##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233029_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53434557 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.062474233668674 31.0 30.0 34.0 25.0 34.0 2 30.27844338636512 31.0 31.0 34.0 25.0 34.0 3 30.193675864104197 31.0 31.0 34.0 25.0 34.0 4 33.55996597857076 37.0 35.0 37.0 26.0 37.0 5 33.52643537402209 37.0 35.0 37.0 27.0 37.0 6 33.546384243440066 37.0 35.0 37.0 28.0 37.0 7 33.69992817569349 37.0 35.0 37.0 28.0 37.0 8 33.656284827064255 37.0 35.0 37.0 28.0 37.0 9 35.10106253898577 39.0 35.0 39.0 28.0 39.0 10-11 34.98734231108157 39.0 35.0 39.0 27.0 39.0 12-13 34.884295335320175 39.0 35.0 39.0 27.0 39.0 14-15 35.87127063671549 39.0 36.0 41.0 27.0 41.0 16-17 35.66079921837847 39.0 35.5 41.0 26.5 41.0 18-19 35.34332452686002 39.0 34.5 41.0 25.0 41.0 20-21 35.096886786204664 39.0 34.0 41.0 25.0 41.0 22-23 34.81220223272366 39.0 34.0 41.0 23.5 41.0 24-25 34.51046597055161 39.0 34.0 41.0 18.5 41.0 26-27 34.12486738684855 39.0 33.5 41.0 17.5 41.0 28-29 33.85586146994724 38.5 33.0 41.0 15.5 41.0 30-31 33.729557409823755 39.0 33.0 41.0 10.0 41.0 32-33 33.54716965277732 39.0 33.0 41.0 9.5 41.0 34-35 33.36319346672978 38.5 33.0 41.0 9.0 41.0 36-37 33.19354379601201 38.0 33.0 41.0 8.0 41.0 38-39 33.05316823343365 38.0 33.0 41.0 8.0 41.0 40-41 32.86063667562547 38.0 32.0 40.5 6.0 41.0 42-43 32.704194693707294 38.0 31.5 40.0 2.0 41.0 44-45 32.448029399775876 38.0 31.0 40.0 2.0 41.0 46-47 32.3715558828344 38.0 31.0 40.0 2.0 41.0 48-49 32.23940893717899 38.0 31.0 40.0 2.0 41.0 50-51 31.736213130764796 37.5 30.5 39.5 2.0 40.5 52-53 31.849098870605403 38.0 31.0 40.0 2.0 41.0 54-55 32.07416332655289 38.0 31.0 40.0 2.0 41.0 56-57 31.95191781977345 38.0 31.0 40.0 2.0 41.0 58-59 31.76711082305782 38.0 31.0 40.0 2.0 41.0 60-61 31.660356517973938 38.0 31.0 40.0 2.0 41.0 62-63 31.439179649229615 37.5 30.0 40.0 2.0 41.0 64-65 31.164142962764714 37.0 30.0 40.0 2.0 41.0 66-67 30.910152413165886 37.0 29.0 40.0 2.0 41.0 68-69 30.584974485705942 36.0 29.0 39.5 2.0 41.0 70-71 30.242422333547182 36.0 29.0 39.0 2.0 41.0 72-73 29.91905306710038 35.0 28.5 39.0 2.0 41.0 74-75 29.54625903420515 35.0 28.0 38.5 2.0 40.0 76-77 29.160320810744253 35.0 28.0 37.5 2.0 40.0 78-79 28.77663866475023 35.0 27.5 37.0 2.0 39.0 80-81 28.385096081174588 34.5 26.0 36.5 2.0 39.0 82-83 28.021580229438413 34.0 26.0 36.0 2.0 38.5 84-85 27.652294001426828 34.0 26.0 36.0 2.0 37.0 86-87 27.29134765728478 34.0 26.0 35.0 2.0 37.0 88-89 26.99280860511298 34.0 25.0 35.0 2.0 36.5 90-91 26.684929436207362 34.0 25.0 35.0 2.0 36.0 92-93 26.35536684621527 34.0 23.5 35.0 2.0 36.0 94-95 25.969685872009755 34.0 20.5 35.0 2.0 36.0 96-97 25.65038702388793 34.0 19.5 35.0 2.0 35.0 98-99 25.259951411218772 34.0 10.0 35.0 2.0 35.0 100-101 23.736551713154466 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2417471238640765 1101 2 1.3486202716746156 1101 3 1.408122390024964 1101 4 1.429967247494794 1101 5 1.387171203518669 1101 6 1.4647895608379429 1101 7 1.3746280131572917 1101 8 1.3311230814206354 1101 9 1.5301362442306043 1101 10-11 1.4293488035633928 1101 12-13 1.4464393228700345 1101 14-15 1.6332281248321365 1101 16-17 1.6411970653271624 1101 18-19 1.6723686932030901 1101 20-21 1.6114587946725152 1101 22-23 1.7334650411379044 1101 24-25 1.7133387291184405 1101 26-27 1.6147759530320442 1101 28-29 1.6395325698674554 1101 30-31 1.6273171510379854 1101 32-33 1.7024019789770222 1101 34-35 1.6528476033219306 1101 36-37 1.6003011600191073 1101 38-39 1.6526249113115057 1101 40-41 1.6923314235058342 1101 42-43 1.6685607922217898 1101 44-45 1.6894211359182378 1101 46-47 1.6702594123063292 1101 48-49 1.5595854929503261 1101 50-51 1.5831075399763783 1101 52-53 1.7487292827869005 1101 54-55 1.6898775422899774 1101 56-57 1.6787177091025995 1101 58-59 1.7788576340473483 1101 60-61 1.8209825082034214 1101 62-63 1.7741068645205758 1101 64-65 1.7011870639827649 1101 66-67 1.6916337814814355 1101 68-69 1.7401778488815438 1101 70-71 1.7188479840003126 1101 72-73 1.778052268916408 1101 74-75 1.7880513197087957 1101 76-77 1.7136301487749286 1101 78-79 1.7632316639470744 1101 80-81 1.7956169443053014 1101 82-83 1.7503073065902122 1101 84-85 1.820824293598296 1101 86-87 1.8877316309093999 1101 88-89 1.8326169041130633 1101 90-91 1.847678068933881 