##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233030_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44704672 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.27057525441636 31.0 30.0 34.0 23.0 34.0 2 29.951042208742745 31.0 30.0 34.0 26.0 34.0 3 27.764344473884073 31.0 27.0 34.0 15.0 34.0 4 31.19420565259935 35.0 30.0 37.0 19.0 37.0 5 32.35969054867464 35.0 32.0 37.0 19.0 37.0 6 32.91926528395064 35.0 33.0 37.0 27.0 37.0 7 33.31263600368212 36.0 35.0 37.0 28.0 37.0 8 33.3751559568539 37.0 35.0 37.0 28.0 37.0 9 34.88819441511617 39.0 35.0 39.0 28.0 39.0 10-11 34.7676931507293 39.0 35.0 39.0 27.5 39.0 12-13 34.70300936331665 39.0 35.0 39.0 27.0 39.0 14-15 35.77928601064336 40.0 36.0 41.0 27.0 41.0 16-17 35.57852632270739 40.0 36.0 41.0 27.0 41.0 18-19 35.27144397793591 40.0 35.5 41.0 25.5 41.0 20-21 34.93545316695311 39.5 35.0 41.0 23.0 41.0 22-23 34.6569368074102 39.0 34.5 41.0 19.5 41.0 24-25 34.34280768238273 39.0 34.0 41.0 16.5 41.0 26-27 33.94763795605077 39.0 34.0 41.0 9.0 41.0 28-29 33.610997705116816 39.0 33.5 41.0 4.5 41.0 30-31 33.334530258940276 39.0 33.0 41.0 2.0 41.0 32-33 33.09370063155815 39.0 33.0 41.0 2.0 41.0 34-35 33.08703526557582 39.0 33.0 41.0 2.0 41.0 36-37 33.01121546088069 39.0 33.0 41.0 2.0 41.0 38-39 32.92778649623019 39.0 33.0 41.0 2.0 41.0 40-41 32.81765379018998 39.0 33.0 41.0 2.0 41.0 42-43 32.71808015949653 39.0 33.0 41.0 2.0 41.0 44-45 32.58068099683183 39.0 32.5 41.0 2.0 41.0 46-47 32.46680628816603 39.0 32.0 41.0 2.0 41.0 48-49 32.46341961752901 39.0 32.0 41.0 2.0 41.0 50-51 32.43561675164511 39.0 32.0 41.0 2.0 41.0 52-53 32.30787229576363 39.0 32.0 41.0 2.0 41.0 54-55 32.17486765141683 39.0 32.0 41.0 2.0 41.0 56-57 32.03610697557517 38.0 32.0 41.0 2.0 41.0 58-59 31.855011742396858 38.0 31.0 40.0 2.0 41.0 60-61 31.649695673866034 38.0 31.0 40.0 2.0 41.0 62-63 31.407565198107257 38.0 31.0 40.0 2.0 41.0 64-65 30.966676849793238 37.0 30.0 40.0 2.0 41.0 66-67 30.78449382203274 37.0 30.0 40.0 2.0 41.0 68-69 30.412856054508126 36.0 29.0 39.5 2.0 41.0 70-71 30.180071335720797 36.0 29.0 39.0 2.0 41.0 72-73 29.91576514642586 35.5 29.0 39.0 2.0 41.0 74-75 29.591594934417593 35.0 29.0 38.5 2.0 40.0 76-77 28.460461179538463 34.0 27.0 36.5 2.0 39.0 78-79 28.903438437038528 35.0 28.5 37.0 2.0 39.0 80-81 28.742278156072814 35.0 29.0 37.0 2.0 39.0 82-83 28.43685825499402 35.0 29.0 36.0 2.0 39.0 84-85 28.12152209728773 34.5 28.0 36.0 2.0 37.0 86-87 27.7761964454185 34.0 27.5 35.5 2.0 37.0 88-89 27.501374431289868 34.0 27.0 35.0 2.0 37.0 90-91 27.238203570758778 34.0 26.0 35.0 2.0 36.0 92-93 26.991328333647097 34.0 26.0 35.0 2.0 36.0 94-95 26.748095243825972 34.0 25.5 35.0 2.0 36.0 96-97 26.465530638497917 34.0 25.0 35.0 2.0 35.5 98-99 26.151576785978882 34.0 24.5 35.0 2.0 35.0 100-101 25.235296603898583 33.0 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.2353411280932036 1101 2 1.8444337197369016 1101 3 4.040318690836894 1101 4 4.022197255405967 1101 5 2.6186204967126585 1101 6 2.028620550964966 1101 7 1.6913977498621549 1101 8 1.5591844870715192 1101 9 1.724324126080667 1101 10-11 1.6751474582245507 1101 12-13 1.634394562501221 1101 14-15 1.8737043836049239 1101 16-17 1.8959161678885437 1101 18-19 1.8492221266993525 1101 20-21 1.8669783721211672 1101 22-23 1.8674708379290976 1101 24-25 1.8615465007324943 1101 26-27 1.8602732782879414 1101 28-29 1.8529689604175417 1101 30-31 1.8112121513315316 1101 32-33 1.7969724350473868 1101 34-35 1.810508260824399 1101 36-37 1.8170145915879559 1101 38-39 1.8802871901111757 1101 40-41 1.8839909699698438 1101 42-43 1.8444269325184095 1101 44-45 1.876474545226749 1101 46-47 1.8147399491728464 1101 48-49 1.92525076111761 1101 50-51 1.8110429803818349 1101 52-53 1.7497983229580854 1101 54-55 1.72770754403156 1101 56-57 1.7288694070301673 1101 58-59 1.7892411309656353 1101 60-61 1.758979827982877 1101 62-63 1.7426488794654666 1101 64-65 1.354128915251291 1101 66-67 1.670500323794247 1101 68-69 1.5622163281089385 1101 70-71 1.6398914126376631 1101 72-73 1.6526061901486422 1101 74-75 1.7406569987791833 1101 76-77 1.5854928626521847 1101 