##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 114736695 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.131612541218832 31.0 27.0 31.0 10.0 34.0 2 27.482926843936024 31.0 27.0 31.0 15.0 34.0 3 25.362713506781766 30.0 22.0 31.0 10.0 34.0 4 28.788308108404202 33.0 27.0 37.0 10.0 37.0 5 29.69709212035435 35.0 28.0 37.0 10.0 37.0 6 30.248879000741656 35.0 30.0 37.0 10.0 37.0 7 30.796048875209454 35.0 32.0 37.0 11.0 37.0 8 30.70379294087214 35.0 31.0 37.0 10.0 37.0 9 31.685886899566 37.0 31.0 39.0 10.0 39.0 10-11 31.413064621566797 37.0 30.0 39.0 8.0 39.0 12-13 31.305917230751678 37.0 30.0 39.0 5.0 39.0 14-15 31.895985072604716 37.0 30.5 40.0 2.0 41.0 16-17 31.555768200400056 37.0 30.0 40.0 2.0 41.0 18-19 31.031072173553543 36.5 29.5 40.0 2.0 41.0 20-21 30.57443541492981 36.0 28.5 39.5 2.0 40.0 22-23 30.41188993198732 36.0 28.5 40.0 2.0 41.0 24-25 30.15061294470788 36.0 27.0 40.0 2.0 41.0 26-27 29.71890400015444 36.0 27.0 40.0 2.0 41.0 28-29 29.29527621917295 36.0 25.5 40.0 2.0 41.0 30-31 28.975368294336874 36.0 24.5 40.0 2.0 41.0 32-33 28.69337758072951 36.0 24.0 40.0 2.0 41.0 34-35 28.373058510182815 36.0 22.0 39.5 2.0 40.0 36-37 28.149544986457908 35.0 20.5 39.0 2.0 40.0 38-39 28.01072009264342 35.0 19.0 39.0 2.0 40.0 40-41 27.818938574969412 35.0 18.5 39.0 2.0 40.0 42-43 27.55761657157721 35.0 16.0 39.0 2.0 40.0 44-45 27.56144443153082 35.0 16.0 39.0 2.0 40.0 46-47 27.40348541937695 35.0 15.0 39.0 2.0 40.0 48-49 27.23960827004822 35.0 14.5 39.0 2.0 40.0 50-51 27.058147142899664 34.0 10.5 39.0 2.0 40.0 52-53 26.712832768975957 34.0 9.0 39.0 2.0 40.0 54-55 26.53085120675648 34.0 8.0 38.5 2.0 40.0 56-57 26.286280967043716 33.5 7.5 38.0 2.0 40.0 58-59 26.012066427397095 33.0 4.0 38.0 2.0 40.0 60-61 25.677911051037334 33.0 2.0 38.0 2.0 40.0 62-63 25.236866082816835 32.5 2.0 38.0 2.0 40.0 64-65 24.9720014202954 32.0 2.0 38.0 2.0 40.0 66-67 24.56811320475982 32.0 2.0 37.0 2.0 39.5 68-69 24.120993222787185 31.0 2.0 36.5 2.0 39.0 70-71 23.773169420646113 31.0 2.0 36.0 2.0 39.0 72-73 23.425187970596504 31.0 2.0 36.0 2.0 39.0 74-75 23.0011999212632 30.0 2.0 35.0 2.0 38.0 76-77 22.379080171343613 29.5 2.0 34.0 2.0 37.0 78-79 22.54227240029879 30.0 2.0 35.0 2.0 37.0 80-81 22.2740141765457 30.0 2.0 34.5 2.0 37.0 82-83 21.94705454083369 29.5 2.0 34.0 2.0 36.0 84-85 21.53126097540111 29.0 2.0 34.0 2.0 36.0 86-87 21.03903920624522 29.0 2.0 34.0 2.0 35.5 88-89 20.615113111807865 29.0 2.0 34.0 2.0 35.0 90-91 20.18225704949929 28.5 2.0 34.0 2.0 35.0 92-93 19.591378486193975 26.5 2.0 34.0 2.0 35.0 94-95 18.98948441037107 26.0 2.0 33.5 2.0 35.0 96-97 18.35038099624536 25.0 2.0 33.0 2.0 35.0 98-99 17.515842459990676 22.0 2.0 33.0 2.0 35.0 100-101 14.903680444168277 2.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.5438614704794773 1101 2 2.514876884519307 1101 3 4.611901434315744 1101 4 4.233668224487406 1101 5 3.0185617822391073 1101 6 2.482130296750576 1101 7 2.245101557120581 1101 8 2.172762765948807 1101 9 2.4263400670505 1101 10-11 2.2846040297394943 1101 12-13 2.259247541658059 1101 14-15 2.3671677673450517 1101 16-17 2.487368336785252 1101 18-19 2.4998015748942812 1101 20-21 2.4850779900517423 1101 22-23 2.432400473011878 1101 24-25 2.500506947401398 1101 26-27 2.3921527011437185 1101 28-29 2.4252347116542303 1101 30-31 2.435860233620609 1101 32-33 2.425198989134042 1101 34-35 2.572253588839928 1101 36-37 2.5285291828782555 1101 38-39 2.538789926759936 1101 40-41 2.4935902695657433 1101 42-43 2.6049275833315804 1101 44-45 2.575996275903748 1101 46-47 2.6154269601646973 1101 48-49 2.5622564886286234 1101 50-51 2.6403232235200704 1101 52-53 2.692483378917604 1101 54-55 2.6680527351453307 1101 56-57 2.7428531261629665 1101 58-59 2.6712310956471335 1101 60-61 2.6853504697138746 1101 62-63 2.8695391350651356 1101 64-65 2.7123278488212463 1101 66-67 2.762151921458866 1101 68-69 2.781348406815944 1101 70-71 2.600768379266782 1101 72-73 2.6314554368386425 1101 74-75 2.673481794853192 1101 76-77 2.7827425362622478 1101 78-79 