##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 114736695 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.061656464830193 31.0 28.0 33.0 16.0 34.0 2 28.456544778459932 31.0 28.0 33.0 16.0 34.0 3 28.24663049602396 31.0 28.0 33.0 16.0 34.0 4 31.297340785352063 35.0 32.0 37.0 16.0 37.0 5 31.111146673694932 35.0 30.0 37.0 16.0 37.0 6 31.117119296490106 35.0 31.0 37.0 16.0 37.0 7 31.572966102954247 35.0 32.0 37.0 17.0 37.0 8 31.488437940451398 35.0 32.0 37.0 17.0 37.0 9 32.47763606054715 37.0 32.0 39.0 17.0 39.0 10-11 32.04921151859917 37.0 31.0 39.0 13.0 39.0 12-13 31.93202160825706 37.0 30.0 39.0 11.0 39.0 14-15 32.47644724732571 37.0 31.0 40.0 11.0 41.0 16-17 31.95887073006591 37.0 30.0 39.5 10.0 41.0 18-19 31.63413321256988 36.5 30.0 39.5 10.0 41.0 20-21 31.21555993921561 36.0 28.5 39.0 10.0 41.0 22-23 30.774866275344607 36.0 27.0 39.0 5.0 40.0 24-25 30.62903592874102 36.0 27.0 39.0 2.0 41.0 26-27 30.283705901586238 36.0 27.0 40.0 2.0 41.0 28-29 29.902656159827508 36.0 26.5 40.0 2.0 41.0 30-31 29.553175634002706 36.0 25.0 39.0 2.0 41.0 32-33 29.277295589697786 36.0 24.5 39.0 2.0 41.0 34-35 28.975043755618024 36.0 24.0 39.0 2.0 41.0 36-37 28.778020022277964 36.0 24.0 39.0 2.0 41.0 38-39 28.60753958007942 35.0 23.5 39.0 2.0 41.0 40-41 28.45725713120811 35.0 23.0 39.0 2.0 41.0 42-43 28.243260506152804 35.0 21.0 39.0 2.0 40.0 44-45 28.02238671333526 35.0 19.0 39.0 2.0 40.0 46-47 27.80766152885962 35.0 18.5 39.0 2.0 40.0 48-49 27.552899850392237 35.0 17.5 39.0 2.0 40.0 50-51 27.067384213916917 33.5 15.0 38.5 2.0 40.0 52-53 27.12613001446486 34.0 15.0 38.0 2.0 40.0 54-55 27.272049194897935 34.0 15.0 39.0 2.0 40.0 56-57 27.133677159691587 34.0 13.5 39.0 2.0 40.0 58-59 26.845625935102976 34.0 12.0 39.0 2.0 40.0 60-61 26.568791858611583 34.0 8.5 38.5 2.0 40.0 62-63 26.21825522776301 33.5 8.0 38.0 2.0 40.0 64-65 25.85331895345251 33.0 8.0 38.0 2.0 40.0 66-67 25.560257165329716 33.0 7.0 38.0 2.0 40.0 68-69 25.260858978027912 32.0 4.0 37.5 2.0 40.0 70-71 25.05236936622586 32.0 2.0 37.0 2.0 39.0 72-73 24.794654896587353 32.0 2.0 36.5 2.0 39.0 74-75 24.424774632910598 31.5 2.0 36.0 2.0 39.0 76-77 23.949065340430103 31.0 2.0 36.0 2.0 39.0 78-79 23.554862670569342 31.0 2.0 35.0 2.0 38.0 80-81 23.182748849441758 31.0 2.0 35.0 2.0 37.0 82-83 22.833302767697816 30.0 2.0 35.0 2.0 37.0 84-85 22.490503630943877 30.0 2.0 34.5 2.0 36.0 86-87 22.034569951661933 29.5 2.0 34.0 2.0 36.0 88-89 21.67618583139422 29.0 2.0 34.0 2.0 35.5 90-91 21.290885683956645 29.0 2.0 34.0 2.0 35.0 92-93 20.860127036951866 29.0 2.0 34.0 2.0 35.0 94-95 20.353054918480964 29.0 2.0 34.0 2.0 35.0 96-97 19.754516565079726 28.0 2.0 34.0 2.0 35.0 98-99 18.95838601155454 26.5 2.0 34.0 2.0 35.0 100-101 16.743362557201074 20.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5594441243063386 1101 2 1.461465601008463 1101 3 1.5719467984871827 1101 4 1.7541999841133133 1101 5 1.8103792135254935 1101 6 1.885188179294559 1101 7 1.7820404462420356 1101 8 1.8462547403168728 1101 9 2.020482411817703 1101 10-11 2.0678324106878847 1101 12-13 2.034903427962668 1101 14-15 2.2553792067572047 1101 16-17 2.570970679276911 1101 18-19 2.47272293123655 1101 20-21 2.4972350061482267 1101 22-23 2.4603210301465523 1101 24-25 2.419412053624228 1101 26-27 2.4052493680385147 1101 28-29 2.4030352883391615 1101 30-31 2.350615216394697 1101 32-33 2.45455883304934 1101 34-35 2.417484777113522 1101 36-37 2.4832009619522957 1101 38-39 2.478604862752146 1101 40-41 2.49473652036302 1101 42-43 2.53889485258615 1101 44-45 2.56590095744572 1101 46-47 2.418462029359439 1101 48-49 2.5253906728779967 1101 50-51 2.4899992072054786 1101 52-53 2.396017137961447 1101 54-55 2.443499511256558 1101 56-57 2.5567584345486587 1101 58-59 2.6209209167619143 1101 60-61 2.6290622039386626 1101 62-63 2.6035485884583345 1101 64-65 2.57732089343283 1101 66-67 2.6751363388227887 1101 68-69 2.6874906843445316 1101 70-71 2.7077012089785875 1101 72-73 2.624942656128656 1101 74-75 2.6003020433878348 1101 76-77 2.7935274726859447 1101 78-79 2.796808094457603 1101 80-81 2.724032552813817 1101 82-83 