##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233027_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85591324 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.98064085327153 31.0 28.0 33.0 16.0 34.0 2 28.363890550402047 31.0 28.0 33.0 16.0 34.0 3 28.142536841701386 31.0 27.0 33.0 16.0 34.0 4 31.18288265992941 35.0 30.0 37.0 16.0 37.0 5 30.96930623482352 35.0 30.0 37.0 10.0 37.0 6 30.98809945970692 35.0 30.0 37.0 15.0 37.0 7 31.442364520497428 35.0 32.0 37.0 17.0 37.0 8 31.36019060763682 35.0 32.0 37.0 17.0 37.0 9 32.320290652356306 37.0 32.0 39.0 16.0 39.0 10-11 31.89864227944412 37.0 30.0 39.0 11.0 39.0 12-13 31.773794123105283 37.0 30.0 39.0 11.0 39.0 14-15 32.29983078658767 37.0 30.5 40.0 10.5 41.0 16-17 31.77231040379747 37.0 30.0 39.5 10.0 41.0 18-19 31.417601876330362 36.5 29.0 39.5 10.0 41.0 20-21 31.013272630296033 36.0 27.0 39.0 9.5 41.0 22-23 30.5806074924136 36.0 27.0 39.0 5.0 40.0 24-25 30.482410594559795 36.0 27.0 39.0 2.0 41.0 26-27 30.200181656262263 36.0 27.0 39.0 2.0 41.0 28-29 29.865956519144394 36.0 25.5 39.0 2.0 41.0 30-31 29.537564870476828 36.0 25.0 39.0 2.0 41.0 32-33 29.2823491315545 36.0 24.5 39.0 2.0 41.0 34-35 28.971704550335033 36.0 24.0 39.0 2.0 41.0 36-37 28.784236472378907 35.5 24.0 39.0 2.0 41.0 38-39 28.601327664939497 35.0 23.5 39.0 2.0 40.5 40-41 28.449002973712616 35.0 23.0 39.0 2.0 40.0 42-43 28.233416122877127 35.0 21.5 39.0 2.0 40.0 44-45 28.002151567371477 35.0 19.0 39.0 2.0 40.0 46-47 27.798585958315122 35.0 18.5 39.0 2.0 40.0 48-49 27.544468969775487 34.5 18.0 39.0 2.0 40.0 50-51 27.039567760395904 33.5 15.0 38.5 2.0 40.0 52-53 27.08491024160346 34.0 15.0 38.0 2.0 40.0 54-55 27.21720072352193 34.0 15.0 39.0 2.0 40.0 56-57 27.054822501635797 34.0 13.5 39.0 2.0 40.0 58-59 26.746667536069427 34.0 12.0 38.5 2.0 40.0 60-61 26.476618763369054 34.0 9.0 38.0 2.0 40.0 62-63 26.114078939823386 33.0 8.0 38.0 2.0 40.0 64-65 25.753931239572832 33.0 8.0 38.0 2.0 40.0 66-67 25.4441776598759 32.5 7.0 38.0 2.0 40.0 68-69 25.137467612955724 32.0 4.0 37.0 2.0 40.0 70-71 24.937891397730922 32.0 2.0 37.0 2.0 39.0 72-73 24.683689038388984 32.0 2.0 36.5 2.0 39.0 74-75 24.301550411815107 31.0 2.0 36.0 2.0 39.0 76-77 23.805445321771167 31.0 2.0 35.5 2.0 38.5 78-79 23.422233309534974 31.0 2.0 35.0 2.0 38.0 80-81 23.058916771751306 30.0 2.0 35.0 2.0 37.0 82-83 22.69243430560789 30.0 2.0 35.0 2.0 37.0 84-85 22.333804621365594 30.0 2.0 34.5 2.0 36.0 86-87 21.89383802498487 29.5 2.0 34.0 2.0 36.0 88-89 21.5084191827667 29.0 2.0 34.0 2.0 35.0 90-91 21.1045463439729 29.0 2.0 34.0 2.0 35.0 92-93 20.66501144438425 29.0 2.0 34.0 2.0 35.0 94-95 20.1548033478253 28.5 2.0 34.0 2.0 35.0 96-97 19.544857928590986 27.0 2.0 34.0 2.0 35.0 98-99 18.72545827775722 25.5 2.0 34.0 2.0 35.0 100-101 16.49327098854085 13.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5696459413806423 1101 2 1.4569694876170871 1101 3 1.5813639276819558 1101 4 1.76560644165448 1101 5 1.8564927902901758 1101 6 1.9024479353865758 1101 7 1.793204701953936 1101 8 1.8653808870898239 1101 9 2.050829381064311 1101 10-11 2.0856386439312544 1101 12-13 2.040550112645189 1101 14-15 2.269924137647969 1101 16-17 2.6118554812394024 1101 18-19 2.5086267731728675 1101 20-21 2.5295436261795174 1101 22-23 2.505808962728157 1101 24-25 2.4611917292655043 1101 26-27 2.4577528304298504 1101 28-29 2.4365570742270783 1101 30-31 2.38469824101767 1101 32-33 2.503065968848901 1101 34-35 2.490596769761126 1101 36-37 2.5612609299756777 1101 38-39 2.5517840634081708 1101 40-41 2.5739892910116 1101 42-43 2.573270224761032 1101 44-45 2.652058070083065 1101 46-47 2.455939781874804 1101 48-49 2.5893187146896643 1101 50-51 2.5599923278719494 1101 52-53 2.469296808282966 1101 54-55 2.50224554205753 1101 56-57 2.628437388703041 1101 58-59 2.6790630244685048 1101 60-61 2.6823981206626826 1101 62-63 2.6883236325977258 1101 64-65 2.676170660411202 1101 66-67 2.744504708171558 1101 68-69 2.767995644352375 1101 70-71 2.783930506005607 1101 72-73 2.7227509130683956 1101 74-75 2.675850768195019 1101 76-77 2.8827313732822937 1101 78-79 2.887726637919261 