2.93415588256482 1101 80-81 2.9328107597240134 1101 82-83 2.90697501933019 1101 84-85 2.81856466124837 1101 86-87 2.899758603424779 1101 88-89 2.851606664450536 1101 90-91 2.8811042519313013 1101 92-93 2.9738121372639092 1101 94-95 3.006046129038694 1101 96-97 3.0396358848509664 1101 98-99 3.2091224941922327 1101 100-101 3.128588038034966 1102 1 2.3047238674081356 1102 2 2.281229784666049 1102 3 3.7940550143542886 1102 4 3.8104544438269876 1102 5 2.6709692644938734 1102 6 2.254198763617751 1102 7 1.9779357088099054 1102 8 1.9457151654687443 1102 9 2.16669409372712 1102 10-11 2.0282930946445212 1102 12-13 2.024997685333883 1102 14-15 2.120060660902517 1102 16-17 2.088242596655583 1102 18-19 2.0858510429038795 1102 20-21 2.1408948059245176 1102 22-23 2.1529703127769615 1102 24-25 2.1938117726673525 1102 26-27 2.247117109803856 1102 28-29 2.1682928232053236 1102 30-31 2.183772229452451 1102 32-33 2.1018501699425975 1102 34-35 2.1478551318972023 1102 36-37 2.2229949932585384 1102 38-39 2.2462114049028443 1102 40-41 2.1642497275780457 1102 42-43 2.269932141114431 1102 44-45 2.269102093053416 1102 46-47 2.3105274852192643 1102 48-49 2.256781880972145 1102 50-51 2.2963863709557586 1102 52-53 2.423480649151845 1102 54-55 2.350830327298297 1102 56-57 2.4154855886367272 1102 58-59 2.431201923275946 1102 60-61 2.4625385488104286 1102 62-63 2.630019331424048 1102 64-65 2.5045069502704997 1102 66-67 2.549324167875465 1102 68-69 2.568783696162928 1102 70-71 2.4751523177361747 1102 72-73 2.476166324239962 1102 74-75 2.443676933269707 1102 76-77 2.516828346186635 1102 78-79 2.6626302476098225 1102 80-81 2.6437760132113546 1102 82-83 2.632779593420562 1102 84-85 2.5783517753788736 1102 86-87 2.573760076270034 1102 88-89 2.700983996678982 1102 90-91 2.7055432221151463 1102 92-93 2.811634723622742 1102 94-95 2.8658277022802885 1102 96-97 2.783085588091378 1102 98-99 2.9030426028008414 1102 100-101 2.824341874375815 1103 1 1.9465927069527602 1103 2 1.988792023438247 1103 3 0.6800124352630768 1103 4 2.5353152523494025 1103 5 2.0464308295944953 1103 6 1.8081975216159307 1103 7 1.7501249166211643 1103 8 1.770358423081614 1103 9 2.0387502997398066 1103 10-11 1.9465120114377363 1103 12-13 1.9514874357315968 1103 14-15 2.074668144813053 1103 16-17 2.1122352658620613 1103 18-19 2.1948650466336197 1103 20-21 2.202771433324898 1103 22-23 2.238627675850136 1103 24-25 2.180667952395133 1103 26-27 2.1748154163907607 1103 28-29 2.1968774158818256 1103 30-31 2.1691896687418044 1103 32-33 2.16768922118543 1103 34-35 2.1957574119330943 1103 36-37 2.1714793345471577 1103 38-39 2.227219915925634 1103 40-41 2.2347434791649405 1103 42-43 2.320169914666778 1103 44-45 2.2872905225300144 1103 46-47 2.290853079323689 1103 48-49 2.289147323521057 1103 50-51 2.3465038029388303 1103 52-53 2.471871410648202 1103 54-55 2.3672613363620947 1103 56-57 2.4937678243720036 1103 58-59 2.5436708490058955 1103 60-61 2.5494855185868026 1103 62-63 2.747031750900671 1103 64-65 2.65039939231902 1103 66-67 2.6926754944975464 1103 68-69 2.7394809865676386 1103 70-71 2.6628385033622486 1103 72-73 2.7147014203385282 1103 74-75 2.694356907864101 1103 76-77 2.757031753503451 1103 78-79 2.8836138222280248 1103 80-81 2.893830651712406 1103 82-83 2.886223988934507 1103 84-85 2.830380384339289 1103 86-87 2.895683929042555 1103 88-89 2.9637003911414617 1103 90-91 2.973151443071302 1103 92-93 3.0832153423009387 1103 94-95 3.2821188735297895 1103 96-97 3.314479775089218 1103 98-99 3.37944341469667 1103 100-101 3.232081272272193 1104 1 1.6829848115698347 1104 2 1.7625075722427006 1104 3 0.49325956896454315 1104 4 1.4366058900836016 1104 5 1.5310270659163834 1104 6 1.4283205974824789 1104 7 1.4465802903676241 1104 8 1.4468026493351722 1104 9 1.7548407605821659 1104 10-11 1.7053839530883046 1104 12-13 1.729003835210566 1104 14-15 1.8199795473961373 1104 16-17 1.8356595034332415 1104 18-19 1.92265852373772 1104 20-21 1.9406536189496038 1104 22-23 1.8965830003574702 1104 24-25 1.8884594806002681 