-25 0.7579130794578361 1101 26-27 0.7596034681118269 1101 28-29 0.7535357047735047 1101 30-31 0.7782217386680443 1101 32-33 0.7350937443489514 1101 34-35 0.7252039083006281 1101 36-37 0.8312376236653378 1101 38-39 0.816600238562966 1101 40-41 0.8137308039821818 1101 42-43 0.8373982523036396 1101 44-45 0.8164611965055002 1101 46-47 0.7721998225989779 1101 48-49 0.9193387194650882 1101 50-51 1.0399049318659515 1101 52-53 1.0290855699125778 1101 54-55 0.9983214572369654 1101 56-57 1.1329496064089852 1101 58-59 1.208282232142217 1101 60-61 1.3288535372966024 1101 62-63 1.3978417385356678 1101 64-65 1.39599466732885 1101 66-67 1.3600584002486542 1101 68-69 1.502168400936494 1101 70-71 1.51927074929214 1101 72-73 1.6381801741193094 1101 74-75 1.5969637809539599 1101 76-77 1.5252372851355176 1101 78-79 1.634971734489561 1101 80-81 1.8106280728449278 1101 82-83 1.9631004876684734 1101 84-85 1.966101654615791 1101 86-87 2.0363028658647124 1101 88-89 2.127196712245908 1101 90-91 2.2544862505916967 1101 92-93 2.4537683111653905 1101 94-95 2.471710055913359 1101 96-97 2.4645758185197906 1101 98-99 2.604667350818495 1101 100-101 2.8914887577254493 1102 1 0.44491673778226115 1102 2 0.40391518529959924 1102 3 0.9543389470132304 1102 4 0.9123423190109818 1102 5 0.6887534133828552 1102 6 0.600252620978381 1102 7 0.5128390654910646 1102 8 0.5581510632967195 1102 9 0.5088467694071142 1102 10-11 0.5482125459677718 1102 12-13 0.6267984300040013 1102 14-15 0.6919766455744565 1102 16-17 0.612463529311988 1102 18-19 0.5734079257668228 1102 20-21 0.5842486830596556 1102 22-23 0.6372608925320691 1102 24-25 0.6661563446027543 1102 26-27 0.6958210640593805 1102 28-29 0.6516026267356665 1102 30-31 0.6534461477400626 1102 32-33 0.6018532944814154 1102 34-35 0.6590130583762104 1102 36-37 0.751833663904371 1102 38-39 0.7579071304383227 1102 40-41 0.7995100770868859 1102 42-43 0.7432531325696878 1102 44-45 0.765008341344803 1102 46-47 0.7914284121201121 1102 48-49 0.8488052444542049 1102 50-51 0.8713915150341656 1102 52-53 0.9465793614604081 1102 54-55 0.9584824584375617 1102 56-57 1.1009645614007297 1102 58-59 1.2072346428547895 1102 60-61 1.2618945803092672 1102 62-63 1.2811226025723634 1102 64-65 1.2503882713225352 1102 66-67 1.4035504677370803 1102 68-69 1.5781854505169797 1102 70-71 1.541511836980252 1102 72-73 1.637652745783999 1102 74-75 1.6457388449841268 1102 76-77 1.5712330450389516 1102 78-79 1.6848442134542232 1102 80-81 1.8275383947713486 1102 82-83 1.9210966660166982 1102 84-85 1.947475836790037 1102 86-87 2.0804107474208067 1102 88-89 2.2774925774528576 1102 90-91 2.3992277123158274 1102 92-93 2.5758396138923665 1102 94-95 2.488399560940689 1102 96-97 2.6001017496991423 1102 98-99 2.7817647295056958 1102 100-101 3.045437857749077 1103 1 0.462290911056936 1103 2 0.4174286290662863 1103 3 1.004881847209802 1103 4 0.9105032556969945 1103 5 0.7185743975980472 1103 6 0.6037716353379281 1103 7 0.5478608397558844 1103 8 0.545971500855174 1103 9 0.5137371976310234 1103 10-11 0.5831350808209663 1103 12-13 0.6126910355355975 1103 14-15 0.6611128709890295 1103 16-17 0.6298581580474192 1103 18-19 0.6104490017896396 1103 20-21 0.547060943199078 1103 22-23 0.6281409059785616 1103 24-25 0.5970178530399508 1103 26-27 0.6342768722984218 1103 28-29 0.6130411475367694 1103 30-31 0.6463706447109487 1103 32-33 0.6624827929360535 1103 34-35 0.7198781225406705 1103 36-37 0.767265729782828 1103 38-39 0.7903741011386991 1103 40-41 0.7714868237780834 1103 42-43 0.8001452884504765 1103 44-45 0.8288778116905533 1103 46-47 0.8433693579614285 