##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233029_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53434557 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.714767860805882 31.0 30.0 34.0 25.0 34.0 2 30.12526837267501 31.0 30.0 34.0 26.0 34.0 3 28.990655373076265 31.0 28.0 34.0 16.0 34.0 4 32.03846166442439 35.0 32.0 37.0 19.0 37.0 5 32.76523505191594 35.0 33.0 37.0 25.0 37.0 6 33.143171730608714 35.0 33.0 37.0 27.0 37.0 7 33.420500594774275 37.0 35.0 37.0 28.0 37.0 8 33.434283098856795 37.0 35.0 37.0 28.0 37.0 9 34.915623030242394 39.0 35.0 39.0 28.0 39.0 10-11 34.78721103648337 39.0 35.0 39.0 27.5 39.0 12-13 34.68531919334524 39.0 35.0 39.0 27.0 39.0 14-15 35.78440002786961 40.0 36.0 41.0 27.0 41.0 16-17 35.613517072856055 40.0 36.0 41.0 27.0 41.0 18-19 35.36935167255153 39.5 35.5 41.0 26.0 41.0 20-21 35.11911463025697 39.0 35.0 41.0 25.0 41.0 22-23 34.90676177029034 39.0 35.0 41.0 23.0 41.0 24-25 34.618709227813 39.0 34.0 41.0 18.5 41.0 26-27 34.24552064687277 39.0 34.0 41.0 16.5 41.0 28-29 33.99019127266274 39.0 34.0 41.0 10.5 41.0 30-31 33.71247672175892 39.0 34.0 41.0 8.5 41.0 32-33 33.44831512311406 39.0 33.0 41.0 4.5 41.0 34-35 33.24817625979382 39.0 33.0 41.0 2.0 41.0 36-37 33.157506845242494 38.5 33.0 40.5 2.0 41.0 38-39 33.17018166726824 39.0 33.0 41.0 2.0 41.0 40-41 33.10240538721038 39.0 33.0 41.0 2.0 41.0 42-43 33.01211631641299 39.0 33.0 41.0 2.0 41.0 44-45 32.91347282059436 39.0 33.0 41.0 2.0 41.0 46-47 32.7795618198912 39.0 33.0 41.0 2.0 41.0 48-49 32.65727448624679 38.5 32.5 41.0 2.0 41.0 50-51 32.54254726019343 38.0 32.0 41.0 2.0 41.0 52-53 32.3684330441815 38.0 32.0 40.0 2.0 41.0 54-55 32.20820612398826 38.0 32.0 40.0 2.0 41.0 56-57 32.04086333119595 38.0 31.0 40.0 2.0 41.0 58-59 31.90048241627604 38.0 31.0 40.0 2.0 41.0 60-61 31.827243257579546 38.0 31.0 40.0 2.0 41.0 62-63 31.641507414387284 38.0 31.0 40.0 2.0 41.0 64-65 31.216127589492324 37.0 30.5 40.0 2.0 41.0 66-67 31.03443033129291 37.0 30.0 40.0 2.0 41.0 68-69 30.669299204632686 36.0 30.0 39.5 2.0 41.0 70-71 30.406040270905585 36.0 29.0 39.0 2.0 41.0 72-73 30.13381303039529 35.5 29.0 39.0 2.0 41.0 74-75 29.806004773278087 35.0 29.0 38.5 2.0 40.0 76-77 28.679667728881892 34.0 27.5 36.5 2.0 39.0 78-79 29.110412864468962 35.0 29.0 37.0 2.0 39.0 80-81 28.941067697071016 35.0 29.0 36.5 2.0 39.0 82-83 28.634013705774713 35.0 29.0 36.0 2.0 39.0 84-85 28.327364452558292 34.5 29.0 36.0 2.0 37.0 86-87 27.990062863251584 34.0 28.0 35.5 2.0 37.0 88-89 27.715872361400884 34.0 27.5 35.0 2.0 36.5 90-91 27.440009505459173 34.0 27.0 35.0 2.0 36.0 92-93 27.19134657558778 34.0 26.0 35.0 2.0 36.0 94-95 26.932951591607655 34.0 26.0 35.0 2.0 36.0 96-97 26.669646470541526 34.0 25.5 35.0 2.0 35.5 98-99 26.336032953356384 34.0 25.0 35.0 2.0 35.0 100-101 25.429066353071853 33.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7900442827203307 1101 2 1.6816967298492465 1101 3 2.8329057551254806 1101 4 3.1685739557516897 1101 5 2.1585628913261132 1101 6 1.7492028773162929 1101 7 1.5492259701690116 1101 8 1.484361484508689 1101 9 1.5608403522580687 1101 10-11 1.6290253957951535 1101 12-13 1.5812214840022492 1101 14-15 1.7542684988674182 1101 16-17 1.8032685612576884 1101 18-19 1.790057774571693 1101 20-21 1.7363936289655442 1101 22-23 1.7375467638872877 1101 24-25 1.8466307384307754 1101 26-27 1.7594194570336725 1101 28-29 1.8583246103041944 1101 30-31 1.8694326425401826 1101 32-33 1.8239214641530452 1101 34-35 1.8694528897001703 1101 36-37 1.8260559118934339 1101 38-39 1.8057925891419302 1101 40-41 1.858160754785544 1101 42-43 1.8713241225281365 1101 44-45 1.8737064964247452 1101 46-47 1.8604261885805045 1101 48-49 1.8180783239833076 1101 50-51 1.8034884820859247 1101 52-53 1.8343935485166938 1101 54-55 1.8346622573577847 1101 56-57 1.785569063751268 1101 58-59 1.7648041726582804 1101 60-61 1.7075400816534767 1101 62-63 1.8522380974919201 1101 64-65 1.7913337700452985 1101 66-67 1.8382920490287482 1101 68-69 1.796523961517277 1101 70-71 1.8230699957113714 1101 72-73 1.8671389648017964 1101 74-75 1.7882126440280182 1101 76-77 1.684256449356731 1101 78-79 1.83818606623214 1101 80-81 1.8227089099713005 1101 82-83 1.8357376144726913 1101 84-85 1.882010967457564 1101 86-87 1.8084325043499554 1101 88-89 1.756279236869176 1101 90-91 1.8644832832000446 1101 92-93 1.9771326717120132 1101 94-95 1.9667621253709626 1101 96-97 1.9904676727219837 1101 98-99 2.031739002732511 1101 100-101 2.066257618770063 1102 1 1.7090860267146333 1102 2 1.5807674960042597 