FastQCFastQC Report
Thu 26 May 2016
SRR1161564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31252645
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8893582.8457047395508446No Hit
AGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAA1051700.3365155173266135No Hit
GAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAA676900.21658966785051315No Hit
GGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAA507090.16225506673115186No Hit
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA475560.1521663206426208No Hit
TGATGCGCATCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG466350.14921937007251707Illumina RNA PCR Primer (100% over 34bp)
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG422440.13516935926543178Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA388340.12425828277894557No Hit
GGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAA379680.1214873173134626No Hit
GGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAAA365050.11680611353055076No Hit
GGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAA349550.11184653330942068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA122150.032.5097431
ACCTAGC63650.030.11652418
TACCTAG64550.029.41763717
TAAGTAG65100.029.20366132
TAGTTGG66300.028.74295836
GTACCTA66850.028.47282416
ATGTACC67000.028.30883414
GTACTAT74050.025.97765226
CCTAGCT74300.025.82988519
CTATAAG75550.025.67033829
GAACTTA87750.025.02523413
ATAAGTA76900.024.9857531
TGGTCGC51200.024.91834345
TAACTAC94100.024.82104311
AACTACC94500.024.66812112
CTAGCTG80150.024.2253220
TACCAGT95000.024.0857215
CTAACTA97100.023.9616710
ACCAGTC95750.023.92051916
GTAGTTG87050.023.88167435
Produced by FastQC (version 0.11.5)