1103 48-49 0.956022220223943 1103 50-51 0.9558580776727119 1103 52-53 0.9656905343560176 1103 54-55 0.9548175197219209 1103 56-57 1.1074604532759835 1103 58-59 1.188926885710611 1103 60-61 1.278140811593694 1103 62-63 1.3193477629822574 1103 64-65 1.355577523525639 1103 66-67 1.3765509368258293 1103 68-69 1.4679628274382708 1103 70-71 1.5031073425745376 1103 72-73 1.5653981482212522 1103 74-75 1.6277318903365092 1103 76-77 1.5182324650351404 1103 78-79 1.6189441951632126 1103 80-81 1.8011704344351038 1103 82-83 1.9519684193050288 1103 84-85 2.0123889418974628 1103 86-87 2.1475794909545343 1103 88-89 2.3368091890763445 1103 90-91 2.4317693024419746 1103 92-93 2.6422943475646754 1103 94-95 2.742948555479977 1103 96-97 2.8920108079019577 1103 98-99 3.0868331547052392 1103 100-101 3.200596700813511 1104 1 0.30796190733896367 1104 2 0.29252900769573387 1104 3 0.8318327314414162 1104 4 0.7773923840283672 1104 5 0.4396505131932855 1104 6 0.41631259728732317 1104 7 0.3445731182205378 1104 8 0.31067951098742697 1104 9 0.3525091829863243 1104 10-11 0.35818070298701343 1104 12-13 0.37316783847903423 1104 14-15 0.40323956629495683 1104 16-17 0.374806851307973 1104 18-19 0.3514140836555413 1104 20-21 0.34972729846966644 1104 22-23 0.41244705001883375 1104 24-25 0.4041624874658183 1104 26-27 0.4275409296778463 1104 28-29 0.42621969664696024 1104 30-31 0.4716904138727642 1104 32-33 0.46998052792665845 1104 34-35 0.5031419177180467 1104 36-37 0.5126999804709769 1104 38-39 0.5276445172030684 1104 40-41 0.47918544051753287 1104 42-43 0.5371698332914256 1104 44-45 0.5785208965955917 1104 46-47 0.5748004955512442 1104 48-49 0.662726654663274 1104 50-51 0.7139418807851214 1104 52-53 0.5967868829868692 1104 54-55 0.7194916582664064 1104 56-57 0.8890365379761462 1104 58-59 0.9296478691287469 1104 60-61 1.0043961072727257 1104 62-63 1.0492142743873138 1104 64-65 1.0946901920888124 1104 66-67 1.178436205493398 1104 68-69 1.2963781106733663 1104 70-71 1.2908700826562232 1104 72-73 1.3624256283506035 1104 74-75 1.4483398763776236 1104 76-77 1.3331367031355086 1104 78-79 1.453777555961274 1104 80-81 1.5852628315436803 1104 82-83 1.718920032471356 1104 84-85 1.7828133485711746 1104 86-87 1.963009576629517 1104 88-89 2.128643969299606 1104 90-91 2.266300863888791 1104 92-93 2.4771611246397676 1104 94-95 2.539528306387595 1104 96-97 2.7439381124894027 1104 98-99 2.9533284590136866 1104 100-101 2.9987729733355923 1105 1 0.11220576253733938 1105 2 0.04463349651338433 1105 3 0.6079557990070725 1105 4 0.5476140981078359 1105 5 0.2449014620759442 1105 6 0.1008697094751696 1105 7 0.09398645269639161 1105 8 0.047819726633427706 1105 9 0.023206172701591754 1105 10-11 0.039969111644317934 1105 12-13 0.015561724128744459 1105 14-15 -0.006013904784722968 1105 16-17 0.043830988959236095 1105 18-19 -0.01140532134348149 1105 20-21 0.03279444337849924 1105 22-23 0.05931554232889624 1105 24-25 0.1044307232881252 1105 26-27 0.11176795259105887 1105 28-29 0.10885776776932232 1105 30-31 0.07901168364514177 1105 32-33 0.1774822232156339 1105 34-35 0.15167843744586662 1105 36-37 0.23016118528578744 1105 38-39 0.2595971581165877 1105 40-41 0.260987592057333 1105 42-43 0.25668412676418484 1105 44-45 0.24156820859682426 1105 46-47 0.3321951921275357 1105 48-49 0.3943103130709744 1105 50-51 0.3810597856030533 1105 52-53 0.420399661008247 1105 54-55 0.4193404484536458 1105 56-57 0.4930867952276756 1105 58-59 0.6147286120917705 1105 60-61 0.6880776546905736 1105 62-63 0.7396206004916017 1105 64-65 0.7155611211136161 1105 66-67 