1102 3 2.7576714308000305 1102 4 3.1181324893294047 1102 5 2.112300081330247 1102 6 1.703254588243773 1102 7 1.4477077671762544 1102 8 1.4165519798218753 1102 9 1.4680927405429927 1102 10-11 1.546774697622709 1102 12-13 1.5885387520880627 1102 14-15 1.7735082111775142 1102 16-17 1.7291810100919918 1102 18-19 1.7220259355506755 1102 20-21 1.6867194033306419 1102 22-23 1.7538830229915874 1102 24-25 1.8125587763403317 1102 26-27 1.7957856995185182 1102 28-29 1.6931087468993624 1102 30-31 1.7189108251854677 1102 32-33 1.7666483187586266 1102 34-35 1.7693284492517947 1102 36-37 1.7831618489138066 1102 38-39 1.8201800861178299 1102 40-41 1.8301739498966185 1102 42-43 1.8024121580280905 1102 44-45 1.789808999864178 1102 46-47 1.8497875966651378 1102 48-49 1.8395523989012332 1102 50-51 1.8321670310797344 1102 52-53 1.8991753733857735 1102 54-55 1.8634290428578026 1102 56-57 1.881311963390658 1102 58-59 1.8021719418918565 1102 60-61 1.7683479398112354 1102 62-63 1.8601523081711235 1102 64-65 1.7954220316447973 1102 66-67 1.8074231793670812 1102 68-69 1.784193648000798 1102 70-71 1.6982330985184362 1102 72-73 1.7146437561317 1102 74-75 1.7717632187675108 1102 76-77 1.715287696611199 1102 78-79 1.77912482899524 1102 80-81 1.802434531533482 1102 82-83 1.7944089908170753 1102 84-85 1.810415485969532 1102 86-87 1.740644060811828 1102 88-89 1.6293919533545278 1102 90-91 1.7085542919573982 1102 92-93 1.793890523573424 1102 94-95 1.8402892103776054 1102 96-97 1.8529067844095017 1102 98-99 1.8281344190975268 1102 100-101 1.807816155676175 1103 1 1.7135834568840593 1103 2 1.5858241388879968 1103 3 2.758286922961716 1103 4 3.1005917588757015 1103 5 2.1551364579316825 1103 6 1.7153084229610798 1103 7 1.4709629968782068 1103 8 1.397010136263951 1103 9 1.5438971058707693 1103 10-11 1.5470177104933924 1103 12-13 1.5552565177435014 1103 14-15 1.7044307440565802 1103 16-17 1.7326604002654946 1103 18-19 1.7143993074759862 1103 20-21 1.7621587784866364 1103 22-23 1.796417108915243 1103 24-25 1.8081377255545164 1103 26-27 1.852325449157746 1103 28-29 1.8147745599367369 1103 30-31 1.8171495013684194 1103 32-33 1.7879638389953527 1103 34-35 1.8132412675435532 1103 36-37 1.7794813543255117 1103 38-39 1.8358070648869855 1103 40-41 1.7301352606600346 1103 42-43 1.7545070467537158 1103 44-45 1.840258661473257 1103 46-47 1.8653824069326177 1103 48-49 1.903363220436134 1103 50-51 1.879887751569811 1103 52-53 1.8621851983249442 1103 54-55 1.8704463268343972 1103 56-57 1.8972732390846332 1103 58-59 1.8857842497867807 1103 60-61 1.9114705307886837 1103 62-63 1.9527138385861775 1103 64-65 1.892226257279276 1103 66-67 1.9115792712903392 1103 68-69 1.8637782215748366 1103 70-71 1.8538429200675566 1103 72-73 1.8209855475530503 1103 74-75 1.8416103146569256 1103 76-77 1.7488194393113474 1103 78-79 1.7741411464578967 1103 80-81 1.7867645492065378 1103 82-83 1.788101177013239 1103 84-85 1.7829863761859954 1103 86-87 1.759427636854955 1103 88-89 1.5269186276624396 1103 90-91 1.5914861166810823 1103 92-93 1.5981009600458052 1103 94-95 1.627962090064269 1103 96-97 1.6355477822677322 1103 98-99 1.6436436288991239 1103 100-101 1.6734712553873798 1104 1 1.563032643575518 1104 2 1.437878712670738 1104 3 2.6086548851988027 1104 4 2.9879421102167214 1104 5 1.9535804776952972 1104 6 1.5855828510527346 1104 7 1.3539753974900037 1104 8 1.2780170129525104 1104 9 1.3487054928204145 1104 10-11 1.378850723362433 1104 12-13 1.4301250996317165 1104 14-15 1.5539253536966555 1104 16-17 1.5834857028305436 1104 18-19 1.5635476409026268 1104 20-21 1.5397801409526721 1104 22-23 1.5084291641033758 1104 24-25 1.5805846708619313 1104 26-27 1.5727066132362353 1104 28-29 1.5616952590946696 1104 30-31 1.5537887003041249 1104 32-33 1.5629104337319433 1104 34-35 1.562368021045792 1104 36-37 1.583320092345673 1104 38-39 1.6102862615083353 1104 40-41 1.5746397195708113 1104 42-43 1.5444236474903192 1104 44-45 1.5422773167555448 1104 46-47 1.5580224022137585 1104 48-49 1.5275197948132373 1104 50-51 1.5141493309832583 1104 52-53 1.5667518886485325 1104 54-55 1.6300727132347603 1104 56-57 1.5820908245078584 1104 58-59 1.5658136068156097 1104 60-61 1.569486964449041 1104 62-63 1.5881326905127366 1104 64-65 1.537185982924072 1104 66-67 1.6309758294519874 1104 68-69 1.5757737905038418 1104 70-71 1.551611561054255 1104 72-73 1.5185692114258487 1104 74-75 1.5599023702508248 1104 76-77 1.4430145510067192 1104 78-79 1.554559446623319 1104 80-81 1.5745357556608575 1104 82-83 1.5827464695920384 1104 