FastQCFastQC Report
Thu 26 May 2016
SRR1161564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31252645
Sequences flagged as poor quality0
Sequence length51
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2469950.7903171075600162No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1209730.38708083747791583No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA804060.25727742403882936No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA783320.25064118573003985No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA695500.22254116411586922No Hit
GCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA627300.20071901114289686No Hit
CATCAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500930.16028403355939955No Hit
CATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA446360.1428231114518467No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC444780.14231755424220896RNA PCR Primer, Index 6 (100% over 42bp)
TGATGCGCATCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT373170.11940429362058795RNA PCR Primer, Index 6 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC1424200.044.95162
ACACGAG1255550.044.9043052
GACACGA1248800.044.8916971
ACGACAT1314200.044.8907621
TAGGTCG1356450.044.8629761
CGACTCC732100.044.856362
AGCCATC1243900.044.853231
GTGTCGC1491500.044.839562
GCTTGCG697750.044.8138851
CGACATC1384250.044.8117142
ATGTGTC871250.044.7962381
CACGAGC253550.044.7757763
GTGTTCA1474150.044.7642672
GGTCGCT394950.044.761533
GCTTGTC1059950.044.7537271
CGTGTTC1435300.044.7534871
TGATGCG3255000.044.739611
CACGAGG497950.044.7353823
GACTCCG289850.044.711513
CTTGGAG837150.044.700912
Produced by FastQC (version 0.11.5)