0.7977902093097597 1105 68-69 0.890625042347061 1105 70-71 0.9531156374569782 1105 72-73 1.074894534718151 1105 74-75 1.1689705455031323 1105 76-77 1.0256681931482774 1105 78-79 1.0323035970551366 1105 80-81 1.1546746952809883 1105 82-83 1.3342237808718416 1105 84-85 1.3270087850279317 1105 86-87 1.5196174768586204 1105 88-89 1.7377140106078208 1105 90-91 1.874555617155206 1105 92-93 2.089926402407716 1105 94-95 2.2828892404941925 1105 96-97 2.390827214631301 1105 98-99 2.607075352803328 1105 100-101 2.6544764335654367 1106 1 -0.7751975012599246 1106 2 -0.724828852996378 1106 3 -3.4039827161654586 1106 4 -1.5631512162172925 1106 5 -1.0589245027435936 1106 6 -0.9401860877322292 1106 7 -0.8101416213766655 1106 8 -0.7838482589901261 1106 9 -0.8525911526022227 1106 10-11 -0.8785876828552404 1106 12-13 -0.901781969093534 1106 14-15 -0.9112954569271352 1106 16-17 -0.912899096250996 1106 18-19 -0.9233897592485718 1106 20-21 -0.8969972950913601 1106 22-23 -0.940697901901423 1106 24-25 -0.9386972985565123 1106 26-27 -0.9087472572785416 1106 28-29 -0.8782929979556471 1106 30-31 -0.8680259118829667 1106 32-33 -0.8510207560418479 1106 34-35 -0.9037655134820142 1106 36-37 -0.8396259965958421 1106 38-39 -0.8640745593888681 1106 40-41 -0.8501330886725853 1106 42-43 -0.8359836854333373 1106 44-45 -0.7705491285574126 1106 46-47 -0.7817878270237273 1106 48-49 -0.8100365863604893 1106 50-51 -0.6895683215346402 1106 52-53 -0.7494543322190239 1106 54-55 -0.7095114523489201 1106 56-57 -0.6582093120475889 1106 58-59 -0.5466089606572169 1106 60-61 -0.48878541075582405 1106 62-63 -0.4404373166525275 1106 64-65 -0.40728871325048743 1106 66-67 -0.43864763154458686 1106 68-69 -0.2998159862893104 1106 70-71 -0.2613081115619842 1106 72-73 -0.19352232256317947 1106 74-75 -0.08647525816730806 1106 76-77 -0.10364046018101192 1106 78-79 -0.09443390999265944 1106 80-81 0.04528084711984803 1106 82-83 0.26053510736388574 1106 84-85 0.266286895618439 1106 86-87 0.388430518354852 1106 88-89 0.5639541553138407 1106 90-91 0.6523888093368662 1106 92-93 0.8869764939401357 1106 94-95 1.054345885796966 1106 96-97 1.071062864946473 1106 98-99 1.2391420674928852 1106 100-101 1.4798724711053222 1108 1 -3.02339324194428 1108 2 -2.5588432288054044 1108 3 -5.886398107727075 1108 4 -6.936141117320432 1108 5 -4.729415174654797 1108 6 -3.8678725786194548 1108 7 -3.6187076196200785 1108 8 -3.3162513313061552 1108 9 -3.496189861875944 1108 10-11 -3.7784088663863287 1108 12-13 -3.6864856360081966 1108 14-15 -3.8945684376331577 1108 16-17 -3.7059406622278104 1108 18-19 -3.7104301069767978 1108 20-21 -3.6876595679351674 1108 22-23 -3.819230793383717 1108 24-25 -3.8852208260837884 1108 26-27 -3.735405190431237 1108 28-29 -3.673647203368496 1108 30-31 -3.6462676915619667 1108 32-33 -3.786258236723768 1108 34-35 -3.8362256257311635 1108 36-37 -3.8154917142139055 1108 38-39 -3.808065251094412 1108 40-41 -3.726328624736176 1108 42-43 -3.785613276966121 1108 44-45 -3.906673752930608 1108 46-47 -3.888965341795931 1108 48-49 -3.8901954577429407 1108 50-51 -3.9205459660350606 1108 52-53 -3.819846856453232 1108 54-55 -3.7979534470708884 1108 56-57 -3.7697175898742685 1108 58-59 -3.679072668104279 1108 60-61 -3.6537546573306514 1108 62-63 -3.570094432758804 1108 64-65 -3.505347479330993 1108 66-67 -3.549915440500481 1108 68-69 -3.4414778219766617 1108 70-71 -3.327265501424119 1108 72-73 -3.2788994539707623 1108 74-75 -3.1386609054106067 1108 76-77 -3.1481648643936673 1108 78-79 -3.1812541179014424 1108 80-81 -3.3009861359246386 1108 82-83 -3.196472836994044 1108 84-85 -2.9660094649976045 1108 86-87 -2.796058601141656 1108 88-89 -2.5812781717758533 1108 90-91 -2.433965397697609 1108 92-93 -2.26824298865478 1108 94-95 -2.2138273678481646 1108 96-97 -2.1101640289723775 1108 98-99 -1.8868677262504328 1108 100-101 -1.4525491321520665 1201 1 0.6573721046648089 1201 2 0.6144436247195131 1201 3 1.1460776293235853 1201 4 1.1124007345462061 1201 5 0.8865878278313275 1201 6 0.7404577388860503 1201 7 0.7300221318630591 1201 8 0.714911770869314 1201 9 0.7340997057963392 1201 10-11 0.8341864909268679 1201 12-13 0.7486497493415101 1201 14-15 0.8583075508930946 1201 16-17 0.9332987717655143 1201 18-19 0.9346937655725469 1201 20-21 0.9393634709577015 1201 22-23 0.9241249951728108 1201 24-25 0.9220189823982352 1201 26-27 0.8908673683976573 1201 28-29 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1.6620338136784163 2304 100-101 1.049366937473625 2305 1 -0.25188417935088836 2305 2 -0.24638829269835227 2305 3 -1.014690876997669 2305 4 -0.2068470958781532 2305 5 -0.3123921750569991 2305 6 -0.2864247468187031 2305 7 -0.2576645522669665 2305 8 -0.2570662407139679 2305 9 -0.2655718259162114 2305 10-11 -0.2814054536981416 2305 12-13 -0.3088564583188145 2305 14-15 -0.3824505763156836 2305 16-17 -0.434926242706247 2305 18-19 -0.4486848547360225 2305 20-21 -0.3877747537910139 2305 22-23 -0.38550581020697194 2305 24-25 -0.31114247176453347 2305 26-27 -0.2902452271783389 2305 28-29 -0.2777480215695576 2305 30-31 -0.2782707434378935 2305 32-33 -0.2791576734525023 2305 34-35 -0.37110887864635345 2305 36-37 -0.35474259256059426 2305 38-39 -0.3223172131609253 2305 40-41 -0.2512060871758557 2305 42-43 -0.19568014330940642 2305 44-45 -0.19069139358622422 2305 46-47 -0.15297409670838036 2305 48-49 -0.17923805872143816 2305 50-51 -0.13566465670205474 2305 52-53 -0.09908063999245087 2305 54-55 -0.03305243339006836 2305 56-57 -0.06009571336807085 2305 58-59 -0.017606242286557716 2305 60-61 -0.0068809400365381634 2305 62-63 -0.08237928730043365 2305 64-65 0.03713613975932617 2305 66-67 0.1496920749426529 2305 68-69 0.2055135360030853 2305 70-71 0.12462147129744139 2305 72-73 0.18127585375139788 2305 74-75 0.18819584235344067 2305 76-77 0.19051449336037507 2305 78-79 0.428824901193142 2305 80-81 0.3102167494806807 2305 82-83 0.27693112575806467 2305 84-85 0.4764324032063385 2305 86-87 0.4585465859355615 2305 88-89 0.43177908122998687 2305 90-91 0.6268378234906855 2305 92-93 0.6783182745900689 2305 94-95 0.6484038087843693 2305 96-97 0.7581161059510535 2305 98-99 0.9281542185071139 2305 100-101 0.5456613437063051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2845238.0 3 990520.0 4 583222.0 5 539869.0 6 842472.0 7 778914.0 8 751136.0 9 989004.0 10 1236909.0 11 1116751.0 12 908370.0 13 765537.0 14 715369.0 15 716861.0 16 739448.0 17 774269.0 18 816114.0 19 860911.0 20 919132.0 21 987378.0 22 1078989.0 23 1194188.0 24 1340225.0 25 1525112.0 26 1746100.0 27 1994870.0 28 2314432.0 29 2707455.0 30 3186653.0 31 3773113.0 32 4512838.0 33 5470382.0 34 6692063.0 35 8238118.0 36 9889195.0 37 9573200.0 38 3718569.0 39 147699.