1101 92-93 1.7622040505604346 1101 94-95 1.9271735753989816 1101 96-97 2.0131026707407003 1101 98-99 2.0590554592882135 1101 100-101 2.179163829329376 1102 1 1.0800838003226865 1102 2 1.25480637161548 1102 3 1.3237033092958121 1102 4 1.3384494286923925 1102 5 1.3661609991232808 1102 6 1.3072970695509483 1102 7 1.3259023927069151 1102 8 1.32763343970705 1102 9 1.4033021461831154 1102 10-11 1.4531297599627848 1102 12-13 1.4798942493415268 1102 14-15 1.6693037186756357 1102 16-17 1.663403510877913 1102 18-19 1.6871447014784593 1102 20-21 1.7409166930815587 1102 22-23 1.7228559681977487 1102 24-25 1.7146809442140594 1102 26-27 1.690154512060687 1102 28-29 1.6946400157984343 1102 30-31 1.7555602538219972 1102 32-33 1.783401193265263 1102 34-35 1.7803901684286672 1102 36-37 1.7976632255704885 1102 38-39 1.7298976265871886 1102 40-41 1.7647762702118328 1102 42-43 1.7042234362020352 1102 44-45 1.8630275550086992 1102 46-47 1.801849591091404 1102 48-49 1.770586960187572 1102 50-51 1.705604465002363 1102 52-53 1.8631065037358816 1102 54-55 1.8707227014918288 1102 56-57 1.8262578058273817 1102 58-59 1.9718524681415985 1102 60-61 1.916192397937202 1102 62-63 1.9063572909990967 1102 64-65 1.9442187019553856 1102 66-67 1.92281401924825 1102 68-69 1.9191798513631682 1102 70-71 1.9461642716187662 1102 72-73 1.9112952928679867 1102 74-75 1.8693358314590292 1102 76-77 1.8202231709268268 1102 78-79 1.8010277531164256 1102 80-81 1.8543497390590815 1102 82-83 1.8331183111430853 1102 84-85 1.8840496109734417 1102 86-87 1.9060306995067933 1102 88-89 1.8519622824986932 1102 90-91 1.860945878645282 1102 92-93 1.881951843030354 1102 94-95 1.8940831270168381 1102 96-97 2.0011510916613133 1102 98-99 2.0836992782728636 1102 100-101 2.1647625254230576 1103 1 1.0434621033628382 1103 2 1.1441546200622454 1103 3 1.1976812164019144 1103 4 1.2148482831684149 1103 5 1.216890922030899 1103 6 1.2500393944175414 1103 7 1.2483102954897305 1103 8 1.2519893186808346 1103 9 1.320275834395865 1103 10-11 1.3407626698325572 1103 12-13 1.3595756870641367 1103 14-15 1.6151418827667428 1103 16-17 1.579097534011666 1103 18-19 1.6140110694703438 1103 20-21 1.6018688865292958 1103 22-23 1.5690343040473067 1103 24-25 1.6207447459017956 1103 26-27 1.5768416302932309 1103 28-29 1.6240492843023944 1103 30-31 1.668002904113564 1103 32-33 1.64061366715557 1103 34-35 1.629499860318262 1103 36-37 1.6642939661612104 1103 38-39 1.7092699491177683 1103 40-41 1.6650919968517357 1103 42-43 1.662510779892152 1103 44-45 1.7463157127385998 1103 46-47 1.8004501789722198 1103 48-49 1.7766191597841612 1103 50-51 1.6972997945898918 1103 52-53 1.7879857011443256 1103 54-55 1.8635452182584302 1103 56-57 1.8560905269845662 1103 58-59 2.0015050671674857 1103 60-61 1.8749016577712112 1103 62-63 1.8093766682319767 1103 64-65 1.8005580460749044 1103 66-67 1.7981494770886357 1103 68-69 1.7070397768250132 1103 70-71 1.906172222719487 1103 72-73 1.8547762289026828 1103 74-75 1.8199584904906878 1103 76-77 1.8468582163310465 1103 78-79 1.769772232883593 1103 80-81 1.853944201905243 1103 82-83 1.868320021908609 1103 84-85 1.8405956145080538 1103 86-87 1.8148099588128126 1103 88-89 1.833261746228409 1103 90-91 1.852668817550608 1103 92-93 1.8112679123766782 1103 94-95 1.8928523383030473 1103 96-97 1.9434457929212599 1103 98-99 1.9774645652490648 1103 100-101 2.053690688818623 1104 1 0.9244212050358946 1104 2 1.0398567520032636 1104 3 1.1045695250845817 1104 4 1.0711281519269562 1104 5 1.064612765888434 1104 6 1.0750374297030518 1104 7 1.061737492286916 1104 8 1.0771928405444555 1104 9 1.1075390827684046 1104 10-11 1.124300180385184 1104 12-13 1.2019991485218213 1104 14-15 1.373109632354506 1104 16-17 1.3112175387155034 1104 18-19 1.3732379716392558 1104 20-21 1.364161520258861 1104 22-23 1.3797902420543977 1104 24-25 1.4280172403873692 1104 26-27 1.4100063183317815 1104 28-29 1.4259371906044365 1104 30-31 1.4266990963756143 1104 32-33 1.4270272122415477 1104 34-35 1.4866518107371576 1104 36-37 1.4777807104812055 1104 38-39 1.455354551089691 1104 40-41 1.45467142727464 1104 42-43 1.462552967264763 1104 44-45 1.6044998575896159 1104 46-47 1.5069147893524288 1104 48-49 1.441788377929683 1104 50-51 1.326474527641718 1104 52-53 1.4510058444459268 1104 54-55 1.5132523802953415 1104 56-57 1.5780296487127856 1104 