84-85 1.5414229075373065 1104 86-87 1.5252129952348206 1104 88-89 1.5148282081158335 1104 90-91 1.5749821126745616 1104 92-93 1.590007726164231 1104 94-95 1.567507125998624 1104 96-97 1.4987908696805086 1104 98-99 1.5842677887933867 1104 100-101 1.620492346068044 1105 1 1.4173103550243553 1105 2 1.2864159098735115 1105 3 2.4537425875431076 1105 4 2.849604211912933 1105 5 1.760475641493926 1105 6 1.2785712917679604 1105 7 1.056828463060981 1105 8 0.9388588429746534 1105 9 0.9932704359428755 1105 10-11 1.0826612685795851 1105 12-13 1.15749883656364 1105 14-15 1.3340169207166355 1105 16-17 1.295057163718603 1105 18-19 1.284068069701 1105 20-21 1.295555975300914 1105 22-23 1.2966890409935914 1105 24-25 1.3496551551204732 1105 26-27 1.3331071248845987 1105 28-29 1.3059209521315367 1105 30-31 1.3083764095715082 1105 32-33 1.2803943286714485 1105 34-35 1.2514464312989944 1105 36-37 1.3155373177057612 1105 38-39 1.3373302089443158 1105 40-41 1.3331637944816634 1105 42-43 1.3630895476296132 1105 44-45 1.3094916584742435 1105 46-47 1.3830573991284467 1105 48-49 1.344061898096946 1105 50-51 1.3567608684135308 1105 52-53 1.3508582564078253 1105 54-55 1.2342817032085733 1105 56-57 1.324004733203779 1105 58-59 1.362328578300989 1105 60-61 1.3760595054928757 1105 62-63 1.3349258564994244 1105 64-65 1.3156002182958701 1105 66-67 1.4151552871733877 1105 68-69 1.398499005503158 1105 70-71 1.354262358446178 1105 72-73 1.31623917827498 1105 74-75 1.3829780257240145 1105 76-77 1.3468208401696948 1105 78-79 1.4611434539959554 1105 80-81 1.4742677112006142 1105 82-83 1.4280806710971206 1105 84-85 1.463931422610731 1105 86-87 1.4076409635189329 1105 88-89 1.4324820588839806 1105 90-91 1.4356854847082836 1105 92-93 1.4785881584833547 1105 94-95 1.5116018657153951 1105 96-97 1.4734127176932468 1105 98-99 1.5421005114457635 1105 100-101 1.5471405492510577 1106 1 1.2974104380698854 1106 2 1.1693181774944925 1106 3 2.386102910598762 1106 4 2.771071884118662 1106 5 1.766831546784097 1106 6 1.1794604590415432 1106 7 1.0397270071739442 1106 8 0.8508767565443307 1106 9 0.9562597618887594 1106 10-11 0.9951811747419228 1106 12-13 1.0309724340364355 1106 14-15 1.103936808810829 1106 16-17 1.1068078229277063 1106 18-19 1.0195872954458096 1106 20-21 0.9935780928267945 1106 22-23 0.9494864609442644 1106 24-25 0.9845507399317341 1106 26-27 1.0093847121704869 1106 28-29 1.0476641207544475 1106 30-31 0.9863565924191491 1106 32-33 0.8797304111247684 1106 34-35 0.9911994634197114 1106 36-37 1.032366847267994 1106 38-39 1.0153840774591743 1106 40-41 0.9810525181215439 1106 42-43 0.9711968400070248 1106 44-45 1.0030338014514228 1106 46-47 0.928407208971592 1106 48-49 1.0153576480319302 1106 50-51 1.063520097934159 1106 52-53 1.090817404017848 1106 54-55 1.1709683691417112 1106 56-57 1.2127176867223355 1106 58-59 1.180128739525074 1106 60-61 1.1769058302449054 1106 62-63 1.2089158508432476 1106 64-65 1.1525130061807545 1106 66-67 1.1912979475670262 1106 68-69 1.1640150033752796 1106 70-71 1.159445402742037 1106 72-73 1.175252569175612 1106 74-75 1.1918624906525288 1106 76-77 1.0806882488225469 1106 78-79 1.1685864608131915 1106 80-81 1.205604175191933 1106 82-83 1.1834997758450072 1106 84-85 1.2146466864194672 1106 86-87 1.214713301224993 1106 88-89 1.1756504821809415 1106 90-91 1.1668830022456191 1106 92-93 1.1414097116749957 1106 94-95 1.1673536553239714 1106 96-97 1.2023846785212875 1106 98-99 1.1997699528619243 1106 100-101 1.1863874328552448 1107 1 1.1571642337917822 1107 2 1.0782628044140878 1107 3 2.2008002260259154 1107 4 2.6223102384001677 1107 5 1.5818518359668232 1107 6 1.1313493082235198 1107 7 0.9706113660738822 1107 8 0.9000201513985147 1107 9 0.9366920346944383 1107 10-11 0.9366870926082811 1107 12-13 0.9252391722147806 1107 14-15 0.9723441655652181 1107 16-17 0.9017215746200833 1107 18-19 0.9224424738904133 1107 20-21 0.9255587477788154 1107 22-23 0.9362614466134644 1107 24-25 0.9081940524611412 1107 26-27 0.8679550076232303 1107 28-29 0.9161294062879861 1107 30-31 0.9448388786577979 1107 32-33 0.900580399281381 1107 34-35 0.9409603875453101 1107 36-37 0.9141885202135143 1107 38-39 0.906074793166681 1107 40-41 0.8998724105361049 1107 42-43 0.9765603993891006 1107 44-45 0.9664899790578616 1107 46-47 0.87866171205863 1107 48-49 0.9409733276941452 1107 50-51 0.9056905810756319 1107 52-53 0.9225324072038816 1107 54-55 0.8418496301179417 1107 56-57 0.8582355869164431 1107 58-59 0.9358904023168861 1107 60-61 0.9410641611104609 1107 62-63 