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.53542357239368 17.04259838003774 11.563937390617115 46.85804065695147 2 13.214789089399614 18.01786955673697 55.665210537778044 13.102130816085374 3 16.569687551783417 24.075125084071065 39.51096415805266 19.84422320609286 4 18.951402085578696 31.78436698578329 32.375387105007306 16.888843823630705 5 18.462391705094685 33.960088615482874 32.84504220588119 14.732477473541246 6 14.842976155395293 36.94646636035529 33.02519154880669 15.185365935442732 7 12.655476292364954 18.43603923205698 51.733568850766 17.174915624812066 8 13.052783914876587 21.617584426087028 44.32235721338839 21.007274445647994 9 15.35368810957736 23.334226608573005 41.838572128102676 19.473513153746964 10-11 19.248619668856943 34.98242631000939 30.459447051311876 15.309506969821795 12-13 17.707970617696592 26.099035448787888 38.510548985432784 17.682444948082733 14-15 18.427436160289467 27.824244728147562 37.18642681514151 16.56189229642146 16-17 18.57610587014436 27.406530385552102 36.12996241148859 17.887401332814946 18-19 19.33373975056838 28.684940422731707 34.446171568344475 17.535148258355438 20-21 19.0787230421135 28.015548909701245 34.53737268865264 18.368355359532618 22-23 18.93211582740466 31.016432078455548 33.65048293817024 16.400969155969552 24-25 17.951222040472818 30.77435698268743 33.150010569437455 18.1244104074023 26-27 18.2545297123731 30.40990997448802 32.81987613469835 18.515684178440534 28-29 18.182605491224532 31.20877730669332 32.24457588862033 18.36404131346182 30-31 17.76662660832234 30.286022378708218 33.40286522503825 18.5444857879312 32-33 18.00264839484304 30.608774308610975 33.00948922024596 18.379088076300025 34-35 18.459878808953768 31.50331779678786 30.9018488364241 19.13495455783427 36-37 19.203564847792432 31.04891174019744 31.83616347597718 17.91135993603295 38-39 18.99244531813485 30.44828960730626 32.06750632162649 18.491758752932398 40-41 19.25363472980811 31.125986042063523 31.04261098538294 18.577768242745428 42-43 18.840365889391265 31.057242687550584 31.726105850656154 18.376285572401997 44-45 19.299721960602195 30.127549995890924 31.658425609749667 18.91430243375721 46-47 19.61425210895116 29.473377919081912 31.987391256186214 18.924978715780714 48-49 19.04477684891112 30.07363870421495 31.73293762259312 19.148646824280807 50-51 18.242183027870176 30.290744647973955 32.99793268602984 18.469139638126027 52-53 19.841725254051344 30.135963310586998 31.705521170001855 18.3167902653598 54-55 19.632395291397895 30.305242313121177 31.151682813433084 18.910679582047845 56-57 19.693289483811757 30.405967317943638 31.00391363381868 18.89682956442592 58-59 19.01967963656834 31.157048009654027 30.68103083842545 19.142241515352183 60-61 19.49365805481389 31.086448685799688 30.21101157982612 19.208881679560303 62-63 19.61893173171501 31.61644466973124 31.001013801964028 17.76360979658972 64-65 20.175889184793007 30.962836132384542 30.51626502404835 18.345009658774096 66-67 19.509969966974325 32.16982536409005 29.767265169649924 18.5529394992857 68-69 19.640386117841253 31.83733554811153 30.30243202217358 18.219846311873635 70-71 19.92178234127924 31.742068503392606 29.853959136831836 18.48219001849632 72-73 18.882468427168003 31.620163401592183 30.85718212293224 18.640186048307573 74-75 18.89442263476139 29.998927679686794 32.598684765195905 18.507964920355914 76-77 18.747023018436 31.589506395047124 30.790544245622364 18.872926340894516 78-79 19.51137217224748 31.589735501832944 30.52988784655272 18.36900447936685 80-81 18.94769254553624 31.615034254663037 31.09731609521566 18.339957104585068 82-83 19.84070190032535 31.95087943924374 29.77061617638266 18.437802484048255 84-85 19.632376347098642 30.84070126500091 30.405034798978463 19.121887588921982 86-87 19.58983213846016 31.941695866028745 29.9018030331483 18.566668962362797 88-89 18.81488986006773 31.681129623273442 30.87894564802213 18.6250348686367 90-91 19.078106502149765 32.19900880650414 30.437265806381948 18.28561888496415 92-93 19.429070738205088 