1101 80-81 2.8029050352458107 1101 82-83 2.7830700246359363 1101 84-85 2.8057881554514204 1101 86-87 2.7779066266771295 1101 88-89 2.723474281859904 1101 90-91 2.7814715843424125 1101 92-93 2.844663125539295 1101 94-95 2.97209540008733 1101 96-97 2.977792028656488 1101 98-99 3.0427858300875172 1101 100-101 3.109429187483922 1102 1 1.3971148500690553 1102 2 1.321717970515607 1102 3 1.3905722453379994 1102 4 1.6056704924921554 1102 5 1.6558195989504156 1102 6 1.6854953323160728 1102 7 1.6276143054598613 1102 8 1.6230792560733995 1102 9 1.8213065140581293 1102 10-11 1.913137106960356 1102 12-13 1.9085421594670073 1102 14-15 1.9487452940526921 1102 16-17 2.2878805455138007 1102 18-19 2.191121149401763 1102 20-21 2.26697542551846 1102 22-23 2.157266603642743 1102 24-25 2.1081305702604034 1102 26-27 2.1009114854230937 1102 28-29 2.1670673642080196 1102 30-31 2.1721332863752245 1102 32-33 2.2040802074353536 1102 34-35 2.2600268777299277 1102 36-37 2.2552241335266103 1102 38-39 2.253814051148048 1102 40-41 2.259755974363351 1102 42-43 2.353472039580815 1102 44-45 2.4084737472484967 1102 46-47 2.3248705262991614 1102 48-49 2.4132008951103607 1102 50-51 2.388524465433825 1102 52-53 2.3222921621507027 1102 54-55 2.465980553370162 1102 56-57 2.4520815311278206 1102 58-59 2.4529937543003264 1102 60-61 2.4433961203354784 1102 62-63 2.4388794929441993 1102 64-65 2.456852188479843 1102 66-67 2.414913857746697 1102 68-69 2.512845653559012 1102 70-71 2.5640107514022183 1102 72-73 2.5664767281111835 1102 74-75 2.517437318715128 1102 76-77 2.611893730106889 1102 78-79 2.645588700207586 1102 80-81 2.6088836358079384 1102 82-83 2.576045943626678 1102 84-85 2.533437008244036 1102 86-87 2.4863807138911973 1102 88-89 2.5009261650120465 1102 90-91 2.600824329138753 1102 92-93 2.617025410161407 1102 94-95 2.719127122722025 1102 96-97 2.8022377364423185 1102 98-99 2.8630875728723026 1102 100-101 2.914338322232364 1103 1 1.377626401366932 1103 2 1.3157012041369107 1103 3 1.358991891160347 1103 4 1.6136522414531136 1103 5 1.711171535283718 1103 6 1.609428377894595 1103 7 1.4484981509828536 1103 8 1.4411268079131112 1103 9 1.7431997835840534 1103 10-11 1.8278826312473697 1103 12-13 1.8552419413218324 1103 14-15 2.017633716708424 1103 16-17 2.2557249398216896 1103 18-19 2.2039455143267936 1103 20-21 2.22440497338458 1103 22-23 2.233255889890547 1103 24-25 2.0808861050361322 1103 26-27 2.0522455899759358 1103 28-29 2.093196750287664 1103 30-31 2.0956933991877094 1103 32-33 2.119647636030514 1103 34-35 2.19206283374076 1103 36-37 2.206032615959824 1103 38-39 2.264346133786262 1103 40-41 2.271170511910526 1103 42-43 2.3264455408704734 1103 44-45 2.377999497178955 1103 46-47 2.3781330043347566 1103 48-49 2.4255258365231747 1103 50-51 2.354185289811685 1103 52-53 2.291101289288946 1103 54-55 2.357083945898573 1103 56-57 2.4000835456372513 1103 58-59 2.464409989005997 1103 60-61 2.501501352240467 1103 62-63 2.4913392617493137 1103 64-65 2.4369447648758538 1103 66-67 2.4343520615786467 1103 68-69 2.582832724420623 1103 70-71 2.6475893703399294 1103 72-73 2.587977189109182 1103 74-75 2.6166915312200842 1103 76-77 2.777811741378148 1103 78-79 2.7284739466718015 1103 80-81 2.6607054866053055 1103 82-83 2.6166743412878084 1103 84-85 2.6164474351118585 1103 86-87 2.609001762289857 1103 88-89 2.68372752489951 1103 90-91 2.7607879172221885 1103 92-93 2.779896802713992 1103 94-95 2.8379874156154514 1103 96-97 2.891598976879795 1103 98-99 3.0511834199875807 1103 100-101 3.0564927482441213 1104 1 1.2576558664927653 1104 2 1.2471661846667672 1104 3 1.306615586872951 1104 4 1.510158225196765 1104 5 1.5495814741484182 1104 6 1.4854087018983293 1104 7 1.4452780902432032 1104 8 1.4570935414481276 1104 9 1.6135990610161386 1104 10-11 1.6676684414533298 1104 12-13 1.597355335340911 1104 14-15 1.8205804722027565 1104 16-17 2.042414834806621 1104 18-19 1.9699885982181264 1104 20-21 1.9282924522791234 1104 22-23 1.972351172505487 1104 24-25 1.9124089509757631 1104 26-27 1.909180342036418 1104 28-29 1.9130354859670042 1104 30-31 1.8984032777981135 1104 32-33 