1.0075401858915711 1107 64-65 0.8947358227636997 1107 66-67 0.9022626605557598 1107 68-69 0.9280384483739859 1107 70-71 0.9424806073303991 1107 72-73 0.9488360619089562 1107 74-75 0.9675847745283903 1107 76-77 0.8788939540181957 1107 78-79 0.956632901409531 1107 80-81 0.9856805282374843 1107 82-83 1.013760609796023 1107 84-85 0.9656958232259818 1107 86-87 0.9849471648319685 1107 88-89 0.9384952984965942 1107 90-91 0.8966276753220441 1107 92-93 0.8809744394606014 1107 94-95 0.8544030806106484 1107 96-97 0.864148146374685 1107 98-99 0.9529504482275009 1107 100-101 0.9228303130961386 1108 1 1.1245500014353986 1108 2 1.014474261992774 1108 3 2.131460391457381 1108 4 2.604576539631836 1108 5 1.4807000124167686 1108 6 1.0295131137568845 1108 7 0.7962305127348657 1108 8 0.7719295532515034 1108 9 0.7127943333743119 1108 10-11 0.7527677375335031 1108 12-13 0.8020301925001903 1108 14-15 0.7547315968336719 1108 16-17 0.8023022639987403 1108 18-19 0.7418989137220535 1108 20-21 0.7463949021891807 1108 22-23 0.747433889043144 1108 24-25 0.764991416242708 1108 26-27 0.873889848532869 1108 28-29 0.7971995799323892 1108 30-31 0.7627870137742789 1108 32-33 0.7199092656413839 1108 34-35 0.6573770279454436 1108 36-37 0.6858393075989042 1108 38-39 0.7329279573525511 1108 40-41 0.7310401981102075 1108 42-43 0.7073331978317015 1108 44-45 0.7653735016081242 1108 46-47 0.759926176202228 1108 48-49 0.7320371393835572 1108 50-51 0.7834917476546863 1108 52-53 0.7020636223032284 1108 54-55 0.7067001661277033 1108 56-57 0.7777828732501888 1108 58-59 0.6625427951538541 1108 60-61 0.7411686350726541 1108 62-63 0.7616488388197098 1108 64-65 0.7320103931446553 1108 66-67 0.7064792893398852 1108 68-69 0.6999007292612944 1108 70-71 0.5532361781585102 1108 72-73 0.5645899686150102 1108 74-75 0.5207160652778384 1108 76-77 0.4323455753508938 1108 78-79 0.5603633526056591 1108 80-81 0.553292849173296 1108 82-83 0.5866351859073013 1108 84-85 0.5658005221745235 1108 86-87 0.6476461963434481 1108 88-89 0.6572006820810614 1108 90-91 0.6320185569922501 1108 92-93 0.6831965743814905 1108 94-95 0.556404043514906 1108 96-97 0.38479079152296336 1108 98-99 0.4402527694308702 1108 100-101 0.47156112106964443 1109 1 0.7057074141856994 1109 2 0.6332351153221474 1109 3 0.09067762239104127 1109 4 1.4269887907422572 1109 5 0.7746080924488083 1109 6 0.6072806910363511 1109 7 0.5101046780868401 1109 8 0.4718565007721125 1109 9 0.4813305885540373 1109 10-11 0.4301244280962493 1109 12-13 0.4258821336154668 1109 14-15 0.3710751371456098 1109 16-17 0.4384099640064747 1109 18-19 0.4276946587627606 1109 20-21 0.3868667286194736 1109 22-23 0.42330300242087304 1109 24-25 0.4890643868760236 1109 26-27 0.5094219103503406 1109 28-29 0.45831888856947245 1109 30-31 0.3617129059180897 1109 32-33 0.4204513549129132 1109 34-35 0.38914004916251344 1109 36-37 0.40044759495184223 1109 38-39 0.44797238740423495 1109 40-41 0.411936163048054 1109 42-43 0.44163008909048784 1109 44-45 0.4268717508159554 1109 46-47 0.43624167527514857 1109 48-49 0.4799796363521196 1109 50-51 0.4100336617397602 1109 52-53 0.437458232015139 1109 54-55 0.43054486634924416 1109 56-57 0.43222064589813414 1109 58-59 0.40380966128515183 1109 60-61 0.39468128398398505 1109 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-0.2709734174497953 2311 90-91 -0.28139558400467024 2311 92-93 -0.25972847145182243 2311 94-95 -0.22709083692582155 2311 96-97 -0.19831099184347778 2311 98-99 -0.15820541235985885 2311 100-101 -0.11506369541515582 2312 1 0.42110070777550135 2312 2 0.5158129755985783 2312 3 1.7262773063360726 2312 4 1.7746207727973555 2312 5 0.7613063444215911 2312 6 0.44713875428102057 2312 7 0.3228763977590674 2312 8 0.26418114097679535 2312 9 0.21503400944256157 2312 10-11 0.19670771881018823 2312 12-13 0.1945033881015803 2312 14-15 -0.03509977704508316 2312 16-17 0.01110506380960885 2312 18-19 -0.4627982892840521 2312 20-21 -0.49246305336141916 2312 22-23 -0.16191837721236624 2312 24-25 0.10162642074978834 2312 26-27 0.1780083592561965 2312 28-29 0.13967543675121163 2312 30-31 -0.32292075254195396 2312 32-33 -0.44911316931150935 2312 34-35 -0.31460938638240776 2312 36-37 -0.16598871430903017 2312 38-39 -0.0654367393315951 2312 40-41 -0.5776262470240283 2312 42-43 -0.23594476694096755 2312 44-45 0.005845539117117937 2312 46-47 -0.179908433216184 2312 48-49 0.06860079095512361 2312 50-51 0.062413874775955946 2312 52-53 -0.2848497494640796 2312 54-55 0.010578317159733786 2312 56-57 0.05317036868378011 2312 58-59 