31.3544504776952 29.97616386662174 19.240314917477974 94-95 19.012750977217806 31.200350966761242 30.50968115772921 19.277216898291744 96-97 19.331208585448678 31.587340933081183 30.169912949262567 18.911537532207568 98-99 19.806107868801 30.631247358395957 30.18034217944765 19.382302593355394 100-101 19.936534664540382 32.44617925042499 28.60147710132037 19.015808983714262 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10618.0 1 15097.5 2 15790.0 3 13399.5 4 17364.0 5 23205.0 6 29592.0 7 37243.0 8 47125.5 9 58169.5 10 70633.0 11 84602.5 12 100644.5 13 117649.5 14 135711.0 15 157034.0 16 181610.5 17 211752.0 18 248113.0 19 289010.5 20 350961.0 21 441548.5 22 562527.0 23 670444.5 24 800969.0 25 997959.0 26 1286288.5 27 1688231.5 28 2009992.0 29 2158767.5 30 2430451.0 31 2910959.0 32 3463219.5 33 3921059.5 34 4215254.0 35 4659135.5 36 5025105.5 37 5448678.5 38 5321142.5 39 4269651.0 40 3668932.0 41 3373905.0 42 2903326.5 43 2592286.5 44 2411924.0 45 2235198.5 46 2025054.0 47 1849306.5 48 1725717.0 49 1630410.5 50 1454516.0 51 1215824.5 52 1067126.0 53 950393.0 54 835424.5 55 715878.5 56 604473.5 57 504194.5 58 402326.5 59 311072.5 60 235810.0 61 175414.5 62 128073.0 63 90704.5 64 64018.5 65 45807.0 66 33885.0 67 26146.5 68 20524.0 69 16018.5 70 15548.5 71 17777.0 72 17165.5 73 20786.0 74 22028.0 75 15911.0 76 13348.0 77 13138.5 78 12167.5 79 9313.5 80 6391.0 81 4077.5 82 2517.5 83 1524.5 84 946.0 85 703.5 86 623.5 87 490.5 88 340.0 89 228.5 90 180.5 91 146.0 92 96.5 93 48.5 94 24.5 95 9.0 96 3.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041041989846241946 2 0.0034859947065392018 3 0.0 4 0.06677265439356325 5 0.0016128550663772833 6 0.004207744507208388 7 0.002114101778338088 8 0.008482549232116748 9 0.02237197059302612 10-11 0.017415197552524832 12-13 0.016226867762604145 14-15 0.024930488390358937 16-17 0.020888689506611494 18-19 0.007019158888240248 20-21 0.009270790943556245 22-23 0.015187434388254586 24-25 0.02212418877169402 26-27 0.02538968038952484 28-29 0.03907451125575634 30-31 0.059819419109163656 32-33 0.033663660978456085 34-35 0.02658142002102349 36-37 0.02880520503678611 38-39 0.04169724773614082 40-41 0.043484573029923965 42-43 0.06423516395262789 44-45 0.053677157813389983 46-47 0.05401359551574917 48-49 0.051929045697753445 50-51 0.06052184647422437 52-53 0.058537318675848944 54-55 0.0593738664763096 56-57 0.041518231053297674 58-59 0.04749056857710278 60-61 0.03733435543713504 62-63 0.013377945124214675 64-65 0.025594839191132385 66-67 0.028551740146157405 68-69 0.030231087123305 70-71 0.029043325640313977 72-73 0.03474173922402258 74-75 0.035416887856459585 76-77 0.026660982991176 78-79 0.02017375938909824 80-81 0.01233453360135749 82-83 0.0014747564824697146 84-85 0.005501779386045976 86-87 0.013261442203634215 88-89 0.014734493765314948 90-91 0.0066798796512327644 92-93 0.016592289118376027 94-95 0.019028620925831775 96-97 0.016039894782745753 98-99 0.00698335555167162 100-101 0.23192435307377385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8.7980627E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.327058249094154 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36911046273275 18.981019794411434 2 6.910200165831758 3.22389283562515 3 2.794133450124223 1.9553674124037053 4 1.6397891582334143 1.5300582884137568 5 1.1227351883107264 1.3095054568016002 6 0.7848904971756792 1.0985511808066524 7 0.5969990622233121 0.9748362329396452 8 0.49263252560482196 0.9193334096145638 9 0.37778147495709896 0.7931277424577656 >10 3.096663263295435 