78-79 1.768975803689564 1101 80-81 1.731323685232006 1101 82-83 1.7266177419462707 1101 84-85 1.746817058878662 1101 86-87 1.7380482826888084 1101 88-89 1.7850215439707178 1101 90-91 1.8511343875156072 1101 92-93 1.8188534205633715 1101 94-95 1.8079308006644652 1101 96-97 1.9467441665981369 1101 98-99 1.89578946892604 1101 100-101 1.9848021730751775 1102 1 2.178513623663683 1102 2 1.842260573004424 1102 3 4.041202936073244 1102 4 4.043482758365247 1102 5 2.6511549107115897 1102 6 1.9887041372436585 1102 7 1.643399182820474 1102 8 1.4809837812478222 1102 9 1.6235745179038403 1102 10-11 1.6640482711693991 1102 12-13 1.6802272230647333 1102 14-15 1.8650527696781296 1102 16-17 1.8067454369205507 1102 18-19 1.808509503019316 1102 20-21 1.8146930296123784 1102 22-23 1.8547907368081766 1102 24-25 1.9098316836509142 1102 26-27 1.8349759301542719 1102 28-29 1.9010469621847932 1102 30-31 1.8284835208231769 1102 32-33 1.8383594954392848 1102 34-35 1.8952713489492083 1102 36-37 1.8527711608846076 1102 38-39 1.8813117786969613 1102 40-41 1.9424854240653033 1102 42-43 1.9026657965630491 1102 44-45 1.9595881512807907 1102 46-47 1.9526704369322943 1102 48-49 1.9635906484294168 1102 50-51 1.9693154574531633 1102 52-53 2.0452049856023393 1102 54-55 2.0647763226510136 1102 56-57 2.0189661872323725 1102 58-59 2.0244963897272825 1102 60-61 1.9864163973913733 1102 62-63 2.01222392510736 1102 64-65 1.9424355943940164 1102 66-67 1.9468275423569033 1102 68-69 1.7486382009810058 1102 70-71 1.8371325587398566 1102 72-73 1.8890295466700024 1102 74-75 1.9663230882298244 1102 76-77 1.8917749764366185 1102 78-79 1.9270240762492072 1102 80-81 1.9168643466896498 1102 82-83 1.92092513874152 1102 84-85 1.8949197370235993 1102 86-87 1.8721551326083699 1102 88-89 1.8740640825383075 1102 90-91 1.8745374305627003 1102 92-93 1.93090405742986 1102 94-95 1.952548324660885 1102 96-97 2.027283673189846 1102 98-99 1.974209568003662 1102 100-101 2.0898476504785215 1103 1 2.130033298042246 1103 2 1.7522995814065787 1103 3 3.9573342400394615 1103 4 3.991640180806481 1103 5 2.5855509646371715 1103 6 1.9656767387146772 1103 7 1.552998077962755 1103 8 1.5012139606658508 1103 9 1.6506968664519661 1103 10-11 1.6478032934853033 1103 12-13 1.625548327180418 1103 14-15 1.7747532286883327 1103 16-17 1.821229447040956 1103 18-19 1.781806911272838 1103 20-21 1.8070511224685006 1103 22-23 1.814343651625741 1103 24-25 1.7494374610429446 1103 26-27 1.8007864842229466 1103 28-29 1.8355880544750818 1103 30-31 1.7931470055892405 1103 32-33 1.8140295375587385 1103 34-35 1.8019725554201784 1103 36-37 1.811236635690804 1103 38-39 1.853325258830374 1103 40-41 1.8521983419642964 1103 42-43 1.849462673040314 1103 44-45 1.8599931413589061 1103 46-47 1.8217925410928544 1103 48-49 1.8254189462875274 1103 50-51 1.800512874346552 1103 52-53 1.8356397330393008 1103 54-55 1.9085263713366203 1103 56-57 1.8848188442364702 1103 58-59 1.892319328740335 1103 60-61 1.9558625927887903 1103 62-63 1.9535864178965774 1103 64-65 1.827683270336177 1103 66-67 1.925961903848151 1103 68-69 1.8015891311727898 1103 70-71 1.8227130372791116 1103 72-73 1.7981710911385882 1103 74-75 1.8718960884455704 1103 76-77 1.7900336687632326 1103 78-79 1.8767198956875646 1103 80-81 1.888068974042259 1103 82-83 1.9057010260657314 1103 84-85 1.8963290286182328 1103 86-87 1.8426188329006195 1103 88-89 1.76240974658716 1103 90-91 1.7509475312606817 1103 92-93 1.69423699296998 1103 94-95 1.717717135235553 1103 96-97 1.7275584523080205 1103 98-99 1.7191131182508705 1103 100-101 1.8202759241244344 1104 1 2.0802598726590205 1104 2 1.682145219617201 1104 3 3.8982002400491638 1104 4 3.9259033074223773 1104 5 2.5122828050732906 1104 6 1.9395738282864343 1104 7 1.5237713974587592 1104 8 1.442099538979484 1104 9 1.530364825091013 1104 10-11 1.4926050849633938 1104 12-13 1.4847872144731014 1104 14-15 1.6509563923403405 1104 16-17 1.6677433849008878 1104 18-19 1.6320723486078634 1104 20-21 1.685408467670193 1104 22-23 1.6825274653248101 1104 24-25 1.6691645219888827 1104 26-27 1.7192261602597654 1104 28-29 1.7547146669936922 1104 