FastQCFastQC Report
Thu 26 May 2016
SRR1161563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30956532
Sequences flagged as poor quality0
Sequence length51
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2089970.6751305346477441No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1003130.3240446959627131No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA677140.21873897244045296No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649880.20993307648285667No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA582650.18821552750159481No Hit
GCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA516230.16675963573697467No Hit
CATCAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA429120.13862017877196323No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC425910.13758324091342017RNA PCR Primer, Index 6 (100% over 42bp)
TGATGCGCATCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT373730.1207273476240814RNA PCR Primer, Index 6 (100% over 39bp)
CATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA372760.12041400503131293No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC1329450.044.9108542
GACACGA1178000.044.8673481
TAGGTCG1260500.044.8586271
ACACGAG1192950.044.847192
ACGACAT1224350.044.8395461
AGCCATC1169900.044.8317641
ATGTGTC816450.044.7973251
GTGTCGC1444050.044.76412
CGACATC1302050.044.7585032
GCTTGCG644700.044.7554861
GTGTTCA1409050.044.748522
GCTTGTC995050.044.7422031
CGACTCC703400.044.736912
CGTGTTC1354850.044.7277641
TGATGCG3191450.044.7247541
GACTCCG281550.044.7240263
CACGAGC247900.044.7223633
GTCGCAG274100.044.7104264
GGTCGCA403850.044.6972963
CACGAGG490750.044.67823
Produced by FastQC (version 0.11.5)