14.708510493749024 >50 0.4226566694919047 6.897727650595066 >100 0.334417408278393 15.292605937874807 >500 0.03239045460687572 5.188437857000375 >1k 0.021322576558116917 9.578688074476045 >5k 0.0024572169692524373 3.977012171148659 >10k+ 0.001820425606480674 13.571325461682124 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 610695 0.6941244008183756 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 310717 0.35316524852681486 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 299050 0.33990437463011036 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 276603 0.3143908033299195 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG 265053 0.3012629132547555 TruSeq Adapter, Index 12 (100% over 49bp) CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 249441 0.28351809768302744 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 193105 0.21948581930428843 No Hit CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT 189789 0.21571680774677815 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 132200 0.15026035220230927 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 108592 0.12342717221144606 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 93694 0.10649389893527357 No Hit ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT 90618 0.10299767470400047 No Hit CTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAG 89906 0.10218840563616352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.393066078058298 1.1366138593215526E-6 0.0 6.819683155929316E-6 0.0 2 0.3945743646496177 1.9322435608466394E-5 0.0 9.09291087457242E-6 0.0 3 0.3947323539760634 1.9322435608466394E-5 0.0 1.0229524733893974E-5 0.0 4 0.398403616741672 2.841534648303882E-5 0.0 1.818582174914484E-5 0.0 5 0.39875824026578033 2.841534648303882E-5 0.0 1.818582174914484E-5 0.0 6 0.39924925745300727 2.841534648303882E-5 1.1366138593215526E-6 2.500550490507416E-5 0.0 7 0.39970390299673586 2.955196034236037E-5 1.1366138593215526E-6 3.0688574201681925E-5 0.0 8 0.4031057882776853 3.0688574201681925E-5 1.1366138593215526E-6 3.182518806100348E-5 0.0 9 0.4035024665145885 5.5694079106756084E-5 1.1366138593215526E-6 3.296180192032503E-5 0.0 10-11 0.4053773110755394 6.649191077031082E-5 1.1366138593215526E-6 3.637164349828968E-5 0.0 12-13 0.407694866734696 8.013127708216946E-5 1.1366138593215526E-6 3.978148507625434E-5 0.0 14-15 0.4098311324832909 9.831709883131431E-5 1.1366138593215526E-6 4.546455437286211E-5 0.0 16-17 0.41330576105123684 1.0684170277622596E-4 1.1366138593215526E-6 5.626238603641686E-5 0.0 18-19 0.4168150563418922 1.7390192047619756E-4 1.1366138593215526E-6 7.67214355042048E-5 0.0 20-21 0.41921047005041234 1.824265244211092E-4 1.1366138593215526E-6 9.888540576097508E-5 0.0 22-23 0.42155757766991137 1.846997521397523E-4 1.1366138593215526E-6 1.0741000970588673E-4 0.0 24-25 0.4234568594288377 1.9663419766262862E-4 1.1366138593215526E-6 1.3241551461096087E-4 0.0 26-27 0.42513222825747765 1.972025045922894E-4 1.7049207889823289E-6 1.6480900960162513E-4 5.683069296607763E-7 28-29 0.4270525373728014 1.9833911845161095E-4 2.273227718643105E-6 1.72765306616876E-4 1.1366138593215526E-6 30-31 0.42893704315155656 2.0345388081855793E-4 2.273227718643105E-6 1.8413144521009153E-4 1.1366138593215526E-6 32-33 0.4305987726138847 2.114101778338088E-4 2.273227718643105E-6 1.8583636599907386E-4 1.1366138593215526E-6 34-35 0.4323412016602246 2.2220800949736356E-4 2.273227718643105E-6 1.9265604915500318E-4 1.1366138593215526E-6 36-37 0.43390802386529936 2.3527906887956142E-4 2.273227718643105E-6 2.26186158004989E-4 