2.6836907195764645 1101 84-85 2.718162752232857 1101 86-87 2.698776416920399 1101 88-89 2.6467731707687356 1101 90-91 2.689444640231276 1101 92-93 2.7577498673305207 1101 94-95 2.8954437263034123 1101 96-97 2.9214950663062673 1101 98-99 3.0069339243992097 1101 100-101 3.0652863524687817 1102 1 1.3799039304539313 1102 2 1.3040580857148996 1102 3 1.358382676448759 1102 4 1.5788681732697185 1102 5 1.6360899179151147 1102 6 1.67092814891609 1102 7 1.6029827382442363 1102 8 1.5896346208753513 1102 9 1.7675566183514846 1102 10-11 1.8797133752721749 1102 12-13 1.8959360393723657 1102 14-15 1.931091075730528 1102 16-17 2.2381589221423894 1102 18-19 2.15100725123693 1102 20-21 2.2031639302824395 1102 22-23 2.105769435811858 1102 24-25 2.059874470413469 1102 26-27 2.0379839497597523 1102 28-29 2.117123218310958 1102 30-31 2.0994240839239744 1102 32-33 2.125642204978419 1102 34-35 2.1551611018605428 1102 36-37 2.1455445609753596 1102 38-39 2.1546461351794406 1102 40-41 2.170857505998516 1102 42-43 2.2515903658772736 1102 44-45 2.317881430373877 1102 46-47 2.237213747589319 1102 48-49 2.322436000376527 1102 50-51 2.296821095908051 1102 52-53 2.259394255457593 1102 54-55 2.371520935392713 1102 56-57 2.3685420708889495 1102 58-59 2.3645571768494555 1102 60-61 2.374549199982642 1102 62-63 2.3557139482704663 1102 64-65 2.354549282958569 1102 66-67 2.3207444405694417 1102 68-69 2.4206622945425806 1102 70-71 2.444721752597669 1102 72-73 2.462341870153484 1102 74-75 2.4197015605166854 1102 76-77 2.5206882563149335 1102 78-79 2.5564116512198822 1102 80-81 2.507701517113105 1102 82-83 2.447400464088826 1102 84-85 2.404316141995988 1102 86-87 2.383734274173733 1102 88-89 2.405582355545956 1102 90-91 2.4903913127091393 1102 92-93 2.5207387647612016 1102 94-95 2.6358785003666405 1102 96-97 2.69456377498226 1102 98-99 2.768986806382678 1102 100-101 2.860562296532521 1103 1 1.3807857303540487 1103 2 1.3357878324714534 1103 3 1.386215799040361 1103 4 1.6320593151407934 1103 5 1.7341927652737823 1103 6 1.6439846000587082 1103 7 1.4777157110788792 1103 8 1.4418829101644945 1103 9 1.746004532517503 1103 10-11 1.8427969456551168 1103 12-13 1.8887332070701675 1103 14-15 2.060547878792427 1103 16-17 2.2693866122255386 1103 18-19 2.211549529323829 1103 20-21 2.2239435477348444 1103 22-23 2.2527244066658554 1103 24-25 2.118934176091514 1103 26-27 2.0807243309168406 1103 28-29 2.1323012590812525 1103 30-31 2.1209616428883074 1103 32-33 2.127183272737632 1103 34-35 2.1992029761194516 1103 36-37 2.2009129065346293 1103 38-39 2.2546026974071687 1103 40-41 2.262041442909034 1103 42-43 2.3003963227492967 1103 44-45 2.3366821980552714 1103 46-47 2.392215618987901 1103 48-49 2.419073295825296 1103 50-51 2.3626134308001454 1103 52-53 2.307340239152989 1103 54-55 2.3714154948659996 1103 56-57 2.4008120751847137 1103 58-59 2.4503985613543655 1103 60-61 2.4958803655973902 1103 62-63 2.48003817153214 1103 64-65 2.4327158033213045 1103 66-67 2.4333104223221156 1103 68-69 2.5594007149698257 1103 70-71 2.643756064658273 1103 72-73 2.576573003332424 1103 74-75 2.5931587028207304 1103 76-77 2.7535044620067666 1103 78-79 2.7057980415723932 1103 80-81 2.6440133986287258 1103 82-83 2.6040709373205573 1103 84-85 2.5940685744603655 1103 86-87 2.599373474150184 1103 88-89 2.658088735069125 1103 90-91 2.7448303902544815 1103 92-93 2.7821325955948844 1103 94-95 2.8444602289829604 1103 96-97 2.886394529349129 1103 98-99 3.021706739470659 1103 100-101 3.039533572911431 1104 1 1.2746906229164985 1104 2 1.243819880376872 1104 3 1.2958036175528882 1104 4 1.4912971210630133 1104 5 1.539556153031704 1104 6 1.4845080986320696 1104 7 1.4374080112223453 1104 8 1.447998244707378 1104 9 1.5963128724817466 1104 10-11 1.668704809451242 1104 12-13 1.5876102268826102 1104 14-15 1.811186724597441 1104 16-17 2.033580333910706 1104 18-19 1.9476140856204474 1104 20-21 1.9205323583188907 1104 22-23 1.9712857151310033 1104 24-25 1.922234816779408 1104 26-27 1.8991121268905609 1104 28-29 1.884934004642119 1104 30-31 1.884790661847667 1104 32-33 1.9796411943096501 1104 34-35 2.007216371933474 