FastQCFastQC Report
Fri 27 May 2016
SRR1233027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85591324
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA2208450.2580226472486861No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1434540.1676034360678893No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1416240.1654653688965017No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1247850.14579164589158594No Hit
CCTGGATGGAAATTTGCATCAAACACTACAATAGTCAGTCGGATTTATTG1202970.14054812377946158No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA1087840.12709699408318534No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT1030440.12039070688987122No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA958930.11203588812342707No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC891580.10416709992709074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT534050.067.048421
GCGTGTT669500.053.3131032
GTTTTTT12851850.037.231911
CAAAACG659500.037.1044779
CGTGTTT1021800.035.6677443
CCGAAAC759250.033.6929131
TCGTCTA669050.031.28500444-45
CTAACGA613750.030.06142694-95
CCCGAAA484950.029.6193051
CGAAACC859950.029.4721742
ACTCGTC713250.029.3880942-43
CCAAACG956100.028.7593527
TAACGAG545500.027.98130694-95
TAACGGC91750.027.902819
CCTTTAG598300.027.8350455
CAAACGA1047750.026.1384748
GCCTAAC713000.025.9108292-93
GTATCGT437600.025.86732528-29
TAGGCAT636450.025.6272079
CGAGCTA821050.025.45864312-13
Produced by FastQC (version 0.11.5)