58-59 1.6425365534698173 1104 60-61 1.5354262394700626 1104 62-63 1.4481719804789606 1104 64-65 1.3823280199145316 1104 66-67 1.5384057671693725 1104 68-69 1.497035246718042 1104 70-71 1.475231428575686 1104 72-73 1.4823716003608602 1104 74-75 1.5359538709657947 1104 76-77 1.5289847407280455 1104 78-79 1.4409615206172965 1104 80-81 1.4825542518026964 1104 82-83 1.4140483502932746 1104 84-85 1.5075029851366715 1104 86-87 1.4343853260555726 1104 88-89 1.4248857036173916 1104 90-91 1.4312887624796886 1104 92-93 1.4741969123725447 1104 94-95 1.5111441397697227 1104 96-97 1.5389295571450035 1104 98-99 1.6735260838733517 1104 100-101 1.7187858510463911 1105 1 0.8973608793011572 1105 2 0.9956506277775432 1105 3 1.0588437358021707 1105 4 1.1217449682492528 1105 5 1.110603352959373 1105 6 1.123359167512568 1105 7 1.080320664426992 1105 8 1.0367218953214916 1105 9 1.1791575757145054 1105 10-11 1.1467592537713074 1105 12-13 1.0810209737472434 1105 14-15 1.2273308551205062 1105 16-17 1.2265005690088842 1105 18-19 1.2389663346983966 1105 20-21 1.2147918406422207 1105 22-23 1.2430387329984782 1105 24-25 1.270315289094313 1105 26-27 1.231047327450156 1105 28-29 1.2545403553728889 1105 30-31 1.2947418117936849 1105 32-33 1.2672657905151326 1105 34-35 1.2237498184878888 1105 36-37 1.2666723746386381 1105 38-39 1.281884830887293 1105 40-41 1.2661289352358551 1105 42-43 1.2734172485054174 1105 44-45 1.3614705432833603 1105 46-47 1.3015415312133243 1105 48-49 1.3279891165435274 1105 50-51 1.2475548285206024 1105 52-53 1.2459274907496756 1105 54-55 1.4262751422880058 1105 56-57 1.4116813240092085 1105 58-59 1.5263052562304793 1105 60-61 1.4636899479116536 1105 62-63 1.4405082792851829 1105 64-65 1.4184228536956027 1105 66-67 1.449118428743894 1105 68-69 1.400301758783968 1105 70-71 1.4894838440705307 1105 72-73 1.360459858500807 1105 74-75 1.455939192403573 1105 76-77 1.4209548119485156 1105 78-79 1.4158119196589531 1105 80-81 1.3969457496656865 1105 82-83 1.3690972663338563 1105 84-85 1.421340516674931 1105 86-87 1.3769422306316308 1105 88-89 1.365063736311047 1105 90-91 1.3168572099349092 1105 92-93 1.4166650429381171 1105 94-95 1.3685350525542361 1105 96-97 1.4651551828295268 1105 98-99 1.4892714671500507 1105 100-101 1.5765279055822106 1106 1 0.6617568165872143 1106 2 0.7580950913367843 1106 3 0.814861436686332 1106 4 0.8653589285679786 1106 5 0.8848211187440285 1106 6 0.8153896728612793 1106 7 0.8762613256937541 1106 8 0.8702554115693601 1106 9 0.9480528131867487 1106 10-11 0.8589234507355243 1106 12-13 0.8945130227268692 1106 14-15 1.0133633681865817 1106 16-17 1.0074952499914858 1106 18-19 1.0699761819444689 1106 20-21 1.0223501623537246 1106 22-23 1.0555287797940878 1106 24-25 1.0735909637811787 1106 26-27 1.0288478279038316 1106 28-29 0.9752690791439349 1106 30-31 0.974396835577835 1106 32-33 0.9883810500365158 1106 34-35 0.9902372791737832 1106 36-37 1.0144548821894332 1106 38-39 0.9672886740496409 1106 40-41 1.0611672011152393 1106 42-43 0.9951275571043894 1106 44-45 1.090769765866014 1106 46-47 1.0517218044332495 1106 48-49 1.0115428014097887 1106 50-51 0.9719578918952045 1106 52-53 1.0021593713832395 1106 54-55 1.0931075062113536 1106 56-57 1.0719577180178987 1106 58-59 1.1500533449507273 1106 60-61 1.1574375040309377 1106 62-63 1.1018489108322314 1106 64-65 1.1559279862517329 1106 66-67 1.1288199323044452 1106 68-69 1.131676031233603 1106 70-71 1.1364500079762117 1106 72-73 1.187403812101774 1106 74-75 1.187040300261554 1106 76-77 1.1645125951708373 1106 78-79 1.1564468517723903 1106 80-81 1.1714266590672615 1106 82-83 1.1330088904614826 1106 84-85 1.2039078047724807 1106 86-87 1.2011196739657493 1106 88-89 1.1864235582177507 1106 90-91 1.1810641395949304 1106 92-93 1.1997262421175954 1106 94-95 1.2027316280887348 1106 96-97 1.1546520540608967 1106 98-99 1.1999461294751583 1106 100-101 1.2811645786360764 1107 1 0.6312014027859725 1107 2 0.7175764620924703 1107 3 0.6787335995477086 1107 4 0.8056923708172903 1107 5 0.8259665027376144 1107 6 0.789763954089409 1107 7 0.7400190274763077 1107 8 0.7492213864135024 1107 9 0.859301203438001 1107 10-11 0.8947712066051636 1107 12-13 0.9060965354771966 1107 14-15 0.9783104379640477 1107 16-17 0.9701731936813403 1107 18-19 0.8976988324060073 1107 20-21 0.9781344828092671 1107 22-23 