FastQCFastQC Report
Fri 27 May 2016
SRR1233030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44704672
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA921600.20615294974091297No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT564670.12631118286697193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT281950.073.4898761
GCGTGTT391200.052.9428562
GTTTTTT6361900.039.6257551
CGTGTTT630350.034.002153
CCTTTAG362050.028.911145
TCGTCTA168300.028.4637244-45
TAGGCAT367400.028.3447349
CCCGAAA211750.028.3443831
TTTAGGC395050.027.2248047
TTAGGCA397450.026.4517578
ACTCGTC188650.025.45570442-43
CTAACGA182500.024.93358894-95
CCGAAAC246650.024.4431112
CGAAACC263600.023.1051043
CCAAACG284950.021.677528
CGCAATC117700.021.45414248-49
CGAGCTA225350.021.42391612-13
GTGTTTT1631750.021.2827591
TCAACGG122450.020.94823616-17
TATCGAC125000.020.4448870-71
Produced by FastQC (version 0.11.5)