30-31 1.7458924821668163 1104 32-33 1.697584997604423 1104 34-35 1.686920437316246 1104 36-37 1.7332608825649771 1104 38-39 1.749057634134246 1104 40-41 1.7637527385738707 1104 42-43 1.6813572976183977 1104 44-45 1.7645405878638982 1104 46-47 1.758280244989706 1104 48-49 1.7966624423705184 1104 50-51 1.7848753739353072 1104 52-53 1.7382154822690339 1104 54-55 1.7932343565024595 1104 56-57 1.776607163761831 1104 58-59 1.7540021671626178 1104 60-61 1.803035213222266 1104 62-63 1.8075109742080535 1104 64-65 1.7356895815725828 1104 66-67 1.8450319170937668 1104 68-69 1.7362717637040816 1104 70-71 1.7394562981577764 1104 72-73 1.8029546127217841 1104 74-75 1.8005408829738947 1104 76-77 1.7886702740655593 1104 78-79 1.8638685037705969 1104 80-81 1.7520809987082373 1104 82-83 1.7704071787178215 1104 84-85 1.845082409242064 1104 86-87 1.8440934452961422 1104 88-89 1.7870173338625719 1104 90-91 1.8453574919799038 1104 92-93 1.8078016545963713 1104 94-95 1.8356010903105577 1104 96-97 1.7852933245846145 1104 98-99 1.8961216273032484 1104 100-101 1.971134012406484 1105 1 1.8006420305023028 1105 2 1.4708114317336687 1105 3 3.0592325943585976 1105 4 3.518329451574342 1105 5 2.127939046240556 1105 6 1.5863418401102507 1105 7 1.3081482282705323 1105 8 1.2366814317285986 1105 9 1.2822870852561934 1105 10-11 1.292821000732097 1105 12-13 1.2557556221188761 1105 14-15 1.4238818493042587 1105 16-17 1.4330308039417758 1105 18-19 1.3717725565802823 1105 20-21 1.3688633137235158 1105 22-23 1.3770044622115734 1105 24-25 1.4535682104111132 1105 26-27 1.4283914999266827 1105 28-29 1.4389277225714707 1105 30-31 1.3725197542915595 1105 32-33 1.4185026480453118 1105 34-35 1.3859575408250606 1105 36-37 1.3729618671275645 1105 38-39 1.4020534666948024 1105 40-41 1.423526878168154 1105 42-43 1.3762389550872243 1105 44-45 1.4498816173235411 1105 46-47 1.5419766328230367 1105 48-49 1.4536533367071343 1105 50-51 1.4667498205621925 1105 52-53 1.477405933746823 1105 54-55 1.4423415881349868 1105 56-57 1.4943066230852224 1105 58-59 1.473659874183646 1105 60-61 1.449214586446395 1105 62-63 1.4770944181241923 1105 64-65 1.3904269541890066 1105 66-67 1.4431371120357603 1105 68-69 1.3850981631823487 1105 70-71 1.3889422575137083 1105 72-73 1.5057826897263205 1105 74-75 1.4823769800848048 1105 76-77 1.4075404523090889 1105 78-79 1.493917138777661 1105 80-81 1.5241773756601447 1105 82-83 1.5639893003953986 1105 84-85 1.5760116174238448 1105 86-87 1.5348394293000176 1105 88-89 1.5061516199344673 1105 90-91 1.5203352043179734 1105 92-93 1.615132572614094 1105 94-95 1.6263532779758592 1105 96-97 1.5754525482278439 1105 98-99 1.6470652644315074 1105 100-101 1.7443015548367313 1106 1 1.8945098488709817 1106 2 1.5074836444457382 1106 3 3.7224918749236835 1106 4 3.794317298163495 1106 5 2.361912263944056 1106 6 1.6796197014436842 1106 7 1.3644997551827558 1106 8 1.2499813698428355 1106 9 1.3001012199622224 1106 10-11 1.3003089129070204 1106 12-13 1.3104257357212603 1106 14-15 1.4499078750715242 1106 16-17 1.4367963720622683 1106 18-19 1.2814510674203774 1106 20-21 1.3275633514321257 1106 22-23 1.2622558400080308 1106 24-25 1.3236229072387289 1106 26-27 1.3309644967084253 1106 28-29 1.4159606988384468 1106 30-31 1.3505765971731734 1106 32-33 1.3652958758427118 1106 34-35 1.3768073695534184 1106 36-37 1.3273900397781588 1106 38-39 1.3478786736963642 1106 40-41 1.3615761477143664 1106 42-43 1.4798512345668868 1106 44-45 1.433687207562535 1106 46-47 1.340242063429315 1106 48-49 1.3635930313457294 1106 50-51 1.2949091215353477 1106 52-53 1.5090603516209455 1106 54-55 1.6242458378786253 1106 56-57 1.5336682485781665 1106 58-59 1.485746769479583 1106 60-61 1.5214653098271427 1106 62-63 1.5533429336477305 1106 64-65 1.4649759794048798 1106 66-67 1.5481544140904084 1106 68-69 1.4896381533488636 1106 70-71 1.4869874370826182 1106 72-73 1.4279230208673184 1106 74-75 1.458131144875022 1106 76-77 1.3547702788443914 1106 78-79 1.48652476599716 1106 80-81 1.4913540661616373 1106 82-83 1.545915134704071 1106 84-85 1.557291721372696 1106 86-87 1.5677794808664345 1106 88-89 