1104 26-27 1.903370228602821 1104 28-29 1.9043158628569508 1104 30-31 1.8431393545664356 1104 32-33 1.837820998621563 1104 34-35 1.9285858376595115 1104 36-37 1.911384056857063 1104 38-39 1.8952776696891398 1104 40-41 1.9313829194164782 1104 42-43 2.0546890464542606 1104 44-45 1.9978136343461124 1104 46-47 1.951694160066765 1104 48-49 2.0075006262543518 1104 50-51 1.9867610322856493 1104 52-53 2.083681334741744 1104 54-55 2.0941444550206256 1104 56-57 2.1251342301048233 1104 58-59 2.1261063494771655 1104 60-61 2.149691202908997 1104 62-63 2.3531514118895878 1104 64-65 2.271173988517205 1104 66-67 2.286704818653188 1104 68-69 2.3267372531480426 1104 70-71 2.290961995436817 1104 72-73 2.3363952562767594 1104 74-75 2.263892092950652 1104 76-77 2.347194165271283 1104 78-79 2.4905309558468645 1104 80-81 2.4654229626176516 1104 82-83 2.431222058494267 1104 84-85 2.3848328583130076 1104 86-87 2.484113941209806 1104 88-89 2.5617470758131553 1104 90-91 2.541433091329271 1104 92-93 2.663054186118927 1104 94-95 2.8315675201859953 1104 96-97 2.917972738381046 1104 98-99 2.976772902535668 1104 100-101 2.866473058900997 1105 1 -1.9633527952312164 1105 2 -0.6361922302940002 1105 3 -1.9937622503827512 1105 4 -2.1783688520890223 1105 5 -1.149741028792299 1105 6 -0.7583406180348362 1105 7 -0.5298740997918614 1105 8 -0.4614130040528437 1105 9 -0.34559325208984504 1105 10-11 -0.4086895658716152 1105 12-13 -0.4153250491202414 1105 14-15 -0.41435421202755407 1105 16-17 -0.43713413794776557 1105 18-19 -0.29589715217032264 1105 20-21 -0.31197471722661163 1105 22-23 -0.3451756177243226 1105 24-25 -0.29293296944222647 1105 26-27 -0.28454859083172224 1105 28-29 -0.19346959932390817 1105 30-31 -0.17197099757015977 1105 32-33 -0.12136342662678956 1105 34-35 0.004897227518057434 1105 36-37 0.009082740449034787 1105 38-39 -0.08690883413404649 1105 40-41 -0.028359093316492334 1105 42-43 0.10749758281270516 1105 44-45 -0.013426692591529843 1105 46-47 0.02923973398572599 1105 48-49 0.04286436984281394 1105 50-51 0.02582889561392321 1105 52-53 0.17517395619502452 1105 54-55 0.15131025876091186 1105 56-57 0.11718750324136096 1105 58-59 0.16542508128130962 1105 60-61 0.30583034488281413 1105 62-63 0.4603713726040617 1105 64-65 0.3104054493993189 1105 66-67 0.35338514329428605 1105 68-69 0.40186358758279184 1105 70-71 0.35386533287599065 1105 72-73 0.40752248037991023 1105 74-75 0.3805656452588728 1105 76-77 0.4142460944126043 1105 78-79 0.45164558550073863 1105 80-81 0.45769815655217627 1105 82-83 0.5011427644150892 1105 84-85 0.4865250902363769 1105 86-87 0.5676333615096425 1105 88-89 0.6642577340007989 1105 90-91 0.6557825209877599 1105 92-93 0.7785121684801055 1105 94-95 0.9237995044173957 1105 96-97 0.9950598733611962 1105 98-99 1.2120582607344303 1105 100-101 1.3100896975820326 1106 1 -1.058603386020092 1106 2 -0.6858847441158105 1106 3 -3.5100408025780645 1106 4 -5.691063549027227 1106 5 -3.025056240949432 1106 6 -1.9219360493960096 1106 7 -1.4788479154365888 1106 8 -1.295105730022609 1106 9 -1.2326795849213887 1106 10-11 -1.2159132364555738 1106 12-13 -1.2356426399409521 1106 14-15 -1.3056731169453357 1106 16-17 -1.3812237621701229 1106 18-19 -1.1656673689642325 1106 20-21 -1.2885673466707033 1106 22-23 -1.3383118989271559 1106 24-25 -1.300297711078791 1106 26-27 -1.3964024475858459 1106 28-29 -1.4559873746646979 1106 30-31 -1.4184072538953778 1106 32-33 -1.4357985702702187 1106 34-35 -1.4382336123699702 1106 36-37 -1.3772463655224136 1106 38-39 -1.423107113437279 1106 40-41 -1.4606057890196134 1106 42-43 -1.3518183596094246 1106 44-45 -1.3864497695749733 1106 46-47 -1.367215049738654 1106 48-49 -1.3997099401027207 1106 50-51 -1.334145276851487 1106 52-53 -1.228723805525398 1106 54-55 -1.3382776800905987 1106 56-57 -1.2770125087147655 1106 58-59 -1.3162166636812067 1106 60-61 -1.229796084306706 1106 62-63 -1.085698200527549 1106 64-65 -1.1929949029280422 1106 66-67 -1.1895197206392503 1106 68-69 -1.143842074675728 1106 70-71 -1.181359640414005 1106 