FastQCFastQC Report
Fri 27 May 2016
SRR1233026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114736695
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA5633020.490951913858073No Hit
CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC3716430.3239094519848249No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT3703050.3227433037007036No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA3474910.30285951673960976No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT2799020.24395159717647436No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT2392560.2085261389131001No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT1955870.17046595250107213No Hit
CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC1685880.14693468379928495No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1414610.12329185532143835No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA1218260.10617875998607071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT659150.071.016981
GCGTGTT895300.052.2463572
GTTTTTT18730800.042.6887171
CAAAACG951900.039.0954939
CCTTTAG1287550.036.3299065
TAACGGC221450.035.0688139
CGTGTTT1390650.034.257343
TAGGCAT1383050.033.395369
TCGTCTA1599800.033.02791644-45
CCGAAAC2057450.032.792251
ACTCGTC1635900.032.50269342-43
TTAGGCA1433200.032.3693668
TAACGAG1257500.032.0430994-95
CTAACGA1537150.031.84723394-95
CCCGAAA1387850.031.5591531
TTTAGGC1497300.031.5070867
GCCTAAC1712900.029.22524892-93
CGAAACC2318750.029.0365982
AGAGCGT502200.028.9605989
GTCTATG1846800.028.92951246-47
Produced by FastQC (version 0.11.5)