1.5304199882085214 1106 90-91 1.551222044132036 1106 92-93 1.5937628097089238 1106 94-95 1.5346296731038152 1106 96-97 1.5346760744226025 1106 98-99 1.5608317821419675 1106 100-101 1.5351650562844235 1107 1 1.566891570849613 1107 2 1.2594507038564657 1107 3 2.189933749852937 1107 4 3.0270298492220995 1107 5 1.8835288036113482 1107 6 1.3668421617708262 1107 7 1.0507629963879594 1107 8 0.968750255444661 1107 9 1.0718145804950652 1107 10-11 0.904908361049074 1107 12-13 0.9556069862768268 1107 14-15 1.0298814689147804 1107 16-17 1.0553345018453513 1107 18-19 1.0383697310086575 1107 20-21 1.0134091644914989 1107 22-23 1.0155075185868938 1107 24-25 1.048441982980421 1107 26-27 1.0201437663699338 1107 28-29 1.0153582125811766 1107 30-31 1.000764159957754 1107 32-33 1.080282731388003 1107 34-35 1.071822542654992 1107 36-37 1.035038511956607 1107 38-39 1.1032930307454123 1107 40-41 1.1325255650244515 1107 42-43 1.0978400365057013 1107 44-45 1.11774926833629 1107 46-47 1.107852092259435 1107 48-49 1.163680746288577 1107 50-51 1.1375851012337606 1107 52-53 1.0936892672655887 1107 54-55 0.9545476923667664 1107 56-57 0.9878329231577467 1107 58-59 1.073793737868975 1107 60-61 1.1517376589674555 1107 62-63 1.1424906660320495 1107 64-65 1.0363101623096682 1107 66-67 1.1661074110781904 1107 68-69 1.0530823757819228 1107 70-71 1.1257029809236627 1107 72-73 1.1766911826251523 1107 74-75 1.13800338682832 1107 76-77 1.0223141033782248 1107 78-79 1.1196243104568708 1107 80-81 1.1753942721631034 1107 82-83 1.2023714514420014 1107 84-85 1.2010631407717725 1107 86-87 1.1927471657127846 1107 88-89 1.1731328290493046 1107 90-91 1.110371071900822 1107 92-93 1.1451114968691911 1107 94-95 1.1100910040431202 1107 96-97 1.104348465642797 1107 98-99 1.1369698429608839 1107 100-101 1.172626394247839 1108 1 1.4161963380262534 1108 2 1.1381842158427844 1108 3 1.5116232697625698 1108 4 2.7149504065810497 1108 5 1.6869485110940943 1108 6 1.2048824407236296 1108 7 0.9973090584068274 1108 8 0.8859395107617374 1108 9 0.8992256713632898 1108 10-11 0.9695274609276154 1108 12-13 0.9470726096220048 1108 14-15 0.9554362164219228 1108 16-17 0.9742333774694245 1108 18-19 0.9707677695529782 1108 20-21 0.9196845086339778 1108 22-23 0.935569465066898 1108 24-25 1.0151153944857612 1108 26-27 0.9997681604870436 1108 28-29 1.0094181237044353 1108 30-31 1.0138653055974203 1108 32-33 0.9970352713105086 1108 34-35 1.010615497855973 1108 36-37 0.979793005470178 1108 38-39 0.9791103287906964 1108 40-41 0.9333382901433893 1108 42-43 0.9599434226166679 1108 44-45 0.9982146097882278 1108 46-47 0.9667465971431568 1108 48-49 1.0111865143193484 1108 50-51 0.9495770091674984 1108 52-53 0.9460717478332654 1108 54-55 0.9807257223871275 1108 56-57 0.9428241845949827 1108 58-59 1.0170507700291154 1108 60-61 0.9083521859090311 1108 62-63 0.9616656637504981 1108 64-65 0.9377719612570345 1108 66-67 1.0421073768270013 1108 68-69 0.934292375011271 1108 70-71 0.8530254431262101 1108 72-73 0.8604458563394424 1108 74-75 0.9115068143823812 1108 76-77 0.8271404694258386 1108 78-79 0.9635904292399786 1108 80-81 1.009867109383805 1108 82-83 1.0145836227463754 1108 84-85 1.049839481493347 1108 86-87 1.0487989704454002 1108 88-89 1.0142267925074684 1108 90-91 0.9970460340470488 1108 92-93 1.031650639468765 1108 94-95 1.0152963932406678 1108 96-97 0.9842538256175395 1108 98-99 0.9727285407018051 1108 100-101 0.975890412674385 1109 1 1.072744976849755 1109 2 0.8055451919917651 1109 3 -0.9956202119431588 1109 4 1.782771077618019 1109 5 1.1344983806883562 1109 6 0.873505674477606 1109 7 0.6947675555577391 1109 8 0.6555884068060465 1109 9 0.6837698708399174 1109 10-11 0.6983526256916193 1109 12-13 0.6924075301487989 1109 14-15 0.7014083970715603 1109 16-17 0.7308244018208256 1109 18-19 0.7171369727502537 1109 20-21 0.6639540094407153 1109 22-23 0.6896491920825767 1109 24-25 0.6634190851385924 1109 26-27 0.6119582134831276 1109 28-29 0.5871704750727105 1109 30-31 0.6307542395993408 1109 32-33 0.6328309071999669 1109 34-35 0.6089071686085958 1109 36-37 0.6198089161594282 1109 38-39 0.6190618202470048 