FastQCFastQC Report
Thu 26 May 2016
SRR1161563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30956532
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8274612.6729770634514227No Hit
AGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAA998760.3226330391272511No Hit
GAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAA642120.20742633574070893No Hit
GGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAA484040.15636118412747269No Hit
TGATGCGCATCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG466840.15080500619384626Illumina RNA PCR Primer (100% over 34bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA451250.14576891235749534No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG417660.13491821370688423Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA364240.1176617587525631No Hit
GGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAA359240.11604659074860194No Hit
GGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAAA350900.11335249051799472No Hit
GGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAA335440.10835839104974679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA122800.031.9526821
TACCTAG60200.030.2363117
ACCTAGC62000.029.28608918
TAAGTAG62500.028.7260432
GTACCTA63050.028.68900316
TAGTTGG63600.028.37070836
ATGTACC65250.027.8266814
CTATAAG70900.025.79720729
GAACTTA89200.025.52640313
AACTACC92450.025.43257512
GTACTAT71500.025.4236926
TGGTCGC53050.025.32150745
ATAAGTA72750.024.86344331
TAACTAC94300.024.86241311
CCTAGCT73500.024.85705619
TACCAGT96400.024.27161615
ACCAGTC97450.024.12564516
CTAACTA98000.023.99388910
CTAGCTG78550.023.4881420
CTTATTC100700.023.3478031
Produced by FastQC (version 0.11.5)