1.9975295922884584 1104 34-35 2.003319298952313 1104 36-37 2.016224924494061 1104 38-39 2.0635058744203434 1104 40-41 2.0326990996829934 1104 42-43 2.1301080464712356 1104 44-45 2.163019102570999 1104 46-47 2.162531477124084 1104 48-49 2.282142826193766 1104 50-51 2.2374946262231568 1104 52-53 2.184055138939417 1104 54-55 2.292986846923899 1104 56-57 2.2911752740245603 1104 58-59 2.3481281006079264 1104 60-61 2.320420517525612 1104 62-63 2.2718242215997435 1104 64-65 2.3006663817707533 1104 66-67 2.437471151029417 1104 68-69 2.5162902820666204 1104 70-71 2.4804379043815743 1104 72-73 2.4611519816282694 1104 74-75 2.4474859569171237 1104 76-77 2.598826405823967 1104 78-79 2.5962057035557073 1104 80-81 2.605297878607832 1104 82-83 2.5288644373078633 1104 84-85 2.5604350818426482 1104 86-87 2.5977832952368374 1104 88-89 2.6509596383739407 1104 90-91 2.692056295175906 1104 92-93 2.7061803363969084 1104 94-95 2.9226444191217062 1104 96-97 2.99697541300851 1104 98-99 3.0864335040027235 1104 100-101 3.1262160893770066 1105 1 0.08293306648719323 1105 2 0.011721107611169401 1105 3 0.01602227721508953 1105 4 0.07361363296993062 1105 5 0.11586299447196069 1105 6 0.11136304793696539 1105 7 0.12162218213028098 1105 8 -0.4746149222061433 1105 9 -0.18087370153380533 1105 10-11 -0.0014167242263027902 1105 12-13 0.0576085878887973 1105 14-15 0.2085620628920637 1105 16-17 0.3304590448761502 1105 18-19 0.16403790967381937 1105 20-21 0.31389450737825797 1105 22-23 0.38523298823328034 1105 24-25 0.207343431562645 1105 26-27 0.17971311832323167 1105 28-29 0.2106580055615055 1105 30-31 0.12015478839230909 1105 32-33 0.1647093157619679 1105 34-35 0.2851791836408424 1105 36-37 0.2571682621583058 1105 38-39 0.2972665265073253 1105 40-41 0.330119446658113 1105 42-43 0.3904260869372749 1105 44-45 0.37594014822973065 1105 46-47 0.408297644290915 1105 48-49 0.44622560111430687 1105 50-51 0.3895166778750827 1105 52-53 0.3239953080649727 1105 54-55 0.3660514205302512 1105 56-57 0.3200660910595694 1105 58-59 0.3048594818952779 1105 60-61 0.41406382947505094 1105 62-63 0.49455458670858476 1105 64-65 0.43563404713029996 1105 66-67 0.4652671276074827 1105 68-69 0.5444476319058893 1105 70-71 0.602932392360799 1105 72-73 0.5578777149032916 1105 74-75 0.5540704035947748 1105 76-77 0.7185784569469327 1105 78-79 0.6873810346588947 1105 80-81 0.7495878655996258 1105 82-83 0.7237022409598062 1105 84-85 0.7128661535229881 1105 86-87 0.7093107909048584 1105 88-89 0.7462652917543693 1105 90-91 0.898189458898468 1105 92-93 0.9585611661083142 1105 94-95 1.0771691281778537 1105 96-97 1.184067267979593 1105 98-99 1.361570331835125 1105 100-101 1.6557240350829794 1106 1 -0.4171240788307884 1106 2 -0.43326554963842057 1106 3 -0.5655620143434454 1106 4 -0.5152746310587268 1106 5 -0.4982474119287623 1106 6 -0.48233735840647185 1106 7 -0.5921879041685649 1106 8 -0.7370425793714759 1106 9 -0.6777084924291721 1106 10-11 -0.5514662293773576 1106 12-13 -0.5888717029614874 1106 14-15 -0.6211583787978299 1106 16-17 -0.5717826638967196 1106 18-19 -0.6660049768994121 1106 20-21 -0.5579681758021451 1106 22-23 -0.6201527542848488 1106 24-25 -0.6805302107508417 1106 26-27 -0.7585351126411943 1106 28-29 -0.6430930443914811 1106 30-31 -0.6665699014289466 1106 32-33 -0.682869130057675 1106 34-35 -0.64053696057114 1106 36-37 -0.6095831771614542 1106 38-39 -0.5925596677176159 1106 40-41 -0.593164314435235 1106 42-43 -0.5982574784130676 1106 44-45 -0.5465474875878513 1106 46-47 -0.5263676756096274 1106 48-49 -0.5562052496024172 1106 50-51 -0.548594340718612 1106 52-53 -0.6151169432431942 1106 54-55 -0.6615547293491026 1106 56-57 -0.6722980627992712 1106 58-59 -0.6360387785559141 1106 60-61 -0.5679035031320154 1106 62-63 -0.5408887990974698 1106 64-65 -0.584296363439595 1106 66-67 -0.5553191194374669 1106 68-69 -0.4946463586263192 1106 70-71 -0.47597148848417703 1106 72-73 -0.4784583984024522 1106 74-75 -0.48711846048422913 1106 76-77 -0.30012025394766084 1106 78-79 -0.3198240906679253 1106 80-81 -0.267645682651132 1106 82-83 -0.28463636884321986 1106 84-85 -0.3151821370651433 1106 