FastQCFastQC Report
Fri 27 May 2016
SRR1233030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44704672
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA684450.15310480300582452No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT515400.11528996342932568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT284450.074.0040051
GCGTGTT398700.053.31162
CGTGTTT644250.034.0347943
GTTTTTT6119350.033.182261
CCCGAAA195800.027.4777181
TCGTCTA171150.027.38333944-45
GTGTTTT1774700.025.957031
ACTCGTC191000.024.4885742-43
CCGAAAC223300.024.4876792
CCTTTAG338850.024.4436445
CTAACGA186500.024.36719194-95
TTTAGGC365150.023.2704877
CGAAACC241350.022.7767013
CGCGTTT31500.022.7630161
CCAAACG278000.022.1311728
TCAACGG127800.021.71567216-17
CGAGCTA222950.021.33873412-13
CGCAATC131950.020.57574348-49
AACGGAC138300.020.28922518-19
CGCTTCG40200.020.0740014
Produced by FastQC (version 0.11.5)