1104 36-37 2.027329004223038 1104 38-39 2.035942388649456 1104 40-41 2.0176653559245885 1104 42-43 2.114571000676335 1104 44-45 2.123773825522264 1104 46-47 2.127817581087257 1104 48-49 2.2613854419615933 1104 50-51 2.198882182676641 1104 52-53 2.1472487484529594 1104 54-55 2.2448308973976445 1104 56-57 2.2385176482306015 1104 58-59 2.305547205527393 1104 60-61 2.2981496370510186 1104 62-63 2.2523403576464993 1104 64-65 2.2956714172346864 1104 66-67 2.4080000582087067 1104 68-69 2.45149177131195 1104 70-71 2.432853789410874 1104 72-73 2.405750726919422 1104 74-75 2.406575809900424 1104 76-77 2.561993392818657 1104 78-79 2.564744962278663 1104 80-81 2.553485680239479 1104 82-83 2.4766327005294606 1104 84-85 2.510603060982074 1104 86-87 2.5382767659144143 1104 88-89 2.600997472005009 1104 90-91 2.648501847144619 1104 92-93 2.649700465189671 1104 94-95 2.865445061207776 1104 96-97 2.9487114920795676 1104 98-99 3.049839451000963 1104 100-101 3.1329823540022588 1105 1 0.10593729341353963 1105 2 0.057600141859317944 1105 3 0.06105699156955069 1105 4 0.12775896770130046 1105 5 0.16217159690093652 1105 6 0.16701373350020887 1105 7 0.1592802402391662 1105 8 -0.43966501635847166 1105 9 -0.12831329780830458 1105 10-11 0.07221353304652212 1105 12-13 0.09393117192847455 1105 14-15 0.2381663714694966 1105 16-17 0.3897984739383986 1105 18-19 0.19946318800778684 1105 20-21 0.32263966836923785 1105 22-23 0.404667316807231 1105 24-25 0.2272332090141802 1105 26-27 0.19375727654073316 1105 28-29 0.21803558688194968 1105 30-31 0.16282468244850534 1105 32-33 0.18693962029314193 1105 34-35 0.2981132391116219 1105 36-37 0.283523096404906 1105 38-39 0.29906440716559857 1105 40-41 0.33151760957975895 1105 42-43 0.4108784254006146 1105 44-45 0.40221852552909354 1105 46-47 0.4286505922223647 1105 48-49 0.4932002305726897 1105 50-51 0.4331381183346892 1105 52-53 0.34800609090000734 1105 54-55 0.39756522306683806 1105 56-57 0.34811752512588257 1105 58-59 0.33017378861872615 1105 60-61 0.43997845159347904 1105 62-63 0.5194616559817824 1105 64-65 0.45730967340825757 1105 66-67 0.48682790112673047 1105 68-69 0.6030133347337951 1105 70-71 0.6370890639125619 1105 72-73 0.5986697917780646 1105 74-75 0.5885516006274187 1105 76-77 0.7540315244527562 1105 78-79 0.716615204524075 1105 80-81 0.7929055040087505 1105 82-83 0.7596403737255493 1105 84-85 0.7415439024079582 1105 86-87 0.7270435576728325 1105 88-89 0.7700754249513189 1105 90-91 0.8997450723813465 1105 92-93 0.9775074536484958 1105 94-95 1.1301269535105831 1105 96-97 1.2037956974570072 1105 98-99 1.3605025564711113 1105 100-101 1.6702360504066007 1106 1 -0.4521970229106991 1106 2 -0.4806038217856745 1106 3 -0.6206196090799061 1106 4 -0.5684197509633151 1106 5 -0.5283048572465532 1106 6 -0.5407840667753021 1106 7 -0.629714275261609 1106 8 -0.7762199146060347 1106 9 -0.7243517876891232 1106 10-11 -0.6089247222771093 1106 12-13 -0.6318796720447821 1106 14-15 -0.677172774974764 1106 16-17 -0.6350415619104126 1106 18-19 -0.6941441039932101 1106 20-21 -0.5607841923445882 1106 22-23 -0.6381046796507164 1106 24-25 -0.7142730189321469 1106 26-27 -0.7615973080083869 1106 28-29 -0.665329547613748 1106 30-31 -0.6585429119384436 1106 32-33 -0.6775291231205962 1106 34-35 -0.6381754210408772 1106 36-37 -0.6131252667284777 1106 38-39 -0.6122122454843826 1106 40-41 -0.6044127091435563 1106 42-43 -0.613152998104372 1106 44-45 -0.5577178408511294 1106 46-47 -0.5534227872144477 1106 48-49 -0.5517294582012227 1106 50-51 -0.5574234248502634 1106 52-53 -0.6227010342133568 1106 54-55 -0.6694536717508051 1106 56-57 -0.6529572701066151 1106 58-59 -0.628738006964209 1106 60-61 -0.5615777296938482 1106 62-63 -0.5198377095066853 1106 64-65 -0.5932835541484103 1106 66-67 -0.5454773440883756 1106 68-69 -0.4834316736661357 1106 70-71 -0.47214201784137444 1106 72-73 -0.518218992911347 1106 74-75 -0.5094228049575165 1106 76-77 -0.3221087082309211 1106 78-79 -0.34934736428207813 1106 80-81 -0.3210085747067133 1106 82-83 -0.32464652128116356 1106 84-85 -0.35339774054802575 1106 86-87 -0.3696075473775551 1106 