86-87 -0.3421711989134124 1106 88-89 -0.30480835289800723 1106 90-91 -0.1813496149610323 1106 92-93 -0.08131163447772138 1106 94-95 0.06404062803471078 1106 96-97 0.06650334132188718 1106 98-99 0.11903744130219351 1106 100-101 0.364640329840892 1107 1 -1.0102618700086623 1107 2 -0.9476806406983265 1107 3 -0.8745082679516152 1107 4 -0.901897008811197 1107 5 -1.0386971621453647 1107 6 -1.1398470483551932 1107 7 -1.004779526679318 1107 8 -1.1107265387520755 1107 9 -1.1193828692406456 1107 10-11 -1.2973986248704819 1107 12-13 -1.4366901561648682 1107 14-15 -1.4945288617738903 1107 16-17 -1.2088260863057556 1107 18-19 -1.4394112577323526 1107 20-21 -1.3392316363001946 1107 22-23 -1.409705180625366 1107 24-25 -1.4838624839206425 1107 26-27 -1.4799984766755578 1107 28-29 -1.4429244433994413 1107 30-31 -1.3959693738090841 1107 32-33 -1.305761309646634 1107 34-35 -1.3502430245576917 1107 36-37 -1.3774886546585918 1107 38-39 -1.3489716701750005 1107 40-41 -1.3457017482988576 1107 42-43 -1.4910316892666913 1107 44-45 -1.5863454428950945 1107 46-47 -1.4417910233083084 1107 48-49 -1.331466839182422 1107 50-51 -1.403806369924716 1107 52-53 -1.4008943755271588 1107 54-55 -1.2148735392434915 1107 56-57 -1.3242979735832883 1107 58-59 -1.2857378540706819 1107 60-61 -1.4064619951351283 1107 62-63 -1.491190514858605 1107 64-65 -1.3612506336579528 1107 66-67 -1.26480993296461 1107 68-69 -1.261784401377561 1107 70-71 -1.3358714410788224 1107 72-73 -1.2577792834810708 1107 74-75 -1.2164374557699311 1107 76-77 -1.0655553571055485 1107 78-79 -1.1373353442001353 1107 80-81 -1.1805192136600802 1107 82-83 -1.0546629610514842 1107 84-85 -1.055070102047985 1107 86-87 -1.0017234274445492 1107 88-89 -0.9437367190081005 1107 90-91 -0.8200426212065821 1107 92-93 -0.9196680125059764 1107 94-95 -0.9679884009371662 1107 96-97 -0.8875155858952759 1107 98-99 -0.802720988051302 1107 100-101 -0.4361713897662973 1108 1 -1.0706486023872408 1108 2 -0.748184278499771 1108 3 -1.3411037975268592 1108 4 -1.1103681775991987 1108 5 -1.2096632576866995 1108 6 -2.585572824162494 1108 7 -1.6654607094677978 1108 8 -1.1541228828702366 1108 9 -0.5725914331187276 1108 10-11 -0.6258592725948091 1108 12-13 -0.8284443651927482 1108 14-15 -1.943066758377178 1108 16-17 -1.3828331901150435 1108 18-19 -2.915418708226831 1108 20-21 -1.367157896986516 1108 22-23 -1.6185968431752293 1108 24-25 -1.4400146564450829 1108 26-27 -1.6150749855315354 1108 28-29 -1.5330882265765347 1108 30-31 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38-39 -3.6661765974382554 2307 40-41 -3.767675613614152 2307 42-43 -3.605559329505496 2307 44-45 -3.554632432013932 2307 46-47 -3.55498228988165 2307 48-49 -3.2949361147362985 2307 50-51 -3.6307727283556552 2307 52-53 -3.5112199101836303 2307 54-55 -3.43069594027315 2307 56-57 -3.3996480268975624 2307 58-59 -3.393062800089176 2307 60-61 -3.5235825273392507 2307 62-63 -3.4106250033016146 2307 64-65 -3.5194008209273306 2307 66-67 -3.5607605616755826 2307 68-69 -3.5547165207952673 2307 70-71 -3.4516522843638597 2307 72-73 -3.313587614491407 2307 74-75 -3.309888995554399 2307 76-77 -3.2731654946475537 2307 78-79 -3.434699321121066 2307 80-81 -3.3730073320907508 2307 82-83 -3.347669881750292 2307 84-85 -3.1340964027563167 2307 86-87 -3.13157246909849 2307 88-89 -3.1148254637220347 2307 90-91 -2.9599249026682983 2307 92-93 -2.956822292021247 2307 94-95 -2.845700297261093 2307 96-97 -2.7514597135031487 2307 98-99 -2.7072363887827606 2307 100-101 -2.2526451835615937 2308 1 -3.6063856979098574 2308 2 -4.046618132911373 2308 3 -3.6168039977374065 2308 4 -5.2346496161530744 2308 5 -6.109564135841936 2308 6 -5.657036927595929 2308 7 -5.007658930951607 2308 8 -4.741010387611997 2308 9 -4.877216726165738 2308 10-11 -5.510669668528877 2308 12-13 -4.971342784843738 2308 14-15 -7.204420024327444 2308 16-17 -7.179476648272402 2308 18-19 -5.916837131039021 2308 20-21 -6.625954600973891 2308 22-23 -5.1893922782301445 2308 24-25 -6.1964193876319165 2308 26-27 -5.611819852323357 2308 28-29 -5.4796485671233945 2308 30-31 -6.040753226174392 2308 32-33 -6.331885234969924 