0.8557572274313117 1107 24-25 0.9343382533923048 1107 26-27 0.9395621145993402 1107 28-29 0.882673293426997 1107 30-31 0.8831102191538207 1107 32-33 0.8178325919433576 1107 34-35 0.8300148040571571 1107 36-37 0.9003696302177175 1107 38-39 0.85742152289329 1107 40-41 0.9166891278644371 1107 42-43 0.9611511606690541 1107 44-45 1.0207961847615863 1107 46-47 0.8511797878862595 1107 48-49 0.8012314736180315 1107 50-51 0.8481999985775239 1107 52-53 0.85527877214518 1107 54-55 1.038974947810317 1107 56-57 0.945604755670054 1107 58-59 1.0277047820234841 1107 60-61 1.0071686051950408 1107 62-63 0.9060004755623723 1107 64-65 0.9244586229431491 1107 66-67 0.9504603981037505 1107 68-69 0.9969882666585335 1107 70-71 1.0234390341515684 1107 72-73 1.0053880426378505 1107 74-75 1.060829750169134 1107 76-77 1.028723830158338 1107 78-79 1.0225068866529803 1107 80-81 1.0129990855445605 1107 82-83 1.0059041766759975 1107 84-85 1.024547113707456 1107 86-87 0.9821686392541302 1107 88-89 1.040250125513797 1107 90-91 1.0167498861825734 1107 92-93 1.0604486015477868 1107 94-95 0.9920487586491653 1107 96-97 1.0730404409877643 1107 98-99 1.1985123685435752 1107 100-101 1.2241130441080443 1108 1 0.39213306800094827 1108 2 0.4783968537856147 1108 3 0.5145210271057579 1108 4 0.5270596789529591 1108 5 0.5935049374125327 1108 6 0.5139580982853289 1108 7 0.5639904897610606 1108 8 0.5205911654834381 1108 9 0.5284801329946234 1108 10-11 0.5809024604680388 1108 12-13 0.4834075384809182 1108 14-15 0.5972845586613218 1108 16-17 0.5524675770619822 1108 18-19 0.6151448167742473 1108 20-21 0.5557532241219221 1108 22-23 0.5557763952733268 1108 24-25 0.623222539839567 1108 26-27 0.5368231457745196 1108 28-29 0.5421749921826589 1108 30-31 0.4906634676256516 1108 32-33 0.5407927015850476 1108 34-35 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2311 66-67 -0.28659303032573646 2311 68-69 -0.27681899748646543 2311 70-71 -0.19125725116744619 2311 72-73 -0.16185668045412527 2311 74-75 -0.22977971294027455 2311 76-77 -0.26451315316237256 2311 78-79 -0.19307283149057852 2311 80-81 -0.13752282857517173 2311 82-83 -0.1942564318986406 2311 84-85 -0.18945612788444066 2311 86-87 -0.12723511717669922 2311 88-89 -0.10387191170022092 2311 90-91 -0.05632308091798777 2311 92-93 -0.04867492587456823 2311 94-95 -0.043257250408846204 2311 96-97 -0.11208041757303278 2311 98-99 -0.04930530823553525 2311 100-101 0.07212456128053901 2312 1 0.15381830311554978 2312 2 0.23156680595821655 2312 3 0.12563991867677515 2312 4 0.08820356387352746 2312 5 0.07575315363486368 2312 6 0.0970392482436111 2312 7 0.1747262551781077 2312 8 0.13417058565904227 2312 9 0.058713586677640706 2312 10-11 0.03508614556454859 2312 12-13 -0.005449207563096081 2312 14-15 0.03697071141255748 2312 16-17 0.04890368599436812 2312 18-19 0.01469409977661229 2312 20-21 -0.10339061710557473 2312 22-23 -0.16815371030125448 2312 24-25 -9.764484388341543E-5 2312 26-27 0.02113243424221878 2312 28-29 -0.010692516477966763 2312 30-31 -0.17486178235709815 2312 32-33 -0.07866835459639532 2312 34-35 -0.00957878935231804 2312 36-37 0.012748561284624316 2312 38-39 0.0314974455493342 2312 40-41 0.1226674487957169 2312 42-43 0.09066488906743331 2312 44-45 0.12160976810292823 2312 46-47 0.07582282123022566 2312 48-49 0.030391689013214318 2312 50-51 -0.1066220590238629 2312 52-53 -0.1649641196513194 2312 54-55 -0.04982054175819428 2312 56-57 0.027291321242088884 2312 58-59 -0.08696364802170109 2312 60-61 -0.03651851774442605 2312 62-63 -0.008892314713634164 2312 64-65 -0.026248920479975624 2312 66-67 -0.057549920268471055 2312 68-69 -0.0492062823212045 2312 70-71 -0.017800451156080754 2312 72-73 -0.06577462167838632 2312 74-75 -2.4289777980968097E-4 2312 76-77 -0.05338688787324841 2312 78-79 -0.007560969295422382 2312 80-81 0.031014556167008323 2312 82-83 -0.1549436549385561 2312 84-85 -0.043136748812830916 2312 86-87 0.11226319224280346 2312 88-89 -0.1997676292331576 2312 90-91 -0.16398796621049172 2312 92-93 0.02916402440975574 2312 94-95 0.0859653374728957 2312 96-97 0.1087628692287872 2312 98-99 0.16046892190429318 2312 100-101 0.18536366122810222 2313 1 -0.6306053245737395 2313 2 -0.4907312234982051 2313 3 -1.0285932123246049 2313 4 -1.125122885734001 2313 5 -1.238743296194471 2313 6 -0.8891655260567646 2313 7 -0.7928544420769583 