72-73 -1.1267062939644 1106 74-75 -1.111234837050148 1106 76-77 -0.9770085493614218 1106 78-79 -0.9558329553497167 1106 80-81 -0.952530884693843 1106 82-83 -0.9011711521339691 1106 84-85 -0.9209217688940967 1106 86-87 -0.8440797714320212 1106 88-89 -0.705431157962277 1106 90-91 -0.7037115272829979 1106 92-93 -0.5688884828852565 1106 94-95 -0.49537185285442575 1106 96-97 -0.4682708719700699 1106 98-99 -0.2995642607027271 1106 100-101 -0.11964591363385679 1107 1 -0.7409629640124145 1107 2 -1.5622016138286305 1107 3 -0.8686901786514376 1107 4 -1.639207400096371 1107 5 -1.422600248796595 1107 6 -1.4285698741064472 1107 7 -1.4097637178577997 1107 8 -1.420871074379658 1107 9 -1.4326490737158544 1107 10-11 -1.4805432655354913 1107 12-13 -1.5307174848313494 1107 14-15 -1.6394892304721438 1107 16-17 -1.6583823769292962 1107 18-19 -1.661875707104084 1107 20-21 -1.74361860874086 1107 22-23 -1.7230035749046984 1107 24-25 -1.7510398720575928 1107 26-27 -1.7972847449821039 1107 28-29 -1.7736536710654498 1107 30-31 -1.7724262631593284 1107 32-33 -1.8556618661251107 1107 34-35 -1.7188423468540677 1107 36-37 -1.818447039302086 1107 38-39 -1.8220501380343066 1107 40-41 -1.7944009095181173 1107 42-43 -1.645246345436341 1107 44-45 -1.7690903063125702 1107 46-47 -1.8477668018180324 1107 48-49 -1.8321067131348663 1107 50-51 -1.7887259387021324 1107 52-53 -1.6813549797668514 1107 54-55 -1.7555006206920396 1107 56-57 -1.6378121437807316 1107 58-59 -1.6746302543696068 1107 60-61 -1.615905287285912 1107 62-63 -1.4560864363504713 1107 64-65 -1.6463077419315368 1107 66-67 -1.5576224654981239 1107 68-69 -1.5703429070109323 1107 70-71 -1.554287576234067 1107 72-73 -1.455344152347017 1107 74-75 -1.5578709160172473 1107 76-77 -1.5060450812580264 1107 78-79 -1.474607932120616 1107 80-81 -1.3455827245306473 1107 82-83 -1.3513311412323823 1107 84-85 -1.4773882147437725 1107 86-87 -1.4033502323599691 1107 88-89 -1.2540454598775703 1107 90-91 -1.2659185943391655 1107 92-93 -1.18088183615086 1107 94-95 -1.0586848655614851 1107 96-97 -1.1094760401996169 1107 98-99 -1.0876826566388296 1107 100-101 -0.9252377751176866 1108 1 0.7903430428849774 1108 2 0.7625497332039046 1108 3 2.4806692890376034 1108 4 1.6923986659847436 1108 5 0.5631117468361886 1108 6 0.04374835300295388 1108 7 -0.036285148492428476 1108 8 -0.12762081058908592 1108 9 -0.13054018041872695 1108 10-11 -0.5451540077306909 1108 12-13 -0.4341134854516149 1108 14-15 -0.5402233584824003 1108 16-17 -0.44121992811471955 1108 18-19 -0.34026159166456793 1108 20-21 -0.2884839409505453 1108 22-23 -0.3433186286187855 1108 24-25 -0.33825642005985657 1108 26-27 -0.2760765172278532 1108 28-29 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50-51 -4.034013106560714 2307 52-53 -4.028671935309408 2307 54-55 -4.05878796671556 2307 56-57 -3.993987661221194 2307 58-59 -4.018887465339358 2307 60-61 -4.077589047393612 2307 62-63 -3.950373228447802 2307 64-65 -4.04330029409822 2307 66-67 -4.002861209956524 2307 68-69 -3.988542899373165 2307 70-71 -4.015737070953222 2307 72-73 -3.903828452688959 2307 74-75 -3.884189582649629 2307 76-77 -3.8537436245089296 2307 78-79 -3.932451992353254 2307 80-81 -3.8262762034040847 2307 82-83 -3.864254052920767 2307 84-85 -3.8492533115997176 2307 86-87 -3.80422645089309 2307 88-89 -3.7092157438276736 2307 90-91 -3.726260069131296 2307 92-93 -3.687590639305686 2307 94-95 -3.6130668540639412 2307 96-97 -3.6013238663889044 2307 98-99 -3.509889224630996 2307 100-101 -2.812752662770496 2308 1 -3.4215393429155263 2308 2 -5.814865098169534 2308 3 -4.4367455480018485 2308 4 -7.828756453958249 2308 5 -5.581034083627436 2308 6 -4.824545826974813 2308 7 -4.550408581700939 2308 8 -4.569005807010505 2308 9 -4.591221807974424 2308 10-11 -4.726789621767033 2308 12-13 -4.808628519359413 2308 14-15 -5.049035940512297 2308 16-17 -5.106118404537369 2308 18-19 -5.079788872598794 2308 20-21 -5.002726063132798 2308 22-23 -5.060782099002012 2308 24-25 -5.110341415441404 2308 26-27 -5.0684736174034875 2308 