FastQCFastQC Report
Fri 27 May 2016
SRR1233028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96514469
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT2234700.23154041286804367No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT2017350.20902047339658472No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1842810.19093613829031167No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG1721950.17841366355131685No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC1451820.15042511397954228No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT1395680.14460836954923306No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1353150.1402017763782133No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1349910.13986607541714807No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1288070.13345874596274265No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1270980.1316880270045313No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1269230.131506707040993No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1232830.12773525179939602No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1173530.12159109532064048No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1134990.11759791166648806No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1092780.11322447414594387No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT1058390.1096612778338966No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT1043570.1081257567712464No Hit
ATTTAATACGACTCACTATAGAGAATTTAATACGACTCACTATAGAGAAT1041700.10793200343877972No Hit
CGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATA995540.10314930085767761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT576950.056.024681
GCGTGTT696500.045.5653042
GTTTTTT11546850.044.5029261
CGTCGTA138950.034.2238461
CGTGTTT1004850.031.956183
CTCGTAT140850.030.6928921
TCGTCTA343900.030.13263144-45
CCGAAAC467550.029.8522471
CTAACGA338700.028.30592394-95
CGCCGTA273850.028.28329846-47
ACTCGTC377850.027.53116242-43
GTCGCCG282800.027.50526844-45
CGAAACC503200.027.2816772
TAACGAG327450.025.31657694-95
CCGTATC306150.025.24483148-49
CGCGCGT30300.025.0859531
CGAGCTA423050.024.7853812-13
CCTAACG325650.024.71523392-93
CCCGAAA325700.024.5336131
CTTTATT3220350.024.3584611
Produced by FastQC (version 0.11.5)