2313 8 -0.7637706538968558 2313 9 -0.9982420235948837 2313 10-11 -1.0774088488294638 2313 12-13 -1.0425802882044195 2313 14-15 -1.0866716136340742 2313 16-17 -1.249698051154077 2313 18-19 -1.0974023043071668 2313 20-21 -1.1706795286368745 2313 22-23 -1.1863915210669589 2313 24-25 -1.1438980212492567 2313 26-27 -1.1421214418487295 2313 28-29 -1.1492434065383677 2313 30-31 -1.1743254185451093 2313 32-33 -1.2418097500768184 2313 34-35 -1.2751404946702962 2313 36-37 -1.1935196547068827 2313 38-39 -1.1979874339809626 2313 40-41 -1.14509153494323 2313 42-43 -1.14767794383431 2313 44-45 -1.055441611781486 2313 46-47 -1.0916218563606819 2313 48-49 -1.1996516256734537 2313 50-51 -1.179276986205796 2313 52-53 -1.1745883368932653 2313 54-55 -1.3323055993565234 2313 56-57 -1.2304222519513566 2313 58-59 -1.3382354878577516 2313 60-61 -1.2572725894240762 2313 62-63 -1.2835429769506952 2313 64-65 -1.1852229107622776 2313 66-67 -1.350993597509735 2313 68-69 -1.2260981994886677 2313 70-71 -1.225373310535499 2313 72-73 -1.2536898873201103 2313 74-75 -1.3371724972163612 2313 76-77 -1.3239117798131055 2313 78-79 -1.3339167178803812 2313 80-81 -1.3388652141424053 2313 82-83 -1.233598491675611 2313 84-85 -1.1663301294390678 2313 86-87 -1.1463862161565572 2313 88-89 -1.2140138162039449 2313 90-91 -1.1963622416427988 2313 92-93 -1.1551952288808316 2313 94-95 -1.1299159928773541 2313 96-97 -1.1447798534606477 2313 98-99 -1.126233129702733 2313 100-101 -1.1094940173809995 2315 1 -2.5674410897653672 2315 2 -2.1863169595015606 2315 3 -2.251664305286578 2315 4 -2.6023394058178866 2315 5 -3.088714983060232 2315 6 -2.906130910997863 2315 7 -2.6206872261375764 2315 8 -2.5495543504557574 2315 9 -3.050196965343666 2315 10-11 -2.9643395771350356 2315 12-13 -3.0486481574945685 2315 14-15 -3.560264036746233 2315 16-17 -3.430248562802639 2315 18-19 -3.519951614654044 2315 20-21 -3.419960714531804 2315 22-23 -3.678643968127922 2315 24-25 -3.5844878972951406 2315 26-27 -3.775769754663038 2315 28-29 -3.971420225944769 2315 30-31 -3.970538508659388 2315 32-33 -4.036716630305968 2315 34-35 -3.6461328825205293 2315 36-37 -3.5163187386126395 2315 38-39 -3.617878791264939 2315 40-41 -3.6307249435751103 2315 42-43 -3.540874887850329 2315 44-45 -3.388532128463744 2315 46-47 -3.4483055293480973 2315 48-49 -3.4794952535416286 2315 50-51 -3.48323875916099 2315 52-53 -3.492825956952995 2315 54-55 -3.478413051906486 2315 56-57 -3.4163396269140307 2315 58-59 -3.566780457449081 2315 60-61 -3.775769352348121 2315 62-63 -3.774377050310367 2315 64-65 -3.4958375128700254 2315 66-67 -3.59025227014007 2315 68-69 -3.632459412710844 2315 70-71 -3.6620644162033322 2315 72-73 -3.6369482421323482 2315 74-75 -3.4787375419715794 2315 76-77 -3.681765286473567 2315 78-79 -3.7017419815955073 2315 80-81 -3.5773660824018165 2315 82-83 -3.657174717831669 2315 84-85 -3.5386952693545553 2315 86-87 -3.511129853135035 2315 88-89 -3.6362667318951765 2315 90-91 -3.467958436682533 2315 92-93 -3.38941786134453 2315 94-95 -3.387572739105128 2315 96-97 -3.4385341991685756 2315 98-99 -3.530332983473837 2315 100-101 -3.4853526567004423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 914195.0 3 252144.0 4 241902.0 5 250911.0 6 298786.0 7 392780.0 8 516456.0 9 687106.0 10 810818.0 11 743656.0 12 575080.0 13 462908.0 14 395299.0 15 356269.0 16 336138.0 17 329668.0 18 332281.0 19 346835.0 20 372287.0 21 404112.0 22 446852.0 23 496839.0 24 553822.0 25 617474.0 26 691592.0 27 783160.0 28 892070.0 29 1029320.0 30 1198249.0 31 1412780.0 32 1692990.0 33 2078715.0 34 2635295.0 35 3508346.0 36 4984357.0 37 7897494.0 38 9989232.0 39 3497073.0 40 9266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.373908960338476 16.526248190998537 11.590355143939869 46.50948770472312 2 14.479163738228335 19.279491755851026 53.0544051263113 13.186939379609347 3 19.66032215583855 23.294485460172464 35.044328466628905 22.00086391736008 4 20.975268913535608 30.590973053418573 30.820567464711495 17.613190568334318 5 21.023652883646353 33.25462279330441 31.172916135496138 14.548808187553108 6 16.587325149228136 35.59692932830036 32.44329315808628 15.37245236438522 7 13.403980983749555 19.054637272466763 51.137209584520505 16.40417215926318 8 14.4732014833697 21.56801586985038 43.54355018772927 20.41523245905065 9 15.475225489600655 