FastQCFastQC Report
Fri 27 May 2016
SRR1233029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53434557
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1238090.23170211741439156No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1038850.1944153855341217No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA889240.16641665055817717No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC878120.1643356002745564No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT614330.11496867092956342No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT593140.11100307241248392No Hit
CCTGGATGGAAATTTGCATCAAACACTACAATAGTCAGTCGGATTTATTG586360.10973423060286623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT387150.075.489431
GCGTGTT557000.052.26682
CGTGTTT827900.036.2670943
GTTTTTT6987000.035.0518721
CGCGCGT15500.032.6259081
CCTTTAG451900.031.5411265
TCGTCTA337350.030.80516244-45
ACTCGTC347200.029.9137742-43
CTAACGA339950.029.6138494-95
TTTAGGC501650.028.95027
TTAGGCA507550.028.1368758
TAGGCAT517600.027.8928079
CCCGAAA419400.027.6531731
CTTTAGG566800.025.193916
GCCTAAC417550.024.38456392-93
GTCTATG444450.023.5560346-47
CCGAAAC508950.022.7758962
TAACGAG263350.021.43373794-95
CATTCCG363050.021.22275212-13
TAGAGGT468700.021.02833280-81
Produced by FastQC (version 0.11.5)