2308 34-35 -6.432769453277768 2308 36-37 -5.113866308621937 2308 38-39 -5.521889346780576 2308 40-41 -4.956683547585996 2308 42-43 -5.49274131819973 2308 44-45 -5.760055915219581 2308 46-47 -5.253454361230261 2308 48-49 -4.814698774172253 2308 50-51 -5.010916875315644 2308 52-53 -4.677232733271065 2308 54-55 -5.0553878882816505 2308 56-57 -5.370751858437654 2308 58-59 -5.054648196025919 2308 60-61 -4.672199437994756 2308 62-63 -4.787276546886918 2308 64-65 -4.52669401752733 2308 66-67 -4.834989052001035 2308 68-69 -5.044882655772657 2308 70-71 -4.844698964747465 2308 72-73 -4.539179854352497 2308 74-75 -4.568494922730483 2308 76-77 -4.4167262919709955 2308 78-79 -4.674819741304642 2308 80-81 -4.388095930297364 2308 82-83 -4.331365698223276 2308 84-85 -4.164863590962241 2308 86-87 -4.1517590190603 2308 88-89 -4.111876852233454 2308 90-91 -4.08062599812785 2308 92-93 -4.010960783303137 2308 94-95 -3.9300422999243985 2308 96-97 -3.949008462439572 2308 98-99 -3.9281260508777223 2308 100-101 -3.2561989738275052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3562196.0 3 955665.0 4 875287.0 5 875775.0 6 998653.0 7 1263454.0 8 1582763.0 9 1842685.0 10 1767587.0 11 1530211.0 12 1259612.0 13 1050710.0 14 927579.0 15 864269.0 16 839389.0 17 839371.0 18 856574.0 19 900563.0 20 963445.0 21 1042999.0 22 1143734.0 23 1262843.0 24 1397786.0 25 1552821.0 26 1734983.0 27 1955527.0 28 2215230.0 29 2525328.0 30 2896772.0 31 3350795.0 32 3915069.0 33 4625965.0 34 5521606.0 35 6602424.0 36 7680566.0 37 7671719.0 38 4269219.0 39 470076.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.102407918632807 15.343228524224417 9.88345951051985 49.67090404662293 2 13.090891140701691 18.361333001520034 56.25817177881138 12.289604078966892 3 17.630028885117195 21.76639916514382 40.638595577865324 19.964976371873654 4 19.927156630535823 29.023919225078416 33.79801091507903 17.25091322930674 5 20.389140214291714 32.843031192908285 32.700417896076026 14.067410696723975 6 15.755806453674465 34.43466098312759 34.40555053797484 15.40398202522311 7 13.426018798646878 17.05249008850855 53.24232016117012 16.27917095167446 8 14.007525588149358 21.129680832549298 44.39785293775116 20.464940641550186 9 15.843596510551874 21.802275549754334 43.54200493894319 18.812123000750603 10-11 19.04620791270722 33.538932326763266 32.17706470886031 15.237795051669204 12-13 17.572689532597245 25.277809839233832 39.677530996281604 17.471969631887323 14-15 18.71437300891593 27.005222803935787 37.50733480230627 16.77306938484201 16-17 19.48351056161911 27.142022970535546 35.62048712300421 17.753979344841138 18-19 19.609647175924653 27.905727743008296 34.405742469992504 18.078882611074555 20-21 19.94114640977736 27.971188279731063 33.66522742230189 18.422437888189684 22-23 19.56967681951246 30.07024139853313 33.22650424509293 17.13357753686147 24-25 19.37589378681467 29.837579658019642 32.6644299018774 18.122096653288295 26-27 19.46586422518135 29.777863700500756 32.267190236548075 18.48908183776982 28-29 19.782466028729452 30.026918600863194 31.610537114230443 18.58007825617691 30-31 19.55576466625203 30.21364545902973 31.841647238959226 18.388942635759012 32-33 19.200716845920013 30.06283004543337 31.767453812376683 18.968999296269935 34-35 19.984011514952567 30.899297459211635 30.167536884014996 18.949154141820806 36-37 20.07033593349503 30.822875229146575 30.496223321904832 18.610565515453565 38-39 20.134463068791185 30.126678840141285 30.68509846203359 19.05375962903394 40-41 20.339027665861494 29.991313690436805 30.69747431064868 18.97218433305302 42-43 20.044548195613306 30.21437858172218 31.077140896637296 18.66393232602722 44-45 20.415874430869565 29.610726465718983 31.016667620815713 18.95673148259574 46-47 19.9911150708735 29.892628054753757 30.900217037212634 19.216039837160107 48-49 20.05200309567273 29.626865695620126 31.03685261393459 19.284278594772555 50-51 19.61176491716703 30.080032703733572 31.67555257453391 18.63264980456549 52-53 20.519801782091747 30.22398389954155 30.546939467008226 18.709274851358476 54-55 20.20882559925342 