88-89 -0.3410829063619545 1106 90-91 -0.22881466265856076 1106 92-93 -0.13048049695386155 1106 94-95 -0.0020461760872834134 1106 96-97 0.02576946878347286 1106 98-99 0.07253041905698154 1106 100-101 0.3387583450950302 1107 1 -0.9875438407245518 1107 2 -0.9175011350350601 1107 3 -0.8319854660295007 1107 4 -0.8921986552140346 1107 5 -1.0523847091132055 1107 6 -1.148456586558897 1107 7 -1.0112848161082901 1107 8 -1.0931815956589084 1107 9 -1.0960187783236215 1107 10-11 -1.251402136589661 1107 12-13 -1.4094295301994606 1107 14-15 -1.4635254588653304 1107 16-17 -1.1440481889346472 1107 18-19 -1.3933302384542152 1107 20-21 -1.3109419173817294 1107 22-23 -1.3851092728920307 1107 24-25 -1.443111544590451 1107 26-27 -1.4686087813581885 1107 28-29 -1.4151782925135308 1107 30-31 -1.3762955676702582 1107 32-33 -1.2886900826986185 1107 34-35 -1.305596551816901 1107 36-37 -1.3392268448860492 1107 38-39 -1.3143235142468015 1107 40-41 -1.3214456462306963 1107 42-43 -1.4637563516690086 1107 44-45 -1.5505336712331044 1107 46-47 -1.4022991559189357 1107 48-49 -1.2934073106570523 1107 50-51 -1.3419725662279163 1107 52-53 -1.3629262088150966 1107 54-55 -1.1926143120509458 1107 56-57 -1.3013418995283992 1107 58-59 -1.258501098548706 1107 60-61 -1.3617293483135278 1107 62-63 -1.4515125550484633 1107 64-65 -1.2974681500612064 1107 66-67 -1.2117357979071173 1107 68-69 -1.2130635691157323 1107 70-71 -1.291053312749753 1107 72-73 -1.230583072694241 1107 74-75 -1.1825390889111702 1107 76-77 -1.0418613566820056 1107 78-79 -1.0940187037815292 1107 80-81 -1.107689466697316 1107 82-83 -1.007466766789797 1107 84-85 -1.0168702344094562 1107 86-87 -0.9681694498924074 1107 88-89 -0.92704916052298 1107 90-91 -0.8041838985156105 1107 92-93 -0.8821189330068968 1107 94-95 -0.9149234164079374 1107 96-97 -0.8353837418134624 1107 98-99 -0.7653044587101476 1107 100-101 -0.39068033555198056 1108 1 -1.095367848174682 1108 2 -0.7683428825252321 1108 3 -1.3741047840565805 1108 4 -1.1607929690950591 1108 5 -1.265021211044001 1108 6 -2.6134445190623588 1108 7 -1.6597015654360234 1108 8 -1.1651885758009293 1108 9 -0.5730801534868917 1108 10-11 -0.6332275398072298 1108 12-13 -0.8424016160440075 1108 14-15 -1.9843931284968832 1108 16-17 -1.4048263644482084 1108 18-19 -2.929039950065249 1108 20-21 -1.425660074411109 1108 22-23 -1.6441580589838622 1108 24-25 -1.4748570029849475 1108 26-27 -1.6655139062268596 1108 28-29 -1.6005192971143316 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2307 34-35 -3.459041394545629 2307 36-37 -3.621514416116135 2307 38-39 -3.751505526352755 2307 40-41 -3.8425827352342985 2307 42-43 -3.6690071515836564 2307 44-45 -3.61359318298544 2307 46-47 -3.645560032687559 2307 48-49 -3.405141434172272 2307 50-51 -3.713224905429893 2307 52-53 -3.5701956924308575 2307 54-55 -3.4931251472035605 2307 56-57 -3.4575264986963354 2307 58-59 -3.4721356972699873 2307 60-61 -3.596018529067024 2307 62-63 -3.4949719555324243 2307 64-65 -3.590947430895067 2307 66-67 -3.625756093709164 2307 68-69 -3.6273516282524696 2307 70-71 -3.5132774772595745 2307 72-73 -3.387827351516979 2307 74-75 -3.3846344959970054 2307 76-77 -3.329813660334736 2307 78-79 -3.5018968247494797 2307 80-81 -3.468502554980585 2307 82-83 -3.434146500202491 2307 84-85 -3.2034129815165393 2307 86-87 -3.191602631262189 2307 88-89 -3.1644150473461004 2307 90-91 -3.022109799656622 2307 92-93 -2.99864902992336 2307 94-95 -2.895727268617648 2307 96-97 -2.803949776771141 2307 98-99 -2.7645871400807955 2307 100-101 -2.297269819353666 2308 1 -3.6236266252961435 2308 2 -4.079786517093513 2308 3 -3.599668434033827 2308 4 -5.232974117529789 2308 5 -6.086946977546841 2308 6 -5.659602612870547 2308 7 -5.032690951263859 2308 8 -4.75517372886647 2308 9 -4.851740916636508 2308 10-11 -5.491415243944694 2308 12-13 -4.985325311352327 2308 14-15 -7.253106294219734 2308 16-17 -7.164656249700627 2308 18-19 -5.918429310618624 2308 20-21 -6.6665492061840474 2308 22-23 -5.2288298532064665 2308 24-25 -6.24775148228521 2308 26-27 -5.621170109904565 2308 28-29 -5.480867841772891 2308 30-31 -6.031331717913449 