0.13591929830904803 2312 60-61 0.1231416263065519 2312 62-63 0.07098307202839038 2312 64-65 0.17321698844678224 2312 66-67 -0.07591470907776099 2312 68-69 0.060249430121658065 2312 70-71 0.13735102966593615 2312 72-73 0.1753675038551421 2312 74-75 0.06652418613536781 2312 76-77 -0.2139082206303513 2312 78-79 -0.020969888146527893 2312 80-81 -0.015254165028007804 2312 82-83 -0.025045485845069493 2312 84-85 -0.03685820472479051 2312 86-87 -0.0036470842957392335 2312 88-89 1.0161924865315086E-4 2312 90-91 -0.03570938965426507 2312 92-93 -0.11756759995768107 2312 94-95 -0.12807523026360812 2312 96-97 -0.22808863352301856 2312 98-99 -0.2505273237287575 2312 100-101 -0.32166745237091376 2313 1 -1.4236674742002577 2313 2 -0.9143444353657841 2313 3 -3.8306085288133076 2313 4 -4.934587301910099 2313 5 -2.90442793876592 2313 6 -2.5902824201992942 2313 7 -1.6711443108980681 2313 8 -1.3064190802870357 2313 9 -1.2635116032159672 2313 10-11 -1.2097001303090167 2313 12-13 -1.1998107424586166 2313 14-15 -1.3027615719006178 2313 16-17 -1.524448673563029 2313 18-19 -1.4425762735605971 2313 20-21 -1.344268047436671 2313 22-23 -1.7942169795085476 2313 24-25 -1.5287946014598504 2313 26-27 -2.0197578044298297 2313 28-29 -2.08830213514776 2313 30-31 -1.77789067317552 2313 32-33 -1.8004169910696106 2313 34-35 -1.7214067712243768 2313 36-37 -1.9861770978746094 2313 38-39 -2.575240558331494 2313 40-41 -2.32299333371925 2313 42-43 -1.9175953900717388 2313 44-45 -1.7817723433892034 2313 46-47 -1.625700861867621 2313 48-49 -1.6479624458012658 2313 50-51 -1.6230502415370722 2313 52-53 -2.0249729258808813 2313 54-55 -1.7799263288262424 2313 56-57 -1.7680276970211821 2313 58-59 -1.7034753060231225 2313 60-61 -1.7593860425483854 2313 62-63 -1.7006891518551974 2313 64-65 -1.6062570804718774 2313 66-67 -1.8389656526018818 2313 68-69 -1.7763487653327203 2313 70-71 -1.7419161157786789 2313 72-73 -1.7577701042981673 2313 74-75 -1.6089761537753091 2313 76-77 -1.5276091325782133 2313 78-79 -1.6323834130086432 2313 80-81 -1.658540369851874 2313 82-83 -1.7163208060137762 2313 84-85 -1.6911599811213343 2313 86-87 -1.6687698523965722 2313 88-89 -1.592724430504937 2313 90-91 -1.6098780220597497 2313 92-93 -1.5261731727252084 2313 94-95 -1.523264702272634 2313 96-97 -1.5214330811860748 2313 98-99 -1.4637776754849234 2313 100-101 -1.432315692737756 2315 1 -3.9217715089296057 2315 2 -5.8005581825487695 2315 3 -5.080704421744482 2315 4 -5.342028986296807 2315 5 -4.118519250607147 2315 6 -3.446683138072835 2315 7 -3.0940283889084057 2315 8 -2.981165433562925 2315 9 -3.323611794375932 2315 10-11 -3.267540447355916 2315 12-13 -3.2903992484827143 2315 14-15 -3.5129346308349767 2315 16-17 -3.5168952451231235 2315 18-19 -3.512714893892337 2315 20-21 -3.5037261045762165 2315 22-23 -3.5516015241121046 2315 24-25 -3.613962567471212 2315 26-27 -3.5425531673937307 2315 28-29 -3.591732500868833 2315 30-31 -3.640107101324965 2315 32-33 -3.745585691529797 2315 34-35 -3.770409169591389 2315 36-37 -3.6300339763172005 2315 38-39 -3.6064557142904903 2315 40-41 -3.631816221621854 2315 42-43 -3.6220866404101884 2315 44-45 -3.624544137239269 2315 46-47 -3.695859071175672 2315 48-49 -3.705394762165877 2315 50-51 -3.6241270828302277 2315 52-53 -3.657453533513909 2315 54-55 -3.603531703884137 2315 56-57 -3.6380679324501344 2315 58-59 -3.645314047342616 2315 60-61 -3.6670168166620343 2315 62-63 -3.7186420205079997 2315 64-65 -3.5740368750149543 2315 66-67 -3.6515607460595874 2315 68-69 -3.6625651538804895 2315 70-71 -3.6862732256280637 2315 72-73 -3.7496037789685097 2315 74-75 -3.7491243421358753 2315 76-77 -3.6848492104322297 2315 78-79 -3.7673739551833556 2315 80-81 -3.793714881960824 2315 82-83 -3.772672487818653 2315 84-85 -3.7977958963038922 2315 86-87 -3.9198023780694733 2315 88-89 -3.8842889049123634 2315 90-91 -3.9307120718474273 2315 92-93 -3.8476826484316184 2315 94-95 -3.861429380505328 2315 96-97 -3.8058926351792053 2315 98-99 -3.6238667082880447 2315 100-101 -3.6742880413508807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1838931.0 3 469383.0 4 281072.0 5 265968.0 6 411706.0 7 373602.0 8 334669.0 9 456760.0 10 627341.0 11 605472.0 12 473341.0 13 369111.0 14 317321.0 15 292271.0 16 278003.0 17 267986.0 18 257900.0 19 253046.0 20 253609.0 21 264976.0 22 290685.0 23 335761.0 24 392274.0 25 459333.0 26 541000.0 27 638609.0 28 755908.0 29 894804.0 30 1066275.0 31 1293535.0 32 1560387.0 33 1952643.0 34 2531250.0 35 3453430.0 36 5088258.0 37 8430918.0 38 1.1209146E7 39 3836789.0 