1109 40-41 0.6497552370473798 1109 42-43 0.5973678789756747 1109 44-45 0.6037640634878372 1109 46-47 0.6057933586705389 1109 48-49 0.570428284800137 1109 50-51 0.5597189528718829 1109 52-53 0.6134415943378855 1109 54-55 0.6204626589445681 1109 56-57 0.6095105030692167 1109 58-59 0.6392154173561124 1109 60-61 0.6339017414862482 1109 62-63 0.5887718354630636 1109 64-65 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-3.4436012161747698 2316 8 -3.1992054455432175 2316 9 -3.4449756342687756 2316 10-11 -3.456530645779683 2316 12-13 -3.6591672828275392 2316 14-15 -3.8328963231665654 2316 16-17 -3.883247019298196 2316 18-19 -3.887357893089952 2316 20-21 -3.9712167956964777 2316 22-23 -4.0120055260265985 2316 24-25 -3.9959094947103537 2316 26-27 -4.132614050119404 2316 28-29 -4.055839082637007 2316 30-31 -4.188095765251241 2316 32-33 -4.179696304191594 2316 34-35 -4.195390548596855 2316 36-37 -4.245672656191225 2316 38-39 -4.307395916518331 2316 40-41 -4.290810111891936 2316 42-43 -4.20449919880517 2316 44-45 -4.178412217839 2316 46-47 -4.283237483456816 2316 48-49 -4.238942560620778 2316 50-51 -4.261075738134668 2316 52-53 -4.306299573290538 2316 54-55 -4.274683404948476 2316 56-57 -4.32705767313422 2316 58-59 -4.291632378557672 2316 60-61 -4.343651329375078 2316 62-63 -4.321811509702439 2316 64-65 -4.220449816647044 2316 66-67 -4.348824923175872 2316 68-69 -4.247790394999193 2316 70-71 -4.228047859067612 2316 72-73 -4.2171843412397045 2316 74-75 -4.182550216186694 2316 76-77 -4.075767699045279 2316 78-79 -4.208082476470935 2316 80-81 -4.2087502069481815 2316 82-83 -4.346572091180182 2316 84-85 -4.267398327795615 2316 86-87 -4.192954547453091 2316 88-89 -4.173690562105261 2316 90-91 -4.205231790705664 2316 92-93 -4.213661220002155 2316 94-95 -4.19466173796026 2316 96-97 -4.148324183993051 2316 98-99 -4.165192871569932 2316 100-101 -4.076280975157815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1682770.0 3 352619.0 4 235363.0 5 251239.0 6 432412.0 7 402725.0 8 345731.0 9 460540.0 10 597477.0 11 532032.0 12 387290.0 13 287733.0 14 246457.0 15 230507.0 16 219916.0 17 211198.0 18 204959.0 19 199961.0 20 199839.0 21 209991.0 22 233272.0 23 268215.0 24 310369.0 25 362003.0 26 426197.0 27 501786.0 28 596212.0 29 704788.0 30 841269.0 31 1030319.0 32 1234763.0 33 1550722.0 34 2038521.0 35 2792004.0 36 4115692.0 37 6992245.0 38 9702676.0 39 3304495.0 40 8365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.674770935149166 18.43530990105349 11.693384362775625 44.19653480102172 2 14.45747895969986 19.316510637692456 54.09257566524249 12.133434737365183 3 21.03176765270928 21.633729898594897 36.804489710881576 20.530012737814243 4 21.46891957704129 29.310782753696817 32.98767216785019 16.232625501411704 5 20.824872012286786 32.81356503058427 32.75135958066188 13.61020337646707 6 15.785036570348954 35.190473968949895 34.490787249911236 14.53370221078992 7 13.32281424778663 19.109864482113878 52.53433534586357 15.032985924235925 8 13.348410719688816 21.595106652694557 46.24289233490328 18.813590292713343 9 15.162963008603004 20.738266303778566 46.067359843433096 18.03141084418534 10-11 18.29601654551647 32.05947110925802 34.12917640384077 15.51533594138474 12-13 17.13585737444088 26.078357858010687 39.794572041705365 16.991212725843063 14-15 17.965136995417865 27.1940294189466 38.20269468724224 16.63813889839329 16-17 18.757996158526883 27.489787822485397 36.29011078414014 17.462105234847584 18-19 19.127043820666117 28.051663244148862 35.17111322806774 17.65017970711728 20-21 19.255600180629916 28.156132682080127 34.48739538437186 18.100871752918106 22-23 19.134387541249378 29.13295237116234 34.36328977638984 17.369370311198445 24-25 19.094127371348225 29.21024326123083 33.540989566355755 18.15463980106519 26-27 19.089296469339306 29.516379333344172 33.119862810664536 18.274461386651982 28-29 19.295554184651657 29.721428495309933 32.53629065789279 18.446726662145622 30-31 19.016650619540695 29.95974790645552 32.389067192986545 18.63453428101724 32-33 19.252439504303208 30.080286609521 31.933309527806212 18.73396435836958 