30.556823225014856 30.09814714604436 19.136204029687363 56-57 20.342504839373504 30.48172882751966 30.143769785707146 19.03199654739969 58-59 19.8597883495576 31.150141627569795 29.770755307977183 19.219314714895425 60-61 19.838234878582487 31.16897460197935 29.53626402248225 19.456526496955913 62-63 20.398703573950566 31.285146749774484 29.50424334564724 18.81190633062771 64-65 20.546763730365562 30.792054534477746 29.49888431689561 19.162297418261083 66-67 20.32907668193947 31.133593901565405 29.369804551627393 19.167524864867737 68-69 20.710894950039965 30.71081807289969 29.553418121808978 19.024868855251363 70-71 20.242072726618453 31.052731810575896 29.34500992041006 19.360185542395588 72-73 20.019713109973466 30.628478626042956 30.083958685316297 19.26784957866728 74-75 20.37070652898477 30.045755049446726 30.38265588700821 19.200882534560296 76-77 19.984543287863314 31.037651631271977 29.462063013368372 19.515742067496337 78-79 20.571795581938858 30.876089839412746 29.431878278558305 19.120236300090085 80-81 19.927696659109973 31.204224560271403 29.883997776220088 18.984081004398536 82-83 20.681254619229826 31.157401048858464 29.063272693310033 19.098071638601674 84-85 20.487499794298007 30.465555736279278 29.352307933733 19.694636535689718 86-87 20.545277203556093 31.614047716904786 28.93096929640609 18.90970578313303 88-89 20.45882573689518 30.921081148402973 29.570156211134297 19.049936903567552 90-91 20.254848821282053 31.41138703792658 29.23364073326498 19.100123407526386 92-93 20.70707316178102 30.955879347023092 29.1085457986281 19.228501692567786 94-95 20.607893361453385 30.933615959245486 28.962169484089202 19.496321195211923 96-97 20.365313065536075 31.28017994818981 29.295208511826182 19.05929847444793 98-99 20.97913767888813 30.267141027136287 28.950160692953474 19.80356060102211 100-101 21.129473320046635 31.543940557459255 28.30368234795138 19.02290377454273 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5606.0 1 13272.0 2 17979.0 3 17958.5 4 25455.0 5 35560.0 6 47251.0 7 61268.0 8 78726.0 9 97953.0 10 118514.5 11 140659.0 12 163166.5 13 185485.5 14 208800.0 15 234070.0 16 261060.0 17 294295.5 18 335902.5 19 380667.0 20 444724.5 21 570443.0 22 785639.0 23 903675.0 24 977263.5 25 1154187.5 26 1372582.5 27 1668411.5 28 1923846.5 29 2065183.5 30 2217024.5 31 2414164.5 32 2597672.5 33 2796888.5 34 3024383.5 35 3231957.5 36 3437466.0 37 3704815.5 38 3731724.0 39 3433993.0 40 3306038.0 41 3239382.5 42 2948000.5 43 2691726.0 44 2562740.5 45 2457478.5 46 2371307.5 47 2267198.0 48 2150596.5 49 2066216.0 50 1895766.0 51 1672261.5 52 1526343.5 53 1403953.5 54 1268191.0 55 1121913.5 56 988114.5 57 863430.0 58 740441.0 59 633418.5 60 516117.0 61 392917.5 62 294410.5 63 211594.0 64 148881.0 65 108696.0 66 79453.0 67 60568.5 68 47437.0 69 36579.0 70 32677.5 71 34643.0 72 35593.0 73 42132.5 74 44387.0 75 33664.0 76 26541.5 77 25392.5 78 21866.5 79 14648.0 80 9499.0 81 6156.0 82 4226.5 83 2950.5 84 2127.0 85 1680.5 86 1409.0 87 1121.0 88 852.5 89 640.5 90 441.5 91 266.0 92 153.0 93 91.5 94 45.5 95 21.0 96 13.5 97 11.5 98 6.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003757390176602479 2 0.0011963829418037745 3 6.238950106672027E-4 4 7.010056299631491E-6 5 4.988823399904412E-4 6 6.192216398007817E-5 7 3.154525334834171E-4 8 1.635679803247348E-4 9 1.6239963760812952E-4 10-11 0.0019324388532650809 12-13 5.759929592863875E-4 14-15 2.693029961775098E-4 16-17 7.886313337085427E-5 18-19 1.6473632304134003E-4 20-21 1.331910696929983E-4 22-23 6.536877499406365E-4 24-25 5.187441661727303E-4 26-27 2.3658940011256279E-4 28-29 5.941022713937689E-4 30-31 3.429085873236404E-4 32-33 3.119475053336013E-4 34-35 4.095041221701396E-4 36-37 4.2644509156091566E-4 38-39 3.6569127029744276E-4 40-41 3.680279557306533E-4 42-43 4.994665113487437E-4 44-45 2.6170876851957565E-4 46-47 0.004319363023289603 48-49 0.004803641079322478 50-51 0.00985497081456527 52-53 0.004321699708722814 54-55 1.0924004400259073E-4 56-57 2.1380671713876046E-4 58-59 3.399877305321273E-4 60-61 7.886313337085427E-5 62-63 1.9861826182289223E-5 