2308 32-33 -6.268184692089328 2308 34-35 -6.385576729085017 2308 36-37 -5.087643215146638 2308 38-39 -5.459478685627328 2308 40-41 -4.90165932769683 2308 42-43 -5.419320028971146 2308 44-45 -5.689336827306981 2308 46-47 -5.212832779725442 2308 48-49 -4.775203120717023 2308 50-51 -4.940333186881453 2308 52-53 -4.618724771587733 2308 54-55 -5.006699469478374 2308 56-57 -5.3179488906183146 2308 58-59 -4.994161711376346 2308 60-61 -4.620074538545506 2308 62-63 -4.7133758897421 2308 64-65 -4.4620882655972345 2308 66-67 -4.7801750918770765 2308 68-69 -4.980505319145021 2308 70-71 -4.789849271968297 2308 72-73 -4.50741473641752 2308 74-75 -4.52045133953542 2308 76-77 -4.3676692711609135 2308 78-79 -4.615299851748922 2308 80-81 -4.316647333634322 2308 82-83 -4.2925350181813435 2308 84-85 -4.133184771822432 2308 86-87 -4.127274952243006 2308 88-89 -4.087391044696989 2308 90-91 -4.050904554383823 2308 92-93 -3.999711339068284 2308 94-95 -3.926449582386148 2308 96-97 -3.9358829151911436 2308 98-99 -3.9068118019852527 2308 100-101 -3.2948724333515216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4609269.0 3 1251450.0 4 1153560.0 5 1146145.0 6 1287532.0 7 1629476.0 8 2139179.0 9 2645202.0 10 2596941.0 11 2220847.0 12 1746353.0 13 1394538.0 14 1204392.0 15 1111211.0 16 1076617.0 17 1074577.0 18 1096744.0 19 1147124.0 20 1227721.0 21 1332883.0 22 1458788.0 23 1610602.0 24 1788589.0 25 1994349.0 26 2241018.0 27 2532108.0 28 2881298.0 29 3292451.0 30 3793456.0 31 4405081.0 32 5172142.0 33 6140763.0 34 7369264.0 35 8895027.0 36 1.0528482E7 37 1.0825625E7 38 6041722.0 39 674072.0 40 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.124877495030944 15.577932335768383 9.678528831681746 49.61866133751893 2 13.316253189725577 18.793012094548285 55.30702718935784 12.583707526368293 3 17.734849490389763 22.008222551584787 40.71712860868584 19.539799349339614 4 19.34585021884325 29.466395008139028 34.12696962172231 17.060785151295416 5 19.69361638722176 33.62159413008112 32.8779027324215 13.806886750275623 6 15.698168229050784 34.45182399313572 34.58525132029342 15.264756457520065 7 13.2239551487661 17.161493269879767 53.038956655787175 16.575594925566964 8 13.815997180877604 21.160335006455146 44.50197049906108 20.52169731360617 9 15.387853171658056 21.80967787352316 44.497114021776824 18.30535493304196 10-11 18.440427505690717 33.98127342893564 32.5781406993305 15.00015836604314 12-13 17.507934761898575 25.418780443867306 39.988736486505275 17.08454830772885 14-15 18.734088624603668 27.01868702244303 37.99988643525315 16.24733791770016 16-17 19.05160328838155 26.774000309090656 36.46641318281067 17.707983219717125 18-19 19.40306469906942 27.991948935990337 35.04320719527622 17.561779169664028 20-21 19.17499343909358 27.697486464478118 35.178662474194226 17.948857622234083 22-23 18.983201825739354 30.11384697682386 34.40145757724173 16.501493620195056 24-25 18.8394150491249 30.153705922594515 33.38823121610274 17.61864781217785 26-27 18.993250261367987 30.366840870446836 32.71173465226702 17.928174215918155 28-29 19.297795793874894 30.17075290254371 32.46649401279 18.064957290791394 30-31 18.878692079863384 30.298246041790307 32.79064386537772 18.03241801296859 32-33 18.697690677550447 30.28071255781778 32.74760003434315 18.273996730288626 34-35 19.567666351399232 31.229457365455882 30.647435200601624 18.555441082543265 36-37 19.78589134986172 31.241898702122857 30.920252094333243 18.05195785368218 38-39 19.533162326148602 30.585400369823155 31.198518301853507 18.68291900217473 40-41 19.813747657825502 30.68359750769027 30.911548510069338 18.591106324414895 42-43 19.474426069478113 30.846087573717696 31.37961073711913 18.299875619685064 44-45 20.178400679611677 29.755280617409813 31.337667365574795 18.72865133740372 46-47 20.07312943890989 29.73965103753993 31.146888710726593 19.04033081282359 48-49 19.86679758538619 29.78365830643034 31.321978101629412 19.027566006554057 50-51 19.124864774905486 30.319260271391315 32.22492941413284 18.330945539570365 52-53 20.42103529217469 30.314457711285552 31.0070033251934 