34-35 19.35159993598373 30.631670882864125 31.168122050510988 18.848607130641152 36-37 19.65872103781369 30.288383783506596 31.287824854684782 18.76507032399493 38-39 19.665563466276645 29.926701250975373 31.52692110720824 18.880814175539744 40-41 19.731487325183096 29.99361301765925 31.532566121164056 18.742333535993602 42-43 19.490954927506866 30.065789020142603 31.86772248793369 18.575533564416837 44-45 19.889803772144425 29.822512912548234 31.580545598570186 18.707137716737154 46-47 19.822828295226724 29.76774998121497 31.432751688404792 18.97667003515351 48-49 19.707096095076135 29.87560970728725 31.163491327726135 19.253802869910484 50-51 19.28139822676098 30.093906661265297 31.60825453720415 19.01644057476957 52-53 19.785272660274288 30.071815969597658 31.15820697616022 18.984704393967835 54-55 19.66248806146971 30.231121335651558 30.88477941435006 19.22161118852868 56-57 19.93132706605958 30.0785728543778 30.71909434316977 19.27100573639286 58-59 19.69431934501782 30.413415894600647 30.522533095563475 19.36973166481805 60-61 19.737978130654867 30.55481796955652 30.175087391980032 19.532116507808585 62-63 19.892329215188852 30.644623557347987 30.277965921819604 19.185081305643557 64-65 20.034986563470817 30.60241222592463 30.078011379079435 19.284589831525125 66-67 19.84589162558219 30.46591531628269 30.278907600259743 19.409285457875384 68-69 19.975132842391535 30.55614859568154 30.261761568635535 19.20695699329139 70-71 19.906867265331666 30.61900547246013 30.018390312756576 19.455736949451623 72-73 19.667142831913026 30.613017017964783 30.099369468510485 19.6204706816117 74-75 19.752189101947163 30.278147021223607 30.711959064256177 19.257704812573056 76-77 19.750183272481202 30.511259733133784 30.29576240009524 19.442794594289776 78-79 19.858519998484883 30.608019547786725 30.17904582560701 19.354414628121386 80-81 19.772164670453424 30.47714905200083 30.385278433754976 19.36540784379077 82-83 20.11091690481443 30.607437147810863 29.961888077286496 19.319757870088207 84-85 19.721827691296212 30.440772650439495 30.22752457665759 19.609875081606706 86-87 19.725580993107275 31.00838507865667 29.952304278240454 19.3137296499956 88-89 19.670713557968593 30.82479618253823 30.027890274713297 19.47659998477988 90-91 19.748844118300095 31.01375984393989 29.932156581352988 19.305239456407026 92-93 19.71693133869285 30.831326142568265 29.900833323805205 19.55090919493368 94-95 19.71028101666012 30.998624257840092 29.78269096007801 19.508403765421775 96-97 19.79910158514737 30.895487797478033 29.88425311451019 19.421157502864407 98-99 19.798365410487172 30.83362557164342 29.801544047469196 19.566464970400215 100-101 19.931641428544008 31.324425705358916 29.27168307828093 19.472249787816143 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5968.0 1 7071.5 2 8486.5 3 10787.5 4 15290.0 5 20860.5 6 27128.5 7 34127.5 8 42382.0 9 51798.5 10 61691.5 11 72057.5 12 83199.0 13 94353.0 14 105785.5 15 118042.5 16 131539.0 17 147955.5 18 168248.0 19 193494.0 20 228022.0 21 273572.0 22 339754.0 23 411394.5 24 490886.0 25 596198.5 26 738460.0 27 907156.5 28 1039510.5 29 1133163.0 30 1269102.0 31 1436678.5 32 1585871.0 33 1677439.5 34 1733933.0 35 1860671.0 36 1950326.0 37 1942869.5 38 1871452.5 39 1751388.5 40 1675162.0 41 1639872.0 42 1544077.0 43 1429612.5 44 1362702.5 45 1311571.0 46 1242530.5 47 1161285.0 48 1100815.5 49 1039200.5 50 944695.0 51 831425.5 52 751018.0 53 689401.5 54 614721.5 55 531308.5 56 451761.0 57 371619.5 58 300086.5 59 243488.5 60 188299.5 61 141343.0 62 105738.5 63 76205.5 64 54206.0 65 39606.5 66 30293.0 67 24192.0 68 18857.0 69 14152.0 70 13384.0 71 16200.5 72 16620.5 73 20425.0 74 21316.5 75 13402.5 76 8754.0 77 7604.5 78 6334.5 79 4065.0 80 2877.5 81 2193.0 82 1646.0 83 1285.5 84 1133.0 85 985.0 86 740.5 87 488.5 88 325.5 89 219.5 90 135.0 91 74.0 92 43.0 93 23.5 94 8.0 95 5.0 96 3.