40 11084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.171577590347233 17.568849829936273 12.538927414198097 44.7206451655184 2 15.216130449685023 20.13651043559076 51.718372397372 12.928986717352217 3 20.703922239084942 23.936958874610788 34.577484018313555 20.781634867990718 4 22.21853786503279 30.979633583378668 30.155361906590088 16.646466644998455 5 21.119631708051433 33.61031476521229 30.931911419526877 14.338142107209398 6 16.472316410216497 36.124450104310064 32.27609866037462 15.127134825098821 7 13.416607848821824 19.139968985393345 51.123518410883115 16.31990475490171 8 14.502254737643245 21.584607234012314 43.54100743958784 20.372130588756608 9 15.59200622924322 21.997270874366293 43.212367529322115 19.198355367068373 10-11 18.971431421792364 33.360408621707485 31.526887082405995 16.141272874094156 12-13 17.73381499723045 26.246784142946368 38.1514649522238 17.86793590759939 14-15 18.43262727892465 27.79912696850379 36.6016189036334 17.16662684893816 16-17 19.515008624602594 27.52038057910291 34.75780207430334 18.20680872199115 18-19 19.625029441245225 28.198404389932136 34.04290131305846 18.133664855764184 20-21 19.60760552207261 28.219945110352647 33.367505505702354 18.804943861872385 22-23 19.10977195081969 29.609257141167532 33.40007523946486 17.880895668547918 24-25 18.85451397253891 29.61450631125233 32.80682305389861 18.724156662310147 26-27 19.214302244200137 29.58866301254354 32.374787584977256 18.822247158279065 28-29 19.245938553662086 29.55279201757186 32.282394858889965 18.918874569876085 30-31 18.96065916691575 29.96880023192277 31.926315897600233 19.144224703561246 32-33 19.205236905063803 29.91169304152159 31.644959653112924 19.23811040030168 34-35 19.613079564499373 30.392392389596683 30.66789827261979 19.326629773284154 36-37 19.97731394288308 30.147914471380037 30.81611348956664 19.058658096170245 38-39 19.547195887679063 30.24529131275576 31.071081261954003 19.13643153761117 40-41 19.77372522046487 30.088470935779384 30.859453241310987 19.278350602444768 42-43 19.684161649295667 29.998882173126685 31.275184557434997 19.04177162014265 44-45 19.67610411319727 29.76628376474978 31.38242461103742 19.175187511015533 46-47 19.53089466457695 29.693550809432356 31.506650786951507 19.26890373903918 48-49 19.656127187502776 29.6742617326821 31.157194474455245 19.512416605359885 50-51 19.440163678907734 29.84999506320319 31.752820864699554 18.957020393189524 52-53 20.06966119171822 29.843012022063032 30.97223859487817 19.115088191340572 54-55 19.651813615546217 30.2054954987993 30.684837132061592 19.45785375359289 56-57 19.854982139862816 30.07831527700749 30.556229361635403 19.510473221494294 58-59 19.510520417995206 30.492093345792398 30.596988899116447 19.400397337095953 60-61 19.484493547595243 30.6540966019227 30.041336463651696 19.820073386830366 62-63 19.833939348910167 30.458057163011482 30.34744526352306 19.36055822455529 64-65 20.23701731785702 30.38623700920276 30.107843226207127 19.268902446733087 66-67 19.78930112646749 30.86594020894926 29.900656849961344 19.444101814621913 68-69 19.91915189779502 30.559640523873032 30.300623288975814 19.220584289356133 70-71 19.991890488883378 30.73829539098253 29.879970794305443 19.38984332582865 72-73 19.790601786605087 30.809393618414582 29.95763774401065 19.442366850969677 74-75 19.576205342299502 30.33669589576497 30.783820906570654 19.303277855364875 76-77 19.360989701584693 30.79732124051831 30.30952023889354 19.532168819003463 78-79 19.74552416670145 30.877779385457472 30.145050560486432 19.231645887354645 80-81 19.550861710138193 31.05918406593301 30.0396331358994 19.350321088029393 82-83 19.91512163101523 31.11088118178821 29.63200679331 19.34199039388656 84-85 19.60560490491512 30.745428858492524 29.911813752250033 19.737152484342324 86-87 19.589424916934004 31.280840397560734 29.85720874461702 19.272525940888247 88-89 19.57848860241081 31.08706341895656 29.997892865131643 19.33655511350098 90-91 19.490015936036947 31.4802208114169 29.854975625421044 19.17478762712511 92-93 19.681792696408937 30.91370425693505 29.906276081350985 19.49822696530503 94-95 19.577210559858212 31.31468516793352 29.625461406188624 19.482642866019642 96-97 19.831867623635024 31.21289742290393 29.636030554488947 19.3192043989721 98-99 19.71836641587294 31.231721678668826 29.66038074644744 19.38953115901079 100-101 19.878195980011004 31.87803530765635 28.878810845634757 19.364957866697893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11706.0 1 