18.257503671346363 54-55 20.32134146904259 30.46244976848384 30.54493399453424 18.67127476793933 56-57 20.1986779670808 30.68853799624018 30.09195840905695 19.020825627622067 58-59 19.486923686959102 31.83170111783512 29.823697490237 18.857677704968783 60-61 19.554931815561883 31.26238203361772 29.98483626244851 19.197849888371888 62-63 20.18175898460498 31.769614380421274 29.80644465559752 18.242181979376227 64-65 20.430276436382126 31.212368928305846 29.708222484546653 18.649132150765375 66-67 20.245750598751318 31.431904425838024 29.52259774288311 18.799747232527544 68-69 20.242380771492158 31.455792389737482 29.666979922464954 18.63484691630541 70-71 20.188198245531506 31.221923511103693 29.67540591882053 18.91447232454427 72-73 19.730812652312505 30.656664978073817 30.576095192117446 19.036427177496233 74-75 19.675152560751467 29.963303179039215 31.472982241035748 18.888562019173573 76-77 19.644142287897047 31.05151324496971 30.26867895601398 19.035665511119262 78-79 20.493117453762416 31.00475179977018 29.97759140183654 18.524539344630867 80-81 19.63691242447044 31.356347986759904 30.382458707989592 18.624280880780073 82-83 20.537335441729116 31.394720891946154 29.375539510533798 18.692404155790936 84-85 20.398557219535903 30.547591615620355 29.67494645702064 19.378904707823096 86-87 20.12343277481022 31.869701264889088 29.450266390031565 18.55659957026913 88-89 19.976377081467863 31.12417022669464 30.162309437859758 18.737143253977738 90-91 19.855402805551993 31.715003648992706 29.75114836754352 18.67844517791178 92-93 20.45555470732886 30.963504345742138 29.3267696856244 19.254171261304602 94-95 20.354101550864964 30.679481152847053 29.443459020530515 19.522958275757464 96-97 20.231012896515345 31.48457393094383 29.62415023021276 18.660262942328067 98-99 20.601749541693504 30.324504714479694 29.362420811792177 19.71132493203462 100-101 20.919319741906243 31.84249236244156 28.453977740420335 18.784210155231865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4546.0 1 11815.0 2 17982.0 3 20655.5 4 30262.5 5 43564.5 6 60116.0 7 80372.0 8 103857.0 9 130019.0 10 158456.5 11 188394.0 12 219799.5 13 251981.0 14 285335.0 15 320972.0 16 359731.0 17 403924.5 18 457319.0 19 520136.5 20 602666.0 21 742333.0 22 970141.5 23 1126022.5 24 1275270.5 25 1540676.0 26 1906719.0 27 2447015.0 28 2805561.5 29 2912241.0 30 3097877.5 31 3465537.5 32 3745548.0 33 3967168.5 34 4325335.0 35 4771797.5 36 5168061.5 37 5589099.5 38 5498044.0 39 4714711.5 40 4336563.5 41 4256524.5 42 3979547.5 43 3724037.0 44 3545046.0 45 3338056.5 46 3097886.5 47 2899754.0 48 2727141.0 49 2539438.5 50 2290476.0 51 1999155.0 52 1785642.0 53 1599371.5 54 1420233.5 55 1234563.5 56 1053290.0 57 884317.0 58 728867.5 59 603715.5 60 478270.5 61 358284.5 62 266313.0 63 193781.0 64 136244.0 65 99485.5 66 75837.0 67 60203.5 68 49586.5 69 42215.5 70 45839.0 71 58947.5 72 63088.0 73 82443.5 74 88347.0 75 61761.5 76 47255.0 77 45331.5 78 39516.5 79 24807.5 80 14458.0 81 8216.5 82 4734.0 83 2686.0 84 1705.5 85 1272.5 86 1013.5 87 779.0 88 585.0 89 457.5 90 323.5 91 208.0 92 127.0 93 65.5 94 36.0 95 18.0 96 9.5 97 10.5 98 8.5 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0058002367943402935 2 0.0023418837364977264 3 0.0011016527885869469 4 5.22936450278614E-6 5 5.778447775578685E-4 6 6.885329928668418E-5 7 3.564683469399219E-4 8 2.4316544937955553E-4 9 1.8215619684705054E-4 10-11 0.002181516558412285 12-13 6.432118338426952E-4 14-15 3.355508889287773E-4 16-17 1.1417445831083071E-4 18-19 2.680049307677897E-4 20-21 2.2834891662166143E-4 22-23 0.0011931666673857043 24-25 8.197028858117274E-4 26-27 4.1355557609533725E-4 28-29 6.824320676135912E-4 30-31 5.556199784210274E-4 32-33 5.002758707665407E-4 34-35 6.732806797337156E-4 36-37 7.281890070129699E-4 38-39 6.754595816098765E-4 40-41 5.530052961696342E-4 42-43 9.547948021337028E-4 44-45 5.098630390216487E-4 46-47 0.004768744646165728 48-49 0.0052006029980208165 50-51 0.010669646707184654 52-53 0.004901657660611542 54-55 1.8477087909844363E-4 56-57 3.216059169213476E-4 58-59 6.292668618352655E-4 