22.095834030991654 43.22612705647786 19.202813422929836 10-11 18.85164304124164 33.31349246988893 31.663940783962012 16.170923704907423 12-13 17.624185918735233 26.212690637641533 38.24345511026838 17.91966833335486 14-15 18.32545464151371 27.834438800284207 36.69072147192035 17.149385086281736 16-17 19.32636287774122 27.489369748831326 34.96256019558859 18.221707177838866 18-19 19.461812657920134 28.26099101095545 34.18532014761035 18.09187618351407 20-21 19.457007738739936 28.2553760107014 33.494303060311495 18.79331319024717 22-23 18.989354338156325 29.631341110785165 33.51977513210541 17.859529418953098 24-25 18.679885763610496 29.72537604485108 32.94426747768014 18.650470713858287 26-27 19.067483877843497 29.73800142780193 32.44627381716112 18.748240877193457 28-29 19.082247540841536 29.636206101618896 32.43617294216353 18.845373415376045 30-31 18.772589597204178 30.103206848277836 32.07165818634465 19.052545368173334 32-33 18.996611337955986 30.005743233037897 31.830193632144123 19.167451796861993 34-35 19.437660268259425 30.572745864461425 30.755641842679392 19.23395202459976 36-37 19.76553412395796 30.24396550003341 31.00816746600174 18.982332910006896 38-39 19.38890756191701 30.393140641545052 31.15263991316583 19.065311883372107 40-41 19.638192227188505 30.167143760970998 30.958189625767098 19.2364743860734 42-43 19.559980179081478 30.086447783641475 31.421900477924787 18.93167155935226 44-45 19.578718400581803 29.902612979943655 31.41683942318143 19.101829196293107 46-47 19.407504607032823 29.800743108928145 31.568970040275573 19.222782243763454 48-49 19.567893812247625 29.80390170985674 31.108276206717882 19.519928271177754 50-51 19.242115147720376 30.017188615279522 31.838959174021365 18.901737062978732 52-53 19.818479741266483 30.071896464948612 31.069075825539965 19.04054796824494 54-55 19.407345345339316 30.47102659039641 30.741562079133804 19.380065985130468 56-57 19.593341671751833 30.28978338958006 30.648587979231927 19.468286959436178 58-59 19.279755223851698 30.685308143557467 30.65474902721757 19.38018760537327 60-61 19.29410452987672 30.838071571997073 30.096776434429056 19.771047463697155 62-63 19.61927485903333 30.702235093715014 30.432268270947326 19.24622177630433 64-65 20.051601650582132 30.488636850620725 30.229741530415893 19.23001996838125 66-67 19.61959638242109 30.927866830091055 30.021950044312028 19.430586743175827 68-69 19.742304176883604 30.633561119413912 30.415694377595916 19.208440326106572 70-71 19.828747592509718 30.807201136393676 30.002902541900976 19.361148729195634 72-73 19.613265090625774 30.904345729548066 30.05363446139619 19.428754718429968 74-75 19.400880689344223 30.39997656675824 30.93561479834696 19.263527945550575 76-77 19.265135671919346 30.876669182084367 30.360703516927103 19.497491629069184 78-79 19.67258672319237 30.917152180386026 30.191775956171814 19.21848514024979 80-81 19.466999936292968 31.10509331618844 30.07759033000335 19.350316417515234 82-83 19.871516942564107 31.15346832404045 29.643164510509717 19.33185022288572 84-85 19.544928052370942 30.77115946191327 29.91217442464338 19.77173806107241 86-87 19.545936340891974 31.363684715637707 29.864081991947117 19.226296951523203 88-89 19.561342248120766 31.07417986937744 30.054498192245692 19.3099796902561 90-91 19.451596038186576 31.4818228219024 29.92895876697536 19.137622372935663 92-93 19.685826253265468 30.920737297848973 29.962666286621964 19.430770162263602 94-95 19.638814540053065 31.249896721826175 29.61279012642298 19.498498611697784 96-97 19.871398150906682 31.172107423587853 29.662320699930405 19.29417372557506 98-99 19.771148670476098 31.138515373062187 29.705486273757863 19.38484968270385 100-101 20.06530812736873 31.93609414415526 28.650739233309363 19.347858495166644 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15057.0 1 21817.0 2 25431.0 3 24923.0 4 31046.5 5 38788.0 6 46109.0 7 53570.0 8 63272.0 9 73576.0 10 83391.0 11 92696.5 12 101972.0 13 111213.0 14 120936.0 15 131571.5 16 142049.5 17 154898.5 18 173189.0 19 196782.5 20 233733.5 21 312097.0 22 455273.0 23 524252.0 24 556078.0 25 695890.0 26 876106.5 27 1083165.5 28 1245098.0 29 1309340.0 30 1418278.5 31 1582557.5 32 1729015.0 33 1876405.5 34 2020031.5 35 2205884.5 36 2317288.0 37 2375548.0 38 2391458.5 39 2141635.0 40 1957578.0 41 1938711.0 42 1793948.5 43 1652495.0 44 1566227.5 45 1479615.5 46 1415619.5 47 1400088.0 48 1370864.5 49 1271636.5 50 1179226.5 51 1118651.0 52 1011902.5 53 892961.0 54 800322.0 55 687974.0 56 583512.0 57 488066.0 58 408101.5 59 337783.5 60 265451.0 61 202192.5 62 150356.5 63 106766.5 64 72876.0 65 50037.0 66 34268.5 67 24322.0 68 17305.0 69 12100.5 70 10085.5 71 11205.0 72 10953.5 73 13359.5 74 14014.5 75 9252.0 76 7128.0 77 6373.0 78 5485.0 79 3799.5 80 2497.0 81 1688.5 82 1093.0 83 682.5 84 479.0 85 400.5 86 344.0 87 258.5 88 170.0 89 114.5 90 99.0 91 84.0 92 55.5 93 30.5 94 20.0 95 14.0 96 6.0 97 5.0 98 4.