15933.0 2 18161.5 3 18747.0 4 24923.0 5 33448.5 6 41514.5 7 49412.5 8 59399.0 9 69475.0 10 79467.5 11 89304.5 12 99058.5 13 108913.5 14 118808.0 15 129417.5 16 141095.0 17 154431.5 18 172240.0 19 194634.5 20 229406.0 21 302486.0 22 435947.5 23 506169.5 24 540554.0 25 676551.5 26 853464.5 27 1054774.0 28 1224734.0 29 1301620.5 30 1410769.0 31 1564523.0 32 1718484.0 33 1885951.0 34 2035481.0 35 2211616.0 36 2326667.0 37 2412251.5 38 2418545.0 39 2141573.5 40 1956284.0 41 1937254.0 42 1792558.0 43 1657036.5 44 1575818.0 45 1488456.5 46 1422742.0 47 1407713.5 48 1379733.0 49 1278802.5 50 1185557.0 51 1127804.5 52 1020754.0 53 903231.5 54 808994.5 55 696791.5 56 593714.0 57 496225.5 58 414885.5 59 342591.0 60 267897.5 61 202337.5 62 149832.0 63 107131.5 64 72907.0 65 50054.5 66 34982.0 67 25923.0 68 19277.0 69 14201.0 70 12505.0 71 14221.5 72 14347.5 73 17261.0 74 17846.5 75 11760.0 76 8602.5 77 7722.0 78 6554.0 79 4334.0 80 2975.0 81 2222.5 82 1704.5 83 1286.5 84 1043.5 85 861.5 86 645.0 87 453.0 88 317.0 89 236.0 90 159.0 91 93.5 92 53.0 93 33.0 94 14.5 95 2.5 96 3.0 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05260079165623101 2 0.005410356447794636 3 0.005777160274763764 4 0.0027061139479457085 5 0.021937114590469983 6 0.09889106033011558 7 0.10570313140239938 8 0.11321886695907296 9 0.12042768502787436 10-11 0.12713776217888362 12-13 0.13662413258146783 14-15 0.1398739022015285 16-17 0.14632010517089156 18-19 0.14651192860081164 20-21 0.1528804664741583 22-23 0.12405455143943646 24-25 0.13617124214204676 26-27 0.14363176997986526 28-29 0.15002463667847007 30-31 0.16050100312425158 32-33 0.16399218954879705 34-35 0.15952691438987696 36-37 0.11544214729804908 38-39 0.1747736020343539 40-41 0.1852022465536675 42-43 0.17676669425742594 44-45 0.19392600185681338 46-47 0.18764448631996705 48-49 0.19157546304725612 50-51 0.18785408850680657 52-53 0.18308283158406274 54-55 0.1819665127943327 56-57 0.1900268397471696 58-59 0.18996133906378226 60-61 0.19719448595784186 62-63 0.20383812670141535 64-65 0.37509434203786884 66-67 0.18173632467842862 68-69 0.34009826262805926 70-71 0.011107980178445196 72-73 0.012519052043418268 74-75 0.02543485108335417 76-77 0.029849597143661172 78-79 0.019513589305138246 80-81 0.022856931330037977 82-83 0.04118776543801046 84-85 0.02043808466494819 86-87 0.021002326266127743 88-89 0.025594859895628964 90-91 0.008816391983936538 92-93 0.013799122541616655 94-95 0.03132336251987642 96-97 0.03059911210642207 98-99 0.008344787063547658 100-101 0.01695999837708021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.3434557E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.325071531081235 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85863490515395 19.969839901283535 2 8.809197286753795 4.638054974104008 3 3.900415452194129 3.08036147339835 4 2.271005436821268 2.3913752228717713 5 1.531540202725375 2.0158952694736074 6 1.0288566401167265 1.6250834787780437 7 0.82811529523852 1.5260136068195544 8 0.6503765499435333 1.3696967359521073 9 0.4977244796294015 1.179236927611276 >10 3.863067090692798 20.169385211172703 >50 0.4347858054709379 7.952617099968634 >100 0.284869117571409 14.347815359129742 >500 0.02410249660123716 4.378996473784262 >1k 0.01491050787192587 7.590038359298125 >5k 0.0015037933558998682 2.705316424809554 >10k+ 8.94939859239875E-4 5.060273481544755 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 99512 0.1862315430068972 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 96803 0.18116179011271677 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 87650 0.16403242568287785 No Hit CCTGGATGGAAATTTGCATCAAACACTACAATAGTCAGTCGGATTTATTG 63381 0.11861425182209333 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 61396 0.11489942734998251 No Hit CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT 54878 0.10270132865516222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01719299366512948 1.8714480967812646E-6 0.0 1.1228688580687588E-5 0.0 2 0.017307151999033134 1.8714480967812646E-6 0.0 1.3100136677468852E-5 0.0 3 0.017511139841582292 1.8714480967812646E-6 0.0 1.4971584774250117E-5 0.0 4 0.019049470177136492 3.7428961935625292E-6 0.0 2.9943169548500234E-5 0.0 5 0.01944621717365412 3.7428961935625292E-6 0.0 2.9943169548500234E-5 0.0 6 0.020151753106140657 3.7428961935625292E-6 0.0 3.9300410032406554E-5 0.0 7 0.020501713900238753 3.7428961935625292E-6 0.0 4.304330622596908E-5 0.0 8 0.02124093589846735 7.4857923871250585E-6 0.0 5.614344290343794E-5 0.0 9 