60-61 1.224542854402421E-4 62-63 2.4839481388234163E-5 64-65 5.316520577832576E-5 66-67 3.5995458994177935E-4 68-69 2.8282146352568376E-4 70-71 2.069956782352847E-4 72-73 1.185322620631525E-4 74-75 1.651607622129956E-4 76-77 0.0010232123210451548 78-79 3.303215244259912E-4 80-81 2.1222504273807086E-4 82-83 1.5034422945510152E-4 84-85 5.207575484024532E-4 86-87 2.0220209410773074E-4 88-89 2.0655989786005251E-4 90-91 2.0612411748482034E-4 92-93 5.194502072767566E-4 94-95 3.4862430018574265E-4 96-97 4.7630795012877094E-4 98-99 6.135787683269071E-4 100-101 0.174534833864615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.14736695E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.146062088139864 #Duplication Level Percentage of deduplicated Percentage of total 1 82.91079389758842 27.48166322306434 2 7.148076205319937 4.7386115542457965 3 2.8211372120473 2.805287675690449 4 1.5552077787447338 2.061960543769241 5 1.0033409795468713 1.6628401201817835 6 0.7000492561966909 1.3922325666390991 7 0.5195505921503403 1.2054739329741508 8 0.4040568212270702 1.071431398682312 9 0.3176736817364163 0.9476668420742913 >10 2.155996962964041 13.945062297019787 >50 0.24618275256580224 5.663054555522129 >100 0.18294171799927583 11.914423490680704 >500 0.019711108053520592 4.495007612295317 >1k 0.013170508423978152 8.395200414086421 >5k 0.0011935148777068283 2.70634983366851 >10k+ 9.17010558479017E-4 9.51373393940524 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 563302 0.490951913858073 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 371643 0.3239094519848249 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 370305 0.3227433037007036 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 347491 0.30285951673960976 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 279902 0.24395159717647436 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 239256 0.2085261389131001 No Hit CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT 195587 0.17046595250107213 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 168588 0.14693468379928495 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 141461 0.12329185532143835 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 121826 0.10617875998607071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.12663516236021963 2.61468225139307E-6 0.0 1.4816532757894063E-5 0.0 2 0.12689140122085615 4.357803752321784E-6 0.0 3.137618701671684E-5 0.0 3 0.12701952065117442 6.972486003714853E-6 0.0 3.573399076903862E-5 2.61468225139307E-6 4 0.12840355912291182 1.045872900557228E-5 0.0 4.967896277646833E-5 3.4862430018574267E-6 5 0.12863713740403626 1.045872900557228E-5 0.0 5.577988802971883E-5 3.4862430018574267E-6 6 0.12914612888230745 1.045872900557228E-5 0.0 6.188081328296933E-5 3.4862430018574267E-6 7 0.1293823218456833 1.2201850506500994E-5 0.0 7.84404675417921E-5 3.4862430018574267E-6 8 0.13021727704462813 1.4816532757894063E-5 0.0 8.802763579690002E-5 3.4862430018574267E-6 9 0.1306626445881154 1.830277575975149E-5 0.0 9.587168255107923E-5 3.4862430018574267E-6 10-11 0.13200354080270482 1.9174336510215848E-5 0.0 9.761480405200795E-5 3.4862430018574267E-6 12-13 0.13393056162198153 2.1353238386376737E-5 0.0 1.1199555643466984E-4 3.4862430018574267E-6 14-15 0.1361835461619319 2.2660579512073274E-5 8.715607504643567E-7 1.2768364994302826E-4 3.4862430018574267E-6 16-17 0.1392754079242042 2.5275261763466342E-5 8.715607504643567E-7 1.4424330420185103E-4 3.4862430018574267E-6 18-19 0.14212715469972356 2.7454163639627238E-5 8.715607504643567E-7 1.7997729497088965E-4 3.4862430018574267E-6 20-21 0.1449388096807216 2.876150476532377E-5 1.307341125696535E-6 2.1658284649039264E-4 3.4862430018574267E-6 22-23 0.14745326244581125 2.9197285140555947E-5 1.7431215009287133E-6 2.3357828112444758E-4 3.922023377089605E-6 24-25 0.1498818664769802 3.0940406641484664E-5 1.7431215009287133E-6 2.745416363962724E-4 4.357803752321784E-6 26-27 0.15218627310120794 3.22477477671812E-5 1.7431215009287133E-6 3.2552794029843725E-4 4.357803752321784E-6 28-29 0.15439001445875708 3.399086926810991E-5 