FastQCFastQC Report
Fri 27 May 2016
SRR1233029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53434557
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA995120.1862315430068972No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT968030.18116179011271677No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA876500.16403242568287785No Hit
CCTGGATGGAAATTTGCATCAAACACTACAATAGTCAGTCGGATTTATTG633810.11861425182209333No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC613960.11489942734998251No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT548780.10270132865516222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT392050.074.4389651
GCGTGTT551800.053.0368042
CGTGTTT833650.036.3361053
GTTTTTT6749400.030.8956241
TCGTCTA335800.030.55401444-45
ACTCGTC341950.030.04629542-43
CTAACGA345200.028.95922394-95
CCTTTAG428750.028.097855
CGCGCGT18200.027.9165421
CCCGAAA372650.027.1411171
TTTAGGC476650.025.4576637
TTAGGCA477550.025.1114488
TAGGCAT483950.024.7501419
GCCTAAC420550.023.75986592-93
GTCTATG441300.023.41598346-47
CTTTAGG539200.022.3336816
CCGAAAC459200.022.0260732
CGAGCTA482000.021.7833912-13
TAACGAG274650.021.7265794-95
TCGACTC37250.021.406633
Produced by FastQC (version 0.11.5)