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6408250001960342 2 0.2799967069999289 3 0.3055288733843157 4 0.27367869822519536 5 0.30662367052093276 6 0.19330935970892393 7 0.09181137217999207 8 0.10547481473459207 9 0.10031523233176613 10-11 0.0884511871222213 12-13 0.09197512388846042 14-15 0.09150726186426511 16-17 0.08256922575403779 18-19 0.10464014888342762 20-21 0.09664438688992968 22-23 0.08951884826143501 24-25 0.1037137820755209 26-27 0.1008776024848489 28-29 0.09373241365133803 30-31 0.1040328639760221 32-33 0.10170291109552942 34-35 0.09838951224017821 36-37 0.10504157450018721 38-39 0.09537648080436037 40-41 0.10488811575625115 42-43 0.0851649242642734 44-45 0.3489212046803345 46-47 0.07352825999848749 48-49 0.07091010411109051 50-51 0.06543986132419886 52-53 0.23428752296009492 54-55 0.25798379876153926 56-57 0.2019255067465049 58-59 0.2610576522605025 60-61 0.07959268755610718 62-63 0.06660109486825164 64-65 0.10558616589635057 66-67 0.09897714694256751 68-69 0.09159241275266865 70-71 0.10060904968296079 72-73 0.12556761722568413 74-75 0.10740708489451872 76-77 0.0993701510428916 78-79 0.0944454353762117 80-81 0.09273773898789879 82-83 0.09970607597626384 84-85 0.09441268503451802 86-87 0.1155703414926786 88-89 0.12505671189526282 90-91 0.14480329648845033 92-93 0.14865005805138426 94-95 0.11096002910625795 96-97 0.0962317325845894 98-99 0.11489007010949862 100-101 0.11178159482074493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.3434557E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.375316871174892 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6233884322722 21.249648549477428 2 8.269513910682726 4.527611273510566 3 3.611708554595349 2.9661499838514223 4 2.060430869171388 2.2561979173886813 5 1.3734404508457994 1.8799183772796528 6 1.0255690306867549 1.6845166312988162 7 0.751956653666421 1.440953616725461 8 0.5828133385523889 1.276375985569518 9 0.4717910967970592 1.1623887694636776 >10 3.5197793763747334 19.068884614163938 >50 0.39684153117394716 7.551819438393878 >100 0.2714735848149399 14.211522044120839 >500 0.023815034121520678 4.48033433906262 >1k 0.01515933995014497 8.054894320175038 >5k 0.0014579261698674955 2.77020876168107 >10k+ 8.608701249630046E-4 5.418575377837872 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 123809 0.23170211741439156 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 103885 0.1944153855341217 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 88924 0.16641665055817717 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 87812 0.1643356002745564 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61433 0.11496867092956342 No Hit CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT 59314 0.11100307241248392 No Hit CCTGGATGGAAATTTGCATCAAACACTACAATAGTCAGTCGGATTTATTG 58636 0.10973423060286623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01903262714426546 0.0 0.0 3.7428961935625292E-6 0.0 2 0.019128070997201305 1.8714480967812646E-6 0.0 3.7428961935625292E-6 0.0 3 0.01924971512349209 3.7428961935625292E-6 0.0 5.614344290343794E-6 0.0 4 0.020514814036916223 3.7428961935625292E-6 0.0 1.8714480967812646E-5 0.0 5 0.020810502836207664 3.7428961935625292E-6 0.0 1.8714480967812646E-5 0.0 6 0.021364451472854918 3.7428961935625292E-6 0.0 3.1814617645281495E-5 0.0 7 0.021656397375952794 3.7428961935625292E-6 0.0 3.1814617645281495E-5 0.0 8 0.022350704619858643 3.7428961935625292E-6 0.0 4.304330622596908E-5 0.0 9 0.022874710086957396 3.7428961935625292E-6 0.0 4.678620241953161E-5 1.8714480967812646E-6 10-11 0.024294203468365985 5.614344290343794E-6 0.0 4.865765051631287E-5 1.8714480967812646E-6 12-13 0.0268000724699561 