64-65 4.6149537305907315E-5 66-67 2.091333462723395E-4 68-69 2.465203132037074E-4 70-71 1.2150764252694583E-4 72-73 7.01005629963149E-5 74-75 1.3435941240960358E-4 76-77 7.903838477834505E-4 78-79 1.857664919402345E-4 80-81 1.402011259926298E-4 82-83 9.40515886867225E-5 84-85 3.183733902749302E-4 86-87 1.2384432796015634E-4 88-89 1.331910696929983E-4 90-91 1.2851769882657734E-4 92-93 4.2644509156091566E-4 94-95 2.4535197048710213E-4 96-97 2.84491451493378E-4 98-99 3.4641361547345617E-4 100-101 0.1682027958815078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8.5591324E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.497843892838628 #Duplication Level Percentage of deduplicated Percentage of total 1 84.58855480887915 25.797685396820206 2 6.196904310303294 3.779844405489773 3 2.415298596189597 2.2098419862354786 4 1.2981471568954879 1.5836275736372356 5 0.8614517441176386 1.3136210406656654 6 0.6158017694326753 1.1268375739854923 7 0.45388771027637814 0.9689817573020864 8 0.3475757540305841 0.8480248869888345 9 0.2884490124062947 0.7917365656269585 >10 2.3112292609588683 14.229016484112575 >50 0.3201989061136405 6.851157395840615 >100 0.25741006654905085 15.62369197995022 >500 0.026457618875113775 5.5396553984463095 >1k 0.01642114477767545 9.45460839376614 >5k 0.0013249211227037102 2.7809085547419516 >10k+ 8.872190719821325E-4 7.100760606390407 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 237689 0.27770221196718486 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 233396 0.2726865166847986 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 147402 0.17221605311304683 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 141288 0.16507280574372232 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 138138 0.1613925261864158 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 136618 0.15961664525717584 No Hit GTTTTATTTAATCAACATAACCATATCCAATAAAATGCATCTTCAGAAAA 123444 0.1442248983086183 No Hit CCTGGATGGAAATTTGCATCAAACACTACAATAGTCAGTCGGATTTATTG 113607 0.13273191100537246 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 112928 0.1319386063007975 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 106191 0.12406748141902794 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 93123 0.10879957879843054 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11434219664600585 0.0 0.0 0.0 1.1683427166052485E-6 2 0.11498478514013874 3.5050281498157455E-6 0.0 9.346741732841988E-6 1.1683427166052485E-6 3 0.11516120489034613 3.5050281498157455E-6 0.0 1.0515084449447235E-5 1.1683427166052485E-6 4 0.13321677323276365 3.5050281498157455E-6 0.0 1.1683427166052484E-5 1.1683427166052485E-6 5 0.13364906003790758 3.5050281498157455E-6 0.0 1.4020112599262982E-5 2.336685433210497E-6 6 0.1340801785003349 3.5050281498157455E-6 0.0 1.8693483465683976E-5 2.336685433210497E-6 7 0.1350522396405505 3.5050281498157455E-6 0.0 3.62186242147627E-5 2.336685433210497E-6 8 0.14249575108804252 3.5050281498157455E-6 0.0 3.62186242147627E-5 2.336685433210497E-6 9 0.1430238419959481 3.5050281498157455E-6 0.0 3.8555309647973196E-5 3.5050281498157455E-6 10-11 0.1462566462927948 3.5050281498157455E-6 0.0 4.147616643948632E-5 3.5050281498157455E-6 12-13 0.14896077551037767 4.0891995081183695E-6 0.0 5.023873681402569E-5 3.5050281498157455E-6 14-15 0.1515544963412413 5.841713583026242E-6 0.0 6.192216398007817E-5 3.5050281498157455E-6 16-17 0.15521900327187366 5.841713583026242E-6 1.1683427166052485E-6 6.776387756310441E-5 4.0891995081183695E-6 18-19 0.15832796324076023 8.178399016236739E-6 1.1683427166052485E-6 8.7041532387091E-5 5.841713583026242E-6 20-21 0.16165014575542727 1.0515084449447235E-5 1.7525140749078728E-6 1.0631918721107761E-4 7.010056299631491E-6 22-23 0.16451667461061825 1.1683427166052484E-5 2.9208567915131215E-6 1.133292435107091E-4 7.010056299631491E-6 24-25 0.16725702245241586 1.3435941240960358E-5 3.5050281498157455E-6 1.4721118229226132E-4 7.010056299631491E-6 26-27 0.16988520939341936 1.4604283957565606E-5 3.5050281498157455E-6 2.0445997540591849E-4 7.5942276579341155E-6 28-29 0.1725209905620808 