28-29 -4.983631791238576 2308 30-31 -5.069726370178582 2308 32-33 -5.007124272271309 2308 34-35 -4.922331173801158 2308 36-37 -4.835254856483083 2308 38-39 -4.810082225360691 2308 40-41 -4.835883882467705 2308 42-43 -4.7319082790551334 2308 44-45 -4.864932084837115 2308 46-47 -4.863850928246691 2308 48-49 -4.841080199815103 2308 50-51 -4.805223805164548 2308 52-53 -4.728029783594085 2308 54-55 -4.8089175196807155 2308 56-57 -4.776981940899798 2308 58-59 -4.7021035384900465 2308 60-61 -4.761697389325214 2308 62-63 -4.652746563286527 2308 64-65 -4.756577412372614 2308 66-67 -4.652751146929479 2308 68-69 -4.459064513937118 2308 70-71 -4.522462617046127 2308 72-73 -4.457663452701432 2308 74-75 -4.34362252804495 2308 76-77 -4.137713111747562 2308 78-79 -4.210840990541843 2308 80-81 -4.2831262881887255 2308 82-83 -4.219853785539261 2308 84-85 -4.13654308114182 2308 86-87 -4.006886735212085 2308 88-89 -4.007667008769836 2308 90-91 -3.9941028625879724 2308 92-93 -3.7849294088321024 2308 94-95 -3.638738658086826 2308 96-97 -3.5370289041481815 2308 98-99 -3.422875735899858 2308 100-101 -2.745278326196269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8851198.0 3 1843171.0 4 1203128.0 5 1126748.0 6 1569250.0 7 1510041.0 8 1517813.0 9 1939517.0 10 2263983.0 11 1954006.0 12 1511587.0 13 1207728.0 14 1081536.0 15 1048405.0 16 1051089.0 17 1058015.0 18 1068838.0 19 1086400.0 20 1116847.0 21 1171548.0 22 1261602.0 23 1393369.0 24 1573959.0 25 1806439.0 26 2099873.0 27 2454894.0 28 2873725.0 29 3364870.0 30 3955369.0 31 4653327.0 32 5516201.0 33 6621470.0 34 7978636.0 35 9512660.0 36 1.0723396E7 37 9585405.0 38 3958351.0 39 222296.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.7909560348115 16.466144463610675 10.371890061165058 47.37100944041277 2 13.311105748532365 19.10156204852933 55.687255035147366 11.900077167790934 3 19.15515845835193 21.919475634457157 41.465806159299895 17.459559747891017 4 20.203150187697396 30.06746549352865 34.29915157676569 15.43023274200826 5 19.337180675049176 33.78633572465881 33.42999493156753 13.446488668724479 6 15.2493539409818 34.73858861489305 35.26934102415934 14.742716419965818 7 12.995162794177476 17.141465840409666 53.67256370374628 16.190807661666582 8 13.648941895641597 20.982302824008247 45.2697253695392 20.099029910810952 9 15.368817624470104 21.57244440665863 45.06753781990745 17.991200148963816 10-11 18.24738293711092 33.75676595853077 33.24261834042527 14.753232763933042 12-13 17.222902845662137 25.250783816844773 40.77846223121382 16.74785110627927 14-15 18.340273694020564 26.83388727598235 38.69175790456794 16.13408112542914 16-17 18.712184877493293 26.749698801441724 37.12893358203754 17.409182739027443 18-19 19.418055086875384 27.685963384449604 35.57680986640538 17.31917166226964 20-21 18.992555941305937 27.52092420742637 35.7422832961477 17.744236555119993 22-23 18.740222541000687 29.90338334913904 34.92369525630783 16.432698853552445 24-25 18.69847436846972 29.83249331542411 33.83547025311424 17.633562062991928 26-27 18.908856156907845 29.95735950055335 33.24550561030321 17.888278732235598 28-29 19.124394873360536 30.03340710086989 32.78257596265571 18.059622063113856 30-31 18.82855421807664 30.160398096933278 32.95558543990048 18.0554622450896 32-33 18.622777304910414 30.35800184965086 32.72305743234665 18.296163413092067 34-35 19.480606861043363 31.04519422658911 30.742079392313688 18.73211952005384 36-37 19.871519506112616 31.149497347634497 30.96811661322514 18.01086653302775 38-39 19.53053345218393 30.389401039355807 31.48864951212968 18.59141599633058 40-41 19.70674638930292 30.66432429360022 31.171254075917904 18.457675241178954 42-43 19.347967958977673 30.604302457550304 31.757561157628622 18.290168425843405 44-45 19.852201894787036 29.629387299912676 31.807338159228742 18.711072646071546 46-47 19.96741220533061 29.565699191767074 31.500433374996323 18.966455227905993 48-49 19.58778822691991 29.68335242105391 31.862513560848083 18.866345791178098 50-51 19.139019396877753 