5.614344290343794E-6 0.0 5.427199480665667E-5 3.7428961935625292E-6 14-15 0.029275062577949325 6.550068338734426E-6 0.0 5.707916695182857E-5 5.614344290343794E-6 16-17 0.03287292154401131 7.4857923871250585E-6 0.0 7.111502767768805E-5 5.614344290343794E-6 18-19 0.03602069724279739 7.4857923871250585E-6 0.0 7.298647577446932E-5 5.614344290343794E-6 20-21 0.03937994657651976 1.216441262907822E-5 0.0 7.392219982285995E-5 5.614344290343794E-6 22-23 0.042458478695724944 1.3100136677468852E-5 0.0 8.42151643551569E-5 5.614344290343794E-6 24-25 0.04513184230197698 1.3100136677468852E-5 0.0 9.450812888745386E-5 5.614344290343794E-6 26-27 0.0479670861686006 1.3100136677468852E-5 0.0 1.1696550604882904E-4 5.614344290343794E-6 28-29 0.05056091323073943 1.3100136677468852E-5 0.0 1.2819419462951662E-4 5.614344290343794E-6 30-31 0.052962916862958176 1.3100136677468852E-5 0.0 1.4223005535537612E-4 5.614344290343794E-6 32-33 0.05536959911541889 1.4971584774250117E-5 0.0 1.506515717908918E-4 5.614344290343794E-6 34-35 0.05830777262736547 1.4971584774250117E-5 0.0 1.6936605275870445E-4 5.614344290343794E-6 36-37 0.06057128910042241 1.4971584774250117E-5 0.0 1.9930922230720468E-4 5.614344290343794E-6 38-39 0.06298077852502829 1.5907308822640748E-5 0.0 2.4422397662995503E-4 5.614344290343794E-6 40-41 0.0655156549721185 1.8714480967812646E-5 0.0 2.8726728285592413E-4 5.614344290343794E-6 42-43 0.06834996311469374 1.8714480967812646E-5 0.0 3.2563196883994E-4 5.614344290343794E-6 44-45 0.07130778683165653 1.8714480967812646E-5 0.0 3.5838231053361214E-4 5.614344290343794E-6 46-47 0.07452293466192673 1.8714480967812646E-5 0.0 4.163972015338314E-4 5.614344290343794E-6 48-49 0.07792897019806863 1.9650205016203277E-5 0.0 4.285616141629096E-4 5.614344290343794E-6 50-51 0.081728945558583 2.1521653112984545E-5 0.0 4.4166175084037846E-4 5.614344290343794E-6 52-53 0.08617082761629333 2.432882525815644E-5 0.0 4.5476188751784733E-4 5.614344290343794E-6 54-55 0.09075868262555259 2.7135997403328335E-5 0.0 4.744120925340506E-4 5.614344290343794E-6 56-57 0.09563380491766779 2.9007445500109603E-5 0.0 4.968694696954258E-4 5.614344290343794E-6 58-59 0.10118451997272103 3.1814617645281495E-5 0.0 5.277483632923166E-4 6.550068338734426E-6 60-61 0.10720871139625993 3.1814617645281495E-5 0.0 5.586272568892075E-4 8.42151643551569E-6 62-63 0.1139627675775435 3.368606574206276E-5 0.0 6.278708364701143E-4 9.357240483906323E-6 64-65 0.12157113981500772 3.368606574206276E-5 0.0 6.64364074357349E-4 9.357240483906323E-6 66-67 0.1300955858958464 3.4621789790453394E-5 0.0 7.317362058414745E-4 9.357240483906323E-6 68-69 0.1398027871738508 3.5557513838844024E-5 0.0 7.682294437287091E-4 9.357240483906323E-6 70-71 0.15075356571216636 3.5557513838844024E-5 0.0 8.234371625837564E-4 9.357240483906323E-6 72-73 0.16334092561111718 4.117185812918782E-5 0.0 8.739662611968505E-4 9.357240483906323E-6 74-75 0.17844912609643232 4.117185812918782E-5 0.0 9.095237750356946E-4 9.357240483906323E-6 76-77 0.19572352775377178 4.117185812918782E-5 0.0 9.460170129229292E-4 9.357240483906323E-6 78-79 0.21558146350871776 4.117185812918782E-5 0.0 9.609885976971794E-4 9.357240483906323E-6 80-81 0.23889783534651554 4.210758217757845E-5 0.0 9.89996043197289E-4 9.357240483906323E-6 82-83 0.26679644784928225 4.304330622596908E-5 0.0 0.0010143248684554456 9.357240483906323E-6 84-85 0.30041233428771574 4.304330622596908E-5 0.0 0.0010695325873104928 9.357240483906323E-6 86-87 0.34169086495842005 4.304330622596908E-5 0.0 0.0011228688580687588 9.357240483906323E-6 88-89 0.39182883091928694 4.491475432275035E-5 0.0 0.0012229913312465563 9.357240483906323E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 38715 0.0 75.48943 1 GCGTGTT 55700 0.0 52.2668 2 CGTGTTT 82790 0.0 36.267094 3 GTTTTTT 698700 0.0 35.051872 1 CGCGCGT 1550 0.0 32.625908 1 CCTTTAG 45190 0.0 31.541126 5 TCGTCTA 33735 0.0 30.805162 44-45 ACTCGTC 34720 0.0 29.91377 42-43 CTAACGA 33995 0.0 29.61384 94-95 TTTAGGC 50165 0.0 28.9502 7 TTAGGCA 50755 0.0 28.136875 8 TAGGCAT 51760 0.0 27.892807 9 CCCGAAA 41940 0.0 27.653173 1 CTTTAGG 56680 0.0 25.19391 6 GCCTAAC 41755 0.0 24.384563 92-93 GTCTATG 44445 0.0 23.55603 46-47 CCGAAAC 50895 0.0 22.775896 2 TAACGAG 26335 0.0 21.433737 94-95 CATTCCG 36305 0.0 21.222752 12-13 TAGAGGT 46870 0.0 21.028332 80-81 >>END_MODULE