1.6356798032473478E-5 3.5050281498157455E-6 2.3074768652953657E-4 8.178399016236739E-6 30-31 0.17504227414451493 1.9861826182289223E-5 3.5050281498157455E-6 2.3951025690407594E-4 8.178399016236739E-6 32-33 0.17716281617515345 2.044599754059185E-5 3.5050281498157455E-6 2.453519704871022E-4 8.178399016236739E-6 34-35 0.17976179454824182 2.103016889889447E-5 4.673370866420994E-6 2.558670549365494E-4 8.178399016236739E-6 36-37 0.18226964218943498 2.1614340257197096E-5 4.673370866420994E-6 3.055216203922725E-4 8.178399016236739E-6 38-39 0.18453622705964917 2.5119368407012844E-5 4.673370866420994E-6 3.131158480502066E-4 8.178399016236739E-6 40-41 0.18685889238026043 2.6287711123618088E-5 4.673370866420994E-6 3.259676179328643E-4 8.178399016236739E-6 42-43 0.18935972996515393 2.920856791513121E-5 5.841713583026242E-6 3.545920144896929E-4 8.178399016236739E-6 44-45 0.19186115172140578 2.920856791513121E-5 5.841713583026242E-6 3.802955542550084E-4 8.178399016236739E-6 46-47 0.19681726152524526 3.037691063173646E-5 6.4258849413288665E-6 4.083357794535343E-4 8.178399016236739E-6 48-49 0.1996101847892901 3.037691063173646E-5 7.010056299631491E-6 4.1651417846977107E-4 8.178399016236739E-6 50-51 0.20246736690274822 3.2713596064946956E-5 7.010056299631491E-6 4.235242347694026E-4 8.178399016236739E-6 52-53 0.20567096263167983 3.505028149815745E-5 7.010056299631491E-6 4.2819760563582356E-4 8.178399016236739E-6 54-55 0.20933313287687896 3.62186242147627E-5 7.010056299631491E-6 4.4455440366829703E-4 8.178399016236739E-6 56-57 0.2131232366495464 3.680279557306533E-5 7.010056299631491E-6 4.597428589841653E-4 9.346741732841988E-6 58-59 0.21719082181740756 3.8555309647973196E-5 7.010056299631491E-6 4.7843634244984924E-4 1.0515084449447235E-5 60-61 0.2213618053156883 4.0307823722881075E-5 7.010056299631491E-6 4.8719891282438855E-4 1.1683427166052484E-5 62-63 0.22560055269153215 4.264450915609156E-5 7.010056299631491E-6 5.041398822151647E-4 1.2267598524355108E-5 64-65 0.2300449283854985 4.4981194589302064E-5 7.010056299631491E-6 5.292592506221775E-4 1.3435941240960358E-5 66-67 0.23499870150390478 4.6733708664209943E-5 8.178399016236739E-6 5.654778748369403E-4 1.4020112599262982E-5 68-69 0.24065464859499075 4.790205138081519E-5 8.178399016236739E-6 5.771613020029928E-4 1.4020112599262982E-5 70-71 0.24691638138463662 4.790205138081519E-5 8.178399016236739E-6 6.063698699181239E-4 1.4020112599262982E-5 72-73 0.25367933319970604 4.965456545572306E-5 8.178399016236739E-6 6.174691257258738E-4 1.4604283957565606E-5 74-75 0.2614809416898376 5.023873681402568E-5 8.178399016236739E-6 6.688762052565047E-4 1.518845531586823E-5 76-77 0.2704876956921475 5.1991250888933554E-5 8.178399016236739E-6 7.722745356760691E-4 1.6940969390776103E-5 78-79 0.2807042685775021 5.257542224723618E-5 8.178399016236739E-6 8.102456739657398E-4 1.7525140749078725E-5 80-81 0.2922878024412848 5.257542224723618E-5 8.178399016236739E-6 9.136440043853043E-4 1.7525140749078725E-5 82-83 0.30602926530263747 5.374376496384143E-5 8.178399016236739E-6 9.96596337264277E-4 1.8693483465683976E-5 84-85 0.32297081886477186 5.54962790387493E-5 8.178399016236739E-6 0.001007111421713724 1.9277654823986598E-5 86-87 0.3439857993083505 5.78329644719598E-5 8.178399016236739E-6 0.0010164581634465662 1.9861826182289223E-5 88-89 0.3688411222614105 6.0169649905170295E-5 9.346741732841988E-6 0.0010281415906126185 1.9861826182289223E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 53655 0.0 66.82877 1 GCGTGTT 67035 0.0 52.90752 2 GTTTTTT 1323240 0.0 42.632202 1 CAAAACG 76385 0.0 40.29437 9 CGTGTTT 103190 0.0 35.041996 3 AGACGGC 15595 0.0 34.934574 9 CCGAAAC 87200 0.0 32.148964 1 CCTTTAG 64650 0.0 31.349785 5 TCGTCTA 67630 0.0 31.198631 44-45 CTAACGA 64240 0.0 30.280914 94-95 ACTCGTC 71900 0.0 29.54461 42-43 TAACGAG 57865 0.0 29.045357 94-95 TAGGCAT 68265 0.0 28.798824 9 CCCGAAA 58085 0.0 28.72754 1 CGAAACC 97695 0.0 28.451479 2 TTTAGGC 71965 0.0 28.24229 7 CTTTGCT 123565 0.0 27.953918 1 GTATCGT 53650 0.0 27.57776 28-29 TATCGTA 52515 0.0 27.178799 30-31 TTGCTTC 113625 0.0 27.108574 3 >>END_MODULE