1.7431215009287133E-6 3.5254632356283226E-4 4.357803752321784E-6 30-31 0.15652098049364244 3.660555151950298E-5 1.7431215009287133E-6 3.756426834501377E-4 5.22936450278614E-6 32-33 0.1582810974292052 4.183491602228912E-5 1.7431215009287133E-6 3.8828031433187093E-4 5.22936450278614E-6 34-35 0.16034669640780572 4.357803752321783E-5 2.61468225139307E-6 4.126840153448729E-4 6.100925253250497E-6 36-37 0.16212293721725207 4.444959827368219E-5 2.61468225139307E-6 4.5756939399378726E-4 6.972486003714853E-6 38-39 0.16370743466159626 4.7935841275539616E-5 2.61468225139307E-6 4.71514366001217E-4 7.84404675417921E-6 40-41 0.16548018922804078 5.142208427739704E-5 2.61468225139307E-6 4.928676043875937E-4 7.84404675417921E-6 42-43 0.16731874663114532 5.534410765448665E-5 2.61468225139307E-6 5.560557587962595E-4 7.84404675417921E-6 44-45 0.1692279004550375 5.7087229155415364E-5 2.61468225139307E-6 5.874319458129764E-4 1.0022948630340103E-5 46-47 0.17122333879322565 6.188081328296933E-5 2.61468225139307E-6 6.270879599591046E-4 1.1766070131268816E-5 48-49 0.17315863943963175 6.362393478389803E-5 2.61468225139307E-6 6.392898104656056E-4 1.2201850506500994E-5 50-51 0.17504905470738896 6.449549553436239E-5 2.61468225139307E-6 6.628219507281432E-4 1.2201850506500994E-5 52-53 0.17718917213015417 6.754595816098764E-5 2.61468225139307E-6 6.824320676135912E-4 1.2201850506500994E-5 54-55 0.17953584945077944 6.798173853621982E-5 2.61468225139307E-6 7.042210863752002E-4 1.2201850506500994E-5 56-57 0.18210128851977128 6.972486003714854E-5 2.61468225139307E-6 7.286247873882021E-4 1.2201850506500994E-5 58-59 0.1847965901405823 7.05964207876129E-5 2.61468225139307E-6 7.486706846488824E-4 1.307341125696535E-5 60-61 0.18766402500961005 7.408266378947032E-5 2.61468225139307E-6 7.647945585324729E-4 1.3944972007429707E-5 62-63 0.19065957930895605 7.582578529039903E-5 2.61468225139307E-6 7.752532875380453E-4 1.3944972007429707E-5 64-65 0.1938107072022599 8.0619369417953E-5 3.4862430018574267E-6 7.957349651739577E-4 1.3944972007429707E-5 66-67 0.19744075772794395 8.628451429597132E-5 3.4862430018574267E-6 8.301616148172998E-4 1.4816532757894063E-5 68-69 0.20151181799336298 8.846341617213221E-5 3.4862430018574267E-6 8.441065868247295E-4 1.6559654258822776E-5 70-71 0.2062330625786284 9.06423180482931E-5 3.4862430018574267E-6 8.728680915900532E-4 1.6559654258822776E-5 72-73 0.21121011024415512 9.325700029968616E-5 3.4862430018574267E-6 8.933497692259656E-4 1.6559654258822776E-5 74-75 0.21699727362723842 9.500012180061487E-5 3.4862430018574267E-6 9.399782693758087E-4 1.830277575975149E-5 76-77 0.223359231325253 9.587168255107923E-5 3.4862430018574267E-6 0.0010415150968049061 1.9174336510215848E-5 78-79 0.23072043342367496 9.935792555293665E-5 3.4862430018574267E-6 0.0010820426717014987 1.9174336510215848E-5 80-81 0.23900287523533775 1.045872900557228E-4 3.4862430018574267E-6 0.0011713776486240953 1.9174336510215848E-5 82-83 0.24850724521915155 1.0589463118141935E-4 3.4862430018574267E-6 0.0012467676535392624 1.9174336510215848E-5 84-85 0.2606894856087671 1.1025243493374112E-4 3.4862430018574267E-6 0.0012716071349274964 2.0481677635912382E-5 86-87 0.27570734890001847 1.1199555643466984E-4 3.4862430018574267E-6 0.0012942677144395696 2.091745801114456E-5 88-89 0.29322964200772905 1.146102386860629E-4 3.4862430018574267E-6 0.001309520027572696 2.1353238386376737E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 65915 0.0 71.01698 1 GCGTGTT 89530 0.0 52.246357 2 GTTTTTT 1873080 0.0 42.688717 1 CAAAACG 95190 0.0 39.095493 9 CCTTTAG 128755 0.0 36.329906 5 TAACGGC 22145 0.0 35.068813 9 CGTGTTT 139065 0.0 34.25734 3 TAGGCAT 138305 0.0 33.39536 9 TCGTCTA 159980 0.0 33.027916 44-45 CCGAAAC 205745 0.0 32.79225 1 ACTCGTC 163590 0.0 32.502693 42-43 TTAGGCA 143320 0.0 32.369366 8 TAACGAG 125750 0.0 32.04309 94-95 CTAACGA 153715 0.0 31.847233 94-95 CCCGAAA 138785 0.0 31.559153 1 TTTAGGC 149730 0.0 31.507086 7 GCCTAAC 171290 0.0 29.225248 92-93 CGAAACC 231875 0.0 29.036598 2 AGAGCGT 50220 0.0 28.960598 9 GTCTATG 184680 0.0 28.929512 46-47 >>END_MODULE