FastQCFastQC Report
Fri 27 May 2016
SRR1233028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96514469
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT2213300.2293231287424894No Hit
ATTTAATACGACTCACTATAGGGAATTTAATACGACTCACTATAGGGAAT1897150.19656638218669575No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT1752250.18155308920572313No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG1621380.1679934642752891No Hit
ATACGACTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACT1436440.14883157052855983No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC1399860.1450414652335703No Hit
ATTAAATTCCCTATAGTGAGTCGTATTAAATTCCCTATAGTGAGTCGTAT1353160.14020281249229066No Hit
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA1238170.1282885367167072No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT1227650.12719854470732259No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC1194980.12381356001658154No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1183970.1226727984174062No Hit
CTCACTATAGATTGTAATACGACTCACTATAGATTGTAATACGACTCACT1177500.12200243260935312No Hit
ATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGT1058870.10971101130960996No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1045320.10830707673478472No Hit
GTCGTATTATCCCTATAGTGAGTCGTATTATCCCTATAGTGAGTCGTATT1042220.10798588137080253No Hit
CTCACTATAGGGATAATACGACTCACTATAGGGATAATACGACTCACTAT1034750.10721190415501328No Hit
ATTTAATACGACTCACTATAGGAATTTAATACGACTCACTATAGGAATTT981300.10167387441151439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT594200.057.77551
GCGTGTT730550.046.2759132
GTTTTTT11391250.044.267541
CGTGTTT1043550.032.5918773
CGTCGTA142050.031.5026951
CCGAAAC436250.030.5989341
TCGTCTA328900.029.65796944-45
CGAAACC466900.028.3540152
CTAACGA323450.027.3732794-95
ACTCGTC360850.027.19609542-43
TGCCGTC399300.026.51923448-49
TATGCCG416850.025.96008546-47
CGAGCTA391400.025.03081912-13
CCTAACG306050.024.67882392-93
CCCGAAA285000.024.4784891
CCGTCTT433850.024.3694850-51
CCAAACG548300.024.1192387
TAACGAG322150.024.08794494-95
CTTTATT3179850.022.8665261
CGCGTTT63450.022.4847971
Produced by FastQC (version 0.11.5)