30.066680263968642 32.49517827353574 18.29912206561787 52-53 20.58876670397341 29.989833816457935 31.046164744751454 18.375234734817205 54-55 20.40274857210031 30.156870582043393 30.728507424399993 18.711873421456303 56-57 20.33714535134602 30.31262031985125 30.377269167489366 18.972965161313365 58-59 19.670471864282387 31.36266840185684 30.08737763787813 18.879482095982635 60-61 19.830399008703463 30.722160996879722 30.085277224699574 19.36216276971724 62-63 20.380928568980238 31.2188818612407 30.15192363011179 18.248265939667277 64-65 20.595695081906072 30.890331794358733 29.883096288666717 18.630876835068474 66-67 20.363349475322785 31.17580360045626 29.699548684646654 18.761298239574298 68-69 20.46486414090137 31.109194778943255 29.82137600165459 18.60456507850079 70-71 20.36715934121477 31.041455814758795 29.84758666185864 18.743798182167794 72-73 20.033107448011087 30.409161313654554 30.625612534930376 18.93211870340398 74-75 20.085565825400582 29.706542877859192 31.41789294895954 18.78999834778068 76-77 19.575938650917625 30.91319414858122 30.51579003720311 18.995077163298042 78-79 20.35219923680014 30.904344606626687 30.27355250529214 18.469903651281037 80-81 19.5705659459358 31.213050668916374 30.63662570951264 18.579757675635186 82-83 20.427951342432042 31.23263409204249 29.782184747808866 18.557229817716603 84-85 20.25894606240733 30.507426618181928 30.010417134904632 19.22321018450611 86-87 19.989847959604788 31.78439317347996 29.74064886753504 18.48510999938021 88-89 19.87490443703371 31.07979521086539 30.40691059911978 18.638389752981123 90-91 19.737085025691698 31.57609244125354 30.10563711968211 18.58118541337265 92-93 20.305374249170566 30.9040020750578 29.67917251121421 19.11145116455742 94-95 20.15886824055579 30.68210361112318 29.714097458733907 19.444930689587125 96-97 20.049399431318648 31.34532893107761 29.967994133114807 18.637277504488935 98-99 20.388077000765477 30.329574528568422 29.732542744417913 19.549805726248188 100-101 20.22300191671785 31.927698194970507 29.165532456409398 18.683767431902247 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4466.0 1 8529.0 2 12597.5 3 15636.0 4 23686.5 5 35495.5 6 50423.5 7 69679.5 8 93662.0 9 121123.5 10 151186.5 11 183400.5 12 218319.5 13 254496.5 14 292061.5 15 333270.0 16 376972.0 17 426907.5 18 487240.0 19 555508.0 20 640687.0 21 779549.5 22 1003453.5 23 1171984.5 24 1332264.5 25 1603825.5 26 1980864.5 27 2515184.5 28 2872234.5 29 2984353.0 30 3162605.0 31 3474785.5 32 3762709.5 33 4050742.5 34 4419730.5 35 4818718.0 36 5214769.0 37 5643799.5 38 5486820.5 39 4695478.0 40 4283026.5 41 4190919.0 42 3950642.0 43 3685924.5 44 3501561.0 45 3290774.0 46 3054953.5 47 2853858.0 48 2682623.0 49 2488933.0 50 2228582.0 51 1948098.0 52 1740662.5 53 1558694.5 54 1380480.0 55 1194839.5 56 1014677.5 57 846264.0 58 694343.0 59 572745.5 60 451108.0 61 337047.0 62 250469.0 63 181607.5 64 127650.5 65 93907.0 66 72459.0 67 58539.5 68 48933.5 69 42571.5 70 47805.0 71 62005.0 72 65154.5 73 80795.5 74 85450.5 75 59855.5 76 45011.0 77 41433.0 78 36303.0 79 24215.5 80 14103.0 81 7800.0 82 4270.0 83 2431.5 84 1618.5 85 1212.5 86 967.5 87 741.0 88 505.0 89 377.0 90 295.5 91 201.0 92 133.0 93 76.0 94 33.0 95 13.0 96 6.0 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01733447176598559 2 0.006520145974223853 3 0.0028744073550314485 4 0.4022566625263173 5 8.924782084755011E-4 6 0.0022617001474550054 7 0.0012628915274228527 8 0.005145694670741561 9 0.007113678845290079 10-11 0.024068585904448443 12-13 0.0245231048358156 14-15 0.03954227546819263 16-17 0.019972250377265965 18-19 0.0035285136982549483 20-21 0.002890967009290271 22-23 0.007985239595754437 24-25 0.021662642452791585 26-27 0.02146131191943432 28-29 0.04462129574152367 30-31 0.04432583664711625 32-33 0.023478539276384075 34-35 0.030174740522201725 36-37 0.02535195910950721 38-39 0.013626852333510216 40-41 0.016673392936758374 42-43 0.017980298282079678 44-45 0.028105655300599344 46-47 0.023157369139837958 48-49 0.02261612991379959 50-51 0.026247487780609334 52-53 0.04366301469638811 54-55 0.046764899407290755 56-57 0.04374886343030885 58-59 0.02644663941209044 60-61 0.02434399910159518 62-63 0.007401729673318549 64-65 0.027103796217940568 66-67 0.03501582471065599 68-69 0.026407854958694774 70-71 0.02606271690151089 72-73 0.02656647901527929 74-75 0.02383500762332399 76-77 0.005667759560269711 78-79 0.006771591250732819 80-81 0.0036208991378041703 82-83 1.8869290247553323E-4 84-85 2.2006908949225007E-4 86-87 0.003568169712401076 88-89 0.006419916487920452 90-91 0.005354433470477775 92-93 0.007255307467240537 94-95 0.009747971213568598 96-97 0.004013537255888363 98-99 0.007304550649641773 100-101 0.16818594957785737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.14736695E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.7829653344303 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4920282658963 27.868253312736197 2 7.2766746422591915 4.916552943787406 3 2.8833474979227542 2.9222408570832226 4 1.5916964942182377 2.1508890994843584 5 1.0666503014941144 1.801730507966765 6 0.7144073304575391 1.448087884770596 7 0.5473475031090304 1.294371520639355 8 0.41896057225069405 1.1322984391070623 9 0.32415876278636174 0.9855939821457127 >10 2.231020111673617 14.66471434481121 >50 0.24120356227709164 5.663425966008754 >100 0.17956306668392494 11.96738380900086 >500 0.018960966640146876 4.415856899840046 >1k 0.01208950153057652 7.8549705024834005 >5k 0.001079284551947053 2.507197839043256 >10k+ 8.121362486804652E-4 8.406432091091489 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 508466 0.44315900854560963 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 319168 0.2781743016042078 No Hit CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA 258159 0.22500125177912786 No Hit CTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGC 250384 0.2182248669442675 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 248633 0.21669876407020439 No Hit CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT 173992 0.15164459809479436 No Hit CTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCAC 167827 0.14627142606818158 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 165348 0.14411082696778044 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 149205 0.13004122177303434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10846312071303779 8.715607504643567E-7 0.0 1.045872900557228E-5 0.0 2 0.10879169911596286 4.357803752321784E-6 0.0 2.0045897260680203E-5 0.0 3 0.10900958930357894 5.22936450278614E-6 0.0 2.7889944014859413E-5 0.0 4 0.11054004998139436 6.972486003714853E-6 0.0 3.573399076903862E-5 0.0 5 0.11090000457133614 6.972486003714853E-6 0.0 4.270647677275348E-5 0.0 6 0.11163124404097573 6.972486003714853E-6 0.0 4.7935841275539616E-5 0.0 7 0.11191188660262526 7.84404675417921E-6 0.0 6.275237403343369E-5 0.0 8 0.1129743191574413 9.587168255107924E-6 0.0 7.408266378947032E-5 0.0 9 0.113617530991284 1.6559654258822776E-5 0.0 8.889919654736438E-5 8.715607504643567E-7 10-11 0.11512445952883688 1.7431215009287135E-5 0.0 9.282121992445398E-5 8.715607504643567E-7 12-13 0.11753911858799837 1.7431215009287135E-5 0.0 1.0327994893002626E-4 8.715607504643567E-7 14-15 0.12019912199841559 1.830277575975149E-5 0.0 1.2201850506500994E-4 8.715607504643567E-7 16-17 0.12354373637832256 2.0045897260680206E-5 0.0 1.425001827009223E-4 8.715607504643567E-7 18-19 0.12647523096250943 2.571104213869852E-5 0.0 1.7082590709101392E-4 8.715607504643567E-7 20-21 0.12917445460669752 2.9633065515788127E-5 0.0 2.1396816423899955E-4 8.715607504643567E-7 22-23 0.13146927406267017 3.0504626266252485E-5 0.0 2.2617001474550054E-4 8.715607504643567E-7 24-25 0.13360851992468498 3.1811967391949015E-5 0.0 2.680049307677897E-4 1.7431215009287133E-6 26-27 0.13539304056126072 3.704133189473516E-5 0.0 3.3903713193063475E-4 2.61468225139307E-6 28-29 0.1371100152396755 3.922023377089605E-5 0.0 3.6344083294363673E-4 2.61468225139307E-6 30-31 0.1387502925720494 4.0527574896592584E-5 8.715607504643567E-7 3.817436087033882E-4 2.61468225139307E-6 32-33 0.1399700418423243 4.270647677275348E-5 8.715607504643567E-7 3.974317022117466E-4 3.4862430018574267E-6 34-35 0.14144167216948333 4.357803752321783E-5 8.715607504643567E-7 4.2532164622660607E-4 4.357803752321784E-6 36-37 0.14258167363109076 4.444959827368219E-5 8.715607504643567E-7 4.8720245950957536E-4 5.6651448780183185E-6 38-39 0.14353080328834641 4.444959827368219E-5 8.715607504643567E-7 5.05941015644559E-4 6.100925253250497E-6 40-41 0.1445949789646634 4.575693939937873E-5 8.715607504643567E-7 5.316520577832576E-4 6.100925253250497E-6 42-43 0.14577158597779027 4.7935841275539616E-5 8.715607504643567E-7 6.092209645745853E-4 6.100925253250497E-6 44-45 0.1468945920047636 5.403676652879012E-5 8.715607504643567E-7 6.488769787207135E-4 6.972486003714853E-6 46-47 0.14804374485425087 5.970191140680843E-5 8.715607504643567E-7 7.085788901275219E-4 6.972486003714853E-6 48-49 0.14919856284861613 6.318815440866586E-5 8.715607504643567E-7 7.294963481386666E-4 7.408266378947032E-6 50-51 0.15040785338988544 6.493127590959457E-5 8.715607504643567E-7 7.543358295269007E-4 7.84404675417921E-6 52-53 0.151891685567551 6.580283666005892E-5 8.715607504643567E-7 7.800468716655992E-4 7.84404675417921E-6 54-55 0.1535075591989119 6.711017778575546E-5 8.715607504643567E-7 8.136019605584769E-4 8.715607504643567E-6 56-57 0.15536093313477436 6.885329928668418E-5 1.7431215009287133E-6 8.33212077443925E-4 8.715607504643567E-6 58-59 0.1573184585803173 7.364688341423813E-5 2.61468225139307E-6 8.523864139541408E-4 8.715607504643567E-6 60-61 0.15943853010582187 7.626156566563122E-5 2.61468225139307E-6 8.741754327157498E-4 9.151387879875745E-6 62-63 0.16180743222558397 7.669734604086339E-5 3.0504626266252484E-6 8.968360122278231E-4 9.587168255107924E-6 64-65 0.1643467244720619 8.323405166934606E-5 3.4862430018574267E-6 9.425929516272018E-4 9.587168255107924E-6 66-67 0.16740285224356516 9.06423180482931E-5 3.4862430018574267E-6 9.844278676494908E-4 1.045872900557228E-5 68-69 0.17100196236260773 9.151387879875745E-5 3.4862430018574267E-6 0.0010027306434092424 1.045872900557228E-5 70-71 0.17520462830134684 9.325700029968616E-5 3.4862430018574267E-6 0.0010371572930525845 1.0894509380804458E-5 72-73 0.1796609184184711 9.587168255107923E-5 3.4862430018574267E-6 0.001062868335191283 1.1330289756036637E-5 74-75 0.18503452622545907 9.935792555293667E-5 4.357803752321784E-6 0.00115830423736713 1.2201850506500994E-5 76-77 0.19106485505792198 1.0110104705386537E-4 4.357803752321784E-6 0.0013622494529757895 1.2201850506500994E-5 78-79 0.1980504144728938 1.0327994893002626E-4 4.357803752321784E-6 0.0014276165092606164 1.2201850506500994E-5 80-81 0.20593586036272005 1.085093134328124E-4 4.357803752321784E-6 0.0015875479069708256 1.2201850506500994E-5 82-83 0.21500662887317784 1.0894509380804458E-4 4.357803752321784E-6 0.0017235113840432653 1.2201850506500994E-5 84-85 0.22670907506966276 1.1155977605943765E-4 4.357803752321784E-6 0.0017439930616791777 1.350919163219753E-5 86-87 0.24110333664395683 1.1330289756036637E-4 4.357803752321784E-6 0.0017631673981893937 1.3944972007429707E-5 88-89 0.2579017985484069 1.146102386860629E-4 5.22936450278614E-6 0.0017871353188271635 1.4816532757894063E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 66965 0.0 72.07801 1 GCGTGTT 90175 0.0 53.697754 2 GTTTTTT 1863465 0.0 37.765713 1 TAACGGC 20640 0.0 35.691006 9 CGTGTTT 139855 0.0 35.276382 3 CAAAACG 82400 0.0 34.64208 9 CCGAAAC 173625 0.0 33.626987 1 TCGTCTA 151380 0.0 32.603085 44-45 CCTTTAG 120655 0.0 31.946636 5 ACTCGTC 154895 0.0 31.875551 42-43 CTAACGA 140605 0.0 31.157099 94-95 CCCGAAA 115365 0.0 31.129894 1 TAACGAG 117545 0.0 30.585192 94-95 TAGGCAT 127280 0.0 29.808119 9 CGAAACC 195780 0.0 29.46171 2 TTAGGCA 130810 0.0 29.083517 8 CCAAACG 216410 0.0 28.90747 7 GCCTAAC 153030 0.0 28.367277 92-93 TTTAGGC 138475 0.0 28.156229 7 GTCTATG 177035 0.0 27.983665 46-47 >>END_MODULE