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0438857934132701 2 0.0036908893996582728 3 0.029820149446572384 4 0.00756520481796623 5 0.03143743007442265 6 0.12297372408861428 7 0.1457946050918347 8 0.1438529735773478 9 0.1610502812770889 10-11 0.16303217703957207 12-13 0.16920938375300013 14-15 0.18030889478397247 16-17 0.19320575710744506 18-19 0.18792890371726695 20-21 0.19072503205034141 22-23 0.1617985252190196 24-25 0.1740108953265556 26-27 0.18333989789702515 28-29 0.18896570810317095 30-31 0.20070721019941717 32-33 0.20217797370261437 34-35 0.20358498547981743 36-37 0.19502212207261022 38-39 0.21367006115155032 40-41 0.2339352808583407 42-43 0.22276083358804197 44-45 0.2518372128980166 46-47 0.24156535585363428 48-49 0.23921325269985202 50-51 0.24014604111176566 52-53 0.24153068386230414 54-55 0.245092951358641 56-57 0.2423773515215591 58-59 0.2510979165667517 60-61 0.25672484522423067 62-63 0.2602557960832371 64-65 0.42088106585369867 66-67 0.2233055193873249 68-69 0.3740403240180355 70-71 0.016773414644447007 72-73 0.0030276477590530136 74-75 0.023333131713839664 76-77 0.03827787842845598 78-79 0.01892643346091433 80-81 0.025464899954975622 82-83 0.04525254094247688 84-85 0.026068863674919708 86-87 0.016724202785784896 88-89 0.030925179363803405 90-91 0.00882905482451588 92-93 0.027829306073423377 94-95 0.02943316528527488 96-97 0.025250157298995504 98-99 0.02105820170205029 100-101 0.011699000945583495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.4704672E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.289852245914126 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38090901036055 26.19090084373874 2 9.315235739510191 6.388361142873261 3 4.028198044377115 4.1437894727691456 4 2.3407119521754662 3.2105066796136774 5 1.4513355579845126 2.4883040921265134 6 1.0052994007989833 2.068294074978189 7 0.7564331596793794 1.8156586895521125 8 0.5951644932342953 1.6326482028014673 9 0.4761328897381469 1.4693873794687062 >10 3.154451265519751 20.612382044425846 >50 0.29366754306929627 6.941181610773399 >100 0.179720374707322 11.669283033879129 >500 0.014076717771742077 3.277832823049434 >1k 0.007810995842393684 4.99929979029948 >5k 5.640932783417958E-4 1.3023813861733904 >10k+ 2.8876195253542007E-4 1.7897887334777862 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 68445 0.15310480300582452 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 51540 0.11528996342932568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.016331626367821244 0.0 0.0 1.1184513332297796E-5 0.0 2 0.01664702964379204 0.0 0.0 1.3421415998757356E-5 0.0 3 0.017013881681091407 0.0 0.0 1.3421415998757356E-5 2.2369026664595593E-6 4 0.019610925676850955 0.0 2.2369026664595593E-6 2.2369026664595593E-5 2.2369026664595593E-6 5 0.020514634354100617 0.0 2.2369026664595593E-6 2.2369026664595593E-5 2.2369026664595593E-6 6 0.021503345332675745 0.0 2.2369026664595593E-6 2.4605929331055154E-5 2.2369026664595593E-6 7 0.021964147281966412 0.0 2.2369026664595593E-6 3.131663733043383E-5 2.2369026664595593E-6 8 0.02391248950445269 0.0 2.2369026664595593E-6 3.355353999689339E-5 2.2369026664595593E-6 9 0.02471777446437813 0.0 2.2369026664595593E-6 4.2501150662731624E-5 2.2369026664595593E-6 10-11 0.027689499656769652 0.0 2.2369026664595593E-6 4.6974955995650746E-5 2.2369026664595593E-6 12-13 0.03195974684704095 0.0 2.2369026664595593E-6 5.144876132856986E-5 3.355353999689339E-6 14-15 0.03621657262131349 3.355353999689339E-6 2.2369026664595593E-6 5.48041153282592E-5 4.4738053329191185E-6 16-17 0.041834553668126676 4.4738053329191185E-6 2.2369026664595593E-6 6.0396371994408105E-5 4.4738053329191185E-6 18-19 0.04641237497503617 4.4738053329191185E-6 2.2369026664595593E-6 6.710707999378677E-5 4.4738053329191185E-6 20-21 0.05145659048790247 4.4738053329191185E-6 2.2369026664595593E-6 7.046243399347612E-5 4.4738053329191185E-6 22-23 0.05597625232548401 4.4738053329191185E-6 2.2369026664595593E-6 7.381778799316546E-5 4.4738053329191185E-6 24-25 0.059650364955143836 4.4738053329191185E-6 2.2369026664595593E-6 8.164694732577392E-5 4.4738053329191185E-6 26-27 0.0632540151508102 4.4738053329191185E-6 2.2369026664595593E-6 8.612075265869303E-5 4.4738053329191185E-6 28-29 0.066416995521184 6.710707999378678E-6 2.2369026664595593E-6 9.506836332453127E-5 