0.021851027978018047 9.357240483906323E-6 0.0 6.550068338734427E-5 0.0 10-11 0.023430530171701432 1.1228688580687588E-5 0.0 7.017930362929742E-5 0.0 12-13 0.026100150881759904 1.1228688580687588E-5 0.0 7.298647577446932E-5 0.0 14-15 0.028679942083172878 1.3100136677468852E-5 0.0 7.766509601642248E-5 0.0 16-17 0.03229745125425107 1.3100136677468852E-5 0.0 9.637957698423514E-5 0.0 18-19 0.035362883236778775 1.3100136677468852E-5 0.0 1.1041543771009462E-4 0.0 20-21 0.03849381590269384 1.3100136677468852E-5 0.0 1.1228688580687588E-4 0.0 22-23 0.04123268019233321 1.4035860725859485E-5 0.0 1.2725847058112598E-4 0.0 24-25 0.04351303969826118 1.4971584774250117E-5 0.0 1.3755143511342296E-4 0.0 26-27 0.04573538431318894 1.4971584774250117E-5 0.0 1.5439446798445433E-4 0.0 28-29 0.04781456314871292 1.4971584774250117E-5 0.0 1.6188026037157937E-4 0.0 30-31 0.04966729676452637 1.4971584774250117E-5 0.0 1.7217322490387636E-4 0.0 32-33 0.05157523809919487 1.684303287103138E-5 0.0 1.8340191348456393E-4 0.0 34-35 0.05381723291913883 1.684303287103138E-5 0.0 1.9556632611364216E-4 0.0 36-37 0.05552305785935495 1.684303287103138E-5 0.0 2.3393101209765805E-4 0.0 38-39 0.057320583756313354 1.7778756919422016E-5 0.0 2.72295698081674E-4 0.0 40-41 0.059090037931820036 1.8714480967812646E-5 0.0 3.031745916785649E-4 0.0 42-43 0.06119260986855379 1.8714480967812646E-5 0.0 3.1908190050120564E-4 9.357240483906323E-7 44-45 0.06343273324040097 1.9650205016203277E-5 0.0 3.480893460013152E-4 1.8714480967812646E-6 46-47 0.06591801631292649 2.1521653112984545E-5 0.0 4.0048989271119064E-4 1.8714480967812646E-6 48-49 0.06851932916745244 2.2457377161375175E-5 0.0 4.192043736790033E-4 1.8714480967812646E-6 50-51 0.0715501393601897 2.2457377161375175E-5 0.0 4.4634037108233157E-4 1.8714480967812646E-6 52-53 0.07498892523802528 2.432882525815644E-5 0.0 4.5663333561462855E-4 1.8714480967812646E-6 54-55 0.07869252102155538 2.432882525815644E-5 0.0 4.7347636848565997E-4 1.8714480967812646E-6 56-57 0.08270490574105444 2.6200273354937705E-5 0.0 4.95933745647035E-4 1.8714480967812646E-6 58-59 0.08739475467158828 2.6200273354937705E-5 0.0 5.436556721149573E-4 1.8714480967812646E-6 60-61 0.09259270176039824 2.6200273354937705E-5 0.0 5.857632542925358E-4 1.8714480967812646E-6 62-63 0.09854577815625945 2.6200273354937705E-5 0.0 6.269351124217236E-4 1.8714480967812646E-6 64-65 0.10535597778044646 2.7135997403328335E-5 9.357240483906323E-7 6.503282136314894E-4 1.8714480967812646E-6 66-67 0.11306072959489494 2.9007445500109603E-5 1.8714480967812646E-6 7.074073805833181E-4 1.8714480967812646E-6 68-69 0.12207643080113867 2.9943169548500234E-5 1.8714480967812646E-6 7.289290336963026E-4 1.8714480967812646E-6 70-71 0.13243396029277457 2.9943169548500234E-5 1.8714480967812646E-6 7.701008918254903E-4 1.8714480967812646E-6 72-73 0.144536615134659 3.368606574206276E-5 1.8714480967812646E-6 8.019155094707718E-4 1.8714480967812646E-6 74-75 0.1590225591277944 3.368606574206276E-5 1.8714480967812646E-6 8.27180058777319E-4 1.8714480967812646E-6 76-77 0.17593202840626151 3.4621789790453394E-5 1.8714480967812646E-6 8.655447447613348E-4 3.7428961935625292E-6 78-79 0.195541997288384 3.5557513838844024E-5 1.8714480967812646E-6 8.982950864550069E-4 3.7428961935625292E-6 80-81 0.21859917356477757 3.836468598401592E-5 1.8714480967812646E-6 9.26366807906726E-4 5.614344290343794E-6 82-83 0.24642386386772142 3.9300410032406554E-5 1.8714480967812646E-6 9.422741167293667E-4 7.4857923871250585E-6 84-85 0.2799976427239773 3.9300410032406554E-5 1.8714480967812646E-6 9.703458381810857E-4 9.357240483906323E-6 86-87 0.32127336622253644 3.9300410032406554E-5 1.8714480967812646E-6 0.0010190034886973986 1.1228688580687588E-5 88-89 0.37171076387888835 3.9300410032406554E-5 3.7428961935625292E-6 0.0010938614125686492 1.1228688580687588E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 39205 0.0 74.438965 1 GCGTGTT 55180 0.0 53.036804 2 CGTGTTT 83365 0.0 36.336105 3 GTTTTTT 674940 0.0 30.895624 1 TCGTCTA 33580 0.0 30.554014 44-45 ACTCGTC 34195 0.0 30.046295 42-43 CTAACGA 34520 0.0 28.959223 94-95 CCTTTAG 42875 0.0 28.09785 5 CGCGCGT 1820 0.0 27.916542 1 CCCGAAA 37265 0.0 27.141117 1 TTTAGGC 47665 0.0 25.457663 7 TTAGGCA 47755 0.0 25.111448 8 TAGGCAT 48395 0.0 24.750141 9 GCCTAAC 42055 0.0 23.759865 92-93 GTCTATG 44130 0.0 23.415983 46-47 CTTTAGG 53920 0.0 22.333681 6 CCGAAAC 45920 0.0 22.026073 2 CGAGCTA 48200 0.0 21.78339 12-13 TAACGAG 27465 0.0 21.72657 94-95 TCGACTC 3725 0.0 21.40663 3 >>END_MODULE