1.1366138593215526E-6 38-39 0.43576581921836044 2.403938312465084E-4 2.273227718643105E-6 2.3641568273888297E-4 1.1366138593215526E-6 40-41 0.4376565763733418 2.602845737846356E-4 2.273227718643105E-6 2.506233559804024E-4 2.273227718643105E-6 42-43 0.43958484178568086 2.6824087079988646E-4 2.273227718643105E-6 2.807436232524235E-4 2.273227718643105E-6 44-45 0.4417733917718045 2.69377484659208E-4 2.273227718643105E-6 3.0290759350919377E-4 2.273227718643105E-6 46-47 0.44393466302530443 2.7051409851852956E-4 2.273227718643105E-6 3.387109300778227E-4 3.409841577964658E-6 48-49 0.4461880000014094 2.716507123778511E-4 2.273227718643105E-6 3.483721478820559E-4 3.409841577964658E-6 50-51 0.4485998946108897 2.7278732623717267E-4 2.273227718643105E-6 3.620115141939145E-4 3.409841577964658E-6 52-53 0.45131981157624623 2.7562886088547655E-4 2.273227718643105E-6 3.7565088050577316E-4 3.978148507625434E-6 54-55 0.45411929151175523 2.767654747447981E-4 2.273227718643105E-6 3.9270008839559646E-4 4.54645543728621E-6 56-57 0.457197241842798 2.790387024634412E-4 2.273227718643105E-6 4.114542170744021E-4 4.54645543728621E-6 58-59 0.46053263521297705 2.8244854404140587E-4 2.273227718643105E-6 4.250935833862607E-4 4.54645543728621E-6 60-61 0.4641135371767696 2.881316133380136E-4 2.273227718643105E-6 4.307766526828685E-4 4.54645543728621E-6 62-63 0.46834230904037544 3.0063436579055066E-4 2.273227718643105E-6 4.5350892986929954E-4 4.54645543728621E-6 64-65 0.4727358899135829 3.040442073685153E-4 2.273227718643105E-6 4.8476581100064225E-4 4.54645543728621E-6 66-67 0.4778910020725358 3.137054251727485E-4 2.273227718643105E-6 5.194325337099496E-4 5.683069296607763E-6 68-69 0.4839355145764078 3.171152667507132E-4 2.273227718643105E-6 5.325035930921475E-4 5.683069296607763E-6 70-71 0.49092682642509466 3.216617221879994E-4 2.273227718643105E-6 5.728533850980625E-4 6.25137622626854E-6 72-73 0.49862397548042026 3.2223002911766017E-4 2.273227718643105E-6 5.876293652692427E-4 6.819683155929316E-6 74-75 0.507552077345391 3.2279833604732095E-4 2.273227718643105E-6 6.569628106878574E-4 6.819683155929316E-6 76-77 0.5176679406933529 3.239349499066425E-4 2.273227718643105E-6 7.740340381979774E-4 6.819683155929316E-6 78-79 0.5295245281668657 3.2734479148460716E-4 2.273227718643105E-6 8.212035133598218E-4 6.819683155929316E-6 80-81 0.5428462109050439 3.2734479148460716E-4 2.273227718643105E-6 9.371381270106202E-4 6.819683155929316E-6 82-83 0.5585638756586719 3.313229399922326E-4 2.273227718643105E-6 0.0010473896713648109 7.95629701525087E-6 84-85 0.5778863112671384 3.341644746405365E-4 2.8415346483038817E-6 0.0010991056019639415 9.09291087457242E-6 86-87 0.6011931467594565 3.3984754393714424E-4 3.978148507625434E-6 0.0011263843345876588 9.661217804233197E-6 88-89 0.6283746988982017 3.432573855151089E-4 4.54645543728621E-6 0.0011428652355478211 1.0229524733893974E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 57775 0.0 68.34319 1 GCGTGTT 71965 0.0 54.159397 2 AGATCGG 79470 0.0 43.71725 1 ATCGGAA 83625 0.0 41.545155 3 GATCGGA 85430 0.0 40.84056 2 GTTTTTT 1197405 0.0 40.677055 1 CCGAAAC 166875 0.0 40.251472 1 CGGAAGA 88755 0.0 39.988525 5 TCGGAAG 89300 0.0 39.16306 4 CTCGCAT 15730 0.0 37.76765 1 CCTTTAG 94880 0.0 37.5522 5 CGTGTTT 106060 0.0 36.977127 3 TATGCCG 59295 0.0 36.33322 46-47 TGCCGTC 59250 0.0 35.642982 48-49 CGAAACC 190750 0.0 35.037834 2 CCGTCTT 60920 0.0 34.58408 50-51 TAACGGC 9455 0.0 34.55797 9 CCAAACG 198485 0.0 34.077225 7 TAACGAG 100790 0.0 33.954044 94-95 CGTCTGA 66190 0.0 33.724995 16-17 >>END_MODULE