4.4738053329191185E-6 30-31 0.06939766832424137 8.947610665838237E-6 2.2369026664595593E-6 1.0066061999068016E-4 4.4738053329191185E-6 32-33 0.07183701068201552 1.0066061999068018E-5 2.2369026664595593E-6 1.0289752265713972E-4 4.4738053329191185E-6 34-35 0.07545408229368063 1.1184513332297796E-5 2.2369026664595593E-6 1.0848977932328863E-4 6.710707999378678E-6 36-37 0.07773013075680323 1.1184513332297796E-5 2.2369026664595593E-6 1.1408203598943753E-4 8.947610665838237E-6 38-39 0.0795957075806305 1.1184513332297796E-5 2.2369026664595593E-6 1.1520048732266731E-4 8.947610665838237E-6 40-41 0.08164023661777453 1.1184513332297796E-5 2.2369026664595593E-6 1.1855584132235665E-4 8.947610665838237E-6 42-43 0.08388161308956701 1.3421415998757356E-5 2.2369026664595593E-6 1.3309570865434377E-4 8.947610665838237E-6 44-45 0.08584785053338496 1.6776769998446694E-5 2.2369026664595593E-6 1.375695139872629E-4 8.947610665838237E-6 46-47 0.08815297873117155 2.0132123998136035E-5 2.2369026664595593E-6 1.4539867331987135E-4 8.947610665838237E-6 48-49 0.09038652604363141 2.0132123998136035E-5 2.2369026664595593E-6 1.4651712465310113E-4 8.947610665838237E-6 50-51 0.09271178636541612 2.3487477997825373E-5 2.2369026664595593E-6 1.4763557598633092E-4 8.947610665838237E-6 52-53 0.09534685770650549 2.572438066428493E-5 2.2369026664595593E-6 1.5099092998602026E-4 8.947610665838237E-6 54-55 0.09800653497692591 2.9079734663974272E-5 2.2369026664595593E-6 1.5546473531893938E-4 1.0066061999068018E-5 56-57 0.10096483875331866 2.9079734663974272E-5 2.2369026664595593E-6 1.5882008931862872E-4 1.1184513332297796E-5 58-59 0.1039969603177046 2.9079734663974272E-5 2.2369026664595593E-6 1.6888615131769672E-4 1.3421415998757356E-5 60-61 0.1075267927253778 2.9079734663974272E-5 3.355353999689339E-6 1.7447840798384562E-4 1.4539867331987136E-5 62-63 0.11148499199367798 3.0198185997204053E-5 4.4738053329191185E-6 1.7671531065030518E-4 1.6776769998446694E-5 64-65 0.11565122320995891 3.131663733043383E-5 4.4738053329191185E-6 1.9125517798229233E-4 2.2369026664595593E-5 66-67 0.12043819491618235 3.131663733043383E-5 4.4738053329191185E-6 2.0020278864813054E-4 2.2369026664595593E-5 68-69 0.1259991349450008 3.131663733043383E-5 4.4738053329191185E-6 2.0579504531427945E-4 2.2369026664595593E-5 70-71 0.13272997506837764 3.355353999689339E-5 4.4738053329191185E-6 2.1026885064719857E-4 2.2369026664595593E-5 72-73 0.14016655798302244 3.579044266335295E-5 4.4738053329191185E-6 2.1026885064719857E-4 2.3487477997825373E-5 74-75 0.14887482006354952 3.579044266335295E-5 4.4738053329191185E-6 2.136242046468879E-4 2.4605929331055154E-5 76-77 0.15910976821393524 3.579044266335295E-5 4.4738053329191185E-6 2.4829619597701105E-4 2.4605929331055154E-5 78-79 0.17124496517947835 3.579044266335295E-5 4.4738053329191185E-6 2.617176119757684E-4 2.4605929331055154E-5 80-81 0.18560811720081516 3.579044266335295E-5 4.4738053329191185E-6 2.7178367397483644E-4 2.4605929331055154E-5 82-83 0.20259851140390875 3.579044266335295E-5 4.4738053329191185E-6 2.8967889530651293E-4 2.572438066428493E-5 84-85 0.2233894032373171 3.579044266335295E-5 4.4738053329191185E-6 2.9527115197266183E-4 2.684283199751471E-5 86-87 0.2485265969516564 3.579044266335295E-5 4.4738053329191185E-6 3.042187626385001E-4 2.684283199751471E-5 88-89 0.2782673363535695 3.579044266335295E-5 4.4738053329191185E-6 3.1204792197110854E-4 2.684283199751471E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 28445 0.0 74.004005 1 GCGTGTT 39870 0.0 53.3116 2 CGTGTTT 64425 0.0 34.034794 3 GTTTTTT 611935 0.0 33.18226 1 CCCGAAA 19580 0.0 27.477718 1 TCGTCTA 17115 0.0 27.383339 44-45 GTGTTTT 177470 0.0 25.95703 1 ACTCGTC 19100 0.0 24.48857 42-43 CCGAAAC 22330 0.0 24.487679 2 CCTTTAG 33885 0.0 24.443644 5 CTAACGA 18650 0.0 24.367191 94-95 TTTAGGC 36515 0.0 23.270487 7 CGAAACC 24135 0.0 22.776701 3 CGCGTTT 3150 0.0 22.763016 1 CCAAACG 27800 0.0 22.131172 8 TCAACGG 12780 0.0 21.715672 16-17 CGAGCTA 22295 0.0 21.338734 12-13 CGCAATC 13195 0.0 20.575743 48-49 AACGGAC 